ライフサイエンスコーパス: alpha granule

1 ting that TFPI is not stored within platelet alpha granules.

2 ng FV is found in plasma and within platelet alpha granules.

3 nhibited secretion of dense granules but not alpha granules.

4 , a basic protein found normally in platelet alpha granules.

5 (TS1), the most abundant protein of platelet alpha granules.

6 cytoplasmic membranes and reduced numbers of alpha granules.

7 the trans-Golgi network, late endosomes and alpha-granules.

8 s characterized by large platelets that lack alpha-granules.

9 ion requires release of stored proteins from alpha-granules.

10 odies packed with respiring mitochondria and alpha-granules.

11 n platelets and megakaryocytes and stored in alpha-granules.

12 re demonstrated, as was its association with alpha-granules.

13 delivered both in the plasma and in platelet alpha-granules.

14 rs and inhibitors into separate and distinct alpha-granules.

15 along with CD62P, into both MK and platelet alpha-granules.

16 VIII can be expressed and stored in platelet alpha-granules.

17 is accompanied by uptake of fibrinogen into alpha-granules.

18 secretion of plasma fibrinogen from platelet alpha-granules.

19 inogen) and then package these proteins into alpha-granules.

20 bitor, a trace protein contained in platelet alpha-granules.

21 been immunized with purified human platelet alpha-granules.

22 in a rapidly releasable form within platelet alpha-granules.

23 leads to degradation of most proteins of the alpha-granules.

24 diathesis and a marked reduction in platelet alpha-granules.

25 cytoplasm and Dock7 on the membrane of or in alpha-granules.

26 acrothrombocytopenia, with platelets lacking alpha-granules.

27 in that traffics FVIII more effectively into alpha-granules.

28 PS), a rare recessive platelet disorder with alpha-granule abnormalities and mutations in NBEAL2.

29 t a marked defect in exocytosis of delta and alpha granules, alphaIIbbeta3 activation, actin assembly

30 dense granule secretion; and (3) diminished alpha granule and lysosome secretion might contribute to

31 n microscopy, we confirmed that CD154 was an alpha granule and not a cell surface protein, and therea

32 P-1 helps to sequester TGF-beta1 in platelet alpha granules and activates TGF-beta1 once both protein

33 hibited platelet secretion of both dense and alpha granules and the secretion-dependent platelet aggr

34 caused increased platelet CD62P expression, alpha-granule and dense granule release, and the classic

35 tion, integrin alphaIIbbeta3 activation, and alpha-granule and dense granule secretion in response to

36 oprotein IIb or in embryos in which platelet alpha-granule and dense granule secretion is abolished.

37 elet exocytosis and differentially regulates alpha-granule and dense granule secretion.

38 hat mimic NSF sequence motifs inhibited both alpha-granule and dense-granule secretion in permeabiliz

39 ine signal to potentiate platelet release of alpha-granule and lysosome cargo and protein disulfide i

40 Ehrlich described the features of the alpha-granule and the cell's distribution in various spe

41 von Willebrand factor (VWF) is stored within alpha-granules and accounts for approximately 20% of tot

42 These platelets have few alpha-granules and an increased number of lysosomal-like

43 es can enable the storage of FIX in platelet alpha-granules and corrects the murine hemophilia B phen

44 a patient with gray platelet syndrome lacked alpha-granules and demonstrated only minimal spreading.

45 llagen were able to secrete contents of both alpha-granules and dense granules and generate thromboxa

46 to establish how cargos are segregated among alpha-granules and determine whether or not different al

47 n; (2) significant decreases of secretion of alpha-granules and GPIIb-IIIa activation induced by aden

48 ranules was completely ablated and that from alpha-granules and lysosomes was severely compromised.

49 2alpha inactivation have critical defects in alpha-granules and membrane structure that are associate

50 disorder characterized by platelets lacking alpha-granules and progressive marrow fibrosis.

51 in high concentrations of TGF-beta1 in their alpha-granules and release it on platelet adhesion/activ

52 ne megakaryocytes, it was stored in platelet alpha-granules and released at sites of injury.

53 oagulation factor V (FV) from the media into alpha-granules and releases functional FV(+)CD42b(+) hum

54 FIX can be expressed and stored in platelet alpha-granules and that platelet-derived FIX can correct

55 rombocytopenia, and a deficiency of platelet alpha-granules and their cargo, including von Willebrand

56 developing megakaryocytes would be stored in alpha-granules and whether its release from circulating

57 quired for release from dense core granules, alpha granules, and lysosomes.

58 ribution of the demarcation membrane system, alpha-granules, and other cytoplasmic organelles.

59 gray platelet syndrome platelets, which lack alpha-granules, and Wiskott-Aldrich syndrome platelets,

60 Proteins stored in alpha-granules are also thought to play a role in inflam

61 alpha-granules are by far the most abundant platelet gra

62 These observations suggest that the alpha-granules are correctly assembled but the contents

63 uring spreading, supporting the premise that alpha-granules are heterogeneous and demonstrating that

64 arrow megakaryocytes, which store FBG in the alpha-granules, are thought not to express the FBG genes

65 S stimulates platelet secretion of dense and alpha granules as indicated by ATP release and P-selecti

66 eptolysin O-permeabilized platelets released alpha-granules, as measured by surface expression of P-s

67 e defect, demonstrating a role for VPS16B in alpha-granule biogenesis and taking us one step closer t

68 characteristics of human GPS, with defective alpha-granule biogenesis in MKs and their absence from p

69 Sec1/Munc18 protein VPS33B are required for alpha-granule biogenesis.

70 lta-granule development, less is known about alpha-granule biogenesis.

71 regulator of the genes required for platelet alpha-granule biogenesis.

72 ng the COMMD3/CCC complex as a new factor in alpha-granule biogenesis.

73 (T2), and studied the platelet phenotype and alpha-granule biogenesis.

74 ndently of VPS33B/VPS16B at a later stage of alpha-granule biogenesis.

75 is essential for megakaryocyte and platelet alpha-granule biogenesis.

76 PF4 is stored in platelet alpha-granules bound to the glycosaminoglycan (GAG) chai

77 1 indicate that TLT-1 is not simply cargo of alpha-granules but may instead regulate granule construc

78 telet factor 4 (PF4) is an abundant platelet alpha-granule C-X-C chemokine that has weak chemotactic

79 It did not occur in mice lacking alpha-granules, C type lectin receptor-2 (CLEC-2), or pr

80 used to quantify the release of 28 distinct alpha-granule cargo molecules.

81 w that platelets can orient their release of alpha-granule cargo to promote spreading beyond the dot'

82 in and collagen increased exosome content of alpha-granule chemokines CXCL4 and CXCL7 and cytoplasmic

83 This study demonstrates that platelet alpha-granule constituents are critically required not o

84 ting that both releasable and membrane-bound alpha-granule constituents were absent.

85 Platelet alpha-granules constitute the major rapidly releasable r

86 s on liver regeneration are unclear, because alpha-granules contain bioactive molecules with opposing

87 platelet alpha-granules, unusual stimulated alpha granule content release, abnormal lipid inclusions

88 ted that platelets selectively release their alpha-granule content dependent on the environmental sti

89 studies have demonstrated a process by which alpha-granule contents are released from platelets.

90 latelets even though all dense core and most alpha-granule contents had been released.

91 pha-granule exocytosis and deposition of the alpha-granule contents such as fibrinogen and fibronecti

92 He speculated correctly that the alpha-granule contents were secretory products and descr

93 ith syntaxin-4 and promoted the secretion of alpha-granule contents, including TGF-beta and CCL5.

94 sponsible for efficient release of dense and alpha-granule contents; thus, VAMP-8(-/-) mice are a use

95 the hypothesis that distinct populations of alpha-granules could undergo selective release.

96 ) platelets identified a marked reduction in alpha-granule count and the presence of small granule-li

97 In addition, a platelet/megakaryocyte alpha granule defect, similar to that of patients with g

98 platelets from patients with an inheritable alpha-granule defect, demonstrating a role for VPS16B in

99 ult of the characterization of patients with alpha-granule deficiencies.

100 In addition, we demonstrate that alpha-granule deficiency impairs platelet function beyon

101 irment in MVB maturation in MKs underlie the alpha-granule deficiency in Vps33b(fl/fl)-ER(T2) mouse a

102 for aggregation, and gray platelet syndrome alpha-granule-deficient platelets do not aggregate to 4

103 er associated with macrothrombocytopenia and alpha-granule-deficient platelets.

104 We previously showed that platelet (PLT) alpha granule-delivered urokinase plasminogen activator

105 oG(-/-) platelets was reduced secretion from alpha-granules, dense granules, and lysosomes following

106 We measured plasma levels of alpha-granule-derived factors in the liver vein at the e

107 n combination with the loss of proteins from alpha-granules drives the development of bone marrow fib

108 Thus, release of thrombospondin-1 from alpha-granules during activation provides positive feedb

109 )](i), but it is specifically coupled to the alpha-granule exocytic pathway without concomitant effec

110 alpha Granule exocytosis from VAMP-7(-/-) platelets was

111 Interestingly, alpha-granule exocytosis and deposition of the alpha-gra

112 Time-lapse microscopy demonstrated that alpha-granules expressing VAMP-7 actively moved from the

113 In contrast, alpha-granules expressing VAMP-7 translocated to the per

114 may be caused by an abnormality in platelet alpha-granule factor V distinct from that described for

115 ith collagen and thrombin express functional alpha-granule factor V only on a subpopulation of cells.

116 vesicle trafficking pathways responsible for alpha-granule formation are beginning to be assembled as

117 nction, and they also indicate that abnormal alpha-granule formation in Nbeal2(-/-) mice has deleteri

118 les with increased size as compared with the alpha-granules from control mouse platelets.

119 s of dense granules, lysosomal granules, and alpha-granules from human platelets by S-nitrosylation o

120 pha-granule secretion that include homotypic alpha-granule fusion and the fusion of alpha-granules wi

121 -FV platelets may explain the unique role of alpha-granule FV and the hemostatic effectiveness of you

122 s express very high levels of surface-bound, alpha-granule FV on simultaneous activation with 2 agoni

123 regulates not only megakaryopoiesis but also alpha-granule generation and signaling pathways required

124 Factor V (FV) present in platelet alpha-granules has a significant but incompletely unders

125 escribe cells with granules (which he called alpha-granules) having an affinity for eosin and other a

126 telet factor 4 (PF4) is an abundant platelet alpha-granule heparin-binding protein.

127 ltimate secretion; (2) impaired secretion of alpha granules in HPS, and to some degree of lysosomes,

128 on microscopy revealed a reduced quantity of alpha granules in normally sized platelets.

129 leeding diathesis characterized by a lack of alpha granules in platelets.

130 servoirs within the vascular endothelium and alpha granules in platelets.

131 bleeding disorder caused by a deficiency of alpha granules in platelets.

132 atients' fibroblasts and by the depletion of alpha granules in their platelets.

133 duced platelet aggregation; and large, fused alpha-granules in 1% to 5% of circulating platelets.

134 and morphologically distinct populations of alpha-granules in megakaryocytes and platelets may provi

135 rombocytopenia, splenomegaly, and paucity of alpha-granules in megakaryocytes and platelets.

136 Fourth, an unanticipated role for alpha-granules in platelet spreading has been identified

137 are separated in distinct subpopulations of alpha-granules in platelets and megakaryocytes.

138 rized by thrombocytopenia and the absence of alpha-granules in platelets.

139 REM-like transcript-1, which is localized to alpha-granules in resting platelets, binds fibrinogen, a

140 in protein, SEPT5, to the presence of larger alpha-granules in the GP Ibbeta(Null) platelet.

141 ed that platelet FVIII is stored in platelet alpha-granules independent of the presence of VWF.

142 is and elaboration of TGF-beta from platelet alpha-granules into the circulation.

143 y antagonistic functions are packaged within alpha-granules is controversial.

144 odel of GPS and demonstrate that the lack of alpha-granules is due to their loss from platelets/matur

145 l levels and were appropriately localized to alpha-granule-like structures.

146 red PDI secretion and impaired exocytosis of alpha granules, lysosomes, and T granules due to decreas

147 rder characterized by deficiency of platelet alpha-granules, macrothrombocytopenia and marrow fibrosi

148 number, as well as maximal secretion of the alpha-granule marker P-selectin; however, their capacity

149 s), and with S12 antibody (to P-selectin, an alpha-granule membrane protein expressed on the platelet

150 The platelet alpha-granule membrane protein P-selectin is expressed a

151 Because alpha-granule molecules are stored in separate compartme

152 , we investigated the pattern of circulating alpha-granule molecules during liver regeneration in 157

153 Secretion of alpha granules (monitored by flow cytometric measurement

154 al platelet counts, platelet morphology, and alpha-granule number, as well as maximal secretion of th

155 Deficiency of COMMD3/CCDC22 causes reduced alpha-granule numbers and overall levels of alpha-granul

156 olipase A2 (sPLA2) is an enzyme found in the alpha granules of platelets and at inflammatory sites.

157 ke growth factor-1 (IGF-1) is present in the alpha granules of platelets and its receptor is expresse

158 Megakaryocyte-derived VWF is stored in alpha-granules of platelets and is enriched in hyperacti

159 id not affect the sorting of P-selectin into alpha-granules of platelets but severely compromised the

160 This indispensable cofactor is stored in alpha-granules of platelets via a poorly understood endo

161 FVIII in megakaryocytes, with storage in the alpha-granules of platelets, has the advantage of delive

162 e most abundant protein contained within the alpha-granules of platelets, is a broad-spectrum inhibit

163 In this study, we show that ATX is stored in alpha-granules of resting human platelets and released u

164 the effects of factors derived from platelet alpha-granules on liver regeneration are unclear, becaus

165 studies, whereas STX8 was not essential for alpha-granule or lysosome secretion, Stx8(-/-) platelets

166 T (dense core granules), platelet factor IV (alpha granules), or hexosaminidase (lysosomes) between n

167 onal agonists and antagonists into different alpha-granule populations.

168 V is highly concentrated within the platelet alpha-granule, previous analysis of human bone marrow an

169 he structure, function, and formation of the alpha-granule protein "coat." Confocal microscopy reveal

170 Platelet factor 4 (PF4), a platelet alpha-granule protein and a soluble cofactor for TM-depe

171 phosphatidylserine (PS) surface expression, alpha-granule protein retention, and lack of active inte

172 latelet factor 4 (PF4), an abundant platelet alpha-granule protein, accelerates in vitro generation o

173 without affecting the release of betaTG, an alpha-granule protein.

174 PN-2 is known to be an abundant platelet alpha-granule protein.

175 reas we observed deficiency of megakaryocyte alpha-granule proteins and emperipolesis.

176 are characterized by high surface levels of alpha-granule proteins and phosphatidylserine, which sup

177 adequate packaging and secretion of selected alpha-granule proteins and reduced secretion of dense gr

178 alpha-Granule proteins bound to COAT-platelets are deriv

179 r-wise comparisons of 15 angiogenic-relevant alpha-granule proteins displayed little, if any, pattern

180 -1 is stabilized by its packaging with other alpha-granule proteins in a calcium-dependent manner.

181 Fractionation of alpha-granule proteins on molecular sieving columns usin

182 ceptionally strong retention of procoagulant alpha-granule proteins on the surface of COAT platelets.

183 The alpha-granule proteins platelet factor 4, beta-thrombogl

184 taining revealed 25-nm diameter complexes of alpha-granule proteins under the electron microscope.

185 approximately 65% to 75% was observed in the alpha-granule proteins von Willebrand factor and P-selec

186 Other alpha-granule proteins were expressed at normal levels a

187 Soluble and membrane-bound alpha-granule proteins were reduced or undetectable, sug

188 alpha-granule numbers and overall levels of alpha-granule proteins, establishing the COMMD3/CCC comp

189 riety of monoclonal antibodies show that the alpha-granule proteins, fibrinogen, von Willebrand facto

190 high levels of phosphatidylserine and retain alpha-granule proteins, including fibrinogen, on their s

191 rred to as 'COAT-platelets', bind additional alpha-granule proteins, including fibrinogen, von Willeb

192 ediated conjugation of serotonin to released alpha-granule proteins.

193 mined whether IFITM3 promoted endocytosis of alpha-granule proteins.

194 uent plasmin-mediated degradation of diverse alpha-granule proteins; and platelet-dependent, accelera

195 Moreover, thrombin-induced platelet alpha-granule release as well as release of adenosine tr

196 dy to syntaxin 4 also inhibited Ca2+-induced alpha-granule release by approximately 75% in this syste

197 Selective modulation of platelet alpha-granule release in patients may represent an attra

198 lets showed enhanced integrin activation and alpha-granule release in response to stimulation of (hem

199 sembles with activation and demonstrate that alpha-granule release is dependent on vesicle SNAP recep

200 Patients with an unfavorable postoperative alpha-granule release profile (high TSP-1/low VEGF) show

201 The unfavorable postoperative alpha-granule release profile was associated with increa

202 isoform, the Pro(33) variant showed greater alpha-granule release, clot retraction, and adhesion to

203 Es and that actin polymerization facilitates alpha-granule release.

204 ne protein completely inhibited Ca2+-induced alpha-granule release.

205 agen-evoked platelet integrin activation and alpha-granule release.

206 The major platelet organelles, alpha-granules, release proteins that participate in thr

207 Although alpha granules released their contents through both mode

208 en recent controversy as to whether platelet alpha-granules represent a single granule population or

209 acrothrombocytopenia and absence of platelet alpha-granules resulting in typical gray platelets on pe

210 nd type 1 plasminogen activator inhibitor in alpha-granule-rich fractions.

211 We show that alpha granule secretion elicited by low agonist doses is

212 rt a mechanism whereby the reported enhanced alpha granule secretion in the Pro(33)-positive platelet

213 surface expression of P-selectin (reflecting alpha granule secretion); (ii) exposure of the fibrinoge

214 global, because other activation responses, alpha granule secretion, shape change, and aggregation w

215 enosine 5'-diphosphate (ADP) restored normal alpha granule secretion, suggesting that the impairment

216 accumulation and affirmed a reduced area of alpha granule secretion.

217 ly inhibits thrombin peptide (SFLLR)-induced alpha-granule secretion (IC(50) = 20 microM) during the

218 TGF-beta1 release is dissociated from alpha-granule secretion (P-selectin up-regulation) and o

219 ma2, leading to downstream responses such as alpha-granule secretion and activation of integrin alpha

220 show that PtdIns(4,5)P(2) mediates platelet alpha-granule secretion and that PtdIns(4,5)P(2) synthes

221 8220 completely inhibited platelet dense and alpha-granule secretion at a concentration of 20 microM

222 GPVI and PAR-induced aggregation, dense and alpha-granule secretion at low agonist concentrations.

223 s of latrunculin A inhibited agonist-induced alpha-granule secretion but stimulated dense granule sec

224 Inhibition of alpha-granule secretion by antihuman cellubrevin was rev

225 ellubrevin antibody inhibited Ca(++)-induced alpha-granule secretion by approximately 50%.

226 inhibited both PtdIns(4,5)P(2) synthesis and alpha-granule secretion by approximately 50%.

227 PtdIns(4,5)P(2) synthesis and Ca(2+)-induced alpha-granule secretion by approximately 50%.

228 SFLLRN-stimulated alpha-granule secretion from SL-O-permeabilized platelet

229 osphate 4-kinase augmented SFLLRN-stimulated alpha-granule secretion from SL-O-permeabilized platelet

230 membrane protein and inhibited Ca2+-induced alpha-granule secretion from streptolysin O-permeabilize

231 Inhibition of actin polymerization prevented alpha-granule secretion in this system, and purified pla

232 ctin cytoskeleton by latrunculin A inhibited alpha-granule secretion induced by several different pla

233 5)P(2) synthesis required for Ca(2+)-induced alpha-granule secretion involves the type II phosphatidy

234 the possibility that morphologic features of alpha-granule secretion may be influenced by the subcell

235 These antibodies inhibited alpha-granule secretion only when added prior to ATP exp

236 Our results confirm that alpha-granule secretion plays a significant role in plat

237 vides a basis for several characteristics of alpha-granule secretion that include homotypic alpha-gra

238 SFLLRN-stimulated alpha-granule secretion was inhibited by a protein kinas

239 Phorbol 12-myristate 13-acetate-stimulated alpha-granule secretion was inhibited by anti-type II Pt

240 Latrunculin A-dependent alpha-granule secretion was inhibited by antibodies dire

241 creases in [Ca(++)](i), Rap1 activation, and alpha-granule secretion were unaffected.

242 leration and augmentation of agonist-induced alpha-granule secretion with comparatively modest effect

243 ed integrin alpha(IIb)beta(3) activation and alpha-granule secretion, (ii) synergistic enhancement of

244 tion of the matrix itself regulates platelet alpha-granule secretion, a key component of platelet act

245 asured by integrin alphaIIbbeta3 activation, alpha-granule secretion, and procoagulant activity.

246 sure of platelet factor XI is independent of alpha-granule secretion, because ADP and collagen expose

247 In addition to stimulating alpha-granule secretion, both SFLLRN and PMA enhanced th

248 bserved that platelet DREAM is important for alpha-granule secretion, Ca(2+) mobilization, and aggreg

249 platelets fail to spread and have decreased alpha-granule secretion, integrin alphaIIbbeta3 activati

250 ulated through GPVI, resulting in defects in alpha-granule secretion, integrin alphaIIbbeta3 activati

251 ying platelet aggregation, dense granule and alpha-granule secretion, P-selectin expression, micropar

252 dIns 3-kinase did not inhibit Ca(2+)-induced alpha-granule secretion, suggesting that PtdIns 3-kinase

253 rmine whether these proteins are involved in alpha-granule secretion, we developed a streptolysin O-p

254 Exogenously added PtdIns(4,5)P(2) inhibited alpha-granule secretion, with 80% inhibition at 50 micro

255 d substitute for platelet cytosol to support alpha-granule secretion.

256 ry system, platelet cytosol was required for alpha-granule secretion.

257 guanosine triphosphate (GTP)-gamma-S-induced alpha-granule secretion.

258 let function, causing stable aggregation and alpha-granule secretion.

259 osphate 4-kinase in agonist-induced platelet alpha-granule secretion.

260 strated that SNARE proteins are required for alpha-granule secretion.

261 reorganization, adhesion to fibrinogen, and alpha-granule secretion.

262 ting that PtdIns 3-kinase is not involved in alpha-granule secretion.

263 time-dependent inhibition of Ca(2+)-induced alpha-granule secretion.

264 ositol 4,5-bisphosphate (PtdIns(4,5)P(2)) in alpha-granule secretion.

265 membrane protein, SNAP-23, and syntaxin 4 in alpha-granule secretion.

266 reptolysin O-permeabilized platelet model of alpha-granule secretion.

267 gregation, despite GPIIb/IIIa activation and alpha-granule secretion.

268 ntiation of myofibroblasts in the absence of alpha-granule secretion.

269 te SEPT5 levels in the maintenance of normal alpha-granule size and may explain the variant granules

270 y bleeding symptoms, the absence of platelet alpha-granules, splenomegaly, and bone marrow (BM) fibro

271 Electron microscopy revealed normal alpha-granule structure in SG-/- platelets.

272 nules and determine whether or not different alpha-granule subpopulations exist in platelets.

273 These studies identify a new alpha-granule subtype expressing VAMP-7 that moves to th

274 Individuals whose platelets lack dense or alpha-granules suffer various degrees of abnormal bleedi

275 pecifically accumulated fibronectin in their alpha-granules, suggesting that fibronectin could be the

276 s more evenly distributed among membranes of alpha-granules, the open canalicular system, and plasma

277 openia with large platelets and a paucity of alpha-granules; these abnormalities cause mostly moderat

278 ation of HYAL2 from a distinct population of alpha-granules to platelet surfaces where it exerts its

279 ts actively sort a specific subpopulation of alpha-granules to the periphery during spreading.

280 zed platelet abnormalities (reduced platelet alpha-granules, unusual stimulated alpha granule content

281 se insulin-like growth factor-1 (IGF-1) from alpha granules upon activation.

282 onectin/PAI-1 complexes were not detected in alpha-granules using a two-site immunoassay.

283 wed similar ultra-structure characteristics (alpha granules, vacuoles and dense tubular system) as PL

284 Addition of calcium ions to intact alpha-granules was an effective means of neutralizing th

285 y of PAI-1 in both platelets and in isolated alpha-granules was prolonged in comparison to the rapid

286 ibodies derived from a rabbit immunized with alpha-granules, was subcloned into the prokaryotic expre

287 Furthermore, platelet alpha-granules were decreased, while vacuoles were incre

288 Proteins that are normally stored in alpha-granules were underrepresented in MVB II and propl

289 count and P-selectin (a ubiquitous cargo of alpha-granules) were not associated with postoperative l

290 other major platelet granules, lysosomes and alpha granules, were apparent.

291 on microscopy revealed a complete absence of alpha-granules, whereas delta-granules were observed.

292 h isoforms are expressed in resting platelet alpha-granules, which are up-regulated to the cell surfa

293 Co-incubation of alpha-granules with a number of agents that disrupt pH g

294 ombospondin-1 (TSP-1) purified from platelet alpha-granules with an apparent K(D) of approximately 7.

295 duced by a GP Ibbeta(Null) genotype revealed alpha-granules with increased size as compared with the

296 ity of PAI-1 activity, whereas incubation of alpha-granules with the calcium ionophore A23187 reduced

297 typic alpha-granule fusion and the fusion of alpha-granules with the open canalicular system and plas

298 Megakaryocytes store BDNF in alpha-granules, with more than 80% of them also containi

299 bleeding disorder characterized by a lack of alpha-granules within blood platelets and progressive bo

300 as associated with uptake of fibrinogen into alpha-granules without surface binding despite pretreatm