ライフサイエンスコーパス: argonaute

1 ssing cofactors, including DROSHA, PASHA, or Argonaute.

2 f programmable gene-silencing proteins named Argonaute.

3 leosides 10 and 12, at the catalytic site of Argonaute.

4 hubs for self/non-self RNA discrimination by Argonautes.

5 e 5'-phosphorylated guides used by all known Argonautes.

6 restrict or enable interaction with specific Argonautes.

7 cterial Argonautes much more than eukaryotic Argonautes.

8 hat of RNA interference-mediating eukaryotic Argonautes.

9 s by gating small RNAs into specific nuclear Argonautes.

10 also measured the association of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the

11 the DNA DAMAGE-BINDING PROTEIN 2 (DDB2) and ARGONAUTE 1 (AGO1) of Arabidopsis thaliana form a chroma

12 is likely to involve the RNA-binding protein Argonaute 1 (AGO1), as raw and AGO1 genetically interact

13 We previously identified Argonaute 1 (AGO1; a key component of microRNA-induced s

14 Mosquito Loqs interacted with both argonaute 1 and 2 in a manner independent of its interac

15 litating the assembly of the microRNA-guided Argonaute 1 complex and gene silencing.

16 ed at 72 h posteclosion and 24 h PBM through Argonaute 1 cross-linking and immunoprecipitation follow

17 omplex between the paRNA and microRNA-guided Argonaute 1 that, together, recruit SUV39H1 and induce r

18 ticipated in angiogenesis by targeting AGO1 (argonaute 1) and upregulating VEGF (vascular endothelial

19 Argonaute 1-4, glycolytic enzymes, and cytoskeletal prot

20 of RNA-DEPENDENT RNA POLYMERASE 6 (RDR6) to ARGONAUTE 1-derived cleavage products, resulting in more

21 ost RNA-interference machinery that involves ARGONAUTE 1.

22 Argonaute 2 (AGO2) and miR-17 binding were essential for

23 crosslinked and immunoprecipitated with anti-Argonaute 2 (Ago2) antibody.

24 rom RNA-induced silencing complex (RISC) and Argonaute 2 (Ago2) associated with therapeutic siRNA.

25 rs to mature Sal-1 is dependent on host cell Argonaute 2 (AGO2) but not Dicer.

26 iation of miRNAs with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) to assess the functional status of in

27 creatic cancer development and interact with Argonaute 2 (AGO2) to perturb its function.

28 C virus (HCV) RNA genomes by recruiting host argonaute 2 (AGO2) to the 5' end and preventing decay me

29 novel mechanism whereby target engagement by Argonaute 2 (AGO2) triggers its hierarchical, multi-site

30 nding cofactor of APOBEC1 in RNA editing, or Argonaute 2 (AGO2), a key factor in the biogenesis of ce

31 Argonaute 2 (Ago2), a member of the miRNA silencing comp

32 a2 ceRNA through differential recruitment to Argonaute 2 (Ago2), and TGF-beta signalling driven by Tg

33 shown that AUF1 facilitates miRNA loading to Argonaute 2 (AGO2), the catalytic component of the RNA-i

34 ealed that AUF1 promoted let-7b loading onto Argonaute 2 (AGO2), the catalytic component of the RNA-i

35 te conserved and S-nitrosylated in mammalian Argonaute 2 (AGO2)-alters its function in controlling ge

36 -dependent manner via classic recruitment of Argonaute 2 (AGO2).

37 reassembling complexes with the human enzyme Argonaute 2 (hAgo2) and Cryptosporidium single-stranded

38 2.3 A resolution crystal structure of human Argonaute 2 (hAgo2), a key nuclease in the RNAi pathway,

39 1 suppresses its transcription by recruiting Argonaute 2 and inhibiting RNA polymerase II binding.

40 proteins, including Fem-3-binding-factor 2, Argonaute 2 and Ribonuclease III, NucleicNet can accurat

41 alized by endothelial cells, where the miRNA-Argonaute 2 complexes modulate target gene expression an

42 trate that such EVs contain functional miRNA-Argonaute 2 complexes that are derived from the host RBC

43 ed, vsiRNAs were not efficiently loaded into Argonaute 2 during wild-type IIV6 infection.

44 Moreover, Argonaute 2 immunoprecipitation sequencing, qRT-PCR, and

45 (PAR-CLIP), we isolated RNAs associated with Argonaute 2 in the RNA-induced silencing complex (RISC)

46 also from other organisms such as the human Argonaute 2 mitochondrial echoform.

47 d and supplementary helix fitting into human Argonaute 2 protein (Ago2), reminiscent of an active sta

48 compartments and loaded into newly generated Argonaute 2 protein complexes weeks after dosing, enabli

49 n, and miR-3189-3p coimmunoprecipitated with Argonaute 2 together with two of its major predicted gen

50 Inhibition of DROSHA or Argonaute 2, or disruption of microRNA recognition eleme

51 a robust RNAi pathway that links to TGS via Argonaute 2-2 (Ago2-2) associated 27-nucleotide small RN

52 A comprehensive identification of Argonaute 2-associated microRNAs and mRNAs in endothelia

53 initiated the decay of both protein-free and Argonaute 2-loaded miRNAs via endonucleolytic cleavage a

54 IP1 in the GW-body, which features GW182 and Argonaute 2.

55 s reduced but not eliminated by knockdown of argonaute 2.

56 le-stranded antisense RNA coupled with human Argonaute 2.

57 Supporting this, Argonaute-2 (Ago-2), the core component of miRISC, can c

58 ociation with HDL or the RNA-binding protein argonaute-2 (Ago-2).

59 h RNA-induced silencing complex constituents argonaute-2 (Ago2) and fragile X mental retardation-rela

60 Argonaute-2 (Ago2) is a key component of the RNA-induced

61 olecule FRET to directly visualize how human Argonaute-2 (Ago2) searches for and identifies target si

62 these modifications impact interactions with Argonaute-2 (Ago2), the molecular target of siRNAs, is n

63 and instead relies on the slicer activity of Argonaute-2 (Ago2).

64 ARGONAUTE-2 and associated miRNAs form the RNA-induced s

65 ve determined the crystal structure of human Argonaute-2 in complex with the metabolically stable 5-(

66 inity of the 5-E-VP -modified siRNA to human Argonaute-2 in-vitro, as well as the enhanced silencing

67 he 5 binding site in the Mid domain of human Argonaute-2 is able to adjust the key residues in the 5-

68 3'-Untranslated region reporter assays, argonaute-2 microribonucleoprotein immunoprecipitation s

69 Argonaute-2 protein (Ago2), a major component of RNA-ind

70 RNA processing machinery including Dicer and Argonaute-2, which allow for cell-free pre-miRNA process

71 sferase 1 (PNPT1) and has higher affinity to Argonaute-2, which may contribute to its higher stabilit

72 Aubergine (Aub), Argonaute 3 (Ago3), and components of the nuclear RDC co

73 hila, two Piwi proteins, Aubergine (Aub) and Argonaute-3 (Ago3), localize to perinuclear "nuage" gran

74 urvival and viral titers of Piwi, Aubergine, Argonaute-3, and Zucchini mutant flies were similar to t

75 de novo DNA methylation by interacting with ARGONAUTE 4 (AGO4) and by controlling the local abundanc

76 r-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4).

77 enes such as the RNA silencing effector gene ARGONAUTE 5 (AGO5).

78 We highlight Argonaute 9 (AGO9) and Argonaute 6 (AGO6) as candidate binding partners of prem

79 TE mRNAs are directly incorporated into the ARGONAUTE 6 (AGO6) protein and direct AGO6 to TE chromat

80 We highlight Argonaute 9 (AGO9) and Argonaute 6 (AGO6) as candidate b

81 HITS-CLIP revealed hundreds of robust Argonaute (Ago) binding sites mediated by miR-HSURs that

82 ecipitation (HITS-CLIP) experiments of human Argonaute (AGO) during HCV infection showed robust AGO b

83 the mechanism of FMRP's role in suppressing Argonaute (AGO) family members' association with mRNAs b

84 Argonaute (AGO) family proteins are conserved key compon

85 This feature indicates that the miRNA-Argonaute (AGO) machinery is ancient and the primary fun

86 Of the four human Argonaute (AGO) paralogs, only AGO2 has been shown to ha

87 At the RISC core is one Argonaute (AGO) protein that, guided by endogenous or vi

88 and ligation of miRNA-target chimeras on the Argonaute (AGO) protein to globally map miRNA interactio

89 specificity to guide the silencing effector Argonaute (AGO) protein to target mRNAs via a base-pairi

90 he tiny regulatory RNAs, form complexes with Argonaute (Ago) proteins and inhibit gene expression in

91 interact with members of the same family of Argonaute (Ago) proteins and their function in target re

92 Argonaute (AGO) proteins are a well-conserved multigene

93 Argonaute (AGO) proteins are core components of RNA inte

94 Argonaute (Ago) proteins are important effectors in RNA

95 Argonaute (Ago) proteins are key players in both gene re

96 Argonaute (Ago) proteins are key players in RNA interfer

97 As effectors in the RNA silencing pathway, ARGONAUTE (AGO) proteins are targeted by some VSRs, such

98 ARGONAUTE (AGO) proteins associate with small RNAs in vi

99 ARGONAUTE (AGO) proteins bind directly to miRNAs and may

100 The Argonaute (AGO) proteins for loading small RNAs are dupl

101 In mammals, argonaute (AGO) proteins have been characterized for the

102 Small RNAs (sRNAs) associate with Argonaute (AGO) proteins in effector complexes, termed R

103 Argonaute (Ago) proteins in eukaryotes are known as key

104 trons, are associated with and stabilized by Argonaute (Ago) proteins in the cytoplasm.

105 ring microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the T

106 Argonaute (AGO) proteins recruit 21-24-nucleotide (nt) s

107 be inhibited by VSRs, small RNAs (sRNAs) and Argonaute (AGO) proteins seem to be the most frequent ta

108 osslinking immunoprecipitation (CLIP) of the Argonaute (AGO) proteins to characterize strengths and s

109 MicroRNAs (miRNAs) associate with Argonaute (AGO) proteins to direct widespread posttransc

110 MicroRNAs are loaded into Argonaute (AGO) proteins to form the RNA-induced silenci

111 In plants, small RNAs are loaded into ARGONAUTE (AGO) proteins to fulfill their regulatory fun

112 MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to repress target messenger RNA

113 e small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcr

114 (miRNAs) act as sequence-specific guides for Argonaute (AGO) proteins, which mediate posttranscriptio

115 e through the action of DICER-like (DCL) and ARGONAUTE (AGO) proteins.

116 Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis o

117 e proteomics, and RNA immunoprecipitation of Argonaute (Ago), a component of the RNA-induced silencin

118 We identified homologs of DICER-LIKE (DCL), ARGONAUTE (AGO), and other genes involved in small RNA p

119 MicroRNAs (miRNAs) associate with Argonaute (Ago), GW182, and FXR1 proteins to form RNA-in

120 ate gene expression through association with Argonaute (Ago), which also protects miRNAs from degrada

121 NA)-guided transcriptional gene silencing by Argonaute (Ago)-containing complexes is fundamental to g

122 derstood, but recent data have identified an Argonaute (AGO)-dependent siRNA pathway.

123 through small interfering (si) RNA-directed, ARGONAUTE (AGO)-mediated cleavage of homologous transcri

124 distant from fungi, employ the host plant's Argonaute (AGO)/RNA-induced silencing complex for virule

125 Argonaute crosslinking immunoprecipitation (Argonaute [Ago]-CLIP) sequencing in miR-122 knockout and

126 are enriched for 5' nucleotide bias against Argonaute-(AGO)-loading, but also additional 3' and cent

127 ), Tas3 (protein with unknown function), and Argonaute (Ago1), plays an important role in RNAi-mediat

128 s further revealed no significant changes of argonaute (AGO1, AGO2) and endoribonuclease dicer (DICER

129 on between circulating miRNAs and the miRISC Argonaute AGO2.

130 Using purified miRNA-Argonaute (AGO2) complexes, synthetic target RNAs, and a

131 that IGF2BP2 is an accessory protein of the argonaute (AGO2)-miR-33a/b-RISC complex, as it directly

132 Argonautes (AGOs) associate with noncoding RNAs to regul

133 However, the identity and role(s) of Argonautes (AGOs) involved in herbivory remain unknown.

134 imited to near perfect, straight duplexes in Argonautes (Agos).

135 n a Caenorhabditis elegans microRNA-specific Argonaute ALG-1 (Argonaute-like gene) that are antimorph

136 ng the rules for nucleic acid hybridization, Argonautes allow oligonucleotides to serve as specificit

137 l conserved roles for RNAi proteins, such as Argonaute and Dicer, in chromosome function.

138 We also performed knockdown of argonautes and rnaseh separately along with f7.

139 tribution of distinct miRNAs between the two Argonautes and the ability of one of them to load additi

140 RNAi genes including those coding for Dicer, Argonaute, and double-stranded RNA-binding proteins (dsR

141 ate-binding pocket in the Dicer-2 PAZ (Piwi, Argonaute, and Zwille/Pinhead) domain is crucial for the

142 Argonautes are also present in many bacterial and archae

143 l similarities between human and prokaryotic Argonautes are consistent with shared mechanistic proper

144 domains of life and suggests that eukaryotic Argonautes are derived from DNA-guided DNA-interfering h

145 NA hybridization-a small RNA or DNA bound to Argonaute as a guide no longer follows the well-establis

146 d suggest that stress-induced alterations in Argonaute-associated sRNAs can target the deployment of

147 pression was mediated by a redistribution in Argonaute association, from let-7 to non-let-7 microRNA

148 ion systems (R-M), abortive infection (Abi), Argonaute-based interference, as well as clustered regul

149 h hAgo via three of the GW/WG repeats in its Argonaute-binding domain: motif-1, motif-2, and the hook

150 Argonaute bound ~1400 erythroblast mRNAs in a miR-144/45

151 In cell lines, Argonaute-bound microRNAs exist mainly in high molecular

152 used CLEAR (Covalent Ligation of Endogenous Argonaute-bound RNAs)-CLIP (Cross-Linking and Immuno-Pre

153 ermed CLEAR (covalent ligation of endogenous Argonaute-bound RNAs)-CLIP, which enriches miRNAs ligate

154 tential role of the NTD in recruiting Nbr to Argonaute-bound small RNA substrates.

155 detected endogenous let-7 miRNA-induced and Argonaute-catalysed endonucleolytic cleavage on target m

156 long tRF-3s and tRF-5s that could enter into Argonaute complexes are not induced by ANG overexpressio

157 y canonical seed-pairing site to recruit the Argonaute complexes.

158 the function might require interaction with Argonaute components of the silencing machinery, as was

159 ructure and silence transcription by guiding Argonaute-containing complexes to complementary nascent

160 originating from long noncoding RNAs, guide Argonaute-containing effector complexes to complementary

161 To this end, we performed Argonaute crosslinking immunoprecipitation (Argonaute [A

162 rhabditis elegans has revealed the essential Argonaute CSR-1 to play key nuclear roles in modulating

163 istinct pathway that presumably involves the Argonaute CSR-1.

164 uencing the efficiency of siRNA loading into Argonaute, degradation of cleaved passenger strand and d

165 s, including regulating protein translation, Argonaute-dependent gene silencing, and more.

166 Argonautes distinguish substrates from targets with simi

167 We validate our approach using Argonaute eCLIP-seq and ribosome profiling, demonstratin

168 hopping products are selectively loaded onto Argonaute, enabling DNA-guided defense against foreign D

169 nd structure-based alignments suggested that Argonautes encoded within CRISPR-cas [clustered regularl

170 umour suppressor activity for the Drosophila Argonaute family RNA-binding protein AGO1, a component o

171 encoding Argonaute5 (AGO5), a member of the Argonaute family.

172 microRNA* strands present in the ALG-1(anti) Argonaute far in excess of the corresponding mature micr

173 Here, we report that Argonaute from the archaeal organism Methanocaldococcus

174 ic Acids of clinical interest Via DNA-Guided Argonaute from Thermus thermophilus (TtAgo).

175 Biochemical assays have demonstrated that Argonaute functions by modulating the binding properties

176 A pathway genes Dicer, Dgcr8, and the entire Argonaute gene family (Ago1, 2, 3, and 4).

177 The Piwi-like genes belong to the Argonaute gene family and are conserved in plants, anima

178 with archaeal proviruses and genes linked to Argonaute genes in halobacteria.

179 Within Argonaute, guide strands have stabilities that vary by 1

180 target binding and cleavage mediated by the Argonaute:guide complex, RISC.

181 cing, the physical interaction between human Argonaute (hAgo) and GW182 (hGW182) is essential for fac

182 the knockdown of f7 occurs when knockdown of argonautes happens and not when rnaseh knockdown was per

183 The RNA cleavage ("Slicer") activity of Argonaute has been implicated in both sRNA maturation an

184 A-binding sites for cZNF292 were detected in Argonaute high-throughput sequencing of RNA isolated by

185 Additionally, the Piwi Argonaute homolog PRG-1 and its downstream molecular com

186 e PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that maintains trans-generational silen

187 ID-1, a primary Argonaute RDE-1, a secondary Argonaute HRDE-1, and an RNase D homolog MUT-7, maintena

188 into the nuclear RNAi pathway involving the Argonaute HRDE-1, concurrent with a reduction in the exp

189 hway to produce siRNAs that bind the nuclear Argonaute HRDE-1, resulting in dramatic defects in germ

190 arius physically interacts with the germline Argonaute HRDE-1.

191 Both set-32 and the germline nuclear RNAi Argonaute, hrde-1, are required for nuclear RNAi-induced

192 were generated by cross-linking followed by Argonaute immunoprecipitation and sequencing (Ago CLIP-s

193 Using genetic manipulations, Argonaute-immunoprecipitations and high-throughput seque

194 tified and characterized a family of nuclear Argonaute-interacting proteins (ENRIs) that control the

195 ovide a near-comprehensive analysis of miRNA-Argonaute interactions in C. elegans and reveal a new ro

196 lect different plant Dicer-like proteins and Argonautes involved in vsiRNA biogenesis.

197 These findings demonstrate that the role of Argonautes is conserved through the bacterial and archae

198 s elegans microRNA-specific Argonaute ALG-1 (Argonaute-like gene) that are antimorphic [alg-1(anti)].

199 combined results reveal the mechanisms that Argonaute likely uses to efficiently identify miRNA targ

200 ally assess their significance, we sequenced Argonaute-loaded microRNAs to define functionally engage

201 r TUTases at its proximity without involving Argonaute-mediated RNA cleavage.

202 ement of relative binding affinities between Argonaute-miRNA complexes and all sequences <=12 nucleot

203 the CRISPR-associated Marinitoga piezophila Argonaute (MpAgo) protein cleaves single-stranded target

204 city, the archaeal PfAgo resembles bacterial Argonautes much more than eukaryotic Argonautes.

205 ENRI-1/2 prevent misloading of the nuclear Argonaute NRDE-3 with small RNAs that normally effect ma

206 is compaction requires the small RNA-binding Argonaute NRDE-3, the pre-mRNA associated factor NRDE-2,

207 the viral sensor DRH-1/RIG-I and the nuclear Argonaute NRDE-3.

208 Here we analyse the activity of a bacterial Argonaute nuclease from Clostridium butyricum (CbAgo) in

209 The Argonaute of the archaeon Pyrococcus furiosus (PfAgo) be

210 Recently, Argonautes of the bacteria Rhodobacter sphaeroides and T

211 Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to prima

212 Functions of prokaryotic Argonautes (pAgo) have long remained elusive.

213 Prokaryotic Argonautes (pAgos) are much more diverse than their euka

214 ic gap, we characterize the functions of two Argonaute paralogs in the sea anemone Nematostella vecte

215 , the tuning action of a small RNA-catalytic Argonaute pathway generates oocytes capable of supportin

216 Here, we describe a small RNA-Argonaute pathway that ensures early embryonic divisions

217 s both Dicer-dependent and Dicer-independent Argonaute pathways and provide insight into how cells an

218 led to the emergence of small RNA-catalytic Argonaute pathways in the female germline as a post-tran

219 ntersecting forebrain miR profiles that were Argonaute precipitated, synaptic vesicle target enriched

220 Silencing requires the PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that ma

221 NA (piRNA) cluster; we observe that the Piwi Argonaute PRG-1 is involved in the regulation of nictati

222 iota-dependent S-nitrosylation of C. elegans Argonaute protein (ALG-1)-at a site conserved and S-nitr

223 Here, we describe a specific Argonaute protein (exWAGO) that is secreted in extracell

224 the host environment and identifies a novel Argonaute protein as the mediator of this.

225 e sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Dro

226 ponent of P granules, the endo-siRNA-binding Argonaute protein CSR-1, has recently been ascribed with

227 CSR-1 is a germline-expressed C. elegans Argonaute protein essential for viability.

228 However, both Dicer and the Argonaute protein family have expanded roles in gene reg

229 Members of the conserved Argonaute protein family use small RNA guides to locate

230 died, DNA-guided DNA endonuclease, TtAgo, an Argonaute protein from the Eubacterium Thermus thermophi

231 hod for investigating an early step in RNAi, Argonaute protein loading with small RNAs, which is enab

232 his manuscript describes the functions of an Argonaute protein named AGO17 in rice.

233 PIWIL3 is an Argonaute protein of the PIWI subfamily that is mainly e

234 f guide-independent cleavage activity for an Argonaute protein potentially serving as a guide biogene

235 RNAe is promoted by the Piwi Argonaute protein PRG-1 and associated Piwi-interacting

236 phate of the guide strand of an siRNA by the Argonaute protein.

237 As (miRNAs) and Y RNAs as well as a nematode Argonaute protein.

238 Mammalian Argonaute proteins (AGO1-4), in combination with microRN

239 MicroRNAs (miRNAs) associated with Argonaute proteins (AGOs) regulate gene expression in ma

240 chanism dependent on the slicing activity of Argonaute proteins (AGOs)(8,9).

241 Prokaryotic Argonaute proteins acquire guide strands derived from in

242 We further uncover a requirement for Argonaute proteins and Dicer, factors involved in small

243 l physiology and disease by associating with Argonaute proteins and downregulating partially compleme

244 eins, though they seem to be associated with ARGONAUTE proteins and have few potential targets.

245 lant tRFs are sorted into different types of ARGONAUTE proteins and that they have potential target c

246 "seed" mechanism reminiscent of that used by Argonaute proteins during RNA interference in eukaryotes

247 Here, we review current understanding of Argonaute proteins from a structural prospective and dis

248 arts et al. (2017) discover that prokaryotic Argonaute proteins generate their own DNA guide strands

249 In many eukaryotes, siRNAs bound to Argonaute proteins guide chromatin-modifying enzymes to

250 The application of CLIP to Argonaute proteins has expanded the utility of this appr

251 Despite the relevance of Argonaute proteins in RNA silencing, little is known abo

252 miRs were readily co-immunoprecipitated with Argonaute proteins in vivo and were active in luciferase

253 Eukaryotic Argonaute proteins induce gene silencing by small RNA-gu

254 Argonaute proteins loaded with microRNAs (miRNAs) or sma

255 Argonaute proteins mediate gene regulation by small RNAs

256 Argonaute proteins play a central role in mediating post

257 Argonaute proteins repress gene expression and defend ag

258 We find that both eukaryotic and prokaryotic Argonaute proteins reshape the fundamental properties of

259 PIWI clade Argonaute proteins silence transposon expression in anim

260 ely 23 to 30 nucleotides bound to PIWI clade Argonaute proteins silence transposons in a manner that

261 RNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation

262 ptional regulation of human genes by guiding Argonaute proteins to complementary sites in messenger R

263 MicroRNAs (miRNAs) act within Argonaute proteins to guide repression of messenger RNA

264 base-pair to messenger RNA targets and guide Argonaute proteins to mediate their silencing.

265 microRNAs (miRNAs) guide Argonaute proteins to mRNAs targeted for repression.

266 ense against transposable elements and guide Argonaute proteins to nascent RNA transcripts to induce

267 icroRNAs repress mRNA translation by guiding Argonaute proteins to partially complementary binding si

268 miRNAs are small RNAs that guide Argonaute proteins to specific target mRNAs to repress t

269 29 nucleotide (nt) small RNAs complexed with argonaute proteins to suppress parasitic mobile sequence

270 Argonaute proteins use microRNAs (miRNAs) to identify mR

271 ike eukaryotic proteins, several prokaryotic Argonaute proteins use small DNA guides to cleave DNA, a

272 omoting loading of derivative 5p miRNAs into Argonaute proteins via a Dicer-coupled 5' monophosphate-

273 stems, including innate immunity centered on Argonaute proteins, bacteriophage exclusion, and new typ

274 In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defen

275 progress has been made toward understanding Argonaute proteins, small RNAs, and their roles in eukar

276 RdRP recruitment and mRNA silencing require Argonaute proteins, which are generally thought to degra

277 21/24-nt siRNAs as well as their associated Argonaute proteins.

278 oRNAs are tethered to their target mRNAs by "Argonaute" proteins.

279 ithin the germline requires SID-1, a primary Argonaute RDE-1, a secondary Argonaute HRDE-1, and an RN

280 egans, the enzymatic activity of the primary Argonaute, RDE-1, is not required for silencing activity

281 ) and 'activating' siRNAs bound by the CSR-1 Argonaute require the DRH-3 helicase, an RdRP component.

282 ison of EndoV with its homologs RNase H1 and Argonaute reveals the principles by which these enzymes

283 use partial base-pairing characteristics of Argonaute RNPs to bind mRNAs randomly in Drosophila, act

284 Argonaute sequencing generated 1.44 billion small RNA re

285 additional evidence that fission yeast siRNA-Argonaute silencing complexes are recruited to target lo

286 e DNA sequence of target loci to which siRNA-Argonaute silencing complexes are recruited.

287 Typically, Argonaute slices and releases the passenger strand of du

288 gether, our data suggest the evolution of an Argonaute subclass with noncanonical specificity for a 5

289 ich have an unusual Dicer and a conventional Argonaute that are both required for gene silencing.

290 ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role i

291 ntary mRNA targets while in association with Argonaute, the effector protein of the miRNA-mediated si

292 We use Pyrococcus furiosus Argonaute to punch files into the PCR products of Escher

293 However, the mechanisms used by Argonaute to reshape the binding properties of its small

294 cterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA pl

295 tic structural study of Thermus thermophilus Argonaute (TtAgo) ternary complexes containing single-ba

296 Argonautes use the nucleotide sequences in miRNAs as gui

297 th 'silencing' siRNAs bound by Worm-specific Argonautes (WAGO) and 'activating' siRNAs bound by the C

298 erference (RNAi) pathway proteins, including Argonaute, were also present in amebic EVs.

299 These duplexes are rapidly loaded into Argonaute, with <30 min typically required for duplex lo

300 Dicer is phosphorylated in the platform-Piwi/Argonaute/Zwille-connector helix cassette upon induction