ライフサイエンスコーパス: affinity

1 rst brace helix of human MLKL with nanomolar affinity.

2 with the TMD is the main contributor to the affinity.

3 cytokine R production independent of TCR Ag affinity.

4 late their membrane permeability and binding affinity.

5 sts to changes in protomer concentration and affinity.

6 nlarged binding cavity that enhances binding affinity.

7 cond kinetics and nanomolar-to-submicromolar affinity.

8 y cooperatively binding oxygen with moderate affinity.

9 C-tail greatly increases Mtf1's DNA binding affinity.

10 hin a range determined by the polymerization affinity.

11 ne(N)-linked carbohydrates (glycans) impacts affinity.

12 thresholds across a 25,000-fold variation in affinity.

13 s in one dimer arm retaining high antagonist affinity.

14 ated analogs that exhibit relatively reduced affinity.

15 a considerable heterogeneity in the binding affinity.

16 PPARgamma with nanomolar potency and binding affinity.

17 found in cancers lead to reduced ASCC2-ASCC3 affinity.

18 enous inputs to specific bioavailability and affinity.

19 nobodies all bind specifically and with high affinity.

20 chains that confer higher selectivity and/or affinity.

21 required for channel inactivation with high affinity.

22 s two proline residues and enhances compound affinity.

23 butions to RNA-binding, but not DNA-binding, affinity.

24 hat we use to assess the organism's proposed affinity.

25 lent ions leads to a reversal of the binding affinity.

26 Immobilizing PBP did not affect its P(i) affinity.

27 features such as network size and TF-binding affinities.

28 analyzed their subcellular locations and DNA-affinities.

29 ly well-suited for reconstructing population affinities.

30 in-like domains and binds to Pfs47 with high affinity (17 to 32 nM).

31 nalysis of viral peptide-MHC class I binding affinity across 145 HLA-A, -B, and -C genotypes for all

32 binders to MDM2, and to a family of 3-19 nM-affinity, alpha/beta-peptide-based binders to 14-3-3.

33 -cell and bulk TCR sequencing and structural affinity analyses of cytomegalovirus-specific T cells, a

34 Based on structural and affinity analyses, we designed two cyclic peptide mimics

35 analyzed in order to interpret its taxonomic affinities and its potential significance on our underst

36 ion on Earth, especially if their biological affinities and modern analogs can be identified.

37 bodies was measured by SHG, allowing binding affinities and rates of dissociation to be obtained.

38 The relative affinities and stoichiometry of the DIX-DAX interaction

39 alpha3beta4 nicotinic acetylcholine receptor affinity and activity.

40 to MIF, selectively bind MIF with nanomolar affinity and block MIF/CXCR4 without affecting CXCL12/CX

41 This approach yielded an 8-fold increase in affinity and detailed structural information for the res

42 In the design of high-affinity and enzyme isoform-selective inhibitors, we app

43 epulsive activity is a function of its Robo3 affinity and is enhanced by ligand trimerization.

44 The binding affinity and kinetics of target engagement are fundament

45 While sequence determinants of S1 affinity and many elements of the interactions of S1 wit

46 B56, these patterns are further modulated by affinity and position of B56 binding motifs.

47 ctive warhead-were designed to leverage both affinity and reactivity.

48 Moreover, tandem affinity and RT-quantitative PCR results revealed that J

49 docking studies identified patterns driving affinity and selectivity, including limited ligand flexi

50 , good bioavailability and enhanced receptor affinity and selectivity.

51 undergo isomerization that would weaken the affinity and slow polymerization.

52 e selected aptamers often exhibit suboptimal affinity and specificity.

53 It has high affinity and stability and is suitable for preclinical r

54 gonists and inverse agonists displaying high affinity and subtype selectivity over the antitarget M(2

55 n for optimal selectivity and high substrate affinity and transport rates.

56 plexes is complicated by their typically low affinity and variable binding of the SIMs in parallel an

57 l modules with drastically different binding affinities, and with distinct and mutually exclusive bin

58 it coagulation, binds Tau with low nanomolar affinity, and inhibits cellular Tau aggregate propagatio

59 shows that serotonin adsorbs with millimolar affinity, and neutron reflectometry shows that it penetr

60 cells, bind to R-spondins (RSPOs) with high affinity, and potentiate Wnt-beta-catenin signaling, pre

61 Agonist potency, binding affinity, and relevant pharmacological parameters of 15

62 that ERFE binds BMP6 directly and with high affinity, and that antibodies targeting the N-terminal d

63 sorder in H2 and its contribution to binding affinity, and that sequence variations in H2 that do not

64 erties of biological stability, high binding affinity, and thermal stability make TNA aptamers a powe

65 r multiple enrichment rounds to harvest high-affinity aptamers as in conventional SELEX.

66 The compounds had millimolar affinities as determined by (15)N HSQC NMR.

67 CCL2 scavenging results aligned with binding affinity assays, with ACKR2-V41A cells scavenging CCL2 w

68 hosphorylated Annexin A2 (pANXA2), with high affinity at high levels of calcium.

69 nist 2 was synthesized and studied for their affinity at M(1)-M(5) mAChRs.

70 d in Mitragyna speciosa (kratom), had higher affinity at opioid receptors than at adrenergic receptor

71 we show that it is possible to tune the O(2) affinity at the open metal sites of MOFs for application

72 tigate viral genome replication, RNA-binding affinity, ATP hydrolysis activity, and helicase-mediated

73 onses, with preferential recruitment of high-affinity B cells.

74 monstrate that reconstructions of population affinity based on many traits are not necessarily more r

75 c lipid enrichment that enhances the overall affinity between the membrane and RBD-CRD.

76 The strong affinity between the metallic lithium and lithium tin al

77 nteractions are often conducted only on high affinity binders due to conservative data thresholding.

78 Our results support the relevance of high-affinity binding of ARF transcription factors to uniquel

79 High-affinity binding of InsP(8) to the XPR1 N-terminus (K (d

80 increased resistance, probably owing to high-affinity binding of SAP2 to pyrethroid insecticides.

81 strand alignment in the active site for high-affinity binding of the initiating nucleotides.

82 g the fraction of channels presenting a high-affinity binding site for PF-05089771 and suggesting tha

83 l oncogene MITF as a model, we show that low-affinity binding sites act as a competitive reservoir in

84 cuous exchange among myriads of intermediate affinity binding sites.

85 rmational changes in PCSK9 required for high-affinity binding to LDL particles.

86 hetic auxin derivative 5-Ad-IAA and its high-affinity-binding partner OsTIR1F74A.

87 ctrochemical detection by fabricating a high-affinity bispecific AEC (bsAEC) using two Catcher/Tag sy

88 biological processes because it builds high-affinity bonds by using several weak binding interaction

89 ain of eIF4GI increases not only the binding affinity but also the selectivity among these mRNAs.

90 These compounds display high binding affinity, but have poor physicochemical properties and a

91 nd low-affinity populations that differed in affinity by 100-fold.

92 A second-generation series improved on affinity by incorporating known reversible inhibitor pha

93 lethyl) moiety particularly enhanced mA(3)AR affinity by polar interactions with the extracellular lo

94 We therefore expressed a low affinity Ca(2+) indicator (ER-GCaMP6-150) in the ER, and

95 e-of-the-art small-molecule relative binding affinity calculations, which have been shown to be capab

96 Here, we show that affinity can be significantly improved by enriching the

97 c point mutations that decrease drug binding affinity can produce TKI resistance, and second- and thi

98 ch combines commercially available protein A affinity capture, targeted analyte isolation by 2D-LC, a

99 Following affinity capture, we generated calibration curves (five-

100 type allele by measuring its ligand binding affinity, CCL2 scavenging efficiency, and cell activatio

101 by multiple Ags, we coencapsulated the high-affinity CD4(+) mimotope (BDC2.5(mim)) of islet autoanti

102 urified from seabuckthorn seeds using chitin-affinity chromatography that showed antifreeze property

103 phosphopeptide enrichment materials based on affinity chromatography to either immobilized metals (IM

104 amma-AApeptides reveal much-enhanced binding affinity compared to the p53 peptide.

105 s from its lower single-stranded DNA (ssDNA) affinity, compared to that of ScRad51.

106 Furthermore, of the higher-affinity compounds examined, none showed any agonist act

107 n provides a structural basis for their high-affinity, covalent interactions.

108 For low-affinity decoys, noise in the level of unbound TF always

109 In contrast, for high-affinity decoys, noise levels first increase with increa

110 eIF4E-transporter, and RPS2 showed >10-fold affinity differences.

111 pidly activated cognate B cells, with little affinity discrimination.

112 r application in PCR-based diagnostics, high-affinity DNA aptamer generation, site-specific labeling

113 T cell Ag recognition, in which Ag dose and affinity do not provide any additional response-specific

114 Label-free affinity electrochemical biosensors constructed with sem

115 The first achieves pHLA recognition via affinity-enhanced versions of natural TCRs (e.g., immune

116 ethod, but also reveal drastically different affinity enhancing mechanisms between two closely relate

117 oTag or the SNAP-tag, permitting a multistep affinity enrichment of specific protein complexes.

118 Resting affinities for PIP(2) differ for Kv7.4 and Kv7.5 based o

119 eed can convert S7P to SBP, and have similar affinities for S7P and the canonical substrate fructose

120 cate that this diversity is defined by their affinities for specific co-chaperones such as HSPH2.

121 Their affinities for the A(2A) AR were experimentally determin

122 pophilic drugs, such as taxanes, have a high affinity for adipose tissue and a resulting higher volum

123 loping memory and GC B cells differ in their affinity for antigen throughout the immune response.

124 B cell precursor frequency, B cell receptor affinity for antigen, antigen avidity, and other factors

125 ons and physicochemical treatments, a higher affinity for bacteria than simulated intestinal epitheli

126 e is a potent analgetic agent with very high affinity for brain nicotinic acetylcholine receptors (nA

127 on rate and, paradoxically, improves binding affinity for carbapenems.

128 Neutral diclofenac displayed greater affinity for DMPC bilayers than anionic diclofenac.

129 ealed that Mtf1 with an intact C-tail has no affinity for DNA but deletion of the C-tail greatly incr

130 Dendritic galactose moieties have a high affinity for galectin-expressing tumor cells.

131 (2+)-release assays; its potency and binding affinity for Ins(1,4,5)P(3)R were similar to those of ad

132 We generated an IL-10 mutant with enhanced affinity for its IL-10Rbeta receptor using yeast surface

133 CS is to overcome 4 to 6 log-folds stronger affinity for its natural inhibitor, serine acetyltransfe

134 defeating a sizable ligand with subnanomolar affinity for its receptor, designing antagonists with an

135 xenon than for krypton, and have comparable affinity for Kr and N(2).

136 tudy show that (i) mimosine has high binding affinity for metallic cations at alkaline pH, Fe(III)-mi

137 C01-class, CD4bs-directed bNAbs and dampened affinity for nonneutralizing Abs.

138 mutation in NLGN4 did not affect its binding affinity for NRXNs or its capacity to form homodimers.

139 To identify such compounds with binding affinity for polyQ aggregates, we embarked on systematic

140 here was selection against B cells with high affinity for self-antigen.

141 cetyltransferase (SAT), as compared with its affinity for substrate, OAS.

142 is due to altered nsDNA binding kinetics and affinity for the bacterial chromosome.

143 ide analogue M4K2149 with reduced off-target affinity for the ion channel.

144 QTL that significantly decreases the binding affinity for the repressor Broad, driving differential a

145 possess improved antigenicity with retained affinity for VRC01-class, CD4bs-directed bNAbs and dampe

146 -organic frameworks are found to have higher affinity for xenon than for krypton, and have comparable

147 isms that govern the differentiation of high-affinity germinal center (GC) B cells into memory B cell

148 Yet despite the promising advances of affinity grids over the past decades, no single strategy

149 High-affinity guests have been reported for the macrocyclic h

150 a specific, stable, and abundant target for affinity-guided delivery of anticancer drugs.

151 We studied humans with high affinity haemoglobin and compensatory polycythaemia.

152 omeostasis through its recruitment to a high-affinity heterotrimeric receptor complex (IL-2Ralpha/IL-

153 the respective H(2)Rs with moderate to high affinity (human/guinea pig/mouse K(d): 24/28/94 nM).

154 We utilized mass spectrometry enhanced by affinity hydrogel particles (analytical sensitivity = 2.

155 ng the cellular mechanisms that lead to high-affinity IgE production is required to develop better th

156 Here we show that IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated

157 hC3Nb3 nanobody binds C3 with low nanomolar affinity in an SDS-resistant complex, and the nanobody i

158 es are thus required to maximise the binding affinity in host-guest complexes.

159 tors of methyltransferase enzymes to show an affinity in the nanomolar range.

160 inding, with an 80-fold increase in hepcidin affinity in the presence of iron.

161 ) for full effect and increasing the binding affinity increases efficacy.

162 ranches in the tree are likely to harbor key affinity-increasing mutations?

163 ing advantage of the well-described and high-affinity interaction between KRAS and CRAF, and we provi

164 ency in HEK293T cells, implying that a lower-affinity interaction of the SNAP25 zDABM with zDHHC17 is

165 lated by the UBL domain, which mediates high-affinity interaction with the polyubiquitin chain.

166 that the dependence is solely on the binding affinity, irrespective of pH, IS, and resin ligand densi

167 Remarkably, the relative affinity is further increased to 60-fold by dsDNA damage

168 we find that the predicted absolute binding affinity is predictive of immunogenicity.

169 This change in affinity is then translated effectively into control of

170 Moreover, the electron affinity is used as a simple descriptor to make a predic

171 The approach enables affinity isolation and downstream analyses without induc

172 We developed variants with varying binding affinities (K(D) as low as 216 pM), co-crystallized it w

173 compounds that bind TAR with low micromolar affinity (K(D) values ranging from 3.6 to 22 mum).

174 that RPA binds RNA with an unexpectedly high affinity (K(D) ~ 100 pm).

175 rs for Signal Amplification (gamma), Binding affinity (K(d)), Receptor activation Efficacy (epsilon),

176 d acceptor subsites showed similar substrate affinity (Km) values to the wildtype (WT) Km value but h

177 size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence

178 The gain in affinity led to almost equal inhibition of wildtype PfFN

179 , and in experiments comparing low- and high-affinity ligands and oncogenic EGFR mutants.

180 radiolabeled mono- (scFv) and bivalent (mAb) affinity ligands specific for the endothelial cell adhes

181 ty of VSDs by dynamic encapsulation and high-affinity ligands to target the construct to specific neu

182 ed carboxylate coated magnetic beads without affinity ligands.

183 antibody (mAb) epitopes while retaining high-affinity mAb epitopes.

184 ing, but sequence degeneracy and low binding affinities make them difficult to identify.

185 Affinity maturation and terminal differentiation of B-ce

186 Affinity maturation continued for 6 to 9 mo following va

187 -) mouse B cells also cannot complete CSR or affinity maturation despite accumulating significant mut

188 These findings provide insight into the affinity maturation process and potential cross-reactivi

189 r time for some individuals, consistent with affinity maturation.

190 gh a Darwinian evolutionary process known as affinity maturation.

191 tensin II infusion in mice, we found that an affinity matured nanobody antagonist has comparable anti

192 o striking structural changes for parent and affinity-matured antibodies bound to ARG2, with a large

193 The affinity-matured rabbit mAb was then humanized by comple

194 gen (O(2)) status of the tumor and HBOC O(2) affinity may play a role in increased O(2) delivery to t

195 therapeutic agents that combine an optimized affinity-modifying group and optimized albumin binders t

196 l molecule binding, measuring ligand-binding affinity, monitoring protein folding and unfolding and d

197 copy number can reduce the abundance of low-affinity monoclonal antibody (mAb) epitopes while retain

198 CoV-2 spike (S) glycoprotein exhibits a high-affinity motif for binding TCRs, and may form a ternary

199 ngs demonstrated the effectiveness of P-Tris affinity nanofiber membrane for the recovery of lysozyme

200 r syndromic forms of IGD and maps to a TCF12 affinity network.

201 Higher-affinity next-generation ROS1 TKIs developed to have imp

202 vivo, and suggest that differential binding affinities of ARF subfamilies underlie diversity in cis-

203 y of glycans in their purified form, the low affinities of GBP-glycan interactions, and limitations i

204 scently labeled library, allows the relative affinities of glycan ligands to be ranked.

205 r tool to determine hH(3,4)Rs/mH(4)R binding affinities of ligands binding to these receptors.

206 ndages of L. illecebrosa also strengthen the affinities of megacheirans as stem-group chelicerates, i

207 6) as a probe for competitively defining the affinities of N-terminal SC(1-246) variants preselected

208 (HSM), capable of accurately predicting the affinities of PBD-peptide interactions across multiple p

209 force calculations to determine the relative affinities of PLB for candidate sites on SERCA.

210 d to the development of InhA inhibitors with affinities of up to 250 nM.

211 Next, we show that the affinity of a single molecule of profilin to the polypro

212 emonstrate a strategy to improve the binding affinity of aptamers by modifying their sequences upon t

213 quaternary ammonium group imparted a strong affinity of cationic perfluorooctaneamide ammonium compo

214 binding is also induced by the differential affinity of crystal facets to water molecules in the fir

215 and quantified interaction kinetics and the affinity of HuNoV virus-like particles (VLPs) to lipid v

216 vative, showed increased beta-lactam binding affinity of PBP5 of HOU503 compared with that of R497.

217 red 3D-structures indicates no difference in affinity of TDF binding by RT encoded by intra-host HBV

218 of the TCR gamma chain and modulated by the affinity of the CDR3 region of the TCRdelta chain, which

219 l sfRNA formation by maintaining the binding affinity of the corresponding xrRNAs to Xrn1.

220 grative studies reveal an exceptionally high affinity of the EhActn2 CaMD for Ca(2+), binding of whic

221 that could potentially enhance the membrane affinity of the entire RBD-CRD construct.

222 gation is efficient, does not compromise the affinity of the parental antibody, and utilizes chemical

223 We then determine the binding affinity of Wnt3 to its receptor, Frizzled1 (Fzd1), usin

224 We measured in situ two-dimensional affinity on individual live T cells from thymus, spleen,

225 y] into their two enantiomers, isolating the affinity on the corresponding (S)-eutomers (K(i) = 6.30

226 The model displays a threshold in affinity parameters below which there is an appreciable

227 in geometry for strong binders than for low-affinity peptides.

228 2;1 functions as a chloroplast-localized low-affinity Pi transporter that mediates UV tolerance and r

229 phore with negative charge showed high M(2)R affinities (pK(i) (radioligand competition binding): 9.1

230 ion of a common BMP signal, conferred by low affinity pMad/Medea binding motifs, can contribute to th

231 stages comprising two distinct high- and low-affinity populations that differed in affinity by 100-fo

232 that exploits aptamers as minimally invasive affinity probes.

233 Here, we use nucleosome affinity proteomics with a library of nucleosomes that d

234 Here we describe serial capture affinity purification (SCAP), in which two separate prot

235 Here, we employed translating ribosome affinity purification (TRAP) and RNA sequencing, TRAP-se

236 Affinity purification and pairwise yeast two-hybrid anal

237 Cell type-specific translating ribosome affinity purification and qPCR was used to compare mRNA

238 pplied a combination of translating ribosome affinity purification and ribosome profiling to identify

239 s biotin ligases in comparison with one-step affinity purification approaches.

240 Reciprocal dual-affinity purification of endogenous USP13 followed by ma

241 Our approach combines serial affinity purification, live cell imaging, and cross-link

242 In this study, quantitative affinity purification-mass spectrometry enabled the deli

243 broblasts undergoing LTbetaR stimulation and affinity-purification mass spectrometry for the LTbetaR

244 Of particular concern was the high-affinity R-state PolyhHb, which led to no improvement in

245 n clinical isolates for quinolones from drug affinity (R2>0.9).

246 Our studies support a facile method for affinity-ranking hits.

247 IL-2Ralpha, in part, comprises the high affinity receptor for IL-2, a cytokine important in immu

248 pomycin receptor kinase A (TrkA) is the high-affinity receptor for nerve growth factor.

249 tissues bound to resident cells via its high-affinity receptor, FcepsilonRI.

250 In vivo-specific deletion of the Sema3E high-affinity receptor, plexinD1, on macrophages led to the i

251 profilin is negatively cooperative, with the affinity reduced ~11-fold.

252 ight bromodomains of the family with similar affinity, resulting in a diverse range of biological eff

253 We present revised SH2-pTyr affinity results and propose a new analysis pipeline for

254 y, SERT inhibitor Lu AF60097, the first high-affinity S2-ligand reported and characterized here, allo

255 antitating the phage clones before and after affinity selection and aligning the sequences of the mos

256 ivative SH53d-acid shows superior (>40-fold) affinity selectivity and is a positive modulator.

257 the GM1 ganglioside pentasaccharide yielded affinities similar, within a factor of 2, to those of na

258 RBD, leading to the identification of a high-affinity single-chain fragment variable region (scFv).

259 T6 C-terminus promotes binding at the higher affinity site through recognition of nucleosomal DNA.

260 Unlike the other high-affinity sites, which bind NC with exothermic energetics

261 t in biological communities in favor of warm-affinity species (i.e., thermophilization).

262 substantially, but not in step with binding affinity, suggesting distinct modes of interactions in t

263 s, we found that the immunodominance of high-affinity T cell clones declined during the chronic infec

264 of tags, such as NMR tags, fluorescent tags, affinity tags, and alkyne tags, to proteins.

265 ta2-beta3 loop sequences present in two high-affinity TBPs (K(D) values of 4.2 +/- 0.3 and 3.0 +/- 0.

266 ion, (Sc)CRM1 exhibits 16-fold lower binding affinity than (Hs)CRM1 toward PKI-NES and significant di

267 lular lectin domain binds to ATP with higher affinity than P2K1 (dissociation constant [K (d)] = 44.4

268 ly residual FcgammaRI binding with differing affinities that are either complement competent or silen

269 uman Immunoglobulin G antibodies with target affinities that are superior to candidates derived from

270 -a noncooperative homodimer with high oxygen affinity that existed before the gene duplication that g

271 hiol-rich reducing molecules with high Cu(I) affinity, they are potential competitors for a copper io

272 tic states, transcription factor-DNA binding affinity thresholds and influences of given transcriptio

273 directly with one of these lncRNAs with high affinity through its HMG DNA-binding domain in vitro.

274 o fully melted iron meteorites with isotopic affinities to carbonaceous chondrites.

275 titatively predicts residue-specific binding affinities to nanoparticles and it expresses binding coo

276 eties that are known to exhibit high binding affinities to serum albumin.

277 bfractions of asphaltenes having contrasting affinities to the water-oil interface are used as surfac

278 C2A domain binds with low nanomolar apparent affinity to PIP(2) in lipid vesicles that also contain b

279 To assess their affinity to PIPs we have developed a new cell free assay

280 importantly, a significantly higher antibody affinity to RSV G was observed in nasal washes from earl

281 individual CRYs, with CRY1 exhibiting higher affinity to the BMAL1/CLOCK complex than CRY2.

282 selective for STAT3 and bind with nanomolar affinity to the N-terminal and coiled-coil domains.

283 molecule, (+)SW033291, that binds with high affinity to the PGE2-degrading enzyme 15-hydroxyprostagl

284 specific binding and to increase the binding affinity to the receptor.

285 Modified PS ASOs display better binding affinity to the target RNAs and increased binding to pro

286 oward PKI-NES and significant differences in affinities toward potential CRM1 inhibitors.

287 nding on the AuNP-MIP sensor revealed a high affinity toward glucose with a dissociation constant (K(

288 an-containing RGD peptides display increased affinity toward integrin alpha(v)beta(3) and enhanced se

289 dies; lead optimization of aggregate-binding affinity, unbound fractions in brain, permeability, and

290 ensitization, we enhanced nicotinic receptor affinity using NS9283 stimulation at the unorthodox alph

291 show that high-affinity vaccines targeting rare B cells capable of broa

292 The affinity values of the three spicy substances with hTRPV

293 The highest-affinity variant contained seven amino acid changes and

294 only quantify the GNP-glycan-lectin binding affinities via a new fluorescence quenching method, but

295 The improved binding affinity was attributable primarily to a reduced rate of

296 II isoforms in the nanomolar range, while no affinity was encountered for off-targets, such as hCA I

297 Given its high specificity and affinity, we anticipate that these anti-CoV-2 antibodies

298 rrelate with ionic strength (IS) and binding affinity, we show that the dependence is solely on the b

299 phology and ultrastructure reject cycadalean affinities, whereas its guard cell shape and stomatal le

300 omolar cellular potency and nanomolar target affinity with balanced physicochemical properties and lo