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1 sidue near the carboxyl terminus (Ser-883 in Xenopus).
2 entity in the secondarily aquatic frog genus Xenopus.
3 s pivotal for CNC induction and migration in Xenopus.
4 ur long-standing research program focused on Xenopus, a frog genus which has provided valuable insigh
5 ails in the tuatara and regenerated limbs in Xenopus adult frogs, which have a cartilaginous endoskel
7 evolutionary relationship with humans, makes Xenopus an attractive model to uncover the mechanisms un
10 DAPLE, MPDZ is induced during neurulation in Xenopus and is required for apical constriction of neuro
11 DNA demethylation in non-mammalian species (Xenopus and medaka) despite their evolutionary divergenc
13 expression patterns in the thalamus between Xenopus and mouse, however, the dynamic changes in gene
14 results in decreased levels of rfx3 mRNA in Xenopus, and exogenous rfx3 can rescue the Rnf20 depleti
15 The similarities of CSF-c cells in chicken, Xenopus, and zebrafish suggest that these characteristic
16 XR1 depletion is perinatally lethal in mice, Xenopus, and zebrafish; however, the mechanisms driving
19 positively regulates vegfa expression during Xenopus blood stem cell development through multiple tra
20 a stiffness gradient arose in the developing Xenopus brain, and retinal ganglion cell axons turned to
23 s species, their ratio is shared within each Xenopus clade providing information on species identity
26 ma-subunit combinations, we demonstrate that Xenopus deltabetagamma-ENaC is profoundly activated by e
27 ue-specific splicing of fxr1 is required for Xenopus development and alters the disordered domain of
28 we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous compo
31 Overexpressed Rspo2 inhibited elongation of Xenopus ectoderm explants and Erk1 activation in respons
33 the Tetrahymena group I ribozyme embedded in Xenopus egg extract demonstrate the ability of M2-seq to
34 ysical model by titrating dynein activity in Xenopus egg extract spindles and quantifying the shape a
38 the polarity-independent sliding observed in Xenopus egg extracts and in vitro experiments with purif
39 movement by dynein and actomyosin forces in Xenopus egg extracts and observed outward co-movement of
41 orks, generated by encapsulating cytoplasmic Xenopus egg extracts into cell-sized 'water-in-oil' drop
45 te the signal that triggers CMG unloading in Xenopus egg extracts using single-molecule and ensemble
59 we have studied neuronal development in the Xenopus embryo in the absence of n1-src, while preservin
62 We have identified a case of regeneration in Xenopus embryonic aggregates that restores a mucociliate
65 of a set of innate immune response genes in Xenopus embryos and that splicing-blocking morpholinos l
68 esis in human fetuses, and sox4 knockdown in Xenopus embryos diminishes brain and whole-body size.
69 ansiently inhibits neural crest migration in Xenopus embryos in a Snail1-dependent manner, indicating
72 phenotypes were further confirmed in MCCs of Xenopus embryos when CAMSAP3 expression was knocked down
75 re experiments, promoted axis duplication in Xenopus embryos, stimulated low-density lipoprotein rece
82 chnique for cells (Xenopus oocyte), tissues (Xenopus epithelium and rat cornea), organs (Xenopus gill
84 wn to unhook interstrand crosslinks (ICL) in Xenopus extracts, how NEIL3 participants in ICL repair i
86 s in tubulin populations between two related Xenopus frog species influence microtubule dynamics and
92 trives to integrate the body of knowledge on Xenopus genomics and biology together with the visualiza
93 (Xenopus epithelium and rat cornea), organs (Xenopus gills and mouse skin) and appendages (Xenopus ta
95 ration research and highlight how studies of Xenopus have led to new insights into the mechanisms gov
96 T imaging planes to visualize and quantitate Xenopus heart and facial structures establishing normati
97 corrected by the addition of exogenous Xkid (Xenopus homolog of human Kid/KIF22), indicating a role f
98 y well understood, but neither zebrafish nor Xenopus is electroreceptive and our molecular understand
102 dy, we have analyzed in the anuran amphibian Xenopus laevis (an anamniote vertebrate), through larval
104 ate post-anaphase microtubule (MT) asters in Xenopus laevis and other large eggs remains unclear.
105 ciated proteins in the egg cytoplasm between Xenopus laevis and Xenopus tropicalis have been shown to
107 ins purified from two closely related frogs, Xenopus laevis and Xenopus tropicalis, have surprisingly
110 g nanobody-labeled nuclear pore complexes in Xenopus laevis cells showed that MINFIELD-STED microscop
111 transient protein interactions in cell-free Xenopus laevis egg extract identified the dimeric histon
112 that combines microfluidics, hydrogels, and Xenopus laevis egg extract to investigate the mechanics
116 Here, we demonstrate biochemically using Xenopus laevis egg extracts that the Cdk1-counteracting
120 ere, we used cell-free extracts derived from Xenopus laevis eggs to recapitulate different phases of
122 for improved tracking of calcium flux in the Xenopus laevis embryo, lowering the barrier for in vivo
125 ctions in both cell and nuclear sizes during Xenopus laevis embryogenesis provide a robust scaling sy
126 ing analysis of >1600 proteins from ~130 mum Xenopus laevis embryonic cells containing <6 nL of cytop
132 dermal cells and tissues from gastrula stage Xenopus laevis embryos demonstrate that deletion of extr
134 xpressed specifically in the neural plate of Xenopus laevis embryos to trigger a G protein signaling
135 pic Barrier Assay (ZnUMBA), which we used in Xenopus laevis embryos to visualize short-lived, local b
137 and testing the effects of phosphomutants in Xenopus laevis embryos, we identify the novel site S267
147 In this manuscript, we took advantage of Xenopus laevis models of both sexes expressing wild-type
148 the other DNA glycosylases NEIL1 and NEIL2, Xenopus laevis NEIL3 C terminus has two highly conserved
152 PIP2;1 in the plant and upon coexpression in Xenopus laevis oocytes and activated AtPIP2;1, preferent
153 od pressure values"Functional experiments in Xenopus laevis oocytes and HEK293T cells demonstrated th
154 localize to the tonoplast; when expressed in Xenopus laevis oocytes and Nicotiana benthamiana cells,
155 electrode voltage clamp electrophysiology in Xenopus laevis oocytes and radioligand displacement assa
157 spinal cord neurons, spinal cord slice, and Xenopus laevis oocytes expressing recombinant human glyc
159 cted in the parasite and in PfCRT-expressing Xenopus laevis oocytes linked phosphomimetic substitutio
161 heteromeric 5-HT(3AB) receptors expressed in Xenopus laevis oocytes using two-electrode voltage clamp
162 died their effects on GABA(A)Rs expressed in Xenopus laevis oocytes using two-microelectrode voltage
163 es including placental villous fragments and Xenopus laevis oocytes were used to investigate UDCA tra
164 perties heterologously expressed in yeast or Xenopus laevis oocytes, and their in planta cellular and
165 ecific glycosylation sites were expressed in Xenopus laevis oocytes, HEK-293T cells, and HeLa cells.
166 able to interact with the cell membranes of Xenopus laevis oocytes, to alter their electrical membra
167 by endogenous TMEM16A channels expressed in Xenopus laevis oocytes, using the inside-out configurati
168 ce, and analysis of EAG currents recorded in Xenopus laevis oocytes, we show that a small molecule ch
172 ated MCT6 substrate/inhibitor specificity in Xenopus laevis oocytes; however, these data remain limit
178 e (FAK) as proteolytic targets of calpain in Xenopus laevis spinal cord neurons both in vivo and in v
181 y we examined the ability of pre-metamorphic Xenopus laevis tadpoles to self-correct malformed cranio
183 d an infection model system in the amphibian Xenopus laevis to study host responses to M. marinum at
184 t in ENaC isoforms of the aquatic pipid frog Xenopus laevis Using whole-cell and single-channel elect
185 revisiae Kar3, Saccharomyces pombe Pkl1, and Xenopus laevis XCTK2) are characterized by a C-terminal
187 Bs and those from Schizosaccharomyces pombe, Xenopus laevis, and Xenopus tropicalis formed stable hom
189 FP), hSGLT2-YFP and hSGLT3-YFP in oocytes of Xenopus laevis, injected hRS1-Reg(S20E), QEP, DFMO, and/
191 in Saccharomyces cerevisiae, C. elegans, and Xenopus laevis, we present studies identifying a novel d
196 so used mutagenesis and electrophysiology in Xenopus laevisoocytes to functionally map the determinan
197 establish that germ-line mutations in Kcp in Xenopus lead to valve defects and, ultimately, cardiac f
200 Finally, we tested patient variants in our Xenopus model and found the majority to be loss-of-funct
208 ing factor Ascl1 is injected directly into a Xenopus oocyte nucleus which has been preloaded with a l
211 describe how to use the technique for cells (Xenopus oocyte), tissues (Xenopus epithelium and rat cor
215 cterized 7 dicarboxylic acid transporters in Xenopus oocytes and in Saccharomyces cerevisiae engineer
216 high suppression potency in mammalian cells, Xenopus oocytes and mice in vivo, producing PTC repair i
217 i function is required for the maturation of Xenopus oocytes and specifically for translational activ
218 ese data indicate that H2S activates CFTR in Xenopus oocytes by inhibiting phosphodiesterase activity
219 and brain (alpha4beta2) nAChRs expressed in Xenopus oocytes by using a two-electrode voltage clamp a
221 duration current-voltage (I-V) protocol with Xenopus oocytes expressing eGFP-tagged NBCe1-A, our grou
222 cell and single-channel electrophysiology of Xenopus oocytes expressing ENaC isoforms assembled from
223 ked the compounds for rapid activation using Xenopus oocytes expressing human alpha7 nAChR with a two
224 l, as hyperpolarization of CNGC19-expressing Xenopus oocytes in the presence of both cyclic adenosine
225 maging conducted in murine retinal cells and Xenopus oocytes indicated that cell swelling in the phys
226 mistry and functional expression analysis in Xenopus oocytes indicates that the capacity of this H(+)
228 lectrode voltage clamp (TEVC) of transfected Xenopus oocytes revealed that the M2 S31N channel is ess
229 Electrophysiological characterization in Xenopus oocytes revealed that these derivatives differ i
230 kinases and because Nav1.7 had been shown in Xenopus oocytes to be affected by protein kinases C and
231 bunits and/or concatamers were injected into Xenopus oocytes to obtain receptors of defined subunit c
234 Functional Nav-LBT channels expressed in Xenopus oocytes were voltage-clamped, and distinct LRET
236 siological measurements in HEK-293 cells and Xenopus oocytes with pulldown experiments, we analyzed t
237 ethods, electrophysiological measurements in Xenopus oocytes, and fluorescent microscopy of mammalian
238 ionally following heterologous expression in Xenopus oocytes, and mediates both inward and outward tr
239 AQP0 were performed on protein expressed in Xenopus oocytes, and the results may therefore also refl
240 cal range activated TRPV4 in Muller glia and Xenopus oocytes, but required phospholipase A(2) (PLA(2)
241 displayed robust repair capacity, including Xenopus oocytes, Chlamydomonas, and Stentor coeruleus Al
242 arious heterologous cell expression systems (Xenopus oocytes, CHO cells, and rat atrial cardiomyocyte
243 ort function by expressing these proteins in Xenopus oocytes, Drip, Prip, and Eglp2 show significant
245 Using ion-selective microelectrodes and Xenopus oocytes, here we studied Cl(-)/H(+) coupling pro
248 K(2P) channels, expressed heterologously in Xenopus oocytes, were measured by two-electrode voltage
265 thin vertebrate vocal communication systems, Xenopus provides insights that can inform social communi
268 resent an analysis of all publicly available Xenopus RNA sequencing (RNA-seq) data in a reexamination
270 r transplantations from knockdown to control Xenopus showed that it is the Fzd3 expressed within the
277 enopus gills and mouse skin) and appendages (Xenopus tail), and provide recommendations on how to ada
281 present the cryo-EM structure of OTOP3 from Xenopus tropicalis (XtOTOP3) along with functional chara
282 ebrates, with two members in elephant shark, Xenopus tropicalis and Anolis lizard and three members i
283 Using the T3-dependent metamorphosis in Xenopus tropicalis as a model, we show here that high le
284 hizosaccharomyces pombe, Xenopus laevis, and Xenopus tropicalis formed stable homotetramers, the mtSS
286 the egg cytoplasm between Xenopus laevis and Xenopus tropicalis have been shown to account for spindl
287 rod photoreceptors in retinas of developing Xenopus tropicalis heterozygous, but not homozygous muta
289 ction of this gene, we used the diploid frog Xenopus tropicalis We discover that Dyrk1a is expressed
291 wo closely related frogs, Xenopus laevis and Xenopus tropicalis, have surprisingly different microtub
292 we show that the genetically tractable frog Xenopus tropicalis, paired with optical coherence tomogr
296 nic effects of aquatic exposure of Silurana (Xenopus) tropicalis embryos to commercial NA extracts an
299 s CRISPR/Cas9 approaches to target CTNND1 in Xenopus, we identified a subset of phenotypes that can b