ライフサイエンスコーパス: adenylate kinase

1 G-actin and the open-to-closed transition of adenylate kinase.

2 state in the ligand-free form of the enzyme adenylate kinase.

3 ic contributions of dynamics to catalysis in adenylate kinase.

4 method with the conformational transition of adenylate kinase.

5 o locate and delineate an anesthetic site on adenylate kinase.

6 conserved sequence elements of p21(Ras) and adenylate kinase.

7 alkanol site so-defined spans two domains of adenylate kinase.

8 In particular, we focus on adenylate kinase.

9 action that was further enhanced by purified adenylate kinase.

10 pha-helices with overall topology similar to adenylate kinase.

11 that it folds in a manner similar to that of adenylate kinase.

12 ich is different from the situation found in adenylate kinase.

13 s to the location of the AMP-binding site in adenylate kinase.

14 this activity was purified and identified as adenylate kinase.

15 motion associated with substrate binding in adenylate kinase.

16 eport of NDP kinase activity associated with adenylate kinase.

17 ion 77, which is located in the main body of adenylate kinase.

18 ibutable to an extramitochondrial isoform of adenylate kinase.

19 adenosine-5'-pentaphosphate, an inhibitor of adenylate kinase.

20 n in the active site of the metabolic enzyme adenylate kinase.

21 fluctuations, as revealed through studies of adenylate kinase.

22 denosine back to ATP by adenosine kinase and adenylate kinase.

23 the ABC transporter CFTR can function as an adenylate kinase.

24 irradiation could then trap the (32)P on the adenylate kinase.

25 shown to share structural similarities with adenylate kinases.

26 is examined and compared with those of other adenylate kinases.

27 sequence and structural conservation in all adenylate kinases.

28 utral cholesterol ester hydrolase 1 (Nceh1), adenylate kinase 1 (Ak1), inositol polyphosphate 5-phosp

29 ucleoside triphosphate diphosphohydrolase 5/ adenylate kinase 1/cytidine monophosphate kinase 1 axis

30 her with cytidine monophosphate kinase-1 and adenylate kinase-1, constitute an ATP hydrolysis cycle t

31 s such as glutamate dehydrogenase 2 (GLUD2), adenylate kinase 2 (AK2) and transketolase (TKT).

32 rial apoptosis-inducing factor 1 (AIFM1) and adenylate kinase 2 (AK2) as gatekeeper of ATP synthase.

33 itochondrial energy metabolism and caused by adenylate kinase 2 (AK2) deficiency.

34 r dysgenesis caused by loss of mitochondrial adenylate kinase 2 (AK2) function.

35 Here we find that adenylate kinase 2 (AK2), a mitochondrial enzyme that re

36 me-wide CRISPR screen in MM cells identified adenylate kinase 2 (AK2), an enzyme critical for high-en

37 We found that AIFM1 binding to adenylate kinase 2 (AK2), an essential enzyme that maint

38 Adenylate kinase 2 (AK2), which balances adenine nucleot

39 The gene AK2 encodes the phosphotransferase adenylate kinase 2 (AK2).

40 Adenylate kinase 2 plays key roles in cellular energy an

41 ing two of high confidence, calreticulin and adenylate kinase 2.

42 sequence to follow the release of Smac, Omi, adenylate kinase-2, cytochrome c, and apoptosis-inducing

43 eous release of cytochrome c, Smac, Omi, and adenylate kinase-2.

44 nuclear-encoded purine biosynthetic enzyme, adenylate kinase 4 (AK4).

45 In this study, we show that adenylate kinase-4 (AK4) is a progression-associated gen

46 Limbic encephalitis with antibodies against adenylate kinase 5 (AK5) has been difficult to character

47 nucleoside diphosphate kinase (Ndk), ATPase, adenylate kinase, 5'-nucleotidase, and ATP-modifying enz

48 ributing to root growth control: Arabidopsis Adenylate Kinase 6 (AAK6).

49 estis tissue revealed a critical mutation in adenylate kinase 9 (AK9) that impaired splicing, leading

50 gh rudimentary, bears resemblance to that of adenylate kinase (a P-loop NTPase enzyme).

51 nderlying conformational energy landscape of adenylate kinase, a crucial protein for signal transduct

52 The adenylate kinase active center probe P(1),P(5)-di(adenos

53 hypertrophy, the reduced creatine kinase and adenylate kinase activities limited energy delivery to t

54 elevant concentrations of AMP, CFTR exhibits adenylate kinase activity (ATP + AMP &lrarr2; 2 ADP).

55 We found that CFTR also has adenylate kinase activity (ATP + AMP <=> ADP + ADP) that

56 Because CFTR NBD2 has reversible adenylate kinase activity (ATP + AMP<==> ADP + ADP) that

57 ), CFTR Cl(-) channel function is coupled to adenylate kinase activity (ATP+AMP <==> 2 ADP).

58 Deficit in adenylate kinase activity abrogated AMP signal generatio

59 lier observation that mutations that disrupt adenylate kinase activity also disrupt ADP inhibition.

60 Finding that ADP inhibits function via an adenylate kinase activity also helps explain the earlier

61 The model also includes ecto-adenylate kinase activity and feed-forward inhibition of

62 studies suggest that HCV NS4B possesses both adenylate kinase activity and nucleotide hydrolase activ

63 o and in cell-free extracts, indicating that adenylate kinase activity by Mre11/Rad50 promotes DNA-DN

64 Inhibition of adenylate kinase activity diminished both actomyosin con

65 Swelling and the release of adenylate kinase activity have been determined simultane

66 ctomyosin system, which possesses endogenous adenylate kinase activity in both compartments, substrat

67 ssays demonstrated a comparable reduction in adenylate kinase activity in oda5 flagella, and also in

68 Pase activity in the presence of ATP and 2), adenylate kinase activity in the presence of ATP plus ph

69 tenance of chromosome (SMC) protein, exhibit adenylate kinase activity in the presence of physiologic

70 es cell death as evidenced by the release of adenylate kinase activity into the cell medium, with no

71 Furthermore, the results suggest that adenylate kinase activity is important for normal CFTR c

72 A CFTR gating mechanism model based on adenylate kinase activity is proposed.

73 no biochemical data demonstrating intrinsic adenylate kinase activity of a membrane-bound ABC transp

74 o suggest that a better understanding of the adenylate kinase activity of CFTR may be of value in dev

75 ly activates the ATPase activity but not the adenylate kinase activity of Fap7, identifying Rps14 as

76 of the conserved signature motif reduces the adenylate kinase activity of Rad50 but does not reduce A

77 Whereas previous work indicated that adenylate kinase activity regulated channel opening, our

78 to the hydrolysis of NTP and NDP substrates, adenylate kinase activity was detected in purified prepa

79 ve phosphotransfer mechanisms were explored; adenylate kinase activity was unaltered, and although GA

80 te photolabeling of the AMP-binding site and adenylate kinase activity were disrupted in Q1291F CFTR.

81 re maintenance in S. cerevisiae, correlating adenylate kinase activity with in vivo functions.

82 of current was attenuated when we prevented adenylate kinase activity with P1,P5-di(adenosine-5') pe

83 apparent human ortholog of Hbr1p, assays for adenylate kinase activity, autophosphorylation, and ATPa

84 We developed a biochemical assay for adenylate kinase activity, in which the radioactive gamm

85 At physiologic nucleotide concentrations, adenylate kinase activity, rather than ATPase activity m

86 he increase of ATP in Glu(-) cells is due to adenylate kinase activity, transforming AMP into ADP whi

87 roduction, a function lost in the absence of adenylate kinase activity.

88 mal subunit with an uncommon dual ATPase and adenylate kinase activity.

89 maintenance of chromosome protein, also have adenylate kinase activity.

90 h CFTR at ATP-binding site 2 is required for adenylate kinase activity.

91 that labeled CFTR, thereby demonstrating its adenylate kinase activity.

92 rase beta chain (rpoC [mhp635]) (P = 0.003), adenylate kinase (adk [mhp208]) (P = 0.001), prolyl amin

93 lations to classify functional states, using adenylate kinase (ADK) as the main case study.

94 The enzyme adenylate kinase (ADK) features two substrate binding do

95 the closed-to-open transitions of the enzyme adenylate kinase (AdK) in its substrate-free form, we co

96 ion pathway of the phosphotransferase enzyme adenylate kinase (AdK) in the absence and presence of an

97 ssays that monitor the catalytic activity of adenylate kinase (ADK) in the equilibrium transphosphory

98 Adenylate kinase (AdK) is a phosphoryl-transfer enzyme w

99 Adenylate kinase (AdK), a phosphotransferase enzyme, pla

100 tability, and function of a selected enzyme, adenylate kinase (Adk), by monitoring changes in its enz

101 Using a large set of simulated data for adenylate kinase (Adk), calmodulin and Src kinase, we fi

102 HOBr with three well-characterized proteins [adenylate kinase (ADK), ribose binding protein, and bovi

103 S17-L14-L24-L5-S14-S8-L6-L18-S5-L30-L15-SecY-adenylate kinase (Adk)-methionine aminopeptidase (Map)-i

104 lucosamine-6-phosphate deaminase (NagB), and adenylate kinase (Adk).

105 hundreds of diverse orthologs and mutants of adenylate kinase (ADK).

106 transition between open and closed states of adenylate kinase (ADK).

107 genetic relation to bacterial and eukaryotic adenylate kinases (ADK), it was concluded that the archa

108 esidues are conserved at the active sites of adenylate kinases (Adk), suggesting that Pnk and Adk are

109 Adenylate kinases (ADKs) from four closely related metha

110 The kinetics of creatine kinase (CK) and adenylate kinase (AK) activities were monitored in intac

111 phosphate kinase (Ndk), 5' nucleotidase, and adenylate kinase (Ak) activities.

112 tivity but also a previously uncharacterized adenylate kinase (AK) activity, as it catalyzed phosphot

113 en reported that NBD2 additionally possessed adenylate kinase (AK) activity.

114 at have nucleoside diphosphate kinase (Ndk), adenylate kinase (Ak) and 5'-nucleotidase activity, the

115 e characterized the conformational change of adenylate kinase (AK) between open and closed forms by c

116 K was 30-38% identical to the members of the adenylate kinase (AK) family while EhUK was more similar

117 We report evidence that adenylate kinase (AK) from Escherichia coli can be activ

118 yeast cell integrity by using the release of adenylate kinase (AK) into culture medium as a reporter

119 Adenylate kinase (AK) is a ubiquitous enzyme that regula

120 focused on the ubiquitous phosphotransferase adenylate kinase (AK) isolated from Odinarchaeota (OdinA

121 Construction of a Thermotoga neapolitana adenylate kinase (AK) library using PERMUTE revealed tha

122 ional fluctuations in the phosphotransferase adenylate kinase (AK) throughout its active reaction cyc

123 he method employs a four-enzyme system (PDE, adenylate kinase (AK) using excess CTP instead of ATP as

124 P, indicating redistribution of flux through adenylate kinase (AK), glycolytic and guanine nucleotide

125 In adenylate kinase (AK), this involves a large-amplitude r

126 In the enzyme adenylate kinase (AK), two small domains (LID and NMP) c

127 ample is provided by the three-domain enzyme adenylate kinase (AK), which catalyzes phosphotransfer b

128 secretion was the suppression in the rate of adenylate kinase (AK)-catalyzed phosphorylation of AMP b

129 Adenylate kinase (AK)-catalyzed transfer of adenine nucl

130 scherichia coli with a temperature-sensitive adenylate kinase (AK).

131 the binding interaction of Escherichia coli adenylate kinase (AK).

132 Adenylate kinase (AK; ATP:AMP phosphotransferase, EC 2.7

133 We fused cytosolic adenylate kinase (AK1) and its isoform (AK1beta) with en

134 tional changes in the LID and NMP domains of adenylate kinase (AKE) are known to be key to ligand bin

135 the reversible conformational transition of Adenylate Kinase (AKE) between the open to the closed co

136 Adenylate kinase (AKe) from E. coli is a small, single-c

137 asured for two complexes of Escherichia coli adenylate kinase (AKe), viz., AKe.

138 Adenylate kinases (AKs) are phosphotransferases that reg

139 ed cardiomyocytes, introduction of exogenous adenylate kinase along with millimolar MgATP and AMP ind

140 Knockdown of adenylate kinase also failed to affect metformin stimula

141 induced by the ADP-generating substrates of adenylate kinase, AMP and MgATP, were indistinguishable

142 This phosphotransfer function renders adenylate kinase an important component for optimal myoc

143 Application to adenylate kinase, an allosteric enzyme composed of three

144 mong these genes, ADK1 and ADO1, encoding an adenylate kinase and an adenosine kinase, respectively,

145 ifs characteristic of adenylate kinases, and adenylate kinase and ATPase activities have been reporte

146 idating this procedure on simulated data for adenylate kinase and lactoferrin, we show how cryo-EM da

147 wns demonstrated direct interactions between adenylate kinase and several phage-coded enzymes, as wel

148 intermembrane space proteins: cytochrome c, adenylate kinase and sulfite oxidase.

149 e between the tryptophan of the F137W mutant adenylate kinase and the AEDANS-labeled Cys-77 decreased

150 ononucleotide binding proteins that includes adenylate kinase and the G-proteins.

151 he intermembranous proteins cytochrome c and adenylate kinase and the release from the matrix of sequ

152 d the corresponding amplitudes of motions in adenylate kinase and their linkage to catalytic function

153 s based on their conservation among archaeal adenylate kinases and mobility within the structures.

154 rovide specific knowledge about stability in adenylate kinases and more generally suggest that molecu

155 emonstrates the presence of 5'-nucleotidase, adenylate kinase, and a putative ATP reductase activity.

156 y phosphoryl fluxes through creatine kinase, adenylate kinase, and glycolysis in preconditioned heart

157 p exhibits sequence motifs characteristic of adenylate kinases, and adenylate kinase and ATPase activ

158 , the larger-scale motions in substrate-free adenylate kinase are not random, but preferentially foll

159 Importantly, the model identifies ecto-adenylate kinase as a key regulator of ASL ATP and propo

160 These results identify adenylate kinase as a specific component of the complex.

161 cking nucleoside diphosphate kinase, can use adenylate kinase as an alternative source of nucleoside

162 esent study identifies basal ADP content and adenylate kinase as key determinants of bioenergetics du

163 axonemal module including dynein ATPases and adenylate kinase as well as CFAP52, whose mutations caus

164 tructures have been studied in five enzymes: adenylate kinase, aspartate aminotransferase, citrate sy

165 Adenylate kinase, associated with AMP signaling, is a se

166 Adenylate kinase associates with the K(ATP) channel comp

167 systems of broad biological interest such as adenylate kinase, ATP-driven calcium pump SERCA, leucine

168 mational change pathway for Escherichia coli adenylate kinase based on two crystal structures, namely

169 enylate kinase is fairly efficient, but that adenylate kinase becomes rate-limiting for DNA synthesis

170 All three ABC adenylate kinases bind and hydrolyze ATP in the absence

171 In adenylate kinases, binding of the two ADP molecules is c

172 domain of an SMC protein in complex with the adenylate kinase bisubstrate inhibitor P(1),P(5)-di(aden

173 rally-related fragments of Bacillus subtilis adenylate kinase (BsAK) and Thermotoga neapolitana adeny

174 NBD1, human NBD1, and human NBD2 function as adenylate kinases but not as ATPases.

175 r between a mesophilic and hyperthermophilic adenylate kinase, but are strikingly similar at temperat

176 ytochrome c, as ceramides induced release of adenylate kinase, but not fumerase from isolated mitocho

177 matic TS for the phosphoryl-transfer step in adenylate kinase by quantum-mechanics/molecular-mechanic

178 ting conformational transition in the enzyme adenylate kinase, by a synergistic approach between expe

179 n Escherichia coli ndk mutant, implying that adenylate kinase can meet a demand for deoxyribonucleosi

180 Adenylate kinase catalysis accelerates the transition fr

181 distinct, yet chemically related, ATPase and adenylate kinase catalytic activities that together orch

182 low creatine kinase activity, inhibition of adenylate kinase-catalyzed phosphotransfer abolished nuc

183 In pacing-induced failing heart, adenylate kinase-catalyzed phosphotransfer increased by

184 In intact myocardium, the net adenylate kinase-catalyzed phosphotransfer rate was 10%

185 this study, we examined the contribution of adenylate kinase-catalyzed phosphotransfer to myocardial

186 ibrium constants for the creatine kinase and adenylate kinase-catalyzed reactions, allows one to esti

187 lly influence their interaction with the ABC adenylate kinase CFTR.

188 When functioning as an adenylate kinase, CFTR showed positive cooperativity for

189 rther indicate that the active center of the adenylate kinase comprises ATP-binding site 2.

190 F(0) ATP synthase or the forward reaction of adenylate kinase could not fully account for the culture

191 he corresponding monophosphate by the use of adenylate kinase, creatine phosphate, and creatine kinas

192 P signal generation and reduced the vascular adenylate kinase/creatine kinase activity ratio essentia

193 ic concentrations of ADP and AMP were added, adenylate kinase-deficient Q1291F channels opened signif

194 e previously reported free energy surface of adenylate kinase, deformations along the first mode prod

195 n ABC transporter plays an important role in adenylate kinase-dependent CFTR gating.

196 idue in CFTR, Gln-1291, selectively disrupts adenylate kinase-dependent channel gating at physiologic

197 Additional studies suggested that adenylate kinase-dependent inhibition involved phosphotr

198 tive tissues, in which AMP is generated from adenylate kinase during states of high energy demand, th

199 We now demonstrate that an ecto-adenylate kinase (ecto-AK) contributes to the metabolism

200 , suggesting the involvement of cell surface adenylate kinase, F(1)F(0) ATP synthase, and nucleoside

201 cale atomic fluctuations in hinge regions of adenylate kinase facilitate the large-scale, slower lid

202 Thus, this study provides evidence that adenylate kinase facilitates the transfer of high-energy

203 not bind to the canonical ATP site found in adenylate kinase family members.

204 okinases (PRKs) are octameric members of the adenylate kinase family of enzymes.

205 domain that is a distinctive feature of the adenylate kinase family of proteins.

206 es of the enzymes are similar to that of the adenylate kinase from archaeal Sulfolobus acidocaldarius

207 Adenylate kinase from Escherichia coli (AK(eco)) populat

208 Adenylate kinase from Escherichia coli consists of three

209 to increase stability, in silico mutants of adenylate kinase from the mesophile Bacillus subtilis we

210 osed to be important in thermal stability of adenylate kinase from the thermophile Bacillus stearothe

211 The crystal structures of adenylate kinases from the psychrophile Bacillus globisp

212 The crystal structures of adenylate kinases from the thermophile Methanococcus the

213 Adenylate kinase function is critical because a rise in

214 thin a 1-2-cM interval between D9S60 and the adenylate kinase gene (AK1).

215 Deletion of the adenylate kinase gene compromised nucleotide exchange at

216 The adenylate kinase genes (adkA) were cloned from four clos

217 Comparison of shikimate kinase to adenylate kinase has led to the identification of an ade

218 Since S.acidocaldarius adenylate kinase has the invariant Lys residue as well a

219 Assigning a signal processing role to adenylate kinase identifies a phosphorelay mechanism ess

220 e bond, we succeeded in arresting the enzyme adenylate kinase in a closed high-energy conformation th

221 It resembles adenylate kinase in having a P-loop containing core stru

222 required for outer arm assembly and anchors adenylate kinase in proximity to the arm.

223 rmational dynamics of Thermotoga neapolitana adenylate kinase in the free form (TNAK) and inhibitor-b

224 An adenylate kinase inhibitor blocks Mre11/Rad50-dependent

225 osine-5')pentaphosphate (Ap(5)A), a specific adenylate kinases inhibitor, inhibited wild-type CFTR.

226 However, little is known about how an ABC adenylate kinase interacts with ATP and AMP when both ar

227 These results indicate that adenylate kinase is a naturally occurring component of s

228 ff, and suggests that the catalytic speed of adenylate kinase is an evolutionary driver for organisma

229 cale motions observed upon ligand binding to adenylate kinase is dominated by enzyme-substrate intera

230 ation of the Lys residue in S.acidocaldarius adenylate kinase is explained.

231 tation of the missing NDP kinase function by adenylate kinase is fairly efficient, but that adenylate

232 ging revealed that a previously unidentified adenylate kinase is reduced 35-50% in oda5 flagella.

233 We also demonstrate that Adenylate kinase isoenzyme 1 (AK1) inactivates antimetab

234 the glucose transporter isoform 3 (Glut-3), adenylate kinase isoenzyme 3 (AK-3), and tissue factor,

235 Here, knock out of the major adenylate kinase isoform, AK1, disrupted the synchrony b

236 esent evidence suggesting that in the enzyme adenylate kinase large "hinge bending" motions closely r

237 we measured the conversion of ADP to AMP by adenylate kinase located in the intermembrane space.

238 age of the nail-patella locus to the ABO and adenylate kinase loci on human chromosome 9q34.

239 were prepared by covalently incorporating an adenylate kinase mutant, possessing two thiol groups, in

240 e than 100-fold by addition of ADP-consuming adenylate kinase (myokinase) to a maximal activity betwe

241 With deficient adenylate kinase, nucleoside diphosphate kinase, which s

242 Here we asked whether, by similar criteria, adenylate kinase of the host cell is also a specific com

243 Adenylate kinases participate in maintaining the homeost

244 AEW, and NaOCl treatments were identified as adenylate kinase, phosphoglycerate kinase, glyceraldehyd

245 AK1 gene deletion blunted vascular adenylate kinase phosphotransfer, compromised the contra

246 nnels to metabolic challenge is regulated by adenylate kinase phosphotransfer.

247 ctivity in both compartments, substrates for adenylate kinase promoted the rate and amplitude of acto

248 concerted action of alkaline phosphatase and adenylate kinase proved crucial for ADP/ATP generation f

249 These data indicate that the adenylate kinase reaction at NBD2 contributed to the inh

250 from three organisms catalyze the reversible adenylate kinase reaction in vitro.

251 to the conserved Q-loop glutamine during the adenylate kinase reaction.

252 wo CFTR ATP-binding sites is involved in the adenylate kinase reaction.

253 for Rad50 that incorporates both ATPase and adenylate kinase reactions as critical activities that r

254 that these enzymes catalyze both ATPase and adenylate kinase reactions.

255 Cytochrome c release was accompanied by adenylate kinase release, was not associated with mitoch

256 sequences of P-type pumps and two conserved adenylate kinase sequences that coordinate Mg2+ and/or b

257 Inspection of the known structure of adenylate kinase shows that the side chains of these res

258 ycogenolysis, coupled to creatine kinase and adenylate kinase, simulated published experiments made w

259 he second system, studied in this report, is adenylate kinase (Sp-AK), which uses 2 ADP to make ATP +

260 wo different enzymatic reactions, ATPase and adenylate kinase, that share a common ATP binding site i

261 In all cases except adenylate kinase, the backbone of residues located in an

262 In adenylate kinase, the side-chain of a residue located di

263 onsidered with earlier studies on myosin and adenylate kinase, these studies also implicate a special

264 ate kinase (BsAK) and Thermotoga neapolitana adenylate kinase (TnAK) with identical modifications at

265 TP-inhibited state is based on the action of adenylate kinase to catalyze phosphoryl transfer between

266 ghlighted in case studies from myoglobin and adenylate kinase to the ribosome and molecular motors wh

267 ying thermoadaptation of enzyme catalysis in adenylate kinase using ancestral sequence reconstruction

268 iscrepancy is due to the reverse reaction of adenylate kinase utilizing AMP.

269 ucleoside monophosphate kinases tested, only adenylate kinase was found to have NDP kinase activity.

270 ighly homologous mesophilic and thermophilic adenylate kinases, we generated a series of chimeric enz

271 Based on data from non-ABC adenylate kinases, we hypothesized that ATP and AMP mutu

272 rements of the refolding of Escherichia coli adenylate kinase were analyzed.

273 Damaging mutations in AK9, which encodes an adenylate kinase, were detected in 9.6% of iNPH patients

274 Adenylate kinase, which catalyzes the reversible ATP-dep

275 design more stable variants of a mesophilic adenylate kinase with only the sequence information of o