ライフサイエンスコーパス: auxotrophy

1 te to 3-phosphoglycerate, exhibited inositol auxotrophy.

2 re more sensitive to heat-induced methionine auxotrophy.

3 the SepRS-encoding gene resulted in cysteine auxotrophy.

4 cell wall synthesis and diaminopimelic acid auxotrophy.

5 synthesize asparagine and confers asparagine auxotrophy.

6 PDX2 in C. nicotianae results in pyridoxine auxotrophy.

7 ce the succinyl-CoA requirement relieved the auxotrophy.

8 loss of the URA3 marker and return to uracil auxotrophy.

9 but did not escape entirely from supplement auxotrophy.

10 desaturases are unable to complement the UFA auxotrophy.

11 nositol phospholipid metabolism and inositol auxotrophy.

12 protein receptors and second for cholesterol auxotrophy.

13 e pyrimidine biosynthetic pathway and uracil auxotrophy.

14 romycin B-resistant transformants for uracil auxotrophy.

15 produces temperature-sensitive myristic acid auxotrophy.

16 e; (ii) thermosensitive lethality; and (iii) auxotrophy.

17 ol methyl oxidase ERG25 gene leads to sterol auxotrophy.

18 gesterone toxicity resulted from cholesterol auxotrophy.

19 o exhibit yet another phenotype, an inositol auxotrophy.

20 phosphate pathway can rescue sod1 methionine auxotrophy.

21 reduction in flavin secretion rather than RF auxotrophy.

22 duced INO1 expression and conferred inositol auxotrophy.

23 equently, deletion of ilvA causes isoleucine auxotrophy.

24 deficient DHODH gene alleles induced uracil auxotrophy.

25 novo pyrimidine biosynthesis induced uracil auxotrophy.

26 donii and the basis for conditional arginine auxotrophy.

27 ankia microsymbionts to atypical patterns of auxotrophy.

28 f one or a few key genes have led to vitamin auxotrophy.

29 rulence in mice despite retaining pyrimidine auxotrophy.

30 rotein production costs and compensating for auxotrophy.

31 etic cost, and did not consider lifestyle or auxotrophy.

32 e auxotrophic cell line, rescued the glycine auxotrophy.

33 of FPP leading to growth defects and sterol auxotrophy.

34 t genetic linkage to swarming mutations, and auxotrophy.

35 nolamine dehydratase, PhhB, rescues tyrosine auxotrophy.

36 cell surface polysaccharides and a cysteine auxotrophy.

37 trains carrying one (CAI-4) to three (BWP17) auxotrophies.

38 e enzymes in sod1 Delta yeast results in the auxotrophies.

39 of the seo mutants to suppress the sod1Delta auxotrophies.

40 d reconstruction that prevent predictions of auxotrophies.

41 he known air-dependent methionine and lysine auxotrophies.

42 p-SRB1a partners of ANME-2c lack nutritional auxotrophies.

43 haracterized by smaller genomes and multiple auxotrophies.

44 ith numerous putative amino acid and vitamin auxotrophies.

45 microbes, thereby compensating for their own auxotrophies.

46 y, GEMs were used to predict strain-specific auxotrophies.

47 ven for a complex community of 14 amino acid auxotrophies.

48 we present evidence that the human Inositol auxotrophy 80 (Ino80) SNF2 ATPase is subject to regulati

49 Disruption of caa1 (+) resulted in aspartate auxotrophy, a finding that prompted us to assess the rol

50 rk, we quantify the prevalence of amino acid auxotrophies across a broad diversity of bacteria and ha

51 ylogenetically unrelated distribution of B12 auxotrophy across the algal lineages suggests that the M

52 a more complete understanding of amino acid auxotrophy across the bacterial tree of life and the eco

53 The complex pattern of vitamin auxotrophy across the eukaryotic tree of life is intimat

54 ic biocontainment system combining thymidine auxotrophy, an Engineered Riboregulator (ER) for control

55 me-scale metabolic reconstruction to predict auxotrophies and apply it to a series of available genom

56 To compensate for C. burnetii auxotrophies and other potential metabolic deficiencies,

57 The Deltacps lesion conferred pyrimidine auxotrophy and a growth requirement for medium supplemen

58 he 2-hybrid system, as assessed by histidine auxotrophy and beta-galactosidase activity, was disrupte

59 r Ni salts is markedly enhanced by histidine auxotrophy and by increasing the pH of the medium.

60 droxyl of the inositol ring, causes inositol auxotrophy and decreased intracellular inositol and phos

61 The gua mutations caused guanine/guanosine auxotrophy and led to partial derepression of direct Cod

62 ence of ancestral ODC and ornithine/arginine auxotrophy and link this with a known symbiotic dependen

63 dent mutant phenotypes, including methionine auxotrophy and oxygen sensitivity.

64 The P1 clone corrects the purine auxotrophy and protein deficiency of Chinese hamster ova

65 mitochondrial DNA was determined by uridine auxotrophy and quantitative real-time polymerase chain r

66 e lacking exon 1 overcame the cell's glycine auxotrophy and restored intracellular glycine concentrat

67 C; all of these characteristics, as well as auxotrophy and slow-growth rate, were reversed by transc

68 e that we have defined the cause of the glyB auxotrophy and that the glyB mft mutation identified a r

69 in clinical medicine whereby both amino acid auxotrophy and the immunoregulatory pathways controlled

70 n complementation of the strain's fatty acid auxotrophy and the production of Delta11Z-unsaturated fa

71 the M. smegmatis tam gene resulted in biotin auxotrophy, and addition of biotin to M. smegmatis cultu

72 imilation control protein (NAC) relieved the auxotrophy, and addition of compounds that could increas

73 y, one mutant (D335A) did not complement the auxotrophy, and another (R377A) allowed only minimal gro

74 iotaomicron, prevented escape from thymidine auxotrophy, and blocked transgene dissemination.

75 agenized the cells, selected for cholesterol auxotrophy, and identified two mutant cell lines (SRD-12

76 d by amplification phylotyping, nicotinamide auxotrophy, and outer membrane protein patterns (OMPs).

77 with respect to promoters affected, inositol auxotrophy, and Spt- phenotypes.

78 I; confer phosphate, galactose, and inositol auxotrophies; and fail to activate PHO5, GAL10, and INO1

79 By comparison, the sod1Delta lysine auxotrophy appears to be reversed in the seo mutants by

80 Our estimates indicate that amino acid auxotrophies are more prevalent among obligate intracell

81 ial-diatom model system based on vitamin B12 auxotrophy as a sensitive assay for metabolite exchange

82 hesis, in the pyc::tn strain reverted biotin auxotrophy, as did reconstituting the last step of the p

83 IDP1 partially compensated for the glutamate auxotrophy associated with loss of IDH.

84 owever, previous attempts to generate uracil auxotrophy by genetically deleting the mitochondrion-ass

85 of life and the ecological contexts in which auxotrophy can be a successful strategy.

86 ll wall peptides, we have found that proline auxotrophy can be satisfied with the peptide Pro-Phe-Lys

87 nce of inositol and exacerbates the inositol auxotrophy caused by deletion of SCS2.

88 fur fixation in yeast, in which organosulfur auxotrophy caused by deletion of the homocysteine syntha

89 expression rescued aco1 yeast from glutamate auxotrophy, cells remained growth-limited by glutamate,

90 elatively small genome and multiple putative auxotrophies characteristic of Ca. U. copiosus suggest t

91 oach uses yeast homologous recombination, an auxotrophy complementation-based yeast selection system

92 s, using a pangenome approach, and highlight auxotrophies conferring a fitness advantage in vivo.

93 Auxotrophies constrain the interactions of bacteria with

94 rowth in medium without polyamines; however, auxotrophy could be rescued by spermidine but not by put

95 5-diaminopentane (cadaverine), but polyamine auxotrophy could not be overcome by other aliphatic diam

96 Our results show that amino acid auxotrophies create additional interdependencies that de

97 ne biosynthesis, and in addition to cysteine auxotrophy, cys4 mutants have much lower levels of intra

98 capsulatum, resulting in nonreverting uracil auxotrophy due to a mutation in the URA5 gene.

99 We examined the effect of uracil auxotrophy due to a ura5 mutation on H. capsulatum virul

100 ments that led to complementation of thymine auxotrophy during intracellular growth of the bacterium

101 nd that several amino acids complemented the auxotrophy either by producing glutamate via transaminat

102 ts show that the clk-1 mutations result in Q auxotrophy evident only when Q is removed from the diet,

103 in central metabolic pathways, resulting in auxotrophies for biotin, proline, and arginine that were

104 ation of [O2-] imposes, on Escherichia coli, auxotrophies for branched chain, sulfur-containing, and

105 types, namely poor growth in air and aerobic auxotrophies for lysine and methionine.

106 toplasmic superoxide dismutase (SOD) exhibit auxotrophies for sulfur-containing, branched-chain, and

107 g, sporulation, stationary phase survival or auxotrophies for sulphur-containing amino acids.

108 Despite exhibiting auxotrophies for various amino acids, DeltadksA mutant S

109 creened for a range of phenotypes, including auxotrophy for amino acids, inability to reduce Fe(III)

110 racellular environment consistent with their auxotrophy for arginine.

111 Disruption of citH caused partial auxotrophy for aspartate and a requirement for aspartate

112 esponses to oxidative stress: 1) ROS-induced auxotrophy for branched-chain, aromatic, and sulfurous a

113 the current work we show an additional leaky auxotrophy for leucine.

114 The Deltaodc L. donovani exhibited an auxotrophy for polyamines that could be circumvented by

115 engineer Pseudomonas aeruginosa to maintain auxotrophy for the UAA p-benzoyl-L-phenylalanine (BzF) t

116 ic conditions the deletion of IscS caused an auxotrophy for thiamine and nicotinic acid, whereas unde

117 Auxotrophy for Val and Thr was confirmed by in vivo expe

118 ockout strains were evaluated for pyrimidine auxotrophy, for attenuation of virulence, and for their

119 failure to reduce SO3(2-) leads to cysteine auxotrophy, for which the enzyme is named.

120 dent methionine synthase, and that cobalamin auxotrophy has arisen numerous times throughout evolutio

121 llectively, our data demonstrate that uracil auxotrophy has cell type-specific effects on the fate of

122 The former two classes of auxotrophies have already been explained, whereas the th

123 affects all markers tested: three different auxotrophies (histidine, purine, and cobalamin) and resi

124 These phenotypes include inositol auxotrophy, impaired telomeric silencing, and synthetic

125 alanine completely eliminates the methionine auxotrophy imposed by diamide treatment, suggesting that

126 identify the metabolic and genetic basis for auxotrophies in Gram-negatives.

127 mal gene is capable of complementing leucine auxotrophy in a leuB(-) strain lacking the paralogous is

128 se) TAGKO cDNA failed to complement cysteine auxotrophy in a yeast CBS mutant.

129 ng constitutive promoter leads to methionine auxotrophy in B. subtilis, suggesting that S-adenosylmet

130 Using complementation of tyrosine auxotrophy in Escherichia coli as a functional test, we

131 studies of SCV S. maltophilia have suggested auxotrophy in hemin, methionine, and thymidine associate

132 a novel model for the evolution of metabolic auxotrophy in microorganisms that arises as a result of

133 This is the first description of DAP auxotrophy in mycobacteria.

134 , and are shown here to result in a thiamine auxotrophy in some of the strains tested, including S. e

135 Lesions in apbC also cause a thiamine auxotrophy in strains proficient in purine biosynthesis

136 itivity to oxidative stress, (ii) a thiamine auxotrophy in the absence of the YggX protein, and (iii)

137 for the widespread distribution of cobalamin auxotrophy in the algal kingdom.

138 ADOMETDC overexpression abrogated polyamine auxotrophy in the Delta adometdc L. donovani.

139 Mechanisms underlying Arg auxotrophy in these tumors and how they respond to Arg-a

140 ds, substantially, current knowledge of haem auxotrophy in ticks and underscores the importance of ha

141 Synthetic auxotrophy in which bacterial viability depends on the p

142 oxide dismutase (Sod1p) is an aerobic lysine auxotrophy; in the current work we show an additional le

143 met6) exhibits methionine as well as adenine auxotrophy indicating that MS is required for methionine

144 A fur mutation only partially relieved the auxotrophies, indicating that Fur derepression assists b

145 tion of P. aeruginosa cysH produced cysteine auxotrophy, indicating its role in sulfate assimilation.

146 H, and proI) were required to confer proline auxotrophy, indicating that the products of these genes

147 Overall, our findings reveal that synthetic auxotrophy is effective on time scales and in contexts t

148 conclude that when Sod1p is absent a lysine auxotrophy is induced because Lys4p is inactivated in th

149 We show that the metabolic basis of auxotrophy is species-dependent and varies with 1) pathw

150 Amino acid auxotrophy is widespread across bacterial phyla, but we

151 genetic make-up of the cell, as reflected in auxotrophy, is hence likely to be a determinant of gene

152 Given the indispensable role of heme, this auxotrophy may be exploited to develop drugs that interf

153 tant, mc21278 (ask1::aph), exhibits multiple auxotrophy (Met-, Thr-, DAP-, and Lys-) and is complemen

154 f haploid selection is mating-type-regulated auxotrophy (MRA), by which prototrophy is restricted to

155 or fermentable carbon and the branched-chain auxotrophy occur because superoxide (O2-) leaches iron f

156 t in opposition: (i) complete suppression of auxotrophy occurs by overexpression of GreA or DksA only

157 e the oxygen-dependent methionine and lysine auxotrophies of a sod1Delta strain.

158 antial evidence that the sulfur and aromatic auxotrophies of SOD mutants are also directly or indirec

159 es of ATX2 were found to reverse the aerobic auxotrophies of sod1(delta) mutants for lysine and methi

160 Overexpression of the alsK gene relieves the auxotrophy of a glucokinase-deficient bacterium, demonst

161 -Huet anomaly fail to rescue the cholesterol auxotrophy of a LBR-deficient human cell line, consisten

162 DksA overexpression partially suppresses the auxotrophy of a ppGpp-deficient strain; (iii) microarray

163 icum panD(+) gene corrected the pantothenate auxotrophy of a S. enterica yhhK strain, supporting in v

164 and was capable of suppressing the inositol auxotrophy of a second ino4 missense mutant, ino4-26, as

165 active (i.e. failed to rescue the methionine auxotrophy of a shm2Delta ade3 strain) complemented the

166 e ability of PfPMT to complement the choline auxotrophy of a yeast mutant defective in phospholipid m

167 auxotrophy, similar to the adenine/histidine auxotrophy of ade3 mutant yeast strains.

168 This work reveals that the cholesterol auxotrophy of ALCLs is a targetable liability and, more

169 thaliana is able to complement the cysteine auxotrophy of an Escherichia coli cysH [3'-phosphoadenos

170 complement the unsaturated fatty acid (UFA) auxotrophy of an Escherichia coli fabA/fadR mutant.

171 aromyces cerevisiae mutant to model the heme auxotrophy of C. elegans and demonstrate that, under hem

172 The subcloned cDNA complemented the glycine auxotrophy of glyB cells and reinstated folate accumulat

173 ne suppresses the choline-sensitive inositol auxotrophy of mpk1Delta cells, whereas overexpression of

174 ver, M+ mutations, which suppress amino acid auxotrophy of ppGpp degrees strains and which have been

175 f PpMS and its ability to reverse methionine auxotrophy of Ppmet6 Thus, association of two PpMS monom

176 is unable to reverse methionine and adenine auxotrophy of Ppmet6 Thus, nuclear localization is essen

177 pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does n

178 hat rescues the sphingolipid long-chain base auxotrophy of Saccharomyces cerevisiae SPT mutants when

179 ns explain the characteristic nicotinic acid auxotrophy of Shigella organisms and are consistent with

180 r, these findings indicate that the inositol auxotrophy of snf1Delta strains arises in part from elev

181 as uncovered as a suppressor of the inositol auxotrophy of snf1Delta strains.

182 ic selection for suppressors of the inositol auxotrophy of snf1Delta strains.

183 expression of ACC1, suppresses the inositol auxotrophy of snf1Delta strains.

184 temperature-sensitive unsaturated fatty acid auxotrophy of strain M6 [fabA6 (Ts)] were isolated from

185 ic C1-THF synthase to complement the adenine auxotrophy of the ade3 deletion strain.

186 the medium did not circumvent the polyamine auxotrophy of the Deltaarg line.

187 e or orotate could circumvent the pyrimidine auxotrophy of the Deltacps/Deltauprt double knockout.

188 (ino1Delta/hINO1) complemented the inositol auxotrophy of the mutant and led to inositol excretion.

189 xtrachromosomal promoter, can complement the auxotrophy of the organism.

190 The biotin auxotrophy of the pyc::tn strain is due to failure to tr

191 gene are both able to complement the choline auxotrophy of the S. pombe cho1 mutants.

192 Liberobacter solanacearum complement the RF auxotrophy of the Sm1021DeltaSMc02977 mutant.

193 ated that specifically suppress the inositol auxotrophy of the TBP mutant strains.

194 ate lyase mRNA, suggesting that the arginine auxotrophy of these cells was a result of an inability t

195 , resulted in the complementation of glycine auxotrophy of these cells.

196 n of HGT1 in gsh1Delta cells rescues the GSH auxotrophy of this strain.

197 accumulation and partially rescue the lysine auxotrophy of yeast grx5 cells.

198 udy, we determined the effect of nutritional auxotrophy on the ability of L. pneumophila to survive i

199 in sensitivity to ampicillin, in nutritional auxotrophies, or temperature-sensitive growth were mappe

200 lighting functions of enzymes, and substrate auxotrophy owing to the disruption of pathways that enab

201 ygenase expression as a cause of cholesterol auxotrophy, particularly in ALK(+) anaplastic large cell

202 intracellular replication was due to leucine auxotrophy per se and not to a polar effect of the trans

203 The polyamine auxotrophy, polyamine-independent growth and biofilm for

204 pSer bioavailability but also imposes serine auxotrophy, preventing growth in minimal media used for

205 While synthetic auxotrophy previously prevented Escherichia coli from ex

206 g and physical containment of microbes using auxotrophies, regulation of essential genes, and express

207 nzymatically inactive gdhA1 allele showed no auxotrophy, repression of GDH expression by the nitrogen

208 of enough functional product to overcome an auxotrophy resulting from a nonsense mutation.

209 a coli WP2 bacteria with an ochre amino acid auxotrophy show no evidence of growth during the first f

210 17 double disruption also leads to histidine auxotrophy, similar to the adenine/histidine auxotrophy

211 to be a bradytrophy, rather than an absolute auxotrophy, since lack of Cys merely imposed a growth la

212 The suppressor of long chain base auxotrophy (SLC, strain 7R4) showed a heat-sensitive phe

213 d research, strategies to harness amino acid auxotrophy so as to block cancerous lymphocyte growth ha

214 e for mutants that suppress lethality during auxotrophy starvation.

215 25% of the insertions resulted in amino acid auxotrophy, suggesting that insertion was random at a gr

216 tructed stable haploid strains with multiple auxotrophies that will facilitate molecular and genetic

217 how that loss of Ino1 results in an inositol auxotrophy that can be rescued only partially by exogeno

218 inally, disruption of carB leads to multiple auxotrophy that prevents lgp-tubule formation.

219 e TPP (null mutants) and those with thiamine auxotrophy that was corrected by either L-tyrosine or th

220 high-copy-number clones of gdhA displayed an auxotrophy that was interpreted as a limitation for alph

221 ion, inducible toxin switches and engineered auxotrophies, these approaches are compromised by cross-

222 e combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.

223 Using Northern analysis, we have traced this auxotrophy to a complete shutdown of cys-3+ gene express

224 which enables the selection of organosulfur auxotrophy to better tune cell growth to the metabolic e

225 Here we show, by exploiting this auxotrophy to identify HRG-1 proteins in C. elegans, tha

226 ic assay based on phenotypic conversion from auxotrophy to prototrophy was established to select effi

227 c capacity and exploitation of the resulting auxotrophy to signal a particular host environment.

228 of visceral leishmaniasis, confer polyamine auxotrophy to the insect vector or promastigote form of

229 investigate environmental associations with auxotrophy, using data compiled from 3813 samples spanni

230 that suppression of the sod1Delta methionine auxotrophy was dependent on the pentose phosphate pathwa

231 The predicted niacin auxotrophy was experimentally verified, as well as the e

232 The CCR-mediated proline auxotrophy was lifted when nonpreferred carbohydrates we

233 hiamin (vitamin B1), whereas strain-specific auxotrophies were predicted for riboflavin (vitamin B2),

234 ld type in many types of media even when the auxotrophies were satisfied.

235 ve growth phenotype of pos5 and its arginine auxotrophy were repaired by plasmid-borne POS5 but not U

236 it of the dehydrogenase) exhibit a glutamate auxotrophy when ammonium is the sole source of nitrogen.

237 h on minimal medium but resulted in thiamine auxotrophy when exogenous purines were supplied.

238 hat it can repair the unsaturated fatty acid auxotrophy when it is expressed in a Saccharomyces scspt

239 ese two chromosomal genes results in adenine auxotrophy, whereas expression of either gene alone is s

240 Deletion of OMPDC induced a severe uracil auxotrophy with loss of replication, loss of virulence i

241 hat synchronization of bacterial amino acids auxotrophy with the host is a driving force in pathogeni

242 est interdigitated vitamin prototrophies and auxotrophies, with most lichen fungi auxotrophic for bio

243 epletion that was specifically manifested as auxotrophy within PIK3CA mutant cells.

244 pathway exhibit a choline-sensitive inositol auxotrophy, yet fully derepress INO1 and other Opi1p-reg

245 linositol 4,5-bisphosphate, exhibit inositol auxotrophy, yet fully derepress INO1, encoding inositol-

246 As) provide a strategy to engineer stringent auxotrophies, yielding conditionally replication incompe