ライフサイエンスコーパス: actin-activated ATPase

1 Consistently, ADP decreased K(actin) of the actin-activated ATPase.

2 ctin binding, ATP-induced actin release, and actin-activated ATPase.

3 C to study structural correlates of myosin's actin-activated ATPase.

4 Also, OM reduces maximum actin-activated ATPase 2-fold in F764L, compared with 4-

5 sured basal ATPase, V(max), and K(ATPase) of actin-activated ATPase, actin-sliding velocities, rigor

6 The actin-activated ATPase activities (Vmax) of asymmetric H

7 We found that, based upon the change in the actin-activated ATPase activities and actin translocatin

8 tions in a manner that reflects the relative actin-activated ATPase activities of the donor myosins.

9 Similarly, actin-activated ATPase activities of the mutant and wild

10 We have studied the actin-activated ATPase activities of three mutations in

11 e domain sequence switch conferred beta-like actin-activated ATPase activities to the chimeric myosin

12 Actin-activated ATPase activities were also reduced.

13 chains of amoeba myosins I, increasing their actin-activated ATPase activities.

14 n vitro motility (2.7-fold), and the Vmax of actin-activated ATPase activity (up to 2-fold), the prop

15 n comprising an N-terminal motor domain with actin-activated ATPase activity and a C-terminal globula

16 these cells exhibited 4-5-fold reduction in actin-activated ATPase activity and a decrease in motor

17 We found calcium moderately increases the actin-activated ATPase activity and completely inhibits

18 lated Myo2 were obtained that exhibited high actin-activated ATPase activity and in vitro actin filam

19 defective properties in vitro, particularly actin-activated ATPase activity and sliding velocity.

20 ila, mouse, and human myosin 18A (M18A) lack actin-activated ATPase activity and the ability to trans

21 Both the actin-activated ATPase activity and the actin translocat

22 Both the actin-activated ATPase activity and the actin-translocat

23 The Ala and Asn mutants had the same low actin-activated ATPase activity as unphosphorylated wild

24 tation does not significantly change maximum actin-activated ATPase activity but slows actin sliding

25 Mg(2+) modulated actin-sliding velocity and actin-activated ATPase activity by changing the rate of

26 2C, A397D, and E450Q mutations abolished the actin-activated ATPase activity completely.

27 The K(m) for actin-activated ATPase activity decreased 15-fold, but V

28 ve for both actin-translocating activity and actin-activated ATPase activity in the dephosphorylated

29 bed here, we have found that the kcat of the actin-activated ATPase activity is changed by the loop 2

30 Muscle myosin-II actin-activated ATPase activity is significantly higher

31 Therefore, the Mg(2+) inhibition of the actin-activated ATPase activity observed in class II myo

32 The actin-activated ATPase activity of Acanthamoeba myosin I

33 The actin-activated ATPase activity of all constructs except

34 ion significantly decreased the K(m) for the actin-activated ATPase activity of both alpha- and beta-

35 e been shown previously to down-regulate the actin-activated ATPase activity of both the full-length

36 Inhibition of actin-activated ATPase activity of fast skeletal and car

37 The actin-activated ATPase activity of full-length mammalian

38 t exogenous GTD is capable of inhibiting the actin-activated ATPase activity of GTD-deleted myosin Va

39 V(max) and K(actin) of the actin-activated ATPase activity of HuM5B were 9.7 +/- 0.

40 o suggest a reciprocal shift, with basal and actin-activated ATPase activity of IFI-3a showing reduce

41 V(max) and K(ATPase) of the actin-activated ATPase activity of M10IQ1 were 13.5 s(-1

42 We demonstrate that the maximum actin-activated ATPase activity of M2beta-S1 is slowed m

43 In EGTA, the actin-activated ATPase activity of MD(2IQ) was 7.4 +/- 1

44 ation significantly decreased the Km for the actin-activated ATPase activity of MHC isoforms.

45 , RLC phosphorylation does not influence the actin-activated ATPase activity of Myo2p.

46 Interestingly, the actin-activated ATPase activity of MYO3A 2IQ is slightly

47 at the inhibitory action of caldesmon on the actin-activated ATPase activity of myosin in solution an

48 The actin-activated ATPase activity of myosin increased sign

49 und that melanophilin directly activates the actin-activated ATPase activity of myosin Va and thus it

50 ADP did not significantly inhibit the actin-activated ATPase activity of myosin X, suggesting

51 The steady-state actin-activated ATPase activity of NMIIB S1 was decrease

52 tern blot analysis (116 kDa) and inhibit the actin-activated ATPase activity of purified adrenal myos

53 The actin-activated ATPase activity of smooth muscle myosin

54 ingly, the tail domain markedly inhibits the actin-activated ATPase activity of tailless DM7A at low

55 On the other hand, the maximum actin-activated ATPase activity of the chimeric myosin w

56 om the wild-type ABL significantly increased actin-activated ATPase activity of the dephosphorylated

57 Recent studies have revealed that the actin-activated ATPase activity of the full-length myosi

58 The actin-activated ATPase activity of the melanocyte-type m

59 large as that of the monomer form, while the actin-activated ATPase activity of the two forms was ide

60 a as determined by (i) the dependence of its actin-activated ATPase activity on heavy-chain phosphory

61 e E56G mutation has no significant effect on actin-activated ATPase activity or actomyosin affinity i

62 ble for USH1B cause the complete loss of the actin-activated ATPase activity or the reduction of duty

63 (1) Both the actin sliding activity and the actin-activated ATPase activity showed phosphorylation d

64 ATPase activity for 2-3-fold but reduced the actin-activated ATPase activity to 50% of the wild type.

65 urements at the same temperature showed that actin-activated ATPase activity was 2.9-fold greater for

66 slocating activity of myosin VI although the actin-activated ATPase activity was not affected by Ca(2

67 The actin-activated ATPase activity was reduced at Ca(2+) co

68 K(actin) and K(ATP) of the actin-activated ATPase activity were significantly highe

69 the 50-20K sequence specifically affects the actin-activated ATPase activity while the 25-50K sequenc

70 Q mutant myosin demonstrated 2.3-fold higher actin-activated ATPase activity, 2.2-fold greater averag

71 IFI relay domain, we find a 50% reduction in actin-activated ATPase activity, a significant increase

72 nd actin in an ATP-sensitive manner, exhibit actin-activated ATPase activity, and generate force and

73 unts for the low actin-sliding velocity, low actin-activated ATPase activity, and high duty ratio.

74 ted the overall shape of a myosin, exhibited actin-activated ATPase activity, and moved actin filamen

75 We subsequently found that piglet had an actin-activated ATPase activity, colocalized with actin

76 he N-dependence of actin sliding velocities, actin-activated ATPase activity, force generation agains

77 I455M has normal actin-activated ATPase activity, Pi burst, and ATP-induc

78 nine and glutamic acid substitutions reduced actin-activated ATPase activity, slowed the in vitro sli

79 n the other hand, ADP markedly inhibited the actin-activated ATPase activity, suggesting a high affin

80 All three mutants, however, have impaired actin-activated ATPase activity, with apparent second-or

81 of motility and nearly 5-fold regulation of actin-activated ATPase activity.

82 es for inhibitors of the recombinant motor's actin-activated ATPase activity.

83 main constructs (amino acids 1-800) featured actin-activated ATPase activity.

84 influence actin binding and thereby modulate actin-activated ATPase activity.

85 ), which was comparable to the V(max) of the actin-activated ATPase activity.

86 ADP markedly inhibited the actin-activated ATPase activity.

87 ng protein that possesses calcium-stimulated actin-activated ATPase activity.

88 ffects ADP release, while loop 2 affects the actin-activated ATPase activity.

89 with actin, and display wild-type levels of actin-activated ATPase activity.

90 phorylated and dephosphorylated MIC(IQ) show actin-activated ATPase activity; however, HCP increases

91 ed for both actin-translocating activity and actin-activated ATPase activity; however, these activiti

92 Both 546- and 625-MDE exhibited actin-activated ATPase and actin-binding activities simi

93 W546- and V413W-MDE display actin-activated ATPase and actin-binding properties simi

94 atic increase in intrinsic motor properties, actin-activated ATPase and in vitro actin gliding veloci

95 Despite similar steady-state actin-activated ATPase and unloaded in vitro motility-sl

96 ep (ADP release) in the smooth muscle myosin-actin-activated ATPase cycle that is modulated by regula

97 atalytic motor domain, and characterized its actin-activated ATPase cycle using quantitative equilibr

98 DP release is the rate-limiting step for the actin-activated ATPase cycle.

99 DP release is the rate-limiting step for the actin-activated ATPase cycle; thus, HuM5B is a high duty

100 king strokes, the MYH7b isoforms have slower actin-activated ATPase cycles and actin sliding velociti

101 She3p does not affect the maximal actin-activated ATPase in solution or the velocity of mo

102 lly decreased in vitro motility velocity and actin-activated ATPase, in contrast to other MYH7 mutati

103 In both cases, actin-activated ATPase inhibition is indicated mainly by

104 At nanomolar calcium levels, actin-activated ATPase is low and the molecule is folded

105 Steady-state actin-activated ATPase measurements revealed a V(max) of

106 Actin-activated ATPase measurements using the expressed

107 ct the Vmax, and increased the Km values for actin-activated ATPase of HMM.

108 hat is responsible for the inhibition of the actin-activated ATPase of myosin.

109 Regulation of the actin-activated ATPase of smooth muscle myosin II is kno

110 Actin-activated ATPases of SMM and XL-Rh-SMM were simila

111 sence of 14-3-3beta had little effect on the actin-activated ATPase or motile activities of Myo1c.

112 The maximum actin activated ATPase rate (V(max)) and the actin conce

113 indicate that it has a 2-fold higher maximum actin-activated ATPase rate (kcat = 1.5 +/- 0.1 s-1) and

114 , V0 is linearly correlated with the maximal actin-activated ATPase rate (vmax), which is limited by

115 t sliding velocity, whereas Loop 2 modulates actin-activated ATPase rate in Dictyostelium myosin, but

116 The maximum actin-activated ATPase rate is relatively slow (k(cat) =

117 ate of actin filament sliding or the maximal actin-activated ATPase rate of S1 or HMM constructs.

118 D515N mutation had no effect on steady-state actin-activated ATPase rate or load-dependent detachment

119 found, surprisingly, that in vitro solution actin-activated ATPase rates were increased (higher Vmax

120 tion, which increases activity of the myosin actin-activated ATPase, resulting in contraction.

121 ity and doubles myosin in vitro motility and actin-activated ATPase velocities, thereby involving a c

122 K(ATPase) of the actin-activated ATPase was 62 microm, which is much high