ライフサイエンスコーパス: antibody affinity

1 type of hot spot is preferred for increasing antibody affinity.

2 Conjugation had no significant effect on antibody affinity.

3 he role of cooperativity in the evolution of antibody affinity.

4 e a particularly efficient means to increase antibody affinity.

5 individual drug action might be modulated by antibody affinity.

6 ctionalization method that exploit Protein-A/antibody affinity.

7 tion of arginine CDR residues to the overall antibody affinity.

8 gh pH reduces protein adsorption but reduces antibody affinity.

9 nants thereof, such as target expression and antibody affinity.

10 e in germinal centers occurs on the basis of antibody affinity.

11 rties due to the predilection of the antigen-antibody affinity.

12 elated to antigen density, target ratio, and antibody affinity.

13 plasma cell output, T cell interaction, and antibody affinity.

14 the target structure as evidenced by higher antibody affinity.

15 enerate fewer PCs but still of low- and high-antibody affinities.

16 oup of B cell clones bearing a wide range of antibody affinities.

17 methods that rapidly and accurately enhance antibody affinity, addressing issues related to antibody

18 Neutralization activity correlates with antibody affinity against Omicron subvariants BA.1 and B

19 To increase antibody affinity against pathogens, positively selected

20 Antibody affinity against SARS-CoV-2 prefusion spike for

21 In addition, antibody binding and antibody affinity against spike proteins from WA1, BA.1,

22 vaccination the neutralization activity and antibody affinity against the Omicron subvariants is mai

23 These mutations lowered the antibody affinity against the targeting protein and also

24 onse to HIV, somatic mutations that increase antibody affinity also increase breadth and neutralizing

25 of this improvement and explore whether TfR antibody affinity alters the intracellular trafficking o

26 or patients with influenza B is supported by antibody affinity analyses, but confirmation is warrante

27 Antibody affinities and avidities were determined in dir

28 le the two fundamental molecular parameters, antibody affinity and antibody concentration, are challe

29 we observed the correlative patterns between antibody affinity and antigen structure.

30 parameters including bead size, bead number, antibody affinity and assay time, and provide a perspect

31 ltivalent binding provides key insights into antibody affinity and avidity effects and can guide ther

32 hnology leverages significant differences of antibody affinity and chemical reaction rate, which are

33 Both the antibody affinity and complex dissociation rate are impo

34 eaction, during which B cells increase their antibody affinity and differentiate into antibody-secret

35 on how GC cellular and clonal dynamics shape antibody affinity and diversity during the immune respon

36 endent DNA mutator on these genes to improve antibody affinity and effector functions.

37 he constant heavy chain (CH1) domain affects antibody affinity and fine specificity, challenging the

38 Based on antibody affinity and molecular weight, we identified th

39 hese mutations reduce or completely abrogate antibody affinity and neutralising activity, suggesting

40 us metrics that are strongly correlated with antibody affinity and non-specific binding.

41 Despite their pivotal role in defining antibody affinity and protein function, B-hairpins harbo

42 This approach allows for predetermination of antibody affinity and specificity prior to "immunization

43 studies depend on many variables, including antibody affinity and specificity.

44 s, in combination with other factors such as antibody affinity and surface density, contributes to th

45 document any consistent correlation between antibody affinity and the ability to inhibit tumor growt

46 r optimization of cholesterol level, primary antibody affinity, and antibody-bead linkage allowed eff

47 tion, and the effect of various mutations on antibody affinity, and enable a comparison between the b

48 ontributes to shape its epitope specificity, antibody affinity, and functional activities.

49 The changes in antibody affinity are consistent with a transition from

50 Differences in antibody affinity are reflected by differences in the bi

51 ich exosomes on their surfaces using antigen-antibody affinity binding.

52 region (CDR) mutagenesis is used to increase antibody affinity but can be difficult to perform succes

53 es inhibiting anti-Gal is independent of the antibody affinity, but is dependent on the concentration

54 calcium-binding loop rendering the inherent antibody affinity calcium-dependent.

55 entations on patterned surfaces designed for antibody affinity capture.

56 Interestingly, anti-Ibe10R antibody affinity chromatography of HBMEC membrane prote

57 genase digestion to produce NC1 hexamers and antibody affinity chromatography to resolve populations

58 Antibody affinity chromatography was used to fully deple

59 AQP0 C-terminal peptides and AQP0 antibody affinity chromatography were used for affinity

60 photransferase was isolated using monoclonal antibody affinity chromatography, and an alpha2beta2gamm

61 combinant protein in E. coli and purified by antibody affinity chromatography, has hemin binding acti

62 tral salt extraction and by ion exchange and antibody affinity chromatography.

63 exchange chromatography and immobilized bTf antibody affinity chromatography.

64 e, then proceeding with an ACAT-1 monoclonal antibody affinity column and an immobilized metal affini

65 Proteins that co-purified with ABCA1 on an antibody affinity column were identified by liquid chrom

66 purified DMP1-PG with a monoclonal anti-DMP1 antibody affinity column.

67 on system and subsequently purified by a His antibody affinity column.

68 fied as a detergent-stable complex on a CD47 antibody affinity column.

69 as purified from COS cell membranes using an antibody affinity column.

70 Eluted protein from this antibody-affinity column was recognized by antibodies di

71 Measurements of individual antibody affinities coupled with kinetic analysis of equ

72 ng effector cytokines and failed to increase antibody affinity following TIV receipt.

73 is buffer and were not affected by different antibody affinities for their specific epitopes.

74 an, and Fc point mutations greatly increased antibody affinity for and retention on FcgammaRIIIa.

75 Antibody affinity for antigen is believed to govern B ly

76 Antibody affinity for antigen is increased through the a

77 selection in germinal centers (GCs) enhance antibody affinity for antigen.

78 A), and neuraminidase (NA) and also measured antibody affinity for antigenic domains within HA.

79 of E4+ cells had mutations known to increase antibody affinity for Ars, suggesting they were products

80 Antibody affinity for BGAL(266-280) was greater than for

81 Antibody affinity for different F-protein antigenic site

82 otentially pathogenic anti-DNA antibody that antibody affinity for dsDNA does not alone determine the

83 avidity index has no simple relationship to antibody affinity for functional Env spikes on virions.

84 on Western blot, with drastic differences in antibody affinity for these bands.

85 covalent drug-protein adducts; (b) increased antibody affinity: for example, in quinine-type immune t

86 The Env mutations encode antibody affinity gains and select for desired antibody

87 as investigated by performing an antigen and antibody affinity immunoassay.

88 arting with subnanomolar affinity, improving antibody affinity improved cell isolation.

89 ortant function of this system is to improve antibody affinity in an ongoing response; it is turned o

90 The role of antibody affinity in plasma cell (PC) differentiation fr

91 To examine antibody affinity in T cell dependent immune responses,

92 information to develop a strategy to improve antibody affinity in vitro using phage display technolog

93 In a phase 1 malaria vaccine trial, antibody affinity increased following a second vaccinati

94 Increasing antibody affinity into the low picomolar range endowed p

95 Enhancing antibody affinity is a critical goal in antibody design,

96 Antibody affinity is critically important in therapeutic

97 er anti-HBV antibody production is fast, the antibody affinity is high, or the levels of pre-existent

98 For example, antibody affinity is influenced by interactions of frame

99 results presented here demonstrate that the antibody affinity is strongly affected by the presence o

100 he principle of detection and differences in antibody affinity, its positive reference value can be c

101 ed a folded dimeric protein with an enhanced antibody affinity (KD=400 pM).

102 This could be due to different antibody affinities, limited specificity, or distinct ta

103 For certain antibodies, affinity maturation enabled the neutralizati

104 ustained availability of antigens to promote antibody affinity maturation against pathogens and vacci

105 r neutralization activity and cross-reactive antibody affinity maturation against the Omicron BA.1 an

106 ire, antibody kinetics, antibody isotype and antibody affinity maturation against the SARS-CoV-2 pref

107 es on their ability to predict the course of antibody affinity maturation along phylogenetic trees of

108 eir functions in generating antigen-specific antibody affinity maturation and B cell memory.

109 itiates both somatic hypermutation (SHM) for antibody affinity maturation and DNA breakage for antibo

110 antibodies require a deeper understanding of antibody affinity maturation and evolution of the immune

111 vs IgA epitope diversity, antibody binding, antibody affinity maturation and Fc-receptor interaction

112 fter immunization or infection to facilitate antibody affinity maturation and memory and plasma cell

113 lls control antibody responses by supporting antibody affinity maturation and memory formation.

114 antibody-secreting B cells, thus controlling antibody affinity maturation and memory.

115 In addition, RIG-I activation enhanced antibody affinity maturation and plasma cell responses i

116 of immunoglobulin variable regions increases antibody affinity maturation and triggers antibody class

117 roughput screens, biological experiments for antibody affinity maturation are time-consuming and have

118 eting Prmt1 in activated B cells compromises antibody affinity maturation by hampering proliferation

119 Antibody affinity maturation depends on the formation of

120 Antibody affinity maturation enables adaptive immune res

121 Antibody avidity is indicative of antibody affinity maturation following virus infection o

122 on durability of neutralization capacity and antibody affinity maturation generated following two ver

123 In vitro antibody affinity maturation has generally been achieved

124 rgo a dramatic change in phenotype to enable antibody affinity maturation in germinal centers (GCs).

125 omparable to the largest values observed for antibody affinity maturation in vivo or in vitro and ind

126 Antibody affinity maturation involves selective survival

127 Antibody affinity maturation is a critical step in devel

128 n germinal centers and provide evidence that antibody affinity maturation is driven by competition fo

129 Differential antibody affinity maturation is observed against ZIKV st

130 vaccine, OVA loaded NPs demonstrated faster antibody affinity maturation kinetics.

131 an accumulation of LZ GC B cells and reduced antibody affinity maturation likely due to reduced activ

132 Antibody affinity maturation occurs in the germinal cent

133 ithin germinal centers (GCs) enter cycles of antibody affinity maturation or exit the GC as memory ce

134 ological responses with diverse SHM-mediated antibody affinity maturation pathways and divergent epit

135 al B-cell expansions, and also for following antibody affinity maturation processes.

136 By adjusting the antigen-antibody contacts, antibody affinity maturation restricted the potential es

137 s in TIV-induced CD4(+) T-cell expansion and antibody affinity maturation suggests that LypW may pred

138 But whether B cell selection and antibody affinity maturation take place in the face of t

139 ce of identifying key epitopes and utilizing antibody affinity maturation to inform future therapeuti

140 with mild COVID-19 showed marked increase in antibody affinity maturation to prefusion SARS-CoV-2 spi

141 ceptor binding motif and fusion peptide, and antibody affinity maturation to SARS-CoV-2 prefusion spi

142 hese GCs then rapidly regressed and GC-phase antibody affinity maturation was reduced.

143 Class-switch recombination and antibody affinity maturation were also compromised in DK

144 nteraction with helper T cells, and promoted antibody affinity maturation while antagonizing IRF4-dri

145 g de novo antigen recognition and subsequent antibody affinity maturation without initial preimmune B

146 microanatomical sites of B cell mutation and antibody affinity maturation) and related B cell respons

147 Germinal centers (GCs) are the site of antibody affinity maturation, a fundamental immunologica

148 Antibody affinity maturation, a hallmark of adaptive imm

149 Germinal centers (GCs) are the site of antibody affinity maturation, a process that involves co

150 f B cell tolerance seemingly occurred during antibody affinity maturation, an obligatorily T cell-dep

151 ed T cell-dependent antibody production, IgG antibody affinity maturation, and germinal center (GC) f

152 y signal for immunoglobulin class switching, antibody affinity maturation, and priming of CD8+ T-cell

153 o provide the antigenic stimulus that drives antibody affinity maturation, but their role in cellular

154 During antibody affinity maturation, germinal center (GC) B cel

155 In the course of antibody affinity maturation, germinal centre (GC) B cel

156 19) optimize the MBP134 mAb cocktail through antibody affinity maturation, improving its protective e

157 Thus, antibody affinity maturation, including somatic hypermut

158 During antibody affinity maturation, the germinal center (GC) c

159 in antibody variable regions is critical for antibody affinity maturation, with HIV-1 broadly neutral

160 ermline hot spots are effective for in vitro antibody affinity maturation.

161 rminal center (GC) B cells, thereby allowing antibody affinity maturation.

162 ts of the humoral immune response, including antibody affinity maturation.

163 d selection of improbable mutations to guide antibody affinity maturation.

164 nd T cells and required for GC formation and antibody affinity maturation.

165 n, suggests an overall strategy for in vitro antibody affinity maturation.

166 igen-binding affinity, ultimately leading to antibody affinity maturation.

167 tomical sites of B cell clonal expansion and antibody affinity maturation.

168 in those subjects with the highest degree of antibody affinity maturation.

169 (SHM) is the diversity-generating process in antibody affinity maturation.

170 m that strictly limits B cell fitness during antibody affinity maturation.

171 that acquire high-affinity mutations enables antibody affinity maturation.

172 B cell lineages and enhance the outcomes of antibody affinity maturation.

173 h declined rapidly, and induced only minimal antibody affinity maturation.

174 one cells and an inability to undergo robust antibody affinity maturation.

175 he dark zone (DZ) and light zone (LZ) during antibody affinity maturation.

176 l hotspots strategically located to optimize antibody affinity maturation.

177 (SHM) of Ig genes in B cells is crucial for antibody affinity maturation.

178 the phase of proliferative expansion and of antibody affinity maturation.

179 , become germinal centre B cells and undergo antibody affinity maturation.

180 erminal centers (GCs) are important sites of antibody affinity maturation.

181 can be engineered to initiate the process of antibody-affinity maturation against one of the most con

182 an important impact of repeat vaccination on antibody-affinity maturation following vaccination, whic

183 However, antibody-affinity maturation in vivo often fails to prod

184 Antibody-affinity maturation occurs against HA1 domain o

185 ing repeat vaccinations demonstrated reduced antibody-affinity maturation to HA1 of all three influen

186 nd safe immunotherapeutic drugs, fully-human antibodies affinity-matured in a healthy host to a tumor

187 1.8- and 1.7-fold, respectively; and for an antibody affinity of 10(-8) M, increasing t(1/2-I) from

188 s obtained from 10 designs, resulting in the antibody affinity of 2 nM.

189 Even though the effect of antibody affinity on neutralization potency is well docu

190 We describe a process for rapid antibody affinity optimization by repertoire mining to i

191 to motor nerves is not due to differences in antibody affinity or ceramide structural specificity.

192 Increased antibody affinity over time after vaccination, known as

193 of silicon photonic microring resonators for antibody affinity profiling and one-step ricin detection

194 Here we report the antibody affinity purification of a Drosophila TRF2-cont

195 oth human monoclonal anti-DNA antibodies and antibodies affinity purified from the sera of patients w

196 Anti-Neu5Gc antibodies affinity-purified from human serum also direc

197 IgG-enriched fractions and anti-52-kD SSA/Ro antibodies affinity-purified from sera of mothers whose

198 However, injection of anti-Abeta antibodies, affinity-purified from immunized Tg-SwDI mic

199 e compared between intact MVs and monoclonal antibody affinity-purified MV ALP.

200 ed from cleavage of peptide substrates using antibody affinity-purified toxins are detected by MS.

201 JEM, Yada et al. demonstrate that effective antibody affinity selection in germinal centers relies o

202 Antibody affinity selection is also non-homogenizing, in

203 oss-linking followed by positive or negative antibody affinity selection of individual stable conform

204 cell clonal expansion, diversification, and antibody affinity selection.

205 B cells are eliminated during development by antibody-affinity selection and receptor-editing mechani

206 GCs co-mature B cell clones with antibody affinities spanning multiple orders of magnitud

207 To better understand antibody affinity/specificity trade-offs, we have select

208 Most importantly, a significantly higher antibody affinity to RSV G was observed in nasal washes

209 ermore, irrespective of CP treatment, higher antibody affinity to the SARS-CoV-2 prefusion spike was

210 Serum antibody affinity to ZIKV-E protein inversely correlates

211 aggregation and plaque reduction, making the antibody affinity toward Abeta monomer unwanted, as it c

212 ning by affording flexibility through simple antibody affinity variation.

213 Here we delineate the distinct roles of antibody affinity versus avidity in driving these proces

214 Antibody affinity was measured by using a competitive pe

215 s using natural and designed TM domains, and antibody affinity was measured using immobilized and sol

216 To understand the importance of primary antibody affinity, we compared a series of point mutants

217 recognized by HyHEL-63, and their effects on antibody affinity were measured using surface plasmon re

218 ith both procedures, apparent differences in antibody affinity were observed with the new test which

219 C1, and citrullinated C1, together with the antibody affinity, were investigated in OVA-mediated art

220 ving protein-protein docking and engineering antibody affinity, which will help accelerate the develo

221 can accumulate charged residues to increase antibody affinity without compromising specificity.