1 a nonspecific manner, which is confirmed in
biochemical experiments.
2 reduced to the small proton flux measured in
biochemical experiments.
3 ins to follow protein expression and perform
biochemical experiments.
4 identity change, we performed mutational and
biochemical experiments.
5 llowing immediate use of the modified RNA in
biochemical experiments.
6 ther characterized by additional genetic and
biochemical experiments.
7 e used in a myriad of diverse biological and
biochemical experiments.
8 centration is an essential component of most
biochemical experiments.
9 scription factors gained from structural and
biochemical experiments.
10 characterized by an array of biophysical and
biochemical experiments.
11 f its catalytic role, consistent with recent
biochemical experiments.
12 3 mM, consistent with extensive independent
biochemical experiments.
13 providing hypotheses for future genetic and
biochemical experiments.
14 units, we performed electrophysiological and
biochemical experiments.
15 hanism are usually inferred from a series of
biochemical experiments.
16 segments in reasonable quantity facilitates
biochemical experiments.
17 system that can be used in a wide variety of
biochemical experiments.
18 diated molecular switch, which we confirm by
biochemical experiments.
19 gths similar to those obtained from ensemble
biochemical experiments.
20 ty of this interaction was confirmed through
biochemical experiments.
21 other single-molecule rotation and ensemble
biochemical experiments.
22 ing activity of CP and promotes uncapping in
biochemical experiments.
23 hotocrosslinking of subunits, as assessed by
biochemical experiments.
24 On the basis of these genetic and
biochemical experiments,
a biosynthetic pathway for BE-7
25 We present
biochemical experiments addressing this conundrum and el
26 Biochemical experiments also demonstrated that, although
27 Our
biochemical experiments also showed that deletion of the
28 Consistent with our previous
biochemical experiments,
analysis of the culture medium
29 r of wild-type and mutant CaMKIV proteins in
biochemical experiments and cellular transcriptional ass
30 steric mode of action of 25 was confirmed by
biochemical experiments and cocrystallization with the R
31 A combination of
biochemical experiments and computer simulations shows t
32 Although
biochemical experiments and cryo-electron microscopy dat
33 f single metabolite can vary greatly between
biochemical experiments and even between instrument runs
34 Biochemical experiments and genomic sequence analysis sh
35 Biochemical experiments and high-resolution crystal stru
36 The proposed mechanism is supported by
biochemical experiments and is consistent with the effec
37 Biochemical experiments and molecular dynamics simulatio
38 We used
biochemical experiments and molecular dynamics simulatio
39 By combining
biochemical experiments and NMR analysis, we demonstrate
40 Finally,
biochemical experiments and predictions show that initia
41 Biochemical experiments and proteomic analysis of the pu
42 Further
biochemical experiments and structural analysis show tha
43 of the depupylase Dop using a combination of
biochemical experiments and X-ray crystallographic studi
44 poptosome components, elegant structural and
biochemical experiments,
and analyses of the apoptosome
45 Sequence comparisons,
biochemical experiments,
and studies with mutants in tra
46 Here we show the results of
biochemical experiments as follows: (i) we demonstrate t
47 The
biochemical experiments assessing the hydrolysis of seve
48 In
biochemical experiments both phosphatases were able to r
49 sired results are achieved, or computed from
biochemical experiments carried out in vitro.
50 We characterize these residues using
biochemical experiments combined with high-resolution cr
51 Mutagenesis and
biochemical experiments confirm that C-terminal residues
52 fully formed only in the binary complex, and
biochemical experiments confirm this induced-fit behavio
53 Biochemical experiments confirm this interaction.
54 Biochemical experiments confirmed direct CADTK phosphory
55 Focused genetic and
biochemical experiments confirmed several conjectures ab
56 Biochemical experiments confirmed that some of these Pre
57 Biochemical experiments confirmed that the selected pept
58 Genetic and
biochemical experiments consistently demonstrated that t
59 Here,
biochemical experiments critical to testing this model w
60 usekeeping roles in Streptomyces avermitilis
Biochemical experiments define the attributes of an opti
61 Our
biochemical experiments defined the C. elegans cohesin c
62 Genetic and
biochemical experiments demonstrate that increased flux
63 Additional
biochemical experiments demonstrate that intact MAGUKs d
64 h in vivo and in vitro ChIP-Seq analysis and
biochemical experiments demonstrate that modulation of S
65 on of rsbN causes precocious sporulation and
biochemical experiments demonstrate that RsbN functions
66 Biochemical experiments demonstrate that stabilization o
67 These
biochemical experiments demonstrate that the anti-APC pe
68 Complementary
biochemical experiments demonstrate that the core U2AF h
69 NMR and
biochemical experiments demonstrate that the OCRE domain
70 t Pkd1l1 and Pkd2 localise to the cilium and
biochemical experiments demonstrate that they can physic
71 Polycomb complex 2 (PRC2) and H3K27me3, and
biochemical experiments demonstrate the ability of RBFox
72 Proteomic and
biochemical experiments demonstrate the binding of wildt
73 Biochemical experiments demonstrate the presence of AsnR
74 In addition,
biochemical experiments demonstrated increased expressio
75 Biochemical experiments demonstrated that Dam1p binds di
76 Structural and
biochemical experiments demonstrated that nsp11 exists m
77 Biochemical experiments demonstrated that PHF11 stimulat
78 rescence cross-correlation spectroscopy, and
biochemical experiments demonstrated that the formation
79 Biochemical experiments demonstrated that the luminal do
80 Cell culture aggregation assays and
biochemical experiments demonstrated the ability of Neur
81 Biochemical experiments demonstrated the occurrence of b
82 In this report we present structural and
biochemical experiments demonstrating that formation of
83 protein function from sequence is useful for
biochemical experiment design, mutagenesis analysis, pro
84 U2AF subunits associate in a tight complex,
biochemical experiments designed to address the requirem
85 hich have also become indispensable tools in
biochemical experiments designed to probe antagonist bin
86 Biochemical experiments determined that Jak2 and tubulin
87 Thus, the
biochemical experiments do not completely recapitulate t
88 Biochemical experiments establish a direct physical inte
89 Resequencing, expression analysis, and
biochemical experiments focused on one such locus (CYP39
90 it can be used to develop hypotheses guiding
biochemical experiments for establishing the role of the
91 Our
biochemical experiments further show that Sven6563 has a
92 Biochemical experiments further validate our model for F
93 1 based on genetic evidence, but conflicting
biochemical experiments have created uncertainty regardi
94 Recent
biochemical experiments have demonstrated that in the pr
95 Genetic and
biochemical experiments have demonstrated that the genes
96 Biochemical experiments have established that the metabo
97 Genetic and
biochemical experiments have found that increased modifi
98 Recent
biochemical experiments have implicated protein kinase C
99 Although
biochemical experiments have implicated RecJ exonuclease
100 Previous
biochemical experiments have indicated that the binding
101 Numerous
biochemical experiments have invoked a model in which B-
102 However, genetic and
biochemical experiments have recently demonstrated that
103 Independent
biochemical experiments have shown that MotCF binds to o
104 Biochemical experiments have shown that myosin-I membran
105 Concordant with
biochemical experiments,
however, MKK4 occupied compartm
106 Computing analysis and
biochemical experiments identified that constitutive Anx
107 Consistent with this interpretation,
biochemical experiments identify the upstream MAPKKKs Sl
108 Biochemical experiments illuminated a mechanism of inter
109 Accompanying
biochemical experiments illustrate that NP indeed has a
110 Genetic and
biochemical experiments implicate RecJ exonuclease in ho
111 crofluidic circuits that are able to perform
biochemical experiments in a parallel and autonomous man
112 Here, we conducted cell synchronization and
biochemical experiments in lung adenocarcinoma cells, re
113 roteins have been well characterized through
biochemical experiments in metazoans, their functions in
114 We explain observations from previous
biochemical experiments in the light of results obtained
115 Biochemical experiments in vitro indicate that secreted
116 e T362I RdRp exhibits a mutator phenotype in
biochemical experiments in vitro.
117 Biochemical experiments in yeast suggest a possible mech
118 Biochemical experiments including site-directed mutants
119 Genetic and
biochemical experiments,
including artificial induction
120 can be used for a variety of biophysical and
biochemical experiments,
including studies of DNA repair
121 Biochemical experiments indicate that activator proteins
122 S2 cell
biochemical experiments indicate that bantam regulates t
123 Biochemical experiments indicate that Bdf1 competes with
124 Biochemical experiments indicate that both catalyze fork
125 Biochemical experiments indicate that Ing4 is a subunit
126 Biochemical experiments indicate that peptides bound in
127 Genetic analysis, colocalization, and
biochemical experiments indicate that Prm3p interacts di
128 Biochemical experiments indicate that RECQL4 specificall
129 Genetic and
biochemical experiments indicate that Rrp6p interacts wi
130 Further, recent
biochemical experiments indicate that the Aux/IAA protei
131 Biochemical experiments indicate that the basic function
132 Biochemical experiments indicate that the charge state o
133 Chromatin immunoprecipitation and
biochemical experiments indicate that the chromodomain o
134 he conserved nuclease domains, and extensive
biochemical experiments indicate that the substrate spec
135 Biochemical experiments indicate that transcriptional el
136 Genetic and
biochemical experiments indicate that Ybp1 is rate-limit
137 Biochemical experiments indicated a specific interaction
138 Biochemical experiments indicated that cytoplasmic Cx43
139 Biochemical experiments indicated that Sec3p directly in
140 Limited proteolysis and
biochemical experiments indicated that the coat protein
141 A series of
biochemical experiments indicated that these miniprotein
142 f the Ca2+ sensor region (Cas) determined in
biochemical experiments is equal to 0.23 microM Ca2+ for
143 phosphorylation site, observed previously in
biochemical experiments,
is enabled by a network of cons
144 Using a series of biophysical and
biochemical experiments,
it has been demonstrated that t
145 Early
biochemical experiments measuring nearest neighbor frequ
146 ed in helices 90 and 92 were consistent with
biochemical experiments measuring the RNA binding and AT
147 rophysiological and fluorescence recordings,
biochemical experiments,
mutagenesis and molecular dynam
148 Nuclear magnetic resonance and
biochemical experiments now show that Tlg2p and Pep12p,
149 ide redox switch in the human BK channel and
biochemical experiments of heme, CO, and HO2 binding to
150 In
biochemical experiments on MsbA from Escherichia coli, w
151 In this study, based on prior
biochemical experiments on VKC and VKOR, we propose a he
152 Genetic and
biochemical experiments over the past decade have identi
153 tails of FtsY recruitment and, together with
biochemical experiments,
pinpoints G83 as the key RNA re
154 Genetic and
biochemical experiments previously demonstrated that the
155 Together, these NMR and
biochemical experiments provide additional insight into
156 Biochemical experiments provide evidence that NaTrxh spe
157 The results, together with recent
biochemical experiments,
provide support for a mechanism
158 arison of both structures, complemented with
biochemical experiments,
provides critical insights into
159 nction and provide information for designing
biochemical experiments require knowledge of the complet
160 Moreover, in vivo interference and
biochemical experiments reveal a key function for multip
161 Biochemical experiments reveal that the Rgf3 C-terminus
162 Immunofluorescence and
biochemical experiments reveal that this redistribution
163 Additional genetic and
biochemical experiments revealed a close relationship be
164 tracellular GABA complemented by a series of
biochemical experiments revealed a reduction of GAT acti
165 Combined cell imaging and
biochemical experiments revealed a robust increase in th
166 Structure-based
biochemical experiments revealed additional determinants
167 Moreover,
biochemical experiments revealed an association of the a
168 Furthermore, whole-cell recordings and
biochemical experiments revealed direct SVZ NSC response
169 ired for optimal Treg induction, and further
biochemical experiments revealed how TCR signaling stren
170 Biochemical experiments revealed that cortactin co-immun
171 Our parallel
biochemical experiments revealed that inductions of the
172 Biochemical experiments revealed that Lnk directly binds
173 Biochemical experiments revealed that RGS7 binds to a co
174 Genetic and
biochemical experiments revealed that the epidermal grow
175 Biochemical experiments revealed that the Perd intracell
176 A series of
biochemical experiments revealed that the variation in f
177 Biochemical experiments revealed that TLR11 and TLR12 di
178 The crystal structure, together with
biochemical experiments,
reveals an unexpected four-stra
179 Our structure, supported by
biochemical experiments,
reveals that the SRP RNA adopts
180 Biochemical experiments show Dhh1p is preferentially ass
181 Biochemical experiments show that an IcmF in which the b
182 Biochemical experiments show that both TthCsm and Staphy
183 Biochemical experiments show that CDH23 homodimers inter
184 Biochemical experiments show that coatomer directly part
185 Biochemical experiments show that Ctf19c members associa
186 However,
biochemical experiments show that PATJ associates with b
187 Biochemical experiments show that peptides from ALIX and
188 Biochemical experiments show that PKD2 physically intera
189 Nevertheless,
biochemical experiments show that scaRNAs are present at
190 Biochemical experiments show that SmpA is involved in ma
191 Biochemical experiments show that the purified kinase in
192 Isothermal titration calorimetry and
biochemical experiments show that the two Ca(2+)/CaMs in
193 essary and sufficient for VP1 binding, while
biochemical experiments show that VP3 attachment has no
194 Our
biochemical experiments show that WRN and DNA2 interact
195 Genetic and
biochemical experiments show that, when released from th
196 Biochemical experiments showed that constitutive phospho
197 orescent protein-DIM/DWF1 fusion protein and
biochemical experiments showed that DIM/DWF1 is an integ
198 Genetic and
biochemical experiments showed that EcfO was located in
199 Consistent with this prediction,
biochemical experiments showed that PAGE4 preferentially
200 Biochemical experiments showed that Rac1 associates with
201 Physiological and
biochemical experiments showed that rods from mice with
202 A series of
biochemical experiments showed that the double mutations
203 Biochemical experiments showed that the major PDGFbetaR
204 Thus, these structures explain recent
biochemical experiments showing that M. jannaschii ProRS
205 Here we report
biochemical experiments showing that PF-3450074 (PF74),
206 Biochemical experiments suggest how scanning and engagem
207 Results from crystallographic and
biochemical experiments suggest that CF I(m)25 makes use
208 Genetic, cytological, and
biochemical experiments suggest that Clp1p/Flp1p functio
209 Biochemical experiments suggest that general acid cataly
210 Biochemical experiments suggest that only CETP can trans
211 Biochemical experiments suggest that several warfarin-re
212 Biochemical experiments suggest that TFIIA is important
213 Because genetic and
biochemical experiments suggest that the processes of br
214 oxic lesion 3-methyladenine and accompanying
biochemical experiments suggested that AlkA actively int
215 Biochemical experiments suggested that BLM resides in a
216 Microscopy and
biochemical experiments suggested that expression of KMS
217 Functional and
biochemical experiments suggested that PINS and BINS are
218 Biochemical experiments support a directional sliding of
219 NMR and
biochemical experiments support a model in which the 5'U
220 Additional
biochemical experiments support a model whereby a single
221 Genetic and
biochemical experiments support a structural arrangement
222 These
biochemical experiments support the idea that yeast poly
223 RNA interference and
biochemical experiments support the model that ephrin-B3
224 n transcriptional analysis are indirect, but
biochemical experiments supported some of these deductio
225 remains uncertain with electrophysiology and
biochemical experiments supporting both a tetramer with
226 Structural analysis,
biochemical experiments,
surface electrostatics, and seq
227 We now report
biochemical experiments testing the interaction of E. co
228 demonstrate in cell culture, in vivo, and in
biochemical experiments that Akt regulation by Par-4 is
229 These results support genetic and
biochemical experiments that have implicated Bcl10 and i
230 ure of the Kv7.4 A-domain Tail together with
biochemical experiments that show that the domain is a s
231 Lastly, we demonstrate via in vitro
biochemical experiments that the APOBEC3B protein can de
232 ensional crystal structures and results from
biochemical experiments,
the phenotypes produced by thes
233 otein complexes have been identified through
biochemical experiments,
the precise molecular details a
234 Together with
biochemical experiments,
these data indicate that MTF1 u
235 ducin (PDC) has been shown in structural and
biochemical experiments to bind the Gbetagamma subunit o
236 dings, we present mass spectrometry data and
biochemical experiments to demonstrate that this lysine
237 Here we use single-molecule and bulk
biochemical experiments to determine how Cas9-RNA interr
238 the relatively much slower progress in using
biochemical experiments to determine their functions, it
239 d on these structural features, we performed
biochemical experiments to examine the interactions of D
240 atics, genetics, heterologous expression and
biochemical experiments to identify and characterize the
241 roprotective drug and carried out additional
biochemical experiments to identify its mechanism of act
242 ased expression platform along with in vitro
biochemical experiments to identify the enzyme that cata
243 Biochemical experiments to isolate pUL6 were carried out
244 has also been implicated through genetic and
biochemical experiments to play a role in DNA repair pro
245 This is accompanied by
biochemical experiments to probe the physiological impli
246 We then use a variety of
biochemical experiments to show that O-GlcNAc in general
247 Here we use genetic and
biochemical experiments to show that the essential ATPas
248 proteins and performed in vitro and in vivo
biochemical experiments to test the physical model.
249 t al. (2017) use advanced mouse genetics and
biochemical experiments to unravel the ligand-specific a
250 Here we used a combination of in vitro
biochemical experiments together with phylogenetic compa
251 A series of
biochemical experiments tracked the specific location of
252 Exposure to genetic and
biochemical experiments typically occurs late in one's a
253 more direct fashion, we designed a series of
biochemical experiments using a modified version of the
254 ural work presented here in combination with
biochemical experiments using artificial tRNA or artific
255 Because
biochemical experiments using both purified RAG proteins
256 Biochemical experiments using Chd1p purified from yeast
257 dy of evidence derived largely from in vitro
biochemical experiments using purified proteins, cell-fr
258 Biochemical experiments using the MnSOD-K68Q Ac-mimic, o
259 In
biochemical experiments,
UvrA bound to heteroduplex subs
260 In a series of
biochemical experiments we demonstrate the following fin
261 Through embryological and
biochemical experiments we find that: (1) CV2 functions
262 In
biochemical experiments,
we confirmed the specificity of
263 In a series of
biochemical experiments,
we demonstrate a specific assoc
264 In
biochemical experiments,
we demonstrate that mutant ATX3
265 Using various
biochemical experiments,
we demonstrate that the mechani
266 By combining computer simulations with
biochemical experiments,
we dissect the underlying struc
267 Based on genetic and
biochemical experiments,
we establish that the UbiI prot
268 ing a combination of genetic, molecular, and
biochemical experiments,
we establish that, in the absen
269 Through protein fractionation and
biochemical experiments,
we find that Ku70/Ku80 and DNA-
270 Using mutational analysis and
biochemical experiments,
we have discovered a previously
271 In this study, using quantitative
biochemical experiments,
we have tested the role of a li
272 Based on structural analyses and genetic and
biochemical experiments,
we identify an alpha-helical sw
273 electron microscopy (EM), bioinformatics and
biochemical experiments,
we identify two highly conserve
274 To complement the
biochemical experiments,
we investigated the effects of
275 ants in skeletal muscle fibers with in vitro
biochemical experiments,
we investigated the molecular d
276 ation spectroscopy, respectively) as well as
biochemical experiments,
we obtained two novel findings;
277 phy, electron microscopy, and functional and
biochemical experiments,
we reveal the molecular details
278 Combining structural and
biochemical experiments,
we show for the first time that
279 and sequencing (ChIP-seq) and complementary
biochemical experiments,
we show that AR-Vs display a bi
280 RAP) and photoactivatable probes, coupled to
biochemical experiments,
we show that COPI subunit delta
281 omparisons to dynamin knock-out synapses and
biochemical experiments,
we suggest that synucleins act
282 A series of
biochemical experiments were carried out to characterize
283 l cells were treated with hydrogen peroxide,
biochemical experiments were conducted, and a proteome-w
284 Biochemical experiments were used to examine point mutat
285 P are validated in extensive mutagenesis and
biochemical experiments,
which also provide a thorough c
286 Biochemical experiments with Acf1-deficient embryo extra
287 Here we employed computational modeling and
biochemical experiments with model cell lines and thymoc
288 Biochemical experiments with model nucleosomes demonstra
289 Based on
biochemical experiments with modified oligonucleotides,
290 appeared to be much faster than observed in
biochemical experiments with muscle actin.
291 In this study, we combined
biochemical experiments with observations in egg chamber
292 Biochemical experiments with purified recombinant protei
293 Here, based on the results of
biochemical experiments with purified wild-type and vari
294 Biochemical experiments with recombinant mouse enzymes d
295 e ribosome provided significant insights but
biochemical experiments with RelE were required to clear
296 Biochemical experiments with rhodopsin, cone visual pigm
297 Biochemical experiments with RNase PH demonstrated that
298 Biochemical experiments with synaptosomes from Slc38a1 k
299 Biochemical experiments with the use of recombinant DLP1
300 in a form that is suitable for quantitative
biochemical experiments with yields of 5 and 0.5 mg per