ライフサイエンスコーパス: auxotrophic

1 ryptophan, for which our wild-type strain is auxotrophic.

2 ndered A. baumannii and K. pneumoniae biotin auxotrophic.

3 thine transcarbamylase, making them arginine auxotrophic.

4 Plasmodium parasites are purine auxotrophic.

5 , whereas the DeltatyrA DeltafolX strain was auxotrophic.

6 Thus, an auxotrophic amber mutation in the pyrE2 gene can be comp

7 osome map was constructed by transduction of auxotrophic and cryptic transposon insertions, with know

8 loss of lgp-tubule formation identified one auxotrophic and nine prototrophic mutants.

9 Here we engineer auxotrophic and overexpression yeast strains that can be

10 orally administered live, attenuated, purine auxotrophic B. melitensis WR201 bacteria for their abili

11 ge, a crucial pathway for the survival of LA auxotrophic bacteria and parasites but non-essential in

12 These results establish UAA-auxotrophic bacteria as promising candidates for bacteri

13 here is also a strong stress response in the auxotrophic batch cultures, cell cycle arrest, if it occ

14 ineered bacteria, we devised a Cas9-assisted auxotrophic biocontainment system combining thymidine au

15 a previously uncharacterized mutant glycine auxotrophic cell line, rescued the glycine auxotrophy.

16 reening technology using bacterial phages or auxotrophic cell lines.

17 e to restore growth of Escherichia coli BCAA auxotrophic cells, but SlBCAT1 and -2 were less effectiv

18 activity using functional complementation of auxotrophic cells.

19 -depleting agent ADI-PEG20 in a non-arginine-auxotrophic cellular background (argininosuccinate synth

20 Arg-auxotrophic challenge also transcriptionally upregulates

21 s evaluated for its ability to complement an auxotrophic chorismate mutase deletion strain.

22 ols is essential for corals and other sterol-auxotrophic cnidarians.

23 roportions using cross-feeding in continuous auxotrophic co-culture.

24 omologous recombination utilizing the uracil auxotrophic COM1 parent strain (DeltapyrF).

25 ptation to temperature stress were probed by auxotrophic complementation of yeast with prokaryotic, t

26 lishing communities successfully obtained an auxotrophic composition in a nutrition-dependent manner,

27 odel of NP-C disease during anaerobiosis, an auxotrophic condition that requires yeast to utilize exo

28 Furthermore, the auxotrophic cultures express autophagy genes at substant

29 le mutant of E. coli rescues the d-glutamate auxotrophic defect.

30 s for its relative fitness compared to B(12)-auxotrophic diatoms and for the detection of B(12)-stres

31 rited the SXI2a-URA5 transgene became uracil-auxotrophic due to silencing of the URA5 gene.

32 Subsequent in vivo selection in an auxotrophic E. coli host yielded functional hybrid enzym

33 lected for by their ability to complement an auxotrophic E. coli strain.

34 We use mutually auxotrophic E. coli with different essential gene deleti

35 essfully tested for functional hybrids in an auxotrophic E.coli host strain.

36 rofolate reductase (mDHFR), in an isoleucine auxotrophic Escherichia coli host strain suspended in 5T

37 Complementation of a cysteine auxotrophic Escherichia coli strain NK3 led to the isola

38 incorporation was achieved using a cysteine auxotrophic Escherichia coli strain.

39 e metabolic gaps on a synthetic community of auxotrophic Escherichia coli strains.

40 ndosymbiosis, the marriage of an alga and an auxotrophic eukaryote.

41 SeM is readily introduced through auxotrophic expression and exhibits unique nucleophilic

42 Auxotrophic for 8 of the 10 essential amino acids, H. de

43 A cbiB mutant strain of Halobacterium was auxotrophic for adenosylcobinamide-GDP, a known de novo

44 archaeon Halobacterium sp. strain NRC-1 was auxotrophic for adenosylcobyric acid, a known intermedia

45 growth experiments, we found that S. suis is auxotrophic for Arg, Gln/Glu, His, Leu, and Trp in chemi

46 In vitro, S. gordonii is conditionally auxotrophic for arginine in monoculture but biosynthesis

47 CRhi cancer cells were auxotrophic for arginine, revealing a metabolic vulnerab

48 he disruption of which renders the bacterium auxotrophic for arginine, was expressed and its recombin

49 ular carcinomas (HCCs) have been shown to be auxotrophic for arginine.

50 ranscarbamoylase, several types of tumor are auxotrophic for arginine.

51 ininosuccinate synthetase (ASS1) making them auxotrophic for arginine.

52 species require exogenous B vitamins (i.e., auxotrophic for B vitamins), the possible importance of

53 We show that P. antarctica is not auxotrophic for B(12), as previously suggested, and iden

54 disruption mutants of ilvI and lrp were both auxotrophic for BCAA in CDM and attenuated compared to w

55 scenario in E. coli, L. lactis appears to be auxotrophic for biotin in that it lacks a full biotin bi

56 ies and auxotrophies, with most lichen fungi auxotrophic for biotin, most bacteria auxotrophic for th

57 mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids.

58 A lipL mutant is auxotrophic for carboxylic acid precursors required for

59 nlike land plants, many eukaryotic algae are auxotrophic for certain B vitamins.

60 Mutant bacteria that are auxotrophic for chitobiose have altered morphology, redu

61 we identify a subset of cancer cells that is auxotrophic for cholesterol and thus highly dependent on

62 rtain mutant Chinese hamster ovary cells are auxotrophic for cholesterol because they fail to carry o

63 plaining why many solid organ tumours become auxotrophic for cholesterol.

64 carrying a null allele of the cobY gene was auxotrophic for cobinamide-GDP, a known intermediate of

65 Disruption of cysH rendered Mtb auxotrophic for cysteine and methionine, and attenuated

66 trains containing null mutations in lipB are auxotrophic for either lipoic acid or octanoic acid.

67 ylserine (PtdSer) synthase/decarboxylase are auxotrophic for ethanolamine, which must be transported

68 ondrial function, filamentous growth, and is auxotrophic for ethanolamine.

69 metabolically engineered HAP1 cells that are auxotrophic for formaldehyde to find this cellular sourc

70 ARPE-19 cells became auxotrophic for growth on uridine after eight passages i

71 (P. falciparum nucleoside transporter 1) are auxotrophic for hypoxanthine, inosine, and adenosine und

72 detectable GS protein or GS activity and was auxotrophic for L-glutamine.

73 of the S. typhimurium A1-R mutant, which is auxotrophic for leu-arg and has increased antitumor viru

74 The S. typhimurium A1-R mutant, which is auxotrophic for leu-arg, is tumor-seeking and has antitu

75 to redox cycling agents and dioxygen and are auxotrophic for lysine when grown in air, and expression

76 ressed in an Escherichia coli strain that is auxotrophic for methionine and grown in the presence of

77 cobacterium tuberculosis metA mutant that is auxotrophic for methionine is unlike other auxotrophic m

78 HMG-CoA synthase gene rendered the organism auxotrophic for mevalonate and severely attenuated in a

79 an malaria parasite Plasmodium falciparum is auxotrophic for most amino acids.

80 Bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid.

81 ed an intracellular ornithine pool, and were auxotrophic for ornithine or polyamines.

82 nt promastigotes and axenic amastigotes were auxotrophic for polyamines and capable of robust growth

83 This Deltaspdsyn strain was auxotrophic for polyamines, required spermidine for grow

84 ender the insect vector form of the parasite auxotrophic for polyamines.

85 We then rendered the yeast auxotrophic for production of the donor nucleotide-sugar

86 rain, whereas the proC and trpD mutants were auxotrophic for proline and tryptophan, respectively.

87 he F. tularensis LVS delta purMCD mutant was auxotrophic for purines when grown in defined medium and

88 Leishmania are auxotrophic for purines, and consequently purine acquisi

89 ementation in Saccharomyces cerevisiae cells auxotrophic for purines, several highly functional noncy

90 ehyde 3-phosphate and E4P dehydrogenases are auxotrophic for pyridoxine.

91 ctron transport complex activities, and were auxotrophic for pyruvate and uridine.

92 s lacking a functional respiratory chain are auxotrophic for pyruvate, which serves as an exogenous e

93 Legionella pneumophila is auxotrophic for several amino acids including cysteine,

94 The symbionts appear to be auxotrophic for some vitamins, but have the potential to

95 Insects are auxotrophic for sterols; therefore, they have to consume

96 an obligate intracellular bacterium that is auxotrophic for the aromatic amino acids and histidine,

97 T. immunophila is auxotrophic for the bacterial cell wall amino sugar N-ac

98 All models were predicted to be auxotrophic for the vitamins niacin (vitamin B3) and thi

99 fungi auxotrophic for biotin, most bacteria auxotrophic for thiamine and the few annotated algae wit

100 hibition, suggesting that T. gondii might be auxotrophic for this cofactor.

101 l growth within human macrophages, which are auxotrophic for Trp and Phe, but is dispensable for grow

102 mutagenized using Tn5, and strains that were auxotrophic for tryptophan (Trp(-)) were isolated.

103 P. horikoshii is auxotrophic for tryptophan and histidine and is unable t

104 d altered stress response, in cells that are auxotrophic for tryptophan.

105 Yeast are auxotrophic for unsaturated fatty acids when grown under

106 fabB mutants were isolated; the mutants were auxotrophic for unsaturated fatty acids.

107 These cells lack mtDNA and are auxotrophic for uridine and pyruvate, which may explain

108 of the strains modeled were predicted to be auxotrophic for vitamins niacin (vitamin B3), thiamin (v

109 ment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+/matA-

110 cterized experimental model system, in which auxotrophic genotypes of E. coli derive essential amino

111 ocultures revealed that the mere presence of auxotrophic genotypes was sufficient to increase amino a

112 These auxotrophic GROs possess alternative genetic codes that

113 ants of yeast (Saccharomyces cerevisiae) and auxotrophic growth experiments.

114 incorporation of noncanonical amino acids in auxotrophic hosts allows the global exchange of a canoni

115 es or complete replacement of tyrosine using auxotrophic hosts remains the preferred method of introd

116 Streptococcus pyogenes, a multiple-auxotrophic human pathogen, regulates virulence gene exp

117 Such marker-free auxotrophic Leishmania cells show potential as an attenu

118 S. typhimurium A1 is auxotrophic (Leu/Arg-dependent) but apparently receives

119 A1 is auxotrophic (leucine-arginine dependent) but apparently

120 by evolving novel functional variants of an auxotrophic marker gene, and by conferring resistance to

121 Different auxotrophic marker genes can be used to separate co-clon

122 microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effec

123 y justified the status of MET15 as a classic auxotrophic marker, is largely explained by toxic accumu

124 d into the URA3 selectable/counterselectable auxotrophic marker.

125 Any of a variety of auxotrophic markers can recombine with each other, and t

126 The isolation of auxotrophic markers is important for molecular genetic s

127 However, the isolates differ in their auxotrophic markers, in the pI values of their dominant

128 In strains with several auxotrophic markers, loss of DOT4 ubiquitin hydrolase ac

129 etic manipulation of Bacillus subtilis using auxotrophic markers, the genes involved in alanine metab

130 The linear chromosome is almost devoid of auxotrophic markers, which probably explains why it was

131 prototrophs from strains with complementing auxotrophic markers; these contained both MTL alleles an

132 heme, the latter of high importance for heme auxotrophic members of the Bacteroidetes phylum.

133 esidues, introduced by Met replacement using auxotrophic Met(-) Escherichia coli strains, with glycoa

134 yers to our understanding of B(12) supply to auxotrophic microorganisms in the ocean and possibly in

135 or many years it has been known that thymine auxotrophic microorganisms undergo cell death in respons

136 Specifically, when yeast lysine-auxotrophic mutant lys- encountered lysine limitation, a

137 bicin from a monolayer of two cell lines: an auxotrophic mutant of Chinese hamster ovary cells, AUXB1

138 Here we report that an auxotrophic mutant of M. tuberculosis defective in the d

139 h phenotype and vaccine efficacy of a lysine auxotrophic mutant of Mycobacterium tuberculosis strain

140 A purine-auxotrophic mutant of P. aeruginosa was unable to replic

141 We cloned the PA gene into an auxotrophic mutant of Salmonella enterica serovar Typhim

142 We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201,

143 An adenine-requiring auxotrophic mutant strain of P. aeruginosa was isolated

144 We identified an auxotrophic mutant with a transposon insertion in the ge

145 Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were pro

146 xS, but not pdxT, resulted in a vitamin B(6) auxotrophic mutant.

147 rated for a community of four E. coli double auxotrophic mutants and is then applied to a gut microbi

148 allele resulted in the generation of leucine auxotrophic mutants by homologous recombination in 6% of

149 h the DeltaglnA1EA2 and DeltaglnA1 glutamine auxotrophic mutants conferred protection on C57BL/6 mice

150 By pairing auxotrophic mutants in co-culture, it has been demonstra

151 Auxotrophic mutants of Mycobacterium tuberculosis have b

152 When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mix

153 t by determining the symbiotic competence of auxotrophic mutants of the bioluminescent bacterium Vibr

154 We used auxotrophic mutants of the extremely thermoacidophilic a

155 s auxotrophic for methionine is unlike other auxotrophic mutants of this important species as methion

156 nes in the respective Escherichia coli m-DAP auxotrophic mutants restored the mutants to DAP prototro

157 tion for amino acids or other metabolites in auxotrophic mutants results in rapid loss of viability.

158 Growth media and auxotrophic mutants strongly influenced respiratory meta

159 serine was used to identify Escherichia coli auxotrophic mutants that exhibited limited growth in hum

160 Phagocytosis of the uracil auxotrophic mutants triggered a respiratory burst in neu

161 However, when attenuated, hyperinvasive auxotrophic mutants were used, the tumor-targeting and a

162 hrough the isolation and characterization of auxotrophic mutants, has emerged as a paradigm for the r

163 discussed from the development of tryptophan auxotrophic mutants, the molecular biology of conjugate

164 Tn-4351 insertions resulted in auxotrophic mutations and motility mutations.

165 date strain with two independent attenuating auxotrophic mutations in leucine and pantothenate biosyn

166 metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino

167 These findings indicate that neither auxotrophic mutations, nor vaccinia virus deficient for

168 ia with mevalonate permitted isolation of an auxotrophic mvaA null mutant that was attenuated for vir

169 CAT enzymes to store cholesterol in a sterol-auxotrophic organism such as Toxoplasma, and furthermore

170 targets for inhibitor design in these purine-auxotrophic organisms.

171 to defined media/feed as well as amino acid auxotrophic organisms.

172 Second, a putrescine auxotrophic, ornithine decarboxylase-deficient Chinese h

173 host metabolic proteins essential for these auxotrophic pathogens remains challenging.

174 flavin acquisition strategies by riboflavin auxotrophic pathogens remains poorly defined.

175 s proposed to inhibit the in vitro growth of auxotrophic pathogens.

176 Lyso-PtdCho supports the growth of a choline auxotrophic pem1Delta pem2Delta strain.

177 invasiveness was independent of the guanine auxotrophic phenotype and fully recovered when the chrom

178 bsence of polyamine supplementation, but the auxotrophic phenotype could be bypassed by addition of e

179 ivity in vitro and complement its methionine auxotrophic phenotype in vivo.

180 ty to complement the sn-glycerol-3-phosphate auxotrophic phenotype of a plsB mutant strain of E. coli

181 sense mutations are unable to complement the auxotrophic phenotype of met11(-) yeast cells and show l

182 as conducted for suppressors of the inositol auxotrophic phenotype of the ino4-8 mutant of yeast.

183 the SCON2 F-box, we have generated a sulfur auxotrophic phenotype previously unobserved in any scon-

184 found that deletion of bioW caused a biotin auxotrophic phenotype whereas deletion of bioI did not.

185 de 1) an assay for APLT function based on an auxotrophic phenotype, 2) direct demonstration of APLT a

186 treptococcus pneumoniae did not result in an auxotrophic phenotype.

187 observed striking differences in the lysine auxotrophic phenotypes of these three species of mycobac

188 ular metabolism of marine vitamin-requiring (auxotrophic) phytoplankton and bacteria would likely be

189 Earth Microbiome Project (EMP), and study of auxotrophic-prototrophic interactions in self-establishi

190 mutants of S. pneumoniae which have lost the auxotrophic requirement for choline and which were also

191 The auxotrophic requirement for cobinamide-GDP was corrected

192 6Chi and R6Cho(-)--that have lost the unique auxotrophic requirement of this bacterial species for ch

193 rows well in mineral salts medium and has no auxotrophic requirement.

194 a sole source of an amino acid to satisfy an auxotrophic requirement.

195 educed colony size, 4% had died out, 55% had auxotrophic requirements (increasing to 80% after 60 cyc

196 an utilize enkephalins to satisfy amino acid auxotrophic requirements for growth.

197 Starvation of yeast cultures limited by auxotrophic requirements results in glucose wasting and

198 wth of GBS, we assessed the stk1-mutants for auxotrophic requirements.

199 were regulated by the availability of their auxotrophic requirements.

200 report that an immediate-early event of Arg-auxotrophic response involves reactive oxygen species-me

201 ed multiple inter-regulatory pathways in Arg-auxotrophic response, consisting of Axl, c-Myc and ASS1,

202 systematically test pairwise combinations of auxotrophic Saccharomyces cerevisiae deletion mutants.

203 Here we use a model of auxotrophic Salmonella infection in germ-free mice to sh

204 Although live auxotrophic Salmonella no longer causes inflammation, it

205 Here, we used HeLa cells infected with an auxotrophic Salmonella to study the process of phagophor

206 The mice immunized with the auxotrophic Salmonella vaccine strain with the deletion

207 ementation of the culture medium of the heme-auxotrophic SCV with heme, but not iron, restored growth

208 e with the mutated gene to create an adenine-auxotrophic strain (LSU-E2).

209 wn amino acid biosynthesis to create a multi-auxotrophic strain and repressing stress responses to re

210 Because a sulfur auxotrophic strain is the only requirement of the approa

211 A methionine auxotrophic strain lacking CYS3 was able to grow in medi

212 to alkaliphilic Bacillus firmus OF4811M (an auxotrophic strain of B. firmus OF4) facilitated the ext

213 In this study, an adenine-auxotrophic strain of Edwardsiella ictaluri was construc

214 ntitative levels into proteins made in a Met-auxotrophic strain of Escherichia coli cultured in Met-d

215 We report here a modified auxotrophic strain of Salmonella typhimurium that can ta

216 li and by complementation of wdura5Delta, an auxotrophic strain of Wangiella dermatitidis (Wd) with a

217 est this concept, a budding yeast spermidine auxotrophic strain was found to grow almost normally wit

218 e free PABA and thus allows the growth of an auxotrophic strain.

219 we constructed an Escherichia coli glutamate auxotrophic strain.

220 organism, we have constructed ura3 and his3 auxotrophic strains by deleting the relevant coding regi

221 yl-tRNA synthetase (TrpRS) purified from six auxotrophic strains for changes in amino acid activation

222 odeficient individuals, we have studied five auxotrophic strains of BCG produced by insertional mutag

223 These results suggest that auxotrophic strains of BCG represent a potentially safe

224 to hemoglobin (Hb) by expressing the gene in auxotrophic strains of Escherichia coli.

225 d a collection of spontaneous mutants of DAP-auxotrophic strains of Mycobacterium smegmatis that can

226 s been shown to cause toxicity in tryptophan auxotrophic strains of yeast because this bioactive cera

227 d to design 61 unique multi-knockout E. coli auxotrophic strains that require significant metabolite

228 Three OptAux predicted non-specific auxotrophic strains-with diverse metabolic deficiencies-

229 bilities that indicate both prototrophic and auxotrophic strains.

230 ests that this gene product is absent in the auxotrophic strains.

231 -2H1,13C1]-tryptophan was fed to the Trp-His auxotrophic Streptomyces coelicolor strain WH101.

232 in these tumors and how they respond to Arg-auxotrophic stress are poorly understood.

233 nd gut) and in fermented food products, with auxotrophic taxa being relatively rare in soil and aquat

234 Auxotrophic taxa were more abundant in host-associated e

235 ormed by residues conserved among tryptophan-auxotrophic TDOs.

236 Because Plasmodium parasites are purine auxotrophic, they must import purines from their host to

237 Analysis of auxotrophic thiH mutants defined two classes, those that

238 two Salmonella vaccine strains attenuated by auxotrophic traits or by the regulated delayed attenuati

239 as been in clinical trials for targeting Arg auxotrophic tumors by Arg starvation therapy.

240 nine, may be a useful treatment for arginine auxotrophic tumors including melanoma and HCC.

241 s for improving the efficacy of treating Arg-auxotrophic tumors using Arg-deprivation strategies.

242 Many microorganisms are auxotrophic-unable to synthesize the compounds they requ

243 ding the production of recombinant vaccines, auxotrophic vaccines, DNA vaccines and subunit vaccines.

244 These results suggest that amino acid auxotrophic virulent bacteria, which selectively infect

245 We expressed PbENT1 in purine auxotrophic yeast and used radiolabeled substrate uptake

246 Complementation in auxotrophic yeast demonstrated reduced pyrimidine synthe

247 Inositol starvation of auxotrophic yeast interrupts glycolipid biosynthesis and

248 und that the deletion of OLE1 resulted in an auxotrophic yeast strain (designated OLE1 KO) that requi

249 Lysine auxotrophic yeast was grown in the presence of 13C-label

250 ain and compared them with those of a lysine auxotrophic yeast.