ライフサイエンスコーパス: amino acid residue

1 residue was replaced with a homologous beta-amino acid residue.

2 or to peptides containing a cyclobutane beta-amino acid residue.

3 t, molecular unique assignment to individual amino acid residues.

4 ng and tolerates all of the 20 proteinogenic amino acid residues.

5 mutagenesis was used to exchange individual amino acid residues.

6 ganizer (ROR) to discover disease-associated amino acid residues.

7 Proteins are composed of alpha-amino acid residues.

8 nvolved in degrading oligopeptides with 4-12 amino acid residues.

9 compatible with the arrangement of catalytic amino acid residues.

10 icted to be damaging, and occur at conserved amino acid residues.

11 ate the topological importance of individual amino acid residues.

12 sine residues using structural properties of amino acid residues.

13 y limited to a peptide resolution of 5 to 20 amino acid residues.

14 ences of the two isozymes only differ at six amino acid residues.

15 hemical property and occurrence frequency of amino acid residues.

16 l and yet accounts for 90 of the toxin's 387 amino acid residues.

17 opterins, is stabilized by conserved anionic amino acid residues.

18 sites links receptor activation to specific amino acid residues.

19 to codons encoding evolutionarily conserved amino acid residues.

20 i.e., peptides 1 and 2), each composed of 10 amino acid residues.

21 heme oxidations must occur through mediating amino acid residues.

22 sequons containing and/or flanked by acidic amino acid residues.

23 further by assessing the role of individual amino acid residues.

24 twork of water molecules, chloride ions, and amino acid residues.

25 slational modifications (PTM) of Trp and Arg amino acid residues.

26 cluster without participation of protons or amino acid residues.

27 D(2)O increase the proton affinities of key amino acid residues.

28 O-methyloxymorphone) by targeting additional amino acid residues.

29 e random coil chemical shifts of the natural amino-acid residues.

30 r Trp and is tolerant to other, redox-active amino-acid residues.

31 umber of truncated PEX5 molecules comprising amino acid residues 1-197 than full-length PEX5 molecule

32 ragments or an N-terminal gelsolin fragment (amino acid residues 1-70) in the presence of TRAIL impai

33 urons, we developed a pff species labeled at amino acid residue 114 with the environmentally insensit

34 e interaction region in BB0238 spans only 11 amino acids, residues 120-130.

35 ydrolase domain in the alpha-helical region (amino acid residues 126 to 173).

36 in a GTP-dependent manner and that the Robo1 amino acid residues 1394-1398 are required for this inte

37 es in the phosphodiester band (1081 cm(-1)), amino acid residue (~1396 cm(-1)), and C=O stretching ca

38 namely, Cdc42-T17N (via a single mutation of amino acid residue 17 from the N terminus from Thr to As

39 d specific epitope recognition for ZIKV NS1 (amino acid residues 17-34), which could be a potential s

40 amined a putative cell entry region of TcdB (amino acid residues 1753-1851) for short sequences that

41 Structural modeling shows that this amino acid residue 187 is located within the four helix

42 Deletion of amino acid residues 2-354 (Delta2-354 hERG) or 1-136 of

43 inase K (PK), an N-terminal truncation up to amino acid residue 209 altered the conformation of the S

44 at the interaction region in BB0323 requires amino acid residues 22-200, suggesting that the binding

45 tly, the leucine-to-arginine substitution at amino acid residue 232 (L232R) of PTPN3, a frequent muta

46 Amino acid residues 28 to 39 of the N-terminal membrane-

47 Five highly represented signature amino acid residues (37A, 95K, 224N, and 242N in the M1

48 s to baloxavir can be affected by changes at amino acid residue 38 in the polymerase acidic (PA) prot

49 Deletion of 11 amino acids (residues 50 to 60 or residues 110 to 120) p

50 Four HBx mutants with mutations in amino acid residues 55-60 and 121-126 had a lower degree

51 HBx amino acid residues 55-60 and 121-126 may play an import

52 ularly a glutamic-acid-to-lysine mutation at amino acid residue 627 (E627K) in the 627 domain of the

53 tion method, mutations at seven different M1 amino acids (residue 73, 94, 135, 136, or 138 or a doubl

54 ackbone change of a terminally blocked delta-amino acid residue, a dipeptide mimic, by replacing its

55 d mitochondrial proteins on serine/threonine amino acid residues, a process termed protein O-GlcNAcyl

56 contain a large number of positively charged amino acid residues, a striking example being conotoxins

57 Glycine and small amino acid residues allow close-packing of helices on th

58 physical chemical properties of clusters of amino acid residues along the IDP primary sequence, indi

59 attention has been given to the mutation of amino acid residues along the proton translocating D-cha

60 We found that amino acid residues alpha4Gly-41, alpha4Lys-64, and alph

61 these diverse myomixer orthologs reveals key amino acid residues and a minimal fusogenic peptide moti

62 nvolves protein electrostatics between basic amino acid residues and acidic lipids such as phosphoino

63 -directed mutagenesis to substitute specific amino acid residues and computational analyses to elucid

64 n evolutionary relative of RAG), we identify amino acid residues and domains the acquisition or loss

65 val leaves the positions of all coordinating amino acid residues and most nearby water molecules larg

66 ment of alpha-helix propensities for d-alpha-amino acid residues and propensities of residues with no

67 s, and thus electrical charges, of all other amino acid residues and revealed intricate hydrogen-bond

68 m was characterized and named ToPI1, with 33 amino acid residues and three disulfide bonds.

69 gular prism-with the largest containing >700 amino-acid residues and measuring 11 nm in diameter.

70 stances of methylation can occur on a single amino acid residue, and the relative mass of the modific

71 in the pedigree, affected a highly conserved amino acid residue, and was predicted to be deleterious

72 helix propensities among proteinogenic alpha-amino acid residues, and quantitative comparison with pr

73 y activity, we propose that those C-terminal amino acid residues are a potentially targetable motif o

74 sults show that interactions between charged amino acid residues are important both to directly stabi

75 Several amino acid residues are important for the transport acti

76 It is possible to infer which amino acid residues are in contact by analysing covariat

77 When these amino acid residues are individually mutated, the necros

78 We recently confirmed that a small set of amino acid residues are largely responsible for driving

79 g entire genes, and orthologs for which most amino acid residues are polymorphic.

80 he chemical environment of >20% of the total amino acid residues, as revealed by their NMR chemical-s

81 , we examined the importance of the SagS key amino acid residues associated with biofilm formation (L

82 A transporter subfamily all contain an extra amino acid residue at or near a conserved glycine in tra

83 y and virulence, sequence information on the amino acid residue at P8 might be helpful to estimate th

84 ngs strongly support the hypothesis that the amino acid residue at position 163 of canine cellular pr

85 Here, we focused on the amino acid residue at position 8 (P8) of the proteolytic

86 ghly conserved among all strains, except the amino acid residue at position 8 (P8) that shows a certa

87 leavage of pepsin was most influenced by the amino acid residue at position P1.

88 A binding is a cluster of positively charged amino acid residues between the mRNA entry site and uS3

89 2 and basigin is stabilized through multiple amino acid residues, but Gly-171 and Leu-175 of P2 were

90 study of (*)OH oxidative modification of 13 amino acid residues by using (18)O isotopic labeling.

91 ariance (SCV) tolerance and set-points on an amino acid residue-by-residue basis in NPC1 to different

92 rP derivative) with a single homologous beta-amino acid residue can generate biased agonists that ind

93 wn that divergence of a very small number of amino acid residues can account for the cold temperature

94 Changes in particular gp41 HR1 amino acid residues can apparently affect the relative s

95 tential aggregation prone regions (APRs) use amino acid residue composition and/or sequence pattern f

96 ed seven fungal AA9 LPMOs that differ in the amino-acid residues constituting their subsites.

97 e the effects of mutating Lys-300 of NhaA to amino acid residues containing side chains of different

98 fied two stretches of functionally important amino acid residues containing two conserved histidines.

99 These results delineate the role of amino acid residues contributing to the alpha4:alpha4 su

100 us with a mutated PIMMS2 allele reveals that amino acid residues corresponding to the putative subtil

101 e designed a systematic approach to identify amino acid residues crucial for the expression and stabi

102 domains and characterized by three conserved amino acid residues (Cys-1865, His-1955, and Asp-1975).

103 To identify which specific amino acid residues determine high or low susceptibility

104 erpretation of a causative AGE at a specific amino acid residue difficult.

105 ical analysis of data from 11 proteins (6391 amino acid residues) digested with immobilized porcine p

106 ns with oxygen atoms from two sources, other amino acid residues (E321/E324), and membrane lipids, de

107 onstructed eight internal deletions of 40-60 amino acid residues each, spanning the amino-terminal ha

108 es HA and BMP2, which contained highly basic amino acid residues either at the N-terminus (BMP2) or C

109 nstrated that the phylogenetically conserved amino acid residue, F439, was critical for both transpor

110 An analysis of the amino-acid residues flanking the hydrolyzed bonds reveal

111 l for SOD2 interactions, we substituted each amino acid residue for alanine or more conservative resi

112 lity of the nearby cysteine 67, an essential amino acid residue for the formation of HLA-B27 homodime

113 ues forming intersubunit salt bridges and 21 amino acid residues forming hydrogen bonds between the t

114 FXIII-A2 crystal structure, we identified 12 amino acid residues forming intersubunit salt bridges an

115 erogeneous glycans may be bound to different amino acid residues, forming multiple types of protein g

116 hore used and how it interacts with specific amino acid residues found within the opsin protein seque

117 Oct1 cleaved eight amino acid residues from the N-terminal region of Prx1 i

118 e number of exopeptidases that cleave single amino acid residues from the N-terminus of peptide subst

119 magglutinin (HA) head domain involving three amino acid residues, glycine (G), serine (S), and glutam

120 lete L- to D-epimerization of its C-terminal amino acid residue (>=100:1) and hydrolytic product rele

121 bryos, but the functional role of individual amino acid residues has been unclear because of technica

122 This loop, comprising 11-amino-acid residues, has been shown to be crucial for su

123 Enteritidis (SEn) by a single amino-acid residue, immunization with STmOmpD confers mi

124 Mutagenesis further revealed a conserved amino acid residue implicated in reprotonation.

125 ge is a point mutation in which another H2AX amino acid residue important in the DDR, tyrosine 142 (Y

126 In order to identify additional amino acid residues important for acetyltransferase acti

127 genesis and biochemical analysis to identify amino acid residues important for ACOT activity.

128 modeling and mutational analysis identified amino acid residues important for the preference of ChCA

129 Here, we show that a single amino acid residue in CD28 drove T cell exhaustion and h

130 ene and the presence of a negatively charged amino acid residue in position 163 was readily identifie

131 a variety of aromatic side chains at the aza-amino acid residue in the 4-position.

132 alysis is observed upon changing the achiral amino acid residue in the catalyst (at the i+2 position)

133 through a mechanism dependent on a specific amino acid residue in the HCV NS5A protein.

134 intramolecular proton shuttle, and a charged amino acid residue in the Ig1 domain of the chaperone.

135 interaction is dependent on the penultimate amino acid residue in the W sequence, a conserved, phosp

136 entified mutation affects a highly conserved amino acid residue in the zinc finger domain of ZNHIT3.

137 were interconvertible by switching a single amino acid residue in their active sites.

138 em, we demonstrate that substitution of four amino acid residues in a bacterial lanosterol synthase e

139 d light on this issue, we exchanged selected amino acid residues in a highly conserved stretch within

140 The ability to predict which pairs of amino acid residues in a protein are in contact with eac

141 ers describe the side-chain conformations of amino acid residues in a protein based on the rotational

142 ted mutagenesis on five envelope (E) protein amino acid residues in accordance with the attenuated TM

143 An analysis of amino acid residues in and around the OEC has identified

144 We identify amino acid residues in BaeAB's BaeA subunit important fo

145 nstrated with site-directed mutations of key amino acid residues in C. testosteroni mutants.

146 the effects of photolysis and the binding of amino acid residues in cytochrome c and neuroglobin.

147 Structure-guided alterations of amino acid residues in FePYR1 generated ABA receptor var

148 tivity in vitro and in vivo To determine the amino acid residues in hsp70 that are critical for SOD2

149 ses indicate the importance of specific loop amino acid residues in mediating catalytic activity.

150 Examination of these amino acid residues in more natural accessions revealed

151 Identification of isomeric amino acid residues in peptides and proteins is challeng

152 ino acid cysteine, one of the least abundant amino acid residues in proteins, whose unique chemical a

153 e first time the importance of these unusual amino acid residues in solving long-standing hemolysis i

154 Differences in the amino acid residues in the amino (N)-terminal portion of

155 as an affinity ligand for specific conserved amino acid residues in the antibody.

156 ty profile elucidate the roles of individual amino acid residues in the binding of ATR inhibitors, of

157 x-ray crystallography studies identified key amino acid residues in the binding pocket present in hum

158 with neutralizing maternal IgG that targets amino acid residues in the C-terminal region of the V3 l

159 Results from mutating the critical amino acid residues in the disputed transmembrane (TM) r

160 Moreover, we identify six amino acid residues in the DNA-binding interface of DNMT

161 Five critical amino acid residues in the E2 domain primarily are invol

162 Here we demonstrate that substitutions of 3 amino acid residues in the framework of the TCR variable

163 t Glu(446) and Arg(447) cooperate with other amino acid residues in the GERAMT-binding site for prope

164 Changes in particular amino acid residues in the gp41 HR1 region decreased the

165 The conserved amino acid residues in the loop interacting with CoA wer

166 Five highly represented amino acid residues in the M1 and M2 proteins derived fr

167 th more hydrophobic and strongly interacting amino acid residues in the middle of CDR3alpha and CDR3b

168 nds between the compound and the surrounding amino acid residues in the receptor ligand-binding pocke

169 f PeIA analogs were synthesized by replacing amino acid residues in the second disulfide loop with st

170 s identified, along with the identity of the amino acid residues in the virus-receptor interface.

171 ce variants of CDC42, differing only in nine amino acid residues in their very C-terminal regions, pl

172 a new variant of OsCYP20-2 that truncated 71 amino-acid residues in N-terminal.

173 ng of several proteins also requires the two amino-acid residues in the NS1 N-terminal RNA-binding do

174 expression of different regions of CteG (656 amino acid residues) in uninfected cells revealed that i

175 mar, i.e. the arrangement and frequencies of amino acid residues, in known antimicrobial peptide sequ

176 enzyme soybean lipoxygenase-1 substituted at amino acid residues inferred to impact substrate binding

177 ification, principally the introduction of D-amino acid residues into the elongating peptide.

178 sequences annotated to indicate whether the amino acid residue is located within a hinge-bending reg

179 ent ionization (i.e., on average, every 10th amino acid residue is negatively charged).

180 ability to localize phosphosites to specific amino acid residues is crucial to translating phosphopro

181 oat protein recognition motif or its contact amino acid residues is deleterious for viral assembly.

182 Clostridium difficile TcdB (2366 amino acid residues) is an intracellular bacterial toxin

183 ing site in the chaperone was conserved, the amino acid residue itself varied among different species

184 d CBP acetylate Mastermind-like 1 (Maml1) on amino acid residues K188 and K189 to recruit NACK to the

185 We also identified a crucial amino acid residue K59 in nsp11 since a mutation of it l

186 Mutagenesis analysis showed that the amino acid residue K59 of nsp11 was indispensable for in

187 gion of this molecule was incorrect, and the amino acid residue K79 should not have been depicted in

188 Mutations of these amino acid residues lead to reduced selectivity properti

189 e or by tandem MS of LITPOMS behavior at the amino-acid residue level.

190 the method with mass spectrometry allows for amino-acid-residue-level structural information, a highe

191 vation by VWF D4 requires T7, T8, and the 58-amino acid residue linker between T8 and CUB1.

192 ide chains at the so-called P1 position, the amino acid residue located directly N-terminal to the sc

193 ype 1 (TRPA1) activity and identify critical amino acid residues located in the pore domain.

194 roteins and mutagenesis combined reveals two amino acid residues located in the S5 domain, Ser876 and

195 s and both the chlorin phytol chains and the amino acid residues <6 angstrom from the pigments' porph

196 titive reaction between MGO and nucleophilic amino acid residues (Lys, Arg and Cys) and naringenin in

197 We confirmed that amino acid residues making contact with the ligand in th

198 The E2 amino acid residues mediating the interaction with DCTN6

199 The amino acid residues modulating HDAC1 subcellular localiz

200 y subsets of SSc revealed several associated amino acid residues, mostly in the peptide-binding groov

201 nding interaction is distributed across many amino acid residues; mutation of individual residues did

202 enthalpy of unfolding provides the number of amino acid residues nu participating in the unfolding re

203 ous mutation of complement factor H (cfh) at amino acid residue of 1206 (ie, cfh (W/R) ) alone remain

204 structure-function insight into a conserved amino acid residue of transportins in brain development

205 t of the internal degrees of freedom in each amino acid residue of VVD on its overall dynamics by app

206 ctrostatic charge-based permeability and key amino acid residues of CcmK2 functioning in mediating mo

207 Epigenetic modifications of specific amino acid residues of core histones and their isoforms

208 teins of GLUT5 and GLUT7, here we identified amino acid residues of GLUT5 that define its substrate s

209 We show that RBBP4 binds to the N-terminal amino acid residues of PRDM3 and PRDM16, with a dissocia

210 study the dynamics of phosphorylation of key amino acid residues of synapsin I, which critically impa

211 ransfer the ADP-ribose moiety from NAD(+) to amino acid residues of target proteins, leading to mono-

212 1091kJmol(-1) to withstand the disruption of amino acid residues of the enzyme binding site.

213 , we also studied the importance of critical amino acid residues of the four paralogues on mitochondr

214 esigned to target and engage with particular amino acid residues of the galectin-8N extended carbohyd

215 Amino acid residues of the light-harvesting chlorophyll

216 rboxylate of ODDA-PTX and positively charged amino acid residues of the protein.

217 Here, we demonstrate that the 9 C-terminal amino acid residues of TIMP-1 and the large extracellula

218 s spec-based fragment analysis confirmed key amino acid residues on ROCK that are phosphorylated by S

219 ement of alpha-amino acid residues with beta-amino acid residues on susceptibility to proteolysis and

220 Site-specific grafting of dendrons to amino acid residues on the exterior of the alpha-helical

221 *-) or QA(*-) The identification of specific amino acid residues oxidized by reactive oxygen species

222 n, c.567 G > A, affecting a highly conserved amino acid residue (p.Gly189Arg) of the MRM2 protein.

223 l the van der Waals interactions between key amino acid residues (Phe13, Ile31, Gly32, Gly33, Ser42,

224 Here, we mutated the amino acid residue Phe257, located in the finger domain

225 te specificity is controlled by one specific amino acid residue positioned at the entrance to the cat

226 Six amino acid residues potentially important for cold activ

227 characterization in HEK293 cells to identify amino acid residues potentially relevant for both the ZI

228 Structural adjustments of individual amino acid residues predictably control both the assembl

229 uorescent spectroscopy, we demonstrated that amino acid residues predicted to participate in Fe(II)-b

230 Due to ionizable amino acid residues present on the solvent-exposed surfa

231 s in premature termination of the protein 51 amino acid residues prior to the stop codon, and in conc

232 f alpha-tubulin at conserved lysine 40 (K40) amino acid residue regulates microtubule dynamics and co

233 ite-directed mutagenesis identified the core amino acid residues required for KEAP1-mediated degradat

234 ence of known human proteins sharing the key amino acid residues required for recognition by the TCR,

235 other proteins that requires these same two amino-acid residues required for NS1 RBD dsRNA-binding a

236 We show that the N-terminal 152 amino-acid residue segment of DHX30, denoted DHX30N, pos

237 The TMT overlabeling on the off-target amino acid residues serine, threonine, and tyrosine was

238 Amino acid residues showing above background levels of c

239 ansported peptides were found to contain 3-4 amino acid residues showing strong ACE inhibition.

240 eolytic cleavage site of MuV F, because this amino acid residue shows a striking variability dependin

241 ighly reactive and footprint broadly several amino acid residue side chains on the time scale of subm

242 analytics software (Xenophile) to detect the amino acid residue sites of proteins that were covalentl

243 In step 2, based on conservation of amino acid residues, stability analysis, structural supe

244 n this study, several BnaDGAT1 variants with amino acid residue substitutions in PTMD9 were character

245 rized the mechanisms by which these critical amino acid residue substitutions increased the misfoldin

246 ously revealed that one of the regions where amino acid residue substitutions lead to higher performa

247 gnment and further biochemical analysis, the amino acid residue substitutions that conferred increase

248 When amino acid residue substitutions that increased BnaDGAT1

249 Key amino acid residue substitutions were identified that ma

250 f the BnaDGAT1 variants were affected by the amino acid residue substitutions, and a new kinetic mode

251 Isomeric amino acid residues such as valine (Val) and norvaline (

252 of the pKa values of ion-coordinating acidic amino acid residues suggested that the face-specific pH

253 ailed catalytic mechanism and arrangement of amino acid residues surrounding the chromophore in this

254 This inhibition depends on the identities of amino acid residues surrounding the RF2-GGQ functional m

255 In this study, we identified amino acid residues targeted by potentially protective m

256 Does the M domain methylate an amino acid residue tethered to a thiolation (T) domain o

257 The mutation alters an amino acid residue that is conserved from yeast to human

258 ftware called Xenophile, the identity of the amino acid residue that was adducted can be established,

259 g (beaver) SIRT6 protein and identified five amino acid residues that are fully responsible for their

260 nd to interact strongly with AE1 at specific amino acid residues that are linked to diseases and bloo

261 entity of a CRM, the protein target, and the amino acid residues that are modified to be rapidly esta

262 To identify amino acid residues that are responsible for this invers

263 e delta-subunit that contains several acidic amino acid residues that confer proton-sensitivity and e

264 Our analyses identified five amino acid residues that contribute to RRS1-R(SLH) (1) a

265 lar interface and identified a unique set of amino acid residues that determined its affinity.

266 e non-conservative mutations in several ACE2 amino acid residues that disrupt key polar and charged c

267 xtant enzymes feature precise positioning of amino acid residues that facilitate multiple complex tra

268 otein (VP2) gene are nonsynonymous, altering amino acid residues that fall within, or adjacent to, th

269 We chose 10 amino acid residues that form 5 particularly strong inte

270 reported with variations in the binding-site amino acid residues that interact with AI-2.

271 Most of the amino acid residues that interacted with these compounds

272 nes, we were able to pinpoint three specific amino acid residues that may be driving the change in th

273 ied a combination of natural and non-natural amino-acid residues that was used to generate highly eff

274 egatively charged DNA and positively charged amino acid residues, the translocation speed of DNA can

275 nsive scanning mutagenesis by changing every amino acid residue to Val, Ala, or Gly, and then screeni

276 riterpenoid synthesis and reveals diagnostic amino acid residues to differentiate between sterol and

277 ge equilibria to measure the propensities of amino acid residues to participate in helical secondary

278 atalytic oligomer containing alpha- and beta-amino acid residues to template the ring-closing process

279 on contributions by tryptophan and tyrosine amino acid residues to the antioxidant capacity of SPH f

280 define the fundamental nature of the acidic amino acid residues to the K-PPn target domain.

281 allele knock-in of the most commonly mutated amino acid residue, tyrosine 537, in the estrogen-respon

282 The resulting gamma-amino acid residue was incorporated into a novel gamma/a

283 erived from OVA, in which at least one alpha-amino acid residue was replaced with a homologous beta-a

284 ol, an aryl olefin, an alpha substituent, or amino acid residues was carried out to generate the desi

285 that involves just 7-8 out of a total of 148 amino acid residues was clearly detected.

286 No significant loss of other amino acid residues was observed and none of the most co

287 cently, by precision mapping on all possible amino acid residues, we identified histone serine ADPr m

288 oviding a positive charge at the substituted amino acid residue, were associated with poor survival (

289 urs at all of these sites in each individual amino acid residue, which makes aliphatic peptide sequen

290 Our results illustrate how isomerization of amino acid residues, which may seem to be only a minor s

291 dechain hydrogen bonds, XSuLT annotates each amino acid residue with residue depth, chain and ligand

292 of broadly distributed replacement of alpha-amino acid residues with beta-amino acid residues on sus

293 ene (ESR1) mutations at the tyrosine (Y) 537 amino acid residue within the ligand binding domain on (

294 Here, we uncover an amino acid residue within the NS4A transmembrane domain

295 ense or small in-frame deletions that affect amino acid residues within or adjacent to the protein's

296 del was tested using targeted mutagenesis of amino acid residues within or adjacent to the putative i

297 the structure of apo-hGGT reveal movement of amino acid residues within the active site as the substr

298 ata, and targeted mutagenesis, we identified amino acid residues within the Spike-ACE2 interface, whi

299 investigated the contribution of individual amino acid residues within the TMD of Arabidopsis (Arabi

300 esis study was performed to demonstrate that amino acid residue Y265(7.36) was the unique anchor poin