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1  the Pioneer inbred PHH5G is recalcitrant to biolistic and Agrobacterium transformation.
2 iled protocols for two different procedures, biolistic and electroporation, which have been used to t
3 lant transformation methods ( Agrobacterium, biolistics and protoplast fusion), insertion of DNA into
4 -null dorsal root ganglion (DRG) neurons via biolistics and were compared with the same construct exp
5                                            A biolistic approach was used to integrate two fusion gene
6           We evaluated particle bombardment (biolistics) as a strategy to introduce and express nucle
7  delivery: the PEG-mediated delivery and the biolistic bombardment based delivery, either of these me
8 in which it is not present in nature, by the biolistic bombardment of cultured immature embryos.
9                              In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion pr
10 ) and its effector function were verified by biolistic bombardment, ectopic expression and Hpa infect
11                                      We used biolistic combinatorial co-transformation (up to 20 tran
12 contamination, we explore the possibility of biolistic delivery as an alternate skin-based delivery r
13 mes as a preliminary investigation for using biolistic delivery as an alternative to syringe-and-need
14                                              Biolistic delivery is widely used for genetic transforma
15                                              Biolistic delivery of guide RNAs (as RNA molecules) dire
16    Here we present an optimized protocol for biolistic delivery of plasmid DNA into the epidermis of
17                               Here we report biolistic delivery of pre-assembled Cas9-gRNA ribonucleo
18  proteins from a single vector following its biolistic delivery or Agrobacterium-mediated genetic tra
19 tes in transgenic events generated from both biolistic delivery platforms.
20  a methodology to improve the consistency of biolistic delivery results by using a double-barrel devi
21                                          The biolistic delivery system is an essential tool in plant
22 yanine dyes, combined with particle-mediated biolistic delivery using a hand-held gene gun, allows no
23       Nuclear transformation methods include biolistic delivery, agitation with glass beads, and elec
24 arison and optimization of parameters in the biolistic delivery.
25 ry platforms: biolistic RNP/DNA co-delivery; biolistic DNA delivery; and Agrobacterium-mediated deliv
26 hloroplasts were successfully transformed by biolistic DNA-delivery and efficiently regenerated into
27    We incorporated these formulations into a biolistic format for gene gun by forming fireable dry bu
28                                    Using the biolistic gene gun, cDNAs encoding a dominant negative m
29                                    Using the biolistic gene gun, cDNAs encoding a mutant Kv4.2 alpha
30       One of the main transformation methods-biolistic gene transfer or Agrobacterium-mediated transf
31 ced into a homozygous rye inbred line by the biolistic gene transfer.
32                                    Using the Biolistics Gene Gun, cDNA constructs encoding a Kv1 subf
33                                    Using the Biolistics Gene Gun, cDNA constructs encoding either Kv2
34 e plasmid vaccines were delivered to mice by biolistic (gene gun) intradermal inoculation.
35 ed food production through Agrobacterium and biolistic genetic modifications for the development of '
36 days without using expensive equipment (e.g. biolistic gun or electroporation apparatus) or complicat
37 NP delivery into plant cells is the use of a biolistic gun.
38 r was bombarded into P. vittata spores using biolistics, in which putatively stable transgenic gameto
39  broadly applied to any target samples using biolistics, including animal cells and tissues.
40 s into the fundamental mechanisms underlying biolistic inefficiency and demonstrates a practical solu
41                                              Biolistic inoculation of cantaloupe seedlings with the M
42 Efficient protocols were established for the biolistic introduction of a construct containing a repor
43                         SAS was activated by biolistic introduction of promoterless constructs, indic
44                     In this current study, a biolistic leaf transformation assay has provided direct
45 -Nia2 DNA by the gene transfer method called biolistics led to localised acquired silencing (LAS) in
46          Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered
47                                              Biolistic loading of differentiated plant cells is easie
48 eveloped, including meristem editing through biolistic-mediated delivery and RNA virus-mediated deliv
49 s, and these particles were delivered by the biolistic method to plant cells harboring loxP sites fla
50  and to reach the desired tissue through the biolistic method.
51             Physical-based gene delivery via biolistic methods (such as the Helios gene gun) involve
52                                      We used biolistic methods to transfect neurons with gold 'bullet
53 into plastids of tobacco leaf tissues, using biolistic microprojectile bombardment, in an effort to d
54  of different plant species without external biolistic or chemical aid.
55 ypically achieved using Agrobacterium T-DNA, biolistics or by stably integrating nuclease-encoding ca
56 proteins into Ectocarpus cells, using either biolistics or microinjection as the delivery method.
57                                              Biolistic overexpression of M-CSFR on microglia endogeno
58 oration or into Anopheles gambiae embryos by biolistic particle bombardment.
59 f epidermal cells transiently transformed by biolistic particle delivery were used to confirm and ref
60 ximodistal respecification, we have used the biolistics (particle bombardment) technique to transfect
61 uptake in cells not initially transfected by biolistic penetration.
62 t, which are introduced into plastids by the biolistic process.
63 ce using three different delivery platforms: biolistic RNP/DNA co-delivery; biolistic DNA delivery; a
64  adult schistosomes further suggest that the biolistic strategy should be applicable to a variety of
65 RK1- and GIRK2-expressing plasmids using the biolistic technique.
66 estigate mechanisms of localization, we used biolistic techniques to express GFP-tagged PSD-95 (PSD-9
67  To overcome these problems, we have adapted biolistic techniques to load Ca2+-sensitive dyes into gu
68 method for introducing dyes into cells using biolistic techniques.
69                                        Using biolistics to label single cells on acute brain slices o
70           We have used particle bombardment (biolistics) to deliver replication-competent wheat dwarf
71 verexpression of Bcl-2 by means of transient biolistic transfection experiments of HUVE cells was suf
72                                              Biolistic transfection is a technique in which subcellul
73                                        Using biolistic transfection of individual RGCs and multielect
74                                 We have used biolistic transfection of organotypic slices of P12 cere
75      Here we provide a detailed protocol for biolistic transfection of plasmids into cultured human e
76                                              Biolistic transfection will facilitate experimental stra
77     Using organotypic hippocampal slices and biolistic transfection, we found the acid-sensing ion ch
78 models, and particle-mediated gene transfer (biolistics) transfection methods to identify critical DN
79 he C. lusitaniae CLS12 gene was disrupted by biolistic transformation and homologous recombination.
80                        Our results show that biolistic transformation can be used with transient assa
81                                              Biolistic transformation delivers nucleic acids into pla
82  generating stable transgenic tobacco lines, biolistic transformation of Antirrhinum petals and promo
83        We have developed a technique for the biolistic transformation of Arabidopsis thaliana root ha
84 nit of the chloroplast F1-ATPase was made by biolistic transformation of Chlamydomonas reinhardtii.
85    B chromosomes are readily fractionated by biolistic transformation of truncating plasmids.
86 e results suggest a model whereby successful biolistic transformation relies on a combination of end
87 air templates into maize immature embryos by biolistic transformation targeting five different genomi
88                                        Using biolistic transformation, we disrupted one of the two co
89 e then directly transformed into maize using biolistic transformation.
90 n gene was introduced in soybean by standard biolistic transformation.
91                                              Biolistic transient expression of UBA2-GFPs demonstrated
92                                              Biolistics using a promoter reporter showed that GA and