ライフサイエンスコーパス: Y. pseudotuberculosis

1 Y. pseudotuberculosis binding to cells caused robust rec

2 Y. pseudotuberculosis expressing wild-type YpkA, but not

3 Y. pseudotuberculosis forms clustered centers of replica

4 Y. pseudotuberculosis phoP mutants died at a low rate in

5 Y. pseudotuberculosis phoP mutants were unable to replic

6 Y. pseudotuberculosis strains also varied greatly in the

7 Y. pseudotuberculosis translocated to organs such as the

8 signature-tagged mutagenesis, we isolated 13 Y. pseudotuberculosis mutants that failed to survive in

9 Five of 18 Y. pseudotuberculosis strains, encompassing seven seroty

10 a-like strains, 22 Y. pestis strains, and 21 Y. pseudotuberculosis strains from 130,574 clinical and

11 A Y. pseudotuberculosis phoP mutant was 100-fold less viru

12 ly to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity s

13 ar, a translocation defect was observed in a Y. pseudotuberculosis strain that expressed an uptake-de

14 However, in a Y. pseudotuberculosis ypsI-negative background, YtbI app

15 ipid A, and an attenuated strain producing a Y. pseudotuberculosis-like hexa-acylated lipid A.

16 In addition, Y. pseudotuberculosis mutants expressing YopM proteins u

17 ed how host cell sterol composition affected Y. pseudotuberculosis uptake.

18 Decreases in NK cells after Y. pseudotuberculosis infection did not correlate with Y

19 Although Y. pseudotuberculosis makes biofilms in other environmen

20 stis isolates and its evolutionary ancestor, Y. pseudotuberculosis.

21 and Ail homologs from Y. enterocolitica and Y. pseudotuberculosis recruit factor H.

22 an intact gene in the Y. enterocolitica and Y. pseudotuberculosis-derived analogues, was found in pC

23 iption of inv in Yersinia enterocolitica and Y. pseudotuberculosis.

24 inv regulation between Y. enterocolitica and Y. pseudotuberculosis.

25 ersinia, divergence of Y. enterocolitica and Y. pseudotuberculosis/Y. pestis during evolution has res

26 iae (Yersinia pestis, Y. enterocolitica, and Y. pseudotuberculosis).

27 lla pneumoniae, Yersinia enterocolitica, and Y. pseudotuberculosis.

28 . pestis, the agent of plague in humans, and Y. pseudotuberculosis, the related enteric pathogen, del

29 lly, when antiphagocytosis is incomplete and Y. pseudotuberculosis is internalized by macrophages, th

30 present on the chromosomes of Y. pestis and Y. pseudotuberculosis and that this secretion system is

31 However, both Y. pestis and Y. pseudotuberculosis form biofilms that adhere to the e

32 Studies in Y. pestis and Y. pseudotuberculosis have shown that YopM suppresses in

33 te in macrophages is common to Y. pestis and Y. pseudotuberculosis or is a unique phenotype of Y. pes

34 A number of Y. pestis and Y. pseudotuberculosis strains of different biovars and s

35 macrophages compared to other Y. pestis and Y. pseudotuberculosis strains tested.

36 logous sequences from numerous Y. pestis and Y. pseudotuberculosis strains, we determined that these

37 ars, serotypes, and strains of Y. pestis and Y. pseudotuberculosis that may relate to the evolution o

38 We compared the ability of Y. pestis and Y. pseudotuberculosis to infect the rat flea Xenopsylla

39 n cleavage is specific for the Y. pestis and Y. pseudotuberculosis YapE orthologues but is not conser

40 hat biofilms produced by Yersinia pestis and Y. pseudotuberculosis, which bind the C. elegans surface

41 in macrophages is conserved in Y. pestis and Y. pseudotuberculosis.

42 acquisition of this island by Y. pestis and Y. pseudotuberculosis.

43 in-specific manner and only in Y. pestis and Y. pseudotuberculosis.

44 ops do not completely block phagocytosis and Y. pseudotuberculosis can replicate in macrophages, it i

45 m and on wild-type Y. pseudotuberculosis and Y. pseudotuberculosis QS mutants.

46 infections by Yersinia enterocolitica WA and Y. pseudotuberculosis PB1+.

47 Six sRNAs are Y. pseudotuberculosis specific and are absent from the g

48 ght junctions) to the apical compartment, as Y. pseudotuberculosis cells lacking the inv gene were un

49 est that it is possible to use an attenuated Y. pseudotuberculosis strain delivering the LcrV antigen

50 e expression of many sRNAs conserved between Y. pseudotuberculosis and Y. pestis differs in both timi

51 perons had high levels of divergence between Y. pseudotuberculosis strains.

52 This suggests that both Y. pseudotuberculosis and Y. pestis produce an oligosacc

53 eading to increased systemic colonization by Y. pseudotuberculosis and potentially enhancing adaptive

54 effects of spleen and liver colonization by Y. pseudotuberculosis on yop mutants in the intestines.

55 that three virulence determinants encoded by Y. pseudotuberculosis manipulate the Rho GTPase RhoG.

56 ork also indicates that biofilm formation by Y. pseudotuberculosis on C. elegans is an interactive pr

57 ty regulator, FlhDC, in biofilm formation by Y. pseudotuberculosis on C. elegans.

58 used to show efficient RhoG inactivation by Y. pseudotuberculosis YopE, a potent Rho GTPase activati

59 monolayers became susceptible to invasion by Y. pseudotuberculosis.

60 We show that the cell death mediated by Y. pseudotuberculosis is apoptosis.

61 Here we show that Hfq is required by Y. pseudotuberculosis to cause mortality in an intragast

62 he global set of sRNAs expressed in vitro by Y. pseudotuberculosis.

63 itment of both fH and C4BP by Ail may confer Y. pseudotuberculosis with the ability to resist all pat

64 In contrast, Y. pseudotuberculosis strains which did not contain IS10

65 As the YopD(DeltaTM) Y. pseudotuberculosis mutant formed somewhat smaller por

66 val of pYV(+) but not pYV-cured or DeltayopB Y. pseudotuberculosis within phagosomes: only a small fr

67 l assay (type III secretion system dependent Y. pseudotuberculosis cytotoxicity assay).

68 hogenic Yersinia species: Y. enterocolitica, Y. pseudotuberculosis, and Y. pestis.

69 ust immune response against enteropathogenic Y. pseudotuberculosis by promoting Th17-like responses i

70 , the GAP function of YopE was important for Y. pseudotuberculosis pathogenesis in a mouse infection

71 replication in macrophages are important for Y. pseudotuberculosis pathogenesis.

72 Here the importance of PhoP for Y. pseudotuberculosis pathogenesis was investigated.

73 Therefore, YopJ is required for Y. pseudotuberculosis to downregulate MAP kinases and in

74 An increased risk for Y. pseudotuberculosis infection was specifically related

75 we therefore propose an infection route for Y. pseudotuberculosis where this pathogen, after penetra

76 E and PNPase can be readily copurified from Y. pseudotuberculosis cell extracts, suggests that these

77 s is similar to recently published data from Y. pseudotuberculosis, revealing a potentially conserved

78 ear which of these phenotypes descended from Y. pseudotuberculosis and which were acquired independen

79 estis acquired since the the divergence from Y. pseudotuberculosis.

80 Yersinia pestis evolved from Y. pseudotuberculosis to become the causative agent of b

81 PCP1 during the divergence of Y. pestis from Y. pseudotuberculosis, and are the first evidence of a n

82 cement of the pseudogene with the functional Y. pseudotuberculosis rcsA allele strongly represses bio

83 he contribution of this region by generating Y. pseudotuberculosis yopD(Delta150-170) and yopD(Delta2

84 During aerobic growth on glucose, Y. pseudotuberculosis reveals an unusual flux distributi

85 IV(A) modified with C10 or C12 acyl groups, Y. pseudotuberculosis contained the same forms as part o

86 two Yersinia species pathogenic for humans (Y. pseudotuberculosis and Y. enterocolitica).

87 In Y. pseudotuberculosis, rovA transcription is controlled

88 ith a hierarchical quorum-sensing cascade in Y. pseudotuberculosis that is involved in the regulation

89 YPTB3286), we show that yapK is conserved in Y. pseudotuberculosis, while yapJ is unique to Y. pestis

90 evealing potential virulence determinants in Y. pseudotuberculosis that are regulated in a posttransc

91 regulator of biofilms that is functional in Y. pseudotuberculosis, is a pseudogene in Y. pestis.

92 xpression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolis

93 Disruption of the ail gene in Y. pseudotuberculosis resulted in loss of C4BP binding.

94 ver, the deletion of the homologous genes in Y. pseudotuberculosis does not seem to impact the virule

95 g yapK and yapJ to three homologous genes in Y. pseudotuberculosis IP32953 (YPTB0365, YPTB3285, and Y

96 mophagocyte in this disease model but not in Y. pseudotuberculosis-infected mice.

97 tion at 37 degrees C in Y. pestis but not in Y. pseudotuberculosis.

98 ted by YmoA in Y. enterocolitica and H-NS in Y. pseudotuberculosis.

99 Conversely, deletion of the urease operon in Y. pseudotuberculosis rendered it nontoxic.

100 mologous to those of the cognate plasmids in Y. pseudotuberculosis and Y. enterocolitica, but their l

101 stem (TTSS) found in Y. pestis is present in Y. pseudotuberculosis and whether this system is importa

102 he hms genes; identical genes are present in Y. pseudotuberculosis.

103 okine CCL28 was increased by loss of rfaH in Y. pseudotuberculosis but not in Y. pestis.

104 Deletion of multiple sRNAs in Y. pseudotuberculosis leads to attenuation of the pathog

105 Because a key step in Y. pseudotuberculosis internalization is interaction of

106 Primer extension analyses indicate that, in Y. pseudotuberculosis, the transcription of the psaE and

107 with these results, IL-10 is undetectable in Y. pseudotuberculosis-infected mouse tissues until advan

108 Virulence in Y. pseudotuberculosis requires the plasmid-encoded Ysc t

109 I secretion system effector known as YopJ in Y. pseudotuberculosis and Y. pestis and YopP in Y. enter

110 ng enzymatic activities of YopE and YopT, in Y. pseudotuberculosis-infected macrophages.

111 er the T3SS can be utilized by intracellular Y. pseudotuberculosis to translocate Yops.

112 t was protective against lethal intragastric Y. pseudotuberculosis challenge.

113 Last, Y. pseudotuberculosis did not require Ivy to counter lys

114 ted the macrophage cytokine response to live Y. pseudotuberculosis and analyzed the susceptibility of

115 attempt to reduce binding to luminal mucus, Y. pseudotuberculosis yadA and inv yadA strains were ana

116 strate the misregulation of RhoG by multiple Y. pseudotuberculosis virulence determinants.

117 Mutant Y. pseudotuberculosis that do not make any Yop proteins

118 acrophages infected with wild-type or mutant Y. pseudotuberculosis strains.

119 Phagosomes containing phoP mutant Y. pseudotuberculosis acquired cathepsin D at a higher r

120 phagosomes containing phoP(+) or phoP mutant Y. pseudotuberculosis was studied by using immunofluores

121 pYV-cured, pYV(+) wild-type, and yop mutant Y. pseudotuberculosis strains were allowed to infect bon

122 intravenously with wild-type or yopM mutant Y. pseudotuberculosis strains.

123 detected, indicating that as many as 13% of Y. pseudotuberculosis genes no longer function in Y. pes

124 The ability of Y. pseudotuberculosis to promote apoptosis of macrophage

125 ars intimately connected with the ability of Y. pseudotuberculosis to successfully establish replicat

126 Transcriptomic analysis of Y. pseudotuberculosis revealed that genes located in bot

127 ranasal inoculation with as few as 18 CFU of Y. pseudotuberculosis caused a lethal lung infection in

128 nds inhibited T3SS-dependent cytotoxicity of Y. pseudotuberculosis toward macrophages in vitro.

129 The sterol structure dependence of Y. pseudotuberculosis internalization differed from that

130 th in the intestine delayed dissemination of Y. pseudotuberculosis.

131 id containing a 6.7-kb KpnI-ClaI fragment of Y. pseudotuberculosis encompassing the psa locus was suf

132 ia pestis and pesticin-sensitive isolates of Y. pseudotuberculosis possess a common 34 kbp DNA region

133 In an orogastric model of Y. pseudotuberculosis infection, a Delta yopM mutant was

134 In a murine model of Y. pseudotuberculosis pneumonia, two compounds significa

135 Furthermore, the Deltahfq mutant of Y. pseudotuberculosis is hypermotile and displays increa

136 ica is avirulent while an inv yadA mutant of Y. pseudotuberculosis is hypervirulent.

137 ersinia, we constructed DeltarfaH mutants of Y. pseudotuberculosis IP32953 and Y. pestis KIM6+.

138 New constructs of the inv yadA mutants of Y. pseudotuberculosis were made and tested in mice.

139 es and prepared lpp gene deletion mutants of Y. pseudotuberculosis YPIII, Y. pestis KIM/D27 (pigmenta

140 characterized a glycosyl hydrolase (NghA) of Y. pseudotuberculosis that cleaved beta-linked N-acetylg

141 regions were specific to a limited number of Y. pseudotuberculosis strains, including the high pathog

142 bacterium and host following phagocytosis of Y. pseudotuberculosis suggests new roles for the T3SS in

143 T3SS expression levels among a population of Y. pseudotuberculosis cells following the removal of Ca(

144 Random screening of Y. pseudotuberculosis transposon insertion mutants with

145 , we report the complete genomic sequence of Y. pseudotuberculosis IP32953 and its use for detailed g

146 th either the IP32953 or the 32777 strain of Y. pseudotuberculosis, demonstrating that the phenotype

147 of 22 strains of Y. pestis and 10 strains of Y. pseudotuberculosis of diverse origin.

148 atalytically inactive yopJ mutant strains of Y. pseudotuberculosis was developed to further investiga

149 absent or highly divergent in all strains of Y. pseudotuberculosis.

150 molecular marker that identifies a subset of Y. pseudotuberculosis isolates that have a particularly

151 e for Fe-S cluster repair in the survival of Y. pseudotuberculosis within the spleen and suggest that

152 ly, our results suggest that the Ysc T3SS of Y. pseudotuberculosis can function within macrophage pha

153 Through the use of Y. pseudotuberculosis mutants, we show that the inhibito

154 fq plays a critical role in the virulence of Y. pseudotuberculosis by participating in the regulation

155 e in allowing adhesion of M. nematophilum or Y. pseudotuberculosis biofilm to wild type C. elegans.

156 Though the closely related enteric pathogen Y. pseudotuberculosis uses quorum sensing system to regu

157 y evolved from the gastrointestinal pathogen Y. pseudotuberculosis; however, it is not known at what

158 ia species pathogenic for humans (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) export and

159 fy the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica

160 phoP(+) Y. pseudotuberculosis strains initiated replication in m

161 tants, suggesting that YopE and YopH protect Y. pseudotuberculosis from these defenses in BALB/c mice

162 e macrophage response to internalized pYV(+) Y. pseudotuberculosis.

163 f macrophages harboring intracellular pYV(+) Y. pseudotuberculosis with chloramphenicol reduced apopt

164 f macrophages infected with wild-type pYV(+) Y. pseudotuberculosis died of apoptosis after 20 h.

165 Y. pestis and the closely related Y. pseudotuberculosis also block the feeding of Caenorha

166 of plague, from the closely related species Y. pseudotuberculosis.

167 Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspa

168 In this article, we demonstrate that Y. pseudotuberculosis Ail from strains PB1, 2812/79, and

169 In this study, we demonstrate that Y. pseudotuberculosis utilizes its lipopolysaccharide (L

170 These results indicate that Y. pseudotuberculosis adhesive factors control the site

171 These results indicate that Y. pseudotuberculosis Ail recruits C4BP in a functional

172 Fluorescence localization indicated that Y. pseudotuberculosis selectively associated with monola

173 o Yops have some redundant functions or that Y. pseudotuberculosis employs multiple strategies for co

174 Here we report that Y. pseudotuberculosis cells with reduced RNase E activit

175 erial cell binding and entry mediated by the Y. pseudotuberculosis invasin protein (invasin(pstb)) wa

176 To determine the Y. pseudotuberculosis factors important for growth durin

177 These changes from the Y. pseudotuberculosis progenitor included loss of insect

178 the intestinal mucosal membrane, hijacks the Y. pseudotuberculosis-CD209 interaction antigen-presenti

179 Mutations in the Y. pseudotuberculosis ail and psa loci were constructed

180 ort a model in which a genetic change in the Y. pseudotuberculosis progenitor of Y. pestis extended i

181 he C-terminal 192-residue superdomain of the Y. pseudotuberculosis invasin is necessary and sufficien

182 orm from the cell by the introduction of the Y. pseudotuberculosis YopE RhoGAP protein could be bypas

183 The blocking of the Y. pseudotuberculosis-CD209 interactions by expression o

184 ed sensitivity to host defense peptides, the Y. pseudotuberculosis DeltarfaH strain was not attenuate

185 Only one of six strains tested, the Y. pseudotuberculosis YPIII(p(-)) strain, was defective

186 ovA (rovA(Yent)) promoter is weaker than the Y. pseudotuberculosis promoter.

187 luding those phylogenetically closest to the Y. pseudotuberculosis progenitor, contain a mutated ureD

188 and RovA bind with a higher affinity to the Y. pseudotuberculosis/Y. pestis rovA (rovA(Ypstb/Ypestis

189 Therefore, Y. pseudotuberculosis subverts intestinal barrier functi

190 These Y. pseudotuberculosis-CD209 interactions result in bacte

191 Three Y. pseudotuberculosis factors, previously identified by

192 estis strains (EV766 and KIM10(+)) and three Y. pseudotuberculosis strains (IP2790c, IP2515c, and IP2

193 ant produced similar levels of antibodies to Y. pseudotuberculosis antigens and were equally resistan

194 rtant mechanism by which YopM contributes to Y. pseudotuberculosis virulence.

195 monstrate that after a transient exposure to Y. pseudotuberculosis, T and B cells are impaired in the

196 optotic response of TLR2(-/-) macrophages to Y. pseudotuberculosis infection is equivalent to that of

197 ence and absence of biofilm and on wild-type Y. pseudotuberculosis and Y. pseudotuberculosis QS mutan

198 ine macrophages were infected with wild-type Y. pseudotuberculosis but not with an isogenic ysc mutan

199 spleens showed that infection with wild-type Y. pseudotuberculosis caused an influx of neutrophils, w

200 Infection with wild-type Y. pseudotuberculosis caused significantly more inflamma

201 To determine how wild-type Y. pseudotuberculosis hinders yop mutant survival, yop m

202 that isolated specific features of wild-type Y. pseudotuberculosis infection.

203 Wild-type Y. pseudotuberculosis kills approximately 70% of infecte

204 Over the course of 7 days, wild-type Y. pseudotuberculosis replicated to nearly 1 x 10(8) CFU

205 Infection of C. elegans with the wild-type Y. pseudotuberculosis resulted in the differential regul

206 ail to persist in competition with wild-type Y. pseudotuberculosis, indicating that these two infecti

207 In competition infections with wild-type Y. pseudotuberculosis, the presence of wild-type bacteri

208 epithelial cells as efficiently as wild-type Y. pseudotuberculosis.

209 In addition, using Y. pseudotuberculosis strains marked with signature tags

210 This possibility was investigated here using Y. pseudotuberculosis strains that express YopJ or YopH

211 ld be correlated with the presence of viable Y. pseudotuberculosis in macrophages.

212 is no consensus in the literature on whether Y. pseudotuberculosis shares this property.

213 e compared the sterol dependence of wildtype Y. pseudotuberculosis internalization with that of Delta

214 efold in TLR4(-/-) macrophages infected with Y. pseudotuberculosis, while the apoptotic response of T

215 ions in human epithelial cells infected with Y. pseudotuberculosis.

216 poptosis (YopJ) in macrophages infected with Y. pseudotuberculosis.

217 In summary, infection with Y. pseudotuberculosis in intestinal and systemic sites i

218 the response) during primary infection with Y. pseudotuberculosis, as shown by flow cytometry tetram

219 Results of infections with Y. pseudotuberculosis expressing catalytically inactive

220 Finally, competition infections with Y. pseudotuberculosis mutants with various abilities to

221 Furthermore, this lung infection model with Y. pseudotuberculosis can be used to test potential ther

222 ma) in serum samples of mice vaccinated with Y. pseudotuberculosis.

223 killing of macrophages infected ex vivo with Y. pseudotuberculosis, suggesting a mechanism by which t

224 y, HeLa cells infected with wild-type or Yop-Y. pseudotuberculosis strains were assayed for interleuk