ライフサイエンスコーパス: UMP

1 UMP also significantly increased plasma and brain cytidi

2 UMP and CMP proved to be far better substrates than dCMP

3 UMP and GMP, accompanied by Mg2+, bind specifically to P

4 UMP and phosphoribosylpyrophosphate were shown by equili

5 UMP synthase, the bifunctional protein that catalyzes th

6 UMP was created in 2014 to unify all trades in the agric

7 UMP was released from the enzyme concomitantly with CO(2

8 UMP, GMP, and CMP could not substitute for AMP as gamma-

9 UMP, which acted as a competitive inhibitor of UDP-Glc,

10 UMP/CMPK localized predominantly to the cytoplasm.

11 UMP/CMPK used all of the nucleoside triphosphates as pho

12 erase (E-His(166) + UDP-glucose = E-His(166)-UMP + glucose-1-P) is found to be 1.8 x 10(-4) at pH 7.0

13 ) for the hydrolyses of UMPIm and E-His(166)-UMP are -14.7 and -15.4 kcal mol(-1), respectively at pH

14 active site and the UMP group of E-His(166)-UMP provide little or no stabilization in the formation

15 ease A (RNaseA), and uridine-3'-phosphate (3'UMP).

16 3'-UMP binds to the variant protein with 5-fold weaker affi

17 66A RNase A and the K7A/R10A/K66A RNase A.3'-UMP complex were determined by X-ray diffraction analysi

18 and with bound 3'-uridine monophosphate (3'-UMP).

19 of 3'-UMP shows that a second molecule of 3'-UMP can bind to the enzyme.

20 Finally, the affinity of 3'-UMP for wild-type RNase A and the K7A/R10A/K66A variant

21 s consistent with the phosphoryl group of 3'-UMP interacting more strongly with the two active-site h

22 e presence of different concentrations of 3'-UMP shows that a second molecule of 3'-UMP can bind to t

23 e reaction product, uridine 3'-phosphate (3'-UMP), protonation of one active-site histidine residue f

24 f wild-type RNase A and the variants with 3'-UMP at pH 6.0 show that His12 and His119 contribute 1.4

25 te (5'-AMP) and uridine 5'-monophosphate (5'-UMP) are synthesized, and their structures are elucidate

26 Among aged animals consuming a UMP-containing diet (2.5%, w/w) for 1 or 6 weeks, baseli

27 In addition, 196-amino acid UMP/CMPK was the actual form of UMP/CMPK, rather than th

28 lactate), DHODH, and UMPS but did not affect UMP levels, likely because of compensatory mechanisms in

29 CMP, although with lower apparent affinity; UMP and the purine nucleotides are poor substrates.

30 d CDP-choline were all elevated 15 min after UMP (from 254 +/- 31.9 to 417 +/- 50.2, [P < 0.05]; 56.8

31 In contrast, although UMP analogs are more slowly incorporated, the excision o

32 ornithine, which function as activators, and UMP, which acts as an inhibitor.

33 and p(5)U in 58% and 70% yield from AMP and UMP, respectively.

34 o be a competitive inhibitor of both ATP and UMP.

35 eeks, a diet containing choline chloride and UMP (a uridine source) and/or DHA by gavage.

36 mplates containing ribavirin support CMP and UMP incorporation with equivalent efficiency.

37 on to OMPDC-catalyzed reactions OMP/FOMP and UMP/FUMP.

38 N-acetyl-l-alanine, 2-hydroxyglutarate, and UMP levels.

39 the release of the two products, Lipid I and UMP.

40 lated by the metabolites ornithine, IMP, and UMP.

41 mechanism by which AHR regulates lactate and UMP production in MYC-overexpressing cells.

42 A structural comparison between MoSto and UMP kinase provides valuable insight into how an enzyme

43 Surprisingly, ornithine and UMP each had a significant effect on chCPS activity, and

44 When both ornithine and UMP were varied, models which presume a mutually exclusi

45 or the allosteric regulators, ornithine, and UMP.

46 o form 2,3-diacylglucosamine 1-phosphate and UMP by catalyzing the attack of water at the alpha-P ato

47 ducts, 2,3-diacylglucosamine 1-phosphate and UMP, by LpxH.

48 yield 2,3-diacylglucosamine 1-phosphate and UMP.

49 ndividually ornithine strongly promotes, and UMP strongly antagonizes, the binding of MgADP.

50 trations were required for the response, and UMP showed no stimulation at all.

51 Binding of PyrR to pyr RNA was specific and UMP-dependent with apparent Kd values of 10 and 220 nM i

52 ternary complex composed of UTP, TbTUT4, and UMP, which mimics an RNA substrate, and the postreaction

53 llular UTP or its breakdown products UDP and UMP act as mediators for hyaluronan synthase (HAS) activ

54 syltransferase activity of PyrR, antagonized UMP-dependent transcriptional termination, but uracil di

55 e slow deuridylylation of GlnK approximately UMP by the UTase/UR suggests that rapid interconversion

56 wed the deuridylylation of PII approximately UMP upon ammonia addition, showing that multiple PII int

57 ng enzyme (UTase/UR), only PII approximately UMP was effectively deuridylylated by the UR activity of

58 alidation of a new luminescence-based assay (UMP-Glo) for measuring activities of PGT enzymes.

59 revented by phosphorothioate substitution at UMP residues in the nascent RNA.

60 nstant = 23.2 microm), and the T-state binds UMP exclusively (dissociation constant = 0.49 microm).

61 the behavior of bisalanyl-AMP and bisalanyl-UMP in the presence of model nucleophiles.

62 We further found that sCPS bound both UMP and IMP and that chCPS bound IMP, although none of t

63 However, both UMP (Km = 153 microM) and CMP (Km = 266 microM) were equ

64 ers for deuterium exchange into enzyme-bound UMP and F-UMP of 1.2 x 10(-5) and 0.041 s(-1), respectiv

65 The 3' hydroxyl of the bound UMP is poised for in-line nucleophilic attack while cont

66 active triphosphate metabolite, PSI-7409, by UMP-CMP kinase and nucleoside diphosphate kinase, respec

67 terminator structures, and that PyrR acts by UMP-dependent binding to and stabilization of the anti-a

68 steps in de novo synthesis are catalysed by UMP synthase (UMPS) - a bifunctional enzyme comprising o

69 w kinetic studies on UTP binding followed by UMP incorporation into an EC as monitored by alterations

70 yrimidines, and its activity is inhibited by UMP and activated by 5-phospho-alpha-D-ribosyl diphospha

71 lites from both pathways, with inhibition by UMP and activation by IMP and ornithine.

72 ressed as a bifunctional gene product called UMP synthase (UMPS), with OPRT at the N terminus and OMP

73 ) is responsible for phosphorylation of CMP, UMP, and deoxycytidine monophosphate (dCMP) and also pla

74 aker substrate for purified enzyme than CMP, UMP, or dCMP.

75 e, T-maze, and Y-maze tests; coadministering UMP further enhanced these increases.

76 from 137 to 680 fmol/min in those consuming UMP (P<0.05).

77 In contrast, UMP has no statistically significant impact on the modal

78 phosphate) versus their ribose-counterparts (UMP and 5-fluorouridine monophosphate), in a similar man

79 cement, with the participation of a covalent UMP-His 166-enzyme intermediate in the Escherichia coli

80 en atoms of the substrate in the crystalline UMP-enzyme and in the crystalline complex of H166G-GalT

81 88 reduces hydrogen bonding and destabilizes UMP-GALT.

82 Dietary UMP (0.5%, 1 week) also amplified the increase in ACh ca

83 tate based on the structure of D. discoideum UMP/CMP kinase aids to rationalize the substrate specifi

84 ted for the similar Dictyostelium discoideum UMP/CMP kinase reveals the conformational changes that o

85 al was obtained by the co-addition of either UMP or cytidine.

86 activation dominates the effects when either UMP or IMP is also bound.

87 synthase protein levels as well as elevated UMP synthase mRNA levels.

88 enzyme activity was correlated with elevated UMP synthase protein levels as well as elevated UMP synt

89 ate formation of a covalent uridylyl-enzyme (UMP enzyme).

90 ate formation of a covalent uridylyl-enzyme (UMP-enzyme).

91 ate formation of a covalent uridylyl-enzyme (UMP-enzyme).

92 haracteristic of known essential eubacterial UMP kinases.

93 inase has 50% identity with other eukaryotic UMP/CMP kinase proteins.

94 uterium exchange into enzyme-bound UMP and F-UMP of 1.2 x 10(-5) and 0.041 s(-1), respectively, so th

95 The binding of UMP and F-UMP to ScOMPDC results in 0.5 and 1.4 unit decreases, re

96 The binding of UMP and F-UMP to ScOMPDC results in a greater than 5 x 10(9)-fold

97 or k(cat)/K(m) for deuterium exchange into F-UMP gives the intrinsic second-order rate constant for e

98 UMP) and 5-fluorouridine 5'-monophosphate (F-UMP) catalyzed by yeast orotidine 5'-monophosphate decar

99 -fluorouracil (5-FU) and further to 5-fluoro-UMP, respectively.

100 levels; however, both basally and following UMP, these levels were much lower than those of uridine.

101 4) and 1.28 x 10(4) microM(-)(1) s(-)(1) for UMP and GMP, respectively.

102 rification scheme, in which the affinity for UMP was markedly reduced, CP rate curves showed no sigmo

103 monoesterase activity, with a preference for UMP > TMP > AMP >> CMP.

104 ion suggests that exogenous Up4A first forms UMP and ATP in the human colon and UDP and ADP in the mu

105 Using detailed market data from UMP, we analyze how features related to logistical chall

106 Furthermore, UMP has generated a greater benefit for farmers who prod

107 Furthermore, UMP/CMPK was able to phosphorylate all of the deoxycytid

108 mensional model of the Escherichia coli GALT-UMP protein crystal.

109 ing UDP-GalNAc, UDP-Glc, UDP-Gal, UDP-GalUA, UMP, UDP, and UTP.

110 ollowing: (i) all variants act as UDP-GlcNAc/UMP antiporters; (ii) conservative substitutions (E47D,

111 Here, the roles of GlnK and GlnK-UMP in A. vinelandii were studied to determine whether t

112 inhibited by NH(4)(+), suggesting that GlnK-UMP is required to signal adenylyltransferase/adenylyl-r

113 stringent with a preference of CMP > AMP >> UMP > GMP.

114 AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, and UTP) was examined by reversed-phase HPLC a

115 Human UMP synthase is a bifunctional protein containing two se

116 Human UMP/CMP kinase (cytidylate kinase; EC 2.7.4.14) is respo

117 Human UMP/CMP kinase plays a crucial role in supplying precurs

118 data suggest that the active sites of human UMP/CMP kinase for dCMP and for CMP cannot be identical.

119 dels for the phosphorylation action of human UMP/CMP kinase.

120 re of the substrate-free, open form of human UMP/CMP kinase.

121 a using recombinant or highly purified human UMP/CMP kinase showed that dCMP, as well as pyrimidine a

122 ction conditions for human recombinant human UMP/CMP kinase to phosphorylate dCMP and CMP (referred a

123 Thus, we cloned the human UMP/CMPK gene, expressed it in Escherichia coli, and pur

124 The reactivities of the mutants in UMP-dependent reductive inactivation by glucose are simi

125 s, we hypothesize that the covalent union in UMP synthase stabilizes the domains containing the respe

126 ases (TUTases) catalyze template-independent UMP addition to the 3' hydroxyl of RNA.

127 osteric activator ornithine or the inhibitor UMP despite the substantial and opposing effects these l

128 for V(max) (pK(a) values = 5.5, 9.0) and V/K(UMP)(-)(NeuAc) (pK(a)values = 6.2, 9.0).

129 er, as a non-heme, Fe(II)-dependent alpha-KG:UMP dioxygenase that produces uridine-5'-aldehyde to ini

130 eoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK);EC 2.7.4.14] plays a crucial role in the forma

131 Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK);EC 2.7.4.14] plays a crucial r

132 PRPP, which like UMP is a substrate for the uracil phosphoribosyltransfer

133 eaction in which the enzyme covalently links UMP to the hydroxyl group of tyrosine in the terminal pr

134 ermined to be K(m)(alpha-KG) = 7.5 muM, K(m)(UMP) = 14 muM, and k(cat) approximately 80 min(-1).

135 This assay measures UMP, the by-product of PGT reactions, in a sensitive and

136 When 10 or 100 microM UMP was added to reaction mixtures containing D39 membra

137 the C-6 protons of uridine 5'-monophosphate (UMP) and 5-fluorouridine 5'-monophosphate (F-UMP) cataly

138 o steps of de novo uridine 5'-monophosphate (UMP) biosynthesis are catalyzed by orotate phosphoribosy

139 the uridine source uridine-5'-monophosphate (UMP) can significantly increase levels of the phosphatid

140 hways whereby oral uridine-5'-monophosphate (UMP) increases membrane phosphatide synthesis in brains

141 phosphate (CP) and uridine 5'-monophosphate (UMP), showed pronounced sigmoidicity, each in the presen

142 a uridine source, uridine-5'-monophosphate (UMP), which increases brain levels of the rate-limiting

143 phosphate (OMP) to uridine 5'-monophosphate (UMP).

144 abidopsis thaliana uridine 5'-monophosphate (UMP)/cytidine 5'-monophosphate (CMP) kinase was isolated

145 Uridine monophosphate (UMP) kinase is a conserved enzyme that catalyzes the ATP

146 levels and decreases uridine monophosphate (UMP) levels, consistent with inhibition of UMP synthesis

147 hosphate (AMP) and 5'-uridine monophosphate (UMP) molecules confined in multi-lamellar phospholipid b

148 the conversion of 5'-uridine monophosphate (UMP) to UMP-NeuAc, which was found to be an inactive sia

149 nophosphate (IMP) and uridine monophosphate (UMP) were only 30% of those found in the wild-type cells

150 y annotating N-methyl-uridine monophosphate (UMP), lysomonogalactosyl-monopalmitin, N-methylalanine,

151 nversion of uracil to uridine monophosphate (UMP).

152 supplemental uridine (as its monophosphate, UMP; 0.5%) and choline (0.1%) via the diet, and docosahe

153 for the pyrimidine nucleoside monophosphates UMP and CMP.

154 In the absence of UMP, initial-rate curves for CP are hyperbolic.

155 10 and 220 nM in the presence and absence of UMP, respectively.

156 By contrast, the actions of UMP and IMP together must be explained with a competitiv

157 otide metabolism, namely the ODC activity of UMP synthase.

158 espect to uptake of orotic acid, affinity of UMP synthase for its substrates, or UMP synthase gene-co

159 inities that surpass the binding affinity of UMP.

160 anionic substituents at the 6-carbon atom of UMP.

161 s is initiated by the covalent attachment of UMP to the terminal protein VPg, yielding VPgpU and VPgp

162 The binding of UMP and F-UMP to ScOMPDC results in 0.5 and 1.4 unit dec

163 The binding of UMP and F-UMP to ScOMPDC results in a greater than 5 x 1

164 The binding of UMP to the allosteric domain inhibits the dimerization o

165 The concentration of UMP required for half-maximal effect was 2.5 microM.

166 The concentration of UMP required for half-maximal stimulation of binding of

167 n vitro, at physiological concentrations, of UMP synthase, the two isolated catalytic domains prepare

168 nd catalyzes the ATP-dependent conversion of UMP to UDP in vitro with properties characteristic of kn

169 id synthesis by catalyzing the conversion of UMP, CMP, and dCMP into their diphosphate form.

170 diastereomer mixture prepared by coupling of UMP-morpholidate with fluoromethylenebis(phosphonic acid

171 liganded conformation before dissociation of UMP and were inconsistent with several other kinetic mec

172 re more slowly incorporated, the excision of UMP is slow and inefficient, and modifications to the 2'

173 6-amino acid UMP/CMPK was the actual form of UMP/CMPK, rather than the 228-amino acid form as suggest

174 UDP-GlcNAc, with the resulting generation of UMP, a potent and competitive inhibitor of GnT-IX.

175 sphoenolpyruvate to the 3'-hydroxyl group of UMP.

176 this investigation were the imidazolides of UMP, xanthosine 5'-monophosphate, the bis-monophosphates

177 es method, we demonstrate that the impact of UMP on modal prices varies substantially across commodit

178 In particular, the implementation of UMP has yielded an average 5.1%, 3.6%, and 3.5% increase

179 oriI, templates processive incorporation of UMP into VPg by using a "slide-back" mechanism.

180 Through inhibition of UMP synthase, 5-AzaC also strongly induced expression of

181 (UMP) levels, consistent with inhibition of UMP synthesis at a step downstream of dihydroorotate deh

182 e HIV protease and the unfolding kinetics of UMP/CMP kinase, a globular protein from Dictyostelium di

183 lines), showed an unexpected, high level of UMP synthase and was therefore analyzed in detail.

184 of these cell lines showed reduced levels of UMP synthase.

185 s 2 (HRV2) catalyzes the covalent linkage of UMP to the terminal protein (VPg) using poly(A) as a tem

186 PV) RNA synthesis is the covalent linkage of UMP to the terminal protein VPg.

187 The mechanism of UMP attachment at the active site of the transferase is

188 Whereas the enzymatic mechanism of UMP kinase (UK) is well-characterized, the molecular bas

189 replication is initiated when a molecule of UMP is covalently linked to the hydroxyl group of a tyro

190 replication is initiated when a molecule of UMP is covalently linked to the hydroxyl group of a tyro

191 ins prepared by site-directed mutagenesis of UMP synthase, and the yeast ODCase.

192 hydrogen bonds to the phosphoryl oxygens of UMP with lengths of 2.52 and 2.82 A.

193 The role of UMP/CMPK for the phosphorylation of nucleoside analogues

194 A series of UMP-NeuAc radioisotopomers were prepared by chemical dea

195 ckettsial CTP appears to be the transport of UMP followed by its phosphorylation and the amination of

196 d at $21.7 billion (USD) have been traded on UMP.

197 Treatment of ([PPN](2)[1]) with AMP or UMP yields nucleoside-monophosphate tetrametaphosphates

198 tes, were much poorer substrates than CMP or UMP for this enzyme.

199 mented with uracil, uridine, deoxyuridine or UMP.

200 and in each major phosphatide; giving DHA or UMP (plus choline) produced smaller increases in some of

201 AE1 is not inhibited by UDP-Glc, UDP-Gal, or UMP.

202 upling between MgADP and either ornithine or UMP.

203 inity of UMP synthase for its substrates, or UMP synthase gene-copy number.

204 These results show that oral UMP, a uridine source, enhances the synthesis of CDP-cho

205 All three allosteric ligands, ornithine, UMP, and IMP, act by modifying the affinity of CPS for t

206 habeta)2 dimer in the presence of ornithine, UMP, or IMP.

207 The amounts of [(32)P]UMP incorporated into VPgpUpU(OH) and negative-strand RN

208 the ubiquitin-mediated proteolysis pathway (UMP).

209 case, one would expect uridine 5'-phosphate (UMP) derivatives with bulky anionic substituents at C6 t

210 oy the communication between the PII and PII-UMP binding sites and the AT and AR active sites.

211 educed by PII-UMP, whereas glutamine and PII-UMP competed for the enzyme.

212 gulation of ATase by glutamine, PII, and PII-UMP consistent with all data is presented.

213 we observed that essentially all PII and PII-UMP interactions were influenced by ADP.

214 and PII-UMP, these domains bound PII and PII-UMP significantly better when linked to the central regi

215 he binding of these effectors to PII and PII-UMP was characterized.

216 ent with the protein activators (PII and PII-UMP) binding to the enzyme domain with the opposing acti

217 clusively through its binding to PII and PII-UMP, did not alter the binding of PII or PII-UMP to the

218 polypeptides were indifferent to PII and PII-UMP, or their ATase activity was inhibited by either PII

219 domains of ATase bound poorly to PII and PII-UMP, these domains bound PII and PII-UMP significantly b

220 o contain two distinct sites for PII and PII-UMP.

221 ultiple sites for the binding of PII and PII-UMP.

222 which did so by binding only to PII and PII-UMP.

223 sensors of 2-ketoglutarate were PII and PII-UMP.

224 ha-ketoglutarate, which binds to PII and PII-UMP.

225 and pyrophosphate (PPi) released before PII-UMP.

226 g (AR) activity of ATase is activated by PII-UMP and inhibited by unmodified PII and by glutamine.

227 l-removing (AR) reaction is activated by PII-UMP and is inhibited by glutamine and by PII.

228 ed by glutamine and its level reduced by PII-UMP, whereas glutamine and PII-UMP competed for the enzy

229 transduction protein and is inhibited by PII-UMP.

230 UMP, did not alter the binding of PII or PII-UMP to the enzyme.

231 on or inhibition of the enzyme by PII or PII-UMP.

232 le not eliminating the binding of PII or PII-UMP.

233 activity was inhibited by either PII or PII-UMP.

234 Our results were consistent with PII, PII-UMP, and glutamine shifting the enzyme among at least si

235 The PII, PII-UMP, and glutamine sites were in communication so that t

236 hibited by the uridylylated form of PII, PII-UMP.

237 The deduced amino acid sequence of the plant UMP/CMP kinase has 50% identity with other eukaryotic UM

238 Following proteolytic digestion, the plant UMP/CMP kinase was purified and analyzed for its structu

239 In this study, we show that the sole plastid UMP kinase (PUMPKIN) in Arabidopsis (Arabidopsis thalian

240 plementation of the Unified Market Platform (UMP)-on market prices and farmers' profitability.

241 Addition of the PyrR regulatory protein plus UMP led to greatly increased termination.

242 Transcriptional attenuation by PyrR plus UMP was also demonstrated in vitro with templates from t

243 ate, uracil (2.2 A resolution), its product, UMP (2.5 A resolution), and the prodrug, 5-fluorouracil

244 e of the LdUMPS in complex with its product, UMP.

245 were measured using mixtures of radiolabeled UMP-NeuAc's as the donor substrate and N-acetyllactosami

246 In the present study, adult gerbils received UMP (1 mmol/kg), a constituent of human breast milk and

247 similar reaction of the structurally related UMP kinase.

248 reiterative transcription (i.e., repetitive UMP addition) within the 8-bp T.

249 5-fold tighter in the presence of saturating UMP or UDP and 150- fold tighter with saturating UTP, su

250 for the linkage of both the first and second UMPs to VPg, 3) VPgpUpU is synthesized by a "slide-back"

251 The smallest UMP dose that significantly increased brain CDP-choline

252 f the novel sugar-nucleotide donor substrate UMP-NeuAc.

253 anished with use of the slow donor substrate UMP-NeuAc.

254 In the absence of the prime substrate UMP, LipL is able to catalyze oxidative decarboxylation

255 model, consistent with previous reports that UMP and IMP bind to the same site.

256 The UMP pyrimidine ring stacks against the uracil base of th

257 interactions between the active site and the UMP group of E-His(166)-UMP provide little or no stabili

258 kage is a phosphoramidate formed between the UMP moiety and the His 166 N(epsilon)(2) of GalT, with H

259 ption whereby termination is governed by the UMP-dependent binding of PyrR to pyr RNA and provide pur

260 Young rats eating the UMP-containing diet exhibited similar increases in basal

261 onclusion derives from studies employing the UMP synthesis inhibitor N-phosphonacetyl-L-aspartate (PA

262 None of the variants formed the UMP-enzyme in detectable amounts upon reaction with UDP-

263 ferase (OMP synthase, EC 2.4.2.10) forms the UMP precursor orotidine 5'-monophophate (OMP) from orota

264 glucose-1-phosphate (glu-1-P), and forms the UMP-GALT intermediate.

265 However, the UMP . GCP complex was 4.8 degrees less closed than the g

266 n bonding with the phosphoryl oxygens of the UMP moiety, which is bonded to His 166 in the intermedia

267 (31)P NMR studies of the UMP product generated from UDP-2,3-diacylglucosamine in

268 (ScOMPDC) catalyze the exchange of H6 of the UMP product with solvent deuterium allows an estimate of

269 tidylate decarboxylase (ODC) activity of the UMP synthase enzyme complex that catalyzes an early even

270 ound that AHR mediates the expression of the UMP-generating enzymes dihydroorotate dehydrogenase (qui

271 n authentic and well conserved member of the UMP/CMP kinase group while EhAK is the most divergent me

272 hat glutamine at position 188 stabilizes the UMP-GALT intermediate through hydrogen bonding and enabl

273 Kinetic analysis revealed that the UMP/CMP kinase preferentially uses ATP (Michaelis consta

274 hat delivers a solvent-derived proton to the UMP product; the active site of KGPDC contains a homolog

275 active sites underwent uridylylation to the UMP-enzyme, similar to wild-type GalT, upon reaction wit

276 the presence of SII, incorporation of 4-thio-UMP into RNA, and irradiation and was sensitive to treat

277 without a significant price increase due to UMP.

278 e EhUK was more similar (48-49% identity) to UMP/CMP kinases.

279 al ion-independent decarboxylation of OMP to UMP and CO(2).

280 he enzyme-catalyzed transformation of OMP to UMP may have less of an energetic impact than commonly t

281 led to uracil and then phosphoribosylated to UMP in the parasite by LdUPRT.

282 atives by spontaneous addition of sulfite to UMP and to OMP.

283 ENTPD5 hydrolyzes UDP to UMP to promote protein N-glycosylation and folding in ER

284 lucosyltransferase inhibitory product UDP to UMP, and that the latter product then exits the lumen of

285 ation of an ER enzyme that hydrolyzes UDP to UMP.

286 version of 5'-uridine monophosphate (UMP) to UMP-NeuAc, which was found to be an inactive sialyl dono

287 sted the kinetic competence of the wild-type UMP-enzyme.

288 The unstable UMP-GALT allows single displacement of glu-1-P with rele

289 examined, but no catalysis of [(14)C]uracil-UMP and [(32)P]PP(i)-phosphoribosylpyrophosphate exchang

290 These KIEs observed using UMP-NeuAc are much larger than those previously measured

291 When UMP or UTP is bound to the PyrR regulatory protein, it b

292 TP (Michaelis constant [Km] = 29 microM when UMP is the other substrate and Km = 292 microM when CMP

293 hine and IMP function as activators, whereas UMP is an inhibitor.

294 oline (ACh) synthesis, we determined whether UMP administration also affects ACh levels in striatum a

295 CP rate curves showed no sigmoidicity, while UMP rate curves had sigmoidicity exaggerated by a low ma

296 formation of an (alphabeta)4 tetramer while UMP favors the formation of an (alphabeta)2 dimer.

297 pses, particularly when co-administered with UMP.

298 uents at C6 to be bound weakly compared with UMP, which is unsubstituted at C6.

299 0-A resolution structure of the complex with UMP/GCP and a 2.8-A resolution structure of the complex

300 nor are they removed from an FAD resin with UMP (which shares a pattern of hydrogen bond donors and