ライフサイエンスコーパス: amphiphysin

1 ending in the context of the N-BAR domain of amphiphysin.

2 locked selectively by a truncation mutant of amphiphysin.

3 exists independently of dynamin-1 binding to amphiphysin.

4 ated by the conserved NH2-terminal region of amphiphysin.

5 identified a cDNA that encodes a Drosophila amphiphysin.

6 inding to the Src homology 3 (SH3) domain of amphiphysin.

7 via a pathway dependent on both dynamin and amphiphysin.

8 phiphysin/Rvs (BAR) proteins, endophilin and amphiphysin.

9 -tubule formation requires the BAR domain of amphiphysin.

10 this role may differ from that of vertebrate amphiphysins.

11 Amphiphysin 1 and 2 are proteins implicated in the recyc

12 accessory factors, among which amphiphysin (amphiphysin 1 and 2) is one of the best characterized.

13 sis for altering the binding of dynamin 1 to amphiphysin 1 and Grb 2 by site-directed mutants mimicki

14 hysin 1 knockout mice and found that lack of amphiphysin 1 causes a parallel dramatic reduction of am

15 Amphiphysin 1 colocalizes with p35 in the growth cones o

16 We have defined here amino acids of amphiphysin 1 crucial for binding to AP-2 and clathrin.

17 Since binding of cain to amphiphysin 1 does not affect amphiphysin's interaction

18 ression in Chinese hamster ovary cells of an amphiphysin 1 fragment that binds both AP-2 and clathrin

19 We show here that amphiphysin 1 interacts with the cdk5-activating subunit

20 Amphiphysin 1 is a phosphoprotein expressed at high leve

21 Amphiphysin 1 is also phosphorylated by the cdc2/cyclin

22 We have now generated amphiphysin 1 knockout mice and found that lack of amphi

23 dynamin 1 with the Src homology 3 domain of amphiphysin 1 was reduced.

24 The interaction of amphiphysin 1 with either clathrin or AP-2 did not preve

25 proteins implicated in endocytosis-dynamin, amphiphysin 1, amphiphysin 2 and synaptojanin.

26 Among these proteins is amphiphysin 1, implicated in clathrin-mediated endocytos

27 regulating the interaction of dynamin 1 with amphiphysin 1.

28 her than reduced the binding of dynamin 1 to amphiphysin 1.

29 a stable association with the SH3 domain of amphiphysin 1.

30 Amphiphysins 1 and 2 are enriched in the mammalian brain

31 The structures of the human amphiphysin-1 SH3 domain complexed with 884 peptides wer

32 We show a proof-of-concept study on the amphiphysin-1 SH3 domain interacting with its peptide li

33 eractions for the binding specificity of the amphiphysin-1 SH3 domain.

34 Here, we focused on amphiphysin 2 (BIN1, also known as bridging integrator-1

35 In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and v

36 cated in endocytosis-dynamin, amphiphysin 1, amphiphysin 2 and synaptojanin.

37 An isoform of amphiphysin 2 concentrated at T-tubules induced tubular

38 Like mammalian amphiphysin 2 in muscles, Drosophila amphiphysin does no

39 in 1 causes a parallel dramatic reduction of amphiphysin 2 selectively in brain.

40 Shorter amphiphysin 2 splice variants are also found ubiquitousl

41 M1), while mutations in dynamin 2 (DNM2) and amphiphysin 2/BIN1 (AMPH2) cause milder forms of myopath

42 ing to the clathrin adaptors AP2, EPS15, and amphiphysin 2/Bin1.

43 preciable frequency was noted for AB against amphiphysin (2.0%), ARHGAP26 (1.3%), CASPR2 (0.9%), MOG

44 we have solved the crystal structure of the amphiphysin-2 (Amph2) SH3 domain to 2.2 A.

45 rotein spanning residues 1728-1744 binds the amphiphysin-2 (BIN1) Src homology-3 (SH3) domain with an

46 have evolved a superior binding affinity for amphiphysin-2 SH3 compared with typical cellular ligands

47 ch as dynamin, thereby enabling hijacking of amphiphysin-2 SH3-regulated host cell processes by these

48 ensive negatively charged binding surface of amphiphysin-2 SH3.

49 orphologically similar to those generated by amphiphysin, a curvature-inducing protein involved in en

50 etch homologous to a corresponding region in amphiphysin, a protein previously shown to have similar

51 or dominant negative dynamin K44A, epsin 2a, amphiphysin A1, and clathrin light chain but enhanced by

52 The mechanism of amphiphysin action in recruiting dynamin was additionall

53 ids, these findings support a model in which amphiphysin acts as a multifunctional adaptor linking th

54 mutant and lack of phenotypes suggests that Amphiphysin acts redundantly with other proteins to orga

55 found that lap modulated the localization of amphiphysin (Amph), the homologue of another AD risk fac

56 a variety of accessory factors, among which amphiphysin (amphiphysin 1 and 2) is one of the best cha

57 ptors, which includes the mammalian proteins amphiphysin and Bin1 and the yeast proteins Rvs167p and

58 ions of dynamin and two associated proteins, amphiphysin and clathrin, were examined in the retinas o

59 The interactions of dynamin with amphiphysin and endophilin are essential for the formati

60 We show that at low concentrations, amphiphysin and endophilin, but not SNX9 or the curvatur

61 ased, type I clathrin-binding sequence, both amphiphysin and epsin contain a second, distinct sequenc

62 ence indicating a direct interaction between amphiphysin and membrane lipids, these findings support

63 microvillus initiation WASp colocalizes with amphiphysin and moesin.

64 ent kinase family is a conserved property of amphiphysin and suggest that this phosphorylation may pl

65 wo other proteins implicated in endocytosis (amphiphysin and synaptojanin) has suggested a potential

66 The membrane-remodeling proteins Amphiphysin and Syndapin colocalize with Past1 in distin

67 ding sequences in the adaptor beta subunits, amphiphysin, and beta-arrestin, facilitates the associat

68 We found dynamin, syndapin, amphiphysin, and calcineurin, a regulator of activity-de

69 d by concomitant depletion of endophilin and amphiphysin, and conversely, depletion of dynamin dramat

70 n other accessory proteins, including AP180, amphiphysin, and epsin.

71 an genome, represented by the Arfaptin, Bin1/Amphiphysin, and IRSp53 BAR domains.

72 egative for onconeural (Hu, Yo, Ri, CV2, Tr, amphiphysin, and Ma2), glutamic acid decarboxylase, and

73 proline-rich region of synaptojanin 1, Grb2, amphiphysin, and members of SH3p4/8/13 protein family, o

74 the retromer-linked sorting nexin (SNX)-Bin, Amphiphysin, and Rvs (BAR) proteins leads to a pronounce

75 AP) containing Src homology 3 (SH3) and Bin, amphiphysin, and RVS161/167 (BAR) domains, is a substrat

76 tis elegans, localizing via its F-BAR (Bin1, Amphiphysin, and RVS167) domain and a flanking 200-amino

77 he endocytic proteins dynamin, clathrin, and amphiphysin are expressed and broadly distributed in IHC

78 The amphiphysins are brain-enriched proteins, implicated in

79 ns heterodimerize, further suggests that the amphiphysins are closely connected with dynamin-mediated

80 3 (SH3) domains of vertebrate and Drosophila amphiphysins are highly similar, supporting the putative

81 sophila nucleotide database using vertebrate amphiphysin as a query identified a cDNA that encodes a

82 Cell wall removal is dependent on Fus2p, an amphiphysin-associated Rho-GEF homolog.

83 role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiolo

84 ts from our simulation of a single adsorbing Amphiphysin BAR dimer indicate that it is capable of sta

85 We solved the structure of the Drosophila amphiphysin BAR domain.

86 The tubules are marked by amphiphysin but are otherwise poorly understood.

87 rminal Bin/amphiphysin/Rvs (N-BAR) domain of amphiphysin, by building models of N-BAR both bound to a

88 al amphipathic helix and BAR domain (N-BAR), amphiphysin can drive membrane curvature in vitro and in

89 as well as the mammalian proteins CIN85 and amphiphysin, carry ubiquitin-binding SH3 domains.

90 rked reduction or loss of progenitor markers Amphiphysin, Cited1, Sall1 and Pax2.

91 ng dynamin was additionally tested in vitro: amphiphysin could associate with both dynamin and alpha-

92 representative N-BAR domains from Drosophila amphiphysin (dAmp-BAR) and rat endophilin A1 (EndoA1-BAR

93 r, several recent reports using a Drosophila amphiphysin (damph) null mutant have failed to substanti

94 sates using mass spectrometry and identified Amphiphysin (dAmph).

95 in distinct SSR subdomains and collapse into Amphiphysin-dependent membrane nodules in the SSR of pas

96 In addition, amphiphysin depletion was observed to severely inhibit c

97 cellular dialysis with peptide P4, a dynamin/amphiphysin-disrupting peptide, increased whole-cell gly

98 mmalian amphiphysin 2 in muscles, Drosophila amphiphysin does not bind clathrin, but can tubulate lip

99 ly affect associations of the appendage with amphiphysin, eps15, epsin, and AP180, revealing a common

100 Amphiphysin family members are implicated in synaptic ve

101 BIN1 is a member of the amphiphysin family of proteins and has reported roles in

102 BIN1 is a member of the amphiphysin family of proteins, and contains N-terminal

103 mour suppression--for certain members of the amphiphysin family.

104 ning deficits, suggesting a critical role of amphiphysin for higher brain functions.

105 tch accounts for the specific requirement of amphiphysin for two arginines in the proline-rich bindin

106 Rvs) domain is the most conserved feature in amphiphysins from yeast to human and is also found in en

107 Flies that lack all Amphiphysin function are viable, lack any observable end

108 wever, there has been no genetic analysis of Amphiphysin function in higher eukaryotes.

109 the three proteins studied, only full-length amphiphysin functions synergistically with full-length D

110 uscle rigidity (glutamic acid decarboxylase, amphiphysin, GABA(A)-receptor-associated protein, or gly

111 , a two thermal cycle LDR was carried out on amphiphysin gene transcripts that can serve as important

112 AMPA-R); GABA regulation and release (GAD65, amphiphysin); glycine receptors (GLY-R); water channels

113 w that BAR-domain scaffolds from endophilin, amphiphysin, GRAF, and beta2-centaurin limit membrane fi

114 -1 > collapsin response-mediator protein-5 > amphiphysin > Purkinje cell cytoplasmic antibody-2 = ANN

115 its endocytic role, Vps1 functions with the amphiphysin heterodimer Rvs161/Rvs167 to facilitate scis

116 heromone-induced protein associated with the amphiphysin homologue Rvs161p, which is required for cel

117 Unlike amphiphysin, however, synucleins and apolipoproteins do

118 Here we report that amphiphysin I (Amph I) is also a Mnb/Dyrk1A substrate.

119 Now we have explored the possibility that amphiphysin I also may have a role in actin dynamics and

120 We show here that amphiphysin I also undergoes constitutive phosphorylatio

121 We also have found that amphiphysin I and dynamin I colocalize in developing neu

122 evidence for a close functional link between amphiphysin I and dynamin I.

123 t calcineurin-dependent dephosphorylation of amphiphysin I and of its two major binding proteins is p

124 Amphiphysin I has the opposite effect.

125 Amphiphysin I is an abundant presynaptic protein that in

126 Amphiphysin I is an SH3 domain-containing neuronal prote

127 electron microscopy immunocytochemistry that amphiphysin I is localized in the nerve terminal cytomat

128 Western blot analysis revealed that amphiphysin I levels steadily increased with neuronal di

129 ation, whereas in antisense-treated cultures amphiphysin I levels were reduced to approximately 10% o

130 Dephosphorylation of amphiphysin I requires extracellular Ca2+ and is unaffec

131 s and cell polarity by testing the effect of amphiphysin I suppression on neurite outgrowth.

132 The NH(2) terminus of amphiphysin I was critical for both inhibition of and bi

133 These data support the hypothesis that amphiphysin I, dynamin I, and synaptojanin I are physiol

134 Rvs167 and Rvs161, the yeast homologs of amphiphysin I, have been implicated in endocytosis, acti

135 Dephosphorylation of amphiphysin I, like dephosphorylation of dynamin I and s

136 have now been purified and identified as the amphiphysins I and II, which forms a heterodimer that al

137 efects were complemented by Bin1, but not by Amphiphysin-I, arguing that these genes have distinct fu

138 ody evaluation in 2015, was 0.024%, equaling amphiphysin IgG (0.026%) and more common than ANNA-2 (al

139 Patients with isolated amphiphysin-IgG (n = 19) were more likely to be women (w

140 Amphiphysin-IgG was identified in 71 patients among 120,

141 llapsin response-mediator protein-5 -IgG and amphiphysin-IgG, predict specific cancers in the setting

142 il treatment involves the BAR domain protein Amphiphysin II (BIN1) and the restoration of catecholami

143 We demonstrate here that a novel isoform of amphiphysin II associates with early phagosomes in macro

144 Thus, amphiphysin II facilitates a critical initial step in ho

145 The mammalian Bin1/Amphiphysin II gene encodes an assortment of alternative

146 recruit amphiphysin II to the phagosome, and amphiphysin II in turn recruits dynamin.

147 s protein and shown that this mutant form of amphiphysin II inhibits phagocytosis at the stage of mem

148 In both these locations, amphiphysin II is colocalized with splice variants of an

149 Amphiphysin II participates in receptor-mediated endocyt

150 cascade in which PI3K is required to recruit amphiphysin II to the phagosome, and amphiphysin II in t

151 , bridging integrator 1 (Bin1, also known as amphiphysin II), and vesicle-associated membrane protein

152 equence (417)PWDLW originally found in human amphiphysin II.

153 species, also termed amphiphysin isoforms or amphiphysin II.

154 ly conserved and ubiquitously expressed Bin1/Amphiphysin-II and Bin3 genes.

155 Abrogation of amphiphysin IIm function results in C. pneumoniae-induce

156 We report here that, although amphiphysin IIm is usually only transiently associated w

157 The data suggest that C. pneumoniae retains amphiphysin IIm on the vacuole to survive within the mac

158 The adaptor protein, amphiphysin IIm, participates in phagocytosis and is tra

159 CA-1), PCA-2; and CRMP-5-immunoglobulin G or amphiphysin-immunoglobulin G.

160 Expressions of either amphiphysin in COS-7 cells reduced activity of endogenou

161 ta are consistent with a role for Drosophila amphiphysin in endocytosis, but the details of this role

162 imultaneously, further supporting a role for amphiphysin in endocytosis.

163 and, more generally, a physiological role of amphiphysin in membrane deformation.

164 ated by DYRK1A phosphorylation in binding to amphiphysin in vitro.

165 ble complex in cells with Bin1, but not with amphiphysin, in a BAR domain-dependent manner.

166 g to amphiphysin, whereas phosphorylation of amphiphysin inhibited its binding to AP-2 and clathrin.

167 Bacterially expressed recombinant amphiphysins inhibited both PLD1 and PLD2 isozymes in vi

168 is the most potent clathrin terminal domain-amphiphysin inhibitor reported to date.

169 e assembly of dynamin 1, synaptojanin 1, and amphiphysin into complexes that also included clathrin a

170 Amphiphysin is a single-copy gene that maps to position

171 Amphiphysin is an SH3 domain-containing neuronal protein

172 Messenger RNA of this amphiphysin is expressed widely during embryogenesis and

173 is study challenges the notion that synaptic Amphiphysin is involved exclusively in endocytosis and s

174 hiphysin were not recruited, indicating that amphiphysin is involved in localizing dynamin to the fus

175 he Drosophila larval neuromuscular junction, amphiphysin is localized postsynaptically and amphiphysi

176 We show that Drosophila Amphiphysin is localized to actin-rich membrane domains

177 We therefore propose that muscle amphiphysin is not involved in clathrin-mediated endocyt

178 We propose that amphiphysin is not only required for synaptic-vesicle en

179 Amphiphysin is phosphorylated by cdk5 in a region includ

180 In vertebrates, Amphiphysin is postulated to function during endocytosis

181 Amphiphysin is present in these cells at a low level and

182 containing proteins, such as endophilins and amphiphysins, is integral to the process of endocytosis.

183 l groups of a second nerve terminal-enriched amphiphysin isoform, and the finding that the two protei

184 in actin function and suggest that distinct amphiphysin isoforms contribute to define distinct domai

185 Amphiphysin isoforms have a putative role in membrane de

186 in brain-specific BIN1 species, also termed amphiphysin isoforms or amphiphysin II.

187 he RVS161 or RVS167 Saccharomyces cerevisiae amphiphysin-like gene confers similar growth phenotypes

188 Saccharomyces cerevisiae cells lacking the amphiphysin-like orthologs, Rvs161 or Rvs167, are unable

189 The cDNA encoding ALP1 (amphiphysin-like protein 1) was isolated from a 16-day m

190 interaction with Fus2p, a pheromone-induced amphiphysin-like protein.

191 Regulation of these processes by calcium and amphiphysin may provide a mechanism for controlling the

192 itro with a potency similar to that of brain amphiphysin (median inhibitory concentration of approxim

193 Similarly to the case with amphiphysin, mouse rod terminals showed stronger clathri

194 Amphiphysin mutants have a novel phenotype, a severely d

195 mphiphysin is localized postsynaptically and amphiphysin mutants have no major defects in neurotransm

196 For the relatively well-studied case of amphiphysin N-BAR domain, one suggested mechanism involv

197 m and coarse-grained simulations of multiple amphiphysin N-BAR domains and their components interacti

198 n, plays a key role in membrane sculpting by amphiphysin N-BAR domains.

199 re emphasized by simulations of oligomerized amphiphysin N-BARs at the atomistic and coarse-grained l

200 The absence of tubules in amphiphysin-null embryos correlates with faster cleavage

201 Misexpression of Amphiphysin outside its normal membrane domain in photor

202 ction between GluA2 and PICK1 or dynamin and amphiphysin prevented the depression of transmission, su

203 Our findings suggest a conserved role of the amphiphysin protein family in the dynamics of the cortic

204 machinery that orchestrates the process.The amphiphysin protein has recently emerged into the limeli

205 Surprisingly, endophilin and amphiphysin recruitment to membranes was also dependent

206 Four other alternately spliced exons encode amphiphysin-related sequences that were included in brai

207 ents suggested that the inhibitory effect of amphiphysins results from their direct interaction with

208 FCH-BIN amphiphysin RVS (F-BAR)/SH3 domain proteins play key rol

209 Here we show that the Fer/Cip4 homology-Bin amphiphysin Rvs protein Hof1, which has known roles in c

210 Membrane remodeling by BAR (Bin, Amphiphysin, RVS) domain-containing proteins, such as en

211 The newly described F-BAR (Fer/CIP4 and Bin, amphiphysin, Rvs) family of proteins includes Cdc42-inte

212 Bin-Amphiphysin-Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR)

213 Bin-Amphiphysin-Rvs (BAR) and Fes-CIP4 homology BAR (F-BAR)

214 Studies on the Bin-Amphiphysin-Rvs (BAR) domain have advanced a fundamental

215 e concave surface of the crescent-shaped Bin-amphiphysin-Rvs (BAR) domain is postulated to bind to th

216 IM) is a member of newly emerged inverse Bin-Amphiphysin-Rvs (BAR) domain protein family and a putati

217 TPase-activating (Rho-GAP) domain, and a Bin-Amphiphysin-Rvs (BAR) domain.

218 The crescent-shaped bin-amphiphysin-rvs (BAR) domains remodel membranes in multi

219 inds endophilin and other members of the Bin-Amphiphysin-Rvs (BAR) protein family.

220 PACSIN3 lacking the Bin-Amphiphysin-Rvs (F-BAR) domain interacted with TRPV4 wit

221 ily of proteins, and contains N-terminal Bin-Amphiphysin-Rvs and C-terminal Src homology 3 domains.

222 Three C. albicans genes that encode Bin-Amphiphysin-Rvs homology domain proteins were mutated to

223 It contains a Bin-Amphiphysin-Rvs membrane-binding domain with an N-termin

224 ntified a novel F-BAR (Fes/CIP4 homology-Bin-Amphiphysin-Rvs protein)-containing RhoGAP, Rgd3, that h

225 rise an N-terminal membrane-curving BAR (Bin-amphiphysin-Rvs) domain linked to a PH domain and a C-te

226 4 homology)/F-BAR (FER-CIP4 homology and Bin-amphiphysin-Rvs) domain of FBP17 was previously shown to

227 ing (G) proteins of the Arf family, BAR (Bin-amphiphysin-Rvs) domain proteins, and protein kinase D.

228 The BAR (Bin-Amphiphysin-Rvs) domain undergoes dimerization to produc

229 ins PICK1, dependent upon the PICK1 BAR (Bin-amphiphysin-Rvs) domain, and that interacts with the Glu

230 whether SH3 domains from the F-BAR (FCH-Bin-Amphiphysin-Rvs) subfamily of membrane-deforming protein

231 iffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Al

232 ial formation through IRSp53, an Inverse-Bin-Amphiphysins-Rvs (I-BAR) domain protein.

233 Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR)

234 sent in the middle of Tuba upstream of a Bin/amphiphysin/Rvs (BAR) domain activates Cdc42, but not Ra

235 a close interaction with endophilin A1's BIN/amphiphysin/Rvs (BAR) domain and deep insertion of its a

236 Proteins of the Bin/Amphiphysin/Rvs (BAR) domain family, in particular, play

237 For the SNX-BAR subgroup, a Bin/Amphiphysin/Rvs (BAR) domain is vital for formation/stab

238 Interacting with C Kinase 1 (PICK1) is a Bin/Amphiphysin/Rvs (BAR) domain protein involved in AMPA re

239 Endophilin is a bin/amphiphysin/rvs (BAR) domain protein that induces vesicl

240 Bin/Amphiphysin/Rvs (BAR) domain proteins control the curvat

241 Proteins containing a Bin/Amphiphysin/Rvs (BAR) domain regulate membrane curvature

242 Endophilin contains a Bin/Amphiphysin/Rvs (BAR) domain responsible for membrane be

243 Proteins of the Bin/amphiphysin/Rvs (BAR) domain superfamily are essential i

244 , which also have a curvature-generating Bin/Amphiphysin/Rvs (BAR) domain, have membrane-remodeling f

245 drolysis to this effect, assisted by the BIN/amphiphysin/Rvs (BAR) domain-containing protein endophil

246 Bin/Amphiphysin/Rvs (BAR) domain-containing proteins are ess

247 (PDZ) domain and a central lipid-binding Bin/amphiphysin/Rvs (BAR) domain.

248 Cellular proteins containing Bin/amphiphysin/Rvs (BAR) domains play a key role in clathri

249 The Bridging integrator 1 (BIN1)/Amphiphysin/Rvs (BAR) protein family is an essential par

250 romer-related Sorting Nexin family (SNX)-Bin/Amphiphysin/Rvs (BAR) proteins can directly recognize ca

251 membrane recruitment of dynamin with the BIN/amphiphysin/Rvs (BAR) proteins, endophilin and amphiphys

252 We show that an N-terminal Bin/Amphiphysin/Rvs (BAR)-like domain in Num1 mediates the a

253 Anillin Mid1p, Fes/CIP4 homology-Bin/amphiphysin/Rvs (F-BAR) Cdc15p, IQ motif containing GTPa

254 FER/Cip4 homology-Bin/amphiphysin/Rvs (F-BAR) domain family proteins form cres

255 Inverse Bin/Amphiphysin/Rvs (I-BAR) domains drive local membrane def

256 Collective action by inverse-Bin/Amphiphysin/Rvs (I-BAR) domains drive micron-scale membr

257 coarse-grained models of the N-terminal Bin/amphiphysin/Rvs (N-BAR) domain of amphiphysin, by buildi

258 MIM) is a defining member of the inverse Bin/Amphiphysin/Rvs domain (I-BAR) subfamily of lipid bindin

259 static interactions between endophilin's BIN/Amphiphysin/Rvs domain and anionic membrane lipids have

260 ent recruitment of N-terminal containing BIN/Amphiphysin/RVS domain containing (N-BAR) proteins.

261 Using a coarse-grained model of Bin/amphiphysin/Rvs domain with an N-terminal helix (N-BAR)

262 cruitment of the intracellular SphK1 and Bin/Amphiphysin/Rvs domain-containing proteins endophilin-A2

263 he hypothesis that, even in mammalian cells, amphiphysin/Rvs family members have a role both in endoc

264 BAR (Bin/Amphiphysin/Rvs) adapter proteins have been suggested to

265 The BAR (Bin/amphiphysin/Rvs) domain is the most conserved feature in

266 mponent Argounaute-2 (Ago2) and the BAR (bin/amphiphysin/rvs) domain protein PICK1.

267 Endophilin N-BAR (N-terminal helix and Bin/amphiphysin/Rvs) domain tubulates and vesiculates lipid

268 R-binding, PDZ (PSD-95/Dlg/ZO1) and BAR (Bin/amphiphysin/Rvs) domain-containing protein PICK1 has bee

269 of membrane curvature generation by BAR (Bin/amphiphysin/Rvs) domains is thought to involve the plast

270 nd also encode curvature-generating BAR (Bin/Amphiphysin/Rvs) domains.

271 Endophilin A1 is a BAR (Bin/amphiphysin/Rvs) protein abundant in neural synapses tha

272 toplasmic side of the MCC, including the Bin/amphiphysin/Rvs-domain proteins Pil1 and Lsp1, which ass

273 endophilin N-terminal amphipathic helix Bin/Amphiphysin/Rvs-homology (N-BAR) domain is unique becaus

274 l mutations in three late endocytic factors: amphiphysins (rvs161 and rvs167) and verprolin (vrp1).

275 es with the endocytic reporters Abp1 and the amphiphysin Rvs167.

276 The yeast homologue of amphiphysin, Rvs167, functions in endocytosis and actin

277 hways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Px

278 Membrane remodeling by Fes/Cip4 homology-Bin/Amphiphysin/Rvs167 (F-BAR) proteins is regulated by auto

279 ing of cain to amphiphysin 1 does not affect amphiphysin's interaction with other endocytic proteins,

280 end of the proline-rich domain, whereas the amphiphysin SH3 binds Site 9 (Pro-833-Pro-836) toward th

281 clathrin cages and with dynamin via the GST-amphiphysin SH3 domain.

282 Our data suggest that amphiphysin SH3 domains are important regulators of the

283 nstrate that the interaction of dynamin with amphiphysin SH3 domains, unlike that with SH3 domains of

284 usly described consensus sequence PXRPXR for amphiphysin SH3 ligands is inaccurate and instead define

285 site overlaps the phosphorylation sites, but amphiphysin-SH3 binding was unaffected.

286 Preloading of dynamin-1 PRD with the amphiphysin-SH3 domain partially occluded binding of the

287 larly, recombinant glutathione S-transferase*amphiphysin-SH3 domain, but not a mutated form that cann

288 interaction appeared to take place with the amphiphysin-SH3 domain; this bound to a single high affi

289 However, the fly amphiphysin shows less conservation to sequences in the

290 Thus, the inhibition of PLD by amphiphysins, synaptojanin, and AP180 might play an impo

291 tic proteins and lipids, including dynamins, amphiphysin, syndapin, endophilin, and PIP2, which are r

292 conservation to sequences in the vertebrate amphiphysins that bind other endocytic components such a

293 In the central segment of amphiphysin, the type I and type II sequences cooperate

294 e results can be explained by the binding of amphiphysin to the NH(2)-terminal domain of clathrin and

295 Labeling for dynamin, clathrin, and amphiphysin was distributed differentially among convent

296 A putative endocytic function of amphiphysin was supported by dominant-negative interfere

297 rom a giant vesicle in a solution containing amphiphysin, we observed that the action of the protein

298 Caveolin-3 and amphiphysin were implicated in their biogenesis.

299 Dynamin mutants unable to bind amphiphysin were not recruited, indicating that amphiphy

300 nd synaptojanin 1 inhibited their binding to amphiphysin, whereas phosphorylation of amphiphysin inhi

301 tified as an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin,