ライフサイエンスコーパス: acini

1 disrupted ductal tree and a >90% deficit of acini.

2 ot IL12, after co-incubation with pancreatic acini.

3 d tubule formation and stunted the growth of acini.

4 and survival in the luminal space of mammary acini.

5 n of EPI64 and EPI64B in isolated pancreatic acini.

6 repress HNF6 and are expressed in developing acini.

7 astoma cells and in three-dimensional MCF10A acini.

8 ls formed enlarged and poorly differentiated acini.

9 ltons [SNAP23], and VAMP2) in rat pancreatic acini.

10 t cohesively as they divide to assemble into acini.

11 apical polarity longitudinally for the same acini.

12 ng remained quiescent within growth-arrested acini.

13 on CCK-8-stimulated exocytosis in pancreatic acini.

14 rchitecture of silicon rubber casts of mouse acini.

15 ow-derived macrophages after incubation with acini.

16 dent model of cell damage for salivary gland acini.

17 ease from lacrimal gland pieces but not from acini.

18 S results in lumen-filled mammary epithelial acini.

19 onents of this pathway in rat lacrimal gland acini.

20 s of acute pancreatitis and human pancreatic acini.

21 pieces or digested with collagenase to form acini.

22 ignant cell lines compared with nonmalignant acini.

23 as has been observed in Her2/Neu-expressing acini.

24 uced by the epithelial cells of the prostate acini.

25 hile no significant changes were observed in acini.

26 ne, develop from single cells into polarized acini.

27 iciently than cells harvested from uninduced acini.

28 not required for the formation of polarised acini.

29 tic peptides and fibroblast growth factor on acini.

30 GEF III, were identified in mouse pancreatic acini.

31 ranching ducts ending in hollow, sphere-like acini.

32 s and accelerated lumen formation in mammary acini.

33 structs of these proteins into permeabilized acini.

34 is significantly reduced Ad5 transduction of acini.

35 ned axonal projections enter each individual acini.

36 d ducts, supernumerary end buds, and ectopic acini.

37 hose previously observed in dispersed rodent acini.

38 tis-causing treatments as observed in rodent acini.

39 morphogenesis and homeostasis in epithelial acini.

40 to enlarged, disorganized, three-dimensional acini.

41 oked oscillatory Ca(2+) increases across the acini.

42 ymphatic vessels located close to and around acini.

43 rptive function of Na/K-ATPase in the type I acini.

44 n by suppressing fluid transport in type III acini.

45 ctures opening towards the lumen in type-III acini.

46 large, lobular structures rather than hollow acini.

47 body and extended to the apical parts of the acini.

48 etion and increased the number of functional acini.

49 hrough differentiation of the PanIN cells to acini, accompanied by re-expression of the acinar transc

50 es the amount of surfactant delivered to the acini after losing a portion to coating the involved air

51 The image-based segmentation of individual acini allowed the computation of acinar volume and surfa

52 111 reorganizes mammary cells into polarized acini, allowing both the exposure of the prolactin recep

53 etected in cells and in the apical region of acini along AQP5.

54 Human acini also secreted inflammatory mediators elevated in a

55 VAMP8(-/-) acini also showed a >90% decrease in the early endosomal

56 Epithelial cells spontaneously form acini (also known as cysts or spheroids) with a single,

57 examined in rats (1) in vitro with isolated acini and (2) in vivo with an acid challenge.

58 were fully mechanically isolated from other acini and also from the bulk gel by box-cuts with a side

59 analysis revealed restriction of CEACAM20 to acini and CEACAM1 to tubule structures, respectively.

60 lrECM prevents formation of organized breast acini and disrupts growth arrest.

61 found differentially expressed in the serous acini and duct cells of all major salivary glands.

62 d PKA activation in both isolated pancreatic acini and duct fragments.

63 xpressed protein was localized to pancreatic acini and ductal cells.

64 C6 and AC9 were expressed in both pancreatic acini and ducts, whereas AC7 was expressed only in pancr

65 eral AC isoforms are expressed in pancreatic acini and ducts.

66 could be localized to the stroma surrounding acini and ducts.

67 or both the formation of polarised secretory acini and for the transcription of tissue-specific milk

68 posed of 2 secretory compartments, including acini and granular ducts connected by intercalated ducts

69 he stalk region) identities that produce the acini and higher order ducts, respectively.

70 c central neurons innervating salivary gland acini and identify different neuropeptides and their pre

71 of D52 followed by Rab5 and EEA1 in isolated acini and in in vivo The loss of D52 occurred as a conse

72 ed by high cyclin E both in cultured mammary acini and in mammary epithelial tissues in a mouse model

73 herent rotation is present in normal mammary acini and kidney cells but absent in cancerous cells.

74 polarity are essential for the formation of acini and link the functional relevance of CAMo to the e

75 ar effects to those found in Dicer-deficient acini and metaplastic cells, namely induction of HNF6 an

76 PI3K/AKT signaling, is overexpressed in the acini and PDAC of Pdx1-Cre;Kras(G12D/+);p53(f/+) (PKC) m

77 Pancreatic acini and peripheral blood mononuclear cells were expose

78 Isolated pancreatic acini and spleen-derived leukocytes were used for ex viv

79 the disruption of architecture in epithelial acini and suggest that ERK1/2 can promote noninvasive mo

80 pressed in a subset of normal prostate gland acini and that Dkk-3 expression is reduced in prostate t

81 penton base, stimulated macropinocytosis in acini and that inhibition of macropinocytosis significan

82 l death responses in mouse and human primary acini and the acinar cell line AR42J.

83 cholinergic receptors in isolated pancreatic acini and the mechanisms responsible for other neurotran

84 of dynamic processes, e.g., the polarity of acini and the merging of polarized structures, upon tran

85 extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized prol

86 ls, becoming highly prominent in peritumoral acini, and particularly high in acinar ductal metaplasia

87 prived cells in the luminal space of mammary acini, and the discovery that antioxidants facilitate th

88 cytosis processes in cultured rat pancreatic acini (apical blockade, basolateral exocytosis, and fusi

89 d debris (50.9%), gland dropout (42.8%), and acini appearance (54.5%).

90 ical outcomes ranged from fair agreement for acini appearance (kappa(w) = 0.23, 95% CI = 0.14-0.32) a

91 ading in a real-time examination compared to acini appearance and lid debris.

92 tion of partial glands in the lower lid, and acini appearance by the presence/absence of grape-like c

93 raphy grading of meibomian gland atrophy and acini appearance, and slit-lamp grading of lid debris an

94 3 zeta overexpression severely disrupted the acini architecture resulting in luminal filling.

95 Pancreatic acini are completely devoid of zymogen granules, and the

96 VAMP8(-/-) acini are resistant to secretory inhibition by supramaxi

97 ssion in single cells in organotypic mammary acini as a model to elucidate the processes by which onc

98 n per molecule for each protein in mature 3D acini as compared to 2D monolayers.

99 r expression is inhibited in Dicer-deficient acini, as well as in pancreatitis-induced metaplasia.

100 Interacting acini begin to disorganize within 12.5 +/- 4.7 h in a sp

101 As acini begin to form, PTEN accumulates both in the cytopl

102 fibroblast growth factor) to induce mammary acini branching, indicative of a more invasive fibroblas

103 on of SOX2 results in a failure to establish acini but not ducts.

104 sitive myoepithelial cells were found in the acini but not in the normal ducts or dacryops epithelium

105 construction and quantitative study of whole acini by image analysis and stereologic methods, yieldin

106 Inhibition of miRNA synthesis in acini by inactivation of Dicer and pancreatitis-induced

107 Thus, pairs or groups of mammary acini can interact mechanically over long distances thro

108 ial tissues such as kidney tubules or breast acini, cells organize into bidimensional monolayers expe

109 xin to cleave VAMP2 in VAMP8 knock-out (-/-) acini confirmed that VAMP2 and -8 are the primary VAMPs

110 nesis to form a complex secretory organ with acini connected to an extensive ductal system.

111 tioning units (hepatic plates and pancreatic acini) connected to the ductal tree.

112 as was acinar metaplasia in which individual acini consisted of acinar cells and duct-like cells.

113 The resulting acini contained prominent central lumina not observed wh

114 In AR42J and primary acini, CSE+EtOH induced cell death (necrosis and apoptos

115 Cell lines and acini cultures were incubated with IL22 and pharmacologi

116 with ex vivo three-dimensional organoid and acini cultures, we identify new roles for paxillin in th

117 ne-treated primary three-dimensional mammary acini cultures.

118 found that cyclin E deregulation in mammary acini decreases, in an E2F-independent manner, expressio

119 The phenotype of Dicer-deficient acini depends on the induction of HNF6; overexpression o

120 Intriguingly, more than 80% of regenerated acini derive from differentiated cells, including myoepi

121 ated invasiveness and disrupted formation of acini despite continued E-cad expression.

122 that do not interact mechanically with other acini disorganize more slowly (in 21.8 +/- 4.1 h) and to

123 ar morphogenesis, and induction in preformed acini disrupted the pre-established acinar architecture

124 Using the acini-ductal network of the developing human and murine

125 Zymogen activation, observed within acini early during acute pancreatitis for a long time, w

126 Inducible activation of ERK1/2 in mature acini elicits cell motility and disrupts epithelial arch

127 When MYC and Kras(G12D) are induced, the acini enlarge and form solid, depolarized spheres.

128 polarized, growth-attenuated, multicellular acini, enveloped by a continuous endogenous BM.

129 oreactivity for both InvD1L and PDF (type II acini exclusively) revealed positive axons radiating alo

130 titis, Munc18c-depleted (Munc18c(+/-)) mouse acini exhibited a reduction in pathological basolateral

131 easurements of pressure revealed that mature acini experience significant internal hydrostatic pressu

132 As in rodent acini, human acini exposed to toxic concentrations of CCh and tauroli

133 Pancreatic acini express the complexin 2 isoform by RT-PCR and immu

134 In the presence of TN-C, however, acini failed to generate a normal BM, and net epithelial

135 Mammary epithelial acini features were compared using OP or EP under condit

136 ical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis.

137 e utilizing MCF10A mammary epithelial cells, acini form due to integrin-dependent polarization and su

138 The ectopic expression of Runx2 disrupts acini formation, and electron microscopic ultrastructura

139 premature growth arrest during mammary cell acini formation, whereas Amot130 (S175A)-expressing cell

140 Pancreatic ducts and acini from control mice and early-stage PanINs from KPC

141 Proteomic analysis of acini from donors of diverse ethnicity showed similar pr

142 Acini from each of the three miRNA KO mice produced grea

143 27A, but dependent on Rab27B, as shown using acini from genetically modified mice.

144 Here, we found that pancreatic acini from Munc18c-depleted mice (Munc18c(+/-)) and huma

145 Acini from rat lacrimal gland were isolated by collagena

146 ed as image processing tools to discriminate acini from spheroids without any 3D reconstruction.

147 c acinar ductal metaplasia (ADM), pancreatic acini from wild type and KO mice were plated on collagen

148 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechani

149 ve transcriptome analysis of human prostatic acini generated in a three-dimensional basement membrane

150 t Notch and ErbB1/2 both play a role in DCIS acini growth and stem cell activity.

151 As in rodent acini, human acini exposed to toxic concentrations of CC

152 nome-wide mRNA expression analysis of MCF10A acini identified 158 genes regulated by the mutant MYC a

153 ast cells leads to the formation of abnormal acini in 3D culture, but does not promote cell migration

154 : They exclude Hoechst dye 33342, and reform acini in 3D cultures and repopulate mammary fat pads mor

155 ed by the disruption of the morphogenesis of acini in a physiologically relevant three-dimensional mo

156 r in number and larger in size compared with acini in controls.

157 cell proliferation of mammary 3-dimensional acini in culture.

158 suggests its role in water uptake by type I acini in desiccated ticks.

159 t of variation in flow magnitudes across all acini in each model.

160 Further contrasting with EP, acini in OP displayed cooperation between ErbB2 signalli

161 uction and enriched the number of functional acini in submandibular glands of irradiated animals and

162 mage processing tools to separate individual acini in the mouse lung.

163 ously, we have shown that patients with >40% acini in the pancreatic transection line are most prone

164 xonal projections exclusively reached type I acini in the SG of both Ixodes species.

165 neutrophils were largely absent around gland acini in the submucosa.

166 Growth of MCF-10A acini in three-dimensional (3D) culture was enhanced upo

167 n promotes a tumor cell phenotype of mammary acini in three-dimensional culture.

168 optosis in 14-3-3 zeta-overexpressing MCF10A acini in three-dimensional cultures.

169 F10A/p65 cells failed to form characteristic acini in three-dimensional Matrigel.

170 Furthermore, the acini in transgenic mice were fewer in number and larger

171 with hollow lumen similar to normal mammary acini in vivo.

172 ifferentiated mammary epithelial structures (acini) in three-dimensional culture triggers the loss of

173 2+]i was measured using an imaging system in acini incubated with fura-2/AM.

174 Analysis of VAMP8(-/-) acini indicated that although stimulated secretion was s

175 hyperproliferative and disorganized mammary acini induced by chronic stimulation of colony-stimulati

176 o mucous cells does not occur, nor are gland acini inflamed with neutrophils.

177 f recombinant complexin 2 into permeabilized acini inhibited Ca(2+)-stimulated secretion in a concent

178 antagonized the ability of Kras to reprogram acini into PDA preneoplastic precursors.

179 lar gland, we show that de novo formation of acini involves induction of cellular plasticity in multi

180 Suprastimulation of pancreatic acini is a well-known model for pancreatitis, and it is

181 is and find that Myc transgene expression in acini is much lower than in unorganized cells.

182 se that internalization of Ad5 into lacrimal acini is through a novel fiber-dependent mechanism that

183 iated by Na/K-ATPase in type III and type II acini, is followed by a dopamine-independent resorptive

184 esolve components in the pancreas, including acini, islets, blood vessels, and extracellular matrix.

185 formed ex vivo pancreatitis studies in human acini isolated from cadaveric pancreata from organ donor

186 x (ECM), and the apoptosis of inner cells of acini lacking contact with the ECM.

187 ipase knockouts are lethal, exocrine parotid acini lacking lipases were used to verify the results.

188 n intensified the Vav2-induced disruption of acini, leading to more aggressive cell outgrowth and bra

189 ate glandular differentiation, we identified acini-like PCA and related molecular markers that signif

190 s (MCF-10A) form polarized, growth-arrested, acini-like structures with glandular architecture.

191 mers induced disruption of three-dimensional acini-like structures, only heterodimers promoted invasi

192 helial transition (MET) and re-organise into acini-like structures, reminiscent of those formed by ep

193 expressing this profile, which we designated acini-like tumors, had a significantly lower risk of pos

194 roscopy subsequently revealed that polarized acini lipids were more ordered at the apical membranes c

195 imaging system, [Ca(2+)](i) was measured in acini loaded with fura-2.

196 resorptive function of Na/K-ATPase in type I acini located in the proximal end of the salivary duct.

197 In acini, lowering pHe from 7.6 to 6.8 enhanced secretagogu

198 In conclusion, human cadaveric pancreatic acini maintain physiological functions and have similar

199 To study the cellular biomechanics of acini morphogenesis and homeostasis, we used MDCK-2 cell

200 anned, only the high-risk patients with >40% acini (n = 62) continued in the study to receive in tota

201 nitors that give rise to both new islets and acini normally after birth and after injury (ductal liga

202 In vitro experiments were performed in which acini obtained from wild-type and Gpbar1(-/-) mice were

203 bsent in normal mammary ducts, ductules, and acini of histologically normal breast and scanty in the

204 thin granular salivary gland type II and III acini of Ixodes ricinus female.

205 ducibly express active MLK3 in the preformed acini of MCF10A cells grown in 3D Matrigel.

206 epithelia such as colon and stomach, and the acini of salivary glands and the pancreas.

207 The ducts and acini of the human mammary gland are prototypical hetero

208 l, we found that regression of the secretory acini of the mammary gland was compromised in the absenc

209 is study, the expression in mouse pancreatic acini of two candidate Tre-2/Bub2/Cdc16 (TBC) domain-con

210 an immature stage, without the formation of acini or islets.

211 pherical or cylindrical cellular structures (acini or tubes).

212 reast cancer risk with increasing numbers of acini per lobule (P = .0004).

213 ast Disease Cohort, by determining number of acini per lobule and lobular area.

214 These differences in acini phenotype observed between OP and EP highlight the

215 lated rat hepatocyte couplets and pancreatic acini, plus SkHep1 cells as nonpolarized controls.

216 Human acini preferentially expressed the muscarinic acetylchol

217 eliance on rodent models employing dispersed acini preparations.

218 f mutant mice was hypoplastic and individual acini produced few zymogen granules.

219 olarity in live breast glandular structures (acini) produced in three-dimensional cell culture.

220 o-basal polarity in normal breast epithelial acini requires a balance between cell proliferation, cel

221 basement membrane around mature, nonrotating acini restored rotational movement and the ability to as

222 -related C3 botulinum substrate) in ILK-null acini restored the lactation defect, indicating that RAC

223 The reduced Ca(2+) influx in TRPC3(-/-) acini resulted in reduced frequency of the physiologic C

224 5 penton base, fiber, and knob with lacrimal acini revealed that the penton base capsid protein remai

225 ons between acini were cut with a laser, the acini reverted to a slowly disorganizing phenotype.

226 l progenitors forming a network of ducts and acini (secretory cells).

227 In WT acini, short term (14-16 h) culture also results in a >9

228 VAMP8(-/-) acini show increased expression of the endosomal protein

229 ro studies using freshly prepared pancreatic acini show that genetic deletion of PAR2 reduces bile sa

230 of Ca2+ signaling in wild-type and Trpc3-/- acini showed that Pyr3 is a highly specific inhibitor of

231 this study we demonstrate that DAPT reduced acini size and mammosphere formation in MCF10DCIS.com wh

232 Lapatinb reduced acini size and mammosphere formation in SUM225, whereas

233 nib treatment was more effective at reducing acini size in both DCIS cell lines.

234 Glandular tissues form ducts (tubes) and acini (spheres) in multicellular organisms.

235 Addition of fibronectin to differentiated acini stimulated proliferation and reversed growth arres

236 e mechanisms by which 14-3-3 zeta alters MEC acini structure and increases the risk of breast cancer.

237 nced proliferation and formation of aberrant acini structure in the three-dimensional culture.

238 Characterization of the disrupted acini structures showed increased cell proliferation (Ki

239 h in a spatially coordinated manner, whereas acini that do not interact mechanically with other acini

240 s, and that an inverse situation occurred in acini that lost apical polarity upon treatment with Ca(2

241 Here we use freshly isolated pancreatic acini that preserve the luminal structure to measure int

242 epithelial ductal networks that terminate in acini that together produce, transport and secrete saliv

243 qui parasites in the salivary gland granular acini, the parasites expressed levels of paralogous surf

244 In type I acini, the rich network of cholinergic axons terminate w

245 specificity of targeting in 3D multicellular acini, these findings are promising and the approach mer

246 may inhibit anoikis and luminal clearance in acini through induction of autophagy.

247 ole for peripheral nerves in the creation of acini throughout development via regulation of SOX2.

248 Exposure of the acini to acetaldehyde and ethyl oleate followed by CCK-8

249 ress their 3D morphology from that of hollow acini to branched structures characteristic of nonmetast

250 Leung and Brugge use cultured breast cancer acini to demonstrate the importance of local interaction

251 Exposure of pancreatic acini to ethanol blocks cholecystokinin (CCK)-8-stimulat

252 Exposure of salivary gland cells and acini to hypotonicity elicited an increase in cell volum

253 ubnuclear targeting resulted in reversion of acini to more normal structures and reduced tumor growth

254 In both acini types, SIFamide-positive axons were found to be in

255 minating on specific cells of salivary gland acini (types II and III).

256 small interfering RNA (siRNA) into lacrimal acini under conditions that reduced intracellular CAR mR

257 of this secretory blockade, Munc18c-depleted acini unexpectedly activated a component of the endoplas

258 holecystokinin (CCK) was also seen in intact acini using immunofluorescence, in a biotinylated fracti

259 enchymal interdependence between neighboring acini was accounted for.

260 During the operation, the percentage of acini was calculated from pancreatic transection line fr

261 ced mucin secretion by rat sublingual tubulo-acini was dependent upon PLC activation and the subseque

262 list of 10,294 genes expressed in ducts and acini was searched using gene ontologies related to ion

263 lux of fluid through the epithelial cells of acini, we propose that complex control of the tick saliv

264 Cultured Bmi1(-/-) and wild-type primary acini were analyzed in vitro to determine acinar-specifi

265 Acini were collected from C57BL/6 mice and resected huma

266 the directed mechanical connections between acini were cut with a laser, the acini reverted to a slo

267 When acini were fully mechanically isolated from other acini

268 Acini were incubated with adenoviruses overnight or the

269 Rat pancreatic acini were incubated with concentrations of ethanol asso

270 Rat lacrimal gland acini were incubated with H89, an inhibitor of protein k

271 Acini were incubated with the fluorescence indicator fur

272 Acini were incubated with the fluorescent indicator mole

273 Lacrimal gland acini were isolated by collagenase digestion and incubat

274 Rat lacrimal gland acini were isolated by collagenase digestion.

275 Primary acini were isolated from mice and analyzed in amylase se

276 Acini were preincubated with the PKC inhibitors calphost

277 Acini were stimulated with the P2X(7) receptor agonist,

278 Acini were stimulated with the P2X7 receptor agonist, (b

279 gene expression was again seen when the HMEC acini were sub-cultured as a monolayer, implying that lr

280 Permeabilized acini were used to understand the differential role of c

281 F10A cell line differentiates to form hollow acini when grown in Matrigel, and expression of LMP2A in

282 ng morphogenesis in human mammary epithelial acini when grown in three-dimensional cultures and are a

283 le adult epithelial cells generate polarized acini when placed in a surrogate basement membrane 3D ge

284 form polarized, growth-arrested structures (acini) when cultured in three-dimensional laminin-rich e

285 gy based on primary mouse mammary epithelial acini, where oncogenes can be switched on in single cell

286 cytic foci and areas of altered or distorted acini, whereas the ID/Rx group had scattered small lymph

287 ency factors (Sox9 and Hnf1beta) in Ptf1a(+) acini, which undergo rapid reprogramming to duct cells a

288 age-independent conditions and form aberrant acini, which was dependent on Ad-cyclin E or Ad-LMW-E ex

289 and other neoplastic-like changes in mammary acini, while silencing alphaB-crystallin by RNA interfer

290 populations serving as a reserve source for acini widens the therapeutic options for hyposalivation.

291 f Ad5 with excess heparin or pretreatment of acini with a heparinase cocktail each inhibited Ad5 tran

292 Here we show that mammary acini with compromised structural integrity can intercon

293 sis in the luminal area, resulting in bigger acini with filled lumens.

294 ccurs in a 3D coculture system that combines acini with human mammary adipose tissue, and establish t

295 rostate epithelial cells that form polarized acini with lumen under standard tridimensional (3D) cult

296 Pretreatment of acini with Src inhibitors or expression of a cortactin t

297 ls retained the capacity to generate mammary acini within extracellular matrix.

298 evelopment of hyperplastic three-dimensional acini without affecting apical-basal polarity.

299 CF10A cells formed irregular and near-normal acini without hollow lumen in three-dimensional culture.

300 is is caused by an increase in the number of acini without individual acinar cell hyperplasia.