ライフサイエンスコーパス: acetyl phosphate

1 n increased capacity to be phosphorylated by acetyl phosphate.

2 heY in the presence of the CheY phosphodonor acetyl phosphate.

3 ncreased significantly after incubation with acetyl phosphate.

4 , high-energy phosphodonor molecules such as acetyl phosphate.

5 ho-DrrA exhibits much greater stability than acetyl phosphate.

6 a nucleophilic thiolate anion which attacks acetyl phosphate.

7 variant resembled the pH stability curve of acetyl phosphate.

8 It was not phosphorylated by acetyl phosphate.

9 d leads to activation by a process requiring acetyl phosphate.

10 (2+)-dependent autophosphorylation with [32P]acetyl phosphate.

11 Z-pta/ack strain, which could not synthesize acetyl phosphate.

12 estored by deleting the enzyme that degrades acetyl phosphate.

13 is phosphorylated by the small phosphodonor acetyl phosphate.

14 ation through either the Rcs phosphorelay or acetyl phosphate.

15 nts reacted faster with phosphoramidate than acetyl phosphate.

16 olites can function in a capacity similar to acetyl phosphate.

17 involved in the maintenance of intracellular acetyl phosphate.

18 group from ATP to acetate, yielding ADP and acetyl phosphate.

19 ration of any other phosphoryl donor such as acetyl phosphate.

20 olate anion of CoA on the carbonyl carbon of acetyl phosphate.

21 ated 10-fold by phosphorylation of CovR with acetyl phosphate.

22 VicR-P, which was prepared by reaction with acetyl phosphate.

23 ficity for longer-chain carboxylic acids and acetyl phosphate.

24 scillation through the signalling metabolite acetyl phosphate.

25 occurs by a mechanism that is independent of acetyl phosphate.

26 the addition of the small phosphoryl donor, acetyl phosphate.

27 m MprB or from small phosphodonors including acetyl phosphate.

28 t with the low-molecular-weight phosphodonor acetyl phosphate.

29 mes were incubated with ADP, Mg(II) ions and acetyl-phosphate.

30 A and pta, the two genes required to produce acetyl-phosphate.

31 roth cultures and precedes the production of acetyl phosphate, a high-energy molecule involved in int

32 ts support the long-standing hypothesis that acetyl phosphate, a physiologically relevant small molec

33 SpxB mutants synthesized 85% less acetyl-phosphate, a potential source of ATP.

34 Furthermore, when c-di-GMP levels are low, acetyl phosphate (Ac~P) is required for significant VpsR

35 product, glyoxylate, and increased levels of acetyl phosphate, acetoacetyl coenzyme A (acetoacetyl-Co

36 As part of our attempt to map the impact of acetyl phosphate (acetyl approximately P) on the entire

37 hy to measure the relative concentrations of acetyl phosphate, acetyl coenzyme A, ATP, and GTP over t

38 ays, and biochemical analysis, we identified acetyl phosphate (AcP) and carbamoyl phosphate (CP) as n

39 Acetyl phosphate (AcP) is a small-molecule metabolite th

40 to insulate themselves from cross-talk with acetyl phosphate (AcP) or other small phosphodonor metab

41 t respond to the status of the intracellular acetyl phosphate (acP) pool.

42 another universally conserved intermediate, acetyl phosphate (AcP), which bridges between thioester

43 requires phosphorylated RcsB and showed that acetyl-phosphate (AcP) is a phosphoryl group donor to Rc

44 was observed during planktonic growth, with acetyl phosphate acting as a phosphodonor to LytR.

45 ponse regulators, it remains unclear whether acetyl phosphate acts as a direct phospho donor or funct

46 reaction (acetate + ATP [Formula: see text] acetyl phosphate + ADP), with the exception of the Entam

47 nt, lacking the ability to generate ATP from acetyl phosphate, also failed to grow in xylose minimal

48 ating that both the levels of CsrABb and the acetyl phosphate and acetyl-CoA balance contribute to th

49 y low molecular weight phosphodonors such as acetyl phosphate and carbamoyl phosphate.

50 Acetyl phosphate and diphosphoimidazole were unaffected

51 d phosphorylated similarly to wild type with acetyl phosphate and faster (4-14x) with phosphoramidate

52 NodW can be phosphorylated in vitro by both acetyl phosphate and its cognate kinase, NodV.

53 This review will first introduce acetyl phosphate and its pathway.

54 the other Pi-independent pathway responds to acetyl phosphate and leads to activation by a process re

55 not become phosphorylated in the presence of acetyl phosphate and Mg(2+), although it appeared to bin

56 Elevated levels of acetyl phosphate and nlpE gene product can result in act

57 We propose that Arg310 binds acetyl phosphate and orients it for optimal nucleophilic

58 ated by small molecule phosphodonors such as acetyl phosphate and phosphoramidate, but it is phosphor

59 at of the previously described phosphodonors acetyl phosphate and phosphoramidate.

60 ext, it will describe emerging evidence that acetyl phosphate and/or its pathway can influence divers

61 ways involving the conversion of pyruvate to acetyl-phosphate and subsequently leading to fatty acid

62 opose that the in vivo activation of PhoB by acetyl phosphate (and perhaps other two-component respon

63 These are EutD (acetyl-CoA to acetyl phosphate) and EutG (acetaldehyde to ethanol).

64 ssB form a stable complex in the presence of acetyl phosphate, and together they form a ternary compl

65 mpaired in its ability to use either BvgS or acetyl phosphate as a substrate for phosphorylation.

66 by acetylation using the central metabolite acetyl phosphate as the acetyl donor.

67 ant to wild-type OmpR, are phosphorylated by acetyl phosphate as well as the cognate kinase EnvZ, and

68 was prevented by preincubation with acetate, acetyl phosphate, ATP, or ADP, suggesting that E385 is l

69 eral small-molecule phosphodonors, including acetyl phosphate, benzoyl phosphate, carbamoyl phosphate

70 atory system response regulator activated by acetyl phosphate; BosR, an unorthodox DNA-binding protei

71 ontaining glucose, CpxR is phosphorylated by acetyl phosphate but cannot be dephosphorylated, resulti

72 phosphorylation by a small phosphate donor, acetyl phosphate, but not phosphorylation by the kinase

73 reversible magnesium-dependent synthesis of acetyl phosphate by transfer of the ATP gamma-phosphoryl

74 Here we show that activation of PhoB by acetyl phosphate can require the sensor kinase EnvZ.

75 Although acetyl phosphate clearly signals through two-component r

76 The classic sensor kinase EnvZ and acetyl phosphate collaborate to produce the optimum leve

77 ough sensing of an intracellular metabolite, acetyl phosphate, controls the expression of two key enz

78 results also demonstrated that acetyl-CoA or acetyl-phosphate could acetylate MDH chemically in vitro

79 We propose that acetyl phosphate-dependent acetylation is a response to

80 t lysines depends largely on a non-enzymatic acetyl phosphate-dependent mechanism.

81 plasmic stress-responsive promoter cpxP: (i) acetyl phosphate-dependent phosphorylation of the respon

82 or mutations eliminating the ability to make acetyl phosphate did not alter the high level of degP ex

83 mutant, which should not be able synthesize acetyl phosphate, failed to express the van genes, where

84 EutQ and EutP also synthesized acetyl-phosphate from ATP and acetate.

85 d exogenous addition of phospho-donors (e.g. acetyl phosphate) have little to no effect on phosphoryl

86 Purified UhpA was phosphorylated by acetyl phosphate in a reaction that was dependent on Mg2

87 It can accept phosphate from acetyl phosphate in a reaction typical of RRs, with tran

88 nts were screened to investigate the role of acetyl phosphate in UTI pathogenesis.

89 ated that the intracellular concentration of acetyl phosphate in wild-type cells reaches at least 3 m

90 ions that decrease the pool of intracellular acetyl-phosphate in E. coli, we found a reduction in the

91 nvasion gene expression by the production of acetyl-phosphate in the bacterial cytoplasm, a pathway t

92 r fructose-6-phosphate) to acetate precursor acetyl phosphate, in an engineered strain of the model c

93 kinase VanS, the noncognate kinase PhoR, or acetyl phosphate, indicating that phospho-VanR (P-VanR)

94 nterferes with activation of VanR by PhoR or acetyl phosphate, indicating that VanS also acts as a P-

95 phate acyltransferase to convert formate and acetyl-phosphate into the central biosynthetic intermedi

96 Since acetyl phosphate is a central metabolite, this ability w

97 etic analyses also indicated that binding of acetyl phosphate is more dependent on interactions of th

98 of RpoS and OspC expression, suggesting that acetyl-phosphate is an activator of Rrp2.

99 demonstrate that the intermediary metabolite acetyl-phosphate is an important acetyl donor that contr

100 catalyzes the interconversion of acetate and acetyl phosphate, is nearly ubiquitous in bacteria but i

101 Additionally, EnvZ, but not acetyl phosphate, is required for maximal expression of

102 ented with acetate which presumably restores acetyl phosphate levels by the action of acetate kinase,

103 nscription of ompF under conditions in which acetyl phosphate levels were high.

104 Affinity probing of IL-1ra with methyl acetyl phosphate (MAP) in combination with proteolytic d

105 TopA is decreased by in vitro non-enzymatic acetyl phosphate mediated lysine acetylation, and the pr

106 rified PhoP* protein autophosphorylated from acetyl phosphate more efficiently than the wild-type Pho

107 Neither acetyl phosphate nor ATP affected the binding ability, a

108 nt metabolic pathways, which proceed through acetyl phosphate or acetyl-AMP intermediates.

109 h either of the small-molecule phosphodonors acetyl phosphate or carbamoyl phosphate under conditions

110 ence was obtained to support models in which acetyl phosphate or the PTS system contributes to MviA p

111 d with a small phosphorylating agent such as acetyl phosphate or with protein-catalyzed phosphorylati

112 concentration of acetate metabolites such as acetyl-phosphate or acetyladenylate.

113 kphos/KS) for reaction with phosphoramidate, acetyl phosphate, or monophosphoimidazole, with the grea

114 result of requiring a proper cycling of the acetyl phosphate pathway for colonization of the upper u

115 a mutant with a metabolic disruption to the acetyl-phosphate pathway.

116 We demonstrate that acetyl phosphate phosphorylates OmpR at aspartate 55, th

117 s suggest that in the absence of vancomycin, acetyl phosphate phosphorylates VanR, and VanS acts as a

118 yrophosphate (PPi)/inorganic phosphate (Pi) (acetyl phosphate + Pi [Formula: see text] acetate + PPi)

119 te oxidases which decarboxylates pyruvate to acetyl phosphate plus H2O2 and CO2.

120 e decarboxylase, pyruvate decarboxylase, the acetyl phosphate-producing pyruvate oxidase, and the ace

121 The substrates ATP, ADP, acetate, and acetyl phosphate protected against inactivation suggesti

122 ntral metabolic pathways and overlapped with acetyl phosphate-regulated acetylation sites, we deleted

123 Here we show that phosphorylation by acetyl phosphate results in dimerization of CovR.

124 pothesis and list predicted properties of an acetyl phosphate-sensitive pathway.

125 The side chains may include O-acetyl, phosphate, sialic acid, and other moieties.

126 g a PhoP variant that is phosphorylated from acetyl phosphate, some of the mutants failed to repress

127 le only after incubation of the protein with acetyl phosphate, suggesting that phosphorylation is nec

128 m pta gene, encoding the enzyme critical for acetyl phosphate synthesis, is not significantly affecte

129 ction was also increased in a strain lacking acetyl-phosphate that can donate phosphate groups to Omp

130 to the accumulation of key metabolites, like acetyl phosphate, that facilitate the adaptation of B. b

131 A heterologous system consisting of acetyl phosphate, the bacterial chemotaxis response regu

132 Acetyl phosphate, the intermediate of the AckA-Pta pathw

133 It also has been proposed that acetyl phosphate, the intermediate of the phosphotransac

134 placed under the transcriptional control of acetyl phosphate, the system oscillates when the glycoly

135 e CpxR could receive a phosphoryl group from acetyl phosphate, this global signal was not the primary

136 ltransferase (Pta) involved in conversion of acetyl phosphate to acetyl-coenzyme A (acetyl-CoA) and p

137 or Al(3+) promotes phosphoryl transfer from acetyl phosphate to all canonical NDPs to produce their

138 reversible transfer of the acetyl group from acetyl phosphate to coenzyme A (CoA), forming acetyl-CoA

139 reversible transfer of the acetyl group from acetyl phosphate to coenzyme A (CoA), forming acetyl-CoA

140 reversible transfer of the acetyl group from acetyl phosphate to coenzyme A (CoA): CH(3)COOPO(3)(2-)

141 phate acetyl-transferase (Pta) that converts acetyl-phosphate to acetyl-coenzyme A (CoA), led to the

142 DNase I footprinting determined that the acetyl phosphate-treated CsrR was binding to key sequenc

143 Activation of adhI transcription by acetyl-phosphate-treated AfdR in vitro is inhibited by a

144 pyruvate oxidase, which converts pyruvate to acetyl-phosphate under non-CCR-inducing growth condition

145 Thus, acetyl phosphate was not involved in the regulation of o

146 that of the wild type, suggesting that more acetyl-phosphate was being converted to acetyl-CoA in th

147 f pta and ackA increases the accumulation of acetyl phosphate when SdhX is expressed.

148 This enzyme mediates the production of acetyl phosphate, which has been shown to serve as a low

149 Acetyl phosphate, which phosphorylates RssB, is required

150 ng aerobic growth, SpxB synthesizes H2O2 and acetyl phosphate, which play roles in metabolism, signal

151 al for degradation of the signaling molecule acetyl phosphate, while the downstream pta gene, encodin

152 rom small-molecule phosphate donors, such as acetyl phosphate, while the VirGI77V/D52E allele carries