ライフサイエンスコーパス: aminopurine

1 igated using the fluorescent purine analog 2-aminopurine.

2 using a primer-template complex containing 2-aminopurine.

3 itherto undetected physicochemical form of 2-aminopurine.

4 ted by PKR, and this could be inhibited by 2-aminopurine.

5 e fluorescent adenosine analogue 2'-deoxy, 2-aminopurine.

6 with an emission maximum characteristic of 2-aminopurine.

7 mutator background or after treatment with 2-aminopurine.

8 ctively incorporated the fluorescent probe 2-aminopurine 2'-O-methylriboside (2-AP) into the RRE sequ

9 er of Escherichia coli was investigated by 2-aminopurine (2,AP) fluorescence.

10 Although the use of 2-aminopurine (2-AP) as a probe in stopped-flow analyses o

11 substitutions of the fluorescent A-analog 2-aminopurine (2-AP) at -11 and two other positions in pro

12 By inserting the base analog 2-aminopurine (2-AP) at designated positions in 89 bp and

13 a synthetic DNA primer/template containing 2-aminopurine (2-AP) at the template position opposite the

14 erone activity in the DIS conversion using 2-aminopurine (2-AP) fluorescence and nuclear magnetic res

15 ptophan fluorescence in the polymerase and 2-aminopurine (2-AP) fluorescence in the promoter DNA upon

16 The PKR inhibitor 2-aminopurine (2-AP) inhibited TNF-alpha/IFN-gamma-induced

17 ive incorporation of the fluorescent probe 2-aminopurine (2-AP) into a truncated form of the RRE sequ

18 The fluorescent base analogue 2-aminopurine (2-AP) is commonly used to study specific co

19 Using 2-aminopurine (2-AP) labeled sequences derived from the DI

20 e T7 RNA polymerase was investigated using 2-aminopurine (2-AP) modified promoters.

21 mplate DNA constructs with 1 or 2 adjacent 2-aminopurine (2-AP) nucleotide residues (adenine analogue

22 trates containing the fluorescent reporter 2-aminopurine (2-AP) on the template strand, either at the

23 protein kinase pathway by the addition of 2-aminopurine (2-AP) prior to the ODP arrests CHO cells in

24 specifically labeled with monomer or dimer 2-aminopurine (2-AP) probes to map the local interactions

25 loy short (86 bp) synthetic promoters with 2-aminopurine (2-AP) substitutions in the region that beco

26 ed DNA containing the fluorescent reporter 2-aminopurine (2-AP) to study the reaction pathway of the

27 scription is activated in AMphi and PMphi, 2-aminopurine (2-AP) was used to block dsRNA-mediated acti

28 nalogs [inosine (I), purine riboside (PR), 2-aminopurine (2-AP), 2,6-diaminopurine (2,6-DAP), isoguan

29 2-Aminopurine (2-AP), a fluorescent analog of adenine, has

30 ion of A in the standing start position by 2-aminopurine (2-AP), a fluorescent base analogue.

31 Treatment with 2-aminopurine (2-AP), a serine/threonine kinase inhibitor,

32 2-AP)T5), containing the fluorescent base, 2-aminopurine (2-AP), and dT(pT)15 labeled at its 3'-end w

33 We have used a fluorescent adenine analog, 2-aminopurine (2-Ap), as a probe of local double helical s

34 rporating the fluorescent nucleotide probe 2-aminopurine (2-AP), opposite to the site (AB-APopp) or a

35 The fluorescent adenine base analogue, 2-aminopurine (2-AP), placed opposite an abasic site analo

36 rains are sensitive to the DNA base analog 2-aminopurine (2-AP), we screened for 2-AP-resistant (2-AP

37 Interaction of VP55 with 2-aminopurine (2-AP)-containing primers was associated wit

38 uartz substrate from the DNA base analogue 2-aminopurine (2-AP).

39 to a fluorescent nucleotide reporter group 2-aminopurine (2-AP).

40 se cells with the protein kinase inhibitor 2-aminopurine (2-AP).

41 ituted with the fluorescent reporter base, 2-aminopurine (2-AP).

42 res using the fluorescent adenine analogue 2-aminopurine (2-AP).

43 ) in DNA can be converted to S6-methylthio-2-aminopurine (2-AP-6-SCH3) and 2-aminopurine-6-sulfonic a

44 The fluorescent probe, 2-aminopurine-2'-O-methyl riboside (2-AP) has been selecti

45 The base pair formed between 2-aminopurine (2AP) and cytosine (C) is an intermediate in

46 determined using RNA hairpins labeled with 2-aminopurine (2AP) and monitoring the fluorescence change

47 nucleodyes make good FRET pairs with both 2-aminopurine (2AP) and pyrrolocytosine (PyC).

48 We have used 2-aminopurine (2AP) as a fluorescent probe in the template

49 2-Aminopurine (2AP) fluorescence intensity and decay lifet

50 Circular dichroism spectroscopy and 2-aminopurine (2AP) fluorescence studies show no evidence

51 leotide that is melted by the mtRNAP using 2-aminopurine (2AP) fluorescence that is sensitive to chan

52 been examined using time-resolved FRET and 2-aminopurine (2AP) fluorescence.

53 CAG)n repeat, we have substituted a single 2-aminopurine (2AP) fluorescent base for adenine at select

54 The fluorescent adenine analog 2-aminopurine (2AP) has been used extensively to monitor c

55 2-Aminopurine (2AP) is a fluorescent analog of guanosine a

56 2-Aminopurine (2AP) is an analogue of adenine that has bee

57 erase (T4 pol) to primer-template DNA with 2-aminopurine (2AP) located at the primer terminus results

58 state fluorescence of the adenine analogue 2-aminopurine (2AP) opposite an abasic site demonstrated t

59 In both cases, the base analogue 2-aminopurine (2AP) proved tremendously useful-first as a

60 esses were triggered by photoexcitation of 2-aminopurine (2AP) residues site-specifically positioned

61 have used the fluorescent adenine analogue 2-aminopurine (2Ap) to probe the local double-helical stru

62 Here we describe the application of 2-aminopurine (2AP), a fluorescent isomer of adenine, to r

63 f this riboswitch and the ligands adenine, 2-aminopurine (2AP), and 2,6-diaminopurine (DAP).

64 rchetypical fluorescent nucleoside analog, 2-aminopurine (2Ap), has been used in countless assays, th

65 ssed using the fluorescent purine analogue 2-aminopurine (2AP), incorporating 2AP between purine and

66 rescence lifetimes of the adenine analogue 2-aminopurine (2AP), replacing adenine opposite the uracil

67 employed, containing the fluorescent base, 2-aminopurine (2AP), substituted at the -11 position in a

68 In many of these analogues, such as 2-aminopurine (2AP), the fluorescence is quenched when inc

69 se-modified nucleotides 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, and inosine which would mak

70 methylthio-2-aminopurine (2-AP-6-SCH3) and 2-aminopurine-6-sulfonic acid (2-AP-6-SO3H) upon reaction

71 The fluorescent nucleotide 2-aminopurine (a*) reports mainly on base stacking.

72 ommonly used fluorescent ribonucleoside is 2-aminopurine, a highly responsive purine analogue.

73 Since 2-aminopurine, a serine/threonine kinase inhibitor that ha

74 A 6-aminopurine (adenine) derivative of bis(2,2'-bithienyl)m

75 Substitution of 2-aminopurine adjacent to the target base also results in

76 Finally, replacement of adenine by 2-aminopurine (AG --> 2-APG) had no effect on the second s

77 luorescence measurements of DNA containing 2-aminopurine allowed presteady-state real time observatio

78 thymine is just the first excited state of 2-aminopurine alone.

79 treatment of porcine and bovine cells with 2-aminopurine, an inhibitor of PKR, increased the yield of

80 ing the population of an initially excited 2-aminopurine, an isomer of adenine, we can follow the cha

81 It relies on strategic incorporation of 2-aminopurine, an isosteric fluorescent adenosine analogue

82 ng model with two intermediates, while the 2-aminopurine analogs required one intermediate.

83 ichroism (CD), fluorescence of adenine --> 2-aminopurine analogs, and fluorescence resonance energy t

84 Various 2-aminopurine analogues of AdA were synthesized, all of wh

85 lexes carried fluorescent DNA base analogs 2-aminopurine and 1,3-diaza-2-oxophenoxazine as environmen

86 Studies with the nucleotide analogues 2-aminopurine and 2,6-diaminopurine indicated that this st

87 Prior studies of 2-aminopurine and 7-deazaguanine effects had shown that th

88 e in photostability of a DNA base analogue 2-aminopurine and a coumarin derivative (7-HC) in 10-nm sp

89 sphorylation of PKR and inhibitors of PKR, 2-aminopurine and adenine, ablated poly(I:C)-induced gene

90 The fluorescence emission spectra of the 2-aminopurine and FRET derivatives suggest greater solvent

91 ein kinase R (PKR) dependent (abrogated by 2-aminopurine and greatly reduced in PKR-/- cells).

92 ped a rapid fluorescence-based assay using 2-aminopurine and measured the steady-state rate constants

93 By using 2-aminopurine and purine as the templating residues, which

94 The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is

95 e) of a hydrogen-bonded complex containing 2-aminopurine and thymine is just the first excited state

96 ecies is not a covalently modified form of 2-aminopurine and we suggest that it represents a hitherto

97 oorly in the presence of the base analogue 2-aminopurine, and exposure to the base analogue results i

98 ements with site-specifically incorporated 2-aminopurine, and functional assays indicate that the nat

99 ce of the yield of CT between photoexcited 2-aminopurine (Ap) and G through DNA bridges of varied len

100 -FB) which uses the fluorescent bases (FB) 2-aminopurine (AP) and pyrrolo-dC (P-dC) as fluorophores.

101 lex by examining photoinduced quenching of 2-aminopurine (Ap) as a result of hole transfer (HT) to gu

102 equence-specific hydration dynamics, using 2-aminopurine (Ap) as the intrinsic fluorescence probe and

103 s of analogous primer-templates containing 2-aminopurine (AP) at the primer 3' terminus indicate that

104 nformational changes upon catalysis, while 2-aminopurine (AP) fluorescence assays have detected conco

105 e, we use the fluorescent guanine analogue 2-aminopurine (AP) in nucleotide position 76, immediately

106 2-Aminopurine (AP) is a highly mutagenic base analog.

107 RNA that is based on the use of the CD of 2-aminopurine (AP) residues as a probe.

108 The base analog 2-aminopurine (AP) strongly promotes A.T to G.C and G.C to

109 n which a fluorescent nucleobase analogue, 2-aminopurine (AP), occupies defined positions with respec

110 s-cleaving HDV ribozyme to the fluorescent 2-aminopurine (AP), we can directly monitor local conforma

111 nd DNA:RNA hybrids containing photoexcited 2-aminopurine (Ap).

112 Using 2-aminopurine as a dangling end purine base, we have emplo

113 With 2-aminopurine as a fluorescent reporter in the DNA substra

114 Using 2-aminopurine as a site-specific fluorescent probe in plac

115 s the recognition of 2,6-diaminopurine and 2-aminopurine, as confirmed in crystal structures of respe

116 2-Aminopurine at positions +3, +4, or +5 in the nonscissil

117 ymerase binding to promoters incorporating 2-aminopurine at positions -4 through -1 support a model w

118 to synthetic tRNA(1)(Leu) substituted with 2-aminopurine at positions 36 and 37, fluorescence energy

119 apture the ultrafast decay dynamics of the 2-aminopurine base as the ligand, we have detected the pre

120 ergy transfer from normal DNA bases to the 2-aminopurine base in synthesized DNA oligomers were inves

121 bonucleotide substrate containing a uracil:2-aminopurine base pair.

122 Single 2-aminopurine bases are introduced into otherwise standard

123 On binding CCE1, 2-aminopurine bases located at the point of strand exchang

124 conformational probes comprising pairs of 2-aminopurine bases site-specifically replacing adenine ba

125 Stacking 2-aminopurine between two guanine moieties is shown to sig

126 )M(-1)s(-1) and 2.1 x 10(5)mM(-1)s(-1) for 2-aminopurine binding the adenine-responsive mutant ribosw

127 educed markedly by treatment of cells with 2-aminopurine but not by genistein.

128 cosylase activity on 2-aminopurine/G and A/2-aminopurine but weaker activity on A/C than E. coli MutY

129 Stopped-flow fluorescence studies using 2-aminopurine-containing oligodeoxyribonucleotides further

130 GTP binding to polymerase and fluorescent 2-aminopurine-containing promoter DNA complex.

131 The ability of protein-Ca(2+) to rearrange 2-aminopurine-containing substrates was monitored by low e

132 a a disulfide bond, 2'-deoxy-6-(cystamine)-2-aminopurine (d6Cys2AP) was synthesized and incorporated

133 )-primer-template (P/T) complex containing 2-aminopurine (dAP) and a metal exchange-inert Rh(III) der

134 Equilibrium binding studies utilizing a 2-aminopurine deoxypseudouridine DNA substrate showed that

135 At neutral pH, RNAs with adenine, 2-aminopurine, dimethyladenine or purine substitutions at

136 evealed upon GTP binding to the polymerase.2-aminopurine DNA complex.

137 NA bending by FRET and DNA unpairing using 2-aminopurine exciton pair CD to determine the DNA and pro

138 d filamentation phenotypes associated with 2-aminopurine exposure are effectively suppressed by null

139 the IRE-RNA, altering its conformation (by 2-aminopurine fluorescence and ethidium bromide displaceme

140 By using 2-aminopurine fluorescence as the base flipping probe we f

141 omic force microscopy (AFM) supported by a 2-aminopurine fluorescence base flipping assay to study da

142 shable observed rate constants of FRET and 2-aminopurine fluorescence changes indicate that DNA bendi

143 Similarly, stopped-flow 2-aminopurine fluorescence changes showed that promoter op

144 methionine to the M.EcoKI:DNA complex, the 2-aminopurine fluorescence changes to that of a new specie

145 Stopped-flow fluorometry monitoring the 2-aminopurine fluorescence defined the kinetics of uracil

146 2-Aminopurine fluorescence experiments indicate that this

147 The slowest step detected by 2-aminopurine fluorescence increase is assigned to the fin

148 ion of the correct NTP to the T7 RNAP-DNA, 2-aminopurine fluorescence increased rapidly and exponenti

149 Thus it appears that 2-aminopurine fluorescence intensity is not a clear indica

150 Base-flipping kinetics (monitored using 2-aminopurine fluorescence intensity) were essentially syn

151 The increase in 2-aminopurine fluorescence is specific to the editing site

152 M247 appears to be unimportant, but 2-aminopurine fluorescence measurements show that Y261 pla

153 us DNA duplexes that is based on combining 2-aminopurine fluorescence measurements with a new quantit

154 ransfer analysis showed that a decrease in 2-aminopurine fluorescence occurs only when AdoMet is pres

155 sly reported that ADAR2 induced changes in 2-aminopurine fluorescence of a modified substrate, consis

156 similar, and both NC and Gag affected the 2-aminopurine fluorescence of bases close to the loop bind

157 Here, we use NMR and 2-aminopurine fluorescence spectroscopy to examine how DMS

158 nal dynamics, as detected by time-resolved 2-aminopurine fluorescence spectroscopy.

159 fluorescence resonance energy transfer and 2-aminopurine fluorescence studies reveals that DNA bendin

160 We used 2-aminopurine fluorescence to monitor promoter melting and

161 n uses both steady-state and time-resolved 2-aminopurine fluorescence to show pronounced unwinding of

162 el22 by circular dichroism (CD), intrinsic 2-aminopurine fluorescence, and fluorescence resonance ene

163 Using 2-aminopurine fluorescence-based equilibrium and kinetic m

164 ence resonance energy transfer (FRET), and 2-aminopurine fluorescence.

165 lex formation leads to a rapid increase of 2-aminopurine fluorescence.

166 ces cerevisiae has been investigated using 2-aminopurine fluorescence.

167 er of the DNA junction that is observed by 2-aminopurine fluorescence.

168 ated CT across adenine tracts monitored by 2-aminopurine fluorescence.

169 eported directly from the mismatch site by 2-aminopurine fluorescence.

170 gic and systematic single-substitutions of 2-aminopurine for adenine bases.

171 The substitution of 2-aminopurine for adenine on the probe DNA sequence enable

172 SpMYH has greater glycosylase activity on 2-aminopurine/G and A/2-aminopurine but weaker activity on

173 Mutations G8(inosine), G8(diaminopurine), G8(aminopurine), G8(adenosine), and G8(uridine) folded prop

174 was completely inhibited by chelerythrine, 2-aminopurine, genistein, and W-7 and only partially or no

175 We find that 2-aminopurine gives enhanced fluorescence emission not onl

176 The fluorescence of 2-aminopurine has been previously shown to depend on the e

177 Use of the fluorescent base analogue 2-aminopurine has provided a direct demonstration of the t

178 of magnitude (from 5.9 nM to 0.59 mM) for 2-aminopurine in a 1 cm x 0.17 cm fluorescence cuvette.

179 M.PspGI enhances fluorescence of 2-aminopurine in DNA if it replaces the second C in the se

180 monitoring the increase in fluorescence of 2-aminopurine in DNA-T7 RNA polymerase complexes, we obtai

181 h, we insert the fluorescent base analogue 2-aminopurine in place of A1492 in an E. coli 16S rRNA A-s

182 tes containing the fluorescent base analog 2-aminopurine in place of adenine at specific positions in

183 NA internally labeled with the base analog 2-aminopurine in place of adenine to monitor transcription

184 ated DNA containing the fluorescent analog 2-aminopurine in place of the target adenine.

185 y CD spectra and fluorescence lifetimes of 2-aminopurine in substrates and products that were indicat

186 folding kinetics of a ribozyme containing 2-aminopurine in the loop of P9.

187 FRET between end labels or fluorescence of 2-aminopurine in the stem as conformational probes, yield

188 Specific inhibition of PKR by 2-aminopurine in these FA BM cells attenuates PKR activati

189 ad high-affinity (K(i) = 0.2-4 microM) for 6-aminopurines, including adenine, 2'-deoxyadenosine, and

190 The fluorescence of 2-aminopurine increases when the DNA goes from a double-st

191 urine metabolism in this genus by steering 6-aminopurines into 6-oxypurines.

192 ame cannot be said for structures in which 2-aminopurine is base stacked with other nucleobases.

193 dimer, and no changes are detected if the 2-aminopurine is moved opposite the 3'-thymine of the pyri

194 tructure is available, the fluorescence of 2-aminopurine is often used as a signal for base flipping

195 the identity of the nucleobase with which 2-aminopurine is stacked.

196 s on the femtosecond dynamics of boxB RNA (2-aminopurine labeled in different positions), through mut

197 gate the nature of PAP domain flexibility, 2-aminopurine labeled molecular probes were employed in st

198 peptides using fluorescent titrations with 2-aminopurine labeled versions of the three GNRA-folded lo

199 udies presented include the utilization of 2-aminopurine-labeled DNA substrates, 2-aminopurine nucleo

200 mission maximum of a duplex substrate with 2-aminopurine located at the editing site, consistent with

201 ctive enzyme, the correct cofactor and the 2-aminopurine located at the methylation site.

202 rements of a fluorescent adenine analogue (2-aminopurine) located at the 3'-primer terminus.

203 Our 2-aminopurine mapping studies show that the LSP (Light Str

204 minoguanine, highlights the specificity of 8-aminopurine-mediated ICL production.

205 A series of 13 new (S,Z)-2-aminopurine methylenecyclopropane analogues was synthesi

206 showed that HX RNA carrying a fluorescent 2-aminopurine modification provides a model system that ca

207 and abasic modifications and G378/379 with 2-aminopurine, N7-deazaguanosine, and 6-thioguanosine.

208 ch with a single, strategically positioned 2-aminopurine nucleobase substitution.

209 diazotization-dediazoniation of two types of aminopurine nucleoside derivatives has been investigated

210 s not only from N(6)-substituted purine or 2-aminopurine nucleoside monophosphates but also from O(6)

211 cally acts at the 6-position of purine and 2-aminopurine nucleoside monophosphates.

212 with a quantum yield comparable to that of 2-aminopurine nucleoside.

213 phosphorolysis of natural 6-oxopurine and 6-aminopurine nucleosides was observed, with adenosine the

214 n of 2-aminopurine-labeled DNA substrates, 2-aminopurine nucleotide triphosphate, a nonhydrolyzable n

215 ing site-specific substitution of 2'-deoxy-2-aminopurine nucleotides at key adenosine positions.

216 nfluence of Gag on the fluorescence of the 2-aminopurine nucleotides at the base of the helix implied

217 pocket in a conformation analogous to the 6-aminopurine of ATP.

218 the fluorescence intensity of one or more 2-aminopurine or 6-methylisoxanthopterin base analogs inco

219 In cells pre-exposed to the PKR inhibitor 2-aminopurine or in PKR-null cells, the activation of p38

220 mplexes, alteration of a guanine to either 2-aminopurine or nebularine resulted in an increase in K(d

221 anges were observed with G:pyrrolo dC or T:2-aminopurine pairs.

222 The 2-aminopurine phosphoramidite prepared by this method coup

223 was not affected by inhibition of PKR with 2-aminopurine, phosphorylation of MKK3/6 and p38 as well a

224 Substitutions with 2-aminopurine probe base exposure throughout this structur

225 y conformational transition, reported by a 2-aminopurine probe, that takes place in the open ternary

226 2-Aminopurine probes at the upstream ends of the hairpin s

227 ht bases upstream of the pause site, while 2-aminopurine probes show that the elongation bubble exten

228 coupling in the CD spectra of dimer 2-AP (2-aminopurine) probes at various positions in the ssDNA co

229 be readily hydrogenated to give purine or 2-aminopurine products in good yield.

230 d by one-electron oxidation of 8-oxoGua by 2-aminopurine radicals generated by the two-photon ionizat

231 The fluorescent nucleobase 2-aminopurine replaced three individual adenines, two of w

232 mechanism for selective hydrolysis of the 2-aminopurine residue in alkaline solution is predominantl

233 The presence and location of the 2-aminopurine residue is easily verified by treatment of t

234 is of oligodeoxyribonucleotides containing 2-aminopurine residues at selected sites.

235 ectra of site-specifically placed pairs of 2-aminopurine residues have been used to probe the roles o

236 We located the 2-aminopurine residues in the presumed melting domain of t

237 observing the increase in fluorescence of 2-aminopurine residues incorporated in the oligos.

238 generated by the two-photon ionization of 2-aminopurine residues site specifically positioned in 5'-

239 gy CD and fluorescence spectra of pairs of 2-aminopurine residues that have been inserted at defined

240 The advantage of using pairs of 2-aminopurine residues, inserted at defined nucleic acid p

241 Pyrrolocytosine or 2-aminopurine residues, site-specifically substituted for

242 This method involves protection of the 2-aminopurine ribonucleoside, reduction to the deoxyribonu

243 tes including 6-oxopurine ribonucleosides, 6-aminopurine ribonucleosides, and to a lesser extent puri

244 e only permissive growth conditions were a 6-aminopurine source in the presence of 2'-deoxycoformycin

245 ntum yield of nucleotide analogues such as 2-aminopurine strongly depends on base stacking interactio

246 Our 2-aminopurine studies show that helicase and polymerase bo

247 e of RNA polymerase and DNA fragments with 2-aminopurine substituted at specific positions.

248 asor diagrams is demonstrated here using a 2-aminopurine substituted telomeric G-quadruplex sequence.

249 semble, and fluorescence measurements with 2-aminopurine-substituted 3A-DNA provided initial tests of

250 The effects of 2-aminopurine substitution on the physical and structural

251 of the HIV-1 packaging signal (Psi), using 2-aminopurine substitution to create a series of modified

252 perties of this hairpin are assessed using 2-aminopurine substitutions for adenine at six positions i

253 se stacking on fluorescence quantum yield, 2-aminopurine substitutions for adenine previously demonst

254 The fluorescence properties of the 2-aminopurine substitutions showed features consistent wit

255 Fluorescence experiments using 2-aminopurine suggest that LNA modifications enhance base

256 mutation frequency, the dam mutants had a 2-aminopurine-susceptible phenotype that could be suppress

257 iation rates; e.g., the protein releases a 2-Aminopurine:T base pair approximately 90-fold faster tha

258 es site specifically positioned in 5'-d(CC[2-aminopurine]TC[8-oxoGua]CTACC).

259 By analyzing changes in fluorescence of a 2-aminopurine template base undergoing replication in real

260 This probe is up to 50-fold brighter than 2-aminopurine, the fluorescent nucleoside standard.

261 ored using the fluorescence intensities of 2-aminopurines, the changes in the intensity relative to t

262 experiment showing that installation of a 2-aminopurine-thymine base pair at the cross-linking site

263 The Trypanosoma brucei aminopurine transporter P2/TbAT1 has long been implicate

264 the genus Trypanosoma brucei through the P2 aminopurine transporter.

265 The fluorescent adenine analogue 2-aminopurine was incorporated at various single positions

266 The fluorescent nucleotide 2-aminopurine was substituted at selected sites within the

267 Using the fluorescent probe 2-aminopurine, we show that inhibitors interact with highe

268 Using single substitutions of adenine with 2-aminopurine, we show that intrastrand folding in repeate

269 thyltransferase (GAATTC) was replaced with 2-aminopurine, which fluoresces upon excitation at 310 nm.

270 Like 2-aminopurine, which substitutes for adenine bases, the fl