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1 perimentally tractable system, lysis time in bacteriophage T7.
2 polymerase (pol), the replicative enzyme for bacteriophage T7.
3 process that is similar to one proposed for bacteriophage T7.
4 also been found for Escherichia coli but not bacteriophage T7.
5 olymorphism data over time for four genes in bacteriophage T7.
6 mputer model for the intracellular growth of bacteriophage T7.
7 cturally simple RNA polymerase is encoded by bacteriophage T7.
8 DNA junction-selective resolving enzyme from bacteriophage T7.
9 tions among the proteins of Escherichia coli bacteriophage T7.
10 olecular bases of the packaging mechanism of bacteriophage T7.
11 the only known nucleotide kinase encoded by bacteriophage T7.
12 mple is the infection of Escherichia coli by bacteriophage T7.
13 date (VV) catalyzed by the DNA polymerase of bacteriophage T7.
14 d DNA synthesis mediated by the replisome of bacteriophage T7.
15 in a single polypeptide encoded by gene 4 of bacteriophage T7.
16 rpreting experimental evolution studies with bacteriophage T7.
17 ining the type I restriction enzyme EcoKI by bacteriophage T7 0.3 mutants leads to restriction during
22 in Ocr (overcome classical restriction) from bacteriophage T7 acts as a mimic of DNA and inhibits all
23 n addition, the gene 10 leader sequence from bacteriophage T7 and a minicistron localized upstream of
25 resembles the single polypeptide RNAPs from bacteriophage T7 and T3, requires a separate specificity
26 rized here include family A polymerases from bacteriophage T7 and Thermus aquaticus, family B polymer
27 s of a multiprotein replication complex from bacteriophage T7 and to characterize the effect of prima
28 lar crowding using the replication system of bacteriophage T7 and we show that it affects several asp
32 pproximately 650-kDa functional replisome of bacteriophage T7 assembled on DNA resembling a replicati
34 The traE gene product has been cloned into a bacteriophage T7-based transient expression system, and
38 coli RNA polymerase and the A1 promoter from bacteriophage T7 by monitoring alterations in the intrin
39 that the gene 4 primase/helicase encoded by bacteriophage T7 can form both hexamers and heptamers.
40 nit DNA-dependent RNA polymerase (RNAP) from bacteriophage T7 catalyzes both promoter-dependent trans
41 cture of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a
46 ase domain of the gene 4 helicase-primase of bacteriophage T7 contains a zinc motif and a catalytic c
48 he replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that
49 ent studies in the model RNA polymerase from bacteriophage T7 demonstrate that upstream duplex contac
50 ent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these r
55 We present the first structural model of a bacteriophage T7 DNA helicase-DNA polymerase complex usi
59 system for replicative DNA polymerases, the bacteriophage T7 DNA polymerase (gp5), encoded by gene 5
61 ctionally important conformational change in bacteriophage T7 DNA polymerase (T7 pol) that use the en
62 plicative polymerases, exonuclease-deficient bacteriophage T7 DNA polymerase (T7(-)) and HIV reverse
63 rol, to observe DNA replication catalysed by bacteriophage T7 DNA polymerase (T7DNAP) and by the Klen
66 opposite A following all three base pairs by bacteriophage T7 DNA polymerase exo- showed burst kineti
70 ructures of the pol gamma-beta dimer and the bacteriophage T7 DNA polymerase ternary complex, which s
71 te the (+)-trans-anti-[BP]-N(2)-dG adduct by bacteriophage T7 DNA polymerase with the order of prefer
78 tion kinetics of the human mitochondrial and bacteriophage T7 DNA polymerases on free-ssDNA, in compa
79 is study, we visualize fluorescently labeled bacteriophage T7 DNA polymerases within the replisome wh
82 than model replicative polymerases, such as bacteriophage T7(-) DNA polymerase and human immunodefic
83 aging, and molecular dynamics simulations on bacteriophage T7 DNAp and SSB, we investigated molecular
96 n interaction between the K296P mutant and a bacteriophage T7-epitope-tagged K64E mutant of dsRNA bin
97 V-1 RT reverse transcriptase (HIV-1 RT), and bacteriophage T7 exo- (T7(-)) were examined to determine
104 scherichia coli that survive coexpression of bacteriophage T7 gene 10 and plasmid F pifA has allowed
108 um nucleotide binding and oligomerization of bacteriophage T7 gene 4 helicases have been investigated
110 base stack of other helicases, including the bacteriophage T7 gene 4 protein and bacterial DnaB helic
112 t exhibits strong sequence homology with the bacteriophage T7 gene 4 protein primase-helicase (T7 gp4
116 ed mutations have altered the composition of bacteriophage T7 genome and suggest that this may be a s
117 of cellular internalization of an infecting bacteriophage T7 genome has led to surprising observatio
118 In a normal infection about 850 bp of the bacteriophage T7 genome is ejected into the cell, the re
120 ate residue for transglycosylase activity of bacteriophage T7 gp16 is not essential for phage growth,
121 nd suggest, by comparison with the hexameric bacteriophage T7 gp4 and SV40 large T-antigen helicases,
124 multifunctional protein encoded by gene 4 of bacteriophage T7 (gp4) provides both helicase and primas
128 hin the hexameric gene 4 helicase-primase of bacteriophage T7 has been examined by using two genetica
129 he DNA polymerase encoded by gene 5 (gp5) of bacteriophage T7 has low processivity, dissociating afte
135 acterized nine helicase-deficient mutants of bacteriophage T7 helicase-primase protein (4A') prepared
137 a single-subunit RNA polymerase (RNAP) from bacteriophage T7, implementing all-atom molecular dynami
138 genome reveals that it is closely related to bacteriophage T7 in both genome organization and sequenc
139 rapid size and titre analysis of unlabelled bacteriophage T7 in both salt solution and mouse blood p
140 e of the DNA polymerase encoded by gene 5 of bacteriophage T7, in a complex with its processivity fac
143 rary was prepared from RNA synthesized after bacteriophage T7 infection and the sequence of bacteriop
144 o plays a role in protecting E. coli against bacteriophage T7 infection by limiting the dGTP required
145 talytic activity is known to increase during bacteriophage T7 infection, reflecting the expression of
151 (overcome classical restriction) protein of bacteriophage T7 is a molecular mimic of double-stranded
157 The single-subunit RNA polymerase (RNAP) of bacteriophage T7 is able to perform all steps of transcr
158 n extracts of Escherichia coli infected with bacteriophage T7 is able to repair double-strand breaks
161 A synthesis in the DNA replication system of bacteriophage T7 is catalyzed by the primase domain of t
162 ngle-stranded DNA (ssDNA) binding protein of bacteriophage T7 is essential for T7 DNA replication and
165 The ocr protein, the product of gene 0.3 of bacteriophage T7, is a structural mimic of the phosphate
175 ding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replica
177 expression levels in 6,348 experiments using bacteriophage T7 polymerase to synthesize messenger RNA
180 ment that the mitochondrial DNA helicase and bacteriophage T7 primase-helicase share physical feature
183 ilayer structure of the portal vertex of the bacteriophage T7 procapsid, the recipient of T7 DNA in p
184 ces for COR15am and COR6.6 were fused to the bacteriophage T7 promoter and expressed in Escherichia c
186 FLP recombinase gene under the control of a bacteriophage T7 promoter, (ii) it confers kanamycin res
187 ne) of Escherichia coli deformylase behind a bacteriophage T7 promoter, we have, however, been able t
188 cloned under control of the strong inducible bacteriophage T7 promoter, yet induction did not yield d
193 rcular and linear M13 double-stranded DNA by bacteriophage T7 proteins have been examined by electron
200 ligate three-component system comprising the bacteriophage T7-related mitochondrial RNA polymerase, t
202 we have demonstrated that the passage of the bacteriophage T7 replication complex is blocked in vitro
205 proach to visualize this coordination in the bacteriophage T7 replisome by simultaneously monitoring
206 nd ensemble methods, we demonstrate that the bacteriophage T7 replisome is able to directly replicate
208 nation-dependent replication promoted by the bacteriophage T7 replisome was disrupted by substitution
209 tructure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two
210 on-denaturing agarose gel electrophoresis of bacteriophage T7 reveals two states of the mature T7 cap
212 common reaction conditions and components on bacteriophage T7 RNA polymerase (RNAP) activity using a
216 elongation complex (EC), the single-subunit bacteriophage T7 RNA polymerase (RNAP) undergoes dramati
217 (2)-epsilon G acts as a partial block to the bacteriophage T7 RNA polymerase (RNAP), which allows nuc
221 ns, IVT of mRNA is typically performed using bacteriophage T7 RNA polymerase (T7 RNAP) owing to its a
225 scription by Gal4, (ii) transcription by the bacteriophage T7 RNA polymerase (T7RNAP), and (iii) FLP-
226 binding kinetics between single molecules of bacteriophage T7 RNA polymerase and DNA, as a function o
227 ecifically modified O(6)-meG DNA template by bacteriophage T7 RNA polymerase and human RNA polymerase
229 developed to control the expression of both bacteriophage T7 RNA polymerase and recombinant gene(s)
230 d a copy of the ORF under the control of the bacteriophage T7 RNA polymerase and the Escherichia coli
231 T7) which constitutively express cytoplasmic bacteriophage T7 RNA polymerase and which are derived fr
232 dvantage of an experimental system that uses bacteriophage T7 RNA polymerase as a probe for aspects o
235 and 'non-replicating' vectors, encoding the bacteriophage T7 RNA polymerase for transcription of rec
236 uced nucleosome sliding and transcription by bacteriophage T7 RNA polymerase from the nucleosomal tem
237 ed, which carries a chromosomally integrated bacteriophage T7 RNA polymerase gene expressed from a la
238 a recombinant vaccinia virus expressing the bacteriophage T7 RNA polymerase in the presence of the D
244 uctures of transcription complexes formed by bacteriophage T7 RNA polymerase reveal a nucleotide-addi
245 the initiation of transcription in the model bacteriophage T7 RNA polymerase system, the simplest pos
248 omoter binding and open complex formation in bacteriophage T7 RNA polymerase was investigated using 2
249 tic mechanism of transcription initiation by bacteriophage T7 RNA polymerase was investigated using t
250 yeast transcriptional activator GAL4 and for bacteriophage T7 RNA polymerase were inserted into the b
254 sion of cell death by the combination of the bacteriophage T7 RNA polymerase-lysozyme system and an i
259 ined evidence that open complex formation in bacteriophage T7 RNA polymerase:promoter binary complexe
260 ced in vitro-transcribed mRNAs using several bacteriophage T7 RNA polymerases, including one wild-typ
261 ssay to quantify transcription elongation by bacteriophage T7 RNAP on small, circular DNA templates a
264 ed as well as the steady-state population of bacteriophage T7-specific transcripts were examined for
266 eplication loops by individual replisomes of bacteriophage T7 supporting coordinated DNA replication.
267 in minutes after infecting Escherichia coli, bacteriophage T7 synthesizes many copies of its genomic
270 single-polypeptide-chain RNA polymerase from bacteriophage T7 (T7RNAP), like other RNA polymerases, u
272 tal structure of TTPA resembles those of the bacteriophage T7 tail protein gp11 and gp4 of bacterioph
273 a simulation based on experimental data from bacteriophage T7 that computes the developmental cycle o
275 ndonuclease I is a 149 amino acid protein of bacteriophage T7 that is a Holliday junction-resolving e
276 fficiency of a replicative DNA helicase from bacteriophage T7 that is a member of a class of helicase
277 I is a junction-resolving enzyme encoded by bacteriophage T7, that selectively binds and cleaves fou
280 as a result of an internal protein unique to bacteriophage T7, the T7 genome may be significantly mor
281 h medium selectively inhibits the ability of bacteriophage T7 to infect Escherichia coli by inhibitin
283 overexpressed in Escherichia coli by using a bacteriophage T7 transient expression system, and a prot
285 han residues are dispersed in the primase of bacteriophage T7: Trp-42 in the ZBD and Trp-69, -97, -14
289 of the zinc-binding domain of DNA primase of bacteriophage T7 using a bacterial homolog from Geobacil
293 n extracts of Escherichia coli infected with bacteriophage T7 was used to monitor repair of double-st
294 n extracts of Escherichia coli infected with bacteriophage T7 was used to study the mechanism of doub
295 stem based on Escherichia coli infected with bacteriophage T7 was used to test for involvement of hos
296 es the protein of the head-tail connector of bacteriophage T7, was expressed in Escherichia coli.
297 d mutations have influenced the evolution of bacteriophage T7, we analyzed its genome for a bias in b
299 taining the scaffolding and head proteins of bacteriophage T7 were isolated after both proteins were
300 HaeII restriction fragments of the DNA from bacteriophage T7, which range in length from 474 to 6514