ライフサイエンスコーパス: ampholyte

1 s according to their pI values without using ampholytes.

2 rom ITP experiments with fluorescent carrier ampholytes.

3 l generated by impurities present within the ampholytes.

4 ct the human absolute bioavailability of the ampholytes.

5 tion limits were 310 +/- 30 zmol with pH 4-8 ampholytes.

6 icroclimate pH effects in the case of acidic ampholytes.

7 al generated by impurities within commercial ampholytes.

8 conditions, without the addition of carrier ampholytes.

9 ocusing (cIEF) using a set of amino acids as ampholytes.

10 ) in the detection of nonfluorescing carrier ampholytes.

11 ctric points (pI) without the use of carrier ampholytes.

12 er ITP and displace subsets of these carrier ampholytes.

13 tions including high levels of contaminating ampholytes and complex fractionation and isolation proce

14 f the chamber, we used free solution carrier ampholytes and immobilized acrylamido buffers in the PA

15 label a mixture of low-concentration carrier ampholytes and introduce it into an isotachophoresis (IT

16 unlabeled analytes using fluorescent carrier ampholytes and isotachophoresis (ITP).

17 eins in a straight channel using broad-range ampholytes and then refocusing segments of the first cha

18 sic groups and are distinguished as ordinary ampholytes and zwitterions.

19 d on the charge to mass without resorting to ampholytes and/or isoelectric focusing, using a single-

20 fractions to be collected, washed to remove ampholyte, and analyzed by RPLC-MS.

21 nstrated with little or no interference from ampholyte, and CIEF-RPLC-MS data are used to construct a

22 itive interference with the aminoglycosides, ampholytes, and phenothiazines and negative interference

23 Broad-range carrier ampholytes at about 0.05% were found to be most effectiv

24 e by 2 cm long in 3-10 min using broad-range ampholytes at electric field strengths ranging from 25 t

25 ed-silica capillary in a solution of carrier ampholytes at physiological pH and osmolarity, where the

26 ject effluent from the IEF dimension into an ampholyte-based CE separation.

27 This paper reports the application of ampholyte-based isoelectric focusing in poly(dimethylsil

28 Photobleaching reduced the noise in the ampholyte blank by an order of magnitude.

29 rated using bovine serum albumin in a single ampholyte buffer as well as in multiple-component buffer

30 Using single-component ampholyte buffers with well-defined pI cutoff values, co

31 increases essentially in presence of carrier ampholyte (CA) components, which makes problematic a rel

32 showed that the ITP displacement of carrier ampholytes can be used for detection of unlabeled (nonfl

33 However, IEF assays often use carrier ampholytes (CAs) to establish a pH gradient for protein

34 Ampholytes contain both acidic and basic groups and are

35 assays to interference from aminoglycosides, ampholytes, detergents, phenothiazines, reducing agents,

36 rves relating effective mobility and carrier ampholyte displacement at two different leading electrol

37 rge number (on the order of 1000) of carrier ampholytes enables detection of a wide range of analytes

38 I-MS interface to reduce (desalt) amino acid ampholytes in-line after cIEF and prior to electrospray

39 an overcome these problems but is limited by ampholyte interference and signal quenching in ESI-MS.

40 shown to be separated using a linear carrier ampholyte (linear pH gradient between two electrodes) of

41 lyte spacers added to the sample or by using ampholyte mixtures to form a continuum of spacers.

42 ocusing performed with photobleached pH 3-10 ampholytes produced concentration detection limits of 27

43 The carrier ampholytes provide a large number of fluorescent species

44 The concentration of ampholytes required for cIEF is mutually exclusive with

45 ied by analyzing the gaps in the fluorescent ampholyte signal.

46 and thus the electrophoretic mobility-of the ampholyte sums to zero.

47 the addition of a small pI range (4-6.5) of ampholytes to improve resolution and stability of the co

48 m charge arising from covalent attachment of ampholytes to the benzophenone-functionalized gel matrix

49 With the elimination of ampholytes, we can analyze the fractionated proteins dir

50 e explained by partial neutralization of the ampholytes when droplets become more concentrated in the

51 an accurate measure of the amount of carrier ampholytes which are focused between the leading electro

52 o 10 microL/min and does not require carrier ampholytes, which can create molecular backgrounds for s

53 h the exception of two compounds, all of the ampholytes with bioavailability <50% were predominantly

54 reduce the background signal, we photobleach ampholytes with high-power photodiodes.

55 The isoelectric carrier ampholyte zones could be detected as inverted peaks due