ライフサイエンスコーパス: USV

1 USV trait clustering predicted adult social deficits, hi

2 USVs and locomotion were measured for 6 min, with antino

3 urately classified 86% of the USVs out of 11 USV categories.

4 In a data set of >4000 USVs emitted by mice, VocalMat detected over 98% of manu

5 Here, we present DeepSqueak, a USV detection and analysis software suite that can perfo

6 DeepSqueak allows USV recording and analysis to be added easily to existin

7 egrees of genetic dominance and that cry and USV features can be uncoupled in second-generation hybri

8 ocked or severely impaired both freezing and USV elicited as CRs but had no effect on either behavior

9 hock paired training, postshock freezing and USV responses were significantly impaired in BLA-lesione

10 nance, or expression, then both freezing and USV should be blocked or impaired when elicited as CRs d

11 both postshock and conditioned freezing and USV.

12 ed females promotes social investigation and USV production but does not recapitulate the effects of

13 We investigated whether USV(+) and USV(-) mounting use the same or distinct hypothalamic ne

14 terns of neuronal activity during USV(+) and USV(-) mounting, and the type of mounting could be decod

15 ences in the acoustic structure of cries and USVs, we find that variation in vocalization rate, durat

16 male mice, and that both PAG-USV neurons and USVs can be switched on by their inputs from the preopti

17 nt studies using microphone arrays to assign USVs to individual signalers report that females produce

18 loying methods that can unambiguously assign USVs to individual signalers, regardless of inter-mouse

19 Reliably attributing USVs to their emitter during close interactions has emer

20 ugh ablation of these PAG-USV neurons blocks USV production in both males and females, these neurons

21 activation can determine the lengths of both USV syllables and concurrent expiration periods, with th

22 significantly impaired conditioning to both USV conditional stimuli and to the training context but

23 ed fear in rats of both sexes as assessed by USVs.

24 e present study is the first to characterize USV responses to the same aversive event throughout deve

25 e by using acoustic analysis to characterize USVs and a principled supervised learning setup.

26 ion and machine learning methods to classify USVs into distinct categories.

27 The factors that regulate female courtship USV production are poorly understood.

28 re, we tested the idea that female courtship USV production is regulated by estrous state.

29 studies have overestimated female courtship USV production.

30 mice produce substantial rates of courtship USVs only when interacting with vocal male partners or t

31 g thought that only males produced courtship USVs, but recent studies using microphone arrays to assi

32 produce a portion (5-18%) of total courtship USVs.

33 This study attempted to deconstruct USVs into simpler stimulus features that cause fear cond

34 -day-old pups; higher doses of MOR decreased USV but produced motor deficits as well.

35 tic measures can be used by labs to describe USVs and compare data between groups, and provide insigh

36 d differential geometry approaches to detect USVs in audio files, eliminating the need for user-defin

37 r understanding of the function of different USVs in juvenile female and male rats.

38 Thus, pups' differential USV responses to contact with passive adults in isolatio

39 ations (USVs) and newborn pups emit distress USVs when separated from their dam, thereby facilitating

40 istinct patterns of neuronal activity during USV(+) and USV(-) mounting, and the type of mounting cou

41 the amygdala and PAG is sufficient to elicit USV production in socially isolated male mice.

42 r, the conditions classically used to elicit USV vary greatly with the animal's age (isolation from t

43 return by itself is not sufficient to elicit USV.

44 uperior vena cava or carotid artery elicited USV from pups in their home cage.

45 the hypothalamus (POA(PAG) neurons) elicited USV production in the absence of social cues.

46 nervating the parabrachial nucleus, elicited USVs in both male and female mice.

47 g) attenuated BAT metabolism while enhancing USV production, and norepinephrine (NE, 800 microg/kg) e

48 gainst the two leading hypotheses explaining USV production: superficial vocal fold vibrations [2], a

49 To facilitate the detection of female USVs, we paired females with males that were muted for U

50 FM and flat USVs are produced by both sexes during tickling, but it

51 darting and hand approaches) and FM and flat USVs emitted during the testing session were quantified

52 each rat, with the frequency of FM and flat USVs made in anticipation of, and during, each behaviour

53 ay behaviours were associated with more flat USVs than in males (before and during; p < 0.001), ir

54 e may be sex differences in the role of flat USVs during play.

55 The higher call rate of flat USVs paired with play behaviour in females suggests that

56 aviours in a sex-specific way, and that flat USVs are associated with non-play activities.

57 FM USVs were paired with hopping and darting (before and du

58 We tested the hypotheses that FM USVs are associated with tickle-induced play behaviours

59 to controls (both sexes) suggesting that FM USVs are linked with play behaviour.

60 (PAG-USV neurons) are an obligatory gate for USV production in both male and female mice, and that bo

61 r venous return is a necessary mechanism for USV production, 5% dextrose in water or blood was infuse

62 aired females with males that were muted for USV production via caspase-mediated ablation of midbrain

63 on of midbrain neurons that are required for USV production.

64 cending circuit necessary and sufficient for USV production while also demonstrating the communicativ

65 MUPET thus serves as a new tool for USV repertoire analyses, with the capability to be adapt

66 , where the drive and courtship function for USVs are better understood.

67 es during training, as measured by freezing, USV, and defecation.

68 that the expression of conditioned freezing, USV, defecation, and analgesia were significantly impair

69 Rabies tracing from USV-responsive neurons reveals extensive subcortical and

70 Although play deprivation enhanced high USVs, an arousing but aversive stimulus (bright light) r

71 characterized eliciting conditions for high USVs.

72 n together, these findings suggest that high USVs may index an appetitive motivation to play in juven

73 This findings suggested that high USVs were linked to a motivational state rather than spe

74 igh-frequency ultrasonic vocalizations (high USVs, approximately 55 kHz) during rough-and-tumble play

75 o three groups based on PD10 USVs, with high USVs associated with reduced PD100 sociability.

76 , that these inhibitory inputs are active in USV-promoting social contexts, and that optogenetic acti

77 perthermia, and adults showed alterations in USV observed as aftereffects of intoxication, despite gr

78 This indicates a specific deficit in USV calling rate in Fmr1 KO mice.

79 r dyads, without using the predictability in USV sequences.

80 We hypothesize that MS results in USV emission patterns reflective of a greater stress res

81 2 by siRNA eliminated the sex differences in USVs and altered the order of pup retrieval.

82 in fine sensorimotor function that includes USV production and complexity.

83 After removal of the dam, pups increased USV rates over baseline (potentiation).

84 stimulation produced analgesia and increased USVs and locomotion.

85 potentiated by NPA, whereas U-50,488-induced USVs were attenuated by both DA agonists.

86 should have inverse effects on cold-induced USVs.

87 Next, we questioned if these infant USV were also emitted in a more naturalistic context by

88 he hypothesis that BAT metabolism influences USVs during cold challenge by affecting cardiac rate and

89 ineered to allow non-experts easy entry into USV detection and analysis yet is flexible and adaptable

90 hort-term isolation on social investigation, USV production, and mounting.

91 ned to one of three cues: a multicall 19-kHz USV, a 19-kHz discontinuous tone, and a 19-kHz continuou

92 d in rat PR by a 22 kHz tone cue or a 22 kHz USV cue.

93 This treatment enhanced 40-kHz USV while leaving 66-kHz USV unchanged suggesting that t

94 ition, but led to increased prosocial 50-kHz USV emission rates and enhanced social approach behavior

95 Juvenile rats emit distinctive 50-kHz USV subtypes.

96 ent enhanced 40-kHz USV while leaving 66-kHz USV unchanged suggesting that the use of USV goes far be

97 duces social approach behavior, while 22 kHz USVs lead to freezing behavior.

98 ns, rats produce 50 kHz USVs, whereas 22 kHz USVs occur in aversive situations.

99 (USV) conditional stimuli (10 sec of "22 kHz USVs").

100 in random order: (1) 50 kHz USVs, (2) 22 kHz USVs, (3) time- and amplitude-matched white noise, and (

101 n patterns, with 50 kHz USVs, but not 22 kHz USVs, activating neurons in the nucleus accumbens (NAcc)

102 ypothesized that 50 kHz USVs, but not 22 kHz USVs, elicit NAcc dopamine release.

103 -social 50 kHz USVs, but not alarming 22 kHz USVs.

104 d in a nor-BNI reversible increase in 22-kHz USVs (mimicking an alcohol-dependent state).

105 onism blocked the increased number of 22-kHz USVs observed during acute alcohol withdrawal and a KOR

106 Only presentation of 50 kHz USVs induced phasic dopamine release and elicited approa

107 Reception of 50 kHz USVs induces social approach behavior, while 22 kHz USVs

108 trasonic speaker in random order: (1) 50 kHz USVs, (2) 22 kHz USVs, (3) time- and amplitude-matched w

109 tinct brain activation patterns, with 50 kHz USVs, but not 22 kHz USVs, activating neurons in the nuc

110 Therefore, we hypothesized that 50 kHz USVs, but not 22 kHz USVs, elicit NAcc dopamine release.

111 c dopamine release encodes pro-social 50 kHz USVs, but not alarming 22 kHz USVs.

112 n appetitive situations, rats produce 50 kHz USVs, whereas 22 kHz USVs occur in aversive situations.

113 Frequency-modulated (FM) 50-kHz USVs are thought to be associated with positive affect a

114 iments 1 and 2, rats showed increased 50-kHz USVs before receiving experimenter-delivered ventral teg

115 MPH microinjections selectively evoke 50-kHz USVs in rats, supporting the notion that dopamine elevat

116 3 and 4, rats increased their rate of 50-kHz USVs in response to cues that predicted the opportunity

117 ngs support the hypothesis that short 50-kHz USVs may selectively index a state of reward anticipatio

118 ociated with positive affect and flat 50-kHz USVs with social communication.

119 daily 1-hr feeding sessions increased 50-kHz USVs, whereas a cue that predicted footshock decreased 5

120 d robust, dose-dependent increases in 50-kHz USVs, which could not be accounted for by concomitant in

121 n LA without significant increases in 50-kHz USVs.

122 ue that predicted footshock decreased 50-kHz USVs.

123 pe of ESB evoked 20-kHz, rather than 50-kHz, USVs.

124 calMat detected over 98% of manually labeled USVs and accurately classified 86% of the USVs out of 11

125 on (~3.4-4.8 mm, 91% assigned) in localizing USVs, ~3x better than other systems, approaching the phy

126 apturing the observed predictability in male USVs in different contexts of social interaction with fe

127 nstrating the communicative salience of male USVs.

128 teract with kappa opioid systems to modulate USVs, antinociception, and locomotion in preweanling rat

129 ted male rat pups emitted substantially more USV calls and these were characterized by a significantl

130 n algorithm previously used to analyse mouse USVs and birdsongs.

131 of predictive sequences and introduces mouse USVs as a promising model to study context-dependent spe

132 d EMG responses and displayed practically no USV behavior.

133 nditioned EMG responses but exhibited normal USV behavior, whereas animals with lesions to the amygda

134 n juvenile and adult rats, a single class of USV is observed with an age-dependent main frequency and

135 Phenotypic clustering of USV and cognitive traits was performed, and high-through

136 s to analyze the probability distribution of USV classification among different experimental groups,

137 ontext-specific natural sequences (NSeqs) of USV syllables capturing the observed predictability in m

138 Studies using playback of USV sequences have used randomly selected sequences from

139 d that social context shapes a wide range of USV amplitudes and bout durations.

140 e mother, accounting for the greater rate of USV production during the second isolation period.

141 of venous return did not affect the rate of USV.

142 All pups reduced rates of USV in contact with anesthetized adults.

143 activated, elicit the complete repertoire of USV syllables emitted during natural courtship.

144 Chemogenetic silencing of USV-responsive neurons in TeA impairs auditory-driven ma

145 nalyses characterizing the bout structure of USV production indicated that the average bout size (i.e

146 kHz USV unchanged suggesting that the use of USV goes far beyond a signal studied in terms of amount

147 The average amplitude of USVs was also greater following maternal reunion.

148 des data-driven, high-throughput analyses of USVs.

149 Recording and analysis of USVs has broad utility during diverse behavioral tests a

150 ated, accurate, and quantitative analysis of USVs without the need for user inputs, opening the oppor

151 Consistent with prior studies, the number of USVs emitted was significantly increased in the period f

152 d that the average bout size (i.e. number of USVs/bout) was increased severalfold following the reuni

153 Neural representation of USVs in the mouse primary auditory cortex (Au1) and its

154 trajectories that are distinct from those of USVs.

155 Offspring USVs influenced maternal care behaviours, but postnatal

156 t not postnatal rearing, increased offspring USVs, maternal care behaviours, and impaired adult cogni

157 In 10-day-olds, USV has been shown to be reduced by either the administr

158 f NAcc amphetamine (AMPH) microinjections on USVs.

159 One USV CS was a continuous sequence of eight calls, and the

160 but does not affect social investigation or USV production.

161 ut does not decrease social investigation or USV production.

162 nt firing changes in response to the tone or USV conditional stimulus (CS) after it had been paired s

163 ey (PAG) inhibition, enabling excitatory PAG USV-gating neurons to trigger vocalizations.

164 Conversely, activating PAG-USV neurons selectively triggered USV production, even i

165 /M-PAG) neurons, along with POA(PAG) and PAG-USV neurons, form a nested hierarchical circuit in which

166 both male and female mice, and that both PAG-USV neurons and USVs can be switched on by their inputs

167 sonic vocalizations (USVs) in mice (i.e. PAG-USV neurons).

168 ons in the midbrain periaqueductal gray (PAG-USV neurons) are an obligatory gate for USV production i

169 PAG interneurons, which in turn inhibit PAG-USV neurons, whereas Amg(C/M-PAG) neurons directly inhib

170 as Amg(C/M-PAG) neurons directly inhibit PAG-USV neurons.

171 Genetic silencing of PAG-USV neurons rendered males unable to produce USVs and im

172 bined with axonal tracing indicates that PAG-USV neurons gate downstream vocal-patterning circuits.

173 We find that, although ablation of these PAG-USV neurons blocks USV production in both males and fema

174 ng clustered into three groups based on PD10 USVs, with high USVs associated with reduced PD100 socia

175 Pups reared with aunts potentiated USV after contact with adult females but suppressed USV

176 Pups reared with sires potentiated USV after contact with sires or strange males.

177 and previously isolated female mice produce USVs during social encounters with novel females.

178 USV neurons rendered males unable to produce USVs and impaired their ability to attract females.

179 ously into isolated pups that were producing USV.

180 he recovery of the inhibition clamp prolongs USV bouts.

181 express ESR1 (VMHvl(ESR1) neurons) promoted USV(-) mounting, and inhibited the USVs evoked by female

182 (MPOA(ESR1 VGAT) neurons) robustly promoted USV(+) mounting, and converted male-directed attack to m

183 increased social dominance, and reduced pup USV.

184 Pups' USV rates after the adult's removal depended on their re

185 performance, the alterations in their pups' USVs and maternal potentiation do not appear to result f

186 oftware suite that can perform human quality USV detection and classification automatically, rapidly,

187 In contrast, infant rat USV were split into two classes with specific relationsh

188 nhanced proximal orientation toward recorded USVs, however, if a silent pup was positioned below the

189 When presented with recorded USVs, mothers of 6- to 8-day-old pups and nulliparous vi

190 kg dose of MOR is less effective in reducing USV in 3- and 7-day-olds; calling rates declined by no m

191 These data suggest that mating-related USVs are robust and relevant biomarkers of FXS, and that

192 tion cortex (TeA) in mothers exhibits robust USV responses.

193 ir social and affective significance, rodent USVs are increasingly used as a behavioral measure in ne

194 reared with castrated males did not suppress USV after contact with castrates but did after contact w

195 cues or during social contexts that suppress USV production in male and female mice.

196 er contact with adult females but suppressed USV after contact with males.

197 Amg(C/M-PAG) neurons) transiently suppressed USV production without disrupting non-vocal social behav

198 re rapid approach by Peromyscus mothers than USVs, suggesting a role for cries in eliciting parental

199 adult male mice during mating, we show that USV calling rate (number of calls/second) is reduced in

200 Despite strong evidence that USVs serve an array of communicative functions, technica

201 ic and contextual differences, we found that USVs and songs do not arise from parallel pathways.

202 etween single-unit responses elicited by the USV cue and those elicited by the tone cue.

203 he units (14 of 123 units) recorded from the USV-conditioned group displayed a precisely timed increa

204 n/off pattern of the individual calls in the USV, but it lacked the characteristic frequency modulati

205 firing was approximately 30 ms longer in the USV-conditioned group than in the tone-conditioned group

206 The presence of a companion also lowered the USV of 3- and 7-day-olds by a lesser amount (55-57%) tha

207 early anxiety state, and potentiation of the USV response after brief maternal encounters is a newly

208 Here, we show that the USV-suppressing Amg(C/M-PAG) neurons are strongly activa

209 oxic PR lesions impaired conditioning to the USV, the discontinuous tone, and the training context.

210 This US-timed response was unique to the USV-conditioned group.

211 on of conditioned EMG responses, whereas the USV behavior was unaffected.

212 promoted USV(-) mounting, and inhibited the USVs evoked by female urine.

213 ed USVs and accurately classified 86% of the USVs out of 11 USV categories.

214 re presented with mild foot-shocks and their USV frequency, duration, and relationship with respirati

215 To detect and classify these USVs, here we describe VocalMat.

216 th single-neuron and population responses to USVs in TeA, improving discriminability of pup calls in

217 We report that total USVs recorded during interactions between group-housed B

218 vating PAG-USV neurons selectively triggered USV production, even in the absence of any female cues.

219 The two USV conditional stimuli were multi-call segments that we

220 Using USV data from a large mouse genetic reference panel and

221 aviours, offspring ultrasonic vocalisations (USV), and cognition were assessed in both sexes.

222 oduction of 50-kHz ultrasonic vocalisations (USVs), although this has mostly been investigated in mal

223 hy (EMG) and 22 kHz ultrasonic vocalization (USV) activities were measured concurrently from the same

224 freezing and 22 kHz ultrasonic vocalization (USV) as dependent measures of fear in rats.

225 d two different rat ultrasonic vocalization (USV) conditional stimuli (10 sec of "22 kHz USVs").

226 tioning to a 22 kHz ultrasonic vocalization (USV) cue.

227 identified impaired ultrasonic vocalization (USV) in a severe SMA mouse model-a proxy for early-devel

228 f isolation-induced ultrasonic vocalization (USV) of infant rats (Rattus norvegicus) were measured on

229 The ultrasonic vocalization (USV) response of the isolated infant rat is a promising

230 f social rearing on ultrasonic vocalization (USV) responses of 11- to 12-day-old rat (Rattus norvegic

231 g analgesia, 22 kHz ultrasonic vocalization (USV), defecation, and freezing.

232 rtle reactivity and ultrasonic vocalization (USV).

233 freezing and 22 kHz ultrasonic vocalization (USV)] elicited under three conditions (during context co

234 mice, we recorded ultrasonic vocalizations (USV) and found that although both wild-type (WT) and het

235 These ultrasonic vocalizations (USV) are typically described and evaluated using expert

236 f prosocial 50-kHz ultrasonic vocalizations (USV) paralleled by a lack of social approach in response

237 By recording ultrasonic vocalizations (USV) produced by adult male mice during mating, we show

238 Ultrasonic vocalizations (USV) were measured at PND12 during two, 3-minute isolati

239 ut life, rats emit ultrasonic vocalizations (USV) when confronted with an aversive situation.

240 produce ultrasonic courtship vocalizations (USVs).

241 s: soft, variable, ultrasonic vocalizations (USVs) ancestral to rodents, used for short-range communi

242 ts communicate via ultrasonic vocalizations (USVs) and newborn pups emit distress USVs when separated

243 Rodent ultrasonic vocalizations (USVs) are a vital tool for linking gene mutations to beh

244 Here, we study how ultrasonic vocalizations (USVs) are represented in the brain of mothers.

245 fields using mouse ultrasonic vocalizations (USVs) as a natural and ethologically relevant stimulus a

246 increase in 22-kHz ultrasonic vocalizations (USVs) associated with alcohol withdrawal and KOR activat

247 ed rates of 50-kHz ultrasonic vocalizations (USVs) before receiving social and pharmacological reward

248 ar conditioning to ultrasonic vocalizations (USVs) but have no effect on conditioning to continuous t

249 Mouse ultrasonic vocalizations (USVs) contain predictable sequential structures like bir

250 Rat pups emit ultrasonic vocalizations (USVs) during cold challenge.

251 duce high rates of ultrasonic vocalizations (USVs) during courtship interactions with females.

252 Male mice produce ultrasonic vocalizations (USVs) during courtship to facilitate mating, and previou

253 p that is emitting ultrasonic vocalizations (USVs) far more than do virgin females.

254 Ultrasonic vocalizations (USVs) fulfill an important role in communication and nav

255 the production of ultrasonic vocalizations (USVs) in mice (i.e. PAG-USV neurons).

256 the production of ultrasonic vocalizations (USVs) in mice.

257 proposed that all ultrasonic vocalizations (USVs) in young rats are by-products of a cardiovascular

258 adult rats, 50-kHz ultrasonic vocalizations (USVs) index a state characterized by high arousal and ex

259 Rats emit ultrasonic vocalizations (USVs) that are thought to serve as situation-dependent a

260 Mice emit ultrasonic vocalizations (USVs) that communicate socially relevant information.

261 Ultrasonic vocalizations (USVs) were altered in both sexes.

262 Rat pups emit ultrasonic vocalizations (USVs) when isolated in a novel environment.

263 ence or absence of ultrasonic vocalizations (USVs)(2-4), respectively.

264 t innate courtship ultrasonic vocalizations (USVs)(4,5).

265 estigation, social ultrasonic vocalizations (USVs), and mounting during same-sex interactions that fo

266 pa opioid-mediated ultrasonic vocalizations (USVs), antinociception, and locomotion in young rats.

267 Like ultrasonic vocalizations (USVs), DA signals were modulated by delivery of reward t

268 Ultrasonic vocalizations (USVs), freezing, and corticosterone responses were quant

269 d Mus pups produce ultrasonic vocalizations (USVs), Peromyscus pups also produce a second call type w

270 easures to compare ultrasonic vocalizations (USVs), which limits the ability to address repertoire co

271 nd eliciting mouse ultrasonic vocalizations (USVs).

272 rich repertoire of ultrasonic vocalizations (USVs).

273 cited freezing and ultrasonic vocalizations (USVs; 22 kHz) were measured after 10 tone-shock training

274 elicited under all three conditions, whereas USV should be spared.

275 increased across training sessions, whereas USV responses were initially robust but decreased across

276 in postnatal days one through nine, whereas USVs are primarily made after day 9.

277 ticular play-related behaviours, and whether USV subtypes are used in a sexually dimorphic manner dur

278 We investigated whether USV(+) and USV(-) mounting use the same or distinct hypo

279 us return was decreased in infant rats while USV and cardiovascular measures were monitored.

280 verted male-directed attack to mounting with USVs.