ライフサイエンスコーパス: X. tropicalis

1 X. tropicalis is a close relative of X. laevis that shar

2 X. tropicalis Tmem16a functions as a voltage-gated, calc

3 Here we describe the analysis of 219,270 X. tropicalis expressed sequence tags (ESTs) from four e

4 ely studied species, X. laevis (4N = 36) and X. tropicalis (2N = 20), scale at all levels, from body

5 In both cultured cells and X. tropicalis embryos, membrane-bound Ephrins (Efns) B1

6 t FGF8 performs a dual role in X. laevis and X. tropicalis early development.

7 volutionary conservation, with X. laevis and X. tropicalis possessing distinct and unique alterations

8 similar metamorphic changes in X. laevis and X. tropicalis, making it possible to use the large amoun

9 vely regulated by Ets1 in both X. laevis and X. tropicalis.

10 cies, X. amieti, X. cliivi, X. petersii, and X. tropicalis, by developing ex vivo brain preparation f

11 ly affected in acbd6-deficient zebrafish and X. tropicalis models, including Fus, Marcks and Chchd-re

12 cytoplasm ("MPF activity") into G2-arrested X. tropicalis oocytes induces entry into meiosis I even

13 expression of many identified miRNAs in both X. tropicalis and X. laevis.

14 In both X. tropicalis extracts and the spindle simulation, a bal

15 In vitro reactions combining X. tropicalis egg extract with either X. laevis or X. bo

16 In contrast, X. tropicalis microtubules grow slower and catastrophe m

17 enome and compared it to the related diploid X. tropicalis genome.

18 However, comparison with diploid X. tropicalis and zebrafish shows broad conservation of

19 nce that tsg acts as a BMP antagonist during X. tropicalis gastrulation since the tsg depletion pheno

20 To establish X. tropicalis intestinal metamorphosis as a model for ad

21 frogs, using a dense meiotic linkage map for X. tropicalis and chromatin conformation capture (Hi-C)

22 mapping studies in the related diploid frog X. tropicalis, and for other reasons.

23 c lethality occurs when the eggs of the frog X. tropicalis are fertilized with either X. laevis or X.

24 tudies in the rapidly breeding diploid frog, X. tropicalis.

25 are unique to one subgenome and absent from X. tropicalis.

26 Scaffolds from X. tropicalis genome assembly 2.0 (JGI) were scanned for

27 On the other hand, X. tropicalis, highly related to X. laevis, offers a num

28 Hence, X. tropicalis is a useful model for the study of molecul

29 Resulting embryos have otherwise identical X. tropicalis genome template amounts, embryo sizes, and

30 Here we identify X. tropicalis' sex chromosome system by integrating data

31 In X. tropicalis, k-fiber MT bundles that connect to chromo

32 Indeed, TPX2 was threefold more abundant in X. tropicalis extracts, and elevated TPX2 levels in X. l

33 resource for genetic and genomic analyses in X. tropicalis.

34 the morphological and cytological changes in X. tropicalis intestine during metamorphosis.

35 everal additional ones that are conserved in X. tropicalis.

36 ex-determining gene, DM-W, does not exist in X. tropicalis, and the sex chromosomes in the two specie

37 in order for egg cytoplasm to induce GVBD in X. tropicalis oocytes.

38 tanin-dependent MT severing was increased in X. tropicalis, which, unlike X. laevis, lacks an inhibit

39 nsgenesis in X. laevis and gene knockdown in X. tropicalis, we demonstrate that endogenous Dot1L is c

40 the epigenome and the enhancer landscape in X. tropicalis x X. laevis hybrid embryos.

41 he first positional cloning of a mutation in X. tropicalis, we show that non-contractile hearts in mu

42 al for embryogenesis and premetamorphosis in X. tropicalis On the other hand, knocking out EVI and MD

43 icalis (Xt) allurin, a homologous protein in X. tropicalis.

44 Human disease features are replicated in X. tropicalis larvae with morpholino knockdowns, in whic

45 aled that microtubules polymerized slower in X. tropicalis extracts compared to X. laevis, but that t

46 derstanding the sex-determination systems in X. tropicalis is critical for developing this flexible a

47 LOGY/PRINCIPAL FINDINGS: We observed that in X. tropicalis, the premetamorphic intestine was made of

48 deep region as bottle cells whereas those in X. tropicalis ingress by "relamination".

49 Interestingly, X. tropicalis spindles were approximately 30% shorter th

50 ations in TRIO GEFD1 in the vertebrate model X. tropicalis induce defects that are concordant with th

51 ncing (RNA-Seq) on wild-type and ets1 mutant X. tropicalis embryos.

52 quence information and genetic advantages of X. tropicalis to dissect the pathways governing adult in

53 present a draft genome sequence assembly of X. tropicalis.

54 Comparison of X. tropicalis and X. laevis blots revealed comparable ex

55 shorter spindles observed in egg extracts of X. tropicalis compared to X. laevis.

56 Like that of other tetrapods, the genome of X. tropicalis contains gene deserts enriched for conserv

57 dies of X. laevis oocytes holds for those of X. tropicalis, and suggest that X. tropicalis oocytes of

58 and cell biological experiments, the use of X. tropicalis provides novel insight into the complex me

59 f pathobiology and underscore the utility of X. tropicalis as a model system for understanding neurod

60 We show that X. tropicalis egg extracts reconstitute the fundamental

61 for those of X. tropicalis, and suggest that X. tropicalis oocytes offer a good experimental system f

62 for a newly recognized "Crisp A" gene in the X. tropicalis genome.

63 es representation of a minimum of 66% of the X. tropicalis genome, incorporating 758 of the approxima

64 rotubule severing protein katanin scales the X. tropicalis spindle smaller compared to X. laevis [2],

65 Comparisons of this map with the X. tropicalis genome Assembly 4.1 (JGI) indicate that th

66 ategy, we identified unique SSLPs within the X. tropicalis genome.

67 eri form meiotic spindles similar in size to X. tropicalis but that TPX2 and katanin-mediated scaling

68 We generated a transgenic X. tropicalis line that expresses enhanced green fluores

69 s across the MBT in both conditions and used X. tropicalis paternally derived mRNA to identify a high

70 ifferences in genomic DNA (i.e., N) by using X. tropicalis sperm to fertilize X. laevis eggs with or

71 of brain and craniofacial structures within X. tropicalis embryos upon dyrk1a and six1 loss of funct

72 Strikingly, young X. tropicalis DNA transposons are derepressed and recrui