ライフサイエンスコーパス: Y. enterocolitica

1 Y. enterocolitica biovar 1B additionally has a distinct

2 Y. enterocolitica cells are motile when grown at lower t

3 Y. enterocolitica demonstrated excellent 18F-FDS uptake

4 Y. enterocolitica infection promoted the development of

5 Y. enterocolitica infections in FoodNet sites have signi

6 Y. enterocolitica isolates recovered from the mice were

7 Y. enterocolitica lacking the virulence plasmid failed t

8 Y. enterocolitica mutants lacking either the Ysa or Ysc

9 Y. enterocolitica O:8 was isolated from 1 raw-milk sampl

10 Y. enterocolitica organisms were more virulent in the IL

11 Y. enterocolitica reduces S. Typhimurium invasion of HeL

12 Y. enterocolitica tends to persist in soil for long peri

13 Y. enterocolitica thus has three type III secretion path

14 Y. enterocolitica was also able to inhibit the invasion

15 Y. enterocolitica was present within the murine mucosa o

16 erence method was 1.2% ETEC, 0.1% Vibrio, 0% Y. enterocolitica, and 0% P. shigelloides Compared to th

17 Analysis of 100 Y. enterocolitica and Y. enterocolitica-like strains sho

18 From 1996 through 2009, 2085 Y. enterocolitica infections were reported to FoodNet.

19 he enteropathogenic E. coli strain E2348/69, Y. enterocolitica JB580, and Pseudomonas aeruginosa PAO1

20 .7% (99.4 to 99.8), and 0.96 (0.93 to 0.99); Y. enterocolitica, 99.0% (94.8 to 99.8), 99.9% (99.8 to

21 A Y. enterocolitica rovA mutant has a significant decrease

22 One role of IL-6 during a Y. enterocolitica infection may be the downmodulation of

23 In a Y. enterocolitica yenI mutant, swimming motility is temp

24 A striking feature of the pathology of a Y. enterocolitica infection is inflammation.

25 We previously reported the isolation of a Y. enterocolitica mutant (JB1A8v) that shows a decrease

26 of intestinal inflammation in response to a Y. enterocolitica infection.

27 Educating dairy owners about Y. enterocolitica and postpasteurization contamination i

28 After Y. enterocolitica challenge, YopB/LcrV+dmLT-vaccinated m

29 On day 14 after Y. enterocolitica infection (arthritis onset), we found

30 nt exhibited higher expression of CCR7 after Y. enterocolitica infection.

31 rt antipathogenic effects in the gut against Y. enterocolitica and highlight the need to investigate

32 The most potent MP against Y. enterocolitica, methicillin-resistant S. aureus and M

33 096 mg/L respectively) were observed against Y. enterocolitica.

34 Although Y. enterocolitica 0:8 strains are reported to have galac

35 een for Shiga toxin (Verigene EP, 89.3%) and Y. enterocolitica (Verigene EP, 88.9% and Fusion GI Bac,

36 common food pathogens, including E. coli and Y. enterocolitica, and could even detect Salmonella spp.

37 romotes serum resistance in both E. coli and Y. enterocolitica.

38 ose 4-epimerase activity in both E. coli and Y. enterocolitica.

39 Analysis of 100 Y. enterocolitica and Y. enterocolitica-like strains showed none to be within

40 , isolated 2,493 Yersinia enterocolitica and Y. enterocolitica-like strains, 22 Y. pestis strains, an

41 IV(A) modified with C16:0 predominated, and Y. enterocolitica produced a unique tetra-acylated lipid

42 ogenic for humans (Y. pseudotuberculosis and Y. enterocolitica).

43 ognate plasmids in Y. pseudotuberculosis and Y. enterocolitica, but their localization within the pla

44 umans (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) export and translocate a distinct set

45 sent in all 47 low-pathogenicity strains and Y. enterocolitica 8081 but absent from all nonpathogenic

46 occurring in response to S. typhimurium and Y. enterocolitica colonization of PP using Affymetrix Ge

47 l complementation of both S. typhimurium and Y. enterocolitica mutations and by DNA sequence analysis

48 hort interactions between S. Typhimurium and Y. enterocolitica or that the SdiA regulon members do no

49 rd to host cell uptake of S. Typhimurium and Y. enterocolitica, we investigated how each pathogen inf

50 However, because Y. enterocolitica is typically a food-borne pathogen, th

51 nt PspC destabilization is conserved between Y. enterocolitica and E. coli.

52 onserved mechanism of inv regulation between Y. enterocolitica and Y. pseudotuberculosis.

53 hich is positively regulated by RovA in both Y. enterocolitica and Yersinia pseudotuberculosis while

54 The RepA proteins encoded by Y. enterocolitica serotype 0:8 pYVeWA and pYVe8081 were

55 here early precursor B cells are expanded by Y. enterocolitica porins to undergo somatic hypermutatio

56 eveal the complete set of genes expressed by Y. enterocolitica in response to infection and provide t

57 ed to reverse the uptake blockade imposed by Y. enterocolitica.

58 erwise unable to block type III injection by Y. enterocolitica strains.

59 ntal signals are processed and integrated by Y. enterocolitica to stimulate the production of flagell

60 N-acylhomoserine lactones (AHLs) produced by Y. enterocolitica and upregulates the expression of an i

61 CopN, but not NrdB, was secreted by Y. enterocolitica in a Ca2+- and pYV-dependent fashion.

62 rison of Ysps with Yop effectors secreted by Y. enterocolitica indicated that YspG, YspH, and YspJ ha

63 the range of 30 degrees C and 37 degrees C, Y. enterocolitica phase-varies between motility and plas

64 ears to be provided by host cells and causes Y. enterocolitica to transport YopE and presumably other

65 were stimulated with flagella from E. coli, Y. enterocolitica, and P. aeruginosa in the presence of

66 active surveillance for laboratory-confirmed Y. enterocolitica infections, defined as the isolation o

67 e H(1) receptor is important for controlling Y. enterocolitica infection within the Peyer's patches a

68 ase TaqMan PCR assay was developed to detect Y. enterocolitica in blood.

69 degrees C was also derepressed in a directed Y. enterocolitica clpP mutant.

70 ting protective roles for CD4 T cells during Y. enterocolitica infection, vaccinating mice with a 16-

71 bitory role of this endogenous lectin during Y. enterocolitica infection.

72 ith a regulatory role for this lectin during Y. enterocolitica pathogenesis, mice lacking Gal-1 showe

73 IL-6 plays an anti-inflammatory role during Y. enterocolitica infection, and in other systems IL-6 h

74 A gene encoding Y. enterocolitica phospholipase was identified, and anal

75 as medium values decreased below pH 3.0 for Y. enterocolitica and pH 5.5 for M. morganii, suggesting

76 ally informative typing scheme available for Y. enterocolitica.

77 that the RovA regulon may be dispensable for Y. enterocolitica systemic disease and inflammatory resp

78 Establishment of S2 cells as a model for Y. enterocolitica infection provides a versatile tool to

79 e cAMP-CRP regulatory system is required for Y. enterocolitica virulence.

80 ne identified along with inv in a screen for Y. enterocolitica genes that could confer an invasive ph

81 Analysis of the core gene set for Y. enterocolitica revealed that 20.8% of the genes were

82 ucleotide sequence of the 16S rRNA gene from Y. enterocolitica were designed.

83 y factors Vn and C4BP, and Ail homologs from Y. enterocolitica and Y. pseudotuberculosis recruit fact

84 4, with and without this bend, isolated from Y. enterocolitica were resolved by using chloroquine gel

85 stis KIM D27 LcrV (LcrV(D27)) bind LcrV from Y. enterocolitica O:9 strain W22703 (LcrV(W22703)) or O:

86 We also show that RovA and H-NS from Y. enterocolitica bind to a similar region of the inv pr

87 activity, we have characterized the OGL from Y. enterocolitica, YeOGL, on oligogalacturonides and det

88 direct the secretion of an Npt reporter from Y. enterocolitica, indicating that a universal targeting

89 An aspirate from the axillary mass grew Y. enterocolitica.

90 These results reveal facets of how Y. enterocolitica controls the function of the Ysa TTS s

91 These findings add a new aspect of how Y. enterocolitica effectively evades the host complement

92 However, Y. enterocolitica had no effect on S. Typhimurium uptake

93 Yersinia bercovieri, a recently identified Y. enterocolitica-like species, produces a heat-stable e

94 In Y. enterocolitica, a rovA mutant is attenuated for virul

95 erefore, we investigated the role of ArcB in Y. enterocolitica and E. coli.

96 Mutating all CSC-boxes in Y. enterocolitica of a plasmid bound cspA1/A2 dramatical

97 se (18F-FDG) PET signal was not different in Y. enterocolitica colonized versus uncolonized tumors.

98 y, when YspP was constitutively expressed in Y. enterocolitica bv.

99 g mechanism for virulence gene expression in Y. enterocolitica and other enteric pathogens.

100 ositive regulators of psp gene expression in Y. enterocolitica.

101 d the gene for the lipoprotein YlpA found in Y. enterocolitica likely is a pseudogene in Y. pestis.

102 ia coli galE mutant, its primary function in Y. enterocolitica is not in the production of UDP galact

103 Furthermore, in Y. enterocolitica RovM only in the presence of Hfq affec

104 a CSC-box into a plasmid-bound lacZ gene in Y. enterocolitica, the mRNA of this construct was cleave

105 noyl)-l-homoserine lactone (3-oxo-C6-HSL) in Y. enterocolitica and inhibit QS-associated biofilm matu

106 ditional galE homolog has been identified in Y. enterocolitica by homology to the E. coli gene.

107 pholipase YplA, which has been implicated in Y. enterocolitica virulence.

108 tion that a number of RovA-regulated loci in Y. enterocolitica do not have orthologues in Y. pestis a

109 logues in Yersinia pestis plasmid pCD1 or in Y. enterocolitica serotype 0:9 plasmid pYVe227.

110 endogenous chromosomally encoded proteins in Y. enterocolitica revealed discrete complexes correspond

111 FtsH destabilizes PspC in Y. enterocolitica, but coproduction of PspC with its bin

112 the membrane topologies of PspB and PspC in Y. enterocolitica.

113 r to alleviate transcriptional repression in Y. enterocolitica.

114 olog has been demonstrated to have a role in Y. enterocolitica serotype 0:8 O-polysaccharide antigen

115 entified a new positive regulator of rovA in Y. enterocolitica, LeuO.

116 nal response to prolonged secretin stress in Y. enterocolitica.

117 oli, Salmonella, and Yersinia pestis than in Y. enterocolitica.

118 s KIM6+ system is most homologous to that in Y. enterocolitica, showing identities of 84% for YfuA (p

119 le in the modulation of ail transcription in Y. enterocolitica.

120 ulosis while negatively regulated by YmoA in Y. enterocolitica and H-NS in Y. pseudotuberculosis.

121 pseudotuberculosis and Y. pestis and YopP in Y. enterocolitica has been shown to regulate host immune

122 been linked to robust phenotypes, including Y. enterocolitica virulence.

123 le of targeting YopP and that they influence Y. enterocolitica interactions with macrophages.

124 +dmLT achieved 60% protection against lethal Y. enterocolitica infection, and vaccine efficacy increa

125 ed modest (10-30%) protection against lethal Y. enterocolitica oral infection.

126 fices to protect against an otherwise lethal Y. enterocolitica challenge.

127 dendritic cells, and a yopP mutant of a live Y. enterocolitica carrier vaccine elicited effective pri

128 eless, the ytxAB genes are conserved in many Y. enterocolitica strains.

129 In adult mice, Y. enterocolitica rapidly disseminated to the spleen and

130 Unlike S. typhimurium flgM mutants, Y. enterocolitica flgM mutants are fully virulent.

131 genes in response to S. typhimurium but not Y. enterocolitica.

132 Subsequently, a yplA-null Y. enterocolitica strain, YEDS10, was constructed and de

133 at the Ysa TTS system impacts the ability of Y. enterocolitica to colonize gastrointestinal tissues.

134 In this study, we tested the ability of Y. enterocolitica to modulate intracellular IL-1alpha-de

135 uction of gut inflammation characteristic of Y. enterocolitica infection.

136 Using a genetic approach, a collection of Y. enterocolitica Ysa TTS mutants was generated by mutag

137 and inflammatory cytokines in the control of Y. enterocolitica infection in IL-6(-/-) mice was undert

138 he role of the Ysps during the life cycle of Y. enterocolitica.

139 ion of YscM1 and YscM2 from the cytoplasm of Y. enterocolitica causes an increase in yop expression,

140 potential for use in the rapid detection of Y. enterocolitica contamination in stored blood units.

141 ors are conserved in Yersinia, divergence of Y. enterocolitica and Y. pseudotuberculosis/Y. pestis du

142 evidence that it is the C-terminal domain of Y. enterocolitica PspC (PspC(CT)) that interacts directl

143 initial examination of the effectiveness of Y. enterocolitica cya and crp mutants to stimulate prote

144 common with the heat-stable enterotoxins of Y. enterocolitica (YST I and YST II), it appears to be a

145 Examination of Y. enterocolitica-infected J774A.1 macrophages revealed

146 The ail gene of Y. enterocolitica is regulated by temperature and growth

147 The pspC gene of Y. enterocolitica was found to be important for normal g

148 gulates potentially novel virulence genes of Y. enterocolitica during infection.

149 oteases in a screen for chromosomal genes of Y. enterocolitica that were exclusively expressed during

150 re unable to block the type III injection of Y. enterocolitica strains, expression of lcrV(W22703) or

151 tica infections, defined as the isolation of Y. enterocolitica or unspeciated Yersinia from a human c

152 In this study, the psp locus of Y. enterocolitica was characterized further.

153 In addition, motility of Y. enterocolitica is regulated by temperature.

154 s indicate that an inv yadA double mutant of Y. enterocolitica is avirulent while an inv yadA mutant

155 through mice infected with a yenI mutant of Y. enterocolitica that cannot synthesize AHLs.

156 we screened transposon insertion mutants of Y. enterocolitica W22703 for defects in type III secreti

157 samples were spiked with various numbers of Y. enterocolitica cells, and total chromosomal DNA was e

158 tinct from the flagella secretion pathway of Y. enterocolitica.

159 mporally dynamic gene expression patterns of Y. enterocolitica biovar 1B through the course of an in

160 it secretes (Yops), prevents phagocytosis of Y. enterocolitica and is required for disease processes

161 he N-acylhomoserine lactone (AHL) profile of Y. enterocolitica.

162 ctly and specifically to the inv promoter of Y. enterocolitica.

163 to change the incompatibility properties of Y. enterocolitica serotype 0:8 plasmids from those of Y.

164 nv, the gene encoding the invasin protein of Y. enterocolitica, hreP is located in a cluster of flage

165 in this study we show that PspB and PspC of Y. enterocolitica are dual function proteins, acting bot

166 YadA, which is the primary C4BP receptor of Y. enterocolitica.

167 little overlap between the RovA regulons of Y. enterocolitica and Y. pestis despite the fact that Ro

168 single factor mediating serum resistance of Y. enterocolitica, presumably by binding C4b binding pro

169 d CD103(+)CD11b(+)) were found in the RLN of Y. enterocolitica-infected TNFRp55(-/-) mice.

170 Sequence analysis of the JB580v strain of Y. enterocolitica shows that, due to a premature stop co

171 A ure mutant strain of Y. enterocolitica was constructed which was hypersensiti

172 tigate a diverse collection of 94 strains of Y. enterocolitica consisting of 35 human, 35 pig, 15 she

173 Nonmotile strains of Y. enterocolitica were less invasive than motile strains

174 ase for the existence of three subspecies of Y. enterocolitica.

175 is apparently essential for the survival of Y. enterocolitica during infection.

176 ed as being required for in vivo survival of Y. enterocolitica.

177 pendent and distantly related TTS systems of Y. enterocolitica recognize protein substrates by a simi

178 tein (invasin(pstb)) was compared to that of Y. enterocolitica invasin (invasin(ent)), which lacks th

179 colitica serotype 0:8 plasmids from those of Y. enterocolitica serotype 0:9 and Y. pestis LCR plasmid

180 uctural subunits is most similar to those of Y. enterocolitica urease.

181 the development and clinical translation of Y. enterocolitica-based tumor-targeting bacterial therap

182 Fingolimod (FTY720) treatment of Y. enterocolitica-infected mice reduced the CD11b(-) sub

183 ave opened with the discovery of the Ysps of Y. enterocolitica Biovar 1B, which are translocated into

184 vitro FTY720 treatment downregulated CCR7 on Y. enterocolitica-infected bone marrow-derived DCs and p

185 a 17kDa cell-surface protein that confers on Y. enterocolitica resistance to serum killing and the ab

186 escribed here compare oral S. typhimurium or Y. enterocolitica infection in stromelysin-1 (MMP-3)-def

187 es of 7-day-old and adult mice to orogastric Y. enterocolitica infection were assessed in 50% lethal

188 rium is primarily an intracellular pathogen, Y. enterocolitica survives primarily extracellularly.

189 n the pathogenic Yersiniae (Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis).

190 al for protection of neonates during primary Y. enterocolitica infection.

191 To determine how temperature regulates Y. enterocolitica motility, we have been dissecting the

192 To subvert the host's immune response, Y. enterocolitica uses a type III secretion system consi

193 tructed for four hre genes and the resulting Y. enterocolitica mutants were tested in the mouse model

194 common to three pathogenic Yersinia species: Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis.

195 plasmids are from a common ancestor but that Y. enterocolitica serotype 0:8 plasmid replicons may hav

196 ults, biochemical evidence demonstrated that Y. enterocolitica and M. morganii ureases were activated

197 We show here that Y. enterocolitica is capable of infecting S2 cells and r

198 Overall, these studies support the idea that Y. enterocolitica promotes the development of highly inf

199 Previously, we reported that Y. enterocolitica PspB functions to positively control t

200 Further analysis revealed that Y. enterocolitica does not cluster according to source (

201 Here we show that Y. enterocolitica polymerizes a 6-kDa protein of the sec

202 ding model of factor H to YadA and show that Y. enterocolitica YadA recruits C3b and iC3b directly, w

203 In this study, we showed that Y. enterocolitica serotype O8 survives buffered acidic c

204 These data suggest that Y. enterocolitica inhibits intracellular pre-IL-1alpha s

205 Together these findings suggest that Y. enterocolitica produces a phospholipase A which has a

206 The Y. enterocolitica ClpB homologue is 30 to 40% identical

207 The Y. enterocolitica clpP gene complemented the clpP mutant

208 The Y. enterocolitica fliA gene, encoding the flagellar-spec

209 The Y. enterocolitica psp locus is made up of two divergentl

210 The Y. enterocolitica pspG gene was identified because its p

211 Comparisons between the Y. enterocolitica sif genes and the previously identifie

212 ogene, which occurs as an intact gene in the Y. enterocolitica and Y. pseudotuberculosis-derived anal

213 nd ytxAB genes are not closely linked in the Y. enterocolitica chromosome, and whereas ytxR is presen

214 t H-NS and RovA bind is not conserved in the Y. enterocolitica promoter.

215 otype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were id

216 responsible for the unique properties of the Y. enterocolitica and M. morganii ureases since the L. f

217 The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had simil

218 Identification of the Y. enterocolitica fliA and flgM genes was accomplished b

219 1122 recombined with a close relative of the Y. enterocolitica phage phiYeO3-12 to yield progeny phag

220 refore, PspG is the missing component of the Y. enterocolitica Psp regulon that was previously predic

221 We also compared induction of the Y. enterocolitica Psp, RpoE, and Cpx responses.

222 al regulator that controls expression of the Y. enterocolitica ytxAB genes.

223 These data suggest that the Y. enterocolitica pYV plasmid may undergo a conformation

224 nt protein reporters, we determined that the Y. enterocolitica rovA (rovA(Yent)) promoter is weaker t

225 Here, we present evidence that the Y. enterocolitica virulence plasmid, pYV, undergoes a co

226 Even though Y. enterocolitica induces a robust inflammatory response

227 f the bacteria to colonize neonatal tissues; Y. enterocolitica was readily detectable in the intestin

228 est that the Ysa and Ysc TTSSs contribute to Y. enterocolitica virulence by exporting both unique and

229 expressed in mucosal tissues, contributes to Y. enterocolitica pathogenicity by undermining protectiv

230 ll responses elicited in neonates exposed to Y. enterocolitica were associated with long-term protect

231 d obtained from mouse antiserum generated to Y. enterocolitica.

232 nfection, C57BL/6 mice are more resistant to Y. enterocolitica than are BALB/c mice.

233 as significantly up-regulated in response to Y. enterocolitica infection.

234 eful model for studying the host response to Y. enterocolitica infection.

235 r IFN-gamma that are produced in response to Y. enterocolitica.

236 f IL-1 alpha in mice infected with wild-type Y. enterocolitica results in significantly decreased int

237 ly 10-fold higher than that of the wild-type Y. enterocolitica, and there are significant inflammator

238 a yopP-deficient strain than with wild-type Y. enterocolitica, whereas only modest increases occurre

239 es compared to those infected with wild-type Y. enterocolitica.

240 eristics previously described following i.v. Y. enterocolitica infection.

241 l methods for the detection of ETEC, Vibrio, Y. enterocolitica, and P. shigelloides in stool specimen

242 obtained using two different high-virulence Y. enterocolitica strains.

243 of neonatal mice with low doses of virulent Y. enterocolitica leads to vigorous intestinal and syste

244 f a spontaneously arising pathogenic Ab with Y. enterocolitica.

245 Infection of eukaryotic cells with Y. enterocolitica strains expressing a Ysp-CyaA chimeric

246 t was prevented when mice were infected with Y. enterocolitica lacking YopP or YopH, two critical eff

247 rvival of MMP-3-deficient mice infected with Y. enterocolitica when compared with littermate controls

248 of intestinal lymphatic tissue infected with Y. enterocolitica.

249 nteric lymph nodes after oral infection with Y. enterocolitica.

250 dose (LD(50)) following oral infection with Y. enterocolitica.

251 After i.p. inoculation with Y. enterocolitica, fibrinogen-deficient mice display imp

252 llowing either i.p. or i.v. inoculation with Y. enterocolitica.

253 es AHLs to mimic a constant interaction with Y. enterocolitica.

254 patches of mice infected orogastrically with Y. enterocolitica serotype O:8 compared with noninfected

255 A, OmpC, and OmpF confirming reactivity with Y. enterocolitica.

256 The role of microbial Ags was tested with Y. enterocolitica proteins.

257 gnificantly higher PET signal in tumors with Y. enterocolitica colonization compared to those not col