ライフサイエンスコーパス: alpha-ketoglutarate

1 nd Kreb's cycle intermediates (succinate and alpha-ketoglutarate).

2 cumulation of 2-hydroxyglutarate and reduced alpha-ketoglutarate.

3 ehydrogenases, efficiently oxidized D-2HG to alpha-ketoglutarate.

4 reductive carboxylation of glutamine-derived alpha-ketoglutarate.

5 glutamate to pyruvate, yielding alanine and alpha-ketoglutarate.

6 potency compared with that of KDM5B at 1 mm alpha-ketoglutarate.

7 rs, JIB-04 is not a competitive inhibitor of alpha-ketoglutarate.

8 nce on the concentration of its co-substrate alpha-ketoglutarate.

9 y of producing 2-hydroxyglutarate (2HG) from alpha-ketoglutarate.

10 levels of O(2)-regulated metabolites such as alpha-ketoglutarate.

11 her converted to 3-deoxy-2-keto-hexarate and alpha-ketoglutarate.

12 tor vs 4PE and a noncompetitive inhibitor vs alpha-ketoglutarate.

13 increase in GDH affinity for its substrate, alpha-ketoglutarate.

14 ination of saccharopine to give l-lysine and alpha-ketoglutarate.

15 r is sequentially converted to glutamate and alpha-ketoglutarate.

16 hile Arg428 contributes mainly to binding of alpha-ketoglutarate.

17 altering stimulation by glucose, leucine, or alpha-ketoglutarate.

18 type PII, and this effect was antagonized by alpha-ketoglutarate.

19 supplementation with TCA cycle intermediate alpha-ketoglutarate.

20 atios of succinate to glutamate, fumarate to alpha-ketoglutarate, 2HG to glutamate, and D-2HG to L-2H

21 xpression due to the increased production of alpha-ketoglutarate, a critical substrate for prolyl hyd

22 glutamate export and that supplementation of alpha-ketoglutarate, a key downstream metabolite of glut

23 omote transcription and, thus, production of alpha-ketoglutarate, a key metabolite in the regulation

24 Loss of Nrd1 or Ogdh leads to an increase in alpha-ketoglutarate, a substrate for OGDH, which in turn

25 low and NADPH production, an accumulation of alpha-ketoglutarate, aconitate, and citrate that is asso

26 ell pairs to demonstrate that treatment with alpha-ketoglutarate (aKG) esters elicits rapid death of

27 alpha-Ketoglutarate (AKG) is a key intermediate of trica

28 astic cells leads to increased production of alpha-ketoglutarate (aKG) within mesenchymal stem cells

29 was present, the antagonism between ADP and alpha-ketoglutarate allowed each of these effectors to i

30 However, HPV was not increased by 1 mm alpha-ketoglutarate alone, and HPV in the absence of alp

31 , IDH1 and IDH2, decarboxylate isocitrate to alpha-ketoglutarate (alpha-KG) and reduce NADP to NADPH.

32 The mechanism is steady state ordered with alpha-ketoglutarate (alpha-Kg) binding prior to acetyl-C

33 (d-2-HG) is an oncometabolite generated from alpha-ketoglutarate (alpha-KG) by mutant isocitrate dehy

34 ate synthase (HOAS), the E1 component of the alpha-ketoglutarate (alpha-KG) dehydrogenase complex (KD

35 Mononuclear nonheme Fe(II) (MNH) and alpha-ketoglutarate (alpha-KG) dependent halogenases act

36 atory genes necessary for the utilization of alpha-ketoglutarate (alpha-KG) in Pseudomonas aeruginosa

37 rption was also activated by induction of an alpha-ketoglutarate (alpha-KG) paracrine signaling syste

38 ricarboxylic acid cycle metabolism with high alpha-ketoglutarate (alpha-KG) production.

39 ino group from branched-chain amino acids to alpha-ketoglutarate (alpha-KG) thereby regenerating glut

40 ide phosphate (NADPH)-dependent reduction of alpha-ketoglutarate (alpha-KG) to 2-HG.

41 branched-chain amino acids while converting alpha-ketoglutarate (alpha-KG) to glutamate.

42 Glutaminolysis converts Gln into alpha-ketoglutarate (alpha-KG), a critical intermediate

43 Here we show that alpha-ketoglutarate (alpha-KG), a tricarboxylic acid cyc

44 Fe(II)- and alpha-ketoglutarate (alpha-KG)-dependent dioxygenases ar

45 Ten-eleven-translocation (TET) proteins are alpha-ketoglutarate (alpha-KG)-dependent dioxygenases th

46 yclization is catalyzed by the non-heme iron alpha-ketoglutarate (alpha-KG)-dependent SnoK in the bio

47 n the levels of the Krebs cycle intermediate alpha-ketoglutarate (alpha-KG).

48 e dehydrogenases (IDH) convert isocitrate to alpha-ketoglutarate (alpha-KG).

49 uction of D-2-hydroxyglutarate (D-2-HG) from alpha-ketoglutarate (alpha-KG).

50 that produces 2-hydroxyglutarate (2-HG) from alpha-ketoglutarate (alpha-KG).

51 ranslocation (TET) family enzymes, which are alpha-ketoglutarate (alpha-KG)/Fe(II)-dependent dioxygen

52 ity or mitochondrial Ca(2+) uptake increased alpha-ketoglutarate (alphaKG) abundance and the NAD(+)/N

53 ative decarboxylation of isocitrate (ICT) to alpha-ketoglutarate (alphaKG) and the NADPH/CO(2)-depend

54 thways and glutaminolysis yielding increased alpha-ketoglutarate (alphaKG) bioavailability.

55 at the tricarboxylic acid cycle intermediate alpha-ketoglutarate (alphaKG) can both sustain naive mou

56 -dependent conversion of isocitrate (ICT) to alpha-ketoglutarate (alphaKG) in the cytosol and peroxis

57 To investigate the role of alpha-ketoglutarate (alphaKG) in the epimetabolic contro

58 hether a physiologic plasma concentration of alpha-ketoglutarate (alphaKG) influences the kinetic int

59 Alpha-ketoglutarate (alphaKG) is an essential intermedia

60 ukoencephalopathy and a urinary excretion of alpha-ketoglutarate (alphaKG) that was markedly increase

61 enzymatic activity allowing them to convert alpha-ketoglutarate (alphaKG) to 2-hydroxyglutarate (2HG

62 Aliphatic halogenases activate O(2), cleave alpha-ketoglutarate (alphaKG) to CO(2) and succinate, an

63 family of enzymes that use Fe(2+), O(2) and alpha-ketoglutarate (alphaKG) to perform a variety of ha

64 me activity that catalyzes the conversion of alpha-ketoglutarate (alphaKG) to the oncometabolite D-(2

65 ase load can reverse the direction of apical alpha-ketoglutarate (alphaKG) transport in the proximal

66 Glutamine is catabolyzed to alpha-ketoglutarate (alphaKG), a tricarboxylic acid (TCA

67 y, belongs to a recently discovered class of alpha-ketoglutarate (alphaKG), non-heme Fe(II)-dependent

68 anaerobic conditions containing iron(II) and alpha-ketoglutarate (alphaKG), to dioxygen initiates oxi

69 to catalyze the conversion of isocitrate to alpha-ketoglutarate (alphaKG), whereas conferring a gain

70 activates anaplerotic flux from glutamine to alpha-ketoglutarate (alphaKG), which subsequently enters

71 rial the citric acid(TCA) cycle intermediate alpha-ketoglutarate (alphaKG), which via its OXGR1 recep

72 ted mouse models to ask if inhibition of the alpha-ketoglutarate (alphaKG)-dependent dioxygenase Egln

73 FIH is a non-heme Fe(II), alpha-ketoglutarate (alphaKG)-dependent dioxygenase that

74 al (2-His-1-carboxylate) facial triad in the alpha-ketoglutarate (alphaKG)-dependent dioxygenases cla

75 xylases (P4Hs) are mononuclear non-heme iron alpha-ketoglutarate (alphaKG)-dependent dioxygenases tha

76 factor (HIF) prolyl hydroxylases (PHDs) are alpha-ketoglutarate (alphaKG)-dependent dioxygenases tha

77 In particular, the family of alpha-ketoglutarate (alphaKG)-dependent dioxygenases, wh

78 ibiting hypoxia-inducible factor (FIH) is an alpha-ketoglutarate (alphaKG)-dependent enzyme which cat

79 ently discovered class of nonheme Fe(II) and alpha-ketoglutarate (alphaKG)-dependent halogenases, cat

80 For the alpha-ketoglutarate (alphaKG)-dependent nonheme iron enz

81 itions (Th1) were regulated by glutamine and alpha-ketoglutarate (alphaKG)-induced events, in part th

82 sm to maintain a high level of intracellular alpha-ketoglutarate (alphaKG).

83 e 1 (IDH1) reversibly converts isocitrate to alpha-ketoglutarate (alphaKG).

84 pids through reductive carboxylation (RC) of alpha-ketoglutarate (alphaKG).

85 oxidative decarboxylation of isocitrate into alpha-ketoglutarate (alphaKG).

86 models of PDAC leads to the accumulation of alpha-ketoglutarate (alphaKG, also known as 2-oxoglutara

87 tricarboxylic acid cycle (TCA) intermediate, alpha-ketoglutarate, also blocks the transcriptional act

88 socitrate pathway, which generates cytosolic alpha-ketoglutarate, also known as 2-oxoglutarate (2OG).

89 The use of alpha-ketoglutarate (alternatively termed 2-oxoglutarate

90 This is a cell-permeable prodrug form of alpha-ketoglutarate, an important intermediate in the tr

91 and IDH2 and reduces levels of intracellular alpha-ketoglutarate, an obligatory cofactor for various

92 ntributes to mtDNA loss by acting as a toxic alpha-ketoglutarate analog.

93 Signaling of alpha-ketoglutarate and adenylylate energy charge by the

94 toglutarate alone, and HPV in the absence of alpha-ketoglutarate and cysteine was not attenuated by a

95 djustments would ensure the replenishment of alpha-ketoglutarate and glutamate, which provide the car

96 depleted in all TCA cycle substrates between alpha-ketoglutarate and malate despite high rates of glu

97 ecrease in cell proliferation was rescued by alpha-ketoglutarate and overexpression of IDH2, whereas

98 e alpha-keto analog of asparagine), yielding alpha-ketoglutarate and oxaloacetate, respectively.

99 the ternary complex of HygX with cosubstrate alpha-ketoglutarate and putative product hygromycin B id

100 ling the intracellular levels of its product alpha-ketoglutarate and subsequent metabolite fumarate.

101 Citrate, alpha-ketoglutarate and succinate are TCA cycle intermed

102 n to citric acid cycle intermediates such as alpha-ketoglutarate and succinate, NaDC3 transports othe

103 Both alpha-ketoglutarate and the UDP-linked sugar bind in the

104 ycle activity to IDH2-mediated production of alpha-ketoglutarate and through it, the activity of key

105 to IDH1 R132H competitively with respect to alpha-ketoglutarate and uncompetitively with respect to

106 tate flows to succinate both through citrate/alpha-ketoglutarate and via malate/fumarate.

107 s the substrate required addition of Fe(2+), alpha-ketoglutarate, and ascorbic acid, confirming that

108 ndent mitochondrial enzymes, mainly lactate, alpha-ketoglutarate, and branched chain keto-acids.

109 ponent (E3) is associated with the pyruvate, alpha-ketoglutarate, and glycine dehydrogenase complexes

110 EI(Ntr) activity was not affected by alpha-ketoglutarate, and no binding between the EIGAF an

111 (H), the enzyme in a complex with NAD(H) and alpha-ketoglutarate, and the enzyme in a complex with NA

112 x redox enzymes are involved, including four alpha-ketoglutarate- and iron(II)-dependent dioxygenases

113 e levels, but causes a drop in the levels of alpha-ketoglutarate, another output of the pathway and a

114 ells (BMDACs) with dimethyloxalylglycine, an alpha-ketoglutarate antagonist that induces hypoxia-indu

115 nt in its physiological concentration range, alpha-ketoglutarate apparently played a role in only the

116 e: levels of TCA-cycle metabolites including alpha-ketoglutarate are high, and levels of the key regu

117 This enzyme has been shown to use alpha-ketoglutarate as an oxidant to regenerate the oxid

118 atalyze phenazine reduction with pyruvate or alpha-ketoglutarate as electron donors.

119 glutamate production from either pyruvate or alpha-ketoglutarate as potential translatable metabolic

120 d by prolyl 4-hydroxylases that use O(2) and alpha-ketoglutarate as substrates to hydroxylate conserv

121 e specific activity of DapL using ll-DAP and alpha-ketoglutarate as substrates was 24.3 + or - 2.0 nm

122 r was used for GC-MS/MS analysis of alanine, alpha-ketoglutarate, asparagine, aspartic acid, cystathi

123 alytic JmjC domain with conserved Fe(II) and alpha-ketoglutarate binding sites, and displays H3K9me1/

124 ly reconstitutes the cofactor (metal ion and alpha-ketoglutarate) binding characteristics of other st

125 as rescued with exogenous membrane-permeable alpha-ketoglutarate, but not pyruvate or oxaloacetate, s

126 The product, l-lyxonate, is catabolized to alpha-ketoglutarate by a previously characterized pathwa

127 In addition, reductive carboxylation of alpha-ketoglutarate by isocitrate dehydrogenase 1 (IDH1)

128 ild-type IDH1, only hyperpolarized [1-(13)C] alpha-ketoglutarate can be detected.

129 this report we demonstrate that derivatized alpha-ketoglutarate can be used as a strategy for mainta

130 We also show that derivatized alpha-ketoglutarate can permeate multiple layers of cell

131 this proof-of-concept study, that [1-(13)C] alpha-ketoglutarate can serve as a metabolic imaging age

132 reductive carboxylation of glutamine-derived alpha-ketoglutarate (catalyzed by reverse flux through i

133 entify several cellular conditions where the alpha-ketoglutarate/citrate ratio is changed due to an a

134 turbations that result in an increase in the alpha-ketoglutarate/citrate ratio.

135 lglycine (DMOG, 200 mug/g), an antagonist of alpha-ketoglutarate cofactor and inhibitor for HIF PHD,

136 of KIC/glutamine (10/2 mM) did not influence alpha-ketoglutarate concentrations but caused 120 and 33

137 hen ATP was the sole adenylylate nucleotide, alpha-ketoglutarate controlled the extent of PII activat

138 tarate, and no binding between the EIGAF and alpha-ketoglutarate could be detected.

139 (via the gamma-aminobutyric acid pathway or alpha-ketoglutarate decarboxylase/succinic semialdehyde

140 Here we show that the alpha-ketoglutarate dehydrogenase (alpha-KGDH) complex i

141 and organization of the multienzyme complex alpha-ketoglutarate dehydrogenase (alpha-KGDH).

142 , we found an increase in phosphorylation of alpha-ketoglutarate dehydrogenase (alphaKGDH) in female

143 ed chain amino acid dehydrogenase (BCDH) and alpha-ketoglutarate dehydrogenase (KDH).

144 lexes, such as pyruvate dehydrogenase (PDH), alpha-ketoglutarate dehydrogenase (KGDH), and the glycin

145 ial chaperones and assists in the folding of alpha-ketoglutarate dehydrogenase (OGDH), a rate-limitin

146 acid cofactor of pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase and other mitochondria

147 ributions of regulation of the activities of alpha-ketoglutarate dehydrogenase and the aspartate-glut

148 ltiple proteins, including the E2 subunit of alpha-ketoglutarate dehydrogenase and the glutathione S-

149 complexes, including pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase and the glycine cleava

150 The activity of the alpha-ketoglutarate dehydrogenase complex (KGDHC), an ar

151 oxymethyl transferase, and components of the alpha-ketoglutarate dehydrogenase complex in conjunction

152 d its target DLST-the E2 subcomponent of the alpha-ketoglutarate dehydrogenase complex, a rate-contro

153 ced CoA to the reduction of NAD(+) using the alpha-ketoglutarate dehydrogenase complex.

154 oteins, we demonstrate that the pyruvate and alpha-ketoglutarate dehydrogenase complexes directly cat

155 osttranslational lipoylation of pyruvate and alpha-ketoglutarate dehydrogenase complexes, resulting i

156 component of the pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase complexes.

157 complexes, including pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, and branched-chain ke

158 ctivity of a rate-limiting TCA cycle enzyme, alpha-ketoglutarate dehydrogenase.

159 CA cycle enzymes, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase.

160 methylation was changed in 1797 genes [e.g., alpha-ketoglutarate dependent dioxygenase (FTO), interle

161 ibitor treatment promoted GBM survival in an alpha-ketoglutarate-dependent (alphaKG-dependent) manner

162 Two functional subtypes of nonheme Fe(II)/alpha-ketoglutarate-dependent aspartyl beta-hydroxylases

163 n of R172K mutant IDH2 resulted in increased alpha-ketoglutarate-dependent consumption of NADPH compa

164 The KDM5/JARID1 family of Fe(II)- and alpha-ketoglutarate-dependent demethylases remove methyl

165 nded DNA for alkylation damage repair by the alpha-ketoglutarate-dependent dioxygenase AlkBH3.

166 ies its lipid-A by hydroxylation by the Fe2+/alpha-ketoglutarate-dependent dioxygenase enzyme (LpxO).

167 Moreover, we applied a key biosynthetic alpha-ketoglutarate-dependent dioxygenase enzyme in a bi

168 O(6)-demethylation are members of the Fe(II)/alpha-ketoglutarate-dependent dioxygenase family.

169 Inactivation of Fe(II)/alpha-ketoglutarate-dependent dioxygenase gene fr9P led

170 lation didomain protein, DdaD, and an Fe(II)/alpha-ketoglutarate-dependent dioxygenase homologue, Dda

171 iates for the archetypal non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase TauD.

172 metal ions or substrate taurine to TauD, an alpha-ketoglutarate-dependent dioxygenase, alters its UV

173 O(6)-demethylation of thebaine by an Fe(II)/alpha-ketoglutarate-dependent dioxygenase.

174 Iron-(II)/alpha-ketoglutarate-dependent dioxygenases can oxidize 5

175 The AlkB family of Fe(II)- and alpha-ketoglutarate-dependent dioxygenases is a class of

176 Fumarate has been shown to inhibit alpha-ketoglutarate-dependent dioxygenases that are invo

177 mO3 and PtmO6 are two functionally redundant alpha-ketoglutarate-dependent dioxygenases that generate

178 The PHDs are alpha-ketoglutarate-dependent dioxygenases that have low

179 even-translocation) proteins are Fe(ii)- and alpha-ketoglutarate-dependent dioxygenases that modify t

180 o serine synthesis, which in turn stimulates alpha-ketoglutarate-dependent dioxygenases that remove t

181 to epigenetic effects through inhibition of alpha-ketoglutarate-dependent dioxygenases that require

182 droxyglutarate is a competitive inhibitor of alpha-ketoglutarate-dependent dioxygenases' by Xu and co

183 the active-site plasticity of these Fe(II) /alpha-ketoglutarate-dependent dioxygenases, and suggest

184 et-dependent methyltransferases, Fe(II)- and alpha-ketoglutarate-dependent dioxygenases, base excisio

185 e TET proteins, a family of AlkB-like Fe(II)/alpha-ketoglutarate-dependent dioxygenases, can oxidize

186 es inhibit a superfamily of enzymes known as alpha-ketoglutarate-dependent dioxygenases, leading to e

187 unit and establish the role of an iron- and alpha-ketoglutarate-dependent enzyme (Fe/alphaKG) in the

188 stically distinct bifunctional non-heme iron alpha-ketoglutarate-dependent enzyme responsible for the

189 diated by Cur halogenase, a non-haem Fe(ii), alpha-ketoglutarate-dependent enzyme.

190 A third subtype of nonheme Fe(II)/alpha-ketoglutarate-dependent enzymes (IbetaH(His)) hydr

191 e function and genomic localization of these alpha-ketoglutarate-dependent enzymes in the maintenance

192 alternatively activated macrophages require alpha-ketoglutarate-dependent epigenetic reprogramming t

193 DdaC catalyzes Fe(II)- and alpha-ketoglutarate-dependent epoxidation of the covalen

194 We show that Fe(II)- and alpha-ketoglutarate-dependent fat mass and obesity-assoc

195 The alpha-ketoglutarate-dependent hydroxylases and halogenas

196 iggered in vivo by hyperammonemia through an alpha-ketoglutarate-dependent inhibition of the mammalia

197 Aspergillus fumigatus is the first reported alpha-ketoglutarate-dependent mononuclear non-haem iron

198 Notably, we characterized an alpha-ketoglutarate-dependent non-heme Fe(II) dioxygenas

199 Specifically, alpha-ketoglutarate-dependent non-heme iron enzymes, Cit

200 ine (5caC) in three consecutive, Fe(II)- and alpha-ketoglutarate-dependent oxidation reactions.

201 C and 5caC in three consecutive, Fe(II)- and alpha-ketoglutarate-dependent oxidation reactions.

202 ups from nucleic acids by a unique iron- and alpha-ketoglutarate-dependent oxidation strategy.

203 e residue, the inclusion of a non-heme iron, alpha-ketoglutarate-dependent oxygenase for hydroxylatio

204 dentified a conserved group of nonheme iron, alpha-ketoglutarate-dependent oxygenases likely responsi

205 family of histone demethylases are Fe2+- and alpha-ketoglutarate-dependent oxygenases that are essent

206 The enzymatic function of many of these alpha-ketoglutarate-dependent proteins is required for p

207 Residue F159 in taurine alpha-ketoglutarate dioxygenase (TauD) is demonstrated t

208 Alkb homolog 7 (ALKBH7) is a mitochondrial alpha-ketoglutarate dioxygenase required for DNA alkylat

209 ely, blocking serine synthesis or repressing alpha-ketoglutarate-driven demethylation facilitates mal

210 al reductive metabolism of glutamine-derived alpha-ketoglutarate even in normoxic conditions.

211 The Jumonji C domain Fe(II) alpha-ketoglutarate family of proteins performs the majo

212 -D via a pathway initiated by the Fe(II) and alpha-ketoglutarate (Fe/alphaKG)-dependent aryloxyalkano

213 PHF2 belongs to a class of alpha-ketoglutarate-Fe(2)(+)-dependent dioxygenases.

214 The sequential activities of PhnY, an alpha-ketoglutarate/Fe(II)-dependent dioxygenase, and Ph

215 uted by 'ancient' CoA-dependent pyruvate and alpha-ketoglutarate ferredoxin oxidoreductases.

216 reductive carboxylation of glutamine-derived alpha-ketoglutarate for de novo lipogenesis.

217 tulate that steric constraints could prevent alpha-ketoglutarate from undergoing an "off-line"-to-"in

218 AMPKalpha2 regulates alpha-ketoglutarate generation, hypoxia-inducible factor

219 Uniquely, after alpha-ketoglutarate has bound to the mononuclear iron ce

220 Reducing O-GlcNAcylation increases alpha-ketoglutarate, HIF-1 hydroxylation, and interactio

221 dependent carboxylation of glutamine-derived alpha-ketoglutarate in hypoxia is associated with a conc

222 to convert glutamine-derived glutamate into alpha-ketoglutarate in the mitochondria to fuel the tric

223 erated tricarboxylic acid cycle intermediate alpha-ketoglutarate, in turn, serves as the cofactor for

224 by an increase in reductive carboxylation of alpha-ketoglutarate (increased concentrations of 2-hydro

225 ion of the tricarboxyclic acid cycle product alpha-ketoglutarate, indicating the critical function of

226 utamine deficiency, through the reduction of alpha-ketoglutarate, inhibits the AlkB homolog (ALKBH) e

227 Four minireviews deal with aspects of the alpha-ketoglutarate/iron-dependent dioxygenases in this

228 alpha-Ketoglutarate is an important metabolic intermedia

229 Glutamine-derived alpha-ketoglutarate is reductively carboxylated by the N

230 e metabolic fate of hyperpolarized [1-(13)C] alpha-ketoglutarate is studied in isogenic glioblastoma

231 actions with the alpha-carboxylate moiety of alpha-ketoglutarate, is also uniquely positioned to best

232 NMN up-regulated alpha-ketoglutarate (KG) levels in Ndufs4-KO muscle, a m

233 inate (suc(2-)) through glutarate (glu(2-)), alpha-ketoglutarate (kglu(2-)), adipate (adi(2-)), pimel

234 nscription factor RTG1 Furthermore, elevated alpha-ketoglutarate levels also suppress 2HG-mediated re

235 ubunit of the IDH3 heterotetramer, decreased alpha-ketoglutarate levels and increased the stability a

236 Genetic modulation of alpha-ketoglutarate levels demonstrates a key regulatory

237 In contrast, alpha-ketoglutarate levels increase at midlevel heteropl

238 However, enhanced glutamine flux increases alpha-ketoglutarate levels, which in turn increases prol

239 n of IDHs, which correlated with the reduced alpha-ketoglutarate levels.

240 from a virtual screen of RsbU revealed that alpha-ketoglutarate, malate and oxaloacetate bound to th

241 termediates with good sensitivity, including alpha-ketoglutarate, malate, fumarate, succinate, 2-hydr

242 other tricarboxylic acid cycle metabolites (alpha-ketoglutarate, malic acid, and glutamate) in froze

243 of a novel pathogenicity island involved in alpha-ketoglutarate metabolism under anaerobic condition

244 of p53 increases the levels of glutamate and alpha-ketoglutarate, mitochondrial respiration rate, and

245 tivity, carried by Dld3, to convert D-2HG to alpha-ketoglutarate, namely an FAD-dependent transhydrog

246 orbic acid, confirming that KdoO is a Fe(2+)/alpha-ketoglutarate/O(2)-dependent dioxygenase.

247 Although two enzymes that catalyze Fe(2+)/alpha-ketoglutarate/O(2)-dependent hydroxylation of deox

248 upying three coordination sites, a bidentate alpha-ketoglutarate occupying two sites, and an aquo lig

249 strate phosphoenolpyruvate and the inhibitor alpha-ketoglutarate, on the structure and dynamics of EI

250 on with an NAD(+) precursor or its substrate alpha-ketoglutarate or treatment with a poly(ADP ribose)

251 lexes it forms with either the co-substrate (alpha-ketoglutarate) or the substrate (fumitremorgin B).

252 hosphate (SerC and PdxA), we have found that alpha-ketoglutarate, oxaloacetic acid, and pyruvate are

253 ons included an unexpected pathway bypassing alpha-ketoglutarate-oxidizing steps in the tricarboxylic

254 One such enzyme is the 2-oxoglutarate (alpha-ketoglutarate) oxidoreductase (OOR), which catalyz

255 c block could be relieved by addition of the alpha-ketoglutarate precursor glutamate.

256 Accordingly, serine starvation or enforced alpha-ketoglutarate production antagonizes squamous cell

257 aling by fueling serine biosynthesis-derived alpha-ketoglutarate production in breast-cancer-derived

258 xygen atoms of N-oxalylglycine (an analog of alpha-ketoglutarate) provide four coordinations in the e

259 Idh, the enzyme that converts isocitrate to alpha-ketoglutarate, providing mechanistic explanation f

260 Glucose, galacturonic acid, alpha-ketoglutarate, pyruvate, acetoin and acetaldehyde

261 ous type I IFN controls the cellular citrate/alpha-ketoglutarate ratio and inhibits expression and ac

262 we have characterized the stereochemistry of alpha-ketoglutarate reduction by showing that d-2-HGA, b

263 2-oxoglutarate (2-OG or alpha-ketoglutarate) relates mitochondrial metabolism to

264 Mechanistically, addition of exogenous alpha-ketoglutarate replenishes TCA intermediates and re

265 mination of KIC and glutamate to leucine and alpha-ketoglutarate, respectively.

266 he mitochondria via glutamate synthesis from alpha-ketoglutarate resulting in cataplerosis.

267 A cell-permeable ester of alpha-ketoglutarate reversed the low TCA cycle intermedi

268 ession was dependent on the concentration of alpha-ketoglutarate substrate in glioma cell lines and c

269 tricarboxylic acid (TCA) cycle intermediate, alpha-ketoglutarate, suggesting that exogenous glutamine

270 death, which is suppressed by glutamate and alpha-ketoglutarate supplementation.

271 to the tricarboxylic acid cycle intermediate alpha-ketoglutarate through glutaminase and alanine amin

272 s is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate" by Ward and c

273 s is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate".

274 morphic enzymatic activity: the reduction of alpha-ketoglutarate to d-2-hydroxyglutaric acid, which i

275 ine biosynthesis pathway, in addition reduce alpha-ketoglutarate to D-2HG using NADH and represent ma

276 exception of E78A, which exhibits binding of alpha-ketoglutarate to E and E.NADH.

277 SRC-2 stimulated reductive carboxylation of alpha-ketoglutarate to generate citrate via retrograde T

278 ted leucine catabolism and transamination of alpha-ketoglutarate to glutamate, with impaired TCA anap

279 zes reversible transamination of leucine and alpha-ketoglutarate to KIC and glutamate, the first step

280 utaminolysis, the conversion of glutamine to alpha-ketoglutarate to maintain the TCA cycle (anapleros

281 n of a combination of 1 mm cysteine and 1 mm alpha-ketoglutarate to promote sulphide synthesis via th

282 CRCs convert substantially more glutamine to alpha-ketoglutarate to replenish the tricarboxylic acid

283 ase (OOR), which catalyzes the conversion of alpha-ketoglutarate to succinyl coenzyme A (succinyl-CoA

284 that human cells use reductive metabolism of alpha-ketoglutarate to synthesize AcCoA for lipid synthe

285 ctural alteration that leads to catalysis of alpha-ketoglutarate to the oncometabolite D-2-hydroxyglu

286 nfer gain-of-function activity by converting alpha-ketoglutarate to the oncometabolite R-2-hydroxyglu

287 Last, addition of the TCA cycle intermediate alpha-ketoglutarate to the Rb TKO MEFs reversed the inhi

288 duced iron and 2-oxoglutarate (also known as alpha-ketoglutarate) to function, although their affinit

289 Apparently, alpha-ketoglutarate was generated from unlabeled glutama

290 stimulation, net synthesis of glutamate from alpha-ketoglutarate was impaired in GDH-deficient islets

291 The apparent K(m)s of MJ1391 for ll-DAP and alpha-ketoglutarate were 82.8 + or - 10 microM and 0.42

292 ed [1-(13)C]glutamate produced from [1-(13)C]alpha-ketoglutarate were significantly higher in temozol

293 s of glutaminolysis catabolites particularly alpha-ketoglutarate, which are generated in an mTORC2-de

294 sm by increasing production of glutamate and alpha-ketoglutarate, which in turn results in enhanced m

295 hydrogenase expression and the production of alpha-ketoglutarate, which negatively regulate hypoxia-i

296 ing the metabolism of glutamine/glutamate to alpha-ketoglutarate, which, in turn, is metabolized to p

297 n of the enzyme (conversion of isocitrate to alpha-ketoglutarate) while conferring a new enzymatic fu

298 ementation with the mitochondrial metabolite alpha-ketoglutarate, whose synthesis is regulated by RIP

299 (2)) is a Stetter-like conjugate addition of alpha-ketoglutarate with isochorismate.

300 whereby Q-derived glutamate is converted to alpha-ketoglutarate with the concomitant conversion of o