ライフサイエンスコーパス: allospecific

1 1 and Th2 cytokine network and systemic host allospecific Ab (allo-Ab) responses in the development o

2 notypically hostile NK cells that express an allospecific activating receptor without coexpressing an

3 vitro study quantified CD103+ T cells after allospecific activation with and without exogenous TGFbe

4 e findings highlight surprisingly asymmetric allospecific alterations in iNKT cells as they develop a

5 ernutrition and hypoleptinemia impact T-cell allospecific and cytomegalovirus (CMV) viral-specific im

6 not be of donor origin, suggesting that both allospecific and non-allospecific mechanisms regulate en

7 freshly prepared primary MLRs, to determine allospecific and nonspecific inhibitory and Treg recruit

8 to investigate how immunosuppression affects allospecific and pathogen-specific memory B cells, and r

9 ated hypoleptinemia correlated with impaired allospecific and viral-specific immunities.

10 mulated with HLA class I antibody (W6/32) or allospecific antibodies from sensitized patients (n=6) w

11 Importantly, allospecific antibodies from sensitized patients also ac

12 f this study was to test the contribution of allospecific antibody-secreting cells (ASCs) from differ

13 (r=0.682, P=0.005) and inversely with IR of allospecific, antiinflammatory, CTLA4(+)Tc-M (r=-0.638,

14 Suppression was allospecific, as Treg cells induced by third-party antig

15 e inability of the direct pathway to provide allospecific B cell help.

16 alloantigen, processed and presented by the allospecific B cell.

17 alloantibody responses, with splenic GCs and allospecific bone marrow plasma cells readily detectable

18 estigated the development and maintenance of allospecific CD103 T cells within the tubular microenvir

19 sayed for allospecific CD154CD19 B cells and allospecific CD154 TcM in 16-hr live-cell mixed leukocyt

20 ed significantly with IR of proinflammatory, allospecific CD154(+)Tc-M (r=0.682, P=0.005) and inverse

21 Allospecific CD154+T-cytotoxic memory cells (CD154+TcM)

22 ated monocytes correlated significantly with allospecific CD154+T-cytotoxic memory cells (Spearman r=

23 ng rATG concentrations, proportionately more allospecific CD154+T-cytotoxic memory cells (TcM) surviv

24 ic analyses, the rejection-risk threshold of allospecific CD154+T-cytotoxic memory cells (TcMs) assoc

25 Allospecific CD154+T-cytotoxic memory cells predict acut

26 Allospecific CD154+TcM correlated inversely with CTLA4+T

27 The strong association between ACR and allospecific CD154+TcM may be useful in minimizing proto

28 han TcM, resulting in relative enrichment of allospecific CD154+TcM.

29 Samples were assayed for allospecific CD154CD19 B cells and allospecific CD154 Tc

30 Allospecific CD154CD19 B cells identify rejection-prone

31 The IR of allospecific CD154CD19 B cells more than or equal to 1.3

32 d to the semi-direct pathway, as measured by allospecific CD4 (indirect) and CD8 T-cell clones (direc

33 attractants necessary for the recruitment of allospecific CD4 T cells into the graft.

34 Activation and cytokine secretion by allospecific CD4+ and CD8+ T cells were initially blocke

35 fer model of TCR transgenic T cells to track allospecific CD4+ T cell expansion and trafficking chara

36 Type 2 immune deviation of allospecific CD4+ T cells resulted in IL-4 and IL-5 prod

37 escribed factors affecting the generation of allospecific CD4CD25 forkhead/winged helix transcription

38 Further studies demonstrated that these allospecific CD4high cells were also present (< or = 1%

39 ergized to promote expansion and survival of allospecific CD8 CD103 T cells in vitro, but IL-15 down-

40 ions can strongly stimulate both NK cell and allospecific CD8 T cell responses, and these same effect

41 These results suggest that activated, allospecific CD8 T cells are recruited to tubules during

42 and adhesion molecules upon interaction with allospecific CD8 T suppressor cells or exposure to inhib

43 as the cytotoxic effector mechanisms used by allospecific CD8(+) cytolytic T cells.

44 The magnitude of allospecific CD8(+) T cell in vivo cytotoxic effector fu

45 Allospecific CD8(+) T lymphocytes are an important compo

46 -1 reduced cytokine-induced proliferation of allospecific CD8+ cloned L3 T cells and OVA-reactive CD4

47 cytokine requirements for the generation of allospecific CD8+ CTL in vitro and have found that IL-4

48 he development, function, and persistence of allospecific CD8+ T cell effectors in vivo.

49 In this study, we tested the role of IL-2 in allospecific CD8+ T cell memory by analyzing the long-te

50 endent inhibitory effect on proliferation of allospecific CD8+ T cells in mixed lymphocyte culture.

51 Adoptive transfer of allospecific CD8+ T cells purified 14 days postburn resu

52 entical type 2 cytokine-inducing conditions, allospecific CD8+ T cells were primed to become IL-4, IL

53 We asked whether allospecific CD8+ T cells, in the absence of CD4+ T cell

54 Allospecific cell lines generated from GNLY transgenic a

55 Allospecific cell lines were preincubated with anti-alph

56 95-ligand pathway; therefore the deletion of allospecific cells by donor spleen cells may be induced

57 1 partially protects HUVECs against lysis by allospecific class I MHC-restricted cytolytic T lymphocy

58 he differentiation of purified CD8+ PBL into allospecific, class I MHC-restricted CTL that lyse EC, b

59 PCR assays to monitor the levels of putative allospecific clonotypes in posttransplant blood samples

60 tosis by sHLA was analyzed by adding sHLA to allospecific CTL 4 or for 24 hr before flow cytometric a

61 This rapid generation of allospecific CTL activity during a viral infection prece

62 ime course studies showed that DSBM impaired allospecific CTL activity whether given on day -10 (3.3%

63 tion, they show that both CD4(+)- and CD8(+)-allospecific CTL can be isolated from rejected allogenic

64 Allospecific CTL can function as cellular effectors of s

65 ells, implying that soluble antigen promotes allospecific CTL death.

66 Thus, (1) DSBM inhibits allospecific CTL development in the allograft, (2) decre

67 nto F(1) hosts stimulates the development of allospecific CTL effectors that eliminate host lymphocyt

68 By providing exogenous cytokines to allospecific CTL generation cultures, we further demonst

69 neal cells produced a profound inhibition of allospecific CTL, DTH, and mixed-lymphocyte responses.

70 ells produces a dose-dependent inhibition of allospecific CTL, DTH, and mixed-lymphocyte responses.

71 costimulation blockade are able to generate allospecific CTL- and IFN-gamma-producing T cells within

72 regulate differentiation and/or expansion of allospecific CTL.

73 of CD8+, but anti-CD8-resistant, MHC class I-allospecific CTL.

74 s, providing evidence that the generation of allospecific CTLs during acute LCMV infection is antigen

75 ed, cardiac allografts and the generation of allospecific CTLs were not impaired in the absence of IL

76 ent donor cells differentiated normally into allospecific CTLs.

77 yme-linked immunospot assay for detection of allospecific cytokines produced by individual human PBLs

78 roliferative responses and the generation of allospecific cytolytic effectors.

79 and engage MHC class I antigens, leading to allospecific cytolytic responses and graft rejection.

80 HC-SMA were assessed for the development of allospecific cytolytic T cells (allo-CTLs).

81 BM infusion significantly reduced intragraft allospecific cytolytic T-cell (CTL) activity compared wi

82 alloantibody levels correlated with in vivo allospecific cytotoxic activity in CD8 knockout hepatocy

83 Moreover, the generation of allospecific cytotoxic activity is inhibited by IL-10.

84 ng the 19 clones analyzed: 1) TCR alphabeta+ allospecific cytotoxic cells, 2) TCR alphabeta+ nonspeci

85 cific cytotoxic cells, and 5) TCR alphabeta- allospecific cytotoxic cells.

86 mulated the proliferation and development of allospecific cytotoxic effectors in vitro and in vivo.

87 The demonstration of cloned, TCR alphabeta+, allospecific cytotoxic effectors provides the strongest

88 Administering IL-18 significantly enhanced allospecific cytotoxic function and expansion of CD8(+)

89 Moreover, mdr1a-/- T cells produced strong allospecific cytotoxic responses comparable to those of

90 alized splenic T lymphocyte subsets, reduced allospecific cytotoxic T lymphocyte activity, and increa

91 PG27 reduced day 7 splenic allospecific cytotoxic T lymphocyte levels by more than

92 from control rats exhibited greater than 35% allospecific cytotoxicity 8 days after grafting.

93 Here, we demonstrate that robust allospecific cytotoxicity is also manifest in vivo.

94 The development of in vivo allospecific cytotoxicity was determined by clearance of

95 reased nuclear NFkappaB in T cells and their allospecific cytotoxicity.

96 of these CD4+ T cell clones displayed strong allospecific cytotoxicity.

97 injected s.c. into naive recipients induced allospecific delayed hypersensitivity and elicited delay

98 Graft rejection, induction of allospecific delayed-type hypersensitivity (DTH) respons

99 Allospecific delayed-type hypersensitivity (DTH) was eva

100 Graft survival and allospecific delayed-type hypersensitivity (DTH) were us

101 ition to graft survival, graft infiltration, allospecific delayed-type hypersensitivity (DTH), and cy

102 raft rejection and prevent the generation of allospecific delayed-type hypersensitivity (DTH).

103 Furthermore, allospecific delayed-type hypersensitivity and gene expr

104 Additionally, allospecific delayed-type hypersensitivity was compared

105 ally, all CLN(-) hosts failed to demonstrate allospecific DTH (P < 0.001).

106 -1-/- mice receiving B10.D2 grafts developed allospecific DTH.

107 d untreated WT mice, as was the induction of allospecific DTH.

108 de future investigations of prenatal NK cell-allospecific education.

109 T cells as helper cells in the generation of allospecific effector macrophages in corneal graft rejec

110 ransgenic model, blockade of TIM-3 increased allospecific effector T cells, enhanced Th1 and Th17 pol

111 , to assess the capacity of TRegs to mediate allospecific effects.

112 However, DNA fragmentation induced by either allospecific FasL-defective CTL, or by perforin-deficien

113 ce from animal studies has demonstrated that allospecific FoxP3(+)CD4(+) regulatory T (Treg) cells ex

114 Allospecific GVL reactivity was reduced but not eliminat

115 results indicate the importance of adequate allospecific helper as well as effector T cells for the

116 igen, we identified and cloned a TCR from an allospecific HLA-A2-restricted, HCV:NS3:1406-1415-reacti

117 We therefore conclude that allospecific human CD8+ T cells of Tc1 and Tc2 phenotype

118 accompanied by markedly increased numbers of allospecific, IFN-gamma-producing cells in the periphery

119 In contrast, allospecific IgM and IgG were significantly decreased in

120 In contrast, there was no impairment of the allospecific IgM response.

121 lockade, there was a diminished frequency of allospecific IL-10-producing cells and an increased freq

122 proved ability to define the strength of the allospecific immune response by enzyme-linked immunospot

123 Nevertheless, an allospecific immune response could occur as a consequenc

124 by HFRPE cells may not induce a significant allospecific immune response.

125 vent posttransplant cytomegalovirus, inhibit allospecific immunity, and reduce tissue injury are need

126 Though T cells clearly mediate allospecific immunity, the manner in which reperfusion e

127 the anti-CD154-treated mice did not exhibit allospecific immunity.

128 atibility complex disparate skin grafts, the allospecific immunoglobulin (Ig)G response was markedly

129 activating receptor without coexpressing any allospecific inhibitory receptors.

130 skin transplantation, increased induction of allospecific iTregs and a reduction in T effector respon

131 The CD8(+) Ly49G2(+) population mediated no allospecific killing, nor was any NK-like killing observ

132 CAR Treg are providing hope in establishing allospecific, localized immune tolerance in the long ter

133 t the CD103+ and CD103- subsets both possess allospecific lytic activity.

134 e and prolong islet allograft survival in an allospecific manner.

135 n, suggesting that both allospecific and non-allospecific mechanisms regulate engraftment.

136 itro analyses revealed that the apoptosis of allospecific memory CD8(+) T cells is significantly incr

137 umin antigen, B cell depletion also impaired allospecific memory T cell responses and thereby enhance

138 Transgenic T cells were used to track allospecific memory T-cell generation.

139 We have previously shown that allospecific murine CD8+ T cells of the Tc1 and Tc2 phen

140 nswer this question, we investigated whether allospecific naive or memory T cells can mediate acute c

141 Tregs rather than prevention of expansion of allospecific natural Tregs.

142 , cell-extrinsic signals appeared to dictate allospecific patterns of Ly49 receptor expression and li

143 -restricted T cell hybridoma (B4.2.3), or an allospecific, peptide-dependent, T cell hybridoma (3DT52

144 tic function, and clonally deleted host-type allospecific precursor CTL in vitro.

145 in both CD4(+) and CD8(+) T cells, including allospecific proliferation and cytokine secretion.

146 0-producing CD8 T cells strongly inhibit the allospecific proliferation of naive CD8 T cells to monoc

147 y, fully mismatched graft were performed and allospecific proliferation was measured after depletion

148 ctions, so as to immunologically monitor the allospecific regulation after transplantation.

149 These results describe a novel allospecific regulatory CD8(+)PD1(+) T cell induced by I

150 is assay can thereby be helpful in assessing allospecific regulatory effects of diverse immunosuppres

151 oughout the overall T-cell repertoire, one's allospecific repertoire is similarly made up of cells in

152 man cells and tested agents known to inhibit allospecific responses for their ability to inhibit xeno

153 ess whether such a construct could affect Dd allospecific responses in vitro and in vivo.

154 grafts, the CD8+ Vbeta8+ cells demonstrated allospecific responses that were numerically larger than

155 d acute lung allograft rejection and reduced allospecific responsiveness by markedly decreasing monon

156 ation in the settings of both polyclonal and allospecific stimulation.

157 Cultured Tregs produced allospecific suppression, maintained demethylation of th

158 this drug interfere with the persistence of allospecific suppressor cells for 35 days after the graf

159 Similar to allospecific suppressors, these xenospecific suppressor

160 When combined with allospecific T and B cells, this information may differe

161 )) phenotype and were capable of suppressing allospecific T cell proliferation and IFN-gamma producti

162 eks following rejection, however, the memory allospecific T cell response became predominant in the r

163 of C5aR in both graft and recipient reduced allospecific T cell responses and prolonged renal allogr

164 T cells modulates their function, enhancing allospecific T cell responses that lead to allograft rej

165 n and subsequently enhances its capacity for allospecific T cell stimulation.

166 llo-Ab responses and induction of peripheral allospecific T cell unresponsiveness both in vitro and i

167 g of donor Th2 cell therapy, host-anti-donor allospecific T cells acquired Th2 polarity, persisted po

168 Indirect allospecific T cells activated by such CD11c(+) dendriti

169 olecules and were less potent at stimulating allospecific T cells after an additional 48-hour culture

170 iminished frequencies of IFN-gamma-producing allospecific T cells and reduced CTL activity.

171 tible with either deletion or suppression of allospecific T cells as possible mechanisms of tolerance

172 the DLN whether it contains naive or memory allospecific T cells as shown in experiments in which re

173 alysis of the phenotype and proliferation of allospecific T cells expanded in vitro in the presence o

174 It has been speculated that allospecific T cells express high-affinity T cell recept

175 directed to tissue antigens but differ from allospecific T cells in several important respects, refl

176 eneic bone marrow cells on the activation of allospecific T cells in vitro.

177 In contrast, direct allospecific T cells interacting with intact donor ECDI-

178 ining demonstrated that early recruitment of allospecific T cells into allografts around POD10 correl

179 ascularization and that early recruitment of allospecific T cells into the grafts promotes destructio

180 are important targets of alloantibodies and allospecific T cells involved in graft rejection.

181 esults uncover a functional heterogeneity in allospecific T cells of distinct specificities after tol

182 cells and/or soluble MHC Ags suppresses NIMA-allospecific T cells of the offspring, predisposing to o

183 ively, these results suggest that peripheral allospecific T cells undergo the initial stages of activ

184 These innate immune cells, in addition to allospecific T cells, can damage cardiomyocytes directly

185 ity in the level of dysfunction of different allospecific T cells, depending on duration of their cog

186 To target indirect allospecific T cells, ECDI-SPs induce upregulation of ne

187 y to defunctionalize a greater repertoire of allospecific T cells, thereby mitigating a critical vuln

188 their ability to act as stimulatory APCs for allospecific T cells.

189 sequently have reduced capacity to stimulate allospecific T cells.

190 ompromised capacity of the cells to activate allospecific T cells.

191 Allospecific T effector cells were induced in all mice u

192 llorecognition is responsible for generating allospecific T follicular helper cells remains unclear.

193 Moreover, allospecific T regulatory (Tr) cells correlated with acc

194 ivity acquired by dendritic cells exposed to allospecific T suppressor (Ts) cells.

195 We analyzed individual allospecific T-cell clones derived from a Wiskott-Aldric

196 CMV characteristically induces an anti-H2(d) allospecific T-cell response that includes T-cell clones

197 T-cell costimulatory blocking agents inhibit allospecific T-cell responses in vitro and prevent allog

198 cooperation, CD154 has been used to identify allospecific T-cytotoxic memory cells (TcM) for rejectio

199 These T cells are analogous to the "allospecific" T cells that have been described in hemato

200 R alphabeta+ nonspecific cytotoxic cells, 3) allospecific TCR alphabeta+ noncytotoxic cells, 4) TCR a

201 epends on the ability to block generation of allospecific Th1 responses that lead to rejection.

202 ) infected with influenza A virus have lower allospecific Th1-cell stimulatory abilities than DCs act

203 s also developed vigorous primary and memory allospecific Th1/Th2 responses that exceeded the respons

204 , because neonatal antigen exposure triggers allospecific Th2 CD4 memory cells, whereas antigen expos

205 her show that LCMV prevents the induction of allospecific tolerance and mixed hematopoietic chimerism

206 es, without CD40-CD40L costimulation, induce allospecific tolerance but may trigger allo-independent

207 ade is most effective at inducing long-lived allospecific tolerance if anergy and regulation can be e

208 ese findings illustrate the robust nature of allospecific tolerance in prenatal mixed chimerism compa

209 Moreover, it is uncertain whether any allospecific tolerance occurring with CD40-CD40L blockad

210 CD40L-/- transplant recipients developed allospecific tolerance to the donor haplotype; second se

211 as an efficacious cellular therapy to induce allospecific tolerance to transplantation antigens.

212 mphocytic choriomeningitis virus on prenatal allospecific tolerance was examined.

213 to induce mixed hematopoietic chimerism and allospecific tolerance.

214 tive NKT cells are required for induction of allospecific Tr cells and are essential for survival of

215 B/c mice, and the adoptive transfer of these allospecific Tr cells to Jalpha281 KO mice allowed a 50%

216 Adoptive transfer of allospecific transgenic CD4 T cells revealed a "split to

217 MMc) has been associated with development of allospecific transplant tolerance, antitumor immunity, a

218 established for selective expansion of human allospecific Treg cells ex vivo.

219 pamycin inhibitors have disparate effects on allospecific Treg generation using the Treg MLR.

220 erentially enhances the proliferation of the allospecific Tregs adoptively transferred in an antigen-

221 s has demonstrated that adoptive transfer of allospecific Tregs offers greater protection from graft

222 n a decreased frequency of overall number of allospecific Tregs.

223 ments, and function of alloantigen-specific (allospecific) Tregs from human blood.

224 kin resulted in graft rejection, with stable allospecific type 2 cytokine production in vivo.