ライフサイエンスコーパス: anillin

1 emoval and retention of the scaffold protein Anillin.

2 eopontin, PRX1, LMCD1, GPR91, leiomodin, and anillin.

3 a gene involved in cellularization, encodes Anillin.

4 on of APC/C(Cdh1) increased the half-life of anillin.

5 reconstituted by adding an adaptor protein, anillin.

6 phanous and in furrow-progression mutants of Anillin.

7 uler for the recruitment and localization of anillin, a contractile protein and a crucial regulator o

8 We have characterized a human homologue of anillin, a Drosophila actin binding protein.

9 n-independent interaction between Sticky and Anillin, a key scaffold also required for midbody ring f

10 Anillin accumulation at the cleavage furrow is Rho guani

11 recombinant fragments, we determine that the anillin ActBD harbors three distinct actin-binding sites

12 RNA mice, which have reversible knockdown of anillin, actin binding protein (ANLN).

13 d cell biological data suggest that multiple anillin-actin interaction modes promote the faithful pro

14 nd simultaneous knockdown of supervillin and anillin additively increases cell division failure.

15 s in a series of maternal effect and zygotic anillin alleles.

16 intermediate levels of the scaffold protein anillin allowed maximal contraction speed.

17 Anillin also localizes to ectopic cleavage furrows gener

18 Anillin, an actin-binding protein localized at the cleav

19 to the overall F-actin bundling activity of anillin and enables anillin to switch between two actin-

20 Endogenous anillin and Hcdc10 colocalize to punctate foci associate

21 LET-99 acts in a pathway parallel to anillin and is required for myosin enrichment into the c

22 We show that despite their interaction, anillin and myosin II are independently targeted to the

23 at MgcRacGAP is required for the assembly of anillin and myosin into the contractile ring.

24 ity and limit localization of both canonical anillin and non-muscle myosin II (NMM-II) to intercellul

25 his is evidenced by increased RhoA activity, anillin and nonmuscle myosin II in the cytokinetic ring,

26 These results also suggest that anillin and peanut localization are independent of actin

27 for Diaphanous in recruitment of myosin II, anillin and Peanut to the cortical region between actin

28 Two cortical proteins-anillin and RhoA-localized to presumptive abscission sit

29 structures and functional analysis of human anillin and S. pombe Mid1.

30 Cytoskeletal proteins anillin and septin have been found to be responsible for

31 R proteins are required for the retention of anillin and septin in the anterior pole, which are cytok

32 ew, including the myosin II-binding proteins anillin and supervillin, act earlier.

33 Asymmetric ingression requires Anillin and the septins, which promote the coalescence o

34 Anillins and Mid1 are scaffold proteins that play key ro

35 is maintained at the intercellular bridge by anillin, and CYK-4 is localized independently of ZEN-4 b

36 t early and late furrow proteins (Pavarotti, Anillin, and Myosin) are localized to the neuroblast bas

37 epends on the coordinated actions of Sticky, Anillin, and Rho.

38 ocodazole-induced upregulation of cyclin B1, anillin, and securin was decreased in DNA-PKcs-deficient

39 ization of F-actin as controlled by Cofilin, Anillin, and Septin.

40 partially rescued by depleting the canonical Anillin ANI-1 or blocking cytoplasmic streaming.

41 In the absence of both PIG-1/MELK and the anillin ANI-1, myosin accumulates at the anterior cortex

42 rt by limiting the accumulation of canonical anillin ANI-1.

43 rs promote accumulation of the noncannonical anillin ANI-2 at the stable cytoplasmic bridge, which in

44 ate that the cytoskeletal organizing protein anillin (ANI-1) promotes the formation of an aster-direc

45 f three C. elegans proteins with homology to anillin (ANI-1, ANI-2 and ANI-3).

46 hat loss of the cytoskeletal adaptor protein anillin (ANLN) from oligodendrocytes disrupts myelin sep

47 , we identified a missense mutation R431C in anillin (ANLN), an F-actin binding cell cycle gene, as a

48 7A, its substrate Sqh/MRLC, and the effector Anillin/ANLN regulate mitosis and cytokinesis in gliomas

49 Anillins are conserved proteins that are important for s

50 These results reveal anillin as a key regulator of epithelial mechanics and l

51 an in vitro expression screen, we identified anillin as a substrate of the anaphase-promoting complex

52 to recruit actin regulators and the scaffold anillin as well as to regulate RhoA and Rac via its intr

53 Both myosin and anillin assemble into dynamic rho-dependent cortical pat

54 lly bind to RhoA and phospholipids to anchor anillin at the cleavage furrow.

55 of contractile ring components-myosin II and Anillin at the equatorial cortex.

56 -derived Ran-GTP signals that locally reduce Anillin at the growing cell cortex.

57 mechanism, where RhoA activity is limited by anillin binding to the RhoA effector site.

58 al spindle is also disrupted, revealing that anillin can also act at an early stage of cytokinesis.

59 acid changes in the C-terminal PH domain of Anillin cause defects in septin recruitment to the furro

60 video microscopy, we show that three genes: anillin, citron-kinase (CG10522), and soluble N-ethylmal

61 Here, we show that human anillin contains a conserved C-terminal domain that is e

62 Like other metazoan homologs, Drosophila anillin contains a conserved domain that can bind and bu

63 Anillin contains a conserved nuclear localization signal

64 The combined data show anillin contains a cryptic C2 domain and a Rho-binding d

65 We found that the ring component anillin contains a nuclear localization signal (NLS) tha

66 Anillin contains a RhoA-GTP binding domain, which autoin

67 Although anillin contains conserved N-terminal actin and myosin b

68 estigate how the actomyosin scaffold protein anillin contributes to epithelial mechanics in Xenopus l

69 We suggest that anillin-dependent assembly of septin filaments scaffolds

70 LKB1 impinges on myosin via two pathways, an anillin-dependent pathway that also responds to the cull

71 at midcell is achieved through positive Mid1/anillin-dependent signaling emanating from the central n

72 e absence of anillin, furrows cannot form in anillin-depleted cells in which the central spindle is a

73 regulate late steps of maturation including anillin dispersal, ESCRT-III recruitment, and the format

74 The mutant anillin displays reduced binding to the slit diaphragm-a

75 nut, actin, and the actin-associated protein Anillin, do not become correctly localized in pav mutant

76 The nuclear sequestration of anillin during interphase serves to restrict anillin's f

77 d1 and show that duplication of an ancestral anillin early in the Schizosaccharomyces lineage may hav

78 Human cells depleted for anillin fail to properly regulate contraction by myosin

79 esults highlight specialization of divergent anillin family proteins in the C. elegans life cycle and

80 The short Anillin family scaffold protein ANI-2 is enriched at int

81 We found that the levels of anillin fluctuate in the cell cycle, peaking in mitosis

82 SEPT4/SEPT7/SEPT8) and the PI(4,5)P2-adaptor anillin form previously unrecognized filaments that exte

83 Anillin, formins, IQGAPs, and many other proteins regula

84 f the division plane positioning factor Mid1/anillin from the nucleus and negatively by the Pom1/DYRK

85 actin and the septins, likely contribute to anillin function.

86 his is a conserved mechanism for controlling anillin function.

87 We concluded that anillin functions to maintain the localization of active

88 rm and initiate ingression in the absence of anillin, furrows cannot form in anillin-depleted cells i

89 Anillin has an evolutionarily conserved capacity to asso

90 Anillins have been implicated in cytokinesis in several

91 We demonstrate that the anillin homologue Mid1, which dictates correct placement

92 tify and characterize a second fission yeast anillin homologue, Mid2p, which is not orthologous with

93 Retention of the C-terminal NLS in anillin homologues suggests that this is a conserved mec

94 Depletion of anillin in Drosophila or human cultured cells results in

95 We demonstrate upregulation of anillin in podocytes in kidney biopsy specimens from ind

96 These results reveal a novel role for Anillin in regulating epithelial cell-cell junctions.

97 ely, our data point to an important role for Anillin in scaffolding cleavage furrow components, direc

98 We propose a role for anillin in spatially regulating the contractile activity

99 Here, we address the role of anillin in this process and show that anillin inhibits n

100 hat can partly explain the essential role of Anillin in this process.

101 mislocalization of the other, and endogenous anillin increases upon supervillin knockdown.

102 hat also responds to the cullin CUL-5 and an anillin-independent pathway involving the kinase PIG-1/M

103 ole of anillin in this process and show that anillin inhibits not only the accumulation of myosin II

104 -dependent but may also be mediated by known anillin interactions with F-actin and myosin II, which a

105 ne/threonine kinase [8], as a putative novel anillin interactor.

106 emonstrate that the contractile ring protein anillin interacts directly with nonmuscle myosin II and

107 Anillin interacts with Rho, F-actin, and myosin II [3, 8

108 myosin II and actin do not, suggesting that anillin interacts with the septins at the cortex.

109 Anillin is a 124 kDa protein that is highly concentrated

110 These results indicate that anillin is a conserved cleavage furrow component importa

111 Anillin is a conserved component of the contractile ring

112 Anillin is a conserved protein required for cell divisio

113 Anillin is a conserved protein required for cytokinesis

114 Anillin is a conserved scaffold protein involved in orga

115 Anillin is a scaffolding protein that organizes and stab

116 dly showed that junctions recoil faster when anillin is depleted and slower when anillin is overexpre

117 n and myosin II accumulation is reduced when Anillin is depleted.

118 Collectively, these findings suggest that anillin is important in maintaining the integrity of the

119 When Anillin is knocked down, active Rho (Rho-guanosine triph

120 ns and adherens junctions are disrupted when Anillin is knocked down, leading to altered cell shape a

121 ment of vinculin to cell-cell junctions when anillin is overexpressed suggested that anillin promotes

122 ter when anillin is depleted and slower when anillin is overexpressed.

123 We propose that Anillin is required for proper Rho-GTP distribution at c

124 Further, anillin is required to maintain active myosin in the equ

125 Here, we show that anillin is targeted to the nucleus by importin beta2 in

126 suggest the interaction between Rho-GEFs and anillins is an important step in the signaling pathways

127 sed on the regulation of bridge stability by anillins, key regulators of cytokinetic rings and cytopl

128 have investigated embryonic morphogenesis in anillin knockdown (scra RNAi) embryos, where basal cell

129 In anillin knockdown cells, the cleavage furrow ingressed b

130 Additionally, we found that a shorter anillin, known to stabilize bridges [4, 7], also regulat

131 ion-and-rescue assay in Drosophila S2 cells, anillin lacking the entire actin-binding domain (ActBD)

132 which prevents CR assembly in the absence of anillin-like Mid1 and causes CRs to collapse when cytoki

133 ration of the spindle pole bodies (SPB), the anillin-like protein (Mid1p) migrates from the nucleus a

134 otide-exchange factor (RhoGEF) Bud3, and the anillin-like protein Bud4 exclusively at the outer zones

135 e [5-8]; then, massive nuclear export of the anillin-like protein Mid1 at mitosis entry confirms or r

136 control the medial cortical localization of anillin-like protein Mid1 in fission yeast.

137 The anillin-like protein Mid1 localizes to nodes and is requ

138 Cdr1 (also known as Nim1) and Cdr2, and the anillin-like protein Mid1.

139 Under our conditions, the anillin-like protein Mid1p establishes a broad band of s

140 When a fission yeast cell divides, the anillin-like protein mid1p helps to position the contrac

141 a mutation affecting the septin-interacting, anillin-like protein Mid2, suggesting that Scw1 function

142 n important factor in this process is mid1p (anillin-like protein), which is a peripheral-membrane pr

143 sin II and F-actin do contribute, equatorial anillin localization persists in their absence.

144 lay the groundwork for future studies on how anillin may contribute to mechanical events in developme

145 Anillin mediates unilateral furrowing during cytokinesis

146 divide in the middle, only S. pombe uses the anillin Mid1 as a primary nucleus-derived cue to assembl

147 two modules linked by the positional marker anillin Mid1.

148 nchorage of the contractile ring through the anillin/Mid1 family proteins from yeast to humans.

149 ombe, symmetric division is achieved through anillin/Mid1-dependent positive cues released from the c

150 s containing kinase Cdr1p, kinase Cdr2p, and anillin Mid1p form in the cortex around the nucleus earl

151 (cell cycle kinases Cdr1p, Cdr2p, Wee1p, and anillin Mid1p) are constant even when the nodes disassem

152 Anillin Mid1p, Fes/CIP4 homology-Bin/amphiphysin/Rvs (F-

153 G2, mature into cytokinetic nodes by adding anillin Mid1p, myosin-II, formin Cdc12p, and other prote

154 One fission yeast homologue of anillin, Mid1p, is necessary for the proper placement of

155 complexes called nodes whereas cells without Anillin/Mid1p ( mid1) lack visible nodes yet assemble co

156 Gef3 physically interacts with septins and anillin Mid2 and depends on them to localize.

157 also defines the essential roles of a RhoGEF-anillin module in septin architectural remodeling during

158 stral microtubule-dependent pathway requires anillin, NOP-1, and LET-99.

159 itiate central spindle formation, accumulate anillin or actin at the cell equator, or undergo equator

160 pression of a septin-interacting fragment of anillin or by septin depletion via siRNA causes loss of

161 The nuf mutation does not disrupt anillin or peanut recruitment to the metaphase furrows i

162 Disassembly of anillin patches was myosin independent, suggesting that

163 d for disassembly of both myosin patches and anillin patches.

164 We conclude that supervillin and anillin play complementary roles during vertebrate cytok

165 Furthermore, we show that Anillin plays a critical role in regulating cell-cell ju

166 when anillin is overexpressed suggested that anillin promotes junctional tension.

167 the apical surface of epithelial cells, and anillin promotes the tensile forces stored in this netwo

168 dev et al. reveal that the scaffold protein, anillin, promotes asymmetric ring closure by locally seq

169 suggests the conserved function of Mid1-like anillin proteins may be in scaffolding, not positioning,

170 otein nanobody suggests that supervillin and anillin regulate the myosin II and actin cortical cytosk

171 Unifying these findings, we demonstrate that anillin regulates medial-apical actomyosin.

172 ctile ring (CR) through its association with anillin-related Mid1.

173 New work shows the anillin-related protein Mid1 does not position the cytok

174 medial cortical localization and function of anillin-related protein Mid1.

175 Fission yeast cells depend on the anillin-related protein Mid1p for reliable cytokinesis.

176 Ubiquitination of anillin required a destruction-box and was mediated by C

177 ows stable filamentous structures containing anillin, Rho1, and septins to form directly at the equat

178 anillin RNAi caused gradual disruption of the intercellu

179 Mutating the NLS decreases anillin's cortical affinity, causing it to be more domin

180 Finally, we show that anillin's effects on cellular mechanics impact tissue-wi

181 anillin during interphase serves to restrict anillin's function at the cell membrane to mitosis and a

182 ugh importin beta2 binding does not regulate anillin's function in mitosis, it is required to prevent

183 Spatial fine-tuning of anillin's inhibitory role on RhoA signaling enables unil

184 ctive Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes

185 n binding favors a conformation required for anillin's recruitment to the equatorial cortex.

186 The interaction between the anillin's RhoA-binding domain (RBD) and active RhoA is e

187 Anillin's role in scaffolding the membrane cortex with t

188 laevis embryos as a model system to examine Anillin's role in the intact vertebrate epithelium, we f

189 aging-associated diminishment of the septin/anillin-scaffold causes myelin outfoldings that impair t

190 er, reduced nocodazole-induced expression of anillin, securin, and cyclin B1 and phosphorylation of P

191 f four cortical anchoring proteins, RacGAP1, Anillin, SEPT9, and citron kinase (CIT-K).

192 ization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin.

193 ate epithelium, we find that a population of Anillin surprisingly localizes to epithelial cell-cell j

194 To address the mechanism and role of anillin targeting to the nucleus in interphase, we ident

195 utant analysis between scraps, a mutation in anillin that eliminates microfilament rings, and bottlen

196 o contains a potential filament crosslinker, Anillin, that binds all three filament types.

197 Like Drosophila anillin, the human protein localizes to the nucleus duri

198 with an unusual distribution of F-actin and Anillin, these phenotypes are consistent with defective

199 ganized by the SAD-like kinase Cdr2 and Mid1/anillin through an unknown mechanism.

200 n at the cell membrane to mitosis and allows anillin to be rapidly available when the nuclear envelop

201 eptin cytoskeleton acts on the C terminus of Anillin to locally trim away excess membrane from the la

202 tron kinase Sticky acts on the N terminus of Anillin to retain it at the mature MR.

203 hoG signals through the multi-domain protein anillin to stabilize F-actin in these structures.

204 tin bundling activity of anillin and enables anillin to switch between two actin-bundling morphologie

205 1 and CCM-3 are recruited by active RhoA and anillin to the cytokinetic ring, where they in turn limi

206 olleagues report how the scaffolding protein anillin uses cycles of transient binding interactions to

207 Functionally, anillin was required for the completion of cytokinesis.

208 Actin, myosin II, and anillin were all concentrated in these furrows, demonstr

209 Overexpression of Cdh1 reduced the levels of anillin, whereas inactivation of APC/C(Cdh1) increased t

210 red to prevent the cytosolic accumulation of anillin, which disrupts cellular architecture during int

211 Similarly, the essential cytokinetic factor anillin, which functions at the cell membrane to promote

212 contain the COOH-terminal 197 amino acids of anillin, which includes a pleckstrin homology (PH) domai

213 Moreover, Anillin, which is a binding partner of phosphorylated Sq

214 wn of the essential regulator of cytokinesis anillin, which resulted in cytokinesis failure and forma

215 put promotes the simultaneous association of anillin with the plasma membrane, septins, and MTs, inde