ライフサイエンスコーパス: affinity column

1 peptides and used it to create a methylysine-affinity column.

2 esulting protein was purified using a chitin affinity column.

3 and subsequently purified by a His antibody affinity column.

4 PfTopoII was purified using a DNA affinity column.

5 and purified to near homogeneity on a metal affinity column.

6 folded prior to or after binding to a Ni-NTA affinity column.

7 detergent-stable complex on a CD47 antibody affinity column.

8 kel-nitrilotriacetic acid resin and collagen affinity column.

9 identified as an RNA specie eluted from the affinity column.

10 rified through a pterin-conjugated Sepharose affinity column.

11 trices, including a pterin-6-carboxylic acid affinity column.

12 -NG, neither ox- nor PO(4)-NG bound to a CaM-affinity column.

13 ion of active recombinant enzyme on a nickel-affinity column.

14 tibody, are stacked to create a customizable affinity column.

15 scherichia coli and purified using an Ni(2+) affinity column.

16 cluding a proliferating cell nuclear antigen affinity column.

17 sion as His-tagged ZntR and purified by Ni2+-affinity column.

18 , and separation on a concanavalin A-agarose affinity column.

19 actor), from bovine brain by using a Src SH3 affinity column.

20 with immobilized alphavbeta3 receptor or the affinity column.

21 ody affinity column and an immobilized metal affinity column.

22 ingle chromatographic step over an anti-FLAG affinity column.

23 bin receptors were isolated using an inhibin affinity column.

24 of coat protein, and bound to a coat protein affinity column.

25 e-column chromatographies, including a G-DNA affinity column.

26 could specifically bind to a betaARK-coupled affinity column.

27 charides were chromatographed on a laminin-1 affinity column.

28 ed from COS cell membranes using an antibody affinity column.

29 ity-tagged intein remains immobilized on the affinity column.

30 g chromatography through an E2F-specific DNA affinity column.

31 n antibodies were purified using a recoverin-affinity column.

32 tly, PR were chromatographed on a mastoparan affinity column.

33 55-kDa protein on a vitellogenin mRNA 3'-UTR affinity column.

34 size, was obtained after binding to a GlcNAc affinity column.

35 adsorbed to and eluted from a GPIIIa-(49-66) affinity column.

36 CAT-sensitive binding of the ANT to a PheArs affinity column.

37 and by a wheat germ agglutinin (WGA) lectin affinity column.

38 agment was isolated and purified with an LDL affinity column.

39 on the pore wall facilitated its use in a CT affinity column.

40 r did it bind to an N-acetyl-D-galactosamine affinity column.

41 ds tightly to a carbonic anhydrase-Sepharose affinity column.

42 glycopeptides do not bind to a DGL-Sepharose affinity column.

43 unlike the reduced form, did not bind to CaM-affinity column.

44 bind to and elute from a DM-GRASP-Sepharose affinity column.

45 enriched in the high-salt eluates from a D' affinity column.

46 oblotting and through enrichment on a nickel affinity column.

47 were expressed in E.coli and purified by an affinity column.

48 for the I(2) subtype, 2-BFI, to generate an affinity column.

49 , RBC AChE solution was loaded on a Hupresin affinity column.

50 isolated, solubilized, and bound to rBPI(21) affinity column.

51 confirmed by using a scrambled DNA sequence affinity column.

52 DMP1-PG with a monoclonal anti-DMP1 antibody affinity column.

53 e yield using either a PGT145 or a 2G12 bNAb affinity column.

54 obratoxin column followed by a lentil lectin affinity column.

55 t cation-dependent manner to a type 1 repeat affinity column.

56 tibodies on protein A and beta-galactosidase affinity columns.

57 ng specific peptide elution from 14-3-3 zeta affinity columns.

58 nzae strains and used to prepare solid-phase affinity columns.

59 ype III but not type I collagen-Sepharose 2B affinity columns.

60 on of crude heparin retained on antithrombin affinity columns.

61 were specifically eluted from CS- conjugated affinity columns.

62 tted into an array of separable, multiplexed affinity columns.

63 was further purified on anti-apoE and apoA-I affinity columns.

64 y to bind pentamannosyl phosphate-containing affinity columns.

65 g activity from wild-type but not mutant RNA affinity columns.

66 ion of PP1-binding proteins on PP1-Sepharose affinity columns.

67 sily detectable binding to L- and P-selectin affinity columns.

68 ctionated into two pools based on binding to affinity columns.

69 y purified by acid/salt elution from antigen affinity columns.

70 Only 0.05-0.9% of IVGGs bound firmly to Id affinity columns.

71 ng SLE anti-DNA combining sites bound to Id+ affinity columns.

72 ther protein of the doublet bound to control affinity columns.

73 -A and anti-La/SS-B were isolated on antigen-affinity columns.

74 phatase holoenzyme (MBP) were compared using affinity columns.

75 could be eluted from monomeric IgG or HLA A2 affinity columns.

76 were applied to either anti-HA or anti-c-Myc affinity columns.

77 -FX cores on both G-75 gel filtration and Ni affinity columns.

78 sed in Escherichia coli and purified on Flag-affinity columns.

79 ated sCD44 was tested in cell culture and HA affinity columns.

80 ssing cells were fractionated using a CI-MPR affinity column, 35-45% of the total LIF molecules were

81 to a biotinylated hyaluronan binding protein affinity column, also showed monocyte chemotactic activi

82 from E. chaffeensis lysate using penicillin affinity column and a complementation assay confirmed cy

83 roceeding with an ACAT-1 monoclonal antibody affinity column and an immobilized metal affinity column

84 esultant nanoMIP beacons were eluted off the affinity column and characterised using transmission ele

85 he labeled receptor was purified on a nickel affinity column and cleaved with factor Xa protease at a

86 Affinity column and colocalization experiments showed th

87 teins, we used a LANA binding sequence 1 DNA affinity column and determined the identities of a numbe

88 copurifies with CIRL on an alpha-latrotoxin affinity column and forms stable complexes with this rec

89 rA and CcdB; this complex can be detected by affinity column and gel-shift analysis, and has a proteo

90 The isozyme bound to a dimethenamid-affinity column and had a subunit molecular mass of 26 k

91 tely 42 kDa was specifically adsorbed by the affinity column and identified as ERK1 and ERK2.

92 In this study, p42 was purified using an RNA affinity column and identified as TIAR by peptide sequen

93 tic peptides were purified on a streptavidin affinity column and identified by mass spectrometry.

94 the RecA intein was purified on a metal ion affinity column and renatured in the presence of 2 mM Zn

95 bsequently purified through a 6xHis-Ni2+-NTA affinity column and SP Sepharose/fast protein liquid chr

96 t enzymes were purified to homogeneity on an affinity column and their biochemical characteristics we

97 es were purified on a folate-binding protein affinity column and then applied to a Sephadex G-10 colu

98 mutants bound less strongly to a tropomyosin affinity column and were less able to stabilize the TM o

99 vidin and other biotin-binding protein-based affinity columns and are recognized by anti-biotin antib

100 romatography of parasite extracts on mannose affinity columns and by immunohistochemical and Western

101 ted by the binding of Nox5 to phosphoprotein-affinity columns and via MS/MS analysis.

102 separation of the neuramindase with a lectin affinity column, and addition of synthesized 13C-CMP-Neu

103 igand complex was purified using a metal ion affinity column, and after cyanogen bromide treatment, a

104 , the 33-kDa product bound to a NADP-agarose affinity column, and could be eluted with a buffer conta

105 g His-tagged H5, chromatographed on a nickel affinity column, and eluted using an imidazole gradient.

106 unoreactivity to calreticulin, binds a hep I affinity column, and neutralizes thrombospondin/hep I-me

107 mbinant proteins were purified through a His affinity column, and their biological properties were an

108 ivity from rabbit brains using a GTP-agarose affinity column, and this protein stimulates PLD activit

109 obilized via a capture oligonucleotide on an affinity column, and those variants that could be releas

110 inactive, were unable to bind to a substrate affinity column, and were not secreted from Sf9 cells.

111 canavalin A and wheat germ agglutinin lectin affinity columns, and PNGase-F digestion released most o

112 iment demonstrates that such chemical methyl-affinity columns are capable of enriching and improving

113 second activity is achieved by use of an RNA-affinity column based on the HRV 5' UTR.

114 SBEIIa and SBEIIb also were retained on an affinity column bearing a specific conserved fragment of

115 An oligonucleotide affinity column bearing the P7I target site purifies a 2

116 This allows for a facile regeneration of the affinity column because the phenothiazine-silica support

117 Yeast two-hybrid studies and affinity column binding assay show that the isolated AT(

118 molecular mass but not the step required for affinity column binding, suggesting that enzyme activati

119 CaM in blot overlay assays and binds to CaM-affinity columns, both only in the presence of 10 microM

120 2gamma2HF dimers immobilized on an anti-FLAG affinity column bound all five alpha subunits tested, wh

121 A Tat-affinity column bound one transcription factor, Tat-SF1,

122 The second remained bound to the affinity column but could be eluted as a mixture of Vh,

123 emoglobin complexes did not bind to boronate affinity columns but instead eluted intact in A1c1 and A

124 g chimera, AAV2G9, continues to bind heparin affinity columns but interchangeably exploits Gal and he

125 Wild type ALAS2 bound strongly to a SUCLA2 affinity column, but the adjacent XLSA mutant enzymes an

126 ants are unfolded upon eluting from the (2)D affinity column by postcolumn addition of a denaturing s

127 characterizing their enrichment on taipoxin affinity columns; by expressing NP1, NP2, and NPR singly

128 CheB bound specifically to an affinity column carrying the isolated pentapeptide, impl

129 ns contaminating proteins eluted from Ni(2+) affinity columns cause rapid oxidation of DTT without af

130 rix p13-agarose, and Cdc28 is retained on an affinity column charged with bacterially produced Cdc6.

131 Affinity columns charged with recombinant hnRNP K specif

132 e activity and sequential ATP and calmodulin affinity column chromatography analyses reveal that skel

133 bodies and immune complexes were purified by affinity column chromatography and analyzed by 2-D gel e

134 cted polypeptide was purified by glutathione affinity column chromatography and characterized.

135 H-B, were purified to homogeneity by His-tag affinity column chromatography and used to immunize rabb

136 By using DNA affinity column chromatography containing the cis-acting

137 We identified alphaCP3 using affinity column chromatography containing the double-str

138 Immunoprecipitation and affinity column chromatography demonstrated direct bindi

139 Co-immunoprecipitation and affinity column chromatography experiments demonstrate t

140 pxDeltaC and SpxDeltaCHA followed by anti-HA affinity column chromatography of a cleared lysate resul

141 Ligand-affinity column chromatography of detergent-soluble memb

142 PEDF affinity column chromatography of membrane proteins from

143 The expressed proteins were purified by Ni-affinity column chromatography to yield active GTR.

144 purified from kidney homogenates by heparin affinity column chromatography using elution with a 0.2-

145 Vnd/NK-2 homeodomain affinity column chromatography was used to purify Drosop

146 ar homogeneity using Fn-coupled Sepharose 4B-affinity column chromatography, and amino acid sequence

147 glutathione S-transferase co-precipitation, affinity column chromatography, and immunoprecipitations

148 p random sequences were purified by vnd/NK-2 affinity column chromatography, cloned, and sequenced.

149 entional purification techniques, especially affinity column chromatography, have been instrumental i

150 n of the Abs from IGIV via fibril-conjugated affinity column chromatography, the EC50-binding value f

151 Using Tax affinity column chromatography, we demonstrate that Tax

152 By establishing an affinity column chromatography-based assay using the mon

153 en LNCaP cell extracts were subjected to CaM-affinity column chromatography.

154 s to type I, but not to type III collagen by affinity column chromatography.

155 e fusions purified using glutathione-agarose affinity column chromatography.

156 ter-based pET30 expression vector and nickel affinity column chromatography.

157 His-tagged GTR protein was purified using Ni affinity column chromatography.

158 combinant EWS-FLI1 in Escherichia coli using affinity column chromatography.

159 DEK autoantibodies and ICs were purified by affinity-column chromatography and analyzed by 2-dimensi

160 Sera were applied to affinity columns coated with GAD65-specific mAbs to abso

161 HeLa whole cell extracts by binding to a RNA affinity column comprised of vimentin's 3'UTR.

162 the binding of purified p53 to the AAV Rep78 affinity column confirms their interaction.

163 s an internal standard and with the use of 2 affinity columns connected in parallel to the analytic c

164 hondroitin sulfate, bind to type IV collagen affinity columns, consistent with a role for CD44/CSPG-t

165 ation of mitochondrial extracts on MBP-RBP16 affinity columns consistently isolated proteins of 12, 1

166 ly to phosphorylated beta(3), we utilized an affinity column consisting of a peptide modeled on the t

167 hemagglutinin antigen epitope binds to a DNA affinity column containing covalently linked tandem repe

168 Recently, an affinity column containing the broadly neutralizing anti

169 y, depletion of a nuclear extract with a DNA affinity column containing the U6 PSE sequence reduces e

170 Furthermore, GLUT4 bound to a peptide affinity column containing the zHFF sequence and was elu

171 define further this effect on GroEL binding, affinity columns containing a variety of denatured prote

172 ntify cellular targets of Rdx proteins using affinity columns containing mutant versions of these pro

173 targets of TRs in mammalian cells utilizing affinity columns containing recombinant TR3 forms differ

174 w a strong retention on a synthetic boronate affinity column, containing sulfonamide-phenylboronic ac

175 sted of three size exclusion columns and one affinity column coupled to inductively coupled plasma ma

176 A phospho-carboxyl-terminal domain (CTD) affinity column created with yeast CTD kinase I and the

177 Immunoprecipitation of the affinity column eluate with a Galpha(13) antibody demons

178 Among five major polypeptides in the affinity column eluate, the activity of interest was ass

179 he excess high-affinity binding sites on the affinity columns enable quantitative extraction of 5 MT

180 1-11 cells, solubilized, and bound to nickel affinity columns, establishing their physical associatio

181 lyzed LNCaP cell extracts that bind to a CaM affinity column for the presence of low molecular weight

182 eceptor-bearing microspheres, sequestered in affinity column format inside a microfluidic channel.

183 Rs were purified on an alphaBgTx-derivatized affinity column from detergent extracts in milligram qua

184 nocytic cells were chromatographed on a CRID affinity column, GST Omega 1-1 bound selectively to the

185 ht complex with actin, and deoxyribonuclease affinity columns have been utilized to identify a variet

186 s was observed with eluates from anti-DNA Id affinity columns; however, no correlation between IVGG a

187 folium L. by consecutive passage through two affinity columns, i.e. asialofetuin-Sepharose and invert

188 KSHV TR DNA and the LANA binding site as the affinity column identified topoisomerase IIbeta (TopoIIb

189 ung tissue [with a human serum albumin (HSA)-affinity column] identified these albumin-binding protei

190 recombinant kinesin-C binds to a calmodulin-affinity column in a Ca(2+)-dependent fashion.

191 This platform includes a boronate affinity column in the first dimension for enrichment, r

192 l lysates were loaded onto the phenothiazine affinity column in the presence of a Ca(2+)-containing b

193 ized HUVEC membranes were subjected to an HK-affinity column in the presence or absence of 50 microM

194 lls and that both mucins bound to galectin-3 affinity columns in a galactose-dependent manner.

195 etained on recombinant GST-tagged Myo5a tail affinity columns in a GTP-dependent manner.

196 of the mitochondrial extracts to mutant Trx affinity columns in conjunction with proteomics led to t

197 a crude bovine brain extract to bind to GRK affinity columns in the absence or presence of AlF(4)(-)

198 d malignant tumors using antibody- or lectin-affinity columns in the presence of 5 mmol/L EDTA were a

199 Also, HLA-B2705 purified using the mAb ME1 affinity column includes this unique mAb MARB4-reactive,

200 ysis following EDTA elution from a D-Hep-III affinity column indicated that D-Hep-III binds to the al

201 cisplatin intrastrand and ICL containing DNA affinity columns is comparable.

202 P fusion proteins from cell extracts, and as affinity column ligands for inexpensive large-scale prot

203 IVGG was adsorbed to Sepharose 4B affinity columns linked to a panel of cationic human IgG

204 onally, the polymer bound specifically to an affinity column made with a type 3 polysaccharide-specif

205 lated from bovine tongue epithelium using an affinity column made with an antibody to the cornified e

206 ese findings indicate that the LZ-linked AGE affinity column may serve as an efficient method for the

207 yoPearl resin resulting in the NBS targeting affinity column (NBS(IBA)).

208 A nitrotyrosine affinity column (NTAC) was used to preferentially enrich

209 timulated platelets binds specifically to an affinity column of 4N1K peptide.

210 uencing of a protein obtained by benzamidine affinity column of Plasmodium gallinaceum ookinete axeni

211 diator can quantitatively bind to an E1A-CR3 affinity column, only on the order of 1% of cellular Med

212 moglobin from patient CSF with a haptoglobin-affinity column or its sequestration in a soluble hemogl

213 Using a dynein affinity column, p22, the smallest dynactin subunit, was

214 Here, we employ a LECT2-affinity column plus liquid chromatography coupled with

215 nti-F(ab')2 antibodies by adsorption with an affinity column prepared with normal IgG F(ab')2.

216 The protein was refolded on the metal-affinity column prior to elution.

217 nuclear extract was separated on the methyl-affinity column prior to standard proteomics analysis.

218 Affinity column purification supported the specificity o

219 ly from ligation reaction mixtures by Ni-NTA affinity column purification.

220 Here we show that affinity column-purified protein encoded by human ARD-1

221 Affinity column-purified UL84-FLAG fusion protein was us

222 from CHO cell conditioned media with a G-CSF affinity column, resting in a preparation fully competen

223 Using an affinity column retention assay, we showed that the puri

224 e purified from bacterial extracts on a YajL affinity column, separated by nonreducing-reducing SDS-P

225 Supporting this, a dermaseptin S3(1-16) affinity column specifically purified Stm1p, Mre11p and

226 ogical counterreceptor MUC16 from galectin-3 affinity columns, suggesting that association of transme

227 and 34/31-kDa polypeptides in eluates of CaM affinity columns, suggesting the presence of CaM-binding

228 PCBP2 by passage over a PV stem-loop IV RNA affinity column supported only low levels of HAV RNA tra

229 forms of pFNRII bound more strongly to a Fd affinity column than did the shorter forms, pFNRII(ISKK)

230 (iii) Ndk failed to bind to a Ugi-Sepharose affinity column that tightly bound E. coli uracil-DNA gl

231 a biotin-high molecular mass kininogen (HK) affinity column that, on aminoterminal sequencing of try

232 pecies of 55 kDa (synaptegrin-1) from GRGDSP-affinity columns that had been loaded with solubilized s

233 Using a dynein affinity column, the previously uncharacterized p62 subu

234 or diabetic lenses were bound to a boronate affinity column, the protein-containing gel was incubate

235 By exploiting receptor affinity columns, the LT-alpha3, LT-alpha2/beta1, and LT

236 To address these issues, we used a ClpS affinity column to isolate interacting proteins from E.

237 In this study, we used an affinity column to purify proteins that selectively bind

238 APS was purified on concanavalin A affinity columns to minimize the loss of the anchoring s

239 aced by serine or alanine, and bound them to affinity columns to trap target proteins of chloroplast

240 Cpefat activity does not bind to a substrate affinity column under conditions that bind CPE.

241 rified successfully via a positive-selection affinity column using the bNAb PGT145, which recognizes

242 cell proteins that were eluted from a 14-3-3 affinity column using the phosphopeptide ARAApSAPA.

243 Affinity columns using actin monomers (globular actin, G

244 the one-step fabrication and application of affinity columns using reversibly immobilized antibodies

245 omycetemcomitans serotype b O-polysaccharide affinity column was constructed and subsequently used to

246 An affinity column was constructed with the recombinant ext

247 ectivity of a commercially available heparin affinity column was investigated for chloroquine.

248 Alpha-gliadin affinity column was loaded with intestinal mucosal membr

249 The affinity column was prepared by coupling indole butyric

250 end covalently to oxirane acrylic beads, an affinity column was prepared for selective removal of RN

251 An affinity column was prepared using the recombinant fragm

252 Eluted protein from this antibody-affinity column was recognized by antibodies directed ag

253 An S. mutans affinity column was used to isolate active moieties from

254 A HIV-1 Nef affinity column was used to purify a 35-kDa Nef-interact

255 A BUR affinity column was used to purify a doublet of Mr 20,00

256 branes associated with wheat germ agglutinin-affinity columns, was [3H]galactose-labeled with UDP-[3H

257 f IEBP isolated from a 17beta-estradiol (E2) affinity column we cloned a full-length cDNA for IEBP fr

258 Using an acetylcholine-derivatized affinity column, we have purified human alpha4beta2 neur

259 Using an LPL RNA affinity column, we identified two of the RNA-binding pr

260 Using an NKX3.1 affinity column, we isolated topoisomerase I (Topo I) fr

261 A phage display screen using Plk1 C-terminal affinity columns, we identified NudC (nuclear distributi

262 Using four affinity columns, we isolated IgG anti-Ids to anti-P ant

263 s that co-purified with ABCA1 on an antibody affinity column were identified by liquid chromatography

264 Proteins eluted from the NF-kappaB affinity column were subjected to proteomic analysis and

265 Proteins that bound specifically to the affinity columns were co-eluted in a complex with beta-p

266 that specifically bound and eluted from the affinity columns were identified by microcapillary high

267 G-actin, F-actin and bovine serum albumin affinity columns were prepared and used to separate samp

268 ged carriers were in the flow-through of the affinity column, whereas all of the tagged carriers boun

269 ofilin kinase binds to a deoxyribonuclease I affinity column, whereas the nonspecific cofilin kinase

270 ditioned medium was depleted with an activin-affinity column which binds follistatin.

271 t describes the generation of an alphavbeta3 affinity column which was created to enable screening of

272 atured in 8 M urea, and again applied to the affinity column, which then hound Vh but not LcrH.

273 n chromatography, including two epsilonC-DNA affinity columns, which resulted in >1,000-fold purifica

274 ract from bovine testis was applied to these affinity columns, which were then extensively washed.

275 TGFL eluted from the GFL receptor/protein G affinity column with 0.5 M NaCl, pH 7.5, and potently pr

276 Second, following recovery from the affinity column with an acidic wash, mAb quality is asse

277 These proteins were purified by RNA affinity column with biotinylated negative-strand MHV le

278 An affinity column with covalently bound Glued protein reta

279 Treating the material bound to the affinity columns with heparitinase and chondroitinase en

280 Clarified serum is loaded directly onto the affinity column without prior adjustment and albumin and

281 also developed based on a copper immobilized affinity column without the addition of any affinity tag

282 taCHA, and RNAP, when applied to the anti-HA affinity column, yielded only inactive Spx(R60E)DeltaCHA