ライフサイエンスコーパス: acid-fast

1 ishing characteristics: its ability to stain acid-fast and its ability to cause long-term latent infe

2 ogy and staining were conserved for modified acid-fast and modified trichrome stains.

3 al staining procedures, such as the modified acid-fast and safranin stains, are generally employed.

4 a, trichrome, chromotrope, Gram-chromotrope, acid-fast, and safranin stains.

5 oocysts retained full fluorescence, modified acid-fast- and safranin-stained smears of Cryptosporidiu

6 rescein diacetate, quantitative culture, and acid-fast auramine microscopy were all performed in trip

7 mycobacterial growth and those with positive acid fast bacilli (AFB) growth were tested to detect myc

8 Acid Fast Bacilli (AFB) microscopy smear remains the mos

9 phenotypic TB detection tests in the region [acid fast bacilli smear microscopy and Mycobacteria Grow

10 Higher LL-37 concentrations correlated with acid fast bacilli sputum smear positivity and weight gt

11 tions from silica-exposed mice had many more acid fast bacilli(+) (AFB(+)) organisms than from contro

12 h, and 94 (92%) had sputa smear-positive for acid fast bacilli.

13 ulated the percentage of lipid body-positive acid-fast bacilli (%LB + AFB) on sputum smears.

14 system using Middlebrook broth selective for acid-fast bacilli (13A medium).

15 ns submitted for microscopy for detection of acid-fast bacilli (AFB) and for mycobacterial culture an

16 Sputum smears for acid-fast bacilli (AFB) are the primary methods for diag

17 Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than

18 Detection of acid-fast bacilli (AFB) by sputum smear supports treatme

19 sputum corresponded well with the numbers of acid-fast bacilli (AFB) counted by microscopy.

20 rotizing and non-necrotizing granulomas, and acid-fast bacilli (AFB) culture from the surgically remo

21 Smears were positive for acid-fast bacilli (AFB) in 63% (677 of 1,082) of specime

22 Manual reading of fluorescent acid-fast bacilli (AFB) microscopy slides is time-intens

23 Xpert, Lowenstein-Jensen (L-J) culture, and acid-fast bacilli (AFB) microscopy.

24 pic examination of respiratory specimens for acid-fast bacilli (AFB) plays a key role in the initial

25 A total of 575 participants had acid-fast bacilli (AFB) positive Mycobacterial Growth In

26 red to 2 sputum samples, each evaluated with acid-fast bacilli (AFB) smear and mycobacterial culture

27 of tuberculosis (TB) are more accurate than acid-fast bacilli (AFB) smear microscopy and are faster

28 Sputum acid-fast bacilli (AFB) smear microscopy has suboptimal

29 y isolation pending results of serial sputum acid-fast bacilli (AFB) smear microscopy is standard pra

30 ies for identification of MTBC directly from acid-fast bacilli (AFB) smear-positive broth cultures, i

31 Grocott's methenamine silver (GMS) stain and acid-fast bacilli (AFB) stain of the tissue itself were

32 Investigations such as culture, acid-fast bacilli (AFB) staining, and histopathology hav

33 farct, adrenal necrosis, and hemorrhage, and acid-fast bacilli (AFB) were seen in the lung, liver, ki

34 ing collections; and quantitative smears for acid-fast bacilli (AFB) with quantitative cultures.

35 ogy, immunohistochemistry (IHC) staining for acid-fast bacilli (AFB), and mycobacterial polymerase ch

36 ure for M. ulcerans, or a smear positive for acid-fast bacilli (AFB), together with a possible histol

37 l of 106 specimens were culture positive for acid-fast bacilli (AFB).

38 esses digital microscopic images to identify acid-fast bacilli (AFB).

39 generally requires specialized cultures for acid-fast bacilli (AFB; AFB cultures).

40 60 and Middlebrook 7H11 biplates), smear for acid-fast bacilli (AFB; auramine O), and clinical course

41 imen inclusion were (i) a positive smear for acid-fast bacilli (n = 54) and (ii) the source if the sm

42 hu10Tg mice showed marked increases in both acid-fast bacilli and host macrophages.

43 agement of highly infectious agents, such as acid-fast bacilli and systemic fungi, were revealed.

44 44 nerves from armadillos were screened for acid-fast bacilli and thin sections were examined ultras

45 Bronchoscopic washings revealed acid-fast bacilli and were culture positive for M tuberc

46 ee sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Ne

47 nfirmed cases, and yield of sputum smear for acid-fast bacilli cases.

48 Only 46 (32%) of these 143 patients had acid-fast bacilli detected in smears; acid-fast bacilli

49 patients with smears that were positive for acid-fast bacilli had a median treatment interval of 3 d

50 l-defective bacteria which later reverted to acid-fast bacilli have been isolated from sarcoid tissue

51 s included smears of aspirated materials for acid-fast bacilli in 11, mycobacterial culture in 14, an

52 sed on passive case finding and detection of acid-fast bacilli in sputum samples to diagnose pulmonar

53 sure the progressive reduction of numbers of acid-fast bacilli in the sputum smear and the clearance

54 had a respirator-fit testing program but no acid-fast bacilli isolation rooms.

55 Cultures of valve tissue for acid-fast bacilli might be considered in some cases of a

56 rculosis but sputum smears were negative for acid-fast bacilli on 3 consecutive days) and 22,716 case

57 Of 60 samples, 15 showed acid-fast bacilli on special staining.

58 losis, it often manifests without detectable acid-fast bacilli on sputum microscopy.

59 ted using the MTBDRplus assay after positive acid-fast bacilli or culture.

60 d with broth from MGIT cultures positive for acid-fast bacilli or growth on a solid medium, we compar

61 burden, with smear grades scanty or 1+ (1-99 acid-fast bacilli per 100 fields).

62 ious tuberculosis by simple sputum smear for acid-fast bacilli remains an important tool, and more ra

63 attern, and increase in the highest grade of acid-fast bacilli smear (AFS).

64 erformance scores (P = 0.016), higher sputum acid-fast bacilli smear microscopy grades (P = 0.007), l

65 We prospectively analyzed 60 acid-fast bacilli smear-positive clinical sputum samples

66 Patients in whom acid-fast bacilli smear-positive pulmonary tuberculosis

67 Six hundred and fifty-seven direct patient acid-fast bacilli smear-positive specimens resistant to

68 dictor of infection than the standard sputum acid-fast bacilli smear.

69 thological testing of skin-biopsy specimens, acid-fast bacilli smears, and microbial cultures and ant

70 nosis, 69% of MDR-TB cases were positive for acid-fast bacilli sputum smears and 43% had cavitary dis

71 ne (3%) case of a lymph node with a positive acid-fast bacilli stain.

72 We modified microscopy for acid-fast bacilli to diagnose tuberculosis (TB) using sm

73 ucibacillary specimens that are negative for acid-fast bacilli using smear microscopy.

74 The sensitivity of mSDA for detecting acid-fast bacilli was 96.4% compared to that of MGIT cul

75 ts had acid-fast bacilli detected in smears; acid-fast bacilli were detected in the first submitted s

76 No acid-fast bacilli were seen, but small budding yeasts ch

77 red around and within the necrotic core, and acid-fast bacilli were visible both within macrophages a

78 and one necrotizing granuloma (negative for acid-fast bacilli) that grew Mycobacterium kansasii on c

79 bacteria were found to be classic rod-shaped acid-fast bacilli, while in the stationary phase M. smeg

80 f 596 blocks containing nerve, 36% contained acid-fast bacilli.

81 than H37Ra, based on the numbers of CFU and acid-fast bacilli.

82 nd were examined for granuloma formation and acid-fast bacilli.

83 ost cases were not sputum-smear positive for acid-fast bacilli.

84 on associated with areas of inflammation and acid-fast bacilli.

85 heterogeneous morphology and distribution of acid-fast bacilli; only at the surface of cavities, i.e.

86 Frequency of CSF acid-fast-bacilli smear positivity was 8.9% (95% CI 5.0-

87 is treatment, directly observed therapy, and acid-fast-bacilli smear-positivity to obtain adjusted od

88 Eighty percent (680/848) of patients having acid-fast-bacilli-smear-positive specimens had MTD perfo

89 cepacia selective agar (BCSA) and a standard acid-fast bacillus (AFB) culture method for the isolatio

90 which was likely overlooked by conventional acid-fast bacillus (AFB) culture methods.

91 Average time to acid-fast bacillus (AFB) detection and identification to

92 bioaerosols generated by the Xpert assay to acid-fast bacillus (AFB) microscope slide smear preparat

93 culture positive, of which 276 (72.8%) were acid-fast bacillus (AFB) smear positive.

94 successfully recovered NTM from samples with acid-fast bacillus (AFB) smear scores of 3+/4+ (i.e., 2

95 berculosis as measured by detection of rRNA, acid-fast bacillus (AFB) smear, and culture was determin

96 allenging subpopulation of IGRA-positive but acid-fast bacillus (AFB) smear-negative TB suspects.

97 MTB) was employed to detect organisms in 135 acid-fast bacillus (AFB) smear-positive respiratory spec

98 rize mutations in the gyrA and gyrB genes of acid-fast bacillus (AFB) smear-positive sediments or of

99 To evaluate the efficacy of three sputum acid-fast bacillus (AFB) smears to rule out pulmonary tu

100 ne precautions" category from three negative acid-fast bacillus (AFB) smears to two, or even one.

101 Acid-fast bacillus (AFB) spinal osteomyelitis in a patie

102 for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.

103 ypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining.

104 In acid-fast bacillus (AFB)-negative sputum, sensitivity wa

105 CR for use with respiratory, nonrespiratory, acid-fast bacillus (AFB)-positive and AFB-negative speci

106 identify Mycobacterium species directly from acid-fast bacillus (AFB)-positive mycobacterial culture

107 CSF did not grow any bacteria, fungus, or acid-fast bacillus at culture.

108 not significantly different from that of an acid-fast bacillus culture (AFC) which includes both MGI

109 CSF fungal culture, 267, $999, and 67 h; CSF acid-fast bacillus culture, 275, $1,662, and 124 h; stoo

110 thods was as follows: fluorochrome stain for acid-fast bacillus microscopy (47%); radiometric methods

111 nded techniques increased from 44 to 73% for acid-fast bacillus microscopy, from 27 to 37% for primar

112 The acid-fast bacillus Mycobacterium tuberculosis is often t

113 The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debri

114 uberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients

115 The 2002 external QA guidelines for acid-fast bacillus smear microscopy were implemented, an

116 30 strain were less likely to be respiratory acid-fast bacillus smear positive (51% versus 72%).

117 the model, the presence of cavitary lesions, acid-fast bacillus smear positivity, and multilobar pres

118 same performance values for the fluorochrome acid-fast bacillus smear were 33, 98, 62, and 94%, respe

119 The diagnostic yield of acid-fast bacillus smear with CB-18 in the absence of fl

120 ex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens.

121 uberculosis, revealed by positive results of acid-fast bacillus smears.

122 It is a curved, acid-fast bacillus that is naturally attenuated with a n

123 terium triplex was first named in 1996 as an acid-fast bacillus with features that most resemble Myco

124 zed: not performing fungal or mycobacterial (acid-fast bacillus) cultures on cerebrospinal fluid (CSF

125 ny evidence of growth of bacteria, fungi, or acid-fast bacillus.

126 tum specimens is very high and that only two acid-fast-bacillus smear-positive specimens are needed f

127 The yield of mycobacterial culture from acid-fast-bacillus smear-positive sputum specimens was 3

128 Sputum acid-fast-bacillus smears became negative in all patient

129 ycobacterium tuberculosis is the hallmark of acid fast bacteria and is responsible for much of its ph

130 cally relevant Gram-negative, -positive, and acid fast bacteria.

131 Fifty-two specimens were smear positive for acid-fast bacteria (AFB); M. tuberculosis was isolated f

132 J) systems for the recovery of mycobacteria (acid-fast bacteria [AFB]) from 1,441 clinical specimens.

133 Acid-fast bacteria and ESAT-6-expressing leukocytes were

134 Histopathology of the specimen revealed acid-fast bacteria and M. haemophilum was identified by

135 dered particularly in cases where smears for acid-fast bacteria are positive but cultures are negativ

136 tient was referred for examination; however, acid-fast bacteria test results were negative.

137 uberculous mycobacteria are a large group of acid-fast bacteria that are very widely distributed in t

138 ous mycobacteria (NTM) are a large family of acid-fast bacteria, widespread in the environment.

139 All the processed specimens which were acid-fast bacterium (AFB) smear positive were used for s

140 erformance of the MID-DRS was also tested on acid-fast-bacterium (AFB)-positive clinical specimens, r

141 Mesoscopy combined with the novel CUBIC Acid-Fast (CAF) staining procedure enables a quantitativ

142 ted for use in the routine identification of acid-fast isolates growing in BACTEC 12B and 13A liquid

143 PCR-RFLP identified 100 of 103 acid-fast isolates recovered from 610 patient specimens

144 Seventy-six acid-fast isolates were identified as M. paratuberculosi

145 mparison of UV fluorescence against modified acid-fast (MAF) stains using 50 (35 Cyclospora positive,

146 laboratories using fluorescence staining for acid-fast microscopy has increased from 71.4 to 85.7%, t

147 and human immunodeficiency virus infection, acid-fast microscopy is highly sensitive (93.1%) and spe

148 ratories in low-income countries report that acid-fast microscopy is insensitive and nonspecific.

149 Acid-fast microscopy results changed little during early

150 as 5.1% (IQR, 2.4%-11%) the concentration of acid-fast microscopy-positive bacteria (2069 [IQR, 1358-

151 erculosis using FDA microscopy, culture, and acid-fast microscopy.

152 ere compared with the results of culture and acid-fast microscopy.

153 Second, acid-fast MTB bacilli are difficult to lyse.

154 tablished experimental animal infections are acid-fast negative, clearly cell wall changes are occurr

155 spora requires either the modified Kinyoun's acid-fast or safranin stains, which are not part of the

156 n at room temperature, a very slowly growing acid-fast organism was isolated.

157 guous method for species assignment of these acid-fast organisms for diagnostic purposes.

158 ages with typical numerous PAS-positive, non-acid fast particles.

159 Mycobacterium tuberculosis is an acid-fast pathogen of humans and the etiological agent o

160 In comparison to spoligotyping of acid-fast-positive MGIT cultures, percent agreement betw

161 of Cyclospora stain variably by the modified acid-fast procedure, resulting in the possible misidenti

162 nd 1999 from five spontaneous disease cases, acid-fast rods were consistently found within lesions, a

163 Acid-fast rods were detected within the smallest lesions

164 l growth, which (if present) are examined by acid-fast smear analysis.

165 in only 1 patient who was positive by sputum acid-fast smear and spent substantial amounts of time at

166 ultaneously assessed the diagnostic yield of acid-fast smear in this same cohort.

167 These results indicate that acid-fast smear using >/= 5.0 ml of sputum increases sen

168 Sputum quantitative culture, acid-fast smear, days-to-positive by BACTEC, and Mycobac

169 sensitivity and specificity of the test with acid-fast smear, mycobacterial culture, and clinical eva

170 verall sensitivities of the assay, including acid-fast smear-positive and -negative specimens, were 9

171 Both acid-fast smear-positive and smear-negative respiratory

172 st was evaluated using a combined set of 338 acid-fast smear-positive and smear-negative, respiratory

173 s of pulmonary tuberculosis in patients with acid-fast smear-positive sputum samples since 1996.

174 ely evaluated 183 hospitalized patients with acid-fast smear-positive tuberculosis who gave no histor

175 Of 179 acid-fast, smear-positive specimens that were culture po

176 because culture takes weeks and conventional acid-fast sputum microscopy and molecular tests cannot d

177 ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques.

178 Modified Kinyoun's acid-fast stain is the most commonly used stain to ident

179 Without specific training, using the Kinyoun acid-fast stain, definitive cording was found in 237 of

180 pings using Gram stain, the modified Kinyoun acid-fast stain, or both.

181 Captured Mtb bacilli were predominantly acid-fast stain-negative and poorly culturable; however,

182 for Cryptosporidium, and modified Kinyoun's acid-fast stained smears for the detection of Cryptospor

183 (Alexon-Trend, Inc.), and modified Kinyoun's acid-fast stained smears were 68, 70, and 78%, respectiv

184 Kinyoun acid-fast stained smears were prepared from 666 positive

185 SpecT microplate assay or modified Kinyoun's acid-fast stained smears.

186 Microscopic examination of acid-fast-stained sputum smears is the current standard

187 made by concentration of stools followed by acid-fast staining (AF) or immunofluorescent staining.

188 10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (

189 Nocardia sp. due, in part, to its partially acid-fast staining characteristic, morphology, and odor.

190 In addition, cell morphology and acid-fast staining characteristics showed variations wit

191 respiratory specimens to improve culture and acid-fast staining of mycobacteria is introduced.

192 nents work together to lead to the classical acid-fast staining of mycobacteria.

193 cteristic rod shape and developed a punctate acid-fast staining pattern with carbolfuchsin.

194 t, and 96 h for H37Rv and 104 h for H37Ra by acid-fast staining).

195 Gram staining, acid-fast staining, and lactic acid, cryptococcal antige

196 CSF Gram staining, acid-fast staining, cryptococcal antigen, varicella-zost

197 Mycobacterial growth was evaluated by acid-fast staining, electron microscopy, and colony-form

198 taining procedure, besides being superior to acid-fast staining, is fast, reliable, and easy to perfo

199 n mycolic acid synthesis, results in loss of acid-fast staining.

200 ith trichrome, iron-hematoxylin, or modified acid-fast stains or the Meridian Bioscience, Inc., Giard

201 ng staining with both trichrome and modified acid-fast stains.

202 pifluorescence microscopy), (ii) in modified acid-fast-, trichrome-, and safranin-stained smears, and