ライフサイエンスコーパス: basic residue

1 omains of the channel that contain conserved basic residues.

2 classical cleavage sites that do not contain basic residues.

3 ocation signal (NLS) mapped to 3 clusters of basic residues.

4 tions with DNA or RNA, mediated by conserved basic residues.

5 ed by N-terminal addition of MTDs containing basic residues.

6 ctivate protein precursors by cleavage after basic residues.

7 spectrometry revealed RCS adducts on select basic residues.

8 rsor activation through processing at paired basic residues.

9 dues and possibly with assistance from polar/basic residues.

10 These sites contain from 1 to 5 basic residues.

11 ly via the myristoyl moiety and remaining MA basic residues.

12 s does not depend on any specific cluster of basic residues.

13 ighly conserved surface that is dominated by basic residues.

14 ny partially occupied sites near clusters of basic residues.

15 like CD16A, whose TM domains do not contain basic residues.

16 mer includes 34 acidic residues and only six basic residues.

17 a hydrophobic center surrounded by conserved basic residues.

18 ween sulfonate groups and the side chains of basic residues.

19 acial binding site has no obvious cluster of basic residues.

20 e driving force for a tight binding of these basic residues.

21 A single basic residue above the si-face of the flavin ring is th

22 are resolved between the PP(i) mimic and two basic residues absolutely conserved in the fingers domai

23 stabilizing electrostatic interactions with basic residues across elements of secondary structure, f

24 MTDs containing basic residues alone fail to be targeted to the virologi

25 ther crystallin-derived peptides with double basic residues also lodged in the cell membrane fraction

26 t, in addition to the aromatic residues, the basic residues also play an important role in the format

27 independent innovations of 2-oxoacid-binding basic residues among these superfamilies.

28 thod to enrich phosphopeptides with multiple basic residues, an under-represented class in common enr

29 Absence of a conserved basic residue and presence of acidic residues in the ves

30 iological studies indicate that neutralizing basic residues and breaking the amphipathic helix dramat

31 rt, by the interactions between a cluster of basic residues and phosphatidic acid.

32 tent with the previous observation that both basic residues and phosphorylation control membrane resi

33 t, NepII offers advantageous cleavage to all basic residues and produces shortened peptides that coul

34 , RVB, and RVC NSP4s, but the arrangement of basic residues and the amphipathic alpha-helices was sub

35 ibosomes resides at the N-terminal conserved basic residues and the extended C-terminal segment, whic

36 ween the sidechains of these solvent-exposed basic residues and the graphene surface provide the driv

37 and (KA)(n) motifs and determined that both basic residues and the heparin-induced alpha-helix forma

38 raction is favored by the presence of nearby basic residues and the ITAM tyrosines.

39 e of Ty3 NC mutants show that the N-terminal basic residues and the unique zinc finger at the C-termi

40 ous proprotein convertase that cleaves after basic residues and transforms secretory proproteins into

41 We found that substituting all five basic residues, and seven UM-associated substitutions, i

42 cleave their substrates at single or paired basic residues, and SKI-1/S1P cleaves its substrates at

43 s are present in salt bridges with conserved basic residues, and that phosphorylation has the potenti

44 tion motif shared with GAP43 and surrounding basic residues are all required for efficient palmitoyla

45 Both aromatic and basic residues are critical for the protein anchoring on

46 Because basic residues are found between the loop Cys residues o

47 ence analysis on human GPCRs showed that the basic residues are frequent in the membrane-proximal reg

48 The toxin orients such that many basic residues are in the aqueous phase, all three Trp r

49 In periods P1, P4, and P6, basic residues are most important for actin affinity, in

50 When basic residues are mutated to alanine in the alpha4 subu

51 ontain zwitterionic sites if both acidic and basic residues are present and the overall charge densit

52 179)) located in the ECL2 of hNTS2 or with a basic residue (Arg(212)) at the same position in hNTS1.

53 Here we demonstrate that another pair of basic residues (Arg(310)-Arg(311)) in the membrane-proxi

54 For example, basic residues (Arg, His, Lys) increase peptide retentio

55 Mutation of only one of the two basic residues, Arg(277), correlates with loss of nuclea

56 Five basic residues (Arg32, Arg106, Arg409, Arg428, and Lys29

57 The AMPV-7 F protein has a single basic residue arginine (R) at position -1 in the F cleav

58 disease virus isolates and to contain 4 or 5 basic residues as well as isoleucine in the +2 position:

59 resented here suggests an important role for basic residues as well.

60 ena could be attributed to the presence of a basic residue at position 32.

61 horylate subsets of these motifs that have a basic residue at the +2 position and a proline residue a

62 Optimal Cdc14 substrates also possessed a basic residue at the +3 position relative to the phospho

63 s depend mainly on peptide charge (number of basic residues at acidic pH) and size.

64 rg(198) at P1 and a combination of two other basic residues at either P2 (Lys(197)), P6 (Arg(193)), o

65 Basic residues at position 427 prevented the Phe clamp f

66 This is further confirmed by mutations of basic residues at the anion-binding site which also demo

67 binding loops at the top of both domains and basic residues at the bottom of the C2B domain (direct b

68 However, mutation of three successive basic residues at the C terminus of the WH2-like sequenc

69 oI-CTD, a 27-amino-acid tail that is rich in basic residues at the C-terminal end is responsible for

70 Three basic residues at the C-terminal region play a critical

71 the export block was due to the presence of basic residues at the extreme N terminus of each enzyme.

72 ction with Abeta42 oligomers is a cluster of basic residues at the extreme N terminus of PrP (residue

73 ent the protonation of the conserved, buried basic residues at the intracellular entrance of the pore

74 Conserved basic residues at the N terminus of the channel are impo

75 geranylated C-terminus of Ggamma2 along with basic residues at the N-terminus of Galphai, and suggest

76 ary screening to determine that PASK prefers basic residues at the P-3 and P-5 positions in substrate

77 Substitution of basic residues at the predicted proprotein convertase cl

78 rved at the constriction zone, with multiple basic residues attached to the wall of the beta-barrel (

79 lar, tetrameric peptides featuring the Lewis-basic residue beta-dimethylaminoalanine (Dmaa) are capab

80 Mutation of four evolutionarily conserved basic residues blocks GSDMD-NT oligomerization, membrane

81 Several other conserved basic residues bordering the central basic patch and a s

82 rs at classical cleavage sites consisting of basic residues by well studied endopeptidases belonging

83 entral basic patch and a separate cluster of basic residues, called the first basic patch, were also

84 tation experiments show that both acidic and basic residues can catalyze the reaction.

85 targeting domains (MTDs) containing multiple basic residues can efficiently substitute MA to direct p

86 hat dense arrays of complementary acidic and basic residues can provide conformational stabilization

87 In the structure, we identify 2 basic residue clusters, one of which is important for ds

88 Loss of basic residues complementary to the acidic phosphosites

89 hese results show a variable contribution of basic residues comprising the APC exosite, with signific

90 Basic residues comprising the primary HS binding sites o

91 Basic residues contained in the 39-, 60-, and 70-80-loop

92 In addition, basic residues contributing to heparin binding and hepar

93 at KKRSHLKR(199) downward arrow (underlined basic residues critical for the recognition by PCs), but

94 Mutation of basic residues decreased heparin binding and activation

95 Moreover, replacement of the UL37 MTSbeta basic residues did not reduce OMM import.

96 Second, two basic residues distal to the palmitoylation site are req

97 th site-directed mutagenesis identified five basic residues distributed across the N terminus and the

98 eport that CAP3 mediates cleavage of ENaC at basic residues downstream of the furin site.

99 acting peptide (PIP) motifs, which both have basic residues downstream, function redundantly in Cdt1

100 d fragmentation: protons remain localized on basic residues during ETD but easily mobilize along the

101 A similar mutant with one additional basic residue, EGFR Mut R1-5, fails to exhibit ligand-in

102 Via 'phosphate steering', basic residues energetically steer an inverted ss 5'-fla

103 calation, our findings suggest that specific basic residues enhance these interactions, resulting in

104 d cardiolipin from extracellular fluid via a basic residue-enriched motif.

105 However, mutating a pair of conserved basic residues (equivalent to Arg-414 and Arg-415 of APL

106 This variant contains a mutation of a basic residue essential for proton transfer and solvent

107 eraction of TDG with PCNA or change critical basic residues essential for the action of the PIP degro

108 of bipartite NLS consisting of a triplet of basic residues followed by a tail of scattered basic ami

109 ton permeation despite the substitution of a basic residue for Glu(in).

110 FRET experiments revealed the importance of basic residues for the interaction between ISLAD CM form

111 ity filter, whereas the side chains of other basic residues form electrostatic complexes with two aci

112 Hence, interacting acidic and basic residues form favorable AspH(0)-H2O(0)-Arg(+) inte

113 Several kinds of evidence show that basic residues found on the distal N- and C-terminal dom

114 ned an N-terminal domain surface, comprising basic residues from both the N1 and N2 subdomains, that

115 esults highlight the importance of conserved basic residues from the EBOV VP35 C-terminal IID and val

116 protein's 2-fold axis, making contacts with basic residues from the second protomer.

117 inding: in the disordered state, a number of basic residues gain sufficient conformational freedom-la

118 arboxymethylation, along with a patch of HVR basic residues help foster membrane binding.

119 Mutagenesis of basic residues herein, followed by functional studies, i

120 Tyr536, which flanks the flavin ring, with a basic residue (histidine or arginine) did not significan

121 aid in recognition of a cluster of conserved basic residues hypothesized to facilitate trimer disasse

122 Simultaneous substitution of more than two basic residues impairs membrane targeting.

123 Myo1G Myo1PH domain reveals another critical basic residue in the beta3 strand, which is shared only

124 nteractions with the substrate adenosine and basic residues in alpha5 and the alpha5-alpha6 linker th

125 We conclude that C-terminal basic residues in cardiac TnT are critical for the regul

126 A cluster of basic residues in CBM77 confers calcium-independent reco

127 NC binds membranes via basic residues in either the distal or proximal zinc fin

128 When basic residues in homologous regions of the delta subuni

129 Mutation of two basic residues in HTLV-2 MA alpha-helix II, previously i

130 Reduction of mobility is associated with basic residues in its nucleocapsid (NC) domain, whereas

131 raA, involving interactions between specific basic residues in loop regions and phosphate oxygens of

132 An electrostatic interaction requires the basic residues in loops A and B, and gp2.5 binds to both

133 Basic residues in MA that are required for MLV Gag recru

134 Basic residues in MA that are required for the Gag local

135 3 interacts with voltage-VGCCs via a pair of basic residues in Munc13's C2B domain.

136 This result indicated that the basic residues in NC are important for virus budding.

137 The mutation of basic residues in NC caused a pronounced decrease in vir

138 The interface is comprised of basic residues in PSG and an acidic region in CC.

139 Basic residues in RPL11 are crucial for the stable bindi

140 r, this work implicates a potential role for basic residues in sequence-order specificity for peptide

141 Basic residues in site 1 and acidic residues in site 2 w

142 seems to correlate with a greater number of basic residues in the "foot" whereby a monotopic protein

143 association, because mutating either of two basic residues in the "signature motif" destroys membran

144 Several basic residues in the 1A domain which were previously pr

145 ough Akt activation involves PIP3 binding to basic residues in the Akt pleckstrin homology domain, aP

146 Previous studies of Nox2 showed that certain basic residues in the B-loop are important for activity

147 Mutation of basic residues in the binding sites resulted in the loss

148 ly, mutation of select hydrophobic or select basic residues in the Ca(2+)-binding loops reduces membr

149 demonstrate that Ser-505 phosphorylation and basic residues in the catalytic domain principally act t

150 Previous studies have implicated conserved basic residues in the cytosolic portions of beta- and ga

151 and SKI-1/S1P cleaves its substrates at non-basic residues in the Golgi.

152 to determine the roles played by all of the basic residues in the HBR.

153 their different topologies and the number of basic residues in the highly basic MA region.

154 in part, through Gbetagamma interaction with basic residues in the intracellular loop.

155 This latter effect was due to changes in basic residues in the linker region of PLN.

156 Here, we investigate the role played by basic residues in the N-terminal domain, the N-terminal

157 Mutagenesis of S4 in Kv7.1 indicates that basic residues in the N-terminal half (S4-N) and C-termi

158 Mutating each of the basic residues in the PBR to an alanine (Vam7-6A) led to

159 ing surface for RNA interacting at RRM4, via basic residues in the preceding linker.

160 he bilayer and (ii) with the total amount of basic residues in the protein.

161 the exogenous pseudosubstrate suggests that basic residues in the pseudosubstrate sequence are requi

162 riginates from interactions between multiple basic residues in the target peptide and the extensive n

163 omodimer, and RNA binding required conserved basic residues in this domain.

164 urating Ca(2+) Here, we investigated whether basic residues in this TnT region are involved in these

165 We demonstrate that equivalent basic residues in yeast Puf6 are important for RNA bindi

166 CE4, and PC7 cleave secretory proteins after basic residues, including the HIV envelope glycoprotein

167 Furthermore, neutralization of other H4c basic residues inhibited slc13 transport function, thus

168 e F2 subdomain to anionic lipids via several basic residues; insertion of an initially buried, conser

169 In contrast, in a micelle the basic residue interacts with only one of the acidic resi

170 ProTx2 positions two basic residues into the extracellular vestibule to antag

171 but accepting relatively few residues when a basic residue is anchored in pocket 6.

172 ing is intriguing because while an analogous basic residue is present in WbpE homologues that do not

173 ey ligate the cation, whereas the binding of basic residues is no longer favorable due to charge repu

174 a hydrophobic leader and downstream proximal basic residues is similar to that of the translocase of

175 peptides, having the same net charge and one basic residue, is affected by their specific orientation

176 ated the importance of the single additional basic residue (K) at position -3.

177 The results show that basic residues K125, R128, and R188 are important for te

178 etween a protonated H435 and its surrounding basic residues, K295, R396, and K438, at low pH.

179 Replacement of solution-exposed basic residues (K587, K589, R590, and R591) located on t

180 Removal of both conserved basic residues (K93A/R100A) was no more detrimental to r

181 07 stipulates that the additional N-terminal basic residue keeps S107 from assuming the topology of S

182 Somewhat surprisingly, basic residues like arginine, also played an equally or

183 ucture identifies surfaces rich in conserved basic residues likely vital for recognition of the RPR a

184 d' states of the motor show that a number of basic residues lining the nuclease groove are positioned

185 that removing the electric charges from key basic residues located between the DHFR and TS active si

186 Mutations of basic residues located in the NC domain of Gag that are

187 ng to whole cell walls, which is mediated by basic residues located on other D2 surfaces.

188 showed Arg307 is located in a region rich in basic residues located only 12 A from the ligand binding

189 HIV-1) nucleocapsid (NC) protein contains 15 basic residues located throughout its 55-amino acid sequ

190 at the center of the region with an unusual basic residue (Lys-601).

191 Strikingly, mutation of a key basic residue (Lys-72), previously implicated in disasse

192 The arch contains basic residues (Lys-93 and Arg-100 in human FEN1 (hFEN1)

193 Furthermore, both the C-helix (contains two basic residues, Lys-104 and Arg-105) and the D-helix of

194 ntified the binding site as composed of four basic residues, Lys-69, Arg-76, Lys-80, and Lys-88.

195 Here we show that a highly conserved basic residue, Lys399, on the lateral surface of FtsY pr

196 and the second involving substitution of the basic residues, Lys402, Arg405, and Arg409, all with the

197 ha-helix III of LC3, especially in the three basic residues Lys65, Arg68, and Arg69, ensures stable i

198 Trypsin, which cuts specifically after the basic residues lysine and arginine, is the predominant e

199 could not be attributed to the presence of a basic residue (lysine) in the voltage-sensing domain of

200 The addition or removal of select, conserved basic residues markedly impacts both nucleotide-dependen

201 n and suggest that different combinations of basic residues may mediate the recognition of distinct s

202 mutation of the C-terminal membrane-proximal basic residues (MPBRs) (R310/311A) abolished the O-glyco

203 al studies indicated that both wild-type and basic-residue mutant Gag localized to the outer surface

204 Other basic residue mutations that disrupt the VP35 polymerase

205 supported by hydrogen bond interactions of a basic residue near the propionate carboxyl function of p

206 ipin-containing vesicles requires a patch of basic residues near the Ca(2+)-binding surface loops of

207 th MH susceptibility occurs at the innermost basic residue of the IS4 voltage-sensing helix, a residu

208 Mutation of each basic residue of the Myo1G Myo1PH domain reveals another

209 in a membrane environment the transmembrane basic residue of the TCR closely interacts with both of

210 and individual structural features of the 2 basic residues of arginine 449 and lysine 358, which mar

211 These results suggest that basic residues of both helices C and D of ZPI interact w

212 Together, basic residues of central beta-sheet A contribute to hep

213 CDR H3) and a number of salt bridges between basic residues of IL-13 and acidic residues of the antib

214 electrostatic interactions between conserved basic residues of MA and multiple PI(4,5)P2 and phosphat

215 analyses revealed carbonyl adducts on select basic residues of SERCA2a.

216 Mutations in the hydrophobic cap and in basic residues of the C1 domain of MgcRacGAP prevent ass

217 the heparin-binding site of ZPI, the role of basic residues of the D-helix (residues Lys-113, Lys-116

218 We replaced the basic residues of the fXIa 170 loop (Lys-170, Arg-171, A

219 rm showed a unique binding mode with the two basic residues of the ligand forming salt bridges with t

220 A conserved basic residue on the putative DNA-binding surface of CpE

221 hoinositide binding site in H7, comprised of basic residues on a surface patch and the flexible C-ter

222 his model explains diverse effects of the TL basic residues on catalysis through their effects on pos

223 minogen receptors exposing carboxyl terminal basic residues on cell surfaces have been identified.

224 h Met51 fastens like a bolt, and a groove of basic residues on either side, which bond to the acidic

225 This and the fact that basic residues on F2 and F3 are also essential for integ

226 ute electrostatically, because two conserved basic residues on one face are required for optimal memb

227 by plasminogen receptors exposing C-terminal basic residues on the macrophage surface.

228 a Deltapsi or a DeltapH; the second requires basic residues only when driven by a Deltapsi.

229 s Met >> Leu > Phe > Ala in the X1 position, basic residues or Trp in the X2 position, and Pro >> Ala

230 alanine substitution mutants indicates that basic residues outside the central basic patch are not r

231 suggest that the C-terminal helix and other basic residues outside the enzymatic cleft account for s

232 Mutations of four aromatic and basic residues: Phe(122), Arg(146), Arg(156), and Phe(16

233 PASR downward arrowF) contains only a single basic residue (position -1) that matches the preferred f

234 rt forms a loop with helical propensity, and basic residues predicted to reside on one surface of the

235 number, but not the identity or position, of basic residues present in the PBM dictates p14 export fr

236 erally, in protein-PI(4,5)P(2) interactions, basic residues promote the interaction as docking sites

237 We identified three basic residues promoting PI(4,5)P(2)-dependent Gag-membr

238 oxin, carrying a net charge of +6 e with six basic residues protruding from its surface, is attracted

239 idine residues combined with an abundance of basic residues provided challenges for generation of pep

240 ar to that of its human analogues, utilizing basic residues R18, R46, and R48.

241 upon individual mutation of three additional basic residues: R18, R35, and K46.

242 by a point mutation of the highly conserved basic residue R205A, residing in the short helix H1(T2)

243 Mutations in three basic residues (R301E/R307E/K311E) of the C2 subdomain o

244 m the Golgi complex, with a minimum of three basic residues required for efficient Golgi export.

245 bitory face of the KA1 domain as a series of basic residues residing on two conserved regions of the

246 educed CAR-T cytokine production whereas the basic residue rich sequence (BRS) of CD3epsilon promoted

247 itional mutations in the upstream stretch of basic residues (RXR motif) restored O-glycosylation and

248 ) 810 represents the essential and conserved basic residue (SPXK) required for cyclin/Cdk recognition

249 of internal cysteines in close proximity to basic residues such as histidines.

250 with at least one arginine residue and other basic residues, such as lysine or histidine.

251 Mutagenesis of a few conserved basic residues suggested a U-shaped binding path for ssD

252 axial and peripheral sites are surrounded by basic residues, suggesting an electrostatic mechanism fo

253 othesis that the electric field generated by basic residues surrounding Phe-232 is key to the polariz

254 We found that despite having many more basic residues than some chemokines, vMIP-II shares a ch

255 cyclase catalysis, we discovered a conserved basic residue that seems to act as a counter ion to the

256 This dual function is achieved by basic residues that alternately face either the membrane

257 active under low-pH conditions and cleave at basic residues that are "forbidden" in peptic digests.

258 fied by mutagenesis study, consists of eight basic residues that are located mostly at the junction o

259 coenzyme and product reveals five conserved basic residues that bind the carboxylates of the tetrapy

260 f GH130 enzymes, however, lack the conserved basic residues that bind the phosphate nucleophile, and

261 The Sds3 linker features several conserved basic residues that could potentially maintain the compl

262 Here, we report that mutations of basic residues that line phage T4 TerS (gp16) channel do

263 tagenesis revealed a cluster of aromatic and basic residues that mediate its interaction with ASIC3.

264 ibosome via three surface-exposed patches of basic residues that we propose directly interact with 16

265 cium, the S1 pocket can bind both acidic and basic residues through formation of salt bridges with th

266 inary complex with PCNA on chromatin and the basic residue to recruit CRL4(Cdt2) for substrate ubiqui

267 ase complex function, identified these other basic residues to be critical for viral RNA synthesis.

268 The overall contribution of basic residues to binding affinity is also context depen

269 minal tail (NTT) of yeast eIF1A deploys five basic residues to contact tRNAi, mRNA, or 18S rRNA exclu

270 We replaced these basic residues to evaluate their role in enzyme autopoly

271 Mutation of these basic residues to glutamine reduced cell wall binding bu

272 show that the spectrin domain uses conserved basic residues to promote the recruitment of Ase1 to the

273 By neutralization of basic residues, we identified putative PI(4,5)P(2) bindi

274 ate specificity for RXR motifs surrounded by basic residues, we performed site-directed mutagenesis s

275 osphoserine, whereas substrates lacking this basic residue were not effectively hydrolyzed.

276 using a panel of APC exosite variants where basic residues were mutated, in binding to immobilized F

277 role in nuclear localization, but additional basic residues were required for maximum shutoff activit

278 Moreover, we identified two basic residues, which protrude into the elongated pore t

279 ide bond by protonating the anchor peptide's basic residues, while leaving the N-terminal amine unpro

280 45 protein by substituting the most critical basic residues with glutamine.

281 Finally, mutation of a single basic residue within a polybasic stretch of the DEP doma

282 These results suggest that basic residues within a dimerized FtsN(Cyto) protein int

283 genesis localize this binding motif to three basic residues within Helix 1 of the E2 core domain, con

284 ional prenylation as well as by a stretch of basic residues within its carboxyl terminus.

285 Here we report that basic residues within MA are critical for directing MLV

286 Individual alanine substitutions of two basic residues within the 40s' loop, K42 and R43, abroga

287 lized by several interactions with conserved basic residues within the active site.

288 its inhibitor ENSA, by cooperative action of basic residues within the BPR.

289 Mutation of select basic residues within the C-terminal half of VP35 abroga

290 t as well as by site-directed mutagenesis of basic residues within the extracellular domain of PECAM-

291 interactions between a conserved cluster of basic residues within the first five positions of the JM

292 f the T-cell copatching study, we found that basic residues within the matrix domain of Gag are requi

293 unveiled the burial of critical aromatic and basic residues within the micelles.

294 a hydrophobic cleft, an adjacent cluster of basic residues within the N terminus, and a potential ne

295 Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding sur

296 Site-directed acidification of the basic residues within the polybasic motif of KChIP2a1 re

297 is mediated by electrostatic interactions of basic residues within the Pro-rich motif with acidic clu

298 A stretch of basic residues within the Tarbp2 protein is required for

299 proteases, seven of which cleave at specific basic residues within the trans-Golgi network, granules,

300 evious studies revealed that mutation of key basic residues within the VP35 interferon inhibitory dom