ライフサイエンスコーパス: antisera

1 determined by PCR and/or agglutination with antisera.

2 itated their escape from killing by anti-Hia antisera.

3 K163 was not highlighted by standard ferret antisera.

4 anti-beta3 integrin, but not anti-GPIbalpha antisera.

5 e index of avidity to the convalescent-phase antisera.

6 could be neutralized by both BTV-1 and BTV-8 antisera.

7 by biochemical tests and agglutination with antisera.

8 ll lines and detection of tubulin forms with antisera.

9 loop of HPV16 L1 induced cross-neutralizing antisera.

10 ositive isolates are serotyped in monovalent antisera.

11 terns seen with the tyramine- and octopamine antisera.

12 ith 61% of trichiasis but only 31% of normal antisera.

13 nt stable trimers recognized by NiV-specific antisera.

14 mouse brain by using two polyclonal anti-NPS antisera.

15 with both anti-PspA/Rx1 and anti-PspA/EF5668 antisera.

16 conventional therapeutics with large-animal antisera.

17 ls in western blot analyses using polyclonal antisera.

18 ments were recognised by both EspA and FliCi antisera.

19 ld be neutralized by posttreatment anti-ROR1 antisera.

20 eptibility to bactericidal activity of mouse antisera.

21 s confirmed by immunoblotting using specific antisera.

22 reactivity with CD3gamma N terminus-specific antisera.

23 usly from complex samples such as polyclonal antisera.

24 A(H3N2) (sH3N2), using postinfection ferret antisera.

25 90s were observed using postinfection ferret antisera.

26 n was highly cross-reactive to TGEV and PRCV antisera.

27 s been hindered by a lack of specific ERbeta antisera.

28 CV), or porcine deltacoronavirus (PDCoV) pig antisera.

29 eduction in HI titers compared to homologous antisera.

30 mined using well-defined rabbit pneumococcal antisera.

31 ocked by incubation with CXCL10-neutralizing antisera.

32 es in common were found using cross-reactive antisera.

33 against strain-specific postinfection ferret antisera.

34 were immunostained with cFos, NPY, and CART antisera.

35 l in cell migration, we tested specific Trbl antisera, a trbl null allele and Trbl transgenes bearing

36 Production of antisera able to recognize individual heterotrimeric G p

37 r interactions than those of the post-immune antisera after immunization of the mixed vaccine.

38 Immunofluorescence analysis with antisera against amino acids 172-193 of Fpn (anti-Fpn 17

39 Antisera against AmOmpA or its predicted binding domain

40 utination (HA) activity was not inhibited by antisera against any of the nine defined APMV serotypes.

41 antiserum against rat dynorphin1-13 but not antisera against beta-endorphin or methionine-enkephalin

42 of PfAMA1 induced parasite growth-inhibitory antisera against both strains of P. falciparum parasites

43 Polyclonal antisera against Bsp22 fully protected epithelial cells

44 Triple immunostaining with antisera against Diploptera punctata allatostatin (Dip-A

45 Antisera against each targeted virus exhibited limited c

46 from stomach were labeled in triplicate with antisera against F4/80, HO1, and CD206, and macrophages

47 Antisera against htt detected a single band broadly migr

48 een 1989 and 2011, and measured HI titers of antisera against influenza A(H3N2) from 24 ferrets again

49 ogy of Fpn was determined using monospecific antisera against its different epitopes, in sheets of ce

50 V GPC monoclonal antibodies (MAbs) and mouse antisera against JUNV or MACV GPC.

51 ty with other BoNTs and is not recognized by antisera against known BoNTs.

52 eparations of the resultant human polyclonal antisera against low- and high-dose VEEV challenges.

53 munocytochemical localization of antipeptide antisera against M5R and the plasmalemmal dopamine trans

54 the poor cross-neutralization of alpha-JUNV antisera against MACV.IMPORTANCE Multiple New World aren

55 moderate-to-severe grade HD patients, using antisera against mitochondrial markers of COX2, SOD2 and

56 ructed and probed for reactivity with ferret antisera against MN/10 and BJ/92 in hemagglutination inh

57 genetic reporter-labeled cells via validated antisera against native CRH.

58 dy we identified neurons immunoreactive with antisera against octopamine, tyramine, and the enzymes r

59 Abl in vitro, we generated affinity-purified antisera against phosphorylated Y251 in Crk and showed t

60 d patterns and their selective labeling with antisera against proteins required for normal mushroom b

61 Using antisera against several of the novel proteins and membr

62 g, we generated and characterized a panel of antisera against subdomains of GP64.

63 We raised antisera against synthetic peptides corresponding to an

64 Cpn and Ctr peptide antigens react only with antisera against the cognate chlamydial species.

65 hot plate test is sensitive to antagonism by antisera against the endogenous opioid peptide beta-endo

66 Antisera against the intracellular, C-termini of divalen

67 nths were stained immunohistochemically with antisera against the pan-neuronal marker neurotubulin.

68 Immunoprecipitation with antisera against the proteins revealed a strong interact

69 To confirm this, rabbit antisera against the putative capsid proteins of TTSuV1a

70 Rabbit antisera against this recombinant vaccine cross-reacted

71 These new antisera, alongside other specific antibodies, labeled s

72 Antisera also inhibited other mAbs using the same variab

73 Different antisera also recognized other glycan determinants, incl

74 Both antisera always stained XMRV-infected or -transfected ce

75 by CCIF between TGEV Miller hyperimmune pig antisera and all PEDV strains.

76 n inhibition (HI) data with monovalent swine antisera and antigenic cartography to evaluate the antig

77 absorbed neutralizing activity from complex antisera and b12.

78 and 7 (1.8%) isolates were nontypeable with antisera and by mPCR/RLB, respectively; and 45/56 (80.4%

79 ization density, whereas passive transfer of antisera and CD4(+) T cells was needed to cause a signif

80 relocalization, and using a phospho-specific antisera and genetic manipulations, identify where Warts

81 tious virus that was neutralized by specific antisera and had a mutation rate similar to conventional

82 cross-reactivity between the virus-specific antisera and heterologous filovirus antigens.

83 inhibited only by murine anti-beta3 integrin antisera and human anti-HPA-1a IgG purified from mothers

84 ing caused by anti-keratin antibodies in the antisera and keratin contamination of reagents.

85 abilized microtubules using a combination of antisera and mass spectrometry to detect the products.

86 that immunization-induced polyclonal animal antisera and monoclonal antibodies specific for either C

87 d CT709 together reacting with 46% of normal antisera and none from the trichiasis group, whereas ant

88 d neutralization reduction against patients' antisera and substantially increased virus binding abili

89 well as by inhibition of binding between the antisera and the O-SPs of both bacteria.

90 herein termed EBOTAb, was prepared from the antisera and used for an in vivo guinea pig study.

91 vel iron compound receptor, reacted with the antisera and were identified by mass spectrometry.

92 gens were recognized by at least 1 of the 59 antisera, and 10 antigens by 50% or more antisera, the l

93 e requires no cell-sorting, cross-linking or antisera, and binding profiles can be generated from as

94 -neutralizing antibodies in mouse and ferret antisera, and their ability to replicate in mice, we hav

95 a mechanism for the cross-neutralizing human antisera: antibodies present in the antisera bind to con

96 By generating FcRLA-specific antisera as well as knockout mice, we were able to unequ

97 pite strong cross-reactivity of convalescent antisera between related arenavirus species, weak or no

98 ng human antisera: antibodies present in the antisera bind to conserved regions associated with cross

99 MV fusion machinery and, in conjunction with antisera binding studies, provide a rationale for how in

100 frared intensity of IR-dye labeled secondary antisera bound to targeted proteins of the MAPK- and cAM

101 collectively reacting with 30% of trichiasis antisera but none from the normal group, and antigen CT6

102 Here, we generate filovirus-specific antisera by prime-boost immunization of groups of captiv

103 Although reciprocally reactive with antisera by Western blotting, this truncated protein did

104 cted by the limited availability of specific antisera, by difficulties discriminating the discrete lo

105 Passive administration of serotype-specific antisera capable of opsonizing and killing S. pneumoniae

106 uble labeling studies with YFP and serotonin antisera combined with electron microscopy were carried

107 were detected in the elicited sera, and the antisera competed with b12 for CD4bs-directed binding sp

108 wing a gp120 DNA vaccine prime) and that the antisera competed with bNAbs from 3 classes of nonoverla

109 Neutralization by L1 antisera confirmed that infectious transmission was caps

110 Western blot analysis, using LTB antisera, confirmed the presence of a 17-KDa protein in

111 In hemagglutination inhibition assays, 12 antisera cross-reacted with >/=14 of 20 H10 viruses, and

112 ecipitation experiments using anti-puromycin antisera demonstrated that the majority of peptidyl-puro

113 -to-head comparison between ferret and human antisera derived antigenic maps revealed different antig

114 typic challenge compared to that provided by antisera derived from mice immunized with rAdH5 or rAdM2

115 Anti-polyglutamine antisera detected full-length mutant htt in HD brain.

116 tested H5N1 viruses (and clade 2.2-specific antisera detected most of the H5N1 antigens), ancestral

117 Ferret antisera detected no or little cross-reactivity between

118 Ferret antisera detected no or little cross-reactivity between

119 th lipopolysaccharide (LPS) or nephritogenic antisera developed markedly less proteinuria than did co

120 Primary infection antisera did not neutralize all viruses of the same DENV

121 uropeptides provided the first evidence that antisera directed against calcitonin gene-related peptid

122 f FLNa in the mature rat brain, by using two antisera directed against epitopes at either the N' or t

123 Additional studies of antisera directed against small subdomains revealed that

124 We generated polyclonal antisera directed against two of the predicted replicase

125 he glycan microarray analysis of FMS-induced antisera displayed a high specificity toward tumor-assoc

126 Moreover, vaccination-induced antisera displayed strong angiostatic and tumor-growth-i

127 The IHC used 2 rabbit polyclonal antisera, each prepared against a purified murine leukem

128 primary HIV-1 sensitive to neutralization by antisera elicited by immunization of rabbits with HIV-1

129 nation inhibition (HAI) antibody titers than antisera elicited by VLP vaccines with wild-type HA VLPs

130 beta1 integrin-neutralizing antisera erased LTP when applied during, but not after,

131 agonism, but were eliminated by neutralizing antisera for beta1-integrins.

132 antibodies may be a useful adjunct to ferret antisera for detecting antigenic drift in influenza viru

133 of hemagglutination by postinfection ferret antisera for many viruses isolated in MDCK cells, includ

134 gel electrophoresis and probed using pooled antisera from 20 CBA/J mice chronically infected with E.

135 When antisera from 33 trichiasis and 26 control patients (wit

136 ydial proteins was recognized by one or more antisera from 99 women urogenitally infected with C. tra

137 Antisera from both wild-type and MHC-II-deficient mice p

138 h American isolates (clade 2.3.4.4), whereas antisera from dk/Hok/69 ca-vaccinated ferrets cross-reac

139 Antisera from Fc-d E1E2-immunized mice exhibited stronge

140 Furthermore, antisera from five immunized human vaccinees were shown

141 pping of anti-E1E2 antibodies present within antisera from goats and humans immunized with HCV-1 rE1E

142 Antisera from guinea pigs immunized with the PMD-modifie

143 We produced purified equine antisera from horses hyperimmunized with EBOV virus-like

144 ed immunosorbent assays (ELISAs) showed that antisera from immunized mice inhibited monoclonal antibo

145 Antisera from immunized mice showed that Fc-d E1E2 elici

146 s optimized using experimental and reference antisera from immunized, convalescent, and naive animals

147 GII.17 cluster IIIb VLP was blocked only by antisera from mice immunized with cluster IIIb VLPs.

148 emagglutinin inhibition antibody titers with antisera from mice infected with wild-type or glycan mut

149 Six monoclonal antibodies (mAbs) and antisera from mice specific for PA were tested for their

150 ally, AAVhum.8 can better evade neutralizing antisera from multiple species than AAV8.

151 Antisera from rabbits actively immunized with PPSV23 and

152 sequential epitopes identified in polyclonal antisera from recombinant LF-immunized mice.

153 esponses was evaluated in ELISA format using antisera from sheep and cattle experimentally infected w

154 Antisera from the 40 mice recognized 130 out of 257 C. m

155 lar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, Califor

156 ssays to show that antibodies present in the antisera from vaccinated goats and humans bind epitopes

157 Both antisera gave the same distinctive immunofluorescent sta

158 /2014 (H5N6) virus was developed, and ferret antisera generated against this virus were demonstrated

159 Administration of antisera generated in mice and rabbits by immunization w

160 Antisera generated to individual H1.2/H1.4 interphase ph

161 DBPII, which is strain transcending, whereas antisera generated to SD1+2 and SD2 failed to generate b

162 The elicited antisera had the ability to neutralize virus infection a

163 Screening of these proteins against ovine antisera identified eight immunogenic proteins that are

164 ein microarray results, and phospho-specific antisera identified serine 189, within the Erk3 activati

165 and tested the post-exposure efficacy of the antisera in a mouse model of infection.

166 her, viruses possessing this mutation escape antisera in HAI assays simply by attaching to cells more

167 ming the problem of availability of suitable antisera in testing laboratories as well as facilitating

168 on of the neutralizing activity of the ovine antisera in vitro revealed that it neutralized EBOV.

169 Consistently, adoptive transfer of antisera induced by rAdH5/M2e provided the best protecti

170 CLEC14A antisera inhibited cell migration and tube formation, su

171 More stringent selection with pooled human antisera (intravenous immunoglobulin [IVIG]) then led to

172 -CoV-2 by spike-specific antibodies in these antisera is highly correlated.

173 SAT2 capsid to broaden cross-reactivity with antisera is provided.

174 RNA in situ hybridization and anti-Black antisera localized Black expression in the head.

175 Some vaccine antisera mediated bactericidal activity against a N. men

176 Immunohistochemical analyses with TSPO antisera, methoxy-X04 staining for fibrillary beta-amylo

177 igen loss, we report that, unlike polyclonal antisera, monoclonal antibodies did not induce antigen l

178 se existing detoxification platforms such as antisera, monoclonal antibodies, small-molecule inhibito

179 None of the pig TGEV antisera neutralized PEDV and vice versa.

180 from other influenza strains, compared with antisera obtained with unmodified HA trimers.

181 that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recog

182 The antisera of both conjugates showed strong binding and me

183 ization activity against humoral immunity in antisera of patients and healthy adults and greatly incr

184 In addition, the mouse post-immune antisera of the fused vaccine revealed significantly hig

185 g reaction using a new and expanded group of antisera, optochin susceptibility and bile solubility te

186 Passive immunization with Hla-specific antisera or active immunization with a nontoxigenic form

187 In infant mice, passive transfer of antisera or CD4(+) T cells from PhtD-vaccinated adult mi

188 Guinea pigs given 20 mg of antisera or F(ab')2 at or starting at 1 or 2 dpi were al

189 Serotype-specific rabbit polyclonal antisera or mock antisera were passively administered to

190 her epitopes identified using the polyclonal antisera overlap binding sites for previously reported m

191 ice were given up to 2 mg of purified equine antisera per animal, at 30 minutes, 1 or 2 days post-inf

192 Overall, Denka Seiken commercial antisera performed very well and, together with the comp

193 est sequence homology, we tested hyperimmune antisera prepared for each virus against baculovirus-exp

194 Antisera produced against recombinant albicin recognized

195 igs at fairs did not cross-react with ferret antisera produced against the human seasonal H3N2 IAVs c

196 ein CT795 was dominantly recognized by human antisera produced during C. trachomatis infection but no

197 Furthermore, TcpF antisera protect against V. cholerae infection.

198 found no evidence that high-titer antipilin antisera provided passive protection against a sublethal

199 Antisera raised against an H3N2 strain possessing K145 h

200 g C. trachomatis infection but not by animal antisera raised against dead chlamydia organisms.

201 antigen displaying reduced reactivity toward antisera raised against the native R. terrigena repeat s

202 utralization assays using convalescent-phase antisera raised against the parental virus, SAT2/ZIM/7/8

203 -qPCR, and immunohistochemistry with several antisera raised against TRPML3 to determine the expressi

204 cells, which nonspecifically react with two antisera raised against TRPML3.

205 Antisera raised in laboratory animals against SD3, SD3+,

206 exneri can be phenotypically serotyped using antisera raised to type-specific somatic antigens and gr

207 The antisera reacted equally well with the LPSs of both bact

208 We also found that these antisera reacted with flagellae of Pseudomonas syringae,

209 Importantly, the mice antisera recognize cancer cells in biopsies of breast ca

210 d the electron microscopic immunolabeling of antisera recognizing CRF or CRFr.

211 icity of LT-IIc was partially neutralized by antisera recognizing LT-IIa or LT-IIb but not by anti-CT

212 Finally, the passive transfer of anti-NEAT antisera reduced mortality and disease severity, suggest

213 ted with 35% of normal and 19% of trichiasis antisera respectively.

214 nhibition (HI) titer with ferret and chicken antisera, respectively.

215 Limited availability of antisera resulted in poor typeability values from the fo

216 The labeling patterns of two GnRH antisera revealed different populations of GnRH-immunore

217 nity selection on rabbit anti-NoV polyclonal antisera revealed five families of overlapping sequences

218 Nischarin, or imidazoline receptor antisera-selected protein, is a protein involved in a va

219 Neutralizing antisera selective for beta1 integrins blocked these eff

220 e E. coli chromosome, restoring native level antisera sensitivity and structure.

221 otein gene and immunoreactivity to flotillin antisera served as respective physical, molecular and bi

222 The resulting mouse and rabbit antisera showed complement-mediated bactericidal activit

223 Antisera showed the best competition against HC84.26 and

224 he altruronic acid-containing polysaccharide antisera showing the greatest opsonophagocytic assay act

225 Pooled antisera significantly reduced pathogenic activity from

226 Immunohistochemical analysis with antisera specific for deer tick virus identified numerou

227 Passive immunizations with antisera targeting outer membrane proteins (OMPs) have s

228 RVPs were better neutralized by heterologous antisera than A1-160K.

229 f cross-reactivity against heterogeneous VLP antisera than the parental VLPs.

230 evels of cross-reactivities to heterogeneous antisera than the parental VLPs.

231 ive transfer experiments at high viral dose, antisera that fully protects wild-type mice fails to pro

232 lian TGF-beta did not recognize HES, whereas antisera that inhibited HES did not affect TGF-beta.

233 Using monospecific polyclonal peptide antisera that recognize only cHsp60-1, cHsp60-2, or cHsp

234 n 1 of FVO and domain 2 + 3 of 3D7 generated antisera that showed similarly high level inhibition of

235 op causing interference, mouse anti-JUNV GPC antisera that solely neutralized pseudovirions bearing a

236 p A M peptides that were predicted to elicit antisera that would cross-react with an additional 15 no

237 viruses were neutralized only by homologous antisera, the chimeric proteins could be neutralized by

238 59 antisera, and 10 antigens by 50% or more antisera, the latter being designated as immunodominant

239 en of the 719 Ags were recognized by >or=50% antisera, thus designated as immunodominant Ags.

240 e of complement and polymorphonuclear cells, antisera to 9Glc-NH(2)-TT mediated the killing of A. bau

241 infection in mice, pneumonia and bacteremia, antisera to 9Glc-NH(2)-TT significantly reduced levels o

242 Further, rabbit antisera to alpha11-88x8 and alpha11-88x5 similarly neut

243 SA) and Western blot analysis using specific antisera to animal CoVs and SARS-CoV and SARS patient co

244 nt comprising aa 1 to 213 cross-reacted with antisera to animal CoVs, whereas the fragment comprising

245 found that, compared to normal rabbit sera, antisera to Ata significantly reduced the levels of A. b

246 Moreover, the TRIM21-mediated ability of antisera to block replication was a consistent feature o

247 Blockade of CIRP using antisera to CIRP attenuated inflammatory cytokine releas

248 ndividual vaccine showed efficacy, combining antisera to CP antigens and PNAG still abrogated individ

249 Antisera to DBL4epsilon and to DBL5epsilon inhibit mater

250 Studies with antisera to different epitopes of Fpn indicate that the

251 e used double-label immunocytochemistry with antisera to different isoforms of GnRH and to choline ac

252 within L1-epitome were recognized by murine antisera to HPV58 virus-like particles, suggesting that

253 Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbia

254 c axons in myenteric ganglia were labeled by antisera to one of these eight markers.

255 Here, using a panel of antisera to phosphorylated and nonphosphorylated sites o

256 Antisera to PNAG mediated opsonophagocytic killing of BC

257 s, these capsids were seen to be reactive to antisera to pORF65 as well as to KSHV-positive human ser

258 Antisera to rBCP, rBCP(DeltaIgA), III-rBCP, and III-rBCP

259 induced high titre antibody responses, only antisera to rDn-ACP-QuilA and rDn-ACP-Al(OH)(3) signific

260 Antisera to recombinant p30 expressed in Escherichia col

261 pes exposed in all VAR2CSA alleles; however, antisera to several DBL domains cross-reacted on parasit

262 Antisera to SREBP-1 were used with liver chromatin from

263 t to and infection of Caco-2A cells, whereas antisera to the recombinant protein also inhibit infecti

264 pped on E6, E7 and L1 proteins using rabbits antisera to the respective recombinant proteins.

265 Passive immunization with antisera to these antigens is effective in reducing clin

266 Mouse antisera to these five proteins inhibited binding of GBS

267 We generated antisera to this carboxyl terminus and identified immuno

268 differentiated O1(+) oligodendrocytes, while antisera to TIMP-1 reduced oligodendrocyte numbers.

269 complexes followed by Western blotting using antisera to various FAO enzymes.

270 globulins and increasing the toxicity of the antisera toward cancer cells.

271 abetic human pancreas is challenging because antisera used for detection of the GLP-1 receptor often

272 Seiken (Tokyo, Japan) somatic and flagellar antisera (using a tube agglutination assay) were 94.0% a

273 The elicited antisera was assessed for hemagglutination inhibition ac

274 ralization potency of Apobec3/Rfv3-resistant antisera was dependent on activating Fcgamma receptors b

275 he possibility of serum sickness, the equine antisera was digested with pepsin to generate F(ab')2 fr

276 Passive transfer of alpha11-88x8 antisera was protective.

277 Immunostaining with dopamine and TH antisera was used to corroborate targeted transgene expr

278 Using specific antisera, we demonstrate that Rpl38 is an approximately

279 Using antisera, we determined the localization and gross abund

280 By testing with 185 human antisera, we expanded 18 previously discovered C. pneumo

281 Using ferret antisera, we have established the feasibility of measuri

282 Human vaccine sera and ferret antisera were analyzed by hemagglutination inhibition (H

283 Human vaccine sera and ferret antisera were analyzed by hemagglutination inhibition (H

284 Conjugate-induced antisera were bactericidal against B. pertussis, and the

285 Eight subdomain-specific antisera were generated, and their reactivities with GP6

286 Antisera were initially tested against laboratory lines

287 grees of cross-reactivity; Egy/09 ca vaccine antisera were more specific for the gs/GD lineage viruse

288 -specific rabbit polyclonal antisera or mock antisera were passively administered to rabbits before e

289 Ferret reference antisera were raised using clinical specimens containing

290 The antisera were reciprocally reactive with both proteins.

291 Polyclonal rabbit antisera were screened against the coating antigen libra

292 The rabbit antisera were screened and the combination of antiserum

293 Human and ferret antisera were tested in HI assays against 1 representati

294 When Denka Seiken flagellar antisera were used in a slide agglutination assay, the s

295 llected in 2007 and previously serotyped via antisera, were screened with the assay.

296 binding approaches and the lack of specific antisera, which have supported immunolocalizations at od

297 hat would be expected from primary infection antisera, while 11 sera had lower, more dispersed patter

298 ss-reactivity to TGEV Miller and TGEV Purdue antisera, while N protein presented some cross-reactivit

299 vity (using anti-raw and anti-roasted walnut antisera), with similar patterns of reactivity observed

300 LR-LO10, which has low reactivity with human antisera, yet has high cytotoxic and antitumor activity