ライフサイエンスコーパス: biocytin

1 c injections of the anterograde tract tracer biocytin.

2 ere determined by intracellular injection of biocytin.

3 l types based on intracellular labeling with biocytin.

4 t the ascending axon following labeling with biocytin.

5 iol-specific reagent 3-(N-maleimidopropionyl)biocytin.

6 examined using the anterograde transport of biocytin.

7 and verified in 34/97 cells by staining with biocytin.

8 a catalytic base to remove the N1' proton of biocytin.

9 iol-reactive agent, 3-(N-maleimidylpropionyl)biocytin.

10 ace labeling using 3-(N-maleimidylpropionyl) biocytin.

11 ed after filling with the intracellular dye, biocytin.

12 stained intracellularly with neurobiotin or biocytin.

13 n and was then intracellularly injected with biocytin.

14 rade transport of iontophoretically injected biocytin.

15 After recordings neurones were filled with biocytin.

16 s model by using intracellular injections of biocytin.

17 ngle cells were intracellularly labeled with biocytin.

18 ns were verified by filling the neurons with biocytin.

19 ng recording, we filled habenular cells with biocytin.

20 died electrophysiologically were filled with biocytin.

21 by accessibility to 3-N-maleimidyl-propionyl biocytin.

22 ere localized by using the retrograde tracer biocytin.

23 the reaction with 3-(N-maleimidylpropionyl)-biocytin.

24 , a combination of nerve-tracing techniques [biocytin, 1,1;-dioctadecyl-3,3,3;, 3;-tetramethyl-indoca

25 Biocytin abundantly labeled MOC collaterals which entere

26 Biocytin accumulation in nascent discs, detected by fluo

27 the horizontal semicircular canal nerve with biocytin after nerve regrowth.

28 e direction in which malonyl-CoA reacts with biocytin (an analog of the biotin carboxyl carrier prote

29 ns in which specific cells were labeled with biocytin and 3,3'-diaminobenzidine.

30 Terminal labeling after biocytin and BDA injections into the ganglion was found

31 cortex by using intracortical injections of biocytin and carbocyanine dye (DiI).

32 xtracellularly, labeled juxtacellularly with biocytin and characterized for the presence of choline a

33 rescent labeling of the intracellular tracer biocytin and confocal microscopy.

34 ssessed by measuring diffusional transfer of biocytin and Cy3.

35 nterograde transport of biotinylated tracers biocytin and dextran-amine (BDA) with glutamate immunohi

36 We labeled these neurons with biocytin and examined them by light and electron microsc

37 rade and retrograde tracing experiments with biocytin and fluorescently labeled dextran amines indica

38 erent neurons were labeled via injections of biocytin and horseradish peroxidase into the crossed oli

39 shown in granule cells filled in vitro with biocytin and in vivo with the anterograde lectin tracer

40 gh anterior nerves with the neuronal tracers biocytin and Lucifer Yellow.

41 The cells were filled with biocytin and morphologies were reconstructed from three

42 cedure using nickel-enhanced DAB (black) for biocytin and non-enhanced DAB (brown) for PV.

43 complimentary light microscopic anterograde biocytin and retrograde horseradish peroxidase experimen

44 e revealed after extracellular injections of Biocytin and rhodamine-conjugated biotinylated dextran a

45 teine variants with 3-(N-maleimidylpropionyl)biocytin and scored accessibility to extracellular strep

46 e filled individual CA1 pyramidal cells with biocytin and serially reconstructed dendrites and dendri

47 rium-ordered with malonyl-CoA binding before biocytin and the binding of malonyl-CoA to carboxyltrans

48 Characterized units were filled with biocytin and visualized with an antibody enhanced diamin

49 of 5 x 10(-8) M for biotin, 1 x 10(-7) M for biocytin, and 2 x 10(-6) M for desthiobiotin, and it ser

50 , N-ethylmaleimide, 3-(N-maleimidopropionyl)-biocytin, and 7-diethylamino-3-(4'-maleimidylphenyl)-4-m

51 xamined by using the carbocyanine probe DiI, biocytin, and biotinylated dextrin amine (BDA).

52 A competitive binding assay for biotin, biocytin, and desthiobiotin utilizing a genetically engi

53 al neurons were labeled intracellularly with biocytin, and their patterns of local axonal arborizatio

54 Double-labeling experiments (biocytin backfill x serotonin immunoreactivity) of the t

55 onal regeneration after CBC lesions, we used biocytin backfills of CBCs followed by fluorescence labe

56 d patch-clamp recordings in brain slices and biocytin-based neuronal reconstructions.

57 ment with a bulk determination of the avidin-biocytin binding ratio.

58 methodology has been tested with the avidin-biocytin binding system for which the best-fit distribut

59 e sulfhydryl reagent 3-(N-maleimidopropionyl)biocytin (biotin-maleimide) was evaluated by Western blo

60 wing extracellular injections of the tracers biocytin, biotinylated dextran amine, and wheat germ agg

61 Gross biocytin/biotinylated dextran amine (BDA) injections int

62 T(alpha) peptides carrying maleimido-butyryl-biocytin by avidin-agarose chromatography; and (v) ident

63 ell patch-clamp recordings, were filled with biocytin by diffusion from the patch electrode.

64 ver, by using triple immunofluorescence (for biocytin, calcium-binding proteins, and neuropeptides) i

65 Using patch-clamp recordings and biocytin cell filling in acute brain slices, we assessed

66 er VI of PR, using whole-cell recordings and biocytin cell fills in horizontal rat brain slices.

67 aration by using intracellular labeling with biocytin combined with choline acetyltransferase (ChAT)

68 adish peroxidase/cholera toxin mixture, or a biocytin compound for neuronal uptake and transport.

69 double labeling of fibers and terminals with biocytin conjugated to Alexa Fluor and confocal imaging.

70 ide moiety of the reagent, maleimido-butyryl-biocytin, containing a biotinyl group; (iv) trypsin degr

71 ditional cells, which had been injected with biocytin during the electrophysiological tests, were sho

72 nd normotopic granule cells were filled with biocytin during whole-cell patch clamp recording in hipp

73 We used intracellular injection of biocytin, extracellular injection of biotinylated dextra

74 recurrent excitatory axon arbors from single biocytin-filled CA3 pyramidal cells were reconstructed.

75 Light microscopic analysis of 11 biocytin-filled cells showed that mossy cell axon arbors

76 d from electrophysiologically characterized, biocytin-filled cells; the two cell types had only minor

77 Paired intracellular recordings with biocytin-filled electrodes and subsequent light and elec

78 t-seal, whole-cell recordings were made with biocytin-filled electrodes from rat lumbar spinal cord s

79 Using biocytin-filled electrodes we recorded R-LM interneurons

80 Sprouted axon collaterals of biocytin-filled granule cells projected from the hilus o

81 infancy, the dendritic and axonal arbors of biocytin-filled hippocampal pyramidal cells were reconst

82 vitro recordings and 3-D reconstructions of biocytin-filled interneurons at different postnatal ages

83 in CA1, dual intracellular recordings using biocytin-filled microelectrodes in slices of adult rat h

84 Reconstructions of biocytin-filled MSNs revealed that the physiological div

85 Biocytin-filled multipolar and pyramidal cells displayed

86 rophysiological properties and morphology of biocytin-filled neurones.

87 Histological analysis of biocytin-filled neurons revealed that both uniaxonal neu

88 rophysiology and neuronal reconstructions of biocytin-filled neurons to compare and contrast the elec

89 Three-dimensional reconstructions of biocytin-filled neurons, performed after the patch-clamp

90 Examination of the axon arbors of biocytin-filled PGN neurons often revealed the presence

91 address this, we examined the morphology of biocytin-filled relay cells recorded in dLGN of mice.

92 re evaluated and, in representative neurons, biocytin-filled structures were quantified.

93 ugh the use of selective opioid agonists and biocytin-filled whole-cell electrodes, interneurons poss

94 ue was addressed with combined intracellular biocytin filling and whole-cell patch clamp recordings o

95 d with whole cell patch clamp recordings and biocytin filling in in vitro hippocampal slice preparati

96 ell patch clamp recordings and intracellular biocytin filling in in vitro hippocampal slice preparati

97 ted for whole-cell patch-clamp recording and biocytin filling in transverse brainstem slices from rat

98 1 region of adult rat hippocampal slices and biocytin filling of synaptically connected cells were us

99 interneurons, intracellular recordings with biocytin filling were made in adult hippocampal slices.

100 ns at comparable quality to state-of-the-art biocytin fillings, and demonstrate feasibility of long t

101 ran amines, rhodamine-linked dextran amines, biocytin, fluorogold, and rhodamine-linked latex beads),

102 and postsynaptic neurones were labelled with biocytin, followed by correlated light and electron micr

103 ta in rat brain slices and labeled them with biocytin, followed by immunocytochemical staining for pa

104 by using the anterograde axonal transport of biocytin following cortical microinjections.

105 Retrograde transport of biocytin following its ejection into stratum lucidum of

106 characterized by intracellular injection of biocytin following the electrophysiological recordings.

107 ition, the recorded neurons were filled with biocytin for morphological examination.

108 y preselected neurons that were labeled with biocytin for subsequent anatomical reconstructions.

109 se localization of anterogradely transported biocytin from the abducens nucleus and the ventral later

110 t are anterogradely labelled by transport of biocytin from the riMLF are immunoreactive to GABA, glut

111 ty-tagged with either biotin-LC-hydrazide or biocytin hydrazide, which are known to label carbonyl gr

112 Systemic administration of biotin or biocytin hydrochloride did not alter the appearance or n

113 Biocytin-Iabeled fibers traveled to the Vmo, VII, XII, a

114 in the last study, the CF was injected with biocytin in both sexes to eliminate its motoneurons from

115 ade by spiny hilar interneurons labeled with biocytin in gerbils in vivo.

116 eurons labeled by intracellular injection of biocytin in hemisected lumbosacral spinal cords in vitro

117 Tracing studies with biocytin in in vitro human hippocampal slices indicated

118 layer 2/3 resulting from focal injections of biocytin in layer 4 show an orientation-specific axial b

119 After recording, biocytin in recording electrode was inotophorized into r

120 le cells (DGCs) by intracellularly-injecting biocytin in slice preparations that were obtained from t

121 ntracellular and extracellular labeling with Biocytin in the medial superior olive (MSO) in brainstem

122 ystem for which the best-fit distribution of biocytins in the sample puncta was in good agreement wit

123 ng and the latter by orthograde transport of biocytin injected into cortical area 17, 18, or 19.

124 Biocytin injected into the superficial layers of the OT

125 iculate terminals by orthograde transport of biocytin injected into the visual cortex and identified

126 Biocytin injection and subsequent immunohistochemical la

127 ods in neurons identified morphologically by biocytin injection in the ENS.

128 DB neurons using intracellular recording and biocytin injection in vitro.

129 onal branches of MOC neurons were labeled by biocytin injections at the floor of the fourth ventricle

130 Biocytin injections in adults revealed clusters of retro

131 Biocytin injections into dysgranular parietal insular co

132 Biocytin injections into granular parietal insular corte

133 Biocytin injections into the motor layer labeled vagal r

134 Biocytin injections revealed complete disruption of olig

135 uct neurons, we used Golgi impregnations and biocytin injections, as well as DiOlistics, a novel tech

136 Here, by using small biocytin injections, we demonstrate that distinct intrin

137 Using biotinylated dextran amine (BDA)/biocytin injections, we describe the cortical projection

138 Injections of biocytin into head and limb areas of secondary somatosen

139 By placing injections of biocytin into layer VI of tree shrew striate cortex, we

140 Extracellular injections of biocytin into the anteroventral cochlear nucleus (AVCN)

141 fibers in the ICX, labelled by injections of biocytin into the central nucleus of the inferior collic

142 The injections of biocytin into the dentate granule cell layer labeled neu

143 To identify TRN terminals, we injected biocytin into the visual sector of the TRN to label term

144 ricted injections of the anterograde tracer, biocytin, into Barrington's nucleus labeled varicose fib

145 Biocytin introduced into individual neurons during patch

146 Electron microscopic images of these biocytin labeled expansions revealed that they were larg

147 ied the bipolar cells by selective uptake of biocytin, labeled the cones with peanut agglutinin, and

148 Both biocytin-labeled and unlabeled axon terminals formed exc

149 Three individual biocytin-labeled cells had electrophysiological properti

150 The biocytin-labeled cells selectively contacted cones whose

151 ing for GABA to distinguish TRN terminals as biocytin-labeled GABA-positive terminals and to distingu

152 Individual biocytin-labeled GPHNs in hippocampal slices from epilep

153 The apical dendrite of 86% of the biocytin-labeled HEGCs extended to the outer edge of the

154 Biocytin-labeled mossy fiber axons revealed two characte

155 PV interneurons and biocytin-labeled PNs were visualized with a two-color im

156 y appeared in the neuropil, colocalized with biocytin-labeled primary vestibular fibers and vestibula

157 (A) receptor-mediated IPSCs were measured in biocytin-labeled pyramidal neurons in the PCC/RSC and pa

158 al ganglion cells was revealed by retrograde biocytin labeling from the optic disc.

159 using whole-cell patch-clamp recordings and biocytin labeling in brainstem slices.

160 been examined using 3-N-maleimidyl-propionyl biocytin labeling in cells permeabilized by polymyxin B

161 of studies using intracellular recording and biocytin labeling in hippocampal slices from macaque mon

162 s end, we performed whole-cell recording and biocytin labeling of PrS neurons in layer (L)II and LIII

163 Direct recordings and biocytin labeling revealed two major types of interneuro

164 n; (2) whole-cell patch clamp recordings and biocytin labeling to assess excitability of striatal MSN

165 uridine (BrDU) pulse labeling, intracellular biocytin labeling, and immunocytochemistry to determine

166 nstrated by anterograde autoradiographic and biocytin labeling.

167 ic slices, in conjunction with intracellular biocytin labeling.

168 19-d-old rats using whole-cell recording and biocytin labeling.

169 Biocytin-labeling of recorded neurons revealed that wide

170 We have used intracellular recording and biocytin-labeling techniques in the entorhinal slice pre

171 enotype was confirmed via electrophysiology, biocytin-labeling, histology, and in situ hybridization,

172 Morphological observations of biocytin-labelled neurones confirmed our recordings were

173 The three-dimensional morphology of 63 biocytin-labelled neurones was used to construct compart

174 dentified as AVP neurones, and ten of the 33 biocytin-labelled PVN neurones were identified as AVP or

175 d soma-dendritic distribution of anterograde biocytin-labelled rostral interstitial nucleus of the me

176 Seventeen of the 24 biocytin-labelled SON magnocellular neurones were identi

177 ons by combining intracellular recording and biocytin labelling with laser-scanning photostimulation.

178 sitive to the thiol reagent maleimidobutyryl biocytin (MBB).

179 e-permeable reagent 3-(N-maleimidylpropionyl)biocytin (MPB) and the -impermeable reagent 4-acetamido-

180 iol-reactive reagent 3-(N-maleimidopropionyl)biocytin (MPB) supported a topology model in which two h

181 sulfhydryl reagent 3-(N-maleimido-propionyl) biocytin (MPB) was determined.

182 ulfhydryl reagent, 3-(N-maleimidylpropionyl)-biocytin (MPB), to prevent yCc from binding at the site

183 ulfhydryl reagent, 3-(N-maleimidylpropionyl) biocytin (MPB).

184 sulfhydryl reagent 3-(N-maleimidylpropionyl) biocytin (MPB).

185 Focal injections of the tracers biocytin or biotinylated dextran amine (BDA) into the MG

186 Focal injections of the anterograde tracers biocytin or biotinylated dextran amine were made into th

187 tracing methods: iontophoretic injections of biocytin or biotinylated dextran-amine (BDA) were made i

188 rmal autopsy tissue and the neuronal tracers biocytin or biotinylated dextrans in in vitro slice prep

189 Neurons labeled with biocytin or neurobiotin were classified on the basis of

190 tions of Phaseolus vulgaris-leucoagglutinin, biocytin, or dextran-rhodamine in the medial superior ol

191 r both the maximum velocity (V) and the (V/K)biocytin parameters decreased at low pH.

192 Tract-tracing experiments by using biocytin, pressure-injected into the VLF, showed that on

193 enzyme with a pK of 6.2 or lower in the (V/K)biocytin profile and 7.5 in the V profile must be unprot

194 Unilateral application of biocytin restricted to the region defined by the somata

195 In the present study, the biocytin retrograde tracing technique was used to label

196 Intracellular labeling of astrocytes with biocytin revealed that CA1 astrocytes are characterized

197 Intracellular injection of biocytin revealed that neurons could be characterized in

198 Intracellular filling of Imc neurons with biocytin revealed two cell types.

199 Anterograde transport of biocytin showed up to 1 mm of outgrowth by regenerating

200 Reconstructions were made of 20 biocytin-stained cells, which had been previously studie

201 st platform combining patch clamp recording, biocytin staining and single-cell RNA-sequencing (Patch-

202 In vitro whole-cell recordings and biocytin staining demonstrated the existence of a novel

203 R) cells, was studied by using intracellular biocytin staining in brain slices obtained from rats dur

204 tch-seq(8) to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of mor

205 Here we have used biocytin staining, immunocytochemistry, and confocal mic

206 al slices using whole-cell current clamp and biocytin staining.

207 nsible for recycling the vitamin biotin from biocytin that is formed after the proteolytic degradatio

208 ns were labeled en mass with neurobiotin and biocytin through nerve roots, dye transfer was rarely ob

209 intercellular transfer of Lucifer yellow and biocytin throughout the 8-day culture period.

210 gnals with small extracellular injections of biocytin to assess quantitatively the specificity of hor

211 We used Co(++) or biocytin to backfill the entire pool of neurons that inn

212 We have used intracellular injection of biocytin to determine the morphology of cells with somas

213 Intracellular injection of biocytin to electrophysiologically identified neurones (

214 ade with pipette microelectrodes filled with biocytin to establish electrophysiological characteristi

215 direction, in which malonyl-CoA reacts with biocytin to form acetyl-CoA and carboxybiocytin.

216 We used biocytin to intracellularly label individual granule cel

217 ptic currents and intracellular filling with biocytin to quantify dendritic spines.

218 Each neuron was filled with biocytin to reveal its anatomy.

219 ssure (P) sensory neurons were injected with biocytin to reveal the extent of their sprouting 24 hour

220 P fluorescence were injected with the tracer biocytin to reveal their axonal projections.

221 We used the anterograde tracer biocytin to study experience-dependent changes in the sp

222 Application of biocytin to the nucleus preopticus periventricularis dem

223 amidal cells were recorded and injected with biocytin to visualize spines.

224 ase (WGA-HRP), the carbocyanine dye DiI, and biocytin) to determine the complete pattern of afferent

225 ed whether the clock regulates the extent of biocytin tracer coupling in the goldfish retina.

226 ere Thy-1-EGFP labeling in mouse, retrograde biocytin tracing in rat, cat, ferret, and macaque, SMI-3

227 This study was combined with biocytin tract tracing from the spinal cord to reveal de

228 In this case, 3-(N-maleimidylpropionyl)biocytin treatment of proteoliposomes containing His10V2

229 Biotinidase recycles the vitamin biotin from biocytin upon the degradation of the biotin-dependent ca

230 mulation were recorded, while simultaneously biocytin was injected for subsequent morphogenetic analy

231 At the end of recording biocytin was injected into the cell.

232 In this study, the intracellular tracer biocytin was used to identify the targets of the UM neur

233 Finally, a small molecule (biocytin) was encapsulated within the ferritin-PZn(2) ve

234 nd thick-tufted neurons, filled in vivo with biocytin, we were able to identify cell type-specific in

235 traneuronal injection of the neuronal tracer biocytin were integrated in a study of the functional ex

236 injections of fluorescent dextran amines or biocytin were made within subregions of HVC and pHVC to

237 relay, injections of the anterograde tracer biocytin were stereotaxically placed within the posterio

238 tracellular microelectrodes and injection of biocytin were used to study the actions of IL-1beta and

239 ification with Nalpha-(3-maleimidylpropionyl)biocytin, which attaches a biotin group to cysteine sulf

240 ant to labeling by 3-(N-maleimidylpropionyl)-biocytin while in membrane vesicle preparations.

241 The biocytin wide-field bipolar cell in rabbit retina has a

242 We conclude that the biocytin wide-field bipolar cell is an ON blue cone bipo