ライフサイエンスコーパス: biotinylation

1 cence-assisted cell sorting and cell surface biotinylation.

2 ssion of UT-A1, as evidenced by cell surface biotinylation.

3 carboxylase and catalyzes posttranslational biotinylation.

4 ecreased rate of endocytosis, as assessed by biotinylation.

5 yme with substrate limits post-translational biotinylation.

6 and ZIP14, as indicated by membrane protein biotinylation.

7 cell patch clamp recordings and cell surface biotinylation.

8 localized TRPC3, as determined using surface biotinylation.

9 ere further confirmed by basolateral surface biotinylation.

10 a67 fragment and BSA, and is subject to self-biotinylation.

11 or control, as determined by surface protein biotinylation.

12 al membrane CFTR as assessed by cell surface biotinylation.

13 e plasma membrane was tested by cell-surface biotinylation.

14 in WT but not in m1m2 cells, as measured by biotinylation.

15 ing under aging were validated by reversible biotinylation.

16 cB in a complex that is a poor substrate for biotinylation.

17 py, resonance energy transfer, and proximity biotinylation.

18 for stabilizing spirolactones for subsequent biotinylation.

19 says, gel filtration and proximity-dependent biotinylation.

20 ased on their cell-surface enzymatic fucosyl-biotinylation.

21 as was accessibility of the Cys residues to biotinylation.

22 the presence of MA made Env inaccessible to biotinylation.

23 Surface NHE3 was determined by cell surface biotinylation.

24 coupled with flow cytometry and cell surface biotinylation.

25 Cell surface biotinylation after mechanical injury indicates that pro

26 ne, as SpoVAEa was accessible to an external biotinylation agent in spores and SpoVAEa disappeared in

27 es of exposure to PregS, as shown by surface biotinylation and affinity purification.

28 Cell membrane proteins were enriched by biotinylation and avidin precipitation and analyzed by t

29 PM-coated electrode through further surface biotinylation and bridging avidin or NeutrAvidin.

30 es of pulse-chase labelling, domain-specific biotinylation and cell fractionation.

31 We present a workflow for biotinylation and characterization of recombinant antibo

32 Furthermore, the biotinylation and co-immunoprecipitation combined assays

33 ing, immunogold electron microscopy, surface biotinylation and computational modeling, we demonstrate

34 sent on the cell surface, demonstrated using biotinylation and confocal imaging, as well as in the cy

35 ated conclusion is supported by cell surface biotinylation and confocal microscopy of endogenous hCTR

36 ve mutant S138C hRFCs, combined with surface biotinylation and confocal microscopy, a dominant-negati

37 E3 distribution was assessed by cell-surface biotinylation and confocal microscopy.

38 ) were immobilized in the plasma membrane by biotinylation and cross-linking with avidin.

39 Using surface biotinylation and dual immunofluorescence staining in MY

40 and confocal microscopy coupled with surface biotinylation and electrophysiology suggest that changes

41 Surface biotinylation and endoglycosidase digestion experiments

42 Cell surface biotinylation and endoglycosidase H analysis revealed a

43 Cell surface biotinylation and immunoblotting with epitope-specific a

44 Cell surface biotinylation and immunofluorescence experiments in cell

45 Arterial biotinylation and immunofluorescence indicated that TRPP

46 In HTR cells, combined biotinylation and immunoprecipitation studies revealed p

47 In the present work we employ cell surface biotinylation and isotopic copper uptake studies of over

48 els of APP, as determined by surface protein biotinylation and live cell staining.

49 of ApoEr2, as determined by surface protein biotinylation and live cell surface staining.

50 resent here an in vitro method that combines biotinylation and mass spectrometry (MS) to identify sub

51 Here, we use proximity biotinylation and mass spectrometry to identify the AIS

52 Studies applying in vivo biotinylation and mathematical modeling showed that newb

53 Using biotinylation and membrane fractionation assays, prolong

54 Importantly, surface ZIP8 and ZIP14 biotinylation and Mn(2+)-uptake experiments together rev

55 anol, and surface receptors were isolated by biotinylation and P2 fractionation, whereas functional a

56 Cell surface biotinylation and selective inactivation of surface EGFR

57 Surface biotinylation and streptavidin pull-down of cells expres

58 Site-specific biotinylation and streptavidin staining take only a few

59 ealthy and CF donors was assessed by surface biotinylation and subsequent Western blot analysis.

60 Using plasma membrane biotinylation and super-resolution microscopy, we demons

61 cessibility technique combining cell-surface biotinylation and tandem-affinity purification to study

62 al membrane protein expression, we performed biotinylation and total internal reflection fluorescence

63 Using biotinylation and two-electrode voltage-clamp on Xenopus

64 units and their endocytosis were measured by biotinylation and Western blots.

65 rotein interactome on the basis of proximity biotinylation, and applied it to distinct chromatin modi

66 chemistry, single-particle tracking, surface biotinylation, and calcium imaging.

67 ical digestion, Cu(II)-mediated spirolactone biotinylation, and enrichment by avidin affinity chromat

68 e-cell patch clamp analysis, immunostaining, biotinylation, and immunoprecipitation methods to invest

69 e, a putative role for the enzyme in histone biotinylation, and its participation as a nuclear factor

70 were confirmed in human DAT-N2A cells using biotinylation, and similar effects were detected in rat

71 patch clamp electrophysiology, cell surface biotinylation, and total internal reflection fluorescenc

72 ium channel 1.2) channel expression, surface biotinylation, and Westerns were performed.

73 this study, we exploited the proximity-based biotinylation approach to identify binding partners of T

74 orientation using the chemical labeling and biotinylation approach, the "Cys-null" mutant of SNAT4 w

75 ing experiments with domain-specific surface biotinylation as well as immunocytochemical analysis of

76 trate the time scales required for efficient biotinylation as well as the hazards of overbiotinylatio

77 This was confirmed using a surface protein biotinylation assay and Brefeldin A disruption of the ro

78 A cell surface biotinylation assay and live cell confocal imaging studi

79 Finally, the in-cell biotinylation assay did not provide any evidence for the

80 Biotinylation assay further showed that POSH decreased s

81 ng, and surface expression was analyzed by a biotinylation assay in cRNA-injected Xenopus laevis oocy

82 o subapical intracellular compartment, and a biotinylation assay showed approximately 83% lower surfa

83 A biotinylation assay suggested that insulin and IGF1 inhi

84 rat ASIC3 (nmrASIC3) and used a cell-surface biotinylation assay to demonstrate that it traffics to t

85 and Fmr1 KO mice and immunocytochemistry and biotinylation assay to study related changes of 2-amino-

86 Using a reversible biotinylation assay, we quantified internalized hCTR1, w

87 hypothesis we developed a compartmentalized biotinylation assay, which we used to show that the C-te

88 hippocampal membranes was determined using a biotinylation assay.

89 MDA on TRPC6 was observed using cell surface biotinylation assays and also with whole-cell recordings

90 ar compartments, as assessed by cell-surface biotinylation assays and confocal microscopy.

91 Here, using surface-biotinylation assays and electrophysiology techniques, w

92 Using cell-surface biotinylation assays and highly inclined and laminated o

93 Membrane biotinylation assays and immunostaining showed that endo

94 In vivo biotinylation assays confirmed that nephrin expression d

95 ATPase and biotinylation assays in mpkCCD(c14) cells demonstrated t

96 Biotinylation assays revealed a significant increase in

97 Biotinylation assays showed reduced cell surface abundan

98 ernal Reflection Fluorescence microscopy and biotinylation assays showed that CASK silencing increase

99 studies using HC-3 binding and cell surface biotinylation assays showed that the PS-1 mutation inhib

100 og JHC1-64 and by reversible and pulse-chase biotinylation assays showing evidence for lysosomal degr

101 used both immunofluorescence microscopy and biotinylation assays to assess the trafficking of ClC-3.

102 Results of surface biotinylation assays using COS-7 cells suggest that Kv4.

103 Proximity biotinylation assays with LMP1 induced biotinylation of

104 ns on the cell surface were shown by surface biotinylation assays.

105 and total Slo1 as indicated by cell-surface biotinylation assays.

106 RPC3 membrane insertion, as shown by surface biotinylation assays.

107 ssor but also suggest that localized protein biotinylation at DNA damage sites is a useful strategy t

108 R118G) with RAD18 leads to localized protein biotinylation at DNA damage sites, allowing identificati

109 specificity compared with the commonly used biotinylation-based assays.

110 Biotinylation-based recycling and degradation studies in

111 uantitative imaging, fluorescent and surface biotinylation-based trafficking assays in cultured podoc

112 also detected using the proximity-dependent biotinylation (BioID) assay.

113 ress this gap, we used multiplexed proximity biotinylation (BioID) to generate an in situ, proximity-

114 Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium inter

115 coimmunoprecipitation (co-IP) and proximity biotinylation (BioID), to identify viral and cellular pr

116 After biotinylation by exposure to extracellular MTSEA-biotin

117 ety of recombinant proteins and demonstrated biotinylation by yBL at the N-terminus, C-terminus, and

118 One-minute proximity biotinylation captured candidate binding partners for hT

119 We performed biotinylation combined with glycocapture and high throug

120 Here, we utilize domain-selective surface biotinylation combined with stable isotope labeling with

121 was at least 3-fold better using C-terminal biotinylation compared with tagging at the N terminus us

122 P) sensor chips, we investigated a number of biotinylation conditions for target ligands.

123 Surface biotinylation confirmed the locus of RARalpha expression

124 Using cell surface protein biotinylation coupled with immunoblotting and immunofluo

125 Patch clamp and surface biotinylation data show reciprocal downregulation of eac

126 Using a novel application of surface biotinylation, data indicated that >95% of Ca(V)1.2 alph

127 Pulse labeling with [(35)S]methionine and biotinylation demonstrated that about 25% of newly synth

128 Cell surface biotinylation demonstrated that KCa2.3 was rapidly endoc

129 Membrane biotinylation demonstrated that nonfunctional mBest3 pro

130 Surface biotinylation demonstrated the loss of ENaC from the api

131 The position of Fab biotinylation dictates the geometry of multimer assembly

132 Biotinylation experiments also revealed an increase in s

133 Cell-surface protein biotinylation experiments and immunofluorescence studies

134 Biotinylation experiments and single-channel analysis re

135 thin endosomal compartments, whereas surface biotinylation experiments confirmed that phosphorylated

136 Immunofluorescence studies and surface biotinylation experiments demonstrated that the mutant p

137 tibody uptake experiments as well as surface biotinylation experiments demonstrated that the region b

138 Biotinylation experiments demonstrated that this result

139 Biotinylation experiments followed by immunostaining by

140 Biotinylation experiments in COS-7 cells show that altho

141 Indeed, pulse-chase biotinylation experiments in IECs lacking AnxA2 demonstr

142 supported by confocal imaging and reversible biotinylation experiments in transfected differentiated

143 Cell surface biotinylation experiments on KChIP4a indicate that the N

144 Biotinylation experiments revealed that the rapid increa

145 Western blotting and surface biotinylation experiments show that loss of myoferlin re

146 Cell surface biotinylation experiments showed approximately 45% reduc

147 Surface biotinylation experiments suggest that the non-wild-type

148 A series of cell-surface biotinylation experiments suggests that anterograde traf

149 Combining with oocyte surface biotinylation experiments, we demonstrated that RACK1 in

150 in mouse cortical cultures as determined by biotinylation experiments.

151 icing assays, deletion mapping, cell-surface biotinylation, expression studies and molecular modellin

152 Chemical biotinylation followed by enrichment and mass spectromet

153 We used proximity biotinylation followed by mass spectrometry and direct b

154 Maleimide-PEO2-biotin precipitated (surface biotinylation followed by Western blotting) and reduced

155 n-protein interaction detection by proximity biotinylation has the advantages of low background, high

156 Both real-time imaging and surface biotinylation have demonstrated internalization of alpha

157 Metabolic labeling, cell surface biotinylation, immobilized lectins, and confocal immunof

158 Importantly, cell surface biotinylation, immunofluorescence and down-regulation ex

159 Biotinylation, immunofluorescence resonance energy trans

160 Using surface biotinylation, immunohistochemistry, electron microscopy

161 rotein partners, we utilized BioID proximity biotinylation in combination with stable isotopic labeli

162 munolocalization in oocytes and cell surface biotinylation in HEK cells indicated that the WNK-mediat

163 Cell-surface biotinylation in hepatocytes confirmed that ABCC6 is int

164 ptimized a procedure for proximity-dependent biotinylation in live tissue using the APEX enzyme to in

165 e and function of Arc1p can be modulated via biotinylation in response to temperature changes.

166 residues of beta protein are protected from biotinylation in the DNA complex, whereas none of the ly

167 approach based on cell type-specific in vivo biotinylation in zebrafish to analyse the initial respon

168 ther, these results indicate that C-terminal biotinylation is a promising tagging strategy for develo

169 rent molecular weight (MW), 66 kDa], surface biotinylation labeled an ERalpha-immunoreactive protein

170 m 30 to 37 degrees C drastically reduced its biotinylation level.

171 escribe an NBS specific UV photocrosslinking biotinylation method (UV-NBS(Biotin)) for the oriented i

172 ndent uptake of (14)C-TC, and a cell surface biotinylation method was used to determine Ntcp and Mrp2

173 e as well as the less commonly used carboxyl biotinylation methods.

174 ts but are not biotinylated due to a mutated biotinylation motif.

175 gulators of ACCase after initial loss of the biotinylation motif.

176 To address this issue, we have used MTSEA-biotinylation (N-Biotinoylaminoethyl methanethiosulfonat

177 Likewise, biotinylation occurred after treatment with beta-mercapt

178 ated; however, in the absence of either one, biotinylation occurred.

179 Apical biotinylation of (MPK)CCD(14) cells confirmed the loss o

180 interactions based on interaction-dependent biotinylation of a peptide by E. coli biotin ligase, to

181 id metabolism through the post-translational biotinylation of acyl coenzyme A carboxylases.

182 Here we used a proteomic screen with in vivo biotinylation of AID to identify the splicing regulator

183 an efficient strategy for the site-selective biotinylation of an antibody as well as for the condensa

184 s interact, BirA will catalyze site-specific biotinylation of AP, which can be detected by streptavid

185 Nevertheless, biotinylation of Arc1p was temperature dependent; raisin

186 Biotinylation of cell surface proteins revealed decrease

187 n internalization at the BTB was assessed by biotinylation of cell surface proteins, to be followed b

188 yonic stem (mES) cells, we have used in vivo biotinylation of critical transcription factors for affi

189 e establishment of an assay that is based on biotinylation of effectors in the host cytoplasm as hall

190 apid and simple method that combines in vivo biotinylation of engineered host-specific bacteriophage

191 Biotinylation of free Ku revealed several reactive lysin

192 Biotinylation of histones plays crucial roles in the rep

193 Moreover, biotinylation of intestinal luminal proteins from mice f

194 Metabolic biotinylation of intracellular and secreted proteins as

195 Biotinylation of MV4-11 cell surface proteins followed b

196 on activation, the APEX enzyme catalyzes the biotinylation of neighboring endogenous proteins that ca

197 The biotinylation of PCFT was further confirmed by blocking

198 onclusions are based on studies in which (i) biotinylation of peritoneal macrophages showed that endo

199 fic cellular RNAs for RNA-centered proximity biotinylation of protein interaction partners.

200 Here, we show that metabolic biotinylation of proteins fused to the bacterial biotin

201 BioID features proximity-dependent biotinylation of proteins that are near-neighbors of the

202 Using cell-surface protein biotinylation of rat cerebellar slices, we found secreti

203 Biotinylation of surface proteins demonstrates that cell

204 bacterium's DC surface proteome by selective biotinylation of surface proteins, NeutrAvidin affinity

205 ophysiology, immunofluorescent staining, and biotinylation of surface receptors.

206 In this study, the selective biotinylation of surface-exposed proteins, streptavidin

207 By contrast, biotinylation of the acetyl-CoA carboxylase 1 and 2 (ACC

208 imity biotinylation assays with LMP1 induced biotinylation of the actin-associated proteins, which we

209 The results demonstrate that biotinylation of the BCCP fragments of the mitochondrial

210 -AMP from biotin and ATP, and the succeeding biotinylation of the biotin carboxyl carrier protein.

211 Simple biotinylation of the DNA template allows for labeling of

212 inylation of TMD2 mutants G93C and F94C, and biotinylation of these residues inhibited methotrexate t

213 Pemetrexed pretreatment inhibited biotinylation of TMD2 mutants G93C and F94C, and biotiny

214 OMP-1B was undetectable either by surface biotinylation or by Western blotting of the whole bacter

215 a membrane compared to a sulfo-NHS-activated biotinylation or two-step SEEL.

216 the predicted C-terminal SAP domain of Ku70, biotinylation patterns were observed which suggest a str

217 ssociation rates with HCS in order to ensure biotinylation prior to mitochondrial import.

218 omponents, such as Tris and glycerol, on the biotinylation process.

219 r functional cluster of TMEM16K in proximity biotinylation proteomics analyses.

220 Interaction and proximity biotinylation proteomics identified a cohort of autophag

221 Using a proximity-dependent biotinylation proteomics strategy, we identify 200 putat

222 Here, we used proximity biotinylation proteomics to dissect the different protei

223 ) as the ligand of GPR56 through an in vitro biotinylation/proteomics approach.

224 We also demonstrate that the biotinylation range of BioID2 can be considerably modula

225 e two recombinant BirA proteins catalyze the biotinylation reaction of the acceptor biotin carboxyl c

226 abeled with an impermeant, cysteine-specific biotinylation reagent (MTSEA-biotin) with or without per

227 Access to a membrane-impermeant biotinylation reagent as well as protease sensitivity wa

228 gh these levels were not increased by higher biotinylation reagent concentrations or longer reaction

229 proteins, a cell-impermeant, thiol-reactive biotinylation reagent was used to label and subsequently

230 could be labeled with a membrane-impermeant biotinylation reagent, indicating surface exposure.

231 By using bulky biotinylation reagents on intact cells, we showed that t

232 hly efficient, identifying specific sites of biotinylation remains challenging.

233 Cell surface biotinylation results indicated that Orai1 channels in t

234 We used the in vivo biotinylation retro-translocation assay in mammalian cel

235 sistent with a reduction in current density, biotinylation revealed a significant reduction in surfac

236 Surface biotinylation revealed rapid loss of PAM-1/H3A, with no

237 Biotinylation reveals a 32-kDa, covalently cross-linked

238 Cell surface biotinylation reveals that plasma membrane GLUT1 levels

239 visualization, we developed a RNA proximity biotinylation (RNA-BioID) technique by tethering biotin

240 Cell surface biotinylation, sensitivity to glycosidases, and fluoresc

241 Surface biotinylation showed that dobutamine did not affect plas

242 A recombinant form of CPS containing a biotinylation site from an Escherichia coli protein is a

243 crease of more than 30-fold in the number of biotinylation sites identified compared to streptavidin-

244 ent and mass spectrometry yielded over 1,600 biotinylation sites on hundreds of proteins, an increase

245 Thus, we used a novel biotinylation strategy to verify protein localization, a

246 Using a biotinylation strategy, it was found that surface expres

247 Plasma membrane biotinylation studies and confocal microscopic examinati

248 Next, we performed surface biotinylation studies in acutely dissociated hippocampal

249 However, surface biotinylation studies in HIT-T15 cells indicate that Rem

250 l current density, immunohistochemistry, and biotinylation studies in isolated hearts and cardiomyocy

251 Ex vivo biotinylation studies in mouse striatal slices demonstra

252 Fluorescence flow cytometry and biotinylation studies revealed a rapid increase in DAT c

253 ax) of the transporter; whereas cell surface biotinylation studies revealed no alteration in the cell

254 Rather, cell surface biotinylation studies showed an increased number of CaV2

255 Cell surface biotinylation studies showed that both GLYX-13 and ketam

256 Using cell surface biotinylation studies, we detected endogenous sgk1 at th

257 neurons using the pHluorin-GluR1 imaging and biotinylation studies, we observed that prolonged morphi

258 BioTag, a 23-amino-acid peptide serving as a biotinylation substrate for BirA, in vivo in worms.

259 otin acceptor substrates and the kinetics of biotinylation suggests that mitochondrial carboxylase se

260 ee procedures will be described: (i) in vivo biotinylation system setup in mES cells; (ii) affinity p

261 oblem, we report here the use of a sensitive biotinylation system to probe the dislocation of major h

262 gamma containing a C-terminal biotinylation tag (gamma-C(tag)) was provided in trans a

263 By using an in vivo biotinylation tagging approach followed by liquid chroma

264 Using biotinylation tagging technology and high-throughput seq

265 proach using hexahistidine and BirA-specific biotinylation tags for isolating translocated effector c

266 protein denaturation using polyhistidine and biotinylation tags.

267 on of CD4 and BST-2/Tetherin using our novel biotinylation technique in living cells to determine ER-

268 channel in combination with fluorescence and biotinylation techniques in both human embryonic kidney

269 a2.3 using a combination of fluorescence and biotinylation techniques.

270 D-based PL provides more efficient levels of biotinylation than BioID and BioID2 in plants.

271 ass spectrometry with selective cell surface biotinylation to characterise the classical monocyte sur

272 We used surface biotinylation to demonstrate that ERalpha has an extrace

273 transport characterization and cell-surface biotinylation to examine the residues involved in inhibi

274 In the present study, we used surface biotinylation to identify and study the estradiol regula

275 nd combined this technique with cell surface biotinylation to identify surface-exposed proteins of a

276 We use proximity biotinylation to identify the AC9 interaction network, w

277 In this study, we used selective biotinylation to label proteins localized to the surface

278 cipitation and live cell proximity-dependent biotinylation to monitor interactions between Glrx3, Bol

279 We used cell surface biotinylation to monitor NPC1L1-GFP endocytosis and show

280 We used reversible biotinylation to monitor the GPCR trafficking using SMCs

281 method along with surface and site-specific biotinylation to probe TM6 for aqueous accessibility and

282 K293T cells we used fluorescence and surface biotinylation to quantify Fpn membrane occupancy and als

283 imaging, co-immunoprecipitation, and surface biotinylation to study the functional consequences of a

284 Selectivity in HCS-catalyzed biotinylation to the carboxylases was investigated in si

285 We used proximity biotinylation to uncover new constituents of the inner n

286 Our results highlight the power of proximity biotinylation to yield insights into the molecular compo

287 Third, by cell surface biotinylation, trafficking of NHE3 was examined in short

288 ed GLIB (glucosylation, periodate oxidation, biotinylation) uses a combination of enzymatic and chemi

289 for photolabeling, and an alkyne moiety for biotinylation via "click chemistry".

290 ferential detergent fractionation or surface biotinylation was used directly without immunoprecipitat

291 ular N-terminal GluA1 antibody or by surface biotinylation, was impaired following knockdown of CaMKK

292 Using peroxidase-mediated proximity biotinylation, we captured and identified endogenous pro

293 Using APEX2 proximity biotinylation, we demonstrated that Rbd2 binds the AAA-A

294 tative protein mass spectrometry and surface biotinylation, we identified 100 proteins that showed si

295 Following surface biotinylation, Western blot analysis revealed full-lengt

296 were targeted to the cell surface by surface biotinylation/Western blots and confocal microscopy and

297 GLIB (glucosylation, periodate oxidation and biotinylation), which combines several enzymatic and che

298 Here, we visualized IPR by in vivo biotinylation, which revealed that the major IPR is a gr

299 d regulators by performing in vivo proximity biotinylation with mitochondrially-localized forms of th

300 alyzed, spatially restricted in situ protein biotinylation with RNA-protein chemical crosslinking.