ライフサイエンスコーパス: X. laevis

1 X. laevis GPRx is mainly expressed in brain, ovary, and

2 X. laevis IFN and IFN-lambda displayed distinct tissue e

3 X. laevis microtubules combine very fast growth and infr

4 X. laevis oocytes expressing mutant Mtnip-1 and Mtlatd w

5 X. laevis S(mu)-mediated CSR occurred mostly in a region

6 Using a X. laevis kidney-derived cell line, we show that enhanci

7 omparisons, it is likely that the additional X. laevis genes arose from tetraploidization.

8 itical for early antiviral immunity in adult X. laevis.

9 Hence, as predicted, virus-infected adult X. laevis frogs exhibited significantly more robust FV3-

10 Polyclonal antibodies raised against X. laevis pcMTase were immunoreactive with a 33-kDa prot

11 , and functional restoration of an amputated X. laevis hindlimb following a 24-hour exposure to a mul

12 Accordingly, an X. laevis type I interferon was identified, the expressi

13 ordingly, we identified and characterized an X. laevis type I interferon in the context of infection

14 lectin with the bovine spleen galectin-1 and X. laevis skin galectin, it should be concluded that wit

15 pported prereplication complex formation and X. laevis sperm DNA replication, whereas the complex wit

16 e enriched on microtubules in both human and X. laevis.

17 e morphological differences, X. longipes and X. laevis embryogenesis occurred with similar timing, wi

18 alized maternal mRNAs in D. melanogaster and X. laevis.

19 segment axoneme when expressed in mouse and X. laevis photoreceptors, whereas KIF3A was restricted t

20 s genome template amounts, embryo sizes, and X. laevis maternal environments.

21 Comparison of X. tropicalis and X. laevis blots revealed comparable expression profiles,

22 identified miRNAs in both X. tropicalis and X. laevis.

23 and have evolved to about the same extent as X. laevis and Xenopus borealis TFIIIAs.

24 riptome was differentially expressed between X. laevis and X. muelleri and there were more genes that

25 road conservation of gene expression between X. laevis and other gnathostomes, we also identified sev

26 b3) was positively regulated by Ets1 in both X. laevis and X. tropicalis.

27 tance of Cdk for this interaction using both X. laevis egg extracts and human cells.

28 Minigenes containing X. laevis spacer sequences are only dominant over minige

29 adenylation, suggesting that the cytoplasmic X. laevis form of the 30-kDa subunit of CPSF is involved

30 ertebrate development, in that WDR5-depleted X. laevis tadpoles exhibit a variety of developmental de

31 f ectopic tail-like structures in developing X. laevis embryos.

32 ic expression patterns of these genes during X. laevis development.

33 epithelial rearrangements that occur during X. laevis development.

34 gnalling molecules in the Wnt pathway during X. laevis gastrulation.

35 ey role of gp69/64 as sperm receptors during X. laevis fertilization.

36 he analysis of their expression during early X. laevis development and in adult tissues.

37 ributes to nuclear size changes during early X. laevis development.

38 We have examined lbx1 expression in early X. laevis tadpoles.

39 ves and anterior-most spinal nerves of early X. laevis tadpoles.

40 g of how Notch signalling patterns the early X. laevis pronephros anlagen, a function that might be c

41 of either XenU1 or XenU1a with NR1 in either X. laevis oocytes and human embryonic kidney (HEK) 293 c

42 bining X. tropicalis egg extract with either X. laevis or X. borealis sperm chromosomes revealed that

43 rog X. tropicalis are fertilized with either X. laevis or X. borealis sperm.

44 us laevis that overexpress the cDNA encoding X. laevis GH.

45 is located upstream of the 42-bp enhancers; X. laevis enhancers alone are not sufficient.

46 Thus, the entire X. laevis rDNA intergenic spacer (the 0 repeats, 1 repea

47 o characterize Flt3 cell surface expression, X. laevis-tagged rFlt3lg.S and rFlt3lg.L were produced.

48 94.2 mug Se/g dry mass (d.m.), adult female X. laevis were bred with untreated males, and resulting

49 al removal of the ovaries from mature female X. laevis, the dissection of individual oocytes, then th

50 N) by using X. tropicalis sperm to fertilize X. laevis eggs with or without their maternal genome.

51 d genomic sequence information available for X. laevis, we used RNA-seq to comprehensively identify t

52 by a viral promoter, rat promoter, and four X. laevis promoters were all unaffected by passage throu

53 izer (SMO) in the South African Clawed Frog (X. laevis), a popular model of development, has long bee

54 ing phosphorylation site that is absent from X. laevis.

55 n galectins-1, but not in the galectins from X. laevis, fish, and invertebrates described so far.

56 Mitochondrial lysates from X. laevis oocytes contain both DNA ligase III alpha and

57 arate and identify the oligosaccharides from X. laevis egg jelly layers.

58 Mucus secretions from X. laevis previously exposed to B. dendrobatidis contain

59 Furthermore, X. laevis' sex-determining gene, DM-W, does not exist in

60 to the maintenance of G2-arrest in immature X. laevis oocytes.

61 In X. laevis oocytes expressing ten individual urate transp

62 In X. laevis oocytes expressing the cloned mouse PCFT, fola

63 In X. laevis oocytes, which are translationally saturated a

64 In X. laevis, progesterone activates the G2-arrested oocyte

65 neurons and inhibited anesthetic actions in X. laevis tadpoles.

66 lectivity for Suc and high Suc affinities in X. laevis oocytes at pH 5-SbSUT1, 6.3 +/- 0.7 mm, and Sb

67 n mammalian FGF8 spliceforms are analyzed in X. laevis, the contrast in activity is conserved.

68 cheal epithelial cell (mTEC) cultures and in X. laevis embryos treated with Gas2l2 morpholinos.

69 cell-free system for centromere assembly in X. laevis egg extracts, we discover two activities that

70 gnizes a single approximately 28 kDa band in X. laevis CNS and rat cerebellum.

71 hat superficial presumptive somitic cells in X. laevis ingress into the deep region as bottle cells w

72 n to enabling proteomics on smaller cells in X. laevis, we also demonstrated this technology to be sc

73 ine undergoes similar metamorphic changes in X. laevis and X. tropicalis, making it possible to use t

74 ing of recombinant Ca(v)2.1 Ca2+ channels in X. laevis oocytes; 2) gabapentin inhibition occurs in th

75 21S.p., form a complex similar to cohesin in X. laevis.

76 niofacial tissues are naturally corrected in X. laevis tadpoles has provided valuable insights into t

77 condition, CaT2 promoted inward currents in X. laevis oocytes upon external application of Ca(2+).

78 Notably, increasing blood vessel density in X. laevis limbs, as well as incubating tadpoles under hi

79 llar structure of basal ROS and COS disks in X. laevis photoreceptors.

80 Neural ectoderm in X. laevis consists of two components, a superficial laye

81 s can produce high frequency gene editing in X. laevis, permitting analysis in the F0 generation, and

82 Agonist-stimulated calcium efflux in X. laevis oocytes or inositol phosphate accumulation in

83 to efficiently create transgenic embryos in X. laevis and may increase the practical use of phiC31 i

84 es on each slide representing the entries in X. laevis UniGene Build 48.

85 in differentiation of a simple epithelium in X. laevis and D. rerio.

86 at neuronal nicotinic receptors expressed in X. laevis oocytes display appropriate pharmacological pr

87 cation (HCN) pacemaker channels expressed in X. laevis oocytes using site-directed mutagenesis and th

88 esidues were nonfunctional when expressed in X. laevis oocytes, but their ability to form tetramers i

89 an at any of the GluCl subunits expressed in X. laevis oocytes.

90 uired for channel function when expressed in X. laevis oocytes.

91 cation channels with alpha6 by expression in X. laevis oocytes alone or in pairwise combination with

92 ted down-regulation of treacle expression in X. laevis oocytes resulted in inhibition of rDNA gene tr

93 estigation of neural convergent extension in X. laevis and further our understanding of convergent ex

94 ty underlying neural convergent extension in X. laevis are the first high-resolution video documentat

95 ial transcription of X. laevis rRNA genes in X. laevis x Xenopus borealis hybrids, an epigenetic phen

96 Confocal analysis of immunofluorescence in X. laevis oocytes expressing the wild type and the three

97 n unsuspected role for biliverdin IXalpha in X. laevis embryogenesis.

98 icalis extracts, and elevated TPX2 levels in X. laevis extracts reduced spindle length and sensitivit

99 e intestine and the typhlosole, just like in X. laevis.

100 the ease of establishing transgenic lines in X. laevis.

101 ene expression map of the head mesenchyme in X. laevis during early larval development, focusing on t

102 s and diaphragmatico-branchialis muscles) in X. laevis.

103 assembly in tissue culture cells but not in X. laevis egg extracts.

104 Checkpoint activation occurs in X. laevis egg extracts upon addition of an oligonucleoti

105 show that CoA inhibits apoptosis not only in X. laevis oocytes but also in Murine oocytes.

106 GABA-activated current in VTA neurons or in X. laevis oocytes expressing alpha2beta3gamma2 GABA(A) r

107 aneously altering nuclear size and ploidy in X. laevis embryos.

108 Consistently, perturbation of Ppp4c in X. laevis embryos interfered with normal embryogenesis a

109 3 is co-expressed with 5-HT(3A) receptors in X. laevis oocytes.

110 t evidence that FGF8 performs a dual role in X. laevis and X. tropicalis early development.

111 vity, nor do they play a discernible role in X. laevis-X. borealis rRNA gene competition.

112 ed Cdk consensus target sequence (on S976 in X. laevis and S1000 in humans).

113 e points from the corresponding sequences in X. laevis, each by a single substitution.

114 rst quantify blastomere and nuclear sizes in X. laevis embryos, demonstrating that the N/C volume rat

115 Heterologous expression of human SLC5A8 in X. laevis oocytes induced Na(+)-dependent inward current

116 emination into the central nervous system in X. laevis tadpole but not adult.

117 The results of this study indicate that, in X. laevis, the true biological function of multivalency

118 ion to an approximately 1.4-kb transcript in X. laevis fat body and oocytes, whereas a weaker signal

119 at depletion of maternal irf6 transcripts in X. laevis embryos leads to gastrulation defects and rupt

120 of the ability to carry out transgenesis in X. laevis and gene knockdown in X. tropicalis, we demons

121 ein were tested for their ability to inhibit X. laevis fertilization.

122 -K mediated outward current in hSlo injected X. laevis oocytes.

123 laevis embryonic neuronal culture and intact X. laevis embryos, that the nerve growth-promoting actio

124 erent GVS electrode placement in semi-intact X. laevis preparations and humans and the more global ac

125 ake of dehydroascorbic acid and glucose into X. laevis oocytes.

126 udies, HLE-B3 poly(A)+ RNA was injected into X. laevis oocytes and GSH uptake experiments were perfor

127 pecific polyclonal antibodies to xNup98 into X. laevis oocytes.

128 sing pan-cancer analysis and Xenopus laevis (X. laevis) embryo model.

129 In large X. laevis oocytes, gravity becomes a dominant force and

130 ensing nose (principal cavity; PC) of larval X. laevis is respecified into an air-sensing cavity in a

131 Male X. laevis suffered a 10-fold decrease in testosterone le

132 the apoptotic inhibition observed in meiotic X. laevis egg extracts.

133 n of craniofacial defects in pre-metamorphic X. laevis tadpoles.

134 We have cloned a novel X. laevis GPCR, GPRx, which may play a similar role to G

135 Moreover, biochemical analysis of X. laevis embryos demonstrated that both endogenous and

136 l facilitate the development and analysis of X. laevis models of inherited retinal degeneration.

137 r CPSF is also localized to the cytoplasm of X. laevis oocytes.

138 predominantly localized to the cytoplasm of X. laevis oocytes.

139 adiol (EE2) during sexual differentiation of X. laevis were evaluated by use of Illumina sequencing c

140 ns of these proteins during embryogenesis of X. laevis.

141 In embryos of X. laevis, and many other species, early development req

142 Our findings indicate that enrichment of X. laevis skin mast cells confers anti-Bd protection and

143 The results show that erosion of X. laevis genes and functional regulatory elements is as

144 oth CPSF and CPEB are present in extracts of X. laevis oocytes prepared before or after meiotic matur

145 esent 133 new, high-quality illustrations of X. laevis development from fertilization to metamorphosi

146 However, our knowledge of X. laevis protein post-translational modifications remai

147 ty of liposomes made from membrane lipids of X. laevis eggs.

148 RESULTS We reconstructed the CR map of X. laevis NPC at 6.9 and 6.7 angstrom resolutions for th

149 phaFold because no high-resolution models of X. laevis Nups were available.

150 d in the mitotic phosphoprotein 43 (MP43) of X. laevis.

151 is is blocked, just as it does in oocytes of X. laevis.

152 We characterize the allotetraploid origin of X. laevis by partitioning its genome into two homoeologo

153 r segments of rod and cone photoreceptors of X. laevis.

154 X. tropicalis is a close relative of X. laevis that shares the same ease of tissue manipulati

155 hich is a small, faster-breeding relative of X. laevis, has recently been adopted for research in dev

156 e defenses are involved in the resistance of X. laevis to lethal B. dendrobatidis infections.

157 We investigated the respective roles of X. laevis type I and type III interferons (IFN and IFN-l

158 as not detectable during the early stages of X. laevis development, and remained low in the adult tis

159 s laevis Comparison of modeled structures of X. laevis Flt3 and Flt3lg homoeologs with the related di

160 much of what we have learned from studies of X. laevis oocytes holds for those of X. tropicalis, and

161 ems supplement transgenesis for the study of X. laevis metamorphosis, one system controlled by the pr

162 Second, the 100-kDa subunit of X. laevis CPSF forms a specific complex with RNAs that c

163 d, immunodepletion of the 100-kDa subunit of X. laevis CPSF reduces CPE-specific polyadenylation in v

164 X. borealis sequence with respect to that of X. laevis.

165 r several practical advantages over those of X. laevis, including faster and more synchronous meiotic

166 pacers differ very extensively from those of X. laevis.

167 elative to terrestrial anurans, the torus of X. laevis is hypertrophied and occupies the entire cauda

168 omoters to the preferential transcription of X. laevis (over X. borealis) rRNA genes has never been t

169 to explain the preferential transcription of X. laevis rRNA genes in X. laevis x Xenopus borealis hyb

170 F0 generation, and advancing the utility of X. laevis as a subject for biological research and disea

171 yngeal motor neurons in nucleus (N.) IX-X of X. laevis are calbindin-positive.

172 the large amount of information available on X. laevis intestinal metamorphosis and the genome sequen

173 Active mitotic translation occurs on X. laevis meiotic spindles, and a subset of microtubule-

174 ether channels are studied in yeast cells or X. laevis oocytes.

175 SbSUT4 could not be detected using yeast or X. laevis oocytes.

176 g early embryogenesis in the model organisms X. laevis and C. elegans, the spindle scales with cell s

177 nisms, especially at genome-wide level, over X. laevis, largely due to its shorter life cycle and seq

178 he cardiac regenerative outcome, and present X. laevis as an alternative model to decipher the develo

179 In addition, NH-3 prevents X. laevis thyroid hormone receptors from binding to the

180 Previously, X. laevis has been shown to express both NF-L and XNIF,

181 Pretreatment with a recombinant X. laevis IFN (rXlIFN) substantially reduced viral repli

182 However, a recombinant X. laevis IFN-lambda (rXlIFN-lambda) conferred less prot

183 binant forms of these cytokines (recombinant X. laevis IFN [rXlIFN] and rXlIFN-lambda) elicited antiv

184 combinant form of this molecule (recombinant X. laevis interferon [rXlIFN]) was produced for the purp

185 The recombinant X. laevis receptor has a distinct pharmacological profil

186 e also observed for two other highly related X. laevis genes, xmdc13 and adam13.

187 In combination with the previously reported X. laevis opsin and arrestin promoters, these sequences

188 (14)(,)(15) The most widely studied species, X. laevis (4N = 36) and X. tropicalis (2N = 20), scale a

189 und in vivo, we used induced and spontaneous X. laevis tadpole metamorphosis, a thyroid hormone-depen

190 The extracellular matrix surrounding X. laevis eggs consists of a vitelline envelope and a je

191 ti-Fv3 protection to the virally susceptible X. laevis kidney (A6) cell line.

192 spindles were approximately 30% shorter than X. laevis spindles, and mixing experiments revealed a dy

193 Presently, we demonstrate that X. laevis tadpoles are intrinsically more resistant to F

194 In contrast to our previous findings that X. laevis tadpoles exhibit delayed and modest type I IFN

195 Together, these findings imply that X. laevis XNC10-restricted iValpha6 T cells play importa

196 The X. laevis genome consists of two subgenomes, referred to

197 The X. laevis vocal CPG produces a 50-60 Hz "fast trill" son

198 However, although the X. laevis chromosomes maintained centromeric CENP-A in m

199 variable" (type II) CRDs, represented by the X. laevis 16-kDa galectin, clearly constitute distinct s

200 tin secreted into environmental water by the X. laevis embryo, is postulated to function as a defense

201 e receptors, we isolated a cDNA encoding the X. laevis brain CCK receptor (CCK-XLR).

202 These data indicate a role for the X. laevis MRN complex in MMEJ.

203 of fluorescent dextran amines identifies the X. laevis central amygdala (CeA) as a target for ascendi

204 s three lines of evidence that implicate the X. laevis homologue of the 100-kDa subunit of CPSF in th

205 and flexibility of expression cloning in the X. laevis embryo.

206 -secreting cells (PSCs) differentiate in the X. laevis larval skin soon after gastrulation, based on

207 rs of the lysophosphatidate receptors in the X. laevis oocyte.

208 To assess innovations in the X. laevis subgenomes we examined p300-bound enhancer pea

209 Our structures of the X. laevis Eco2 (xEco2) bound to its primary and secondar

210 Here we describe manipulation of the X. laevis genome using CRISPR/Cas9 to model the human di

211 odification, we undertook an analysis of the X. laevis phosphoproteome at seven developmental stages

212 ent in the hormone-dependent promoter of the X. laevis TRbetaA gene.

213 At room temperature, the X. laevis violet opsin has an absorption maximum at 426

214 ) cytokine and our findings suggest that the X. laevis IL4 plays a key role in manifesting the effect

215 We conclude that the X. laevis oocyte heterologous expression system is a val

216 Unlike the X. laevis galectin, the binding activity of the B. arena

217 d photoreceptors of Xenopus laevis using the X. laevis principal opsin promoter.

218 localize to nucleoli, and interact with the X. laevis Box C/D RNA binding proteins fibrillarin, Nop5

219 esults indicated that in comparison with the X. laevis CSF-1-Mphis, the IL-34-Mphis express substanti

220 ding domain (DBD) and the intact ER with the X. laevis vitellogenin A2 ERE and the human pS2 ERE.

221 We subsequently map this X. laevis LB3 phosphorylation site to a conserved site i

222 t positions that confer low TCDD affinity to X. laevis AHRs (A364, A380, and N335), and homology mode

223 he X. tropicalis spindle smaller compared to X. laevis [2], as do elevated levels of TPX2, a protein

224 slower in X. tropicalis extracts compared to X. laevis, but that this difference is unlikely to accou

225 in egg extracts of X. tropicalis compared to X. laevis.

226 e in premetamorphic tadpoles, in contrast to X. laevis, where it is present only in the anterior 1/3.

227 other hand, X. tropicalis, highly related to X. laevis, offers a number of advantages for studying de

228 ays, the EC50 for TCDD was 23 nM, similar to X. laevis AHR1beta (27 nM) and greater than AHR containi

229 Transgenic X. laevis tadpoles overexpressing a GFP-D3 fusion protei

230 constitute the first report of a transgenic X. laevis model of retinal degenerative disease.

231 for limb regeneration, we created transgenic X. laevis tadpoles that express Dickkopf-1 (Dkk1), a spe

232 ein (GFP) can be used to generate transgenic X. laevis embryos.

233 The authors generated transgenic X. laevis expressing the Escherichia coli enzyme nitrore

234 of ovine or Xenopus laevis PRL in transgenic X. laevis does not prolong tadpole life, establishing th

235 region to direct OS targeting in transgenic X. laevis retinas.

236 oters were tested for function in transgenic X. laevis.

237 Similarly, transgenic X. laevis expressing Ter349Glu rhodopsin exhibited parti

238 as increased in X. tropicalis, which, unlike X. laevis, lacks an inhibitory phosphorylation site in t

239 Using X. laevis egg extracts, we show that SSX2IP accumulated

240 ealed strong evolutionary conservation, with X. laevis and X. tropicalis possessing distinct and uniq

241 nd the enhancer landscape in X. tropicalis x X. laevis hybrid embryos.