ライフサイエンスコーパス: affinity chromatography

1 chia coli and then purified by metal chelate affinity chromatography.

2 erexpressed protein by immobilized metal ion affinity chromatography.

3 otein (CabP) in S. pneumoniae using c-di-AMP affinity chromatography.

4 was purified by nickel-nitrilotriacetic acid affinity chromatography.

5 cells and purified by immobilized metal ion affinity chromatography.

6 The enzyme was purified using cobalt ion affinity chromatography.

7 ed immunoprecipitation and immobilized metal affinity chromatography.

8 lated from crude cell membrane fractions via affinity chromatography.

9 he detergent Fos-choline-12, and purified by affinity chromatography.

10 teases can be exploited for highly selective affinity chromatography.

11 coli and purified to homogeneity by means of affinity chromatography.

12 per motif transcription factor, by G-box DNA affinity chromatography.

13 ied by nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography.

14 f immobilized metal affinity and metal oxide affinity chromatography.

15 m a variety of species and tissue sources by affinity chromatography.

16 binant His(6)-tagged TcNr was purified by Ni affinity chromatography.

17 and easily purified from Escherichia coli by affinity chromatography.

18 glycated species can be enriched by boronate affinity chromatography.

19 tag, and the protein was purified by nickel affinity chromatography.

20 in surface labeling followed by streptavidin-affinity chromatography.

21 antibodies were removed from immune sera by affinity chromatography.

22 and mTORC2 from HEK-293 cells using FLAG-M2 affinity chromatography.

23 Neurexin1 as a receptor for LRRTM2 based on affinity chromatography.

24 endothelial Ag, in this study purified by Ab-affinity chromatography.

25 nt PrtP copurifies with PrcB-6xHis in nickel affinity chromatography.

26 purified from a bacterial host using nickel affinity chromatography.

27 chromatography and immobilized bTf antibody affinity chromatography.

28 s purified from human placental membranes by affinity chromatography.

29 rs and CUG-BP1 to the exonic silencer by RNA affinity chromatography.

30 lene glycol precipitation followed by lectin affinity chromatography.

31 g to CCPs 7-8 and 19-20 bind well in heparin-affinity chromatography.

32 lot and ELISA, and OPN was purified using Ni affinity chromatography.

33 in E. coli, and purified with Ni-NTA agarose affinity chromatography.

34 teins to aid in their purification via metal affinity chromatography.

35 rotein could be purified in a single step by affinity chromatography.

36 (DE3) and purified the enzyme by cobalt ion affinity chromatography.

37 ich might have been co-purified during metal affinity chromatography.

38 sed recombinantly in E. coli and purified by affinity chromatography.

39 estimates of dissociation constants and FcRn affinity chromatography.

40 uent pulldown experiments with biotin-avidin affinity chromatography.

41 re exploited to purify the native protein by affinity chromatography.

42 red by surface plasmon resonance and heparin affinity chromatography.

43 and purified by nickel-nitrilotriacetic acid affinity chromatography.

44 nium sulfate precipitation, ion exchange and affinity chromatography.

45 e, A1AT (Zemaira) or M-type A1AT purified by affinity chromatography.

46 sed in a heterologous system and purified by affinity chromatography.

47 ividual conformers could be isolated by bNAb affinity chromatography.

48 xpressed in Escherichia coli and purified by affinity chromatography.

49 mplex purified under anaerobic conditions by affinity chromatography.

50 PSF) as an interacting protein with Fox-3 by affinity-chromatography.

51 que that couples isotachophoresis (ITP) with affinity chromatography (AC) to achieve rapid, selective

52 dy of coupling of isotachophoresis (ITP) and affinity chromatography (AC) to effect rapid, selective

53 extract or a plant total protein extract by affinity chromatography against smGN.

54 Using glutathione-S-transferase affinity chromatography, AgmU was found to interact eith

55 cific IgGs from four representative sera via affinity chromatography, allowing us to determine the co

56 The 10.1.1 Ab-affinity chromatography also purified LFA-1, an integrin

57 ication as well as tandem size exclusion and affinity chromatography analyses provided the first bioc

58 specifically interacts with DnaA protein by affinity chromatography and a solid phase binding assay,

59 nrich the modified peptides by avidin-biotin affinity chromatography and analyze them by nanoRP-UPLC-

60 ng Fab/Rev complex was purified by metal ion affinity chromatography and characterized by analytical

61 rs, secreted into culture media, purified by affinity chromatography and characterized by biolayer in

62 n, as demonstrated by 1) binding assays (DNA affinity chromatography and ChIP) showing specific IGF-I

63 DNA affinity chromatography and chromatin immunoprecipitatio

64 associate with human DNA polymerase delta by affinity chromatography and coimmunoprecipitation assays

65 nteraction assays of recombinant kinases and affinity chromatography and competition affinity assays.

66 Affinity chromatography and cross-linking experiments sh

67 To study resin fouling in situ, we coupled affinity chromatography and Fourier transform infrared (

68 essed in E. coli, purified by Ni-NTA agarose affinity chromatography and functionally characterized i

69 y by anion exchange chromatography, His-Trap affinity chromatography and gel filtration.

70 Moreover, using affinity chromatography and gel shift assays, we demonst

71 Here, employing affinity chromatography and glycerol gradient centrifuga

72 e-catecholic adducts, that were separated by affinity chromatography and high performance liquid chro

73 he crosslinking activity was purified by RNA affinity chromatography and identified as nucleolin by m

74 By using phosphoprotein affinity chromatography and immunoblot analyses, we show

75 Heparin affinity chromatography and intrinsic fluorescence kinet

76 To address this question, we used affinity chromatography and isoelectric focusing to frac

77 enient purification by immobilized metal-ion affinity chromatography and labeling with [(99m)Tc(CO)3]

78 hese common alleles, we used oligonucleotide affinity chromatography and liquid chromatography-mass s

79 Here, using affinity chromatography and mass spectrometry experiment

80 DNA affinity chromatography and mass spectrometry identified

81 Affinity chromatography and mass spectrometry identified

82 We used affinity chromatography and mass spectrometry to discove

83 Using affinity chromatography and mass spectrometry we identif

84 Using affinity chromatography and mass spectrometry we reveale

85 Using RNA-affinity chromatography and mass spectrometry, we furthe

86 By combining siRNA affinity chromatography and mass spectrometry, we have i

87 Using affinity chromatography and mass spectrometry, we identi

88 Using affinity chromatography and mass spectrometry, we identi

89 Using affinity chromatography and mass spectrometry, we identi

90 Here, employing affinity chromatography and mass spectrometry, we identi

91 Affinity chromatography and proteomics analysis using KS

92 Using a combination of immunohistochemistry, affinity chromatography and proteomics, we identified th

93 in human plasma were confirmed by employing affinity chromatography and proteomics.

94 c binding proteins were identified by Zn(2+)-affinity chromatography and proteomics.

95 ralogs, whose composition we corroborated by affinity chromatography and proximity labeling mass spec

96 Using affinity chromatography and split-Yellow Fluorescent Pro

97 -enriched fractions, using immobilized metal affinity chromatography and tandem mass spectrometry.

98 Immobilized metal affinity chromatography and titanium dioxide chromatogra

99 Yersinia pseudotuberculosis were purified by affinity chromatography and used as immunogens to determ

100 3 proteins were not detected in the RNP, but affinity chromatography and yeast two-hybrid experiments

101 r derivatized to IMAC (immobilized metal ion affinity chromatography) and is investigated by loading

102 hia coli, purified in a one-step fashion via affinity chromatography, and activity as well as specifi

103 e enzyme-linked immunosorbent assay (ELISA), affinity chromatography, and bio-layer interferometry (B

104 expression, purified using immobilized metal affinity chromatography, and characterized for T cell bi

105 a pastoris, purified the proteins via tandem affinity chromatography, and determined MDR3-specific AT

106 ecipitation and nickel-nitrilotriacetic acid affinity chromatography, and further supported by co-loc

107 bsence of denaturation and fixation, heparin-affinity chromatography, and high-resolution LC-MS/MS, a

108 munoaffinity purification, immobilized metal affinity chromatography, and nanoflow liquid chromatogra

109 vels in Escherichia coli, purified by nickel affinity chromatography, and partially characterized.

110 tagenesis, coupled with splicing assays, RNA-affinity chromatography, and protein overexpression, and

111 erum IgGs were subjected to standard antigen-affinity chromatography, and resulting elution, wash, an

112 riched by detergent phase separation, lectin affinity chromatography, and SDS-PAGE.

113 CPO was purified by affinity chromatography, and the purified enzyme was abl

114 purified from seeds, were separated by metal affinity chromatography, and their properties (GTPase ac

115 s like enzyme-linked immunosorbent assay and affinity chromatography are commercially available for t

116 native gel electrophoresis, traditional and affinity chromatography assays and surface plasmon reson

117 When AUG3 was enriched from seedlings by affinity chromatography, AUG1 was detected by immunoblot

118 ly(dimethylsiloxane) (PDMS), implements cell-affinity chromatography based on the selective cell-capt

119 Two fractions of transferrin, separated by affinity chromatography based on their binding or not to

120 arose bead-based microfluidic device for the affinity chromatography-based capture and detection of R

121 A simple, cheap, and highly reproducible affinity chromatography-based method has been developed

122 Here, using affinity chromatography, bioinformatics analyses, NAD sy

123 In addition, MAb affinity chromatography can be expensive, the necessary

124 emonstrated for the first time how anti-IgG4 affinity chromatography can be used to prepare physiolog

125 easured using three primary assays (boronate-affinity chromatography, capillary isoelectric focusing

126 atographic phase to create cellular membrane affinity chromatography (CMAC) columns, CMAC(1321N1) and

127 ationary phase to form two cellular membrane affinity chromatography (CMAC) columns, CMAC(hOCT1(G465R

128 ey specifically interact, as demonstrated by affinity chromatography, co-immunoprecipitation, biosens

129 Using affinity chromatography, coimmunoprecipitation, retaggin

130 irst time, the implementation of a Protein-A affinity chromatography column as the first dimension ((

131 i-CD14, or anti-CD19 antibodies were used as affinity chromatography columns to separate target blood

132 gh reconditioning power of protein G'e-based affinity chromatography columns.

133 ins from human U2OS osteosarcoma cells using affinity chromatography coupled to mass spectrometry emp

134 Using affinity chromatography coupled to multidimensional prot

135 we describe the use of peptide aptamer-based affinity chromatography coupled with mass spectrometry t

136 Using ligand-affinity chromatography coupled with mass spectrometry,

137 Toward this goal, we used affinity chromatography coupled with proteomics technolo

138 tor (NF) 90 and NF45, as demonstrated by DNA affinity chromatography coupled with tandem mass spectro

139 With the detergent assisted lectin affinity chromatography (DALAC), a total of 1491 protein

140 xample, to fluorescent tags or 'handles' for affinity chromatography--directly on complex molecular s

141 ifications of carbon; potential uses include affinity chromatography, DNA sequencing technologies, bi

142 ied a set of V-genes heavily enriched in the affinity chromatography elution, constituting the serum

143 Tryptic phosphopeptides (immobilized metal affinity chromatography-enriched) were identified and qu

144 Affinity chromatography experiments identified the prima

145 We used affinity chromatography experiments to uncover specific

146 Finally, affinity chromatography experiments using peptides corre

147 Affinity-chromatography experiments revealed that the ly

148 d by gel-shift assay and affinity by frontal affinity chromatography (FAC).

149 2D) analytical system consisting of boronate-affinity chromatography followed by CIEF.

150 Affinity chromatography followed by mass spectrometry an

151 ing partners for CaBP5 were identified using affinity chromatography followed by mass spectrometry an

152 d a comprehensive set of Drosophila Rabs for affinity chromatography followed by mass spectrometry to

153 ocedure that combines membrane flotation and affinity chromatography for the purification of autophag

154 igned a metal-directed immobilized metal ion affinity chromatography for the sequential enrichment of

155 '-UTR) binding proteins were purified by RNA affinity chromatography from cytosolic fractions of TNF-

156 The recombinant human Hsp72 was purified by affinity chromatography from insect cells, and purity wa

157 een isolated by origin sequence-specific DNA affinity chromatography from total chloroplast proteins.

158 (v)1.2 channels by CaBP5 were analyzed using affinity chromatography, gel overlay assays, and patch-c

159 Using affinity chromatography, HCR/F bound native synaptic ves

160 system, named High Performance Multi-Lectin Affinity Chromatography (HP-MLAC), is composed of a seri

161 e rates involves the use of high-performance affinity chromatography (HPAC) and estimates of band-bro

162 ons with these particles by high-performance affinity chromatography (HPAC).

163 cow's milk (n=25) by a previously validated affinity chromatography-HPLC method.

164 FA was also quantified by an affinity chromatography-HPLC method.

165 Affinity chromatography identified 74 ZMP-binding protei

166 Ligands were purified by size exclusion and affinity chromatography, identified by proteomic mass sp

167 ichment by TiO2 and Ti(4+)-immobilized metal affinity chromatography (IMAC) by a single operator.

168 t as has been reported for immobilized metal affinity chromatography (IMAC) columns.

169 C) employing gallium-based immobilized metal affinity chromatography (IMAC) in conjunction with titan

170 etry-compatible, iron(III)-immobilized metal affinity chromatography (IMAC) methods that use either n

171 ng phosphopeptides include immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO2

172 through complexation with immobilized metal affinity chromatography (IMAC) resin.

173 e sequential Ga(3+)-Fe(3+)-immobilized metal affinity chromatography (IMAC) strategy displayed a 1.5-

174 Second, immobilized metal affinity chromatography (IMAC) takes advantage of phosph

175 Immobilized metal-ion affinity chromatography (IMAC) used to purify recombinan

176 On the other hand, immobilized metal affinity chromatography (IMAC) was used to quantify thes

177 Immobilized metal ion affinity chromatography (IMAC) with nickel-NTA was used

178 hosphopeptides through immobilized metal ion affinity chromatography (IMAC), followed by enrichment o

179 ied in a single step using Immobilized Metal Affinity Chromatography (IMAC), in-solution trypsin dige

180 5-/- embryos, using an Immobilized Metal-Ion Affinity Chromatography (IMAC), which specifically binds

181 TRAIL was accomplished by immobilized metal affinity chromatography (IMAC), which was followed by tr

182 ptides were isolated using immobilized metal affinity chromatography (IMAC).

183 ed by protective antibodies, we selected, by affinity chromatography, immunoglobulins against immobil

184 Affinity chromatography, immunoprecipitation, enzyme-lin

185 Analysis by lectin-affinity chromatography in conjunction with gas chromato

186 Using affinity chromatography in conjunction with tandem mass

187 +/- 0.4 muM) and close to that determined by affinity chromatography in the literature (6 +/- 3 muM).

188 n, we developed an approach that uses lectin affinity chromatography, ion-exchange chromatography, hy

189 Cellular membrane affinity chromatography is a technique that is based on

190 Cell separation based on microfluidic affinity chromatography is a widely used methodology in

191 Lectin affinity chromatography (LAC) has evolved as a powerful

192 In affinity chromatography, lengsin also bound the equivale

193 ibody and protein A with in-line multilectin affinity chromatography (M-LAC) which consists of three

194 rboxyl terminus of AbgB and subsequent metal affinity chromatography (MAC).

195 ules, which can be further purified by metal affinity chromatography (MAC).

196 DNA binding assays, liquid and affinity chromatography, MALDI-MS analysis, and de novo

197 ure in each candidate small molecule enables affinity chromatography-mass spectrometry, which produce

198 protein conjugates formed were identified by affinity chromatography-mass spectrometry.

199 IBA) column as a viable small-molecule-based affinity chromatography method for antibody purification

200 Here we describe a novel affinity chromatography method that utilizes the NBS as

201 A new affinity chromatography method was developed by modifyin

202 tion with titanium dioxide-based metal oxide affinity chromatography (MOAC).

203 Using FcRn affinity chromatography, molecular dynamics simulation,

204 , enzymatic assays, fluorescence microscopy, affinity chromatography, MS, and FRET analyses, we ident

205 Ni affinity chromatography of a mixture of E. coli (His)(6)

206 odextrin (beta-CD) is extremely effective in affinity chromatography of prenylated proteins.

207 All steps including immobilized metal ion affinity chromatography of protein on cobalt-loaded bead

208 Arginine-glycine-aspartic acid affinity chromatography of proteins from the ungerminate

209 By IL-34 affinity chromatography of solubilized mouse brain membr

210 ase-specific COMP fragments were isolated by affinity chromatography of synovial fluids from patients

211 Con A affinity chromatography of the Df extract revealed that

212 Here we used affinity chromatography of tumor cell extracts to identi

213 ctin was purified from the coelomic fluid by affinity chromatography on a GlcNAc-derivatized column a

214 ilized broad-spectrum kinase inhibitors, (3) affinity chromatography on immobilized (R)-roscovitine a

215 Using ammonium sulphate precipitation and affinity chromatography on pepstatin-A agarose bed the a

216 IgG was isolated from serum/plasma by affinity chromatography on Protein G.

217 ted proteins in the male genital tract using affinity chromatography on PSG2, an anti-sulfotyrosine m

218 lin from serum, involving PEG precipitation, affinity chromatography on Sepharose derivatized with ac

219 Purification of tumor homogenates by affinity chromatography on these peptides and subsequent

220 n this paper, an open-tubular capillary cell affinity chromatography (OT-CAC) method to enrich and se

221 tion system with open-tubular capillary cell affinity chromatography (OT-CAC), although any separatio

222 Compared to immobilized metal-ion affinity chromatography, PRISM provided approximately 10

223 ahistidines are very common tags used in the affinity chromatography purification of recombinant prot

224 ruct was further pursued for optimisation of affinity chromatography purification.

225 dem Mass Spectrometry (LC-MS/MS) analysis of affinity chromatography purified antigens resulted in id

226 f heterologously expressed and metal-chelate-affinity chromatography-purified ZmEXPB6 on growth-reduc

227 industrial scale, replacing fouled Protein A affinity chromatography resin accounts for a large propo

228 r device requires only microliter volumes of affinity chromatography resin-a condition that maximizes

229 B was coated onto an IMAC (immobilized metal affinity chromatography) resin and successfully bound Co

230 is by similarity search, protein arrays, and affinity chromatography revealed four native ligand-rece

231 itochondrial membrane of T. brucei by tandem affinity chromatography revealed that TbTim17 associates

232 dem affinity purification vector followed by affinity chromatography, SDS-PAGE, and identification of

233 can be improved by application of a combined affinity chromatography shotgun immunoproteomic approach

234 CaM-affinity chromatography showed that CaM binding to KCa3.

235 Affinity chromatography showed that the CendR peptides b

236 Affinity chromatography showed the binding of the TonB C

237 tial elution protocol from immobilized metal affinity chromatography (SIMAC) employing gallium-based

238 Overexpressed SHI was purified by a single affinity chromatography step using the Strep-tag II, and

239 eptide affinity just as antibodies, using an affinity chromatography strategy.

240 Optical methods include fluorescent frontal affinity chromatography, surface plasmon resonance metho

241 otein in mycobacterial species, and by using affinity chromatography techniques, we identify specific

242 urified from seabuckthorn seeds using chitin-affinity chromatography that showed antifreeze property

243 Using affinity chromatography, the authors identified Munc18-1

244 enrichment by Ub-affinity and nickel chelate-affinity chromatography, the ubiquitylated proteins were

245 Using RNA affinity chromatography, three members of the heterogene

246 an alpha(2)beta(2)gamma(2) hexamer by nickel affinity chromatography through a "His(6)" affinity tag

247 capillary isoelectric focusing and boronate affinity chromatography to assess the formation and diss

248 m BsAbs that were purified through protein A affinity chromatography to demonstrate industrial applic

249 phosphopeptide enrichment materials based on affinity chromatography to either immobilized metals (IM

250 etry-based workflow that incorporates lectin affinity chromatography to enrich for proteins that carr

251 on of titanium dioxide and immobilized metal affinity chromatography to enrich phosphopeptides, we ex

252 ded protein was then re-isolated by means of affinity chromatography to identify and quantify the cap

253 We subsequently used RNA affinity chromatography to identify Mbnl1 as a splicing

254 In this study, we used RNA affinity chromatography to identify trans factors that b

255 We have used affinity chromatography to isolate these antibodies and

256 Here, we used gelsolin affinity chromatography to purify actin from fission yea

257 ecombinant hSMVT in Pichia pastoris and used affinity chromatography to purify the detergent-solubili

258 geneous RNCs are isolated by combining metal affinity chromatography (to isolate ribosome-bound speci

259 Moreover, the chip could function as "affinity chromatography" to identify bioactive phytochem

260 peptide, was purified to near homogeneity by affinity chromatography under native conditions.

261 in was purified from the bacterial lysate by affinity chromatography using a biotinylated promoter re

262 ly, the hydrazide product can be enriched by affinity chromatography using aldehyde resins, thus dras

263 ding to the SOSIP.664 design and purified by affinity chromatography using broadly neutralizing antib

264 b) was separated and quantitated by boronate affinity chromatography using optimized shielding reagen

265 Affinity chromatography using patient-derived mAbs ident

266 phosphoethanolamine and could be purified by affinity chromatography using phosphoethanolamine-conjug

267 Metal affinity chromatography using polyhistidine tags is a st

268 Through affinity chromatography using primary neutrophil lysate,

269 change chromatographies and then purified by affinity chromatography using Sepharose-immobilized kiwi

270 To identify binding factors, we conducted affinity chromatography using the A-box element and foun

271 targets in an unbiased fashion, we performed affinity chromatography, using tandem liquid chromatogra

272 ile Cic was not identified through our A-box affinity chromatography, utilization of the same site, t

273 Affinity chromatography utilizing histidine tagged sV23

274 Weak affinity chromatography (WAC) was recently introduced as

275 Here, RNA affinity chromatography was used to isolate cellular pro

276 Additionally, tandem nickel-cobalt affinity chromatography was used to prepare highly purif

277 tal proteomics approach involving dye-ligand affinity chromatography, we confirm NTP binding to 47 di

278 Using HeLa cell extracts and DNA affinity chromatography, we demonstrate that RPA is pref

279 By using affinity chromatography, we find that Arl8-GTP binds to

280 Using affinity chromatography, we identified alpha- and beta-n

281 Using T(1)AM-affinity chromatography, we isolated this protein, and s

282 Using surface plasmon resonance and affinity chromatography, we show here that the second lu

283 Here, using a live cell assay and affinity chromatography, we show that huntingtin has a k

284 expression in yeast and purification by CaM-affinity chromatography, we show that, unlike other ACAs

285 IPR proteins isolated by I-peptide affinity chromatography were identified by proteomics as

286 glycated materials, separated using boronate affinity chromatography, were fully characterized using

287 The columns were characterized using frontal affinity chromatography with [(3)H]-epibatidine as the m

288 step method to purify native-like trimers by affinity chromatography with a trimer-specific bNAb, PGT

289 xpressed in Escherichia coli and purified by affinity chromatography with a yield of 100 mg/liter.

290 either fluorescence spectrometry or boronate affinity chromatography with detection by specific fluor

291 c acid (NMDA), was established using frontal affinity chromatography with flunitrazepam and diazepam

292 tone biotinylation, and enrichment by avidin affinity chromatography with mass spectrometry.

293 Here we used affinity chromatography with non-catalytic Coa and vWbp

294 in more weakly than wild-type as observed by affinity chromatography with scaffolding protein-agarose

295 Using RNA affinity chromatography with the 3'-untranslated region

296 that interact with this domain, we performed affinity chromatography with the recombinant intracellul

297 l antibodies by combining the selectivity of affinity chromatography with the simplicity of salt-indu

298 n of partially assembled F(O)F1 complexes by affinity chromatography with the use of mutants synthesi

299 action of CaBP4 and Unc119 was studied using affinity chromatography, yeast two-hybrid system, coimmu

300 the precursor protein, followed by negative affinity chromatography, yielded a purified peptide.