ライフサイエンスコーパス: aleurone

1 lower expression in wild type relative to d1 aleurone.

2 egulate conversion of oil to sugar in barley aleurone.

3 subcellular localization of ferulate in the aleurone.

4 utant plants did not show a phenotype in the aleurone.

5 appearance of alpha-amylase released by the aleurone.

6 tivator of gene expression in the developing aleurone.

7 inhibitor of gibberellin signaling in barley aleurone.

8 roduction of very long chain omega-7s in the aleurone.

9 nin pathway exhibits strict cell autonomy in aleurone.

10 minished the expression of the Amy1A gene in aleurone.

11 asts of barley (Hordeum vulgare cv Himalaya) aleurone.

12 rall increase in flavonoid production in the aleurone.

13 al estimated from the total content of FA in aleurone.

14 e carotenoids and zeins and to differentiate aleurone.

15 ors showed localized patches of multilayered aleurone.

16 of these proteins also occurred in isolated aleurones after treatment with gibberellin, and this was

17 ence 6.4% of the transcriptome in developing aleurone and 6.7% in starchy endosperm.

18 eak alleles result in endosperms with mosaic aleurone and deformed plants with epidermal cells that r

19 rane preparations from oat (Avena sativa L.) aleurone and from leaves and stems of wild-type and GA-s

20 onding milling by-products (bran, middlings, aleurone and I, II and III steps of debranning) and flou

21 ctor ABI5, as assayed functionally in barley aleurone and physically in the yeast-two-hybrid assay.

22 cus, required for anthocyanin pigment in the aleurone and scutellum of the Zea mays (maize) seed, was

23 We show that both aleurone and starchy endosperm cells can be successfully

24 In contrast to aleurone and starchy endosperm cells, transfer cells fai

25 The aleurone and starchy endosperm share a common lineage th

26 ultured endosperms undergo normal cell type (aleurone and starchy endosperm) differentiation and stor

27 s system has been evaluated by analysing the aleurone and sub-aleurone cells of mature wheat grain, s

28 RNase) expressed in barley (Hordeum vulgare) aleurone and the gene for a second barley RNase expresse

29 with C1 for anthocyanin pigmentation in the aleurone and wx1 for amylose synthesis in the starchy en

30 lso contributes to omega-7 production in the aleurone, and aad3 aad2 exhibits an approximately 85% re

31 ad higher expression in wild type than in d1 aleurone, and genes down-regulated by GA had lower expre

32 tion of OsCBL2 and HvCBL2 in rice and barley aleurone because changes in cytosolic calcium have been

33 responding mRNA levels in the mature imbibed aleurone but are repressed 10-fold or more within 24 h o

34 enhanced the radical scavenging activity of aleurone by up to 4-fold, which overlapped the overall a

35 to horseradish peroxidase to screen a barley aleurone cDNA expression library for CaM binding protein

36 ase 1 and protein phosphatase 2A from a rice aleurone cDNA library.

37 , grain and grain cells (transfer cell (TC), aleurone cell (AL), and starchy endosperm (SE)).

38 bscisic acid (ABA) slows down the process of aleurone cell death and isolated aleurone protoplasts ca

39 Aleurone cell death does not follow the apoptotic pathwa

40 altered pattern of anthocyanin synthesis and aleurone cell differentiation in endosperm.

41 sts a hierarchy of gene functions to specify aleurone cell fate and then control aleurone differentia

42 onally, our data suggest that acquisition of aleurone cell fate does not solely rely upon signalling

43 URONE LAYER1 (SAL1), a negative regulator of aleurone cell fate encoding a class E vacuolar sorting p

44 ntrols a gene regulatory network involved in aleurone cell fate specification and cell differentiatio

45 These data suggest a model for aleurone cell fate specification in which DEK1 perceives

46 -specific fluorescence markers, we show that aleurone cell fate specification occurs exclusively in r

47 NKLY4 (CR4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma

48 k1) gene is a central regulator required for aleurone cell fate specification.

49 The starchy endosperm and aleurone cell fates are freely interchangeable throughou

50 teinase region (DEK1-CALP), is essential for aleurone cell formation at the surface of maize (Zea may

51 the role of vacuole acidification in cereal aleurone cell function.

52 The relationship between the aleurone cell integrity and the exposure or release of b

53 Vacuolation of the aleurone cell is a diagnostic feature of the response to

54 ndosperm transfer cell layer (BETL), and the aleurone cell layer (AL).

55 number of mRNA species in the mature imbibed aleurone cell of barley, such as a storage globulin, are

56 nd OsCBL3 were localized to the tonoplast of aleurone cell protein storage vacuoles and OsCBL4-green

57 have been implicated in the response of the aleurone cell to GA.

58 required for the ABA response of the cereal aleurone cell.

59 iggering the subsequent ABA responses of the aleurone cell.

60 Unlike the starchy endosperm, aleurone cells accumulate these storage proteins inside

61 Sporadic aleurone cells along the fusion plane were observed and

62 epressor, OsWRKY71 is localized to nuclei of aleurone cells and binds specifically to functionally de

63 s, with no difference being detected between aleurone cells and starchy endosperm cells.

64 Cereal aleurone cells are specialized endosperm cells that func

65 vacuole (PSV) becomes acidified rapidly when aleurone cells are treated with gibberellic acid (GA) bu

66 Unlike the cells of the starchy endosperm, aleurone cells are viable in mature grain but undergo PC

67 Mutants possess multiple layers of aleurone cells as well as aborted embryos.

68 Over-expression of HvWRKY38 in aleurone cells by particle bombardment blocks GA inducti

69 Barley aleurone cells contain three nucleases whose activities

70 lifespan of the endosperm, with internalized aleurone cells converting to starchy endosperm cells and

71 mation of apoptotic bodies do not occur when aleurone cells die.

72 p1-mediated repression of hydrolase genes in aleurone cells during seed development is determined by

73 of fluorescent dye tracers showed that young aleurone cells established symplastic subdomains through

74 or OsCBL2 in promoting vacuolation of barley aleurone cells following treatment with GA.

75 ) mutant line carrying up to seven layers of aleurone cells in defective kernel endosperm compared wi

76 sal1-2 endosperm has two to three layers of aleurone cells in normal, well filled grains.

77 The opening of aleurone cells increased the physical exposure of FA bou

78 PCD in barley aleurone cells is accompanied by the accumulation of a s

79 The expression of HvWRKY38 in barley aleurone cells is down-regulated by GA, but up-regulated

80 We conclude that cell death in aleurone cells is hormonally regulated and is the final

81 that GA-induced alpha-amylase production in aleurone cells is inhibited by bioactive SA, but not its

82 We tested the hypothesis that PCD in aleurone cells occurs by apoptosis, and show that the ha

83 d by these hormones were investigated in the aleurone cells of barley seeds using double-stranded RNA

84 The Vp1 promoter is active in the embryo and aleurone cells of developing seeds and, upon drought str

85 Constitutive expression of TaABF1 in aleurone cells of imbibing grains completely eliminated

86 evaluated by analysing the aleurone and sub-aleurone cells of mature wheat grain, showing high spati

87 In the aleurone cells of the cereal grain, gibberellic acid (GA

88 enzymes (principally alpha-amylases) in the aleurone cells of the endosperm, which then mobilize the

89 bombardment-mediated transient expression in aleurone cells represses the expression of two reporter

90 Arabidopsis aleurone cells responded to nitric oxide (NO), gibberell

91 Sensitivity to H2O2 in GA-treated aleurone cells results from a decreased ability to metab

92 Gain- and loss-of-function studies in barley aleurone cells show that HvABI5 expression is positively

93 Over-expression of these two genes in aleurone cells specifically and synergistically represse

94 y-GUS reporter gene in developing vp1 mutant aleurone cells strongly depends on the presence of a viv

95 signal transduction pathways exist in cereal aleurone cells that enable them to modulate hydrolase pr

96 ents occurring until late development caused aleurone cells to switch fate to starchy endosperm indic

97 The percentage of aleurone cells transformed following this method varied

98 ll death was studied in barley (cv Himalaya) aleurone cells treated with abscisic acid and gibberelli

99 ive and do not degrade their DNA, but living aleurone cells treated with GA accumulate nucleases and

100 Herein, we report transcriptomes of aleurone cells treated with the hormones abscisic acid,

101 Barley aleurone cells undergo programmed cell death (PCD) when

102 m cells remain competent to differentiate as aleurone cells until late in development.

103 1 and OsWRKY51 are revealed in the nuclei of aleurone cells using bimolecular fluorescence complement

104 ta-glucuronidase, and introduced into barley aleurone cells using the particle bombardment method.

105 ase reporter genes when introduced to barley aleurone cells via particle bombardment.

106 Arabidopsis aleurone cells were examined by light and electron micro

107 ination of the connated kernel revealed that aleurone cells were present for only a short distance al

108 d) rather than cyanidin (purple) pigments in aleurone cells where the anthocyanin biosynthetic pathwa

109 ave pleiotropic phenotypes including lack of aleurone cells, aborted embryos, carotenoid deficiency,

110 nsists of an epidermal-like surface layer of aleurone cells, an underlying body of starchy endosperm

111 ment of aleurone signaling molecules between aleurone cells, and SAL1 maintains the proper plasma mem

112 ansduction pathway were introduced into rice aleurone cells, and then the level of the OsCa-atpase tr

113 In a transient assay in rice aleurone cells, expression of the introduced Ca(2+)-ATPa

114 in regulating GAMYB-mediated GA signaling in aleurone cells, thereby establishing a novel mechanism f

115 y the components of transduction pathways in aleurone cells, we have investigated the effect of okada

116 homeostasis and autophagic flux in endosperm aleurone cells, where the ER accumulates lipid droplets

117 come positioned at the surface converting to aleurone cells.

118 -inducible and GA-repressible in embryos and aleurone cells.

119 transcriptional repressor of GA signaling in aleurone cells.

120 nd -72 are induced by abscisic acid (ABA) in aleurone cells.

121 d-induced HVA22 or HVA1 promoter activity in aleurone cells.

122 among which OsWRKY71 is highly expressed in aleurone cells.

123 utant allele contains two to three layers of aleurone cells.

124 apoptotic bodies, are not observed in dying aleurone cells.

125 s in death of GA-treated but not ABA-treated aleurone cells.

126 ROS in hormonally regulated death of barley aleurone cells.

127 nally regulated cell death pathway in barley aleurone cells.

128 of nuclease activities correlate with PCD in aleurone cells.

129 ed for the GA-dependent signaling pathway in aleurone cells.

130 lin (GA)-dependent signaling pathway in rice aleurone cells.

131 ot only in the starchy endosperm but also in aleurone cells.

132 oter regulate its expression level in barley aleurone cells.

133 ultrastructure was similar to that of cereal aleurone cells.

134 tially associated with plasmodesmata between aleurone cells.

135 The pale aleurone color1 (pac1) locus, required for anthocyanin p

136 ssue of mice that consumed rye bran or wheat aleurone compared with cellulose (P < 0.001).

137 However, aad3 ssi2 aleurone contained the same amount of omega-7s as aad3 W

138 Intake of rye bran and wheat aleurone decreases colonic serotonin in mice.

139 mainder of the fusion plane, suggesting that aleurone development is suppressed when positioned betwe

140 Excision of the Sho element early in aleurone development results in the characteristic "marb

141 A genetic analysis of maize aleurone development was conducted.

142 s, suggesting that cytokinin can also affect aleurone development.

143 as the testa and pericarp are important for aleurone development.

144 These mutants disrupt aleurone differentiation in reproducible patterns sugges

145 analysis of additional mutants that disrupt aleurone differentiation suggests a hierarchy of gene fu

146 specify aleurone cell fate and then control aleurone differentiation.

147 novel negative function in the regulation of aleurone differentiation.

148 ymes used for protoplast preparation degrade aleurone DNA and that DNA degradation by these nucleases

149 To analyze signaling between the embryo and aleurone during seed development, a T-B3La chromosome tr

150 stream sequence conferred the same levels of aleurone expression as nontransgenic B-Peru plants, but

151 al cues are required to specify and maintain aleurone fate and Dek1 function is required to respond t

152 ment indicating that positional cues specify aleurone fate.

153 Mutants in dek1 block aleurone formation at an early stage and cause periphera

154 In the seed, loss of cr4 inhibits aleurone formation in a pattern that reflects the normal

155 were hand dissected into endosperm, germ and aleurone fractions.

156 The cereal aleurone functions during germination by secreting hydro

157 iated cells that show sporadic expression of aleurone identity markers such as a viviparous1 promoter

158 c acid (FA) with the antioxidant capacity of aleurone in in vitro and under simulated gastric conditi

159 nucleus of both protonemal cells and barley aleurone, indicating that the nuclear localization signa

160 ng programmed cell death (PCD) in the cereal aleurone is described.

161 cPrG acts as a potential H+/Cl- symporter in aleurone is presented.

162 The aleurone is the outermost layer of cereal endosperm and

163 riched in palmitic acid, while the seed coat/aleurone layer accumulated vaccenic, linoleic, and alpha

164 present to different degrees in the maturing aleurone layer and embryo, but not in the starchy endosp

165 utermost cells, which differentiate into the aleurone layer and remain living in the mature seed.

166 measured, and these data show that both the aleurone layer and the embryo expressed the NO-associate

167 with whole seed compartments: the scutellar aleurone layer and the newly named endosperm adjacent to

168 general and emphasize the versatility of the aleurone layer as a model system for studying plant prot

169 istically to regulate gluconeogenesis in the aleurone layer as well as controlling the production and

170 The aleurone layer had zeaxanthin levels 2- to 5-fold higher

171 The cereal aleurone layer is a model system for studying the regula

172 The barley aleurone layer is a terminally differentiated secretory

173 The maize (Zea mays) aleurone layer occupies the single outermost layer of th

174 ted close correlation between embryo and the aleurone layer of endosperm.

175 ion in embryos, as well as the scutellum and aleurone layer of germinating seeds.

176 Development of the aleurone layer of maize grains requires the activity of

177 dlings, vascular tissue of nodes, embryo and aleurone layer of seeds, and young flowers.

178 b mRNA occurred in the starchy endosperm and aleurone layer of the developing seed, but not in the em

179 ost b alleles in that it is expressed in the aleurone layer of the seed.

180 ysis identified 73 glycosylation sites in 65 aleurone layer proteins, with 53 of the glycoproteins fo

181 er of starchy endosperm cells underneath the aleurone layer showed transformed cells.

182 ncreased Zn was broadly distributed from the aleurone layer to the inner endosperm.

183 The aleurone layer was found to be the primary determinant o

184 repressible mRNA persists in the mature dry aleurone layer, but is degraded during imbibition, reple

185 as normal maize (Zea mays [Zm]) has a single aleurone layer, naked endosperm (nkd) mutants produce mu

186 contrast, MT4 was confined to the embryo and aleurone layer, where it appeared during tissue speciali

187 Among those yet to be studied is the aleurone layer, which produces hydrolases for mobilizati

188 age oil is a major constituent in the cereal aleurone layer.

189 L gene, OsCBL2, is up-regulated by GA in the aleurone layer.

190 and in the amount of ions released from the aleurone layer.

191 sis and the endosperm is chalky and lacks an aleurone layer.

192 t to report on carotenoid composition of the aleurone layer.

193 ed; this gene and the gene encoding the seed aleurone-layer xylanase had strict tissue-specific expre

194 ces of many microbial xylanases and a barley aleurone-layer xylanase.

195 SUPERNUMERARY ALEURONE LAYER1 (SAL1), a negative regulator of aleurone

196 ransposon lines identified the supernumerary aleurone layers 1-1 (sal1-1) mutant line carrying up to

197 perm would affect the development of the two aleurone layers along the fusion plane.

198 the level of its mRNA readily detectable in aleurone layers and embryos, yet undetectable in the sta

199 show that cPrG prevents PSV acidification in aleurone layers and prevents synthesis of secretory prot

200 When barley (Hordeum vulgare) aleurone layers are subjected to heat shock there is a s

201 rotein secretion in gibberellic acid-induced aleurone layers by two independent mechanisms, heat shoc

202 of gene constructs were introduced to barley aleurone layers by using particle bombardment: the repor

203 Incubation of barley (Hordeum vulgare) aleurone layers in H2O2 causes rapid death of all cells

204 demonstrated that cycloheximide treatment of aleurone layers increased mRNA levels 4-fold, whereas a

205 Mutant kernels have several aleurone layers instead of one, indicating that Xcl1 alt

206 Barley (Hordeum vulgare) aleurone layers maintained in vitro respond to gibberell

207 eds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) we

208 suppression of genes has been determined in aleurone layers of barley seeds.

209 en germination is triggered or when isolated aleurone layers or protoplasts are incubated in gibberel

210 Incubation of aleurone layers or protoplasts in H(2)O(2)-containing me

211 revent DNA degradation during isolation from aleurone layers or protoplasts.

212 lecular changes that occurred in embryos and aleurone layers prior to germination were measured, and

213 fluorescent probe, Hordeum vulgare (barley) aleurone layers produce NO rapidly when nitrite is added

214 2 mRNA decline rapidly following exposure of aleurone layers to GA.

215 ide dismutase are strongly down-regulated in aleurone layers treated with GA.

216 RNA under anoxic conditions was decreased in aleurone layers while it increased in the embryo.

217 ugh cPrG blocks many GA-induced responses of aleurone layers, it does not affect early steps in GA si

218 In barley aleurone layers, the expression of genes encoding alpha-

219 d phytate is inhibited by cPrG in GA-treated aleurone layers.

220 ssion have been previously defined in barley aleurone layers.

221 on of GA-inducible gene expression in cereal aleurone layers.

222 at shock proteins, decreased in heat-shocked aleurone layers.

223 ntibody for detection and an antibody to the aleurone-localised 8S globulin as a control.

224 purple-pericarp sweetcorn (61.1%) and cherry-aleurone maize (74.6%).

225 e-pericarp sweetcorn (75.5% of TAC) and blue-aleurone maize (91.6%), while pelargonidin-based glucosi

226 Aleurone mosaicism was observed in the crown region of n

227 f in planta endosperm, including fidelity of aleurone mutant phenotypes, temporal and spatial control

228 We show that endogenous barley aleurone nucleases and nucleases present in enzymes used

229 Cell death in barley aleurone occurs only after cells become highly vacuolate

230 (omega-7s) are specifically enriched in the aleurone of Arabidopsis (Arabidopsis thaliana) seeds.

231 ated in pericarp layer of purple but only in aleurone of blue corn.

232 p101 mRNA accumulated in the subaleurone and aleurone of developing kernels and was highest in the ro

233 We also measured transcript abundance in aleurone of dwarf1 (d1) mutant rice.

234 seeds at early stages and in the embryo and aleurone of germinating seeds up to 24 h of imbibition.

235 Synthesis of the recombinant enzyme in the aleurone of germinating transgenic grain with an alpha-a

236 ious induction of alpha-amylase genes in the aleurone of the developing seed.

237 articularly in the leaf epidermis and in the aleurone of the endosperm.

238 Our findings suggest that the aleurone of wheat, oat, corn and germ of barley have sig

239 urces of fiber (rye bran flour, ground wheat aleurone, or powdered cellulose).

240 nucleases play a role in DNA cleavage during aleurone PCD.

241 n B-Bolivia that can account for the reduced aleurone pigment amounts (40%) observed with B-Bolivia r

242 of R-sc, r-m9 conditions a reduced level of aleurone pigmentation due to the presence of a 2.1-kb Ds

243 ment results in the characteristic "marbled" aleurone pigmentation pattern conferred by R-mb.

244 it to PLD activation are associated with the aleurone plasma membrane.

245 In barley aleurone, PpABI3A transactivates Em-GUS but to a lesser

246 ive oxygen species (ROS) are key elements in aleurone programmed cell death.

247 ther, these data demonstrate that GA-treated aleurone protoplasts are less able than ABA-treated prot

248 process of aleurone cell death and isolated aleurone protoplasts can be kept alive in media containi

249 Light microscopy showed that aleurone protoplasts contain two distinct types of vacuo

250 We report that the application of ABA to aleurone protoplasts increased the activity of the enzym

251 Aleurone protoplasts incubated in abscisic acid remained

252 at the pH of secondary vacuoles was lower in aleurone protoplasts incubated in gibberellic acid than

253 Application of pertussis toxin to intact aleurone protoplasts inhibited the ability of ABA to act

254 The application of PPA to aleurone protoplasts led to an ABA-like inhibition of al

255 nducible or otherwise was detected in barley aleurone protoplasts transfected with the PpLEA-1::GUS c

256 Illumination of barley aleurone protoplasts with blue or UV-A light results in

257 fluorescent protein was expressed in barley aleurone protoplasts, fluorescence accumulated in the pl

258 vitro in microsomal membranes prepared from aleurone protoplasts.

259 Aleurone PSVs contain zein-rich protein inclusions, a ma

260 embled in the ER, zeins are delivered to the aleurone PSVs in atypical prevacuolar compartments that

261 er wheat, spring tritordeum and barley (blue aleurone, purple pericarp, and yellow endosperm) from th

262 min with 0-20 L/h steam) were applied on the aleurone-rich flour to modify the technological properti

263 Novel aleurone-rich wheat milling fraction developed and produ

264 one regulation of expression of mRNA for the aleurone RNase revealed that, like the pattern for alpha

265 g Sc+ expression, including darkly pigmented aleurone, scutellum, coleoptile, and scutellar node [Scp

266 The role of DEK1 in aleurone signaling is discussed.

267 signal, CR4 promotes the lateral movement of aleurone signaling molecules between aleurone cells, and

268 e roles for the function of the sal1 gene in aleurone signaling, including a defect in endosome traff

269 One uses the promoters of genes with aleurone-specific expression during germination and the

270 Immediately upstream of the aleurone-specific promoter elements and in the opposite

271 Our findings that novel aleurone-specific promoter sequences of the B-Peru trans

272 ose through the translocation of an existing aleurone-specific promoter to the b locus.

273 tion assays in aleurone tissue localized the aleurone-specific promoter to the first 176 bases of the

274 f dek1 in the signaling system that controls aleurone specification and other aspects of endosperm de

275 n between a viviparous vp1 embryo and mutant aleurone suggesting that a quiescent embyro is a source

276 lar enhancement of Amy-GUS expression in the aleurone, suggesting that the quiescent embryo present i

277 In isolated aleurones, the levels of the corresponding mRNA increase

278 Transient transformation assays in aleurone tissue localized the aleurone-specific promoter

279 ium within 24 h of seed germination, but the aleurone tissue surrounding the starchy endosperm eventu

280 a-glucosidase cDNA clone derived from barley aleurone tissue was expressed in Pichia pastoris and Esc

281 In cereal aleurone tissue, GA induces and ABA suppresses the expre

282 ast development, alpha-amylase production in aleurone tissue, NO-dependent expression of defence-rela

283 anin pathway but is expressed exclusively in aleurone tissue.

284 opy indicated oil accumulation in OsACBP2-OE aleurone tissues.

285 starch spreads across the endosperm from the aleurone towards the crease.

286 Using a barley aleurone transient expression system, we have now locali

287 ption factor, VP1, on ABA action in a barley aleurone transient expression system.

288 imately 11,000 hybridized with RNA from rice aleurone treated with ABA, GA, or no hormone.

289 cDNA library constructed from mRNA of barley aleurones treated with gibberellin A 3 (GA).

290 lpha-amylase genes are expressed in wild oat aleurone, two genes, alpha-Amy2/A and alpha-Amy2/D, were

291 rized gene expression in rice (Oryza sativa) aleurone using a half-genome rice microarray.

292 d the V-type H(+)-ATPase present in isolated aleurone vacuoles.

293 The pattern of aleurone variegation of maize kernels carrying Ac and bz

294 alls of the starchy endosperm but not in the aleurone walls.

295 e improvement in the antioxidant capacity of aleurone was also observed in the simulated gastric dige

296 The antioxidant capacity of aleurone was increased by around 2-fold when its median

297 oligosaccharides (AXOS) obtained from wheat aleurone was investigated.

298 und that AAD3 expression is localized to the aleurone where mutants show an approximately 50% reducti

299 a+ and K+ within the phytate granules of the aleurone, with CN- being diagnostic for proteins and C(2

300 ied in the omega-7 content of Brassica napus aleurone, with the highest level detected being approxim