ライフサイエンスコーパス: aldolase

1 defect is rescued by expression of exogenous aldolase.

2 retains the known F-actin binding ability of aldolase.

3 ate dehydrogenase, malate dehydrogenase, and aldolase.

4 hen becomes a substrate of a Neu5Ac-specific aldolase.

5 substrate tolerance is unprecedented for an aldolase.

6 id changes from the wild-type E. coli KDPGal aldolase.

7 olase, and 2-keto-3-deoxy-6-phosphogluconate aldolase.

8 a representative member of this family, FBP aldolase.

9 rded as an organocatalytic mimic of fuculose aldolase.

10 y occurring enzyme fructose 1,6 bisphosphate aldolase.

11 5Ac (N-acetylneuraminic acid, D-sialic acid) aldolase.

12 cose is split into 3-carbon intermediates by aldolase.

13 hoglucose isomerase, phosphofructokinase and aldolase.

14 d 2-keto-3,6-dideoxy-6-sulfogluconate (KDSG) aldolase.

15 ases yet similar to metal-dependent class II aldolases.

16 Schiff base with the substrate, unlike most aldolases.

17 ual N-terminal extensions not found in other aldolases.

18 the generated series of the artificial retro-aldolases.

19 cement that rivals the efficiency of class I aldolases.

20 s involved being parvalbumins, enolases, and aldolases.

21 late intermediates (4-hydroxy-2-ketovalerate aldolase, 2-isopropylmalate synthase, and transcarboxyla

22 ved for carbonic anhydrase (29 kDa), 50% for aldolase (39 kDa), 46% for enolase (46 kDa), and 27% for

23 analysis we identified the glycolytic enzyme aldolase A (AldoA) as a binding partner of CAPN3.

24 ytic enzymes we evaluated by gene silencing, aldolase A (ALDOA) blockade produced the most robust dec

25 is study, we show that the glycolytic enzyme aldolase A (ALDOA) is a key enzyme involved in lung canc

26 io of kcat/Km for the two substrates between aldolase A and aldolase B.

27 yruvate kinase, isoforms of creatine kinase, aldolase A and an isoform of glyceraldehyde 3-phosphate

28 Mechanistically, Rac1 activates aldolase A and ERK signaling which up-regulates glycolys

29 This study demonstrates the role of aldolase A and its interaction with gamma-actin in the m

30 y, we show that two host glycolytic enzymes, aldolase A and pyruvate kinase, as well as lactate dehyd

31 o provide a preclinical rationale to develop aldolase A inhibitors as a generalized strategy to treat

32 est IgE reactivity to collagen, tropomyosin, aldolase A or beta-enolase but not parvalbumin.

33 Depletion of the host glycolytic enzyme aldolase A resulted in decreased inclusion size and infe

34 with a specific small-molecule inhibitor of aldolase A was sufficient to increase overall survival i

35 ned two additional potential food allergens 'aldolase A' and 'thioredoxin h'.

36 we identified glycolytic enzymes (GAPDH and aldolase A) as putative interacting proteins.

37 skeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity.

38 ur previously untargeted glycolytic enzymes, aldolase A, glyceraldehyde 3-phosphate dehydrogenase, tr

39 formational flexibility has been observed in aldolase A, its function in the catalytic reaction of al

40 Interactions of aldolases A and C in NF-L expression may be linked to re

41 Aldolases A and C, but not B, interact specifically with

42 Fructose-1,6-bisphosphate (FBP) aldolase, a glycolytic enzyme, catalyzes the reversible

43 Aldolase, a glycolytic enzyme, must distinguish between

44 )beta3 and alpha(v)beta5, and the monoclonal aldolase Ab 38C2.

45 Knockdown of aldolases activates AMPK even in cells with abundant glu

46 , suggesting that both the plasticity of the aldolase active-site region and the multimeric nature of

47 tryptophan led to enzymes with no detectable aldolase activity.

48 confirming that it has fructose bisphosphate aldolase activity.

49 teins exhibited significant dihydroneopterin aldolase activity.

50 range of protein folds, had detectable retro-aldolase activity.

51 s actin-bound aldolase A, and an increase in aldolase activity.

52 LsrF, despite strong structural homology to aldolases, acts as a thiolase, an activity previously un

53 sing SAD phasing and belongs to the class II aldolase/adducin superfamily.

54 is and comparison of the enzyme with related aldolases, ADH synthase is classified as a new member of

55 Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with dive

56 model posits that fructose-1,6-bisphosphate aldolase (ALD) provides a critical link between the cyto

57 on of PFK-1.1 condensates and recruitment of aldolase/ALDO-1.

58 pecificity among fructose-1,6-(bis)phosphate aldolase (aldolase) isozymes.

59 cleavage to pyruvate and L-lactaldehyde via aldolase and (iv) L-lactaldehyde conversion to L-lactate

60 or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (

61 ctural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse funct

62 Both aldolase and CAPN3 are present in the triad-enriched fra

63 4 fish allergens (parvalbumin, tropomyosin, aldolase and collagen).

64 Chicken parvalbumin and two new allergens, aldolase and enolase, were identified at 12, 40, and 50

65 ythritol as the sole C source should require aldolase and fructose-1,6-bisphosphatase to produce esse

66 nd aminoethanol using D-fructose-6-phosphate aldolase and L-rhamnulose-1-phosphate aldolase catalysts

67 cal interactions with the glycolytic enzymes Aldolase and Phosphoglycerate mutase.

68 biomarkers (myosin-9, fructose-bisphosphate aldolase and plectin).

69 The catalytic activities of aldolase and pyruvate kinase functionally modulate K(ATP

70 ing, respectively, fructose 1,6-bisphosphate aldolase and pyruvate kinase, under the same conditions.

71 Interaction between aldolase and SUR was confirmed using GST pulldown assays

72 nts shows that disruption of binding between aldolase and the B subunit of V-ATPase results in disass

73 ortant role for physical association between aldolase and V-ATPase in the regulation of the proton pu

74 te and dehydration of the Schiff base in FBP aldolase and, by analogy, the class I aldolase family.

75 ral feature of biological catalysts, such as aldolases and small molecule amine organocatalysts.

76 tine kinase, aspartate aminotransferase, and aldolase) and inversely correlated with the duration of

77 n, glyceraldehyde-3-phosphate dehydrogenase, aldolase, and aspartate aminotransferase and thus reacti

78 trations of histidine-rich protein 2 (HRP2), aldolase, and lactate dehydrogenase (LDH) antigens were

79 substrates of members of the PdxA, class II aldolase, and RuBisCO superfamilies are phosphorylated,

80 olase enzymes and recent studies on designed aldolase antibodies and organocatalysts, direct structur

81 intermediate in the crystal structure of an aldolase antibody 33F12 in complex with a 1,3-diketone d

82 Interactions of SNX9 with aldolase are far more extensive and differ from those of

83 Crystals of AP-aldolase are grown at two temperatures (4 degrees C and

84 Aldolases are C-C bond forming enzymes that have become

85 Intriguingly, both aldolases are competent to bind polymerized actin in vit

86 Thus, stereocomplementary class II pyruvate aldolases are now available to create chiral 4-hydroxy-2

87 mechanisms that direct the functions of the aldolase as a scaffold protein.

88 The identification of aldolases as methyl proteins in Arabidopsis and other sp

89 These results establish that aldolase, as well as being a glycolytic enzyme, is a sen

90 gesting that PABP shields the NF-L mRNA from aldolase attack.

91 Loss of hepatic fructose-1, 6-bisphosphate aldolase B (Aldob) leads to a paradoxical up-regulation

92 ror in metabolism caused by mutations in the aldolase B gene, which is critical for gluconeogenesis a

93 or the two substrates between aldolase A and aldolase B.

94 glycolytic enzyme fructose-1,6-bisphosphate aldolase (BB0445), the Borrelia oxidative stress regulat

95 upstream acidic region is not necessary for aldolase binding but is nonetheless essential to parasit

96 y using alanine point mutants to investigate aldolase binding in vitro and to test functionality in t

97 nultimate tryptophan, which is essential for aldolase binding, and clustered acidic residues.

98 ibits E. coli class II fructose bisphosphate aldolase, but not RNA polymerase.

99 to improve a previously optimized artificial aldolase by an additional factor of 30 to give a >10(9)

100 els of mRNAs encoding the glycolytic enzymes aldolase C (AldoC, also known as zebrin II) and phosphof

101 Moreover, the isoenzyme aldolase C [also known as zebrin II (ZII)] is heterogene

102 A specific in vivo interaction between aldolase C and NF-L mRNA had been localized to a 68 nt s

103 mpetitive interactions in cells coexpressing aldolase C and NF-L.

104 f mRNA decay has assessed mechanisms whereby aldolase C and PABP control NF-L expression.

105 The multifunctional proteins aldolase C and poly (A)-binding protein (PABP) undergo c

106 This model shows that aldolase C is a zinc-activated ribonuclease that cleaves

107 th their expression of the glycolytic enzyme aldolase C or zebrin.

108 n spinal cord pulls down the dimeric form of aldolase C suggesting that their co-regulation of NF-L e

109 cells that express high levels of zebrin II (aldolase C) and the glutamate transporter EAAT4 cluster

110 Zebrin II (ZII; a.k.a. aldolase C) is expressed heterogeneously in Purkinje cel

111 Zebrin II (aldolase C) is expressed in a subset of Purkinje cells i

112 ck protein 10 (Hsp10), fructose bisphosphate aldolase C, and NADH-ubiquinone oxidoreductase as protei

113 ase-related protein 2, fructose-bisphosphate aldolase C, chaperonin-containing T-complex polypeptide

114 in, complement C9, gelsolin, testican-2, and aldolase C, performed well in a training set (area under

115 with unrelated sequence, is not degraded by aldolase C.

116 d be linked to the oligomerization status of aldolase C.

117 hoglycerate kinase 1 (PGK 1), alpha enolase, aldolase C/Zebrin II) were included among the axonally s

118 The trans-o-hydroxybenzylidene pyruvate aldolase-catalysed reactions between fluoropyruvate and

119 Finally, in the third case, aldolase catalysis was employed for synthesis of the cor

120 sphate aldolase and L-rhamnulose-1-phosphate aldolase catalysts, respectively.

121 cement of either of these residues decreased aldolase catalytic activity at least 400-fold.

122 d on using the chemistry of the well studied aldolase catalytic antibodies of which mAb 38C2 is a mem

123 ymatically modified derivatives, sialic acid aldolase-catalyzed condensation reaction leads to the fo

124 In addition, a cascade of three aldolase-catalyzed reactions enables one-pot assembly of

125 BphI, a pyruvate-specific class II aldolase, catalyzes the reversible carbon-carbon bond fo

126 Of these enzymes, knockdown of aldolase causes the greatest effect, inhibiting cell pro

127 P) to SBP, after which fructose-bisphosphate aldolase cleaves SBP into dihydroxyacetone phosphate and

128 Our aldolase collection is then employed in the chemoenzymat

129 ata from top-down MS of native and denatured aldolase complexes, a total of 56% of the total backbone

130 Aldolase connects the motor actin filaments to transmemb

131 of glFBPA, whereas tagatose-1,6-bisphosphate aldolase contains an alanine in this position.

132 the homologous 2-dehydro-3-deoxygalactarate aldolase, coupled with site-directed mutagenesis data, i

133 The growth of T. gondii aldolase crystals in acidic conditions enabled trapping

134 pped in the active site of Toxoplasma gondii aldolase crystals to high resolution.

135 the homologous 2-dehydro-3-deoxygalactarate aldolase (DDGA).

136 d acceleration, with deoxyribose-5-phosphate aldolase (DERA) achieving an average 15-fold enhancement

137 ntermediate is produced using a deoxy ribose aldolase (DERA) enzyme in which two carbon-carbon bonds

138 ldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA).

139 (KDGP), which is subsequently cleaved by the aldolase DgaF to form glyceraldehyde-3-phosphate and pyr

140 The enzymes dihydroneopterin aldolase (DHNA) and 6-hydroxymethyl-7,8-dihydropterin py

141 Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydron

142 Dihydroneopterin aldolase (DHNA) catalyzes the conversion of 7,8-dihydron

143 e, as no candidate gene for dihydroneopterin aldolase (DHNA) could be identified.

144 The gene encoding 7,8-dihydroneopterin aldolase (DHNA) was recently identified in archaea throu

145 es of progressively larger substrates to the aldolase, DmpG, using molecular dynamics.

146 Large proteins (single aldolase domain and albumin) now follow, with no broad b

147 ins, a flavin reductase-like protein, and an aldolase, each located in thylakoid-associated plastoglo

148 ersion of Thr to Gly and acetaldehyde by Thr aldolase (EC 4.1.2.5) was only recently shown to play a

149 Here, we report that the type II HpcH aldolases efficiently catalyze fluoropyruvate addition t

150 In contrast, aldolase enzymatic activity is not required for V-ATPase

151 We have used the aldolase enzyme as a model protein to conduct our studie

152 ades of investigation of naturally occurring aldolase enzymes and recent studies on designed aldolase

153 talytic antibody than speculated for natural aldolase enzymes and should serve to guide future studie

154 of the divalent metal ion dependent class II aldolase enzymes that have great biosynthetic potential.

155 6) created a series of five artificial retro-aldolase enzymes via directed evolution, with the final

156 In a fashion analogous to aldolase enzymes, the de novo preparation of L-ribulose,

157 Mimicking the actions of aldolase enzymes, the synthesis of selected carbohydrate

158 Aldolase exists as three isozymes, A, B, and C, distingu

159 the LC4 motif of human SNX9 in complex with aldolase explains the biochemistry and biology of this i

160 strategy consists of L-fuculose-1-phosphate aldolase F131A-variant-catalyzed aldol addition of dihyd

161 yase family has been included in the class I aldolase family on the basis of similar Schiff-base chem

162 ) is a member of the class II zinc-dependent aldolase family that catalyzes the cleavage of d-fructos

163 defined in the SCOPS database as the class I aldolase family.

164 in FBP aldolase and, by analogy, the class I aldolase family.

165 The class IIa fructose 1,6-bisphosphate aldolase (FBA) enzyme from M. tuberculosis (MtFBA) has b

166 ociation and included: fructose-bisphosphate aldolase (Fba); methyltetrahydropteroyltriglutamate (Met

167 dependent class II fructose-1,6-bisphosphate aldolase (FBA-tb), a key enzyme of gluconeogenesis absen

168 Fructose-1, 6-bisphosphate aldolases (FBA) are cytoplasmic glycolytic enzymes, whic

169 f nickel toxicity in E. coli as the class II aldolase FbaA through binding to the non-catalytic zinc

170 sults suggest that fructose-1,6-bisphosphate aldolase (FbaA) is a target of nickel toxicity.

171 the glycolytic enzymes fructose bisphosphate aldolase (FBPA) and glyceraldehyde-3-phosphate dehydroge

172 Giardia lamblia fructose-1,6-bisphosphate aldolase (FBPA) is a member of the class II zinc-depende

173 ass I and class II fructose-1,6-bisphosphate aldolases (FBPA), glycolytic pathway enzymes, exhibit no

174 Dihydroneopterin aldolase (FolB) catalyzes conversion of dihydroneopterin

175 functionalization of the classic TIM barrel aldolase fold.

176 2-keto-3-deoxy-6-phosphogalactonate (KDPGal) aldolase for microbial synthesis of shikimate pathway pr

177 ribed here uses recombinant His6-tagged KDPG aldolase for the synthesis of (S)-4-hydroxy-2-keto-4-(2'

178 light the chemical versatility of artificial aldolases for the practical synthesis of important chira

179 Previous studies have shown that aldolase forms a critical bridge between actin filaments

180 An aldolase from the bile acid-degrading actinobacterium Th

181 that photosynthetic eukaryotes acquired KDPG aldolase from the cyanobacterial ancestors of plastids v

182 Active KDPG aldolases from the cyanobacterium Synechocystis and the

183 pecific aldol addition catalyzed by pyruvate aldolases from the Entner-Doudoroff and the DeLey-Doudor

184 The family includes the "classical" aldolases fructose-1,6-(bis)phosphate (FBP) aldolase, tr

185 the recently discovered fructose-6-phosphate aldolase (FSA), which is functionally distinct from know

186 These WHO elements home into the aldolase gene FBA1, replacing its 3' end each time they

187 protein (IbpA) and a putative allo-threonine aldolase (GlyI).

188 This class II aldolase has an active site zinc and a non-catalytic zin

189 A, its function in the catalytic reaction of aldolase has not been demonstrated.

190 Aldolases have emerged as key enzymes involved in these

191 ved from a class I tagatose-1,6-bisphosphate aldolase homologous to those involved in lactose and gal

192 uctures of divalent metal-dependent pyruvate aldolase, HpaI, in complex with substrate and cleavage p

193 putative 4-hydroxy-2-ketoheptane-1,7-dioate aldolase (HpcH) in the sequence databases.

194 alyzed by 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (HpcH), a member of the divalent metal ion depe

195 relative to that of wild-type E. coli KDPGal aldolase in catalyzing the addition of pyruvate to d-ery

196 me reaction as that catalyzed by Eda, a KDGP aldolase in the Entner-Doudoroff pathway, and the two en

197 membrane protein 1 (TgAMA1), which binds to aldolase in vitro.

198 ), which is functionally distinct from known aldolases in its tolerance of different donor substrates

199 ic properties and tetrameric organization of aldolases in vitro.

200 nt absence of a key enzyme, dihydroneopterin aldolase, in the classical folate biosynthetic pathway o

201 Second, biochemical studies showed that aldolase indeed catalyzed these reactions.

202 ose of the actin-nucleating factor WASP with aldolase, indicating considerable plasticity in mechanis

203 The TRAP-aldolase interaction is a distinctive and critical trait

204 n mechanism associated with metallodependent aldolases involving recruitment of the catalytic zinc io

205 m Mycobacterium tuberculosis, the T. curvata aldolase is a protein complex of Ltp2 with a DUF35 domai

206 The aldehyde intermediate produced by the aldolase is channeled directly through a buried molecula

207 Accordingly, SNX9 binding to aldolase is structurally precluded by the binding of sub

208 , 2-keto-3-deoxygluconate-6-phosphate (KDPG) aldolase, is widespread in cyanobacteria, moss, fern, al

209 ciated with the variable activities of human aldolase isoenzymes modulated LacD.1's affinity for subs

210 are chloroplastic fructose 1,6-bisphosphate aldolase isoforms.

211 provides the first evidence for three novel aldolase isozymes in mouse sperm, two encoded by Aldoart

212 among fructose-1,6-(bis)phosphate aldolase (aldolase) isozymes.

213 Consistent with this hypothesis, aldolase knockdown cells show increased multinucleation.

214 However, aldolase knockdown does not affect glycolytic flux or in

215 One possible model for how aldolase knockdown may inhibit transformed cell prolifer

216 tococcus pyogenes, the tagatose bisphosphate aldolase LacD.1 likely originated through a gene duplica

217 Although a streptococcal aldolase, LacD.1, has been adapted to virulence gene reg

218 inding and photoactivation of labeled GAPDH, aldolase, lactate dehydrogenase, and pyruvate kinase rev

219 juvenile DM, creatinine phosphokinase level, aldolase level, absolute number of CD3-CD56+/16+ natural

220 as normal, as were serum creatine kinase and aldolase levels and thyroid, hepatic, and renal function

221 gests YfaU is instead a 2-keto-3-deoxy sugar aldolase like the homologous 2-dehydro-3-deoxygalactarat

222 ysis of such decarboxylations proceeds by an aldolase-like mechanism.

223 isomerase (MtnA), and an annotated class II aldolase-like protein (Ald2) to form 2-(methylthio)aceta

224 d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent

225 ochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization

226 catabolism is initiated by an intracellular aldolase/lyase mechanism.

227 nce of muscle edema in scleroderma, and that aldolase may be a useful biomarker to predict incident m

228 f cancer cell proliferation and suggest that aldolase may be a useful target in the treatment of canc

229 We have reported that the glycolytic enzyme aldolase mediates V-ATPase assembly and activity by phys

230 in folate biosynthesis, 7,8-dihydroneopterin aldolase (Mt-FolB), have C-terminal tails that could als

231 M.tuberculosis 7,8-dihydroneopterin aldolase (Mtb FolB, DHNA) is the second enzyme in the fo

232 that is unique to bacteria, dihydroneopterin aldolase (MtDHNA).

233 lucose, whereas the catalysis-defective D34S aldolase mutant, which still binds FBP, blocks AMPK acti

234 tive Thr deaminase in both tha1 and tha2 Thr aldolase mutants greatly increases seed Ile content, sug

235 Functional analysis of the aldolase mutants shows that disruption of binding betwee

236 In this study, we generate aldolase mutants that lack binding to the B subunit of V

237 by the combined action of transketolase and aldolase, of the seven-carbon bisphosphorylated sugar se

238 Among patients with aldolase or LDH levels detectable with a bead-based assa

239 ase (PdxA) oxidative decarboxylase, class II aldolase, or ribulose 1,5-bisphosphate carboxylase/oxyge

240 sphate and CO2 and (ii) the DUF1537/class II aldolase pair participates in pathways for the conversio

241 y for the presence of PfHRP2, pan-Plasmodium aldolase (pAldo), and pan-Plasmodium lactate dehydrogena

242 which simultaneously detects pan-Plasmodium aldolase (pAldo), pan-Plasmodium lactate dehydrogenase (

243 2.4-A and 2.7-A structures of P. falciparum aldolase (PfAldo) obtained from crystals grown in the pr

244 nal unidirectional fructose 1,6-bisphosphate aldolase/phosphatase, have been identified.

245 to glyceraldehyde-3-phosphate dehydrogenase, aldolase, phosphofructokinase, lactate dehydrogenase, an

246 omesin 3 and filamin-C), glycolytic enzymes (aldolase, phosphoglycerate mutase 2, beta enolase and gl

247 A sialate aldolase (pm1715) mutant unable to initiate dissimilatio

248 Pasteurella multocida sialic acid aldolase (PmAldolase), but not its Escherichia coli homo

249 itions 51% in the first turnover to form the aldolase products, 24% to the epimerase product and 25%

250 When unoccupied by FBP, aldolases promote the formation of a lysosomal complex c

251 th kinetic and structural studies on natural aldolases provides valuable feedback for computational e

252 Human V-ATPase directly interacts with aldolase, providing a coupling mechanism for glucose met

253 tory of the computationally designed (retro-)aldolase RA95.

254 Here we show that the artificial retro-aldolase RA95.5-8 is able to use a reactive lysine in a

255 The artificial aldolase RA95.5-8, for example, exploits amine catalysis

256 The aldolase reaction is strongly pH dependent, and apparent

257 rmediates (which would be generated from the aldolase reaction on each of these substrates) to move t

258 The aldolase reaction yields pyruvate, which supports growth

259 thermore, chemistry is rate limiting for the aldolase reaction, and the analysis of solvent kinetic i

260 ydroxyacetone phosphate and erythrose via an aldolase reaction.

261 cherichia coli) followed by an isomerase and aldolase sequentially function to salvage these two wast

262 The protein sequence of the evolved aldolase showed eight amino acid changes from the native

263 sight into the molecular determinants of FBP aldolase stereospecificity during aldol addition, a key

264 The AP-aldolase structure reveals the molecular basis of a here

265 y, the use of fluoropyruvate as a non-native aldolase substrate has arisen as a solution.

266 pFG is a bifunctional enzyme comprised of an aldolase subunit, DmpG, and a dehydrogenase subunit, Dmp

267 r than previous attempts to redesign natural aldolases, suggesting that such proteins may be excellen

268 is classified as a new member of the class I aldolase superfamily.

269 ed enzyme, E. coli tagatose-1,6-bisphosphate aldolase (TBPA), are described.

270 Moreover, native top-down ECD of aldolase tetramer reveals that ECD fragmentation is not

271 fitting of the isotopic distribution of the aldolase tetramer.

272 ssful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a diverge

273 ite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like pr

274 Whereas one Arabidopsis thaliana Thr aldolase (THA1) is expressed primarily in seeds and seed

275 yase (NAL, E.C. number 4.1.3.3) is a Class I aldolase that catalyzes the reversible aldol cleavage of

276 ne of the operon is nanL, which codes for an aldolase that cleaves NANA into N-acetyl mannosamine (ma

277 s for multistep reactions, we designed retro-aldolases that use four different catalytic motifs to ca

278 fuculose-1-phosphate by fuculose-1-phosphate aldolase, the MJ1418 gene product.

279 of calpain, Sm29, and fructose-bisphosphate aldolase, themselves potential vaccine antigens, suggest

280 s its cytoplasmic tail connects to actin via aldolase, thus driving parasite motility and host cell i

281 ar channel in the protein structure from the aldolase to the dehydrogenase active site.

282 ivity assays on the surfaces with DNA-linked aldolase to validate that, despite being modified with D

283 aldolases fructose-1,6-(bis)phosphate (FBP) aldolase, transaldolase, and 2-keto-3-deoxy-6-phosphoglu

284 e-site-directed mutagenesis to afford KDPGal aldolase variant NR8.276-2, which exhibits a 60-fold imp

285 could be rescued with an enzymatically dead aldolase variant that retains the known F-actin binding

286 on, where SNX9 binds near the active site of aldolase via residues 165-171 that are also required for

287 t L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase was created by directed evolution from the Esch

288 E. coli KDPGal aldolase was evolved using a combination of error-prone

289 ght into the function of different T. gondii aldolases, we first determined the crystal structures of

290 SFEC, pyruvate kinase and aldolase were co-localized by immunofluorescence to the

291 In cooked or roasted foods, enolase and aldolase were detectable in chicken breast while parvalb

292 isphosphatase, and fructose-1,6-bisphosphate aldolase were indicated.

293 an be used as a functional mimic of tagatose aldolase, whereas (R)-proline can be regarded as an orga

294 is required for the direct interaction with aldolase, whereas the second upstream acidic region is n

295 ractions with the abundant glycolytic enzyme aldolase, which also binds to the LC4 domain of SNX9.

296 ral metabolic function resides in the LacD.2 aldolase, which is required for the catabolism of galact

297 rease in glycolysis at the step catalyzed by aldolase, while activating PIK3CA mutations have the opp

298 ion of a 158 kDa protein complex, tetrameric aldolase with an average absolute deviation of 0.36 ppm

299 Here we show that an artificial aldolase with high specificity for acetone as the aldol

300 th AtLSMT-L and PsLSMT are able to methylate aldolases with similar kinetic parameters and product sp