ライフサイエンスコーパス: V. fischeri

1 V. fischeri ADP-r has no significant homology (DNA or am

2 V. fischeri colonizes the crypts of a host organ that is

3 V. fischeri culture media have lower osmolarities than a

4 V. fischeri has six flagellin genes that are uniquely ar

5 V. fischeri hnoX encodes a heme NO/oxygen-binding (H-NOX

6 V. fischeri qsrP and ribB mutants exhibited no distinct

7 llowing first exposure, only approximately 5 V. fischeri cells aggregated on the organ surface.

8 A V. fischeri mutant defective in chitin catabolism was ab

9 A V. fischeri mutant unable to produce PepN is significant

10 nization of juvenile E. scolopes; however, a V. fischeri strain lacking TMAO reductase activity displ

11 d the molecular biological construction of a V. fischeri halovibrin null strain.

12 lysaccharide, however, little is known about V. fischeri biofilm matrix components.

13 These updates further advance V. fischeri as an important model for understanding inte

14 ing conditions, aerobactin production allows V. fischeri ES114 to competitively exclude Vibrio harvey

15 Also, V. fischeri strains were markedly more successful than V

16 Although V. fischeri encodes two putative large adhesins, LapI (n

17 with the luciferases from P. leiognathi and V. fischeri.

18 ntain a mixed population of Vibrio logei and V. fischeri, with V. logei comprising between 63 and 100

19 Specifically, V. natriegens and V. fischeri moved parallel to the wall and P. putida and

20 Another V. fischeri gene, ainS, directs the synthesis of N-octan

21 cating alterations in membrane physiology as V. fischeri initiates its symbiotic program.

22 RNA-Seq dataset representing host-associated V. fischeri cells from colonized juvenile E. scolopes, a

23 ame the apparent restriction barrier between V. fischeri and Escherichia coli.

24 portant insight into the interaction between V. fischeri and E. scolopes.

25 s an important role in the symbiosis between V. fischeri and its squid host.

26 nK, which suppresses cellular aggregation by V. fischeri prior to light organ entry.

27 ways important for symbiotic colonization by V. fischeri and establishes a paradigm for evaluating tw

28 s biofilm formation and host colonization by V. fischeri via its impact on transcription of the symbi

29 tA promotes successful squid colonization by V. fischeri, supporting its potential role in initiation

30 tivated to promote symbiotic colonization by V. fischeri.

31 etry demonstrated that oxygen consumption by V. fischeri CydAB quinol oxidase is inhibited by NO trea

32 important insights into biofilm dispersal by V. fischeri.

33 ween OMV production and biofilm formation by V. fischeri.

34 m for the inhibition of biofilm formation by V. fischeri.

35 environment to regulate biofilm formation by V. fischeri.

36 protein necessary for biofilm maturation by V. fischeri and, based on the conservation of bmp, poten

37 ed whether the net release of PG monomers by V. fischeri resulted from lytic transglycosylase activit

38 lete understanding of the matrix produced by V. fischeri to enhance cell-cell interactions and promot

39 for promoting colonization of E. scolopes by V. fischeri.

40 During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO)

41 In contrast, V. fischeri ES114 mutants incapable of aerobactin produc

42 genes) was absent in over 97% of these dark V. fischeri strains.

43 These 'dark' V. fischeri strains remained non-bioluminescent even aft

44 We used a recently developed V. fischeri microarray to identify genes that are contro

45 high inocula demonstrated that environmental V. fischeri cells aggregate during a 3 h period in host-

46 4.3-Mbp genome sequence represents the first V. fischeri genome from an S. robusta symbiont and the f

47 d from the marine bacterium Vibrio fischeri (V. fischeri ADP-r) is described.

48 irectly and in real time, approximately five V. fischeri cells aggregate along the mucociliary membra

49 ion factor CysB is shown to be necessary for V. fischeri both to grow on several sulfur sources in vi

50 These results indicate a role for V. fischeri AOX in aerobic respiration during NO stress.

51 In a screen for V. fischeri colonization mutants, we identified a strain

52 nsible for this phenomenon, the lipid A from V. fischeri ES114 LPS was isolated and characterized by

53 Lipid A from V. fischeri, E. coli, or S. flexneri induced apoptosis.

54 A 606 bp open reading frame was cloned from V. fischeri that encoded a protein, which we named LitR,

55 we have cloned a gene, designated flrA, from V. fischeri that encodes a putative sigma(54)-dependent

56 clone a cross-hybridizing DNA fragment from V. fischeri genomic DNA.

57 in combination with L(H) or luciferase from V. fischeri (L(F)).

58 s induced three- to fourfold both as growing V. fischeri cells approach stationary phase and upon the

59 ation mediated by the syp gene cluster helps V. fischeri transition from a dispersed planktonic lifes

60 In V. fischeri, luxi directs the synthesis of N-(3-oxohexan

61 this model, we found that bioluminescence in V. fischeri ES114 is modulated by glucose and stimulated

62 A is the predominately expressed catalase in V. fischeri and indicating that V. fischeri carries only

63 ophore synthesis and symbiosis competence in V. fischeri that involves the glnD gene.

64 ify other regulators of biofilm formation in V. fischeri, we screened a transposon library for mutant

65 ibuted to RscS-mediated biofilm formation in V. fischeri.

66 ride loci contribute to biofilm formation in V. fischeri.

67 al strain, indicating that AI-2 functions in V. fischeri to delay luminescence induction.

68 AI-2 apparently functions in V. fischeri to suppress or delay induction at low and in

69 in modulating other symbiotic functions, in V. fischeri.

70 rge-scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the

71 ajor contributor to TMAO-dependent growth in V. fischeri under the conditions tested.

72 Genetic arrangement of the flrA locus in V. fischeri is similar to motility master-regulator oper

73 ited induction in a dose-dependent manner in V. fischeri and Escherichia coli carrying the lux genes.

74 Moreover, in V. fischeri, we observed ainR-dependent repression of a

75 Finally, a single mutation in V. fischeri-specifically, deletion of the lux operon, wh

76 onal regulation of TMAO reductase operons in V. fischeri appears to differ from that in previously st

77 regions of a number of flagellar operons in V. fischeri revealed apparent sigma(54) recognition moti

78 nnection between the Lux and Syp pathways in V. fischeri, and furthers our understanding of how the L

79 We report the presence in V. fischeri of ompU, a gene encoding a 32.5-kDa protein

80 ously identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on Lux

81 establishing that there is a LuxR regulon in V. fischeri MJ-100 whose genes are coordinately expresse

82 more fully characterize the LuxR regulon in V. fischeri MJ-100, real-time reverse transcription-PCR

83 cyl-HSL-responsive quorum-sensing regulon in V. fischeri.

84 molecule that facilitates quorum sensing in V. fischeri and is important for efficient symbiont asse

85 ry component of the quorum-sensing system in V. fischeri, promotes host colonization.

86 ggest that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expres

87 plication of molecular genetic techniques in V. fischeri.

88 ure for the introduction of plasmid DNA into V. fischeri by electroporation, and isolated a mutant st

89 istent with that activity, introduction into V. fischeri of medium-copy plasmids carrying these genes

90 ulates the aphrodisiac-like activity of live V. fischeri.

91 when colonizing the light organ of the model V. fischeri host, the Hawaiian bobtail squid Euprymna sc

92 We recently identified a novel V. fischeri locus, ainS, necessary for the synthesis of

93 lipopolysaccharide (LPS) or free lipid A of V. fischeri can trigger morphological changes in the juv

94 r, these results suggest the biogeography of V. fischeri populations within the squid light organ imp

95 When grown in culture, cells of V. fischeri strain PMF8, in which litR was insertionally

96 Coculture of V. fischeri with the Aeromonas sp. ARM81 lysogen suppres

97 e did not compromise symbiotic competence of V. fischeri; however, levels of colonization of an ainS

98 imal host and presents the first examples of V. fischeri genes that affect normal host tissue develop

99 er, these results show that the flagellum of V. fischeri is a complex structure consisting of multipl

100 nt, a galactose-utilization mutant (galK) of V. fischeri colonized juvenile squid to wild-type levels

101 imilation and to contribute to the growth of V. fischeri on cystine, which is the oxidized form of cy

102 rate here that the flavohaemoglobin, Hmp, of V. fischeri protects against NO, both in culture and dur

103 ing Euprymna scolopes, the symbiotic host of V. fischeri.

104 In this study, we investigated the impact of V. fischeri LuxS on luminescence and colonization compet

105 r this association, we searched a library of V. fischeri transposon insertion mutants for those that

106 We found the luminescence of V. fischeri to be correlated with external osmolarity bo

107 with a recently developed metabolic model of V. fischeri provides us with a clearer picture of the me

108 ring symbiosis onset and, (ii) modulation of V. fischeri growth in symbiotic maintenance.

109 cyclic di-GMP in the control of motility of V. fischeri.

110 Importantly, the acs mutant of V. fischeri has a competitive defect when colonizing the

111 A Deltahmp mutant of V. fischeri initiates squid colonization less effectivel

112 n to high density, we identified a mutant of V. fischeri that exhibited an apparent defect in symbios

113 ed and characterized a glnD:mTn5Cm mutant of V. fischeri.

114 Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-ace

115 Here, we asked whether OMVs are part of V. fischeri biofilms.

116 x genes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ai

117 light-organ crypts, where the population of V. fischeri cells resides.

118 amine transferase for the lipid-A portion of V. fischeri lipopolysaccharide.

119 n aggregation, suggest a two-step process of V. fischeri cell engagement: association with host cilia

120 o significantly decreased the growth rate of V. fischeri in culture.

121 ays, we identified three novel regulators of V. fischeri luminescence and seven regulators that alter

122 to host-like pH increased the resistance of V. fischeri to the cationic antimicrobial peptide polymi

123 light organ, in which case, the response of V. fischeri to NO is of considerable interest.

124 Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbi

125 n this system include the genome sequence of V. fischeri, an expressed sequence tagged library for E.

126 ent, we constructed a non-luminous strain of V. fischeri (delta luxA::erm).

127 iotic resistance marker to another strain of V. fischeri.

128 sest known relatives, flrA mutant strains of V. fischeri ES114 were completely abolished in swimming

129 ecular genetic techniques, mutant strains of V. fischeri have been constructed that are defective at

130 on sites occurs between different strains of V. fischeri, with the lancet-like type VI secretion syst

131 Through phenotypic studies of V. fischeri mutants we have found that the AinS-signal i

132 The study of V. fischeri HnoX permits us to understand not only host-

133 r NADase activity in culture supernatants of V. fischeri, and this mutant initiated the light organ s

134 To understand environmental influences on V. fischeri motility, we investigated migration of this

135 Like other gram-negative organisms, V. fischeri expresses lipopolysaccharide (LPS) on its ce

136 ing a chemoattractant gradient that promotes V. fischeri migration into host tissues.

137 Hmp protects V. fischeri from NO inhibition of aerobic respiration, a

138 Purified V. fischeri LPS, as well as the LPS of V. cholerae, Haem

139 e limitation or (ii) a mutation that renders V. fischeri defective in the synthesis of a homolog of t

140 finds quorum sensing unexpectedly represses V. fischeri's type 6 secretion system, highlighting intr

141 experiments demonstrate that the E. scolopes-V. fischeri system is a viable model for the experimenta

142 re prone to later superinfection by a second V. fischeri strain.

143 les that are produced by the squid to select V. fischeri from the ocean microbiota.

144 Here, we show that a single V. fischeri protein, the previously uncharacterized EroS

145 nside the light-emitting organ of the squid, V. fischeri experiences, recognizes and adjusts to the c

146 symbionts, is absent from the fish symbiont V. fischeri MJ11.

147 athogen Vibrio cholerae, the marine symbiont V. fischeri, and the opportunistic pathogen Pseudomonas

148 In the squid symbiont V. fischeri ES114, RscS controls light-organ colonizatio

149 ntified TMAO reductase activity in symbiotic V. fischeri isolates associated with the light organs of

150 le the luminescence (lux) genes of symbiotic V. fischeri have been shown to be highly induced within

151 ast 9 aa to the growing culture of symbiotic V. fischeri present in its light-emitting organ.

152 We found that His-tagged V. fischeri CRP could bind sequences upstream of both lu

153 is a component of the host defense, and that V. fischeri uses a cytotoxin-like molecule to induce hos

154 This demonstrates that V. fischeri quorum sensing regulates a substantial numbe

155 luminescence autoinducer, demonstrating that V. fischeri makes no luminescence autoinducers other tha

156 ork thus reveals a novel group of genes that V. fischeri controls through a sigma54-dependent respons

157 Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation an

158 catalase in V. fischeri and indicating that V. fischeri carries only a single catalase gene.

159 ecular Microbiology, Dunn et al. report that V. fischeri produces an NO-inducible and NO-resistant al

160 emical analysis of this mutant revealed that V. fischeri hvn null still possessed ADP-ribosyltransfer

161 Finally, we show that V. fischeri, purified EroS, and other bacterial chondroi

162 The results suggest that V. fischeri may help modulate the host stress responses

163 Our data suggest that V. fischeri normally senses a host-generated NO signal t

164 e could establish symbiosis, suggesting that V. fischeri acquires nutrients related to this compound

165 active during colonization, suggesting that V. fischeri symbionts are exposed to NO.

166 The V. fischeri luxI mutant does not express detectable ligh

167 The V. fischeri mutant grew poorly with galactose as a sole

168 On addition of the acceptor, the V. fischeri yellow fluorescence protein containing eithe

169 own to be differentially expressed among the V. fischeri populations occupying the various colonizati

170 essful colonization of E. scolopes, i.e. the V. fischeri ainS mutant failed to persist in the squid l

171 l early stage of symbiotic initiation in the V. fischeri-squid model symbiosis, and more broadly it a

172 A null mutation introduced into the V. fischeri ompU resulted in the loss of an OMP with an

173 e but may also use this light to monitor the V. fischeri population.

174 We have investigated the impact of the V. fischeri acyl-HSL synthase AinS on both luminescence

175 trate in the luxI-dependent synthesis of the V. fischeri autoinducer.

176 Comparison of the V. fischeri ES114 genome with that of conspecific strain

177 y between E. coli Pgm and the product of the V. fischeri gene, which was therefore designated pgm.

178 Analysis of the V. fischeri genome revealed the presence of a putative t

179 e, it is possible that these features of the V. fischeri lipid A may underlie the ability of E. scolo

180 The expression of the V. fischeri OmpU was not significantly affected by eithe

181 tical issues concerning the synthesis of the V. fischeri signal.

182 In contrast, the luminescence of the V. fischeri symbionts is optimal above 24 degrees C and

183 s to the already considerable utility of the V. fischeri-E. scolopes model system.

184 e wild-type and waaL strains showed that the V. fischeri LPS has a single O-antigen repeat composed o

185 Our results show that the V. fischeri waaL mutant has a motility defect, is signif

186 moserine lactone structurally related to the V. fischeri and other autoinducers.

187 on the ainS promoter depended on whether the V. fischeri regulatory gene litR was also introduced.

188 nescent strains exhibit monophyly within the V. fischeri clade.

189 onent response regulators encoded within the V. fischeri genome.

190 Hmp, influence aggregate size and, thereby, V. fischeri colonization efficiency.

191 ts reduced the inhibition of luminescence to V. fischeri, i.e., were beneficial for the bacteria, wit

192 compared and analyzed the ain locus from two V. fischeri strains and a Vibrio salmonicida strain to e

193 a glycerol/tryptone-based medium, wild-type V. fischeri cells initially excrete acetate but, in a me

194 was localized in the periplasm of wild-type V. fischeri cells, where its role could be to detoxify h

195 predicted, in the presence of NO, wild-type V. fischeri grew more slowly on hemin than a hnoX deleti

196 evels relative to that achieved by wild-type V. fischeri.

197 These studies indicate that the unusual V. fischeri O-antigen sugars play a role in the early ph

198 This study lays the foundation for using V. fischeri as a model system for studying TMAO reductas

199 When V. fischeri cells were introduced into the seawater surr

200 This light organ symbiosis is initiated when V. fischeri cells present in the surrounding seawater en

201 ity by SypE is a critical mechanism by which V. fischeri controls biofilm development and symbiotic c

202 Thus, this work reveals a mechanism by which V. fischeri inhibits cellulose-dependent biofilm formati

203 o play a role in the normal process by which V. fischeri initiates its colonization of the nascent li

204 surrounding mucus, the environment in which V. fischeri cells aggregate before migration into the or

205 ent work suggests that the tissue with which V. fischeri associates not only can detect bioluminescen

206 ce, whereas the protein was colocalized with V. fischeri in the LO-CC.

207 similar to that triggered by infection with V. fischeri.

208 First, animals inoculated with V. fischeri aboard the space shuttle showed effective co

209 ymna scolopes forms a natural symbiosis with V. fischeri, and utilizes the symbiont-derived biolumine