ライフサイエンスコーパス: U7 snRNP

1 U7 snRNP contains a unique Sm ring in which the canonica

2 U7 snRNP contains three novel proteins, Lsm10 and Lsm11,

3 U7 snRNP is one of the key factors that determines the c

4 arrest in early G(1), suggesting that active U7 snRNP is necessary to allow progression through G(1)

5 d the mechanisms underlying HLB assembly and U7 snRNP biogenesis remain unclear.

6 Thus, the HLB concentrates FLASH and U7 snRNP, promoting efficient histone mRNA biosynthesis

7 nti-SLBP antibody demonstrated that SLBP and U7 snRNP form a stable complex only in the presence of p

8 ajority of RNA polymerase II transcripts and U7-snRNP-dependent cleavage for replication-dependent hi

9 In the nucleus, both U7 snRNP and SLBP are present in coiled bodies, structur

10 stone pre-mRNAs are cleaved at the 3' end by U7 snRNP consisting of two core components: a ~60-nucleo

11 In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the h

12 ved at the 3' end by a complex that contains U7 snRNP, the FLICE-associated huge protein (FLASH) and

13 Here, we assembled core U7 snRNP bound to FLASH from recombinant components and

14 tion and functional properties as endogenous U7 snRNP, and accurately cleaves histone pre-mRNAs in a

15 n factors are associated with the endogenous U7 snRNP and are recruited in a U7-dependent manner to h

16 on a spliceosomal Sm site but the engineered U7 snRNP is functionally impaired.

17 tabilizing HLB interactions and facilitating U7 snRNP assembly.

18 e pre-mRNA 3'-end processing by facilitating U7-snRNP recruitment through physical interaction with t

19 be considered as a hallmark of a functional U7 snRNP.

20 n factors, forming catalytically active holo U7 snRNP.

21 We demonstrate that semi-recombinant holo U7 snRNP reconstituted in this manner has the same compo

22 w that distinct domains of FLASH involved in U7 snRNP binding, histone pre-mRNA cleavage, and HLB loc

23 rocessing by promoting stable association of U7 snRNP with the pre-mRNA.

24 w that knocking down the known components of U7 snRNP by RNA interference results in a reduction in c

25 The active form of U7 snRNP contains the HLB component FLASH (FLICE-associa

26 terminus required for efficient formation of U7 snRNP.

27 0 and Lsm11 increases the cellular levels of U7 snRNP but has no effect on histone pre-mRNA processin

28 ression, consistent with our observations of U7 snRNP distributions.

29 ad are required for efficient recruitment of U7 snRNP to histone pre-mRNA.

30 one pre-mRNA processing but has no effect on U7 snRNP levels.

31 ind that failure to concentrate FLASH and/or U7 snRNP in the HLB impairs histone pre-mRNA processing.

32 substrates containing a properly positioned U7 snRNP binding site.

33 In addition to reconstituted U7 snRNP, Xenopus hairpin-binding protein SLBP1 is neces

34 end processing of histone pre-mRNA requires U7 snRNP, which binds downstream of the cleavage site an

35 ic components Lsm11 and U7 snRNA, we rescued U7 snRNP levels and processing defects in SUMO2 knockout

36 ires the U7 small nuclear ribonucleoprotein (U7 snRNP) and an unidentified heat-labile factor (HLF).

37 ucleotide sequence; and a small nuclear RNP, U7 snRNP.

38 in the majority of histone mRNAs, stimulates U7-snRNP-dependent cleavage.

39 NP to histone pre-mRNA substrates stimulates U7-snRNP-dependent cleavage in vitro and in vivo.

40 ed by only 49 nucleotides in the DNA and the U7 snRNP binding sites separated by only 20 nucleotides.

41 unoprecipitation we show that coilin and the U7 snRNP can form a weak but specific complex in the nuc

42 The colocalization of SLBP1 and the U7 snRNP in the coiled body suggests coordinated control

43 which binds the stem-loop sequence, and the U7 snRNP that interacts with a sequence downstream from

44 t both on the hairpin binding factor and the U7 snRNP.

45 le 3' endonucleolytic cleavage guided by the U7 snRNP that binds downstream of the cleavage site.

46 one pre-mRNAs at the 3' end is guided by the U7 snRNP, which is a component of a larger 3'-end proces

47 essential processing factors, including the U7 snRNP, are concentrated.

48 other processing factor(s), most likely the U7 snRNP, to facilitate histone pre-mRNA processing.

49 to chromosomes at the histone gene loci, the U7 snRNP is thus brought close to the actual site of his

50 rate, potentially due to concealment of the U7 snRNP binding element.

51 increased dynamics and reduced levels of the U7 snRNP complex.

52 f FLASH interacts with the N terminus of the U7 snRNP protein Lsm11, and together they recruit the HC

53 protein that helps stabilize binding of the U7 snRNP to the histone pre-mRNA by interacting with the

54 s of the SLBP is to stabilize binding of the U7 snRNP to the histone pre-mRNA.

55 Recruitment of the U7 snRNP to the pre-mRNA also depends on the 20-amino-ac

56 RNAs, reveal an unexpected complexity of the U7 snRNP, and suggest that in animal cells polyadenylati

57 on is catalyzed by CPSF73 and depends on the U7 snRNP and its integral component, Lsm11.

58 vitro by a nuclease that also depends on the U7 snRNP.

59 y a 5'-3' exonuclease, also dependent on the U7 snRNP.

60 By overexpressing the U7 snRNP-specific components Lsm11 and U7 snRNA, we resc

61 Recently, the U7 snRNP has been shown to contain a unique Sm core that

62 -mRNA to form the unique 3' end requires the U7 snRNP and the stem-loop binding protein (SLBP) that b

63 ilin is to form a transient complex with the U7 snRNP and accompany it to the CBs.

64 itment through physical interaction with the U7-snRNP-specific component Lsm11.

65 Thus, U7 snRNP is required only to initiate the degradation.

66 CPSF-73 and requires the interaction of two U7 snRNP-associated proteins, FLASH and Lsm11.

67 splicing and sarcomere length in vitro using U7 snRNPs that truncate the region of titin altered in i

68 ely the Drosophila counterpart of vertebrate U7 snRNP.

69 the interaction of the histone pre-mRNA with U7 snRNP.

70 n found in coiled bodies, is associated with U7 snRNPs.