ライフサイエンスコーパス: axolotl

1 f Ambystoma texanum and Ambystoma mexicanum (axolotl).

2 arch, similar to that seen in zebrafish and axolotl.

3 ) signaling regulate limb development in the axolotl.

4 d in mesenchymal Amex.Fgf8 expression in the axolotl.

5 mesenchymal Amex.Fgf8 expression seen in the axolotl.

6 to interrogate neuronal organization in the axolotl.

7 egeneration and translational control in the axolotl.

8 g genes for traits in the laboratory Mexican axolotl.

9 -1 (NRG1) fulfills all these criteria in the axolotl.

10 chyury in Xenopus, it activates Brachyury in axolotl.

11 including 1 of the more popular models: the axolotl.

12 tion of postcranial mesoderm in catshark and axolotl.

13 ontrol over exogenous gene expression in the axolotl.

14 ment may participate in limb regeneration in axolotls.

15 generation of the body axis and the limbs of axolotls.

16 efficient overexpression of foreign genes in axolotls.

17 s of both neotenous and metamorphosing adult axolotls.

18 skeletal and cardiac muscle of adult Mexican axolotls.

19 patterns during limb development in neotenic axolotls.

20 landscape of the neotenic and metamorphosed axolotls.

21 on that drives enhanced limb regeneration in axolotls.

22 ional identity to guide limb regeneration in axolotls.

23 ver of regenerative spinal cord outgrowth in axolotls.

24 ced Cre-mediated recombination in transgenic axolotls.

25 heckpoint kinases Chk1 and Chk2 in wild-type axolotls.

26 Although ampullary organs in the axolotl (a representative of the lobe-finned clade of bo

27 The molecular genetic toolkit of the Mexican axolotl, a classic model organism, has matured to the po

28 ng of the blastema over a time course in the axolotl, a species whose genome has not been sequenced.

29 The axolotl, a urodele amphibian, provides a model with all

30 er demonstrate that, upon limb amputation in axolotls, a complex array of filopodial extensions is fo

31 odeled structure, and function indicate that axolotl AHR binds TCDD weakly, predicting that A. mexica

32 Axolotl AHR bound one-tenth the TCDD of mouse AHR in vel

33 in the redifferentiated limb tissues in the axolotl, Amblystoma mexicanum, and in Notophthalmus viri

34 apods, only urodele salamanders, such as the axolotl Ambystoma mexicanum, can completely regenerate l

35 cords and dorsal root ganglia of Xenopus and axolotl (Ambystoma mexicanum) axons grow directly to the

36 signed to target epigenetic mechanisms in an axolotl (Ambystoma mexicanum) embryo tail regeneration a

37 The Mexican axolotl (Ambystoma mexicanum) has a derived mode of deve

38 The Mexican axolotl (Ambystoma mexicanum) is a well-established tetr

39 The Mexican axolotl (Ambystoma mexicanum) is capable of fully regene

40 en Mendelian mutants have been discovered in axolotl (Ambystoma mexicanum) populations, including sev

41 The axolotl (Ambystoma mexicanum) possesses a remarkable abi

42 The axolotl (Ambystoma mexicanum) provides critical models f

43 After appendage amputation, the axolotl (Ambystoma mexicanum) regenerates missing struct

44 t of regenerating limb tissue in the Mexican axolotl (Ambystoma mexicanum) that is indicative of cell

45 ate the organization of genes in the Mexican axolotl (Ambystoma mexicanum), a species that presents r

46 Using similar strategies in the Mexican axolotl (Ambystoma mexicanum), and the South African cla

47 We fate-map this mesoderm in the axolotl (Ambystoma mexicanum), which retains external gi

48 sponse critical for limb regeneration in the axolotl (Ambystoma mexicanum).

49 We isolated an AHR cDNA from the Mexican axolotl (Ambystoma mexicanum).

50 to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral path

51 We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, col

52 immune signaling during limb regeneration in axolotl, an aquatic salamander, and reveal a temporally

53 rce to explore the molecular identity of the axolotl and facilitates better understanding of metamorp

54 from both human and the highly regenerative axolotl and found that the harmonized atlas also improve

55 also inhibits cell migration in vitro using axolotl and human fibroblasts.

56 Comparison of AP-2 expression in axolotl and lamprey suggests an elaboration of cranial n

57 s study we cloned germline VH genes from the axolotl and obtained rearrangements to these VH gene seg

58 esolving genome-wide orthologies between the axolotl and other vertebrates, identifying the footprint

59 NA-seq analysis of regenerating blastemas of axolotl and Polypterus reveals the activation of common

60 nce allows a reverse genetic approach in the axolotl and will undoubtedly provide invaluable insight

61 We hypothesize that this characteristic of axolotl and Xenopus AHRs arose in a common ancestor of t

62 long-term fate mapping using GFP-transgenic axolotl and Xenopus laevis to document the contribution

63 both active sites of hematopoiesis in adult axolotls and contain transplantable HSCs capable of long

64 Axolotls and other salamanders can regenerate entire lim

65 Using transgenic assays in zebrafish, axolotl, and mouse, we discover three conserved Brachyur

66 luding Old and New World monkeys, seahorses, axolotls, and Xenopus.

67 We used an axolotl animal cap system to demonstrate that signalling

68 domain and is expressed as a monomer in the axolotl animal cap.

69 ion and comparison of amphioxus, lamprey and axolotl AP-2 reveals its extensive expansion in the vert

70 rating limb in tetrapods such as the Mexican axolotl are unknown.

71 Axolotls are amphibian models for studying nervous syste

72 Axolotls are an important model organism for multiple ty

73 Axolotls are poised to become the premiere model system

74 llular changes in neotenic and metamorphosed axolotls are still poorly investigated.

75 Axolotls are unique in their ability to regenerate the s

76 Axolotls are uniquely able to mobilize neural stem cells

77 Axolotls are uniquely able to resolve spinal cord injuri

78 entified, we cloned a DAZ-like sequence from axolotls, Axdazl.

79 d Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo.

80 eport the isolation of a Nanog ortholog from axolotls (axNanog).

81 ated mutations in the limbs of mosaic mutant axolotls before and after regeneration and found that th

82 rove invaluable for studying many aspects of axolotl biology.

83 of Drosophila Dll, has been isolated from an axolotl blastema cDNA library, and its expression in dev

84 ral patterns in mouse digit regeneration and axolotl blastema differentiation reveals common gene gro

85 uencing (RNA-seq) analysis of Polypterus and axolotl blastemas to provide support for a common origin

86 AAVs for efficient gene transfer within the axolotl brain, the spinal cord, and the retina.

87 y and inhibitory neuronal populations of the axolotl brain.

88 lly functional neurons can be regenerated in axolotls, but challenge prior assumptions of functional

89 tory throughout the year, for metamorphosing axolotls by a single i.p. injection and for axolotl tran

90 munofluorescent staining using an Ab against axolotl C3 and by in situ hybridization with an axolotl

91 lotl C3 and by in situ hybridization with an axolotl C3 cDNA probe.

92 The axolotl can regenerate multiple organs, including the br

93 Axolotls can regenerate lost limbs throughout life, whil

94 upon mechanical injury to the adult pallium, axolotls can regenerate several of the populations of ne

95 Remarkably, axolotls can repair their spinal cord after a small lesi

96 in biology is why some species, such as the axolotl, can regenerate tissues whereas mammals cannot(1

97 This distinguishing feature of axolotl CDR3 results not only from shorter junctional se

98 olution of transcription factors in a single axolotl cell and compare numerical simulations with prev

99 ross 19 tissues to construct the first adult axolotl cell landscape.

100 d by changing their coat protein, can infect axolotl cells only when they have been experimentally ma

101 AxFUCCI axolotls confirmed the predicted appearance time and siz

102 Only 29% of the CDR3 loop in the axolotl consisted of somatically generated sequences, co

103 enome, and transformation of abundances from axolotl contigs to human genes.

104 PouV domain proteins from both Xenopus and axolotl could support murine ES cell self-renewal but th

105 Together with the axolotl data, this confirms that ampullary organs are an

106 xception to this paradigm is the salamander (axolotl) developing and regenerating limb, where key Fgf

107 Since axolotls do not form an expanded paddle-like handplate p

108 tream components were found expressed in the axolotl ectoderm, indicating that they are not direct re

109 d unique cell lineages within the developing axolotl embryo and tracked the frequency of each lineage

110 assessment of Hsp90alpha modulators in a new axolotl embryo tail regeneration (ETR) assay as a potent

111 oropharyngeal region was taken from a donor axolotl embryo, prior to its innervation and development

112 ng a homeobox gene, AxNox-1, from a stage 18 axolotl embryonic cDNA library which shows only moderate

113 inding of migrating pronephric duct cells in axolotl embryos by: (1) demonstrating that application o

114 We conclude that elongation of axolotl embryos requires active cell rearrangements with

115 cific phospholipase C to early tailbud stage axolotl embryos reveals that a specific subset of morpho

116 trulation were manipulated systematically in axolotl embryos, and the subsequent ability of the phary

117 force driving anteroposterior stretching in axolotl embryos, elongation of other tissues being a pas

118 x are required for mesoderm specification in axolotl embryos, suggesting the ancestral vertebrate sta

119 is idea, we devised a culture approach using axolotl embryos.

120 ents that occurred during the development of axolotl genetic stocks, and precisely mapping several ph

121 ormation and for the first time assemble the axolotl genome into 14 chromosomes.

122 hromosome-scale assembly of the giant, 32 Gb axolotl genome.

123 reate mutations at targeted sites within the axolotl genome.

124 These results suggest that, in axolotls, germ plasm components are insufficient to spec

125 The mesenchymal expression of Amex.Fgf8 in axolotl has been suggested to be critical for regenerati

126 ncluding its best-known species, the Mexican axolotl, has long been a source of biological fascinatio

127 osin cDNAs designated ATmC-1 and ATmC-2 from axolotl heart tissue and one TM cDNA from skeletal muscl

128 t ATmC-2 is expressed predominantly in adult axolotl hearts.

129 However, a lack of knowledge of axolotl hematopoiesis hinders the use of this animal for

130 embly will greatly facilitate studies of the axolotl in biological research.

131 rotocol for successfully mating and breeding axolotls in the laboratory throughout the year, for meta

132 es primordial germ cell (PGC) development in axolotls, in which PGCs are maintained by an extracellul

133 nder complex are endangered, and the Mexican axolotl is an important model system in regenerative and

134 e axial position of the head-trunk border in axolotl is congruent between LPM and somitic mesoderm, u

135 e analog, IPTG, to the swimming water of the axolotl is sufficient for the sugar to be taken up by ce

136 Our results show that gene expression in axolotls is diverse and precise, and that axolotls provi

137 salamander Ambystoma mexicanum (the Mexican axolotl) is a model organism for studies of regeneration

138 ries of several chordates including chicken, axolotl, lamprey, Ciona, and amphioxus, revealing a univ

139 promoter constructs were electroporated into axolotl limb blastemas and the wild type promoter was mo

140 us blastema to single-cell RNA-seq data from axolotl limb bud and limb regeneration stages shows that

141 sayed by nucleofecting AL1 cells, a cultured axolotl limb cell line that expresses both Prod 1 and Me

142 We found that Amex.Wnt3a is expressed in axolotl limb epidermis, similar to chicken and mouse.

143 t have been described, TH greatly stimulates axolotl limb growth causing the resulting larva to be pr

144 ng what controls Amex.Fgf8 expression in the axolotl limb mesenchyme.

145 rns in our yeast osmotic stress response and axolotl limb regeneration case studies.

146 Early events during axolotl limb regeneration include an immune response and

147 acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vas

148 as well as the wound epidermis, during early axolotl limb regeneration.

149 ding question of why nerves are required for axolotl limb regeneration.

150 nt and inflammation during the initiation of axolotl limb regeneration.

151 we combined optical clearing of whole-mount axolotl limb tissue with single molecule fluorescent in

152 transcriptional dynamics of the regenerating axolotl limb with respect to the human gene set.

153 ar density along the PD axis of regenerating axolotl limbs after transfecting distal blastemas with T

154 Axolotl limbs offer an opportunity to distinguish these

155 Remarkably, neotenic axolotls may undergo metamorphosis, a process that trigg

156 tudied PGC specification in embryos from the axolotl (Mexican salamander), a model for the tetrapod a

157 The axolotl (Mexican salamander, Ambystoma mexicanum) has be

158 In axolotl models, it not only records neural electrical ac

159 By engineering an axolotl mTOR (axmTOR) in human cells, we show that these

160 This change renders axolotl mTOR more sensitive to nutrient sensing, and inh

161 iplex CRISPR/Cas9 haploid screen in chimeric axolotls (MuCHaChA), which is a novel platform for haplo

162 As in zebrafish, use of the white axolotl mutant allows direct visualization of homing, en

163 Axolotl NANOG is absolutely required for gastrulation an

164 stem but virus-mediated gene delivery to the axolotl nervous system has not yet been described.

165 ols to visualize and manipulate cells of the axolotl nervous system with high-efficiency, spatial and

166 ditional components must be important in the axolotl network in the specification of the full range o

167 Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and r

168 er, mouse TMEM16A and TMEM16B yield CaCCs in Axolotl oocytes and mammalian HEK293 cells and recapitul

169 Using Axolotl oocytes as an expression system, we have identif

170 Axdazl RNA is not localized in axolotl oocytes, and, furthermore, these oocytes do not

171 m liver nuclei following their transfer into axolotl oocytes.

172 Osprey Optimization Algorithm (OOA), Mexican Axolotl Optimization (MAO), Single candidate optimizer (

173 Here, we investigated how the axolotl ortholog of NANOG programs pluripotency during d

174 Here we show that the axolotl pattern is strikingly similar to that in amniote

175 ion factors, we demonstrate that, unlike the axolotl, Pax3 is present and necessary for development a

176 In the axolotl, PGCs develop within mesoderm, and classic studi

177 to identify molecular bases for two historic axolotl pigment phenotypes: white and albino.

178 results suggest that commercial and hobbyist axolotl populations may harbor useful mutants for biolog

179 We show that in axolotl primitive ectoderm (animal caps; ACs) NANOG and

180 We have sequenced the axolotl Prod 1 promoter and selected two candidate sites

181 The epidermis overlying the migrating axolotl pronephric duct is known to participate in duct

182 in axolotls is diverse and precise, and that axolotls provide new insights about amphibian metamorpho

183 developed Accessory Limb Model (ALM) in the axolotl provides an opportunity to identify and characte

184 Notably, systemically activated axolotls regenerate limbs faster than naive animals, sug

185 gated mesoderm specification in embryos from axolotls, representing urodele amphibians, since urodele

186 f these mechanisms, genetic screening in the axolotl requires an extensive commitment of time and spa

187 opment, expression patterns of HoxD genes in axolotls resemble those in amniotes and anuran amphibian

188 We further show that Gli3 knockdown in axolotl results in a shift to postaxial dominant limb sk

189 en the tissues of neotenic and metamorphosed axolotls reveal the heterogeneity of non-immune parenchy

190 ent macromolecule synthesis was performed in axolotl salamander tissue using whole-mount click chemis

191 xpressed in its current configuration in the axolotl salamander.

192 Contrastingly, axonal projections of the axolotl (salamander) branch extensively before entering

193 We here show that the retina of axolotl salamanders contains at least two distinct class

194 supernumerary limbs from blastemal tissue in axolotl salamanders.

195 The axolotl serves as a primary model for studying successfu

196 haracteristics in neotenic and metamorphosed axolotls, serves as a resource to explore the molecular

197 egeneration stages shows that Polypterus and axolotl share a regeneration-specific genetic program.

198 RARE-EGFP reporter axolotls showed divergent reporter activity in limbs und

199 point of injury until reepithelialization in axolotl skin explant model and shown that cell layers mo

200 a, planarian, and salamander (i.e., newt and axolotl) species, but notably such regenerative capacity

201 ntified high levels of genome methylation in axolotl spermatozoa, with full-length transposons being

202 e showed that regenerating stem cells in the axolotl spinal cord revert to a molecular state resembli

203 reen fluorescent protein(+) transgenic white axolotl strains to map sites of hematopoiesis and develo

204 In addition, newt and axolotl Tbx4 and Tbx5 expression is regulated differentl

205 ially resolved single-cell transcriptomes of axolotl telencephalon sections during development and re

206 Analysis of axolotl testes and oocytes revealed diverse repertoires

207 ma growth, we generated a transgenic line of axolotls that ubiquitously expresses a bicistronic versi

208 l model for the regeneration of a CSD in the axolotl (the Excisional Regeneration Model) that allows

209 fter tail amputation in Ambystoma mexicanum (Axolotl) the correct number and spacing of dorsal root g

210 of the same structure we have turned to the axolotl, the master of vertebrate regeneration, and gene

211 e that, in two urodele amphibians, newts and axolotls, the regulation of Tbx4 and Tbx5 differs from h

212 kine/chemokine signaling are retained in the axolotl, they are more dynamically deployed, with simult

213 g transplant experiments with GFP-expressing axolotl, they show vividly which cells of the blastema r

214 d limb regeneration and tissue repair in the axolotl to be investigated in increasing detail, the mol

215 s create a hypersensitive kinase that allows axolotls to maintain this pathway in a highly labile sta

216 tion-incompetent retroviruses can be used in axolotls to permanently express markers or genetic eleme

217 hickness skin from ubiquitous GFP-expressing axolotls to wild-type hosts, we demonstrate that berylli

218 er salamander) and for A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map

219 This approach involved de novo assembly of axolotl transcripts, RNA-seq transcript quantification w

220 axolotls by a single i.p. injection and for axolotl transgenesis using I-SceI meganuclease and the m

221 e rearing water of the postembryonic Mexican axolotl was reinvestigated under conditions that permit

222 ory hair cells in lateral line neuromasts of axolotls was investigated via nearly continuous time-lap

223 In eya2 mutant axolotls, we found that DNA damage signaling through the

224 Axolotls, with their extensive abilities to regenerate a

225 t it is also able to promote regeneration in axolotl, Xenopus, and zebrafish.

226 ltered genomic composition of the laboratory axolotl, yielding a distinct, hybrid strain of ambystoma

227 oncordantly regulated across species (human, axolotl, zebrafish, and bichir)-including microRNA 21 (m