ライフサイエンスコーパス: biocatalyst

1 g., vaccines, drug delivery, imaging agents, biocatalysts).

2 ure, and demonstrated its use as a promising biocatalyst.

3 phatogenic strain of Escherichia coli as the biocatalyst.

4 ed using Candida antarctica lipase type B as biocatalyst.

5 IM lipase from Rhizomucor miehei was used as biocatalyst.

6 a lipase B, Lipozyme(R) 435, was used as the biocatalyst.

7 olerant, and effective cofactor-regenerating biocatalyst.

8 methane utilization in a promising bacterial biocatalyst.

9 amyl octanoate) using lipase Palatase as the biocatalyst.

10 ein is not the most efficient PHA polymerase biocatalyst.

11 aximize the benefit from this extremely fast biocatalyst.

12 and enhanced the reactivity of a whole cell biocatalyst.

13 reaction, underscoring its versatility as a biocatalyst.

14 ationalize the high stereoselectivity of the biocatalyst.

15 ic turnover numbers of individual whole-cell biocatalysts.

16 ntial wide application in industrial E. coli biocatalysts.

17 ed remarkable regio-promiscuity of nitration biocatalysts.

18 tion as possible sources of renewable energy biocatalysts.

19 ow cost, and self-replication and -repair of biocatalysts.

20 odologies were used in order to prepare four biocatalysts.

21 0 mM cyclohexylamine by employing whole-cell biocatalysts.

22 rformance of nicotinamide coenzyme-dependent biocatalysts.

23 ing that thermophiles have high potential as biocatalysts.

24 tential to greatly enhance the robustness of biocatalysts.

25 PS-C1 (lipase from Burkholderia cepacia) as biocatalysts.

26 drug metabolism, and have potential uses as biocatalysts.

27 classic organic reactions in the presence of biocatalysts.

28 levance for optimization of flavin-dependent biocatalysts.

29 ctive circuits for biochemical processes and biocatalysts.

30 tion of self-regenerating microbial cells as biocatalysts.

31 development of new protein therapeutics and biocatalysts.

32 ctive methods for manipulating and tailoring biocatalysts.

33 their potential for generating truly de novo biocatalysts.

34 al in origin and ends with those that employ biocatalysts.

35 late and facilitates artificial evolution of biocatalysts.

36 tform for the design of binding proteins and biocatalysts.

37 orresponding lactams by employing engineered biocatalysts.

38 locks of natural products and are attractive biocatalysts.

39 geted engineering of these enzymes as useful biocatalysts.

40 to engineer the next generation of designer biocatalysts.

41 ynthesis of halogenated natural products, as biocatalysts.

42 itions were optimized as follows: whole-cell biocatalyst 0.8 g/L, leucine concentration 13.1 g/L, tem

43 cked bed reactor filled with the immobilized biocatalysts, 52.6 mmol of TCP was continuously converte

44 pproaches has produced many metal-containing biocatalysts able to adopt the functions of native enzym

45 modification often requires large amounts of biocatalyst, adding significant costs to the process and

46 The microbial exoskeleton also protected the biocatalyst against a variety of external stressors incl

47 The engineered biocatalyst also reacts with a variety of indole analogu

48 se from Pseudomonas stutzeri was the fastest biocatalyst among all assayed, although poor discriminat

49 This platform was probed with uHTS for biocatalysts anchored to yeast with enrichment close to

50 catechuate 2,3-dioxygenase (HPCD) as a model biocatalyst and coated it with up to ten alternating lay

51 matic activity is a persistent bottleneck in biocatalyst and drug development.

52 st implementation of a nitrone synthase as a biocatalyst and establishes a novel platform for late-st

53 9:1 (v/v) were found to be the most suitable biocatalyst and medium, respectively, and significantly

54 using Shewanella oneidensis MR-1 (MR-1) as a biocatalyst and performance was assessed in terms of cur

55 ansfer accelerator, alcohol dehydrogenase as biocatalyst and polydiallyldimethylammonium chloride as

56 tron transfer (DET) between a photosynthetic biocatalyst and the anode of a MFC.

57 ity, as well as enabling the design of novel biocatalysts and biosensors.

58 gh stereo-, regio-, and chemoselective redox biocatalysts and enabling reactions under mild condition

59 atalysis, potential untapped sources of such biocatalysts and how further optimization of these enzym

60 ct to the characteristics of the immobilized biocatalysts and synthesis conditions.

61 een reaction mechanisms mediated by modified biocatalysts and synthetic catalysts.

62 ALs correlated with the distance between the biocatalysts and the solid supports, and in turn, the mo

63 neering is industrially fruitful in creating biocatalysts and therapeutic proteins, applications of a

64 ctivity recovery, enzyme loading (wt% in the biocatalyst) and the physical properties, e.g. particle

65 otein engineering, and medium engineering of biocatalysts are available, the main focus of this work.

66 Challenges related to the application of biocatalysts are discussed, including how 'green' a bioc

67 ave been made available for this purpose, no biocatalysts are known to mediate this transformation.

68 ENGase biocatalysts are now finding burgeoning application for

69 Immobilized biocatalysts are suitable for removing TCP from contamin

70 In addition, biocatalysts are used on a large scale to make specialty

71 the development of a surface display enzyme biocatalyst as an effective and renewable alternative fo

72 A tag is capable of operating as a secondary biocatalyst as well as bridging two enzymes for co-encap

73 this reason, the preparation of a library of biocatalysts as broad as possible may be a key turning p

74 neering enable the development of whole-cell biocatalysts as powerful tools in organic synthesis.

75 ers to facilitate identification of suitable biocatalysts, as well as references to recent volumes an

76 constants as much as 21-fold by running the biocatalyst at elevated temperatures ranging from 40 deg

77 ort a highly specific, convenient and robust biocatalyst based on a novel ether hydrolase enzyme, DNA

78 ndida antarctica lipase A (CAL-A) as a model biocatalyst based on a parameter study and fitting to th

79 evolution, we have engineered a hemoprotein biocatalyst based on a thermostable cytochrome c that di

80 A hydrogen evolution biocatalyst based on photosystem 1-platinum nanoparticle

81 ive of this study was to produce a composite biocatalyst, based on porous cellulosic material, produc

82 presents an opportunity to construct custom biocatalysts built in a lego-like fashion by inserting,

83 cterial communities provide a rich source of biocatalysts, but their experimental discovery by functi

84 as furnished a diverse array of halogenation biocatalysts, but thus far no examples of dehalogenating

85 ein a new approach for a magnetic core-shell biocatalyst by immobilization of Candida antarctica B li

86 In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surfac

87 lop a new concept to impart new functions to biocatalysts by combining enzymes and metal-organic fram

88 However, beyond these limitations, stable biocatalysts can be operated at higher temperatures or c

89 Whole cell biocatalysts can perform numerous industrially-relevant

90 ted to an expanded genetic code, affording a biocatalyst capable of accelerating ester hydrolysis wit

91 phototroph, Rhodopseudomonas palustris, as a biocatalyst capable of light-driven CO2 reduction to CH4

92 this approach we identified imine reductase biocatalysts capable of accepting structurally demanding

93 The development of biocatalysts capable of fermenting xylose, a five-carbon

94 Biocatalysts capable of unlocking new and efficient Diel

95 This biocatalyst closes an important gap in allowing the conv

96 prove the stability and recyclability of the biocatalyst compared to the free enzyme.

97 stereoselectivity of these cyclopropanation biocatalysts complements that of trans-(1S,2S)-selective

98 sign for continuous milk coagulation using a biocatalyst composed of immobilized animal and vegetable

99 ntinuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubula

100 The enantiopreference of the biocatalyst could also be tuned to provide either enanti

101 The biocatalyst could be used at least in five sequential ba

102 The hydrolase based biocatalyst could provide the basis for a wide spectrum

103 The new biocatalysts demonstrated high catalytic efficiency in t

104 vents per time, which is crucial to speed up biocatalyst design, and to widen our knowledge about seq

105 The advent of biocatalysts designed computationally and optimized by l

106 /d-xylose regulatory network is key for such biocatalyst development.

107 Biocatalyst discovery and directed evolution are central

108 aminations with wild-type and Q240A variant biocatalysts displaying total turnover numbers of up to

109 evelopment and application of an immobilized biocatalyst, due to the well-known advantages over solub

110 Carbon nanomaterials regenerate the biocatalysts either by direct electron transfer or redox

111 ation with whole-cell transformations, these biocatalysts enabled the gram-scale assembly of a key in

112 iotransformations, these stereocomplementary biocatalysts enabled the multigram synthesis of the chir

113 ad substrate promiscuity, and (iv) TnmH as a biocatalyst enables the development of novel conjugation

114 Using toluene dioxygenase as biocatalyst, enantiopure cis-dihydrodiol and cis-tetrahy

115 in drug discovery, clinical diagnostics, and biocatalyst engineering.

116 e the potential of TtHGXPRT as an industrial biocatalyst, enzymatic production of several dietary 5'-

117 of traits was also constructed in succinate biocatalysts (Escherichia coli strain C derivatives) and

118 ver 70 functional OYE1 variants with several biocatalysts exhibiting over an order of magnitude impro

119 elopment of myoglobin-based cyclopropanation biocatalysts featuring dramatically enhanced thermostabi

120 mer methodology employing a Lewis acid and a biocatalyst, followed by nucleophilic substitution with

121 After the treatment of Muscat wine with the biocatalyst for 20days, free monoterpenes increased sign

122 onths with the possibility of storage of the biocatalyst for 30 d in wine at 25 degrees C.

123 erase was converted into the most proficient biocatalyst for [4+2] cycloadditions known.

124 ratase from Bacillus sp. OxB-1 was used as a biocatalyst for a dehydration of aldoximes as readily av

125 roader applicability, and (3) utility of the biocatalyst for abiotic synthesis.

126 ditional ethanologenic agent and a promising biocatalyst for advanced biofuels production using ligno

127 efficient and cost-effective exoelectrogenic biocatalyst for boosting the industrial application of M

128 trocatalysts and Methanosarcina barkeri as a biocatalyst for CO2 fixation, we demonstrate robust and

129 H2-producing organism D. vulgaris is a good biocatalyst for converting formate to H2.

130 genesis and screening to afford a proficient biocatalyst for enantioselective cleavage and synthesis

131 essful de novo design of a single whole-cell biocatalyst for formal stereoselective C-H amination.

132 trogen, and energy and is considered a prime biocatalyst for groundwater bioremediation in DWTPs.

133 ggests that this enzyme could be a potential biocatalyst for industrial lipid hydrolysis and conversi

134 ese unique properties make CgNal a promising biocatalyst for industrial Neu5Ac biosynthesis.

135 nnin, leading to the production of an active biocatalyst for milk coagulation (initiation of milk clo

136 e phase transition of ELP and thus served as biocatalyst for OPs, while BSA was used to stabilize OPH

137 Laccase could act as a biocatalyst for oxygen reduction reaction along with cat

138 pendent ligation DNAzyme is implemented as a biocatalyst for the amplified detection of a target DNA

139 ducts, CloQ offers considerable promise as a biocatalyst for the chemoenzymatic synthesis of novel co

140 te the applicability of the BfrA-immobilised biocatalyst for the complete hydrolysis of concentrated

141 haromyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic a

142 the requirements for an industrial strength biocatalyst for the direct conversion of biomass to comb

143 ctrum of activities make MdP2'GT a promising biocatalyst for the industrial preparation of the corres

144 ed chemo-enzymatic pathway and an engineered biocatalyst for the multistep synthesis of an important

145 scope and limitations of using E. coli as a biocatalyst for the production of diesel-like fuels.

146 Duolite A658-Lecitase is a very suitable biocatalyst for this reaction.

147 as used to expand reaction scope, generating biocatalysts for amide bond formation from carboxylic ac

148 ce space to enable the identification of new biocatalysts for asymmetric synthesis remains both a cha

149 owards future de novo design of minimalistic biocatalysts for biotechnological and industrial applica

150 spread application of omega-transaminases as biocatalysts for chiral amine synthesis has been hampere

151 xing enzymes is a prerequisite to create new biocatalysts for diverse applications in chemistry, biot

152 ing points for the development of customized biocatalysts for diverse practical applications.

153 The use of thermophilic microorganisms as biocatalysts for electromethanogenesis was investigated.

154 port the development of engineered myoglobin biocatalysts for executing asymmetric intramolecular cyc

155 a pastoris, are investigated and compared as biocatalysts for glucose oxidation using flow injection

156 y reveals potential for their development as biocatalysts for glycodiversification.

157 athway and host selection when designing new biocatalysts for implementation by metabolic engineering

158 e a promising strategy for creating tailored biocatalysts for many synthetically useful reactions.

159 recently identified as a promising class of biocatalysts for mediating C-H aminations via nitrene tr

160 metagenomics approaches to identify natural biocatalysts for novel chemical transformations.

161 importance of selecting from both chemo- and biocatalysts for optimal results.

162 eotide phosphate, and have become attractive biocatalysts for organic synthesis.

163 ure elaboration into efficient and selective biocatalysts for organosiloxane chemistry.

164 This study therefore unveiled new routes and biocatalysts for polyketide cyclization.

165 ader peptide, making them potentially useful biocatalysts for preparation of new thiopeptide variants

166 rk components may help in the development of biocatalysts for production of fuels and chemicals from

167 ese may have potential utility as industrial biocatalysts for production of maltose.

168 heir affinity for O(2) makes them attractive biocatalysts for technological devices in which O(2) con

169 nthetic prokaryotes showing great promise as biocatalysts for the direct conversion of CO2 into fuels

170 rable interest in the development of PALs as biocatalysts for the enantioselective synthesis of non-n

171 Immobilized lipases are excellent biocatalysts for the enzymatic synthesis of short- and m

172 ly studied, and both were found as excellent biocatalysts for the production of adequate chiral inter

173 derivatives, and the potential of engineered biocatalysts for the production of quaternary centers.

174 ecific peroxygenases, which are deemed ideal biocatalysts for the selective activation of C-H bonds.

175 abled the rapid evolution of three efficient biocatalysts for the selective hydroxylation of a primar

176 zyme (OYE) family are widely used, effective biocatalysts for the stereoselective trans-hydrogenation

177 m engineering, protein (enzyme) engineering, biocatalyst (formulation) engineering, biocatalytic casc

178 regioselectivity differentiates the sophora biocatalyst from microbial rutinosidases.

179 tained their ability to protect encapsulated biocatalysts from degradation by proteases.

180 ometer, and it can efficiently isolate novel biocatalysts from metagenomic libraries by processing si

181 mine this natural diversity to obtain useful biocatalysts, generating comparably rich ortholog divers

182 n submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; stil

183 The synthetic effectiveness of these biocatalysts has been significantly increased by the pro

184 These engineered MAO-N biocatalysts have been applied in deracemization reactio

185 De novo biocatalysts have been successfully generated by computa

186 Biosensors and biocatalysts have limited applicability to the more trad

187 ble and robust construction of truly de novo biocatalysts holds promise for applications in chemical

188 ty of 22.2 g of substrate hydrolysed/gram of biocatalyst/hour.

189 Biocatalysts, however, are delicate materials that hover

190 oaches to enhancing the stability of protein biocatalysts: (i) rational design, based on knowledge of

191 With a number of biocatalysts identified that operate with complementary

192 ement scenarios targeting biocatalyst yield, biocatalyst immobilization for reuse, and elimination of

193 biocatalytic reaction may be, and trends in biocatalyst improvement through enzyme engineering are p

194 t of the enzyme and embedment of the primary biocatalyst in a silica layer.

195 limonene into alpha-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B.

196 usion, strongly affect the efficiency of the biocatalyst in each specific reaction system.

197 n strategy and implicates a potential unique biocatalyst in mccrearamycin cyclopentenone formation.

198 sfully demonstrated as a novel and efficient biocatalyst in METs such as microbial fuel cells.

199 The active biocatalyst in the anodic chamber was a mixed culture of

200 n, improved stability and the absence of the biocatalyst in the product stream.

201 rom the sea have begun to emerge as powerful biocatalysts in medicinal chemistry and total synthesis.

202 alogenases increasingly attract attention as biocatalysts in organic synthesis, facilitating environm

203 outcome of reactions entice chemists to use biocatalysts in organic synthesis.

204 6-membered tylosin macrocycle, using them as biocatalysts in the chemoenzymatic synthesis of tylacton

205 asonable step to improve the performances of biocatalysts (including both enzymes and whole-cell syst

206 m(ii) complexes as anticancer candidates and biocatalysts, including arene ruthenium complexes, polyp

207 ymes have tremendous potential as industrial biocatalysts, including in biological lignin valorizatio

208 The biocatalyst is capable of up to 1,300 turnovers, exhibit

209 2+)-dependent ligation DNAzyme as amplifying biocatalyst is presented.

210 s to identify the best-performing whole-cell biocatalysts is a low-throughput endeavor.

211 The catalytic activity of artificial iron biocatalysts is also briefly reported in order to underl

212 efficient, environment-friendly, commercial biocatalysts is highly attractive.

213 has been devoted toward improving enzymes as biocatalysts is presented.

214 than nature" NCBs and several ene reductase biocatalysts is used to indicate transfer by quantum mec

215 observations, we engineer HG4, an efficient biocatalyst (k(cat)/K(M) 103,000 M(-1)s(-1)) containing

216 roup into unprotected phenols by employing a biocatalyst (laccase), tBuOOH, and either the Langlois'

217 ous catalytic methods is presented, covering biocatalysts, Lewis acid catalysts based on boron and me

218 ntages in view of stability, volume specific biocatalyst loading, recyclability as well as simplified

219 Biocatalysts may be delicate proteins, however, once sta

220 ifferences in the performance of immobilised biocatalysts must be interpreted very carefully.

221 This study created a novel biocatalyst (named as SDFsC) by expressing the enzyme Fu

222 cellulose into soluble sugars, making them a biocatalyst of biotechnological interest for use in the

223 t low temperature (e.g., 50 degrees C) using biocatalysts of 6 commercial lipases adsorbed on hydroph

224 This carbon-silicon bond-forming biocatalyst offers an environmentally friendly and highl

225 Using a strain of Desulfuromonas as biocatalyst on the anode resulted in an acetate producti

226 inimal external risks, given that engineered biocatalysts only have improved fitness within the custo

227 The expanding "toolbox" of biocatalysts opens new opportunities to redesign synthet

228 bilization of enzymes aiming at their use as biocatalysts or biosensors.

229 nd 1, 2 &4 in Escherichia coli affords three biocatalysts over-expressing 4-8 enzymes for one-pot con

230 cellular reactivity of only 15 +/- 5 single biocatalysts per droplet could be demonstrated for the f

231 nd/or enzymes have to be tailored to improve biocatalyst performance.

232 The best CALB biocatalyst preserved 90% of the activity after 30days u

233 With resistant biocatalysts, product yields (ethanol and succinate) fro

234 Biocatalyst production was highly correlated with cost a

235 The biocatalyst, referred to as surface display laccase (SDL

236 ng among the most efficient nitrene transfer biocatalysts reported to date.

237 hree-dimensional structure of this important biocatalyst reveals that residues implicated in the enha

238 CP can influence a biocatalyst's function by altering protein backbone flex

239 extremely diverse, widespread, and versatile biocatalysts, sensors, and molecular transporters.

240 The resulting set of efficient biocatalysts showcases the tunability of hemoproteins fo

241 The resultant biocatalysts showed broad applicability toward the synth

242 By examining various biocatalyst/substrate combinations, it was demonstrated

243 s technology was applied in conjunction with biocatalysts such as ketoreductases and aminotransferase

244 a Daphnia magna model organism and a perlite biocatalyst support material demonstrate the attainable

245 OxaD is a versatile biocatalyst that converts an array of semisynthetic roqu

246 y, offers scope for the development of novel biocatalysts that are both highly active and robust.

247 Hence, de novo design of robust biocatalysts that are much simpler than their natural co

248 tase metalloenzymes are unique and important biocatalysts that are used industrially to produce high

249 Central to this challenge is the design of biocatalysts that can efficiently convert cheap lignocel

250 Carboxylases are biocatalysts that capture and convert carbon dioxide (CO

251 tion, there are increased efforts to develop biocatalysts that confer regioselectivity for site-speci

252 synthetic biology-and the robustness of the biocatalysts that convert the metabolic intermediates to

253 Biocatalysts that perform C-H hydroxylation exhibit exce

254 -dependent halogenases (FDHs) are attractive biocatalysts, their practical applications are limited b

255 teins' stability to enhance their utility as biocatalysts, therapeutics, diagnostics and nanomaterial

256 an be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before

257 ligninases promises the development of novel biocatalysts, these enzymes have largely been characteri

258 we report the creation of improved nitration biocatalysts through constructing and characterizing fus

259 biological processes rely on highly complex biocatalysts, thus limiting their industrial application

260 ective oxidations, and represent a promising biocatalyst to address this challenge.

261 le MFC used Pseudomonas aeruginosa PAO1 as a biocatalyst to generate the maximum power and current de

262 ive site makes XdINV a valuable and flexible biocatalyst to produce novel bioconjugates.

263 lum could potentially be used as a microbial biocatalyst to produce renewable fuels directly from lig

264 rthermore, it is desirable to immobilize the biocatalysts to enable ease of separation from the react

265 alcohol dehydrogenases were investigated as biocatalysts to enantioselective oxidation of racemic er

266 ase (IRED) and omega-transaminase (omega-TA) biocatalysts to establish the key stereocentres of these

267 e for catalysis, future work will tailor the biocatalysts to high-demand synthetic processes by evolv

268 f bioprocesses and the ability of engineered biocatalysts to produce designer products at high carbon

269 of novel enzymes that could be exploited as biocatalysts to rapidly access complex diterpenoid natur

270 This use of different biocatalysts to select different C-H positions contrasts

271 has significant implications for engineering biocatalysts to valorize lignin.

272 n of local photoelectrocatalytic activity of biocatalysts towards light-induced hydrogen evolution.

273 Biocatalyst turnover numbers were analyzed using rationa

274 directed evolution of an amine dehydrogenase biocatalyst via ultrahigh throughput droplet screening.

275 highlights the applicability of one class of biocatalysts viz."lipases" in synthetic transformations,

276 e evolutionary trajectory, a stereodivergent biocatalyst was also obtained for affording mirror-image

277 The criteria used to choose the appropriate biocatalyst was based on the time of coagulation in succ

278 The immobilized biocatalyst was characterized and then tested in fruit j

279 A new immobilized biocatalyst was developed using glyoxyl-agarose as suppo

280 via metal affinity immobilization, as a nano-biocatalyst was investigated.

281 The engineered biocatalyst was shown to be a thermostable, solvent tole

282 In all cases, the immobilized biocatalyst was shown to be efficient for pomegranate ju

283 The composite biocatalyst was studied by Scanning Electron Microscopy

284 le-cell transformations, the myoglobin-based biocatalyst was used for the asymmetric construction of

285 removal using cell-free bacterial enzymes as biocatalysts was investigated using crude cell lysates a

286 Performance of the biocatalysts was tested for lactose hydrolysis, and the

287 with Myceliophthora thermophila laccase, as biocatalyst, was performed in aqueous medium using an ec

288 onene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment,

289 omega-TA biocatalysts were also successfully employed for the pro

290 quences were selected, and the corresponding biocatalysts were evaluated for reactivity and selectivi

291 5h of reaction using 0.05g/mL of immobilized biocatalyst, which released 3mg/mL of reducing sugars an

292 design and directed evolution are versatile biocatalysts whose promiscuous activities represent pote

293 polyethylene glycol-modified lipases using a biocatalyst with higher stability than commercial deriva

294 e a key turning point to find an immobilized biocatalyst with improved properties when compared to th

295 ation of a toolbox of chiral-amine-producing biocatalysts with a Buchwald-Hartwig cross-coupling reac

296 g and robust scaffold for the development of biocatalysts with carbene-transfer reactivity.

297 The platform complements biocatalysts with characteristics of heterogeneous catal

298 Here, we report an approach for accessing biocatalysts with complementary selectivity that is orth

299 cm(2) using Shewanella oneidensis as a model biocatalyst without any optimization of bacterial cultur

300 Realistic improvement scenarios targeting biocatalyst yield, biocatalyst immobilization for reuse,