ライフサイエンスコーパス: YAC

1 YAC d792t2 restored senescence in both human and rat mam

2 YAC DNA present in A9/YAC hybrids was further transferre

3 YAC targeting technology (YTT) uses the WIBR/MIT-820 C57

4 YACs mapped with respect to deletion breakpoints localiz

5 YACs up to 670 kb can be efficiently circularized using

6 033 from our own data, and a total of 56,093 YACs, of which 44,401 are positive for more than one mar

7 y high-resolution cytogenetics, FISH with 19 YACs, and PCR using 25 different sequence-tagged sites.

8 patterns of shared markers in a panel of 21 YAC clones.

9 This contig was constructed from 24 YACs, 34 BACs, and 1 P1 phage clone onto which 71 marker

10 ne was first inserted into both large GATA-3 YACs.

11 AC/ STS map of this region that includes 436 YACs anchored by 216 STSs.

12 A YAC/BAC contig that gives continuous coverage between PL

13 A YAC/BAC contig was assembled by STS content mapping and

14 To identify candidate TSGs on 8p22 a YAC contig spanning this region was assembled and YAC cl

15 tween the markers D12S377 and D12S296, and a YAC clone contig covering the region was generated.

16 the previously constructed genetic map and a YAC-based physical map reported in a companion paper, th

17 expression, we isolated human SLC10A1 from a YAC chromosomal clone.

18 Deletion of this enhancer from a YAC reporter construct that recapitulates the Myf5 expre

19 that contains elements needed to generate a YAC (cen4, ars, ura3, his, and two telomere segments) al

20 ditional RAD52 gene that allow transfer of a YAC from any host into a recombination-deficient backgro

21 malian DNA results in the establishment of a YAC that is able to propagate, segregate and be selected

22 Increasing the number of origins on a YAC suppresses GCR formation in our dpb11 mutant but enh

23 y in embryogenesis but can be rescued with a YAC containing the human beta-globin locus.

24 o identify gene sequences contained within a YAC by using cDNA representational difference analysis (

25 ansferred into mouse A9 cells to generate A9/YAC hybrids.

26 YAC DNA present in A9/YAC hybrids was further transferred by microcell fusion

27 pacing of approximately 300 kb and affording YAC coverage of approximately 92% of the mouse genome.

28 d increased splenic NK cell activity against YAC-1targets.

29 In vitro, cytotoxicity against YAC-1 and secretion of interferon gamma by TRAIL-null NK

30 nor was any NK-like killing observed against YAC-1 cells.

31 yr-Cys-amide (YC), acetyl-Tyr-Ala-Cys-amide (YAC), acetyl-Tyr-Ala-Ala-Cys-amide (YAAC), and acetyl-Ty

32 ontig spanning this region was assembled and YAC clones retrofitted with a selectable marker (neo) we

33 quencing of the gap regions, in both BAC and YAC forms, allowed us to generate a complete sequence of

34 The combined PAC/BAC transcript map and YAC scaffold presented here clarifies previously conflic

35 cloning vectors such as BACs, P1s, PACs and YACs.

36 d clone isolation, although in principle any YAC system can be used.

37 A mutant human APP YAC transgene was transferred to three inbred mouse stra

38 t age-dependent A beta deposition in the APP YAC transgenic model is dramatically altered depending o

39 06 nM) and which reduced amyloid-beta in APP-YAC mice with an ED(50) of 1 mg/kg (po).

40 AR YAC CAG100 will serve as a valuable reagent for the prod

41 rating vectors to successfully produce an AR YAC construct carrying 100 CAG repeats.

42 SBMA by crossing a highly representative AR YAC transgenic mouse model with 100 glutamines (AR100) a

43 To assess YAC stability, the human DNA inserts were internally mar

44 The BRCA1 BAC/YAC DNAs were isolated from bacterial cells and were use

45 arkers used in construction of the human BAC/YAC physical map, including autosomal dominant nocturnal

46 table marker and transferred as circular BAC/YACs in E. coli cells.

47 beta-YAC transgenic mice carrying the -117 HPFH mutation and

48 obin locus yeast artificial chromosome (beta-YAC) and analyzed globin gene expression during developm

49 obin locus yeast artificial chromosome (beta-YAC) bone marrow cells.

50 obin locus yeast artificial chromosome (beta-YAC) lines were produced in which the epsilon-globin gen

51 obin locus yeast artificial chromosome (beta-YAC) mice, showing that the hereditary persistence of fe

52 obin locus yeast artificial chromosome (beta-YAC) results in profound effects on globin gene expressi

53 obin locus yeast artificial chromosome (beta-YAC) transgenic mice, but deletion of the 352-bp region

54 recipitation of fetal liver tissue from beta-YAC transgenic mice demonstrated that GATA-1, FOG-1, and

55 e activity of beta-globin genes in GT6m beta-YAC transgenic mice.

56 pressed in postconception day E18 human beta-YAC transgenic mouse fetal liver.

57 n gene expression during development in beta-YAC transgenic mice.

58 relieves gamma-globin gene silencing in beta-YAC transgenic murine chemical inducer of dimerization h

59 In murine beta-YAC BMCs the vector mediated the activation of the silen

60 /MAR transfected a)K562 cells; b)murine beta-YAC bone marrow cells (BMC); c)human haematopoietic prog

61 e upstream region that is absent in the beta-YAC construct might be involved in gamma-gene reactivati

62 When WT beta-YAC bone marrow cells are treated with the OGA inhibitor

63 r localized to the region of overlap between YACs 911D5 and 844E3.

64 Ordered deletions of both YACs show that they define a 1-megabase pair contig span

65 multiple repeats, most of the primary BRCA1 YAC isolates did not contain detectable deletions and co

66 ther circular YACs, approximately 90kb BRCA1 YACs were efficiently and accurately retrofitted into ba

67 analysis of transfectants showed that BRCA1 YACs isolated by a TAR cloning contained a functional ge

68 It was mapped to YAC contig WC13.2 by YAC pool screening with an unambiguous hit to WI-4874, w

69 close enough to begin positional cloning by YAC walks.

70 Candidate YACs, identified by the markers located in the vicinity

71 We now show that in each of these cases, YAC clones found to contain a deletion breakpoint also c

72 e isolated and mapped a single non-chimaeric YAC (17IA12, 260-280 kb) containing D6S193 and D6S297.

73 devised a new yeast artificial chromomosme (YAC)-based assay for chromosome breakage to analyze frag

74 n yeast and bacterial artificial chromosome (YAC and BAC, respectively) maps.

75 tion of ATIC to yeast artificial chromosome (YAC) 914E7 previously reported to span the 2q35 break in

76 Both yeast artificial chromosome (YAC) and bacterial clone-based [bacterial artificial chr

77 ded to obtain a yeast artificial chromosome (YAC) carrying the AR gene and introduce CAG repeat mutat

78 y that a 320 kb yeast artificial chromosome (YAC) carrying the intact human CFTR gene can complement

79 1, containing a yeast artificial chromosome (YAC) cassette (a yeast selectable marker and a centromer

80 A 420 kb yeast artificial chromosome (YAC) clone, extending well beyond the most distant patie

81 osome (BAC) and yeast artificial chromosome (YAC) clones.

82 2)R gene from a yeast artificial chromosome (YAC) construct.

83 ration of human yeast artificial chromosome (YAC) constructs encompassing the MJD1 locus into which e

84 generation of a yeast artificial chromosome (YAC) containing the gene of interest.

85 ion of a 380 kb yeast artificial chromosome (YAC) containing the human PDGFRalpha gene.

86 nstruction of a yeast artificial chromosome (YAC) contig across this region.

87 e we describe a yeast artificial chromosome (YAC) contig covering the distal part of the mouse X chro

88 ll carcinoma, a yeast artificial chromosome (YAC) contig spanning the entire 3q26-27 region was const

89 We have aligned yeast artificial chromosome (YAC) inserts in a contig spanning the interval between t

90 a large-insert yeast artificial chromosome (YAC) library and then integrating the STS-content inform

91 1 Mb fragmented yeast artificial chromosome (YAC) mapping to this region completely suppresses the tu

92 hat carry large yeast artificial chromosome (YAC) or ligated cosmid constructs.

93 d them within a yeast artificial chromosome (YAC) scaffold by observing the retention patterns of sha

94 n from a 310 kb yeast artificial chromosome (YAC) that contains the entire CFTR gene with expression

95 ted from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among seve

96 that a 271-kbp yeast artificial chromosome (YAC) transgene could fully complement the loss of Gata2

97 mice carrying a yeast-artificial chromosome (YAC) transgene that over-expresses normal human FMRP (KO

98 ile X syndrome, yeast artificial chromosome (YAC) transgenic mice were generated in order to determin

99 a genomic-based yeast artificial chromosome (YAC) transgenic mouse model of AD.

100 Here, we used a yeast artificial chromosome (YAC) transgenic mouse model of HD to investigate the con

101 In a yeast artificial chromosome (YAC) we have engineered an approximately 170 kb region l

102 mice carrying a yeast artificial chromosome (YAC) with the intact human gene CFTR.

103 a 662-kb Gata3 yeast artificial chromosome (YAC), and these animals (termed G3YR mice) survived to a

104 lity of using a yeast artificial chromosome (YAC)-based reporter gene construct to define these eleme

105 s as a circular yeast artificial chromosome (YAC).

106 an ADE2-marked yeast artificial chromosome (YAC).

107 ative yeast/bacterial artificial chromosome (YAC/BAC) library covering the candidate region using a t

108 tification of a yeast artificial chromosome, YAC 927G4, that spans a translocation/duplication breakp

109 ecules such as yeast artificial chromosomes (YACs) has an advantage over smaller constructs in that a

110 Rs, exploiting yeast artificial chromosomes (YACs) in Saccharomyces cerevisiae.

111 ning different yeast artificial chromosomes (YACs) integrated into the same location in the genome pr

112 4-based linear yeast artificial chromosomes (YACs) into circular chromosomes that can also be propaga

113 ng a series of yeast artificial chromosomes (YACs) ordered in the CDR, and in five of eight cell line

114 Circular yeast artificial chromosomes (YACs) provide significant advantages for cloning and man

115 apped to three yeast artificial chromosomes (YACs) that contain human DNA from chromosome 6q26-27.

116 ystem based on yeast artificial chromosomes (YACs) was established for HCMV.

117 ibed to modify yeast artificial chromosomes (YACs) with cassettes that can be easily excised for embr

118 es (PACs) into yeast artificial chromosomes (YACs).

119 allows rescue of the fragment as a circular YAC/BAC molecule.

120 described system for generation of circular YAC derivatives can facilitate sequencing as well as fun

121 YAC inserts as a set of overlapping circular YAC/BACs, based on the use of an Alu-containing targetin

122 -selection integration system, with circular YAC lipofection technology to achieve single copy target

123 o exploit these advantages, because circular YACs are difficult to isolate and purify.

124 ately 2% of transformants contained circular YACs with the Tg.AC transgene sequences.

125 a method for purification of large circular YACs that is more reliable compared with previously desc

126 ped for the directed integration of circular YACs into mouse ES cells.

127 Similar to other circular YACs, approximately 90kb BRCA1 YACs were efficiently and

128 The circularized YACs were then targeted to the lox sites of the LES cell

129 A plasmid containing YAC vector sequences and a complementary '-neo-lox' cass

130 e showed that transcription from the deleted YAC was reduced by approximately 60%.

131 A robust, detailed YAC contig map is thus an important tool.

132 (CHO-K1) cell clones were derived using each YAC to assess the role that luc copy number and the pres

133 We show that this oriP-EBNA1-YAC can be stably maintained as unrearranged episomes in

134 s and enzymatic digestion of matrix-embedded YAC DNA to produce a solution that can be injected.

135 and (iii) a conditional centromere, enabling YAC DNA to be amplified in culture in the presence of ga

136 CG-repeats were identified within the entire YAC contig (not including FRA11B ), six of which map to

137 For example, YACs carrying full-size genes can be purified from yeast

138 t cancer, was not identified in the existing YAC and BAC libraries.

139 A contig of five YACs, nine BACs, and three P1s was constructed across th

140 FMR1 YAC transgenic mice overexpressing the human protein did

141 tromelia virus had elevated cytotoxicity for YAC-1 tumor cell targets compared with control animals.

142 o the candidate region was assembled by four YAC/BAC clones.

143 Smaller fragmented YACs give partial but not complete suppression.

144 rom our GAA repeat expansion-containing FRDA YAC transgenic mice reveal comparable epigenetic changes

145 n, we have generated two lines of human FRDA YAC transgenic mice that contain GAA repeat expansions w

146 ever, there is a difference between our FRDA YAC transgenic mice and other trinucleotide-repeat mouse

147 Sequence comparison of STSs amplified from YAC clones uniquely mapped to BP2 or BP3 showed two diff

148 n unique cDNA fragments were identified from YAC B30H3, which spans 330 kb in the human major histoco

149 the establishment of two lines of human FXN YAC transgenic mice that contain unstable GAA repeat exp

150 by crossbreeding of both lines of human FXN YAC transgenic mice with heterozygous Fxn knockout mice.

151 3-directed lacZ expression of a 625-kb Gata3 YAC transgene in mice mimics endogenous Gata3 expression

152 We found that the 662-kb Gata3 YAC transgene recapitulated Gata3 expression in the rena

153 have generated an advanced second-generation YAC contig map of the mouse genome that doubles both the

154 tional inactivity within a human beta-globin YAC expressed in transgenic mice.

155 milar to what has been obtained using globin YACs or ligated cosmids, we conclude that (1) globin tra

156 A 260-kb half-YAC clone derived from human chromosome 1q was mapped at

157 ss the 260-kb region encompassed by the half-YAC revealed the presence of EST sequence matches corres

158 R1) element of the UL54 promoter of the HCMV YAC.

159 This HCMV YAC backbone is defective for viral growth and lacks the

160 In the transgenesis experiments, HCMV YACs and derivatives generated in yeast were introduced

161 level of gene expression, we generated HCMV YACs containing a luciferase reporter gene inserted down

162 urred in the globin-genes of the adult HPFH2/YAC transgenic mice.

163 The difference in the phenotype of the HPFH2/YAC transgenic mice and the humans with HPFH2 mutation s

164 xpressed in the HPFH 2/beta locus YAC (HPFH2/YAC) transgenic mice in the early stage of development,

165 g to a premutation-sized allele into a human YAC carrying the FMR1 locus.

166 A series of mouse BAC and human YAC transgenes covering different intervals of the 450-k

167 This panel of human YAC transgenics, propagating a 1-Mb interval of chromoso

168 ration of an integrated somatic cell hybrid, YAC, and bacterial artificial chromosome contig spanning

169 alyses of rodent-human somatic-cell hybrids, YAC contigs, and FISH of normal or rearranged chromosome

170 short intervals corresponding to individual YAC clones.

171 n NK cell cytotoxicity to eliminate injected YAC-1 cells from the lungs.

172 ox-recombinants contain a full-length intact YAC.

173 This protocol details how to prepare intact YAC DNA for transgenesis of mice and involves separation

174 ed sequence-based markers from an integrated YAC STS-content/somatic cell hybrid breakpoint physical

175 , and detailed examination of the integrated YAC transgenes allowed the general localization of a num

176 The most efficient first step is isolating YAC (Yeast Artificial Chromosome) clones.

177 We found that a 120-kb YAC transgene, including 35 kb of 5' as well as 60 kb of

178 We have previously shown that a 130 kb YAC transgene contains multiple tissue-specific enhancer

179 A 178-kb YAC, containing a subgenomic fragment of HCMV encompassi

180 We now show that a 310 kb YAC clone, terminating just 5' of the breakpoint, fails

181 An approximately 350-kb YAC (y5'luc) was constructed by replacing CFTR with a lu

182 We have identified a 360 kb YAC that carries a cell senescence gene, SEN16.

183 In this 420 kb YAC a tauGFP-IRES-Neomycin reporter cassette has been in

184 iency was demonstrated by screening a 425 kb YAC known to contain the genes of four secretory or memb

185 line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsi

186 Introduction of a 60-kb YAC/BAC clone resulted in significant suppression of the

187 initive kidney development, and that a large YAC transgene faithfully recapitulated GATA-3 expression

188 ning the human tau gene into a stable linear YAC.

189 cassette was used to circularize two linear YACs containing genomic DNA from human chromosome 21.

190 gene was expressed in the HPFH 2/beta locus YAC (HPFH2/YAC) transgenic mice in the early stage of de

191 much stricter basis, we produced beta locus YAC transgenic mice carrying an exact beta locus replica

192 vely expressed granzyme A and directly lysed YAC-1 thymoma cells through granule exocytosis.

193 nd physical maps, human transcript gene map, YAC and PAC/BAC clone coverage, disease gene phenotype,

194 The combination of the BAC transcript map, YAC-to-BAC scaffold, and reference Human Genome Project

195 y (YTT) uses the WIBR/MIT-820 C57BL/6-mapped YAC library derived from the C57BL/6 mouse as the starti

196 sed CCG-trinucleotide repeats within a 40 Mb YAC contig spanning distal chromosome 11q.

197 We have constructed a mega-YAC/ STS map of this region that includes 436 YACs ancho

198 Moreover, YACs containing a mutant IR1 in the UL54 promoter led to

199 Four large-insert mouse YAC libraries from three different strains are included

200 ved from an internal promoter in a multicopy YAC transgenic line results in a microphthalmia phenotyp

201 By crossing PAX77 mice with a new YAC transgenic line that reports Pax6 expression (DTy54)

202 tained within three overlapping, nonchimeric YAC clones using sequential fluorescent in situ hybridiz

203 Here we utilize a novel YAC-based Foxa3Cre transgene to delete the winged helix

204 ured NK cytotoxicity in vivo by clearance of YAC-1 tumor cells from the lungs and by rejection of inc

205 The physical map is composed of YAC, BAC, PAC, and cosmid resources and spans a physical

206 en D1S491 and D1S205, and a 4.4-Mb contig of YAC clones of this region was constructed.

207 row transplants and in vitro by cytolysis of YAC-1 and Jurkat cells.

208 tem for comparing the bio-logical effects of YAC transgenes without the confounding influences of int

209 somes comprising 220 kb at the distal end of YAC 927G4, which we have used as hybridization probes un

210 In comparison with HCMV, infection of YAC transgenic NIH 3T3 lines with murine cytomegalovirus

211 We found that infection of YAC, but not plasmid, transgenic lines with HCMV was suf

212 GM-CSF, and TNF-alpha and in the killing of YAC-1 target cells.

213 ived MIF inhibited NK cell-mediated lysis of YAC-1 and uveal melanoma cells.

214 ition, 2B4L constitutively inhibits lysis of YAC-1 tumor targets.

215 also constructed a complete physical map of YAC and BAC clones covering the Ltxs1 region.

216 nalysis in Case 1 confirmed rearrangement of YAC 914E7 and fusion to ALK.

217 r YAC counterparts, and (2) the retention of YAC vector sequences in a transgene probably has no sign

218 nsgenesis of mice and involves separation of YAC DNA from yeast chromosomal DNA by pulsed field gel e

219 This system will make the study of YAC transgenic mice more reliable and reproducible, allo

220 A contig of YACs and BACs covering the nonrecombinant genomic region

221 Detailed examination of YACs containing human FBN1 reveal that the gene is 200 k

222 iched plasmid libraries or from subclones of YACs previously mapped to chromosome 6.

223 e 10q23-q24 and narrowed the interval to one YAC clone of 1410 kb.

224 ene characteristic of the plasmid, cosmid or YAC constructs used for production of transgenic mice.

225 ess likely to rearrange than their cosmid or YAC counterparts, and (2) the retention of YAC vector se

226 By interrogating a library of ordered YAC clones, we provide evidence for a chromosomal copy o

227 romeres using simultaneous FISH with ordered YAC probes and immunofluorescence with antibodies to CEN

228 YAC clones can be remapped and the original YAC skim strategy followed.

229 uction of correctly retrofitted, linear oriP YACs into human 293-EBNA cells by lipofection resulted i

230 In contrast, other YAC/BAC clones in the contig had neither metastasis nor

231 Finally, we used our YAC assay to assess the interplay of trans and cis facto

232 Two overlapping YAC clones showed greatly reduced colony-forming efficie

233 Six overlapping YACs spanning the chromosomal region of senescence activ

234 Etude du Polymorphisme Humain (CEPH) (Paris) YAC library with a degenerate alpha satellite probe.

235 We studied the developing cortex of PAX77 YAC transgenic mice carrying several copies of the human

236 Typically, TAR cloning produces positive YAC recombinants at a frequency of approximately 0.5%; t

237 This method produces positive YAC recombinants at a frequency of approximately 40%.

238 No transgene-positive YAC clones were detected when an ARS element was incorpo

239 In addition to the primarily YAC-based map, we located 1942 BAC (Bacterial Artificial

240 The purified YAC DNA is suitable for restriction enzyme digestion, DN

241 This method has been used to purify YACs up to 600 kb in size.

242 This method for isolating high-quality YAC DNA in microgram quantities should be valuable for f

243 on of an approximately 1.7-Mb sequence-ready YAC/BAC clone contig of 8p22-p23.

244 ene-specific targeting hook, the recombinant YAC product carries two copies of the loop sequence, the

245 The rescuing YAC did not display appropriate urogenital expression of

246 egion, we then constructed a high-resolution YAC/BAC/STS/EST physical map based on experimental and d

247 The resulting YAC can be used for generating transgenic animals and st

248 The resulting YAC is stably maintained in yeast and can be further mod

249 Retrofitted YACs were first transferred into mouse A9 cells to gener

250 tire CFTR gene with expression from the same YAC from which the DHS element had been deleted.

251 We found that feeding SCA3-YAC-84Q transgenic mice with dantrolene, a clinically re

252 A second YAC (y5'lucI) was similarly constructed but included a p

253 se they could kill neither NK cell-sensitive YAC-1 nor NK cell-resistant P815 and EL4 cells.

254 significantly enhanced against NK-sensitive YAC-1 cells and CL8-1 cells by rIL-18 administration to

255 e by lysis of chromium-labeled, NK-sensitive YAC-1 target cells.

256 We first generated seven YAC transgenic lines bearing a deletion of the 375-bp co

257 elected based on its size from among several YACs identified by screening a randomly chosen subset of

258 Within a single YAC, we have determined the order cen-HPAK3(5'-3')-DCX(3

259 and all three breakpoints mapped to a single YAC.

260 Re-isolation of megabase-size YAC inserts as a set of overlapping circular YAC/BACs, b

261 the Ods transgenic insertion or the Wt1-Sox9 YAC transgene overexpress the testis differentiation gen

262 We examined the replication of a stable YAC containing a 240-kb insert of DNA from the human T-c

263 Finally, these studies show that YAC transgenesis can be a useful tool in functional anal

264 The YAC also contains all the crucial elements responsible f

265 The YAC has been further engineered to insert LoxP sites fla

266 The YAC is maintained in an agarose gel matrix to avoid dama

267 The YAC transgene was expressed in neural crest cells, rescu

268 The YAC was modified by a strategy using homologous recombin

269 The YAC-based physical map directly facilitates positional c

270 ington's disease (HD), the R6/2 line and the YAC 128 mouse.

271 A translocation between the YAC and a yeast chromosome, whose breakpoint falls withi

272 ism was absent in knockout mice carrying the YAC transgene indicating functional rescue by the human

273 ectly isolate transcripts expressed from the YAC in mammalian cells.

274 However, the YAC transgene was expressed faithfully in oligodendrocyt

275 With the HAPPY Map the YAC clones can be remapped and the original YAC skim str

276 We found that most of the YAC expression sites and tissues are directly reflective

277 ng full-length and truncated versions of the YAC indicates the location and putative function of seve

278 but discarding most of the sequences of the YAC telomeric arms.

279 In the absence of Rad9 the YAC becomes unstable, undergoing deletions within the or

280 or, pLys2-neo, was developed to retrofit the YAC with the yeast lys2 gene, a selectable marker replac

281 In addition, we found that the YAC transgene did not prolong survival of Patch mutant m

282 We observed that the YAC transgene supported production of the human protein

283 We have mapped six new markers to the YAC; three of them are ESTs (WI-15078, WI-8751, and TCP1

284 When the YAC carries 10 TG's and 7 T's at the splice acceptor, th

285 The correct function of the LCR when the YAC is first transferred into the L-cell environment rai

286 d into yeast, the vector recombines with the YAC target sequences to form a circular molecule, retain

287 These YAC clones containing the apex of amplification were use

288 Gata2 hematopoietic function but that these YAC-rescued Gata2 null mutant mice die perinatally due t

289 repeats, have now been generated using these YAC constructs.

290 This YAC-based reporter system will provide a unique strategy

291 We have now modified this YAC to a circular molecule carrying both oriP and the EB

292 Replication of this YAC extends the length of S phase and causes cells to en

293 artificial chromosomes (PACs) spanning this YAC clone, and two PACs produced 'split' signals suggest

294 ation of such elements is pinpointed through YAC transgenic studies.

295 It was mapped to YAC contig WC13.2 by YAC pool screening with an unambigu

296 on of human telomeres and the oriP domain to YACs.

297 A translocation YAC was created that contains the breakpoint cluster reg

298 mammary tumor cell lines, while an unrelated YAC from chromosome 6q had no senescence activity.

299 we first performed FISH on ASPS cases using YAC probes for OATL1 (Xp11.23) and OATL2 (Xp11.21), and

300 e were highly responsive to stimulation with YAC-1 cells such that CD27(-)CD11b(+) NK cells from CR m