ライフサイエンスコーパス: autolysis

1 matic effect on murein hydrolase activity or autolysis.

2 XCP2 has a minor but distinct role in micro-autolysis.

3 r through trypsin-mediated cleavages, termed autolysis.

4 f AtlA, and the mutant was hyperresistant to autolysis.

5 els of AtlA protein and led to resistance to autolysis.

6 ytic cleavage site and protects MT1-MMP from autolysis.

7 system that is involved in the regulation of autolysis.

8 growth, and increased cell permeability and autolysis.

9 rected gene expression, cell separation, and autolysis.

10 d in cell separation, biofilm formation, and autolysis.

11 , biofilm formation, genetic competence, and autolysis.

12 s responsible for cell wall breakdown during autolysis.

13 s and is dependent on the intrinsic level of autolysis.

14 um formation and exhibited enhanced cellular autolysis.

15 could be inhibited by the peptide product of autolysis.

16 nsitive to EDTA, a compound known to trigger autolysis.

17 of function of Pep27 or VncRS does not alter autolysis.

18 e virulence genes and more genes involved in autolysis.

19 owed reduced rates of cell wall turnover and autolysis.

20 that, when purified, it apparently underwent autolysis.

21 ctioning mitochondria by processes involving autolysis.

22 e regulators, involved in regulation of cell autolysis.

23 e loss of organelles as a result of cellular autolysis.

24 absence of active MMP-2, suggesting MT1-MMP autolysis.

25 ical content coevolved in proteases to avoid autolysis.

26 refore, it appeared to be an easy target for autolysis.

27 l enzymes that dissolve the cell wall during autolysis.

28 system affects murein hydrolase activity and autolysis.

29 a small but significant decrease in rates of autolysis.

30 oxin pneumolysin through increased bacterial autolysis.

31 analyze the changes in oligomerization upon autolysis.

32 is in the control pathway for triggering of autolysis.

33 umococcal physiology including adherence and autolysis.

34 rus migration through them, concomitant with autolysis.

35 lcium concentrations below that required for autolysis.

36 hibition of cell wall synthesis, and trigger autolysis.

37 el and inner ear cells are very sensitive to autolysis.

38 e cell wall synthesis machinery and triggers autolysis.

39 n1 correlated with a defect in LytA-mediated autolysis.

40 s was much lower than that for the classical autolysis.

41 dly released due to high levels of bacterial autolysis.

42 ted with defects in cell shape and increased autolysis.

43 of extracellular LytA dictates the onset of autolysis.

44 ometry, which triggers the slower process of autolysis.

45 activity, and show increased sensitivity to autolysis.

46 on the autolytic sites of CAPN3, rather than autolysis.

47 ell growth under heat stress, cell division, autolysis, adherence and transformation.

48 essure, which is possibly related to reduced autolysis after exposure to subinhibitory concentrations

49 This strain was unique in showing decreased autolysis after growth in these conditions.

50 ith disintegrating cellular material as mega-autolysis, aided by additional lytic enzymes, destroyed

51 ependently down-regulated sarV (a marker for autolysis), although the alteration in sarV expression d

52 revealed that TiO2 NPs prevent or delay cell autolysis, an important survival and growth-regulating p

53 This reduced ratio increased autolysis and activity of cytoplasmic calpain 1.

54 to be more resistant to Triton X-100-induced autolysis and also to lysis by lysostaphin.

55 ddition, the arlS mutant exhibited increased autolysis and altered peptidoglycan hydrolase activity c

56 toplasmic proteins are released by bacterial autolysis and become adsorbed to the surface of intact b

57 study, the effects of oxidation on calpain I autolysis and calpain-mediated proteolysis were examined

58 However, both autolysis and capsule shedding depend on the cell wall h

59 r postmortem developmental events, including autolysis and chromatin degradation.

60 potential mechanism of Gcp's involvement in autolysis and demonstrated that Gcp may function indepen

61 s membrane integrity, causing bacterial cell autolysis and DNA release.

62 In order to better understand autolysis and in hopes of creating a nonautolytic mutant

63 Strain 126 had a decreased rate of autolysis and increased resistance to lysostaphin degrad

64 ant that exhibited a growth defect, enhanced autolysis and increased sensitivity to Triton X-100 and

65 ion prevented the mature enzyme from limited autolysis and irreversible inactivation.

66 late also had decreased Triton X-100-induced autolysis and killing when incubated in broth media cont

67 reakdown is initially dominated by anaerobic autolysis and later by microbe and insect infiltration,

68 s a protein that induces the host to undergo autolysis and liberate progeny virions.

69 ed type I IFN induction levels and decreased autolysis and lysostaphin sensitivity was found between

70 onomeric forms of MT6-MMP exhibited enhanced autolysis and metalloprotease-dependent degradation.

71 y, (i) an enzyme optimized for resistance to autolysis and oxidation, referred to as the cleavage-res

72 Post-mortem tissue analyses showed autolysis and retention of (210)Po at lethal doses in se

73 depends on the rates of cell wall synthesis, autolysis and the antimicrobial concentration.

74 GelE and SprE--is responsible for regulating autolysis and the release of high-molecular-weight eDNA,

75 ion of 19 potential target genes involved in autolysis and virulence, phenotypes affected by sarA and

76 in with apparent roles in biofilm formation, autolysis, and cell division.

77 differences such as faster growth, increased autolysis, and decreased intracellular hemolytic activit

78 lation of Stk1 function, hemolysin activity, autolysis, and GBS virulence.

79 isms, including specific secretion pathways, autolysis, and membrane vesicle formation.

80 ilm formation, fibronectin-binding capacity, autolysis, and protease and nuclease activities.

81 h, tendency to aggregate in culture, reduced autolysis, and reduced ability to grow under stress, inc

82 of calcium on the enzyme include activation, autolysis, and subunit dissociation.

83 echanism, but rather to bacterial leakage or autolysis, and that the extracellular abundance of these

84 iability following exponential growth due to autolysis, and the necessity for using high starting ino

85 aphylococcus aureus on cell wall metabolism, autolysis, and virulence, mostly in S. aureus laboratory

86 otonmotive-force and preventing the onset of autolysis; and (ii) indirectly, through adsorption of au

87 beta-lactams were dramatically less prone to autolysis as a result of decreased transcription and enz

88 lysin LytA is a virulence factor involved in autolysis as well as in fratricidal- and penicillin-indu

89 asic mechanism of proteolysis and propeptide autolysis, as well as the evolutionary pressures that dr

90 Therefore, prior autolysis at 60 degrees C for 60 min increased the extra

91 e shrimp (Litopenaeus vannamei) subjected to autolysis at 60 degrees C for different times (0, 30, 60

92 For comparison, a classical yeast autolysis at mild temperature (25 degrees C) was perform

93 The PEGylation protected subtilisin-A from autolysis at neutral pH.

94 comparable, and the active enzymes suffered autolysis at similar rates, indicating that neither cata

95 ctive protein is readily inactivated through autolysis at specific internal arginine positions.

96 be determined, several factors influence the autolysis behavior of S. pneumoniae, including the bacte

97 y cellular processes in S. aureus, including autolysis, biofilm formation, capsule synthesis and viru

98 s place in 3 distinct steps: (i) blockage of autolysis by reducing or anaerobic conditions, (ii) rapi

99 molecular-weight excreted molecule, triggers autolysis by self-perturbing the electron transfer react

100 1' position of this cleavage site attenuated autolysis by the enzyme and restored wild-type dimerizat

101 ia and has been conventionally attributed to autolysis by the LytA amidase.

102 o potential mechanisms for the disruption of autolysis by TiO2 NPs in a concentration dependent manne

103 signatures of mRNA exposed up to 37 days of autolysis, can be used to validate the putative identity

104 Here we show that Ply released by autolysis cannot reassociate with intact cells, suggesti

105 heir growth, stress tolerances, respiration, autolysis, cell death, sterigmatocystin production, hyph

106 s involved in different functions, including autolysis, cell division, growth, and pathogenesis.

107 mutant underwent increased stationary-phase autolysis compared to the parental strain.

108 competitive oral biofilm environment, where autolysis could create open spaces for competitors to in

109 The autolysis deficient DeltalytA mutant and its isogenic ch

110 Isogenic wild-type (lyt+) and autolysis-deficient (lyt-) strains of S. aureus were equ

111 g an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aure

112 gether, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains.

113 ometry indicate that the factor Xa underwent autolysis during crystallization and the first EGF-like

114 The role of autolysis during gonococcal infection is not known, but

115 In contrast to classical bacterial autolysis, during capsule shedding, LytA promotes bacter

116 Autolysis, eDNA release, and atlS expression increased s

117 appeared to be more resistant to postmortem autolysis effects.

118 Under conditions that prevent autolysis, embryos within the fertilization envelope can

119 ttle in contact with wine lees because yeast autolysis enriches the wines in colloids and improves th

120 the ability to form disulfide bonds affected autolysis, extracellular DNA release, biofilm formation,

121 cassette, and the mutants were analyzed for autolysis, extracellular DNA release, biofilm formation,

122 s release of the enzyme from bacteria due to autolysis followed by adsorption of the enzyme to the su

123 ing mitochondrial import, but was removed by autolysis following calpain activation.

124 ta-lactone resulted in partial uncoupling of autolysis from differentiation.

125 e also revealed that beta-lactams modify the autolysis function (the natural process of self-exfoliat

126 homogenization (HPH) was tested for inducing autolysis in a commercial strain of Saccharomyces bayanu

127 Ca(2+), Asn-21- and Ile-21-trypsins suffered autolysis in an indistinguishable manner, whereas Thr-21

128 The disruption of autolysis in B. subtilis cultures by TiO2 NPs suggests t

129 eath is a result of loss in cell wall due to autolysis in combination with stinted replenishing.

130 ctam resistance or in the regulation of cell autolysis in E. hirae.

131 It would also induce autolysis in many Gram-positive species, thereby releasi

132 m that has been shown to negatively regulate autolysis in methicillin-sensitive Staphylococcus aureus

133 Lower levels of autolysis in opaque variants, however, was associated wi

134 appears unaffected by conditions that cause autolysis in other eDNA-producing bacteria.

135 ys that control twitching motility, TTSS and autolysis in P. aeruginosa.

136 eB-null mutant exhibits an increased rate of autolysis in response to cell wall-targeting antibiotics

137 by which mgrA and sarA gene products control autolysis in S. aureus.

138 n calpain-mediated proteolysis and calpain I autolysis in situ were examined.

139 usly identified mgrA (rat) as a regulator of autolysis in Staphylococcus aureus.

140 However, amiC mutants still underwent autolysis in stationary phase, indicating that other gon

141 y is presented, as are the possible roles of autolysis in the viral replication cycle.

142 arlRS mutant via a multicopy plasmid induced autolysis in this MRSA strain.

143 of cell wall turnover and detergent-induced autolysis in virtual parallel with the increasing MIC fo

144 gest that agr and sar exert their effects on autolysis, in part, by modulating murein hydrolase expre

145 ed wild-type sensitivity to other classes of autolysis-inducing antibiotics.

146 ed the cell survival of Bacillus subtilis in autolysis-inducing buffer by 0.5 to 5 orders of magnitud

147 An intrinsic substrate of MTG is the dispase autolysis-inducing protein (DAIP).

148 eased enzymes, supporting both mechanisms of autolysis interference.

149 lactams, suggesting that HQNO-dependent cell autolysis is advantageous to the bacterial populations.

150 ng Newman, SH1000, RN6390, and 8325-4, where autolysis is affected by ArlRS.

151 nsitive to protein dilution, confirming that autolysis is intramolecular.

152 ar DNA readily, suggesting that partial cell autolysis is not required for DNA degradation.

153 The dynamic equilibrium between growth and autolysis is perturbed by the presence of the antimicrob

154 Release of DNA without detectable autolysis is suggested to be an adaptation to the compet

155 Concomitant with autolysis is the generation of an N-terminally truncated

156 ivated proteases in post-necrotic myocardial autolysis is well characterized, their importance in hom

157 the surface of intact bacteria ("altruistic autolysis"), is essential for survival of H. pylori in a

158 Thus, HQNO-driven autolysis links programmed cell death with quorum sensin

159 ed enzyme: the N-terminus (Gly14-Gly19), the autolysis loop (Gly142-Thr154), and the 180s loop (Pro18

160 The autolysis loop (residues 143-154 in chymotrypsinogen num

161 ognition site for interaction with the basic autolysis loop (residues 143-154) of fXa.

162 Because the autolysis loop affects fibrinogen binding, but not prote

163 ut accelerated CTRC-mediated cleavage of the autolysis loop and did not protect against the detriment

164 ed that Arg-143, Lys-147, and Arg-154 of the autolysis loop and Lys-96, Lys-169, and Lys-236 of the h

165 Conversely, the autolysis loop and sodium-binding site exchanged more re

166 Modeling indicated steric proximity of the autolysis loop and the activation peptide in trypsinogen

167 f fXa that 1) contained substitutions in the autolysis loop and the heparin binding exosite, 2) lacke

168 n additional cleavage site at Leu-148 in the autolysis loop and the lack of the conserved Cys-139-Cys

169 esults suggest that Arg143 and Lys147 of the autolysis loop are recognition sites for FX independent

170 arkable feature of the structure is that the autolysis loop assumes a helical conformation enabling W

171 We found that mesotrypsin cleaved in the autolysis loop by CTRC exhibited catalytic impairment on

172 croM), IXaE245V 225 microM (240 microM), and autolysis loop cleaved IXaE245V 330 microM (350 microM).

173 To investigate the role of this autolysis loop in fX function, a recombinant variant wit

174 indicate that CTRC-mediated cleavage of the autolysis loop in mesotrypsin decreases protease activit

175 bition of autoactivation via cleavage of the autolysis loop is the dominant mechanism that can mitiga

176 e autolysis loop; and (d) proteolysis in the autolysis loop leads to a loss of catalytic efficiency w

177 ptide in trypsinogen, suggesting the cleaved autolysis loop may directly interfere with activation.

178 These results suggest that Arg(150) of the autolysis loop may specifically interact with the activa

179 The autolysis loop occupies a position up to 10 A closer to

180 The so-called autolysis loop of APC (residues 301-316, equivalent to c

181 zed that differences in the structure of the autolysis loop of coagulation proteases (residues 143-15

182 s suggest that structural differences in the autolysis loop of coagulation proteases play a key role

183 ge at Arg-318-Ser-319 in the protease domain autolysis loop of factor IXa results in its diminished b

184 The autolysis loop of factor Xa (fXa) has four basic residue

185 Previously, we identified Arg(150) on the autolysis loop of FXa as a candidate residue that may sp

186 ts demonstrate that basic amino acids in the autolysis loop of fXIa are important determinants of ser

187 rg318-Ser319[150-151] in the protease domain autolysis loop of IXaE245V with a concomitant loss of co

188 aved the Phe-150-Gly-151 peptide bond in the autolysis loop of T8 and T9 and inhibited autoactivation

189 g-144, Lys-145, Arg-147, and Lys-149) in the autolysis loop of the coagulation protease factor XIa (f

190 ymotrypsin that attacks W468 in the flexible autolysis loop of the protease domain in the open but no

191 The autolysis loop of thrombin comprises nine residues, from

192 shown that deletion of nine residues in the autolysis loop of thrombin produces a mutant with an ant

193 to thrombin-ABE I ligand complexes with the autolysis loop often most affected.

194 6A, 311A, 312A, and 314A) suggested that the autolysis loop provides for up to 15-fold discrimination

195 sumes an unprecedented conformation with the autolysis loop shifted 20 Angstroms away from its canoni

196 Des-44-Xa in which the autolysis loop was cleaved possessed </=5% of the amidol

197 Phe137 in euphauserase, localized in loop D (autolysis loop), is highly exposed on the surface of the

198 wo distinct exosites of fXa (36-loop and the autolysis loop).

199 tion of rTAP with the P2-binding pocket, the autolysis loop, and the Na(+)-binding loop is primarily

200 e, the Na(+) binding loop, the 186-loop, the autolysis loop, exosite I, and exosite II.

201 ected regions include segments of ABE-I, the autolysis loop, the edge of the active site region, and

202 ins are post-translationally sulfated in the autolysis loop, we also assessed the effect of this modi

203 mbined with deletion of nine residues in the autolysis loop, which by itself shifts the specificity o

204 The autolysis loop, which is disordered in the uninhibited f

205 .26 nM); IXaE245V, 2.5 microM (1.35 nM); and autolysis loop-cleaved IXaE245V, 15.6 microM (14.3 nM).

206 Additionally, autolysis loop-cleaved, active site-blocked native facto

207 he backbone away from exosite I and over the autolysis loop.

208 -332-Gln-333(150-151) in the protease domain autolysis loop.

209 rmful effects of mesotrypsin by cleaving the autolysis loop.

210 e of the Leu-149-Ser-150 peptide bond in the autolysis loop.

211 ately 3-fold and prevents proteolysis in the autolysis loop; and (d) proteolysis in the autolysis loo

212 The autolysis loops (amino acids 143-154, chymotrypsinogen n

213 The zymogen positioning of both the 180s and autolysis loops are synergistic structural elements that

214 To test this hypothesis, the autolysis loops of both thrombin and the anticoagulant s

215 The mechanism of autolysis may involve activation of the endogenous proph

216 elements differentiate through a specialized autolysis mechanism.

217 This is consistent with our altruistic autolysis model in which H. pylori uses genetically prog

218 It also suggested that autolysis occurs at Glu-729-Val-730 and Glu-732-Ala-733

219 lysis of a calpain substrate alpha-spectrin, autolysis of activated calpain, and reduction of cell da

220 ish fillets was carried out by assessment of autolysis of cells using a cytosolic enzyme lactate dehy

221 However, autolysis of human cationic trypsin is very slow in vitr

222 the MurA protein is involved in generalized autolysis of L. monocytogenes.

223 nactivation of arlRS does not play a role in autolysis of methicillin-resistant S. aureus (MRSA) stra

224 by crystallography and NMR spectra, prevents autolysis of MMP-12 and allows us to determine its NMR s

225 lood culture bottles may be difficult due to autolysis of pneumococci.

226 during high pressure homogenization-induced autolysis of Saccharomyces bayanus wine yeasts, treated

227 e importance of mgrA and sarA in controlling autolysis of Staphylococcus aureus, with MgrA and SarA b

228 ntration was greater than 40 microM, whereas autolysis of the 30-kDa subunit did not occur until the

229 Autopsy revealed autolysis of the kidneys and submassive bridging necrosi

230 has been suggested to play a pivotal role in autolysis of the parasitic cell wall of Coccidioides imm

231 proteolytic fragments (including those from autolysis of the protease) can be severe, due to high pr

232 strated that NEG can be generated during the autolysis of the yeast used in the preparation of the ye

233 The autolysis of the ypfP::cat mutant in the presence of 0.0

234 the beta-lactam resistance, growth, and cell autolysis of wild-type strain ATCC 9790 and resistant st

235 PH seemed a promising technique for inducing autolysis of wine yeasts.

236 Establishing the mechanisms regulating the autolysis of xylem tracheary elements (TEs) is important

237 its inactive form, intermolecular cleavage (autolysis) of AtMCP2d could also occur under our assay c

238 uring gonococci by two different mechanisms: autolysis or type IV secretion.

239 ly has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanis

240 ly has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanis

241 ent were observed within the first 60 min of autolysis (p>0.05), but subsequently increased up to 150

242 ctionality (via delta-lysin production), and autolysis phenotypes were assessed in MRSA isolates from

243 Autolysis plays an essential role in bacterial cell divi

244 Autolysis plays an important role in spoilage of fish an

245 of the catalytically inactive 43-kDa MT1-MMP autolysis product and decline in the TIMP-2 levels in co

246 hat 3,3'-diindolylmethane (DIM), a vegetable autolysis product, promoted Fas-mediated apoptosis of ch

247 e coverage up to 90% within minutes; trypsin autolysis products are not detected.

248 e apparent molecular weights as the in vitro autolysis products.

249 pression did not correlate directly with the autolysis profiles of single mgrA and sarA mutants.

250 XCP1 could be involved in tracheary element autolysis, promoter activity and localization of XCP1 we

251 motif did not prevent autoactivation but the autolysis rate was somewhat reduced.

252 yer, resulting in a dramatic decrease of the autolysis rate.

253 olysis, resulting in decreased and increased autolysis rates, respectively.

254 y to benzylpenicillin or the growth and cell autolysis rates.

255 mutants in many aspects including increased autolysis, reduced levels of surface-assembled tfp and d

256 g the most profoundly upregulated genes were autolysis-related genes and those that encode bacterioci

257 two TEV protease mutants, inactive C151A and autolysis-resistant S219D, have now been solved at 2.2-

258 factor regulatory genes, agr and sar, affect autolysis, resulting in decreased and increased autolysi

259 completely inhibited cell wall turnover and autolysis, resulting in the accumulation of cell wall ma

260 he design of an enzyme variant stabilized to autolysis should further the structural and mechanistic

261 MSP may be regulated in part by autolysis, since the active protein is readily inactivat

262 In addition, we identified an autolysis site between residues 95e and 95f in the 99-lo

263 g the herpesvirus proteases in possessing an autolysis site in the dimer interface, which removes the

264 An autolysis site of functional and structural significance

265 The autolysis site of the enzyme is separated from its catal

266 Four primary PR autolysis sites were blocked via substitution of either

267 se determined that blocking all four primary autolysis sites yielded a cleavage-resistant PR which wa

268 The fact that PQS levels correlated with autolysis suggests a fine balance in natural populations

269 would have a higher energy of activation for autolysis than chains aligned circumferentially.

270 rations (eg, thicker cell walls and abnormal autolysis) that are typical of in vivo VISA mutants.

271 , Pseudomonas aeruginosa is shown to control autolysis through the production of HQNO, a quorum-sensi

272 otenoid content was obtained with increasing autolysis time (p<0.05).

273 An autolysis time course determined that blocking all four

274 ne value (AV) of lipids were noticeable when autolysis time increased (p<0.05).

275 raction yield increased from 7.4% to 8.8% as autolysis time increased from 0 to 150 min.

276 distribution were not linked to age, gender, autolysis time, or subtype of schizophrenia.

277 hrenic subjects and their age-, gender-, and autolysis time-matched control subjects.

278 pylori uses genetically programmed bacterial autolysis to release urease and other cytoplasmic protei

279 we noted that it underwent autoproteolysis (autolysis) to give discrete cleavage products.

280 Unlike the classical autolysis, ultrasound led to a high cell disruption, and

281 Neisseria gonorrhoeae is prone to undergo autolysis under many conditions not conducive to growth.

282 grA is a pleiotropic regulator that controls autolysis, virulence, and efflux pump activity in Staphy

283 Autolysis was also postulated to play a protective role

284 ivity suggested that the observed incomplete autolysis was due to the ability of LLL to inhibit TE cy

285 Significant autolysis was induced in the Synechocystis sp. PCC 6803

286 ithin the intact central vacuole before mega-autolysis was initiated by tonoplast implosion.

287 ative stress; however, the extent of calpain autolysis was not altered.

288 pneumococci during log-phase growth, because autolysis was not believed to occur at this time.

289 than in the wild type) after the final mega-autolysis was otherwise complete.

290 biosynthesis, and in one suppressed mutant, autolysis was restored by addition of synthetic PQS.

291 Autolysis was suppressed by mutation of genes required f

292 Ser 4 His to stabilize the protease against autolysis) was determined to 2.0 A resolution in a new s

293 The rates of VacA secretion and autolysis were each influenced by medium composition, an

294 hepatopancreas without and with 60 min prior autolysis were observed.

295 activation, zymogen degradation, and trypsin autolysis were studied.

296 on of rpoN rendered E. faecalis resistant to autolysis, which in turn impaired eDNA release.

297 th, we examined the role of Gcp in bacterial autolysis, which is an important biological process for

298 A were more sensitive to a phosphate induced autolysis with respect to the untreated cells.

299 EASE1 (XCP1) and XCP2, participated in micro-autolysis within the intact central vacuole before mega-

300 ons did result in a significant reduction of autolysis without altering calpain proteolytic activity.