ライフサイエンスコーパス: acrosome

1 flagellar midpiece and of ENaC-delta to the acrosome.

2 n on the sperm plasma membrane overlying the acrosome.

3 artial co-localization with a marker for the acrosome.

4 isplay round-headed spermatozoa that lack an acrosome.

5 7 to the M1 (dorsal bulge) domain within the acrosome.

6 apical segment of the cauda epididymal sperm acrosome.

7 nucleus in a wave following the progressing acrosome.

8 es of the sperm including the absence of the acrosome.

9 ng protein TMED7/p27 are segregated from the acrosome.

10 irmed the presence of PSMA1-GFP in the sperm acrosome.

11 ein in both the flagella/neck region and the acrosome.

12 aining zona-binding molecules over the sperm acrosome.

13 act CatSper channel, lack of pY, and reacted acrosomes.

14 is recommended for animals with large sperm acrosomes.

15 we show that the protein is localized to the acrosome, a membrane-bound vesicle located at the apical

16 Secretion of the acrosome, a single vesicle located rostrally in the head

17 rmiogenesis, TPCL became associated with the acrosome, a sperm-specific organelle, along with a subse

18 others (GBF1, GPP34, GRASP55) remain in the acrosome and are progressively lost in later steps of di

19 is revealed caveolin-1 in the regions of the acrosome and flagellum in sperm of both species.

20 t mice that exhibit spermiogenic arrest with acrosome and flagellum malformation.

21 spermiogenic mRNAs including those encoding acrosome and flagellum proteins.

22 (GBF1, GPP34, GRASP55) localize to both the acrosome and Golgi, while GL54D, TM9SF3, and the Golgi t

23 rm acrosomal matrix is the dense core of the acrosome and is likely to be important in acrosome bioge

24 Electron microscopy revealed abnormal acrosome and manchette and the absence of implantation f

25 Vs bound and fused with the membranes of the acrosome and mid piece; and (3) incubating sperm with EV

26 ogenesis, basonuclin also accumulated in the acrosome and mitochondrial sheath surrounding the flagel

27 sely associated with the leading edge of the acrosome and to the nuclear envelope during the elongati

28 yntrophin in cerebral vasculature, spermatic acrosomes and neuronal processes.

29 remodeling of the sperm plasma membrane and acrosome, and for the acquisition of sperm fertilizing a

30 arge round heads (globozoospermia), abnormal acrosomes, and defective mobility.

31 sperm that the plasma membrane overlying the acrosome (APM) contains several types of microdomains kn

32 mponents from the genome via the progressing acrosome as transcription is repressed and chromatin is

33 l deubiquitinating enzyme that has a role in acrosome assembly; UBD and EPSTI1, which have potential

34 nzymes on embryo development, the removal of acrosomes before ICSI is recommended for animals with la

35 d to be freed from acrosomes, the removal of acrosomes before ICSI is theoretically preferable.

36 appen when these spermatozoa were freed from acrosomes before ICSI, regardless of the number of sperm

37 not happen when spermatozoa were freed from acrosomes before injection.

38 he acrosome and is likely to be important in acrosome biogenesis and fertilization.

39 ults suggest that PRAMEL1 may play a role in acrosome biogenesis and sperm motility.

40 work has uncovered a crucial role of NHE8 in acrosome biogenesis and suggests that some forms of huma

41 nd/or fusion of proacrosomic vesicles during acrosome biogenesis.

42 this article, we stall the extension of the acrosome bundle in agarose gels of different concentrati

43 hus, the elastic strain energy stored in the acrosome bundle is more than sufficient to power the acr

44 olecules localize to the apical ridge on the acrosome but following cholesterol efflux with methyl-be

45 fusion occurs at multiple points within the acrosome but how fusion is activated and the formation a

46 ody 7C5 detected sp56 within the mouse sperm acrosome, but failed to detect sp56 on the surface of ac

47 cytodifferentiation, forming sperm axonemes, acrosomes, centrosome nuclear binding, transient manchet

48 ria-derived membranes are assembled into the acrosome, challenging the concept that this organelle is

49 trated that absence of ZPBP1 prevents proper acrosome compaction, resulting in acrosome fragmentation

50 rane within oocytes and incorporation of the acrosome containing a spectrum of hydrolyzing enzymes.

51 abundantly associated with the tail and the acrosome-containing head region of mature sperm.

52 concentration, impaired motility and higher acrosome damage were demonstrated in SHRs.

53 on sperm concentration and motility but not acrosome damage.

54 he neuromuscular secretory vesicle and sperm acrosome defects, other developmental abnormalities and

55 b, the vesicles are unable to fuse, blocking acrosome development at step 2.

56 us to follow the fate of the protein and the acrosome during fertilization.

57 of stabilizing and anchoring the developing acrosome during spermatid nuclear elongation.

58 fied in sperm from subfertile stallions with acrosome dysfunction.

59 fflux and the ganglioside GM1 regulate sperm acrosome exocytosis (AE) and fertilization competence th

60 ment for sterol removal for sperm to undergo acrosome exocytosis (AE), the mechanisms by which change

61 P3) surrounding ovulated mouse eggs, undergo acrosome exocytosis and penetrate the zona matrix before

62 to fertilize eggs, although capacitation and acrosome exocytosis appear to be normal.

63 mCherry-labeled acrosomes failed to undergo acrosome exocytosis efficiently in the micropyle, with b

64 e form) added to permeabilized sperm induces acrosome exocytosis even in the absence of extracellular

65 mammalian sperm, through a process known as "acrosome exocytosis" (AE), is essential for fertilizatio

66 ase of neurotransmitters and hormones, sperm acrosome exocytosis, plasma membrane repair and neurite

67 f the zona is not sufficient to induce sperm acrosome exocytosis.

68 e zona pellucida is not sufficient to induce acrosome exocytosis.

69 transgenic mouse sperm with mCherry-labeled acrosomes failed to undergo acrosome exocytosis efficien

70 inated with morphological alterations during acrosome formation in spermatids, and were significantly

71 After acrosome formation, GL54D, TM9SF3, TMED4/p25, and TMED7/

72 During acrosome formation, several Golgi proteins (GBF1, GPP34,

73 ILT are also Golgi recruited but only during acrosome formation.

74 number, reduced sperm motility and defective acrosome formation.

75 nts proper acrosome compaction, resulting in acrosome fragmentation and disruption of the Sertoli-spe

76 late the characteristics of Drosophila Snky, acrosome function and sperm PMBD to membrane fusion even

77 zoa and the mobilization of calcium from the acrosome has been determined only partially.

78 The persistence of intact acrosomes indicates that sperm binding to the zona pellu

79 We provide evidence that the acrosome induces changes in sperm plasma membrane, exclu

80 Instead, acrosome intact mouse sperm were briefly fixed in 1% glu

81 ine the lateral mobility of fertilin in both acrosome-intact and acrosome-reacted sperm.

82 osis efficiently in the micropyle, with both acrosome-intact and reacted sperm found in the inter-cho

83 In conclusion, acrosome-intact boar sperm exhibit concentrated zona pro

84 t the surface of the zona pellucida remained acrosome-intact for more than 2 hours and were displaced

85 Injection of a single acrosome-intact hamster, bovine, and porcine spermatozoo

86 e show that complexins I and II are found in acrosome-intact mature sperm, bind to SNARE complex prot

87 but failed to detect sp56 on the surface of acrosome-intact mouse sperm.

88 PH-20 hyaluronidase activity indicated that acrosome-intact sperm exhibited two peaks of hyaluronida

89 The majority of enzyme activity in acrosome-intact sperm extracts occurred at neutral pH, w

90 Western blots of SDS-PAGE of acrosome-intact sperm extracts revealed a 64-kDa form of

91 Zona proteins bound live, acrosome-intact sperm on the anterior portion of the spe

92 lateral mobility of fertilin in capacitated, acrosome-intact sperm revealed two populations of cells.

93 ep in murine fertilization is the binding of acrosome-intact sperm to specific O-linked oligosacchari

94 sin and hyaluronidase mimicked the action of acrosome-intact spermatozoa, it is likely that the acros

95 e hatching rate of females and decreased the acrosome integrity and sperm motility of males.

96 A greater (P < 0.05) percentage of acrosome integrity in frozen-thawed spermatozoa was obse

97 rvation and post-thaw incubation, as well as acrosome integrity post-thaw.

98 eters, without modifying sperm viability and acrosome integrity.

99 protective effect on the sperm membrane and acrosome integrity.

100 Surprisingly, we find that the Drosophila acrosome is a paternally inherited structure.

101 In many animals, the acrosome is a secretory vesicle with exocytosis essentia

102 The acrosome is an acidic secretory vesicle containing hydro

103 ic egg recognition by a protein in the sperm acrosome is conserved between invertebrates and vertebra

104 The sperm acrosome is essential for sperm-egg fusion and is often

105 ghtly shorter head, likely as a result of an acrosome lacking Bindin.

106 Oocytes did not deform and lyse when acrosome-less spermatozoa were injected, regardless of t

107 The removal of acrosomes may increase the efficiency of ICSI in these a

108 functionality, viability, total abnormality, acrosome membrane integrity, mitochondrial activity, rea

109 ffected spermatozoa had disrupted plasma and acrosome membranes, and mitochondrial membrane depolariz

110 m sensor synaptotagmin I, are present in the acrosome of mammalian sperm (human, rhesus monkey, bull,

111 min GTPase family localize to the developing acrosome of maturing mouse germ cells.

112 between 40 and 60 uM rosiglitazone in intact acrosome of ram thawed semen.

113 ining indicated that FNDC3A localizes to the acrosome of spermatids, as well as to Leydig cells in th

114 ) exchanger NHE8 localizes to the developing acrosome of spermatids.

115 Zona binding proteins were present in the acrosomes of sperm from all regions of the epididymis.

116 Surprisingly, the acrosomes of the bound sperm remain intact for at least

117 Sperm acrosomes of the Pacific oyster Crassostrea gigas contai

118 ential abundance proteins, including some of acrosome origin, were identified in sperm from subfertil

119 he acroplaxome, the cytoskeletal base of the acrosome, previously linked to chromatin reorganization.

120 the finding that sp56 is present within the acrosome provides further support that sp56 and AM67 are

121 When Crassostrea gigas oyster sperm acrosome react a ring of bindin protein is exposed that

122 Although complexin-I-deficient sperm acrosome-react in response to calcium ionophore, they do

123 n response to calcium ionophore, they do not acrosome-react in response to egg zona pellucida protein

124 tion of these proteins and the percentage of acrosome reacted sperm can be regulated by PKA modulator

125 In capacitated and acrosome reacted sperm, fusion was significantly (p < 0.

126 proteins in the acrosomal matrix that retain acrosome reacted spermatozoa on the zona surface prior t

127 Capacitated, acrosome-reacted sperm and zona-free eggs were used in a

128 mechanisms by which fertilization competent acrosome-reacted sperm bind to the oolemma remain unchar

129 In vitro binding of porcine acrosome-reacted sperm cells to oocytes was found to be

130 Acrosome-reacted sperm demonstrated SAMP14 immunofluores

131 PUFAs, whereas the amount of cholesterol in acrosome-reacted sperm displayed a 1.7-fold increase.

132 tein IZUMO1 on the equatorial segment of the acrosome-reacted sperm recognizes its receptor, JUNO, on

133 ighly conserved mammalian protein present on acrosome-reacted sperm that is thought to promote fertil

134 Indirect immunofluorescence on live, acrosome-reacted sperm using antibodies against either c

135 However, fertilin in acrosome-reacted sperm was highly mobile within the memb

136 somal localization, Gs reactivity is lost in acrosome-reacted sperm, and forskolin is able to increas

137 protein exposed on the equatorial segment of acrosome-reacted sperm, and the egg plasma-membrane-anch

138 ntion of mSLLP1 in the equatorial segment of acrosome-reacted sperm, the inhibitory effects of both r

139 lity of fertilin in both acrosome-intact and acrosome-reacted sperm.

140 d glycans isolated from the receptor bind to acrosome-reacted sperm.

141 licular fluid, we unexpectedly observed that acrosome-reacted spermatozoa activated the complement ca

142 forms of native Sp17 remain associated with acrosome-reacted spermatozoa and are solubilized by ioni

143 17) can also be proteolytically processed by acrosome-reacted spermatozoa in a similar manner.

144 ifunctional protein that mediates binding of acrosome-reacted spermatozoa to zona glycoproteins via a

145 taining an alpha7 subunit in the human sperm acrosome reaction (a modified exocytotic event essential

146 The sea urchin sperm acrosome reaction (AR) is a prerequisite for sperm-egg f

147 The mammalian sperm acrosome reaction (AR) is essential to fertilization, an

148 ilization, egg jelly (EJ) triggers the sperm acrosome reaction (AR) which is required for sperm bindi

149 The mammalian sperm acrosome reaction (AR), an essential fertilization event

150 Sea urchin egg jelly (EJ) triggers sperm acrosome reaction (AR), an exocytotic event required for

151 FSP induces the sperm acrosome reaction (AR), an exocytotic process required f

152 During fertilization, the sea urchin sperm acrosome reaction (AR), an ion channel-regulated event,

153 Intact sperm were evaluated before the acrosome reaction (AR), and a soluble form of PH-20 rele

154 y has been shown to be involved in the sperm acrosome reaction (AR), but the molecular identity of PL

155 One event, the sea urchin sperm acrosome reaction (AR), is blocked by the lectin wheat g

156 The exocytotic acrosome reaction (AR), which is required for fertilizat

157 (ZP) via sperm receptor(s) and undergoes an acrosome reaction (AR).

158 ptide is able to induce sperm to undergo the acrosome reaction (i.e., cellular exocytosis) in vitro.

159 atorial segment of spermatozoa following the acrosome reaction and a role for mSLLP1 in sperm-egg bin

160 ability of the zona pellucida to induce the acrosome reaction and by successful fertilization in vit

161 ertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the egg.

162 tents, and SNARE antibodies inhibit both the acrosome reaction and fertilization, without inhibiting

163 rane of the human spermatozoan following the acrosome reaction and may play a role in fertilization.

164 and sufficient to prepare the sperm for the acrosome reaction and suggest that changes in sperm memb

165 c inhibition also blocks the agonist-induced acrosome reaction and that this inhibition is overcome b

166 itation, hyperactivation of motility and the acrosome reaction are all mediated by increases in intra

167 ZP3 binding and subsequent induction of the acrosome reaction are dispensable for fertilization, the

168 ne phosphorylation and the ionophore-induced acrosome reaction as well as luteinizing hormone, follic

169 concentrations inhibited the agonist-induced acrosome reaction as well as the increase in [Ca(2+)](i)

170 It has been suggested that ZP3 induces the acrosome reaction by crosslinking GalTase, activating a

171 Induction of the acrosome reaction does not appear to alter the molecular

172 s by endogenous sialidases after a premature acrosome reaction during acute epididymitis.

173 glycoprotein, ZP3, and as an inducer of the acrosome reaction following ZP3-dependent aggregation.

174 Cells undergoing an acrosome reaction in aggregations remote from the egg ar

175 (2+) channels, inhibits progesterone-induced acrosome reaction in human sperm, but fluorimetric studi

176 ld-type sperm, and are unable to undergo the acrosome reaction in response to either ZP3 or anti-gala

177 asting conditions, autophagy regulation, the acrosome reaction in sperm, cancer cell migration, and i

178 ation of hundreds of fusion pores during the acrosome reaction in spermatozoa and the mobilization of

179 actions during and immediately following the acrosome reaction in the mouse.

180 r activity) and induces sperm to undergo the acrosome reaction in vitro at about the same concentrati

181 tion was sufficient to prepare sperm for the acrosome reaction induced either by depolarization with

182 The sperm acrosome reaction is a Ca(2+)-dependent exocytotic event

183 The sperm acrosome reaction is a Ca(2+)-dependent secretory event

184 The acrosome reaction is a unique exocytotic event involving

185 In contrast, gt(-/-) sperm undergo the acrosome reaction normally in response to calcium ionoph

186 Motility, chemotaxis, and the acrosome reaction of animal sperm are all regulated by c

187 During the 5 s of the acrosome reaction of Limulus polyphemus sperm, a 60-micr

188 The metabolism, flagellar beating, and acrosome reaction of spermatozoa are regulated by ion fl

189 o resulted in spermatozoa displaying reduced acrosome reaction potential.

190 Mammalian spermatozoa must complete an acrosome reaction prior to fertilizing an oocyte.

191 ty of gt(-/-) sperm to undergo a ZP3-induced acrosome reaction renders them physiologically inferior

192 bundle is more than sufficient to power the acrosome reaction through the egg envelope.

193 decreased the effect of progesterone on the acrosome reaction to control levels.

194 alyzed for its involvement in triggering the acrosome reaction using a TPCN1 gene-deficient mouse str

195 ults in the release of SNAP-25 with the shed acrosome reaction vesicles.

196 On the other hand, when the acrosome reaction was induced, either with progesterone

197 Because a premature acrosome reaction was observed in an UPEC epididymitis m

198 populations, unlike the previously proposed "acrosome reaction" model.

199 released by regulated exocytosis (termed the acrosome reaction) during fertilization or on exposure t

200 one causes a single large transient (causing acrosome reaction) which is apparently dependent upon Ca

201 etected in sperm after acrosomal exocytosis (acrosome reaction).

202 During the acrosome reaction, a 60-microm long coiled and twisted b

203 Because the acrosome reaction, a prelude to binding, is known to be

204 ion occurs after the completion of the sperm acrosome reaction, a secretory event that is triggered d

205 da glycoproteins, and once bound undergo the acrosome reaction, a type of cellular exocytosis.

206 This membrane is exposed after the acrosome reaction, an exocytosis event that occurs upon

207 arization, which is required for the ensuing acrosome reaction, an exocytotic process essential for f

208 in male fertility, including sperm motility, acrosome reaction, and embryonic development.

209 hyperactivation, the zona pellucidae-induced acrosome reaction, and most importantly, fertilization w

210 to bind soluble ZP3, undergo the ZP3-induced acrosome reaction, and penetrate the zona pellucida.

211 lar Ca(2+) leading to sperm hyperactivation, acrosome reaction, and perhaps chemotaxis toward the egg

212 m motility, chemotaxis, capacitation and the acrosome reaction, and play a vital role in the ability

213 the sperm's ability to swim and undergo the acrosome reaction, and thus redistribution of surface pr

214 logy, ability to undergo capacitation or the acrosome reaction, and/or mitochondrial membrane potenti

215 we show that Rab27 is also required for the acrosome reaction, as demonstrated by the inability of i

216 Acrosome reaction, binding to zona pellucida and fusion

217 [Ca(2+)](i) oscillations did not induce acrosome reaction, but in cells generating oscillations,

218 e ability to undergo a zona pellucida-evoked acrosome reaction, develops more slowly in sperm from Pk

219 be involved in sperm capacitation and/or the acrosome reaction, essential steps in fertilization wher

220 sociated with capacitation, induction of the acrosome reaction, forward velocity, or percentage of mo

221 imals must complete an exocytotic event, the acrosome reaction, in order to fuse with eggs.

222 The steroid progesterone, an agonist of acrosome reaction, induces a biphasic [Ca(2+)](i)-signal

223 by removing a C-terminal fragment during the acrosome reaction, might be a mechanism to regulate the

224 Sperm SNAREs are sloughed off during the acrosome reaction, paralleling the release of sperm memb

225 lvement of SNAP-25 in the invertebrate sperm acrosome reaction, possibly through increased associatio

226 However, during the acrosome reaction, Sp17 is processed from a 22- to 24-kD

227 lation inhibited the acetylcholine-initiated acrosome reaction, suggesting the involvement of a SRC k

228 lthough this complex is present prior to the acrosome reaction, the amount of complex increases over

229 or sperm motility and ability to undergo the acrosome reaction, two processes central to oocyte ferti

230 posterior head plasma membrane following the acrosome reaction, when it functions in sperm-egg intera

231 dergoing hyperactivated motility and induced acrosome reaction, which are necessary to fertilize an e

232 coat of the egg (egg jelly), triggering the acrosome reaction, which transforms the sperm into a fus

233 volves multiple ordered steps, including the acrosome reaction, zona pellucida penetration, sperm-egg

234 ibutes of human sperm function: motility and acrosome reaction.

235 signal transduction necessary to trigger the acrosome reaction.

236 ractivated motility, and are readied for the acrosome reaction.

237 mines their expansion and the success of the acrosome reaction.

238 sting the involvement of a SRC kinase in the acrosome reaction.

239 n the inner acrosomal membrane following the acrosome reaction.

240 ed on the inner acrosomal membrane after the acrosome reaction.

241 ucida and undergo a Ca(2+) ionophore-induced acrosome reaction.

242 lation as well as the zona pellucida-induced acrosome reaction.

243 st of the spermatozoa undergoing a premature acrosome reaction.

244 previously characterized involvement in the acrosome reaction.

245 at is released from abalone sperm during the acrosome reaction.

246 pathway and that this drives the exocytotic acrosome reaction.

247 and active in sperm after activation by the acrosome reaction.

248 sion events during fertilization, namely the acrosome reaction.

249 ovide calmodulin at specific sites after the acrosome reaction.

250 sicle, whose contents are exposed during the acrosome reaction.

251 a role for these proteins in regulating the acrosome reaction.

252 gone associative changes during or after the acrosome reaction.

253 hange in twist of actin filaments during the acrosome reaction.

254 rm to eggs or to induce sperm to undergo the acrosome reaction.

255 d capacity to undergo a progesterone-induced acrosome reaction.

256 the sperm for an exocytotic process known as acrosome reaction.

257 increase intracellular Ca(2+) and induce the acrosome reaction.

258 hyperactivated motility, chemotaxis, and the acrosome reaction.

259 and are thus well positioned to regulate the acrosome reaction.

260 dent EGFR activation, Ca(2+) influx, and the acrosome reaction.

261 is key to enabling mice sperm to undergo the acrosome reaction.

262 ly to ZP3 and fail to undergo a zona-induced acrosome reaction; however, they still bind to the ovula

263 ylinositol-3-kinase antagonists that prevent acrosome reactions and fertilization in vitro, while gen

264 In mammals, acrosome reactions are triggered during sperm contact wi

265 membranes were permeabilized by fixation or acrosome reactions induced by the ionophore A23187, zona

266 y increased sensitivity to ionophore-induced acrosome reactions without undergoing capacitation in vi

267 phoinositide in the absence of ZP3 triggered acrosome reactions.

268 the zona pellucida (ZP), and fewer underwent acrosome reactions.

269 a role in ZP3-evoked Ca2+ influx that drives acrosome reactions.

270 2+) concentration in mouse sperm, leading to acrosome reactions.

271 a role of voltage-sensitive Ca2+ channels in acrosome reactions.

272 ective fluorescent probes, and also inhibits acrosome reactions.

273 ssive motility, hyperactivated motility, and acrosome reactions.

274 erm membrane caused breakage of the neck and acrosome region and immotility of sperm.

275 rtion of sperm displaying ARSF signal at the acrosome region was observed in sperm from subfertile Th

276 Its localization with the acrosome suggests that it may also perform a special fun

277 calized to the plasma membrane overlying the acrosome, supportive of a role in cell adhesion/signalin

278 e developed a transgenic mouse expressing an Acrosome-targeted Sensor for Exocytosis (AcroSensE) to i

279 Hamsters, cattle, and pigs have much larger acrosomes than the mouse or human.

280 use spermatozoa do not need to be freed from acrosomes, the removal of acrosomes before ICSI is theor

281 ch occurs at a constant velocity, allows the acrosome to forcefully penetrate the egg.

282 signated acroplaxome, anchors the developing acrosome to the nuclear envelope.

283 binant Munc18-1 inhibited the docking of the acrosome to the plasma membrane.

284 se, the removal of sperm plasma membrane and acrosome was not a prerequisite to produce offspring by

285 d spermatozoon protein that localizes to the acrosome, was originally identified in pigs and named fo

286 sidered to be activated via proteases of the acrosome, we aimed to investigate whether alterations of

287 three or more mouse spermatozoa with intact acrosomes were injected into individual mouse oocytes, a

288 atozoa from Nhe8(-/-) mice completely lacked acrosomes, were round-headed, exhibited abnormal mitocho

289 the anterior part of the sperm nucleus, the acrosome, which is essential for male fertility.

290 Golgi identity as it migrates away from the acrosome, while the others (GBF1, GPP34, GRASP55) remain

291 in a large dense-core secretory granule (the acrosome) whose contents are secreted by regulated exocy

292 r, sperm release a single large vesicle, the acrosome, whose release has different characteristics th