ライフサイエンスコーパス: assay

1 and respiratory syncytial virus (RSV) (FFABR assay).

2 uct (1.4% relative to BC-1 upon fluorescence assay).

3 different cells in an antagonistic migration assay.

4 and GNRR-6 in a cellular receptor activation assay.

5 imaging assay and a [(3)H]ketanserin binding assay.

6 in Nicaragua using a Multi-Color FluoroSpot assay.

7 for Industry to evaluate the quality of the assay.

8 to GCAUG in a trichromatic splicing reporter assay.

9 scription factor activity in a reporter cell assay.

10 dent from the originating source, project or assay.

11 ualizing and quantitative measurement of the assay.

12 splantation is the single antigen bead (SAB) assay.

13 re determined using a multiplex protein bead assay.

14 deoxyuridine triphosphate nick end labeling assay.

15 IgG4, and donor-specific antibody by Luminex assay.

16 ose demonstrated by the duplex real-time PCR assay.

17 d perichondrium, for a novel tissue identity assay.

18 targets that were detected by the ePlex RPP assay.

19 in reaction, and enzyme-linked immunosorbent assay.

20 ly established NanoBRET-based ligand binding assay.

21 is demonstrated using standard cytotoxicity assays.

22 ly)] in serum using electrochemiluminescence assays.

23 ulture the virus in vitro for neutralization assays.

24 cytometry, immunofluorescence, and organoid assays.

25 re quantified using electrochemiluminescence assays.

26 , and short hairpin RNA-based gene-silencing assays.

27 22,236 mosquitoes tested from 96 independent assays.

28 was carried out using both live/dead and MTT assays.

29 ty based on results from DPPH, ABTS and ORAC assays.

30 ecalSwab specimen was loaded onto the BD Max assays.

31 tionally, conductive to sarcomere shortening assays.

32 riments, such as genome editing and reporter assays.

33 indistinguishable phenotypes across multiple assays.

34 of useful meat industrial multiplex-proteins assays.

35 immunoprecipitation and luciferase reporter assays.

36 ified by the conventional glycogen detection assays.

37 in both cell-based and in vitro translation assays.

38 n exhibited high enzyme activity in in vitro assays.

39 those for three different P. jirovecii qPCR assays.

40 way to simple and inexpensive point-of-care assays.

41 lating effects observed in tethered function assays.

42 ding domain total antibody chemiluminescence assay (100% sensitivity, 99.8% specificity).

43 sk neuroblastoma, we integrated the C-circle assay [a marker for alternative lengthening of telomeres

44 eprae, were validated as clinical diagnostic assays according to Clinical Laboratory Improvement Amen

45 The assay accuracy was validated by titration of 10-80% mixt

46 of the genome, transcriptome, and functional assays advance general understanding of the Antarctic gr

47 The assay allowed a rapid and high-sensitive C. acutatum det

48 This assay allows distinction of two separate binding sites f

49 Subsequent in vitro wound-healing assays also confirmed that M2 and M13 accelerate the wou

50 pressing HEK293 cells using a Ca(2+) imaging assay and a [(3)H]ketanserin binding assay.

51 Pain was assessed by von Frey assay and dorsal root ganglia (DRG) expression of Calca

52 ain (NfL), using enzyme-linked immunosorbent assay and electrochemiluminescence.

53 l transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3.

54 2 with an IC(50) = 0.031 muM in an enzymatic assay and with an IC(50) = 2.6 muM in human pancreas cel

55 Its validity was demonstrated in cellular assays and a murine colitis model expressing hPXR by a s

56 g new therapeutics requires reliable kinetic assays and an analysis framework to extract kinetic phar

57 Propidium iodide/annexin assays and caspase 3, caspase 7, and PARP-1 analyses sho

58 , we performed ex vivo whole-blood infection assays and confrontation assays with primary human NK ce

59 Transient transformation assays and expression analyses in mutants reveal that, i

60 mitochondrial respirometry, enzyme activity assays and gene expression analyses.

61 n vitro high-throughput EpiSELEX-seq binding assays and in vivo methylated TFBSs from the MeDReaders

62 quence, both in in vitro biochemical binding assays and in vivo studies of differentially methylated

63 e and synergy with Cappuccino in bulk pyrene assays and on beads.

64 ed by inhibition enzyme-linked immunosorbent assay) and dengue severity, categorized using 3 definiti

65 to the promoter region of VE-cadherin (chip assay) and is induced by VEGF in DPSCs.

66 The RLPM assay, and a previously developed RLEP quantitative poly

67 nd Matrigel invasion assay, sphere formation assay, and in vivo mice tumor model were performed to ev

68 2 using luminescence- and fluorescence-based assays, and by analyzing ribosome-profiling and mass spe

69 tum coherence spectra, kinetics, biochemical assays, and MS analyses, we show that the conserved nucl

70 e experiments from the literature, simulated assays, and primary MPRA data.

71 , liposomal nanoallergen display, bead-based assays, and protein chimeras have been used in epitope d

72 (primary analysis) and anti-nucleocapsid IgG assays, and staff members were followed for up to 31 wee

73 ves ssDNA, dsDNA and RNA targets in a single assay; and operates at elevated temperatures, providing

74 GNP-HCIm cytotoxicity was evaluated by MTT assay, apoptosis/necrosis and phosphorylated-cAbl (cAbl-

75 Robust serological assays are essential for long-term control of the COVID-

76 en that the commercially available serologic assays are highly variable, differing in their format, t

77 using a comprehensive sequence of behavioral assays, as well as measures of health and development hi

78 ement (PPA) of 98.7%, followed by the Aptima assay at 94.7%, compared to the consensus result.

79 Furthermore, chromatin assays (ATAC-seq) demonstrate that Opa, like Zld, influe

80 CSF antibodies or SSM5, we used a functional assay based on cytosolic Ca(2+) imaging.

81 g laser (VCSEL), coupled with a fast protein assay based on protein-induced fluorescence enhancement

82 ty to PIPs we have developed a new cell free assay based on the ability of the receptor to prevent al

83 Bead-based epitope assay (BBEA) was used to quantitate the levels of epitop

84 y of the BD MAX multidrug-resistant (MDR)-TB assay (BD MAX) in South Africa, Uganda, India, and Peru.

85 conjugates to establish a direct competitive assay between the m6A target and a biotinylated RNA olig

86 ds, indicating that the cannabinoid reporter assay can be used for an estimation of drug concentratio

87 it is very stable and most of BS conversion assays can achieve> 99.5% efficiency.

88 e of the cobas Trichomonas vaginalis (TV)/MG assay (cobas) for the detection of M. genitalium, using

89 Heterogeneous assays commonly utilize magnetic beads as a solid phase.

90 informatics analysis and luciferase reporter assay confirmed that miR-466o-3p directly targeted WT1 m

91 In vitro transport assays confirmed that some of these compounds were in vi

92 te that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis prov

93 oughput phage immunoprecipitation sequencing assay covering the complete reference protein sequences

94 ed with a lateral flow immunochromatographic assay (CTV-RT-RPA-LFICA).

95 adsorption isotherms and guest displacement assays demonstrate that the adsorbed cages retain the ab

96 and E. dispar), our tetraplex real-time PCR assay demonstrated levels of sensitivity and specificity

97 Reporter assays demonstrated that these LTR12C elements drive gen

98 The QIAstat-Dx RP assay detected 312 (92%) of the 338 respiratory targets

99 been guided by biomarkers or clinical-grade assays developed to predict patient response and, ultima

100 The assay development and qualification data for this origin

101 ill be useful for antibody screening, custom assay development, biomarker detection, and protein prof

102 ASV RDT to available quantitative PCR (qPCR) assays during the 2018 LF outbreak in Nigeria.

103 hese antigens by enzyme-linked immunosorbent assay (ELISA) using sera from naive rabbits and rabbits

104 veloped a simple enzyme-linked immunosorbent assay (ELISA)-based assay that uses the temperature-depe

105 testing the ability of the platform for drug assays employing cyclic drug exposure regimens.

106 only used is the "Endothelial Tube Formation Assay" (ETFA).

107 The SSH assay exhibited a linearity of 7 logs with a lower limit

108 o B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in pat

109 Here, we performed in vitro integration assays, finding that for G1 and G2, the insertion site i

110 Mean differences favored the MN assay for A/H1N1 and B strains in study 1, whereas the t

111 suggest that CaAR can be used as a sensitive assay for INF2 function and for robust evaluation of dis

112 assays for respiratory viruses, including an assay for influenza A (FluA) virus, influenza B (FluB) v

113 RLEP quantitative polymerase chain reaction assay for M. leprae, were validated as clinical diagnost

114 However, a higher-order multiplexing dPCR assay for measuring SMN1 and SMN2 copy numbers in spinal

115 ently developed an organotypic slice culture assay for sensitive detection of scrapie prions using ul

116 loped a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B.

117 Finally, we modified a proximity ligation assay for tissue sections and found that Ascl1-Gsx2 inte

118 constructed a continuous spectrophotometric assay for transpeptidation of native or near native pept

119 rge-scale comparison of commercial molecular assays for detection of M. pneumoniae in the United Stat

120 tive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses, including an assay for i

121 ostic manufacturers have developed molecular assays for SARS-CoV-2 under the Food and Drug Administra

122 mance comparable to those of other molecular assays for the detection of B. pertussis and B. parapert

123 re can be readily applied to dual-parametric assays for various targets.

124 y serve as a parameter for future diagnostic assays for women with breast implants to distinguish ser

125 ent and qualification data for this original assay format are presented along with its application to

126 orrelation found that the Fusion and BioFire assays had a positive percent agreement (PPA) of 98.7%,

127 It was found that the dual-analyte assays had identical analytical characteristics with the

128 apid growth in complexity of data from these assays has outpaced our ability to accurately infer phyl

129 Indicator displacement assays (IDAs) offer a unique and innovative approach to

130 compared with those from immunofluorescence assays (IFA).

131 or sensitivity of immunofluorescent-antibody assays (IFAs), a reliable serodiagnostic test for canine

132 was not obtained in at least 1 confirmatory assay in 12.0% (10/83) and in an HIV rapid test in 31.3%

133 A three-dimensional organoid assay in colonic crypts isolated from CR-infected mice r

134 apenemase genes were detected by the Carba-R assay in Klebsiella pneumoniae (n = 236), Escherichia co

135 the accuracy and reproducibility of the qSVS assay in quantifying authentic HIV minority variants, an

136 the gold standard Limulus Amoebocyte lysate assay in real bacteria culture containing naturally occu

137 SP and IL-1beta levels were assayed in the culture medium by ELISA.

138 f fission yeast in 59,350 individual fitness assays in 70 conditions, revealing that colony size and

139 analysis of patient samples, and phenotypic assays in personalized cancer avatars.

140 h-throughput in vitro transporter inhibition assays in the early ADME profiling space in drug discove

141 hnologies, representing more than 70% of the assays in the US Tox21 research consortium.

142 nalyses of HK phosphorylation in biochemical assays in vitro suggest negative cooperativity, whereby

143 cription by T7 RNAP were confirmed with this assay, including the importance of enzyme and substrate

144 Limited proteolysis assays indicate that acetylation of Pif1 induces a confo

145 complementation studies and enzyme kinetics assays indicate that B. fragilis UroS is functionally di

146 Ensemble in vitro transcription assays indicate that PQS in the non-template increases m

147 The Chrome Azurol S assay indicated that these metabolites bind ferric iron,

148 Resazurin and crystal violet assays indicated that 8a decreases triple-negative breas

149 In vitro assays indicated that Sl-LIP8 can cleave 18:2 and 18:3 a

150 nstrate that the RNA mutation-based APOBEC3A assay is applicable to clinical samples from cancer pati

151 The assay is capable of simultaneously quantifying multiple

152 One of the key steps in the assay is converting unmethylated cytosines into thymines

153 , which was a full agonist in Ca(2+)-release assays; its potency and binding affinity for Ins(1,4,5)P

154 Functional assays (killing, phagocytosis, transmigration, and respi

155 However, most widely used clinical assays lack patient-matched control DNA and additional a

156 ise ratio in a fluorescent Dulbecco's plaque assay, leading to the construction of a multireporter pl

157 The Aspergillus Galactomannan Lateral Flow Assay (LFA) is a rapid test for the diagnosis of invasiv

158 ated a proof of concept for the lateral flow assay (LFA).

159 low cost and quick operation of lateral flow assays (LFA) have made them some of the most common poin

160 In live cell-based assays, LGI1 epitope recognition was examined with patie

161 compare Veritor with the Lyra SARS-CoV-2 PCR assay (Lyra).

162 ular fluid using enzyme-linked immunosorbent assay method and assessed with clinical parameters using

163 n this study, a high-throughput fluorescence assay method for determination of xanthine oxidase (XO)

164 , we assayed two massively parallel reporter assay (MPRA) libraries composed of binding sites for SOX

165 In the massively parallel reporter assay (MPRA), short barcodes are counted by sequencing D

166 an integrative strategy including enzymatic assays, mutant analysis, metabolic engineering, isotope

167 Here, we report the reagentless impedimetric assay of two internal exosome markers (alpha-synuclein a

168 e developed a purification strategy enabling assaying of individual C-subtypes and impurities for oto

169 primarily has been tested indirectly through assays of channel function.

170 lysis of cSCC tissue sections and functional assays of cSCC cells in culture showed that LINC00346 ex

171 ampered by inappropriately tailored in vitro assays of drug response.

172 top associated miRNAs by luciferase reporter assays of glucocorticoid-mediated transrepression and pr

173 Microarray and SPR assays of structurally defined HS oligosaccharides show

174 The whole blood ELISpot assay offers a significant advance in the ability to imm

175 These assays often use elongation inhibitors to purportedly in

176 e microscopy and Lactate dehydrogenase (LDH) assay on the supernatant.

177 We also performed genetic assays on 5 years of field samples that showed persisten

178 e over monolithic sensors because of simpler assay optimization and improved selectivity.

179 itu hybridization, and a transgenic reporter assay, our results demonstrate a requirement for dnmt1 a

180 Assay performance was evaluated in four clinic-based pro

181 However, no such asymmetry was observed in assays performed in the presence of Munc18-1, Munc13-1,

182 higher in a membrane environment compared to assays performed with water-soluble quinone analogues, d

183 Using a multiplex system, we assayed plasma biomarkers related to tubular injury, inf

184 porcine and fish gelatin species in a single assay platform.

185 ccount for the sensitivity of the laboratory assays, prospective community screenings, and healthcare

186 cfDNA, measured by a rapid simple assay, proved a valuable early marker of severity in ABP

187 We review methods for assaying putatively functional genetic variants and regi

188 However, most current assays report cell population averages and are mostly ti

189 To date, however, antioxidant assays require costly instrumentations, laboratory setup

190 In vivo mouse reconstitution assays resealed that only CD69(High) LSCs were capable o

191 Notably, short-term acute heat stress assays resolved per-colony (genotype) differences that m

192 tt IgG, Roche total antibody, and Abbott IgM assays, respectively, with sampling times 0 to 56 days p

193 ted by immunoblotting, ELISA, and cell death assays, respectively.

194 ePlex RPP, NxTAG RPP, and Mycoplasma Direct assays, respectively.

195 mization of this chemical cleavage acDrug PK assay, resulting in robust accuracy and precision (+/-20

196 RNA immunoprecipitation (RIP) and mRNA-decay assays reveal that QKI-7 binds and promotes mRNA degrada

197 Quantitative biochemical assays reveal that RALF4 binds LLGs and LRX cell-wall mo

198 A time-kill assay revealed that auranofin exhibited rapid bactericid

199 And finally, in vitro capillary sprouting assays revealed that inhibition of IL-6 or STAT3 signali

200 Chemotaxis assays revealed that receptor-mediated adaptation to ind

201 scopy, X-ray diffraction, and gelatinization assays revealed that ultrasound treatment induced change

202 A click chemistry-based flux assay reveals that necrocytosis provides not only amino

203 ly conserved genomic regions make pan-family assays robust and resilient to mutation.

204 Finally, we provide an example of the assay's ability to identify and characterize novel compo

205 We used a combination of multiplex SNaPshot assays, Sanger sequencing, mutation-specific PCR, or dro

206 confidence intervals (CI) were adjusted for assay sensitivity and specificity.

207 TM and RT-PCR in a community setting, rapid assay sensitivity was 100%/98.5%/89% using RT-PCR Ct thr

208 e signal), linearity (range 0.05-5.00 mg per assay), sensitivity (limit of detection and quantificati

209 ng time-dependent inhibition that simplifies assay setup and allows comparison of inhibition models i

210 Cytotoxicity assays show good correlation at the outer limits of thio

211 Behavioral assays show that butterflies use wings to sense visible

212 Our reporter assay showed that volatile anesthetics isoflurane and se

213 ion titres determined using LASV pseudovirus assays showed significant correlation with titres determ

214 Cell fate assays showed that multicolor flow cytometry and transcr

215 urinary volatile organic compounds and ELISA assays showed that the loading-conditioned urine reduced

216 Both in vitro and in vivo assays showed that TubZIP28 bound to the promoter of cyt

217 The assay shows excellent replicate consistency with 8% intr

218 as measured in the standard membrane feeding assay (SMFA).

219 To assay spatial working memory, all animals performed a re

220 Included in the Tox21 portfolio are assays specifically designed to measure interference in

221 Wound healing and Matrigel invasion assay, sphere formation assay, and in vivo mice tumor mo

222 Conjugate PK assay strategies for these ADCs involve cleavage with ca

223 Electrophoretic mobility shift assay suggested that the G allele interacted with CCAAT/

224 uced bacterial killing in opsonophagocytosis assays, suggesting that HN13 LTA is an attractive target

225 In liquid assay system, both GO and MGO inhibited the target bacte

226 In agarised assay system, GO and MGO exhibited antimicrobial activit

227 antly lower than those estimated in agarised assay system.

228 ei imaging, and the telomere shortest length assay (TeSLA), we show that chromosome mis-segregation d

229 roughput microscopy-based retrotransposition assay that identified the double-stranded break (DSB) re

230 able to detect and quantitate cGAMP with an assay that is high-throughput, sensitive, and precise.

231 etected from NP samples via a direct RT-qPCR assay that omits the RNA extraction step altogether.

232 in tumor samples from patients, employing an assay that readily detects these intercellular protein-p

233 yme-linked immunosorbent assay (ELISA)-based assay that uses the temperature-dependent loss of confor

234 Using a FRET-based high-throughput screening assay that we previously reported, we identified small-m

235 primarily to cost, the total number of such assays that can be performed is limited.

236 rts, double-blinded index test and detection assays that do not require RNA extraction.

237 vily dependent upon conventional biochemical assays that retain the challenges of being time-consumin

238 atients' primary fibroblasts and biochemical assays, that these mutations either impair UGDH stabilit

239 Unlike other imaging-based endocytosis assays, the ppH protocol detects EVs without a priori hy

240 termine the optimum anti-Xa heparin activity assay therapeutic range during extracorporeal membrane o

241 transitioning from a conventional troponin I assay to a high-sensitivity assay with sex-specific thre

242 Here, we developed a novel in vitro assay to characterize the length and position of individ

243 ne or in combination with the anti-spike IgG assay to determine baseline status.

244 ated by indirect immunofluorescence antibody assay to identify immunoglobulin G (IgG) antibodies reac

245 d as reporters in a competitive displacement assay to identify novel hit matter.

246 used a large-scale enhanced yeast one-hybrid assay to identify potential regulators of vascular ident

247 Use of the LIPS assay to identify putative "diagnostic" proteins would b

248 We used an enzyme-linked immunosorbent assay to measure levels of spontaneous release of molecu

249 rein described a convenient and quantitative assay to monitor dynamic FAO activities of mammalian cel

250 e, we describe the development and use of an assay to monitor the incidence and treatment of CMV vire

251 Here we used a modified Ramsay assay to quantify the impact of Malpighamoeba infection

252 and a spatially resolved promoter activation assay to study signal exchange in and out of the 200 nm

253 Here, we employ a fluorescence-quenching assay to study the binding of ssrA-tagged substrates to

254 rved yield of full-length protein in the FPA assay to the force exerted by the folding protein in pic

255 Yet, many current in vitro chemical assays to characterize antioxidant potential do not trul

256 spectrometry-based proteomic and phenotypic assays to demonstrate that the expression of active nonc

257 The objective was to estimate performance of assays to detect those infections in pharyngeal and rect

258 cell biological, biochemical, and structural assays to determine that rigosertib, an anti-cancer agen

259 stral protein reconstruction and biophysical assays to elucidate the origins of vertebrate haemoglobi

260 ral data on villin headpiece with functional assays to identify the actin-binding residues in FL vill

261 scribe a strategy utilizing pan-family viral assays to improve early accessibility of large-scale nuc

262 supporting the relevance of ex vivo activity assays to in vivo translation.

263 lations, protein engineering, and functional assays to investigate the role played by conformational

264 able, and readily deployable surrogate viral assays to screen antiviral humoral responses, define cor

265 stral protein reconstruction and biochemical assays to the evolution of steroid hormone receptors, we

266 ompted the need for reliable and noninvasive assays to validate transplant function in clinical bioma

267 ntribute to cis-regulation in mouse ESCs, we assayed two massively parallel reporter assay (MPRA) lib

268 establishes curation of data from screening assays, used routinely in antimalarial drug discovery, a

269 uantitative polymerase chain reaction (qPCR) assay using TaqMan probe was developed to discriminate b

270 lly, the outcome of the cannabinoid reporter assay was compared to the gold standard (LC-MS/MS), show

271 gnal-to-noise ratio, a bioluminescence-based assay was developed against IFN-gamma and provided an op

272 The assay was developed and technically validated.

273 The assay was developed with synthetic DNA templates and val

274 and Bacillus subtilis was observed when the assay was performed on Ab-modified MBs, and E. coli coul

275 were similar when the anti-nucleocapsid IgG assay was used alone or in combination with the anti-spi

276 Using our assay, we have compared synaptotoxic activities in size-

277 Using the in vivo matrigel plug assay, we then found that EC-specific YY1 ablation inhib

278 phase luciferase immune precipitation (LIPS) assays, we characterized IgG, IgM, and IgA antibodies to

279 ere, through the use of in vitro biochemical assays, we demonstrate that the amide backbone of CTA is

280 kinetic analyses, and in vivo cell viability assays, we report that point amino acid substitutions in

281 hythm in U2OS cells, and various biochemical assays, we tested these compounds experimentally and fou

282 tion, cell invasion, and luciferase reporter assays; we measured gene expression, binding activity, c

283 Sensitivity and specificity of the PANDAA assay were determined by a proprietary application desig

284 Individual digital droplet PCR assays were developed for 121 variants (average 5/patien

285 that is detected by conventional biochemical assays which require expensive reagents, is time-consumi

286 y potent inhibitors of DHODH in an enzymatic assay, while also inhibiting cancer cell growth and viab

287 A lipid-mixing assay with confocal imaging reveals that both Syt1 and D

288 ed ubiquitination, we developed a bead-based assay with covalently immobilized but releasable misfold

289 te micropatterns provide a dynamic migration assay with distinct dwell times and relative cell occupa

290 ARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used,

291 ional troponin I assay to a high-sensitivity assay with sex-specific thresholds, in patients with sus

292 Here, we used single-molecule FRET assays with a nanodisc membrane reconstitution system to

293 ug development and clinical practice, robust assays with consistent results are essential.

294 In vitro assays with human enzymes show that PCNA and its loader

295 -Seq, that links massively parallel reporter assays with mass spectrometry to produce a base pair res

296 exposed donors, using interferon-gamma-based assays with peptides spanning SARS-CoV-2 except ORF1.

297 ole-blood infection assays and confrontation assays with primary human NK cells.

298 in A, zinc, and CRP measured using different assays with variable LLOQs.

299 l were detected by sniffers, after migration assay, with high modified frequency (between 50 and 78%)

300 enging results aligned with binding affinity assays, with ACKR2-V41A cells scavenging CCL2 with a low