ライフサイエンスコーパス: axotomy

1 of precision techniques for severing axons (axotomy).

2 r the level of macrophage infiltration after axotomy.

3 egulated sodium channel expression following axotomy.

4 y improve the chances of axon regrowth after axotomy.

5 mulation was reduced but not abolished after axotomy.

6 model of partial retinal ganglion cell (RGC) axotomy.

7 m mouse dorsal root ganglion axons following axotomy.

8 esses Wallerian degeneration for weeks after axotomy.

9 ganglion cell (RGC) axon regeneration after axotomy.

10 the ability of glia to respond to secondary axotomy.

11 n living rats for 4 weeks after intraretinal axotomy.

12 TRPV1 expression normally observed following axotomy.

13 ificant decreases even at 12 weeks following axotomy.

14 ganglia after sciatic nerve or dorsal column axotomy.

15 ection, dorsal root rhizotomy and peripheral axotomy.

16 hich suggests an increased susceptibility to axotomy.

17 FMNs undergoing significant cell death after axotomy.

18 dergo robust regenerative growth after laser axotomy.

19 for engulfing degenerating axons after acute axotomy.

20 , all mediate robust axonal protection after axotomy.

21 C survival was evaluated 7 and 14 days after axotomy.

22 als in live zebrafish larvae following laser axotomy.

23 te to the loss of axonal sprouting following axotomy.

24 al nerve bundles was the initial response to axotomy.

25 ed axon cell-autonomously after facial nerve axotomy.

26 to superficial "pain-specific" laminae after axotomy.

27 ificantly reduced by 80% at 7 days following axotomy.

28 ng patterns that resembled those produced by axotomy.

29 cell type, stage of animal, and position of axotomy.

30 legans motor neurons after femtosecond laser axotomy.

31 a way that resembles changes observed after axotomy.

32 oneurones died within 3 weeks after neonatal axotomy.

33 R) is readily detectable in such cells after axotomy.

34 re profoundly altered shortly after neonatal axotomy.

35 sessed their capacity to protect axons after axotomy.

36 ush injury, chronic constriction injury, and axotomy.

37 ay contribute to elevated excitability after axotomy.

38 lular signaling molecule for RGC death after axotomy.

39 the survival of RGCs in tissue culture after axotomy.

40 ron excitability compared with the effect of axotomy.

41 a dissociated mixed retinal culture model of axotomy.

42 f RGC death by reactive oxygen species after axotomy.

43 dult rat, and the adult rat after peripheral axotomy.

44 ect RGCs in a rat optic nerve crush model of axotomy.

45 bility that were statistically equivalent to axotomy.

46 d these with the effects of peripheral nerve axotomy.

47 s associated with increased retraction after axotomy.

48 yelinating Schwann cells after sciatic nerve axotomy.

49 magnocellular neuronal (MCN) survival after axotomy.

50 y of RDLN motoneurons during aging and after axotomy.

51 distinct neuromuscular synaptic responses to axotomy.

52 nd p21/waf, that are coinduced in neurons by axotomy.

53 s retraction and enhanced regeneration after axotomy.

54 fferently than the acute, one time insult of axotomy.

55 ed in the facial nerve of CD38 KO mice after axotomy.

56 olecularly distinct subsets of mouse RGCs to axotomy.

57 glia and motoneurons changed with time after axotomy.

58 anabolic metabolism and is downregulated by axotomy.

59 ression changes that mirror those induced by axotomy.

60 taining neuronal circuitry following distant axotomy.

61 APK and Neu3 sialidase were not activated by axotomy.

62 the elevated axonal Ca(2+) that occurs after axotomy.

63 robust regenerative regrowth following laser axotomy.

64 choroidal blood flow was not affected by the axotomy.

65 port, and redox state before and after laser axotomy.

66 Within 4-6 days of axotomy 30-50 % of regenerated nerve terminals still occ

67 are differentially affected by sciatic nerve axotomy, a model of neuropathic pain in which degenerati

68 ction proteins (Cnga3, Gnao1) or by surgical axotomy abolished avoidance behaviors and/or cellular Ca

69 Axotomy activates an intrinsic pro-degenerative axon dea

70 In PNS axons, axotomy activates Neu3 sialidase, increasing the ratio o

71 lengthy retraction from the initial site of axotomy after spinal cord injury.

72 At 7 day following bilateral SCG axotomy, all targets were significantly depleted of TH i

73 Axotomy alone resulted in a prolonged change in regulati

74 rease in the size of motoneurons 7 days post-axotomy and a partial spontaneous recovery occurred by 2

75 n end that are sustained for hours following axotomy and completely eliminated within unc-68/RyR muta

76 erve regeneration in animal models following axotomy and crush injury.

77 eactive to many kinds of injuries, including axotomy and deafferentation.

78 The fact that this range collapses after axotomy and does not recover completely until after rein

79 These changes were associated with axotomy and explant and not with the initiation or progr

80 for the accelerated regeneration seen after axotomy and for the improved regeneration seen after a c

81 as rapidly upregulated in optic nerves after axotomy and in an optic nerve explant culture model.

82 uired for efficient activation of glia after axotomy and internalization/degradation of axonal debris

83 by which the distal nerve degenerates after axotomy and is cleared by phagocytosis.

84 rotection of septal cholinergic neurons from axotomy and led to similar levels of NGF accumulation in

85 hat potently block AxD in cellular models of axotomy and neuropathy.

86 s expression of glial engulfment genes after axotomy and reduces the ability of glia to respond to se

87 the time of nerve injury, many RGCs survive axotomy and regenerate their axons into the distal optic

88 ophic factor levels were also enhanced after axotomy and regeneration.

89 We have examined the effect of axotomy and systemic NT-3 administration on neuronal los

90 lling is activated in response to peripheral axotomy and that PI3K pathway is required for sensory ax

91 gulated in primary sensory neurons following axotomy and that this change occurs in larger neurons th

92 ificantly reduced from local responses after axotomy and that were tetrodotoxin (TTX)-sensitive in a

93 We used laser axotomy and time-lapse confocal imaging to characterize

94 n axonal degeneration with single-axon laser axotomy and time-lapse imaging, monitoring the initial c

95 e nerves degenerated at a similar rate after axotomy and to a similar extent in the experimental neur

96 s, in conjunction with previous studies with axotomy and traumatic brain injury, establish SARM1 as t

97 t properly infiltrate neuropil regions after axotomy and, as a consequence, fail to clear degeneratin

98 owing left sciatic nerve distal transection (axotomy) and treatment with GM1 ganglioside.

99 ensheathing glia acting as phagocytes after axotomy, and astrocytes potentially modulating synapse f

100 itability that develops in these cells after axotomy, and blocking the activation of apJNK in vitro d

101 ular localization of the EFF-1 fusogen after axotomy, and establishing phosphatidylserine (PS) and th

102 inal ganglion cells (RGCs) survive following axotomy, and even fewer regenerate axons.

103 in cluster size, beginning immediately after axotomy, and reaching maximum within one week.

104 ons of 29D7 were performed after optic nerve axotomy, and subsequent RGC survival was quantified usin

105 Edge-TREK2 decreased in C-neurons 7 d after axotomy, and their Ems depolarized by approximately 10 m

106 re medium to 25 mM rescued the MCNs from the axotomy- and TTX-induced cell death.

107 that deterioration of axons and myelin after axotomy are mechanistically distinct processes.

108 This validates the use of axotomy as an investigative tool in understanding the ro

109 cord after unilateral L(2)-L(4) dorsal root axotomy at multiple time points (from 16 h to 30 d after

110 lt hamster facial motoneurons (FMNs) survive axotomy at the stylomastoid foramen (SMF), whereas, befo

111 uscle during the first week after a neonatal axotomy, at a time when the motoneurones would be either

112 ngle axonal branches were dissected by laser axotomy, avoiding collateral damage to the adjacent dend

113 Freund's complete adjuvant) or nerve injury (axotomy; AXO, partial sciatic nerve ligation; PSNL, spin

114 After axotomy, bone marrow (Y-->X) chimeric rats were injected

115 > 7 months, axons were still protected from axotomy but synapses degenerated rapidly, in wild-type f

116 ons remain in a regenerative state following axotomy but the conditions provided by the I-PNG appear

117 All I(K(Ca)) subtypes are decreased by axotomy, but iberiotoxin-sensitive and clotrimazole-sens

118 omplex remains active for long periods after axotomy, but its activity diminishes during target conta

119 P--render the neuron less able to respond to axotomy by a rapid, forward, actin-dependent movement.

120 Previous studies have shown that neonatal axotomy causes massive motoneurone death and abnormal fu

121 parate and injure distal axons, we show that axotomy causes retrograde dendritic spine loss at direct

122 We explored how axotomy changes the physiology of severed axons and adja

123 can delay axonal degeneration initiated via axotomy, chemotherapeutic agents, or genetic mutations.

124 Since we have previously shown that axotomy decreases I(Ca) in DRG neurons, we investigated

125 Axotomy decreases I(K(Ca)) due to a direct effect on K((

126 Painful axotomy decreases K(ATP) channel current (IK(ATP)) in pr

127 Inducing multiple waves with additional axotomies did not change the kinetics of degeneration.

128 Axotomy did not reduce further the size of aged motoneur

129 Three days after axotomy, DTI revealed a lesion in the ipsilateral dorsal

130 Within the first 7 d after axotomy, E-cadherin is rapidly and completely lost from

131 Moreover, several hours post axotomy, early hallmarks of Wallerian degeneration (WD)

132 Axotomy elevated lipin1 in retinal ganglion cells, which

133 to regenerate and subsequently die following axotomy, even though there are high levels of CNTF in th

134 factor for forthcoming neuronal death after axotomy, expanding its implications beyond the neurodege

135 iopsies to create an intracutaneous excision axotomy followed by a concentric 4-mm overlapping biopsy

136 n, is needed for growth cone formation after axotomy, for generating a retrogradely transported injur

137 Axotomy had no effect on the number of RDLN motoneurons

138 rimental glaucoma, hemiretinal endodiathermy axotomy (HEA) of the RGCs produces no apparent anatomic,

139 egeneration in mouse sensory axons following axotomy, illustrating conservation of function.

140 uch as Pdcd2 in a subset of NP neurons after axotomy, implicating their actions in neuronal cell deat

141 lular debris, we performed femtosecond laser axotomies in genetic backgrounds lacking cell death gene

142 essential for axon regeneration after laser axotomy in adult neurons, and axotomy induces translatio

143 Ca(2+) response induced by Ca(2+) influx or axotomy in Bcl-2-expressing neurons than in control neur

144 We performed a facial nerve transection axotomy in both mSOD1 subgroups prior to disease onset a

145 lustrate the use of this setup by presenting axotomy in Caenorhabditis elegans as an example.

146 the distal portion of the facial nerve after axotomy in mice.

147 cally protects axons from degeneration after axotomy in mice.

148 cell somata are markedly more vulnerable to axotomy in neonatal mice.

149 is required to drive axon degeneration after axotomy in sympathetic and sensory neurons cultured in m

150 t, but to be upregulated after sciatic nerve axotomy in the dorsal root ganglia.

151 recently been proposed for femtosecond laser axotomy in the nematode C. elegans for immobilization of

152 Here we use femtosecond laser surgery for axotomy in the roundworm Caenorhabditis elegans and show

153 dria-localized protein, is upregulated after axotomy in this high regeneration condition.

154 neuron death after transection of the axons (axotomy) in neonates is believed to share the same mecha

155 Facial nerve axotomy increased the total cofilin abundance and also n

156 After axotomy, increased NKCC1 phosphorylation has been report

157 In young adult Wld(s) mice, axotomy induced progressive, asynchronous synapse withdr

158 ost animals showed a complete restoration of axotomy-induced alterations in eye position sensitivity,

159 ating neurons also undergo a delayed form of axotomy-induced apoptosis.

160 ctors reflected in significant reductions in axotomy-induced atrophy of large pyramidal neurons withi

161 eased mitochondrial motility, suppression of axotomy-induced Ca(2+) elevation in axons, and thereby s

162 usceptibility of presymptomatic SOD1 FMNs to axotomy-induced cell death and, by extrapolation, diseas

163 e effects of both androgens and estrogens on axotomy-induced cell death in one system and, with our p

164 adal steroids encompass neuroprotection from axotomy-induced cell death is the focus of the present s

165 ouse facial MN (FMN) are more susceptible to axotomy-induced cell death than wild-type (WT) FMN, whic

166 ble to rescue approximately 20% of FMNs from axotomy-induced cell death, with the effects permanent.

167 5 (P15), developing FMNs undergo substantial axotomy-induced cell death.

168 MKK4/MAP2K4-dependent mechanisms underlying axotomy-induced death are motoneuron autonomous.

169 ating a synergistic effect of MKK7/MAP2K7 on axotomy-induced death of these facial motoneurons.

170 ficient facial motoneurons were resistant to axotomy-induced death, indicating a synergistic effect o

171 hose mutant mice are completely resistant to axotomy-induced death.

172 t inhibition of GFRalpha3 did not affect the axotomy-induced decrease in CPM threshold, but transient

173 that inhibition of TRPV1 did not affect the axotomy-induced decrease in polymodal C-fiber (CPM) heat

174 TNFR1 death receptor pathway is involved in axotomy-induced FMN death in WT and is partially respons

175 gnificant differences were revealed when the axotomy-induced gene expression response of presymptomat

176 Axotomy-induced hyper-excitability of cultured neurons c

177 axonal degeneration in culture by preventing axotomy-induced local energy deficit and preserving mito

178 Axotomy-induced loss of I(Ca) may further potentiate cur

179 K/DLK and MKK4/MAP2K4-dependent mechanism in axotomy-induced motoneuron death in neonates and also de

180 death in neonates and also demonstrates that axotomy-induced motoneuron death is not identical to dev

181 nt animal models of neuronal injury, such as axotomy-induced neuronal death, neurotoxin-induced neuro

182 Axotomy-induced retinal ganglion cell (RGC) death in mam

183 activated in RGCs soon after injury and that axotomy-induced RGC death is attenuated in p53 heterozyg

184 hecally or to the proximal stump can prevent axotomy-induced sensory neuron loss and that NT-3 can st

185 Axotomy induces axonal Luman synthesis and also release

186 of the majority of RGCs, we demonstrate that axotomy induces differential activation of distinct path

187 Here we show that in Caenorhabditis elegans, axotomy induces ectopic expression of serotonin (5-HT) i

188 on after laser axotomy in adult neurons, and axotomy induces translation of CEBP-1 in axons.

189 The axotomy-inducible enzyme Nna1 defines a subfamily of M14

190 le processes including primary and secondary axotomy, inflammation, and glial scar formation that hav

191 ervation suggests that receptor blockade and axotomy interrupt the same signalling mechanisms but lea

192 Thus, activation of Smad1 by axotomy is a key component of the transcriptional switch

193 phagocytosis of axons following dorsal root axotomy is impaired in mice in which TRIF has been genet

194 The response of myelinating Schwann cells to axotomy is inhibited by PKI166 in vivo.

195 g of retinal ganglion cell (RGC) death after axotomy is partly dependent on the generation of reactiv

196 the mSOD1 molecular response to facial nerve axotomy is phenotypically regenerative and indistinguish

197 rvival of motor neuron cell somata following axotomy is well documented, but it remains unclear wheth

198 Its activation, after axotomy, is mediated by Aplysia c-Jun-N-terminal kinase

199 after injections of botulinum toxin inhibits axotomy-like changes in motoneurons.

200 de of endplate currents underlies the robust axotomy-like effects of alpha-bungarotoxin on motoneuron

201 ntrol animals and animals that had undergone axotomy, likely due to partial loss of excitatory inputs

202 These findings suggest that peripheral axotomy may quickly induce hyperexcitability of uninjure

203 ved neurotrophic factor following peripheral axotomy, might retune DRG neurons and contribute to thei

204 investigation, we utilized the facial nerve axotomy model and a presymptomatic amyotrophic lateral s

205 of labeled axons demonstrated that the laser axotomy model did not affect adjacent axon function.

206 ) nerve stumps on day 1 in the sciatic nerve axotomy model in rats.

207 er axotomy, we superimposed the facial nerve axotomy model on presymptomatic mSOD1 mice and investiga

208 Using an in-vitro laser axotomy model, we show a progressive decline in the abil

209 In excitotoxicity and axotomy models retinal ganglion cell death has been show

210 cell and axonal regeneration using validated axotomy models to study and compare patterns and the rel

211 show in both neurotoxin and physical injury (axotomy) models that Myr-Akt is also able to preserve do

212 Following facial nerve axotomy, nerve function is not fully restored even after

213 These data support the proposal that after axotomy, neural activity is neuroprotective in the HNS.

214 After peripheral axotomy, neuronal Smad1 is upregulated, and phosphorylat

215 After axotomy, neuronal survival and growth cone re-formation

216 We showed that axotomy of both PLMs leads to a dramatic loss of posteri

217 sion enables full recovery of function after axotomy of Caenorhabditis elegans mechanosensory neurons

218 contributing to synaptic transmission after axotomy of DRG neurons.

219 Here we report that mechanical axotomy of Drosophila neurons in culture triggers axonal

220 We report that axotomy of giant RS neurons induces a select subset of t

221 Axotomy of live wild-type axons induced a dramatic spike

222 Axotomy of peripheral nerve stimulates events in multipl

223 Axotomy of peripheral nerve triggers events that coordin

224 Here we show that axotomy of PLM sensory neurons triggers axonal calcium w

225 n expression was also examined after central axotomy of rubrospinal neurons, which constitutively sho

226 Using laser axotomy of single neurons in Caenorhabditis elegans, we

227 This method allows for the axotomy of specific subsets of axons followed by examina

228 ells) in dorsal root ganglia (DRG) following axotomy of the adult rat sciatic nerve.

229 re quantified in adult rats after unilateral axotomy of the chorda tympani nerve and/or maintenance o

230 We show that following axotomy of the distal axons, inhibition of Na(V)1.7 and

231 Axotomy of the peripheral branch of adult dorsal root ga

232 nervation of most vascular targets following axotomy of the SCG with only minimal recovery of TH prot

233 as non-vascular targets following bilateral axotomy of the superior cervical ganglion (SCG) at short

234 for target-derived trophic factors, because axotomy of these neurons in adulthood results in minimal

235 To investigate the effect of axotomy on synaptic transmission between dorsal root gan

236 y, we have examined the effect of peripheral axotomy on VGLUT1 expression in central terminals of mye

237 onal mitochondria were rapidly depleted upon axotomy or downregulation of Nmnat.

238 used by withdrawal of other trophic support, axotomy or vincristine exposure.

239 ablation blocks axon degeneration induced by axotomy or vincristine treatment, while SARM acts in par

240 emoved surgically at the same time as distal axotomy, or at a later time.

241 gulation of all three receptors in following axotomy, possibly in response to changes in growth facto

242 erve regeneration studies, including on-chip axotomy, post-surgery housing for recovery, and post-rec

243 acilitates human RGC axon regeneration after axotomy, providing evidence that the recapitulation of d

244 In marked contrast, axotomy rapidly transformed damaged neurons into just tw

245 nhance axonal regeneration and indicate that axotomy rather than diffusible factors released at the i

246 Axotomy regeneration was repeated in month 4 during this

247 ndent G3BP1 phosphorylation on Ser 149 after axotomy releases axonal mRNAs for translation.

248 in only the RGCs, without inducing either an axotomy response or axon degeneration.

249 This is supported by the fact that axotomy results in increased CGRP production in the nerv

250 Similarly, axotomy results in increased NKCC1 phosphorylation in do

251 gated how the axonal mRNA pool changes after axotomy, revealing that numerous gene transcripts relate

252 ctions in the temporal retina (distal to the axotomy) showed loss of GC soma and NFL degeneration, wh

253 of phosphatidylserine externalization after axotomy.SIGNIFICANCE STATEMENT Axonal degeneration is a

254 H with electrophysiological properties after axotomy similar to those of mammalian neurons and are an

255 ynamic pro-survival/regenerative response to axotomy, similar to WT, despite increased cell death.

256 emonstrated sparing of adjacent axons to the axotomy site.

257 rapid reinnervation of the epidermis at the axotomy site.

258 N methylpyridinium iodide (4Di-10ASP) at the axotomy site.

259 ntagonist, receptor-associated protein, into axotomy sites in adult rats.

260 ups of rats: (1) untreated; (2) spinal nerve axotomy (SNA), L5 SNA 1 week earlier; (3) mSNA (modified

261 gs help to explain why CNS neurons die after axotomy, strongly suggest that A1 astrocytes contribute

262 skin at various times after saphenous nerve axotomy suggested multiple changes in neurotrophic facto

263 We used this capsaicin axotomy technique to examine the effects of exercise on

264 EE) followed by a conditioning sciatic nerve axotomy that precedes a spinal cord injury (SCI).

265 as a gene induced in motor neurons following axotomy the finding that its loss leads to selective neu

266 Following a 7 day axotomy, the 22-24 kDa NGF species and the mature 14 kDa

267 Following axotomy there was segmentation of axons in the epidermis

268 After sciatic nerve axotomy, there was a rightward shift in the cell-size di

269 s demonstrates that mitochondria shrink post-axotomy, though analysis of complex IV activity suggests

270 Subjects also underwent a chemical 'axotomy' through the topical application of capsaicin, a

271 Using laser axotomy to induce Wallerian degeneration (WD) in zebrafi

272 protein species present in the SCG following axotomy (transection) or injury of the post-ganglionic a

273 First, axotomy triggered a transient local calcium wave origina

274 dinal DCI in rats that underwent dorsal root axotomy triggering Wallerian degeneration of axons-a pat

275 Peripheral axotomy triggers transport of the beta-actin 3'-UTR cont

276 After subcortical axotomy, trkB transduction induced corticospinal axon re

277 Axotomy typically leads to retrograde neuronal degenerat

278 ltiple neuronal types can regrow after laser axotomy using a variety of lasers.

279 eptor pathways after target disconnection by axotomy vs. disease progression.

280 we report that macrophage accumulation after axotomy was abolished in both the dorsal root ganglion (

281 Intraretinal axotomy was achieved by multiple treatments with a diode

282 found a significant increase, the effect of axotomy was determined on superoxide levels independent

283 No change in the GM1/GD1a ratio after axotomy was observed in retinal axons (in vitro and in v

284 c-to-nucleus translocation of P-cofilin upon axotomy was reduced in motoneurons expressing SRF-DeltaN

285 of cell death, long associated with proximal axotomy, was seen via TUNEL or routine light microscopy/

286 ngly induced in DRG neurons after peripheral axotomy, we crossed the ZW-X mouse line with a mouse tha

287 After peripheral nerve axotomy, we found that LTMR afferents with narrow, uninf

288 underlying the enhanced mSOD1 FMN loss after axotomy, we superimposed the facial nerve axotomy model

289 infected and uninfected macaques, excisional axotomies were performed in nonglabrous skin at 14-day i

290 cutaneous regeneration after thigh capsaicin axotomy were compared for participants with type 2 diabe

291 from cholinergic dedifferentiation following axotomy when administered in vivo.

292 fragments containing a mitochondrion survive axotomy, whereas those lacking mitochondria degenerate.

293 the vulnerability of synaptic boutons after axotomy, which depend on cell-type and their recent hist

294 localized increase in caspase activity upon axotomy, which is absent in ced-4 and ced-3 loss-of-func

295 al scanning laser ophthalmoscope and in vivo axotomy with a diode-pumped solid-state laser to assess

296 A group of Lewis rats underwent optic nerve axotomy with the application of N-4-(4-didecylaminostyry

297 We combined single-cell laser axotomy with time-lapse imaging to study the dynamics of

298 strongly and rapidly downregulated following axotomy, with a time course that matches the development

299 d by whisker stimulation, potentially due to axotomy within and surrounding the whisker circuit as vi

300 synthesis for axonal growth and responses to axotomy, yet there is little insight into the functions