ライフサイエンスコーパス: amplicon

1 icked to mix the detection reagents with the amplicon.

2 a Forkhead box E1 (FOXE1)-containing hybrid amplicon.

3 op probe and the targeted fragment of a ZIKV amplicon.

4 e chromatin topology on the extrachromosomal amplicon.

5 uencing of internal transcribed spacer (ITS) amplicons.

6 at requires nanopore sequencing of small DNA amplicons.

7 ng intermediate amplicons as primers for end amplicons.

8 Amplicon 16 S rRNA sequencing suggested that EPA and DHA

9 ality in cancer cells that contain the 17q23 amplicon, a recurrent copy number aberration that define

10 First, Indel Detection by Amplicon Analysis (IDAA) determines the size and frequen

11 tion, of which 167 primers gave expected PCR amplicon and 101 primers showed polymorphism.

12 Using high-throughput amplicon and metagenome sequencing, combined with X-ray

13 and functional gene diversity (16S rRNA gene amplicon and metagenomic sequencing analyses), as well a

14 Here, we combine advances in amplicon and metagenomic sequencing with culture-enriche

15 ination of cultivation-independent analyses (amplicon and shot-gun sequencing) and cultivation-depend

16 Amplicon and shotgun DNA sequencing data of microbial ma

17 We integrate gut microbiome 16S rRNA amplicon and shotgun metagenomic sequence data with quan

18 rs to perform phylogenetic regression on 16S amplicon and shotgun sequencing data and to visualize re

19 recent advances in applications of targeted amplicon and shotgun-based metagenomics approaches to in

20 thods achieved to date are based on targeted amplicon and unbiased metagenomic shotgun NGS approaches

21 as well as sequencing bacterial and archaeal amplicons and community functional genes.

22 mplification of the probes yielding barcoded amplicons and identification of amplicons through barcod

23 onal profiling (SHAPE-MaP), using ultra-long amplicons and the detection of natural RNA base modifica

24 aired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.

25 hylated or unmethylated CpG sites in the MSP amplicon, and measures the difference in melting tempera

26 circular templates to generate intermediate amplicons, and an off-loop amplification reaction using

27 Amplicons anneal to detection probes that are grafted on

28 We evaluate our multiplexed amplicon approach, PrimalSeq, to demonstrate how virus c

29 ination of metagenomic sequencing and tiling amplicon approaches.

30 Moreover, the sequence and size of each amplicon are also provided for the subsequent experiment

31 causal genes to endocrine resistance in this amplicon are unclear.

32 high-throughput sequencing of 16S rRNA gene amplicons are often preprocessed into composition or rel

33 op amplification reaction using intermediate amplicons as primers for end amplicons.

34 sponsible for the generation of nucleic acid amplicons as RCA products (RCPs).

35 for the 20 SOTR plasma samples, the smaller amplicon assay result was 2.6-fold, 3.4-fold, and 6.5-fo

36 alytical framework, Selection of informative Amplicon Barcodes from Experimental Replicates (SABER),

37 Amplicon barcodes were sequenced from the blood of 56 ad

38 this new approach to identifying informative amplicon barcodes.

39 develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces t

40 differences between taxa, which would affect amplicon based and PCR free methods alike.

41 The amplicon-based deep sequencing method was used in the 3

42 novel viral sequences from data produced by amplicon-based methods.

43 to identify potential off-target sites, and amplicon-based next-generation sequencing (NGS) should b

44 IR genes were typed by using high-resolution amplicon-based next-generation sequencing.

45 NGS techniques to improve the sensitivity of amplicon-based off-target editing quantification.

46 ker) microbial source tracking methods using amplicon-based sequencing of the 16S rRNA gene.

47 r chemically synthesized oligonucleotides or amplicon-based single-stranded DNA probes and validated

48 genic variants were confirmed by a secondary amplicon-based test on cfDNA.

49 We adapted amplicon bisulfite-sequencing protocols to design IMPLIC

50 The sequence-specific recognition of the amplicon by the CRISPR guide RNA and Cas12a enzyme impro

51 The amplicon can be analyzed directly from the amplification

52 The LAMP amplicons can be effectively detected using unmodified S

53 Due to the amplification of probes, amplicons can be visualized with standard epifluorescenc

54 ed metagenomic library, CONKAT-seq evaluates amplicon co-occurrence patterns across library subpools

55 The second round of RCA produces amplicon coils that anneal to detection probes grafted o

56 ss a phytoplankton bloom using 16S rRNA gene amplicon community profiles.

57 n to enrich HBV DNA, generating concatemeric amplicons containing multiple successive copies of the s

58 while maintaining a closed format to prevent amplicon contamination of the workspace.

59 eps that equalize viral load and each type's amplicon copies prior to genotyping by NGS, thereby maxi

60 through analysis of fluorophore-labeled PCR amplicons covering the nuclease target site by capillary

61 , with RNAseq, DNA methylation and bacterial amplicon data spanning its development and range, allows

62 chemical features by analyzing 16S rRNA gene amplicon data through minimum entropy decomposition (MED

63 of samples for which bacterial 16S rRNA gene amplicon data were also available.

64 we developed sensitive, human-specific short-amplicon ddPCR assays targeting repetitive nuclear genom

65 Amplicon deep sequencing (Amp-Seq) offers a tool to addr

66 ed mosquitoes, polymerase chain reaction and amplicon deep sequencing were used to track species and

67 Using qPCR and long-read amplicon deep sequencing, we detected subpopulations wit

68 omet Aquifer, USA, by both 16S rDNA and rRNA amplicon deep sequencing.

69 Through amplicon deep-sequencing placental malaria samples from

70 infection (FOI) and rate of clearance using amplicon deep-sequencing.

71 ses, provided detailed analysis of the ERBB2 amplicon, defined tumor subsets that could benefit from

72 Evaluation of the target amplicons demonstrated that both full-length library (56

73 Julia implementations of Fast Amplicon Denoising (FAD) and Robust Amplicon Denoising (

74 of Fast Amplicon Denoising (FAD) and Robust Amplicon Denoising (RAD), and a webserver interface, are

75 Called 'amplicon denoising', this problem has been extensively s

76 nucleic acid extraction, amplification, and amplicon detection.

77 rs mitotic catastrophe in cells that exhibit amplicon-directed overexpression of TRIM37.

78 using next-generation sequencing of 16S rRNA amplicons directly from amoeba fruiting bodies.

79 a single primer pair, we amplified ~350,000 amplicons distributed throughout the genome.

80 urface cleavage activity of DNAzyme-extended amplicons (DNAzyme-amps) is established, followed by opt

81 signed to measure fluorescence images of the amplicons during a loop-mediated isothermal amplificatio

82 ecific primers found 226bp and 126bp product amplicons for buffalo and cattle meat, respectively.

83 g contact angle), followed by time-dependent amplicon formation and their preferential adsorption to

84 PCR amplified and homo- and hetero-duplexed amplicons formed during the last annealing cycle are sep

85 quencing (NFL-PAS), we generated 733 NFL-PAS amplicons from eight children.

86 Sequences of amplicons from gene-edited parasites exhibited Cas9-cata

87 Sequencing of 16S rRNA gene amplicons from microbiomes harbored in adult males direc

88 In this study, the sequencing of PCR amplicons generated using primers targeting either ketos

89 ned using high-throughput sequencing of rpoB amplicons generated with a Streptomyces specific primer

90 to account for false-negative reactions and amplicon high-resolution melt (HRM) analysis to distingu

91 However, we find no evidence for large amplicons identified in other sex chromosome systems.

92 quantitative real-time PCR and time-resolved amplicon Illumina MiSeq sequencing, we determined a comp

93 Analysis of AA-reconstructed amplicons in a pan-cancer dataset reveals many novel pro

94 llows amplification and sequencing of 2 or 4 amplicons in semi-nested reactions.

95 d generation and sequencing of barcoded mRNA amplicons in situ, directly in fixed cells.

96 analysis (including 14 genes with up to 452 amplicons in total; KRAS, NRAS, HRAS, BRAF, DDR2, ERBB2,

97 The 17q23 amplicon is associated with poor outcome in ER(+) breast

98 The second class of MYCN amplicons is characterized by high structural complexity

99 aceration buffer composition, the target DNA amplicon length, the thermal cycle number and the use of

100 Deep sequencing of amplicon libraries from genomic DNA of gene-edited paras

101 ed NGS approach, 46 M. tuberculosis-specific amplicon libraries had 99.6% (CI 98.0%-99.9%) agreement

102 We also analysed 16S rDNA tag amplicon libraries of the biofilms associated with PAC a

103 oalba and Phocoena phocoena), using 16S rDNA-amplicon metabarcoding.

104 looped template, generating single-stranded amplicon monomers.

105 ng breakage-fusion-bridge cycles, generating amplicons more than 100 megabases in length that became

106 emporal dynamics of transmission, we applied amplicon next-generation sequencing of two polymorphic g

107 Target capture and amplicon NGS demonstrated a high coverage rate of HTNV i

108 le-primer amplification, target capture, and amplicon NGS for whole-genome sequencing of Hantaan orth

109 Furthermore, the amplicon NGS showed a 10-fold (10(2) copies/muL) higher

110 All amplicons of >=9 kb were sequenced and analyzed through

111 cation (NASBA), the assay was able to detect amplicons of 16S rRNA and katG mRNA generated from 0.1 p

112 reads, which are nearly all confined to the amplicons of interest.

113 acterized by using Illumina MiSeq sequencing amplicons of the bacterial 16S ribosomal RNA gene.

114 The amplicons of the two different parasites differ between

115 copy number aberrations, including a notable amplicon on 9p13.

116 e of CTV (CTV-p25) and detect double labeled amplicons on a sandwich immunoassay by designing specifi

117 lysis of the melting temperature (Tm) of the amplicons on qPCR platforms (the Mx3000P qPCR system [St

118 n with an on-chip size-selective trapping of amplicons on silica beads (~8 nL capture chamber) couple

119 stly and difficult to obtain at scale, while amplicon or shotgun metagenomic sequencing data are read

120 The skin microbiota is analysed by 16S amplicon or whole genome sequencing and the skin transcr

121 ration sequencing experiments with PCR-based amplicons or capture-based enrichment systems.

122 ith >70% yield with respect to the input PCR amplicon, or up to 70 pmol per 100 mul PCR reaction.

123 assay to resolve this mixture, despite their amplicons' overlapping T(m)'s in standard EvaGreen melt

124 lex and a fluorescent probe to detect target amplicons produced by standard RT-PCR or isothermal reco

125 Similar accuracy is maintained using amplicon profiles of coral-associated, murine gut, and h

126 d) in intestinal samples were analyzed by16S amplicon profiling.

127 16S rRNA gene amplicon pyrosequencing revealed that bacterial communit

128 degradation measured with the short and long amplicons qPCR, respectively.

129 re, we present an integrity index, the Ratio Amplicon, R(amp) , adapted from human clinical studies,

130 cm and observed that the planktonic/benthic amplicon ratio changed with depth.

131 We then show that proportional indices of amplicon reads capture trends in taxon biomass with high

132 ative proportion of planktonic foraminiferal amplicons remained low from the surface down to 10 cm, l

133 lative proportion of planktonic foraminifera amplicons rocketed, likely reflecting the higher proport

134 ntative samples of both species, and a short amplicon (SA) HRMA was designed based on the presence of

135 16S rRNA amplicon sequence analyses revealed that acetate/lactate

136 Meta-analysis of 16S rRNA amplicon sequence data and metagenome-assembled genomes

137 Single nucleotide exact amplicon sequence variants (ASV) of the human gut microb

138 ese analyses revealed numerous Synechococcus amplicon sequence variants (ASVs) and that clade and ASV

139 Amplicon sequence variants (ASVs) offer a promising alte

140 Almost no (4%) Symbiodiniaceae amplicon sequence variants (ASVs) were found in all thre

141 We identified tens to thousands of amplicon sequence variants (ASVs) within ecotypes, many

142 We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 1

143 ation of the fecal microbiota as read out by amplicon sequence variants (ASVs).

144 cted genetic predisposition using 40 of 3983 amplicon sequence variants as proxies for bacterial spec

145 significant differences in the abundance of amplicon sequence variants between sample types in child

146 We detected 346 fungal amplicon sequence variants dominated by the phyla Ascomy

147 Bacterial amplicon sequence variants of the genera Ralstonia, Pseu

148 We observed 279.84 +/- 0.79 amplicon sequence variants per snail.

149 The majority of EhV-derived SPT amplicon sequences associated with infected cells in the

150 opular naive Bayes taxonomic classifiers for amplicon sequences assume that all species in the refere

151 arbon substrate, with abundant SSU rRNA gene amplicon sequences from hexadecane cultures showing high

152 analyzed over 1.7 billion 16S ribosomal RNA amplicon sequences in the V4 hypervariable region obtain

153 multi-clonal P. falciparum infections, deep amplicon sequencing (AmpSeq) was employed to improve sen

154 Amplicon sequencing (for example, of the 16S rRNA gene)

155 Amplicon sequencing also revealed 27% more families.

156 gnant, n = 39 lactating) using 16S rRNA gene amplicon sequencing and assessed whether the resulting d

157 gate primordial fungal ecology, we performed amplicon sequencing and culture-based techniques of firs

158 to study microbiota composition by 16 S rRNA amplicon sequencing and for short-chain fatty acid (SFCA

159 microbiome was determined by using 16S rRNA amplicon sequencing and fungal load by qPCR.

160 ctions were assessed using 16S ribosomal RNA amplicon sequencing and metagenomic shotgun sequencing.

161 icrobial community structure as evidenced by amplicon sequencing and metagenomics.

162 d metabolites were analyzed by 16S rRNA gene amplicon sequencing and NMR.

163 d the filter microbial community, we applied amplicon sequencing and qPCR targeting key nitrifying gr

164 of the microbiome was determined by 16S rRNA amplicon sequencing and the abundance of taxa described

165 ersatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in singl

166 ing divergences in feed efficiency using 16S amplicon sequencing and untargeted metabolomics.

167 ling suite designed for somatic and germline amplicon sequencing applications.

168 Here, we outline an Illumina amplicon sequencing approach to generate multilocus data

169 We used an amplicon sequencing approach to obtain high-quality geno

170 biome from birth to adulthood using 16S-rRNA amplicon sequencing data and find that the animals share

171 Using high-throughput amplicon sequencing data from the Earth Microbiome Proje

172 prising ~85% of the labeled community in the amplicon sequencing dataset, as closely related to Rhodo

173 ican countries was analysed by 16S rRNA gene amplicon sequencing during the Workshop "Analysis of the

174 Fecal microbiota amplicon sequencing from matched individuals with differ

175 We used Illumina amplicon sequencing fungal assemblages to evaluate effec

176 The 16S rRNA gene amplicon sequencing further showed the co-presence of n-

177 Bisulfite amplicon sequencing has become the primary choice for si

178 16S rRNA gene amplicon sequencing indicated that alterations of the gu

179 In general, eDNA studies that rely on amplicon sequencing may detect hundreds of species prese

180 In this report, the 16S rRNA gene amplicon sequencing method was used to identify bacteria

181 Here we present a high-throughput amplicon sequencing methodology based on PacBio circular

182 Amplicon sequencing of 16S rRNA gene V4 and fungal ITS1

183 adation on transcript community structure by amplicon sequencing of 16S rRNA, amoA and glnA transcrip

184 However, method validation by amplicon sequencing of a MOCK microbial community reveal

185 icrobial profiling for which high-throughput amplicon sequencing of complete genes with single-nucleo

186 Here we used multilocus amplicon sequencing of eDNA to survey biodiversity from

187 High-throughput amplicon sequencing of environmental DNA (eDNA metabarco

188 High-throughput amplicon sequencing of environmental DNA (eDNA metabarco

189 fungal-specific internal transcribed spacer amplicon sequencing of fecal samples.

190 We further used 16S rRNA gene amplicon sequencing of genomic DNA (gDNA) and complement

191 In the metagenome and amplicon sequencing of hgcAB diversity, the Deltaproteob

192 entation using biological data obtained from amplicon sequencing of human skin swab specimens and ora

193 om six Tsimane villages, using 16S rRNA gene amplicon sequencing of longitudinal stool and tongue swa

194 The Illumina MiSeq 16S rRNA gene amplicon sequencing of reactors showed that the microbia

195 Amplicon sequencing of selected samples revealed the pre

196 ied bacterial/viral particle counting, qPCR, amplicon sequencing of the genes encoding 16S and ITS rR

197 Here we used amplicon sequencing of the ribosomal RNA internal transc

198 Publicly available data sets from amplicon sequencing of two 16S ribosomal RNA variable re

199 trawberry growing season using 16S rRNA gene amplicon sequencing on the Illumina MiSeq platform.

200 At family and genera level, 16S rRNA amplicon sequencing results obtained with the low-cost,

201 Amplicon sequencing revealed stark variation in microbia

202 Metagenomics and 16S rRNA gene amplicon sequencing revealed the dominant flanking commu

203 16S rRNA amplicon sequencing showed that the genus Arthrobacter c

204 Long-read next-generation amplicon sequencing shows promise for studying complete

205 We developed a culture-independent amplicon sequencing strategy based on the protein-coding

206 or unusual organisms reported in short-read amplicon sequencing studies and that are not part of the

207 Microbial communities were analysed using amplicon sequencing targeting the bacterial 16S rRNA V3-

208 a farming cohort, were subjected to 16S rRNA amplicon sequencing to characterize bacterial communitie

209 bolomic approach combined with 16S rRNA gene amplicon sequencing to characterize the vaginal microbio

210 rs (Ursus americanus) and used 16S rRNA gene amplicon sequencing to characterize wild black bear GMB

211 study, we use internal transcribed spacer 1 amplicon sequencing to evaluate the salivary mycobiome i

212 Samples were analyzed using 16S amplicon sequencing to identify bacterial profiles acros

213 Microbial samples were analyzed with 16S amplicon sequencing to identify bacterial profiles in yo

214 We used 16S ribosomal RNA gene amplicon sequencing to profile microbiota composition ov

215 ITS2 amplicon sequencing was applied to four families (four m

216 16S rRNA amplicon sequencing was combined with GeoChip metagenomi

217 Amplicon sequencing was integrated with mass balance to

218 Shotgun and 16S ribosomal RNA amplicon sequencing were performed on feces, whereas uri

219 ally new Papillomaviridae sequences from NGS amplicon sequencing with degenerate PV primers.

220 consistent shifts in both the bacterial (16S amplicon sequencing) and fungal (internal transcribed sp

221 d fungal (internal transcribed spacer region amplicon sequencing) communities across replicate lines

222 mplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widel

223 scence in situ hybridization, 16-S rRNA gene amplicon sequencing), yet high-throughput methods for as

224 biota composition, assessed by 16S rRNA gene amplicon sequencing, also differed significantly with ex

225 bial community composition via 16S rRNA gene amplicon sequencing, and functional gene abundance using

226 ta were determined by 16S ribosomal RNA gene amplicon sequencing, and metabolite profiles were analyz

227 anges in microbial community composition via amplicon sequencing, and terrestrial elemental cycling v

228 providing a solution for the analysis of NGS amplicon sequencing, increasingly used in clinical resea

229 ptase-quantitative PCR (RT-Q-PCR) and RT-PCR amplicon sequencing, provide a convenient, target-specif

230 rvariable regions) and 16S rRNA (V1-V3) gene amplicon sequencing, reactors achieved similar and stabl

231 fecal microbiota, evaluated by 16S rRNA gene amplicon sequencing, shifted to a state of reduced bacte

232 ecological community response measured using amplicon sequencing, we combine initial trait data measu

233 In terms of amplicon sequencing, we evaluated five primer sets, two

234 Using ITS1 amplicon sequencing, we examined the fungal portion of t

235 In addition to 16S rRNA gene amplicon sequencing, we performed shotgun metagenomics o

236 It is scalable to thousands of amplicon sequencing-based experiments from any genome ed

237 antified microbial abundances with 16S V3-V4 amplicon sequencing.

238 of field-grown plants using 16S-v4 and ITS1 amplicon sequencing.

239 rough high-throughput 16s ribosomal RNA gene amplicon sequencing.

240 deal resource for SNP genotyping by targeted amplicon sequencing.

241 Microbiota were analyzed by amplicon sequencing.

242 weeks post-term age and analysed by 16S rRNA amplicon sequencing.

243 n Hawaii Island, and analyzed using Illumina amplicon sequencing.

244 the high-throughput nature of 16S rRNA gene amplicon sequencing.

245 ildren and their mothers using 16S rRNA gene amplicon sequencing.

246 ere analyzed by V3-V4 16S ribosomal RNA gene amplicon sequencing.

247 characterized microbiota with 16S rRNA gene amplicon sequencing.

248 microbiota were characterized using 16S rDNA amplicon sequencing.

249 cterized their microbiomes using V4 16S rRNA amplicon sequencing.

250 vaginal microbiota by 16S ribosomal RNA gene amplicon sequencing.

251 entially methylated regions, using bisulfite amplicon sequencing.

252 and Proteobacteria (6.9%), via 16S rRNA gene amplicon sequencing.

253 nity structure determined by next-generation amplicon sequencing.

254 qualitative 16S rRNA gene amplification and amplicon sequencing.

255 19) swards, was characterised using 16S rRNA amplicon sequencing.

256 aeal picoplankton characterized via 16S rRNA amplicon sequencing.

257 Stool samples were collected for microbiota amplicon sequencing.

258 ere sampled and analyzed using 16S rRNA gene amplicon sequencing.

259 y both 16S ribosomal RNA gene and transcript amplicon sequencing; 285 fecal samples were analyzed by

260 and sigmoid colon was determined by 16S rRNA-amplicon-sequencing.

261 IPDA amplicon signal issues resulting from sequence polymorph

262 These findings confirm the impact of amplicon size and target fragmentation on commutability

263 f standards may, in some cases, be linked to amplicon size and the fragmentation of circulating viral

264 suring changes in the signal with respect to amplicon size.

265 oblematic, mostly due to differences in qPCR amplicon sizes between clinical laboratories.

266 oad-range specificity across genera, shorter amplicon sizes that are suitable for use with formalin-f

267 Parameters such as the flow rate of the amplicon solution and trapping time were optimized as we

268 The exact amplicon structure has not been described thus far and t

269 Here, we analyze the MYCN amplicon structure using short-read and Nanopore sequenc

270 pre-trained embeddings, we allow any V4 16S amplicon study to apply the publicly informed properties

271 longation complex P-TEFb - bound to the MYCN-amplicon superenhancer, and its inhibition resulted in s

272 2vec to embed k-mers, obtained from 16S rRNA amplicon surveys, and then leverage an existing sentence

273 taset that was sequenced using Primer ID, an amplicon-tagging protocol, which is designed to reduce e

274 We used amplicon targeted metagenomics to compare microbial comm

275 thanol-responsive polymer generates a tagged amplicon that enables selective precipitation of the mod

276 of persister clones displaying loss of 18q21 amplicons that harbor BCL2.

277 ing barcoded amplicons and identification of amplicons through barcode sequencing.

278 ene (ompA) followed by DNA sequencing of the amplicon to identify the species.

279 Here, we sequenced 16S rRNA gene amplicons to elucidate the attached and suspended prokar

280 okaryote interactions, we used 16S rRNA gene amplicons to evaluate how microbial compositions shift i

281 ol size and remelting curve analysis of qPCR amplicons to monitor changes in pool diversity and seque

282 ying the probes with PCR, and sequencing the amplicons to quantify the expression of chosen genes.

283 ncing of full-length bacterial 16S rRNA gene amplicons was carried out on 21 globally collected speci

284 says followed by bidirectional sequencing of amplicons) was performed on all samples as the reference

285 ences and 9.4 million high-quality 16S V1-V2 amplicons) we identified two deep-branching plastid line

286 sequencing microbial 16S ribosomal RNA gene amplicons, we found that changing climate regimes signif

287 when the two different fluorescently-tagged amplicons were collected magnetically and detected using

288 nge, 2.0 to 11.1 months), 733 single NFL-PAS amplicons were generated.

289 In addition, 18 amplicons were more frequently observed in HR(+)/HER2(-)

290 Amplicons were sequenced (V3-V4 hypervariable region of

291 Internal Transcribed Spacer 1 (ITS1) amplicons were sequenced by Illumina MiSeq.

292 Bacterial V4 16S ribosomal DNA amplicons were sequenced using Illumina MiSeq.

293 cids and functional prediction from 16S gene amplicons were utilized to determine microbiota function

294 This reveals two distinct classes of amplicons which explain the regulatory requirements for

295 PCR detection assay, resulting in an 490 bp amplicon with a consistent melting temperature (T(m) = 8

296 siderably by degenerate primers or targeting amplicons with conserved priming sites.

297 elt curve signatures as compared to 16S rDNA amplicons with enhanced interspecies discrimination.

298 Amplicons with large internal deletions were excluded (<

299 aimed to develop mini-barcode markers (i.e. amplicons with less than 200 bp) suitable for DNA metaba

300 cer samples, we identify a large fraction of amplicons with specific structural signatures suggestive