ライフサイエンスコーパス: bipolar spindle

1 ary for the formation and maintenance of the bipolar spindle.

2 pha-tubulin or developed an abnormally small bipolar spindle.

3 te in an antiparallel orientation, forming a bipolar spindle.

4 n, although it was dispensable for forming a bipolar spindle.

5 opposite spindle to provide stability to the bipolar spindle.

6 man kinesin involved in the formation of the bipolar spindle.

7 mic instability, bundle, and organize into a bipolar spindle.

8 ration is a prerequisite for positioning the bipolar spindle.

9 s on the organization of microtubules into a bipolar spindle.

10 ome segregation depends on the assembly of a bipolar spindle.

11 ntial in many species for the formation of a bipolar spindle.

12 ) and occurs before the establishment of the bipolar spindle.

13 y towards the poles following formation of a bipolar spindle.

14 ion, which in turn affect the formation of a bipolar spindle.

15 le proteins change their distribution in the bipolar spindle.

16 est at metaphase of meiosis II with a normal bipolar spindle.

17 cell cycle to ensure development of a normal bipolar spindle.

18 values cause a monaster to form instead of a bipolar spindle.

19 omplex that cannot establish or maintain the bipolar spindle.

20 uclear envelope breakdown and formation of a bipolar spindle.

21 or the role of centrosomes in organizing the bipolar spindle.

22 ys that collaborate in the organization of a bipolar spindle.

23 ng and assembly of these microtubules into a bipolar spindle.

24 ides a force required for the formation of a bipolar spindle.

25 that are required for the establishment of a bipolar spindle.

26 les to the forces involved in formation of a bipolar spindle.

27 nd stabilize microtubules in order to form a bipolar spindle.

28 cultured mammalian cells does not require a bipolar spindle.

29 f chromosomes joined by a chiasma requires a bipolar spindle.

30 in, NCD, in tapering the microtubules into a bipolar spindle.

31 uring prophase these cells form a functional bipolar spindle.

32 ssential for the structural integrity of the bipolar spindle.

33 hin the nucleus to assemble and elongate the bipolar spindle.

34 centrosomes and thereby to the assembly of a bipolar spindle.

35 es progressively connects chromosomes to the bipolar spindle.

36 ive at coalescing extra spindle poles into a bipolar spindle.

37 tubules and the ability to form a simplified bipolar spindle.

38 clustering, is essential for formation of a bipolar spindle.

39 uired for the formation and maintenance of a bipolar spindle.

40 hromosome segregation is accomplished by the bipolar spindle.

41 of microtubule length within the assembling bipolar spindle.

42 osomes to separate and form the poles of the bipolar spindle.

43 in, and, ultimately, for formation of normal bipolar spindles.

44 ntil all sister kinetochores are attached to bipolar spindles.

45 to facilitate the transition from asters to bipolar spindles.

46 o-astral and monopolar structures instead of bipolar spindles.

47 protein that is required for organization of bipolar spindles.

48 ing spindle assembly blocks the formation of bipolar spindles.

49 rphase, enter mitosis normally, and assemble bipolar spindles.

50 rrest in G2 of the first cycle with complete bipolar spindles.

51 scapine arrest at mitosis with nearly normal bipolar spindles.

52 entrioles/centrosomes and the maintenance of bipolar spindles.

53 insert into the NM at meiosis I and nucleate bipolar spindles.

54 centrosomes could compromise the assembly of bipolar spindles.

55 r Ran on chromosomes, established functional bipolar spindles.

56 sis, centrosome maturation, and formation of bipolar spindles.

57 f mitotic cells with aligned chromosomes and bipolar spindles after dosing.

58 FZR1 controls the timing of assembly of the bipolar spindle and in so doing the timing of SAC satisf

59 ing cell division, the proper formation of a bipolar spindle and its function to segregate chromosome

60 a kinesin implicated in the formation of the bipolar spindle and its movement prior to and during ana

61 Plant cells assemble the bipolar spindle and phragmoplast microtubule (MT) arrays

62 t mediates Ran-GTP-dependent assembly of the bipolar spindle and promotes chromosome congression and

63 s during metaphase, after the formation of a bipolar spindle and the destruction of cyclin A, and it

64 ntrosome clustering mechanisms to assemble a bipolar spindle and to divide in a bipolar fashion.

65 he ability of A-549 and Calu-1 cells to form bipolar spindles and caused formation of monoasteral spi

66 meiosis I which results in a failure to form bipolar spindles and divide nuclei.

67 aturation, for assembly and maintenance of a bipolar spindle, and for proper chromosome segregation d

68 oteins are required for the assembly of this bipolar spindle, and while the meiotic spindle lacks tra

69 tic events such as assembly and stability of bipolar spindles, and faithful chromosome segregation in

70 s results in delayed spindle assembly, fewer bipolar spindles, and the appearance of aberrant microtu

71 itosis by contributing to the formation of a bipolar spindle apparatus.

72 ends on the formation of a microtubule-based bipolar spindle apparatus.

73 nds on formation of a microtubule (MT)-based bipolar spindle apparatus.

74 urthermore, this elongation does not require bipolar spindle architecture or dynamic microtubules.

75 ngly, when just one centrosome is destroyed, bipolar spindles are also formed that contain one centro

76 Bipolar spindles assemble in the absence of centrosomes

77 ocyte meiotic cell division in many animals, bipolar spindles assemble in the absence of centrosomes,

78 without centrioles exhibited both a delay in bipolar spindle assembly and a high rate of chromosomal

79 osomal component of the spindle required for bipolar spindle assembly and function.

80 f microtubule-based motors, is essential for bipolar spindle assembly and maintenance during mitosis,

81 ration, indicating a role of SUN proteins in bipolar spindle assembly and mitotic progression.

82 tein phosphatase PP2A(Cdc55) activity blocks bipolar spindle assembly and nuclear divisions.

83 within the spindle midzone to play roles in bipolar spindle assembly and proper chromosome distribut

84 ked the ability of excess XCTK2 to stimulate bipolar spindle assembly and resulted in XCTK2-mediated

85 required for microtubule polymerization and bipolar spindle assembly around chromatin beads.

86 l cell cycle events of nuclear formation and bipolar spindle assembly around exogenously added sperm

87 yeast cells in mitosis at a stage following bipolar spindle assembly but prior to anaphase spindle e

88 Like Kif2a-deficient cells, bipolar spindle assembly can be restored to Kif2b-defici

89 drives efficient chromosome segregation and bipolar spindle assembly during mitosis.

90 s: first promoting centrosome separation and bipolar spindle assembly during prophase/prometaphase, a

91 eby paternal ZYG-1 regulates duplication and bipolar spindle assembly during the first cell cycle, an

92 re, this mechanism is strong enough to drive bipolar spindle assembly even in the presence of a singl

93 Bipolar spindle assembly in CDH1-m11 cells is strikingly

94 tides destabilizes microtubules and inhibits bipolar spindle assembly in HeLa cells.

95 the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells.

96 ule sliding generated by Eg5 activity during bipolar spindle assembly in mammalian cells is regulated

97 spindle, which was subsequently required for bipolar spindle assembly in S phase.

98 microtubule-associated proteins required for bipolar spindle assembly in the absence of the centrosom

99 ads , AurA-coated beads increase the rate of bipolar spindle assembly in the presence of RanGTP, and

100 he microtubule assembly pathways compromises bipolar spindle assembly in tissue culture cells but not

101 ement in balancing Eg5-induced forces during bipolar spindle assembly in vitro and in vivo, and show

102 Bipolar spindle assembly is a critical control point for

103 Bipolar spindle assembly is critical for achieving accur

104 rosome, but, in its absence, the fidelity of bipolar spindle assembly is highly compromised.

105 le assembly pathways are integrated to drive bipolar spindle assembly is poorly understood.

106 edly, we found both microtubule assembly and bipolar spindle assembly required glycogen, which acted

107 Bipolar spindle assembly requires a balance of forces wh

108 Bipolar spindle assembly was restored in cells lacking K

109 rotation of the centrosome/nucleus complex, bipolar spindle assembly, anaphase chromosome segregatio

110 oles of mitotic spindles and is required for bipolar spindle assembly, but its molecular function in

111 ocity approximately 20%, but does not hinder bipolar spindle assembly, chromosome alignment, or mitot

112 microtubules and are instrumental in mitotic bipolar spindle assembly, ensuring orderly cell cycle pr

113 We show that four kinesins are involved in bipolar spindle assembly, four kinesins are involved in

114 /Thr-17-21 cluster was the most critical for bipolar spindle assembly, whereas other phospho-deficien

115 iated proteins (MAPs) is required for proper bipolar spindle assembly, yet the precise mechanisms by

116 nd antagonizes MCAK activity, thus promoting bipolar spindle assembly.

117 Hice1 in a spatiotemporal manner for proper bipolar spindle assembly.

118 NP-O) and Fta1R(CENP-L)) causes a failure in bipolar spindle assembly.

119 e midzone-associated protein is required for bipolar spindle assembly.

120 ts suggest a mechanism for how Kif15 rescues bipolar spindle assembly.

121 o separate centrosomes, thus ensuring robust bipolar spindle assembly.

122 leus at the onset of mitosis is critical for bipolar spindle assembly.

123 ting pericentriolar material cohesion during bipolar spindle assembly.

124 ng to help focus spindle poles for efficient bipolar spindle assembly.

125 hat underlies both centrosome maturation and bipolar spindle assembly.

126 (MT) motor, pushes centrosomes apart during bipolar spindle assembly; its suppression results in mon

127 enerate antagonistic forces during mammalian bipolar spindle assembly; what remains unknown, however,

128 re-spindle misattachments and an increase in bipolar spindles associated with ectopic asters.

129 rosome during interphase, the formation of a bipolar spindle at mitosis and cytokinesis.

130 d with the control microspores, which formed bipolar spindles at the cell periphery, the mutant cells

131 ng cell division, microtubules that form the bipolar spindle attach to and pull on paired chromosome

132 ossover recombination into one that promotes bipolar spindle attachment and localized cohesion loss.

133 is centromere pairing mediates the meiosis I bipolar spindle attachment of nonexchange chromosome pai

134 s kinetochore structure, causes a failure of bipolar spindle attachment, and results in chromosome no

135 s the efficiency with which chromosomes make bipolar spindle attachments and regulates kinetochore ac

136 sts that they are unable to establish stable bipolar spindle attachments, presumably due to the inabi

137 s are connected to the opposite poles of the bipolar spindle ("bioriented").

138 show that the majority of cells establish a bipolar spindle but have defects in spindle orientation.

139 d-sensitive mitotic arrest with an elongated bipolar spindle but impaired anaphase A.

140 In the absence of AurA, cells form bipolar spindles but fail to properly align their chromo

141 aphase spermatocytes were characterized with bipolar spindles, but chromosomes radiated away from the

142 ts enter mitosis at the normal time and form bipolar spindles, but fail chromosome alignment at the m

143 its ability to cluster centrosomes and form bipolar spindles, but it is not required for division in

144 Duo1p and Dam1p are not required to assemble bipolar spindles, but they are required to maintain meta

145 the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, i

146 Kinesin-5s help assemble the bipolar spindle by crosslinking and sliding apart antipa

147 study reveals that mouse oocytes assemble a bipolar spindle by fragmenting multiple acentriolar micr

148 KCBP promotes the formation of a converging bipolar spindle by sliding and bundling microtubules.

149 ng microtubules, but cells eventually formed bipolar spindles by an acentrosomal pole-focusing mechan

150 ticentrosomal, yet they are able to assemble bipolar spindles by clustering centrosomes into two spin

151 Hsp72, is required for assembly of a robust bipolar spindle capable of efficient chromosome congress

152 noscapine-arrested cells have nearly normal bipolar spindles, cells arrested by 5-Br-nosc and Rd 5-B

153 Here we report that the bipolar spindle changes its molecular architecture durin

154 Time-lapse movies of GFP-labeled mono- and bipolar spindles demonstrate that KLP-19 generates a for

155 , the proper assembly of a microtubule-based bipolar spindle depends on signals from chromatin.

156 Imaging bipolar spindles disassembling in the presence of monast

157 e is important for spindle assembly, because bipolar spindles do not form in cells lacking centrosome

158 ome segregation depends on the assembly of a bipolar spindle driven by the timely separation of the t

159 es novel insight into how oocytes maintain a bipolar spindle during metaphase arrest.

160 inesin-5/Klp61F is crucial for maintaining a bipolar spindle during metaphase I arrest.

161 icates during the cell cycle and assembles a bipolar spindle during mitosis to capture and segregate

162 Kinesin-5 is required for forming the bipolar spindle during mitosis.

163 l role in orchestrating the formation of the bipolar spindle during mitosis.

164 r protein, is essential for the formation of bipolar spindles during mitosis.

165 osomes clustered into two poles whose pseudo-bipolar spindles exhibit reduced fidelity of chromosome

166 When Fin1 is mislocalized, bipolar spindles fail to assemble but the spindle checkp

167 chromosomes are attached and oriented on the bipolar spindle for subsequent segregation at anaphase.

168 In the oocytes of many species, bipolar spindles form in the absence of centrosomes.

169 i-treated cells, we show that poorly focused bipolar spindles form through the self-organization of m

170 pair of centrioles separate, and functional bipolar spindles form with only one centriole at each sp

171 y (SPB) once per cell cycle is essential for bipolar spindle formation and accurate chromosome segreg

172 mitotic activity as evidenced by blockade of bipolar spindle formation and appearance of monoasters.

173 entrosomes play a pivotal role in regulating bipolar spindle formation and cell division.

174 ough mitosis and tumor growth is by blocking bipolar spindle formation and chromosome alignment.

175 is, Kif11, a kinesin motor protein, promotes bipolar spindle formation and chromosome movement, and d

176 importance of alpha-N-methylation for normal bipolar spindle formation and chromosome segregation.

177 nterfering RNA (siRNA) and showed defects in bipolar spindle formation and cytokinesis, growth inhibi

178 plk1 Delta N resulted in repeated cycles of bipolar spindle formation and disruption, suggestive of

179 MT-based MT nucleation to accomplish normal bipolar spindle formation and mitotic progression.

180 dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division.

181 ells at mitosis, likely due to the defect of bipolar spindle formation and subsequent activation of t

182 Centrosome separation, critical for bipolar spindle formation and subsequent chromosome segr

183 Centrosome separation is critical for bipolar spindle formation and the accurate segregation o

184 e extracts, Xkid and Xklp1 are essential for bipolar spindle formation but the functions of the human

185 omere positioning, microtubule dynamics, and bipolar spindle formation can all contribute to chromoso

186 he BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell divisio

187 kinase required for centrosome splitting and bipolar spindle formation during mitosis.

188 and Alp14/Dis1 play a collaborative role in bipolar spindle formation during prometaphase through pr

189 ator of centrosome duplication, required for bipolar spindle formation in HeLa human carcinoma cells

190 acentrosomal poles all contribute to robust bipolar spindle formation in meiotic extracts.

191 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for pr

192 hat lead to timely centrosome separation and bipolar spindle formation in mitosis.

193 omal microtubule assembly pathway and favors bipolar spindle formation in most animal cells in which

194 on in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.

195 ucleation plays an important role for proper bipolar spindle formation in various eukaryotic organism

196 d is essential for centrosome separation and bipolar spindle formation in vitro and in vivo.

197 Kif15-dependent bipolar spindle formation in vivo requires the C-termina

198 Monastrol also inhibits bipolar spindle formation in Xenopus egg extracts.

199 Bipolar spindle formation in yeast requires insertion of

200 Faithful chromosome segregation with bipolar spindle formation is critical for the maintenanc

201 Bipolar spindle formation is essential for the accurate

202 Bipolar spindle formation is pivotal for accurate segreg

203 Neither bipolar spindle formation nor centrosome functions were

204 to the spindles, thus resulting in improper bipolar spindle formation that ultimately leads to mitot

205 normal for assembly of the Ndc80 complex and bipolar spindle formation yet defective in proper end-on

206 ediate multiple mitotic processes, including bipolar spindle formation, activation of Cdc25C, actin r

207 omplex implicated in kinetochore attachment, bipolar spindle formation, and cytokinesis.

208 les, promotes microtubule polymerization and bipolar spindle formation, and decreases the turnover ra

209 or kinesin-5-mediated centrosome separation, bipolar spindle formation, and equal centrosome/centriol

210 e it regulates mitotic microtubule dynamics, bipolar spindle formation, and subsequent chromosome seg

211 tion of the PBD function results in improper bipolar spindle formation, chromosome missegregation, an

212 at Eg5 inhibition led to either monopolar or bipolar spindle formation, depending on whether centroso

213 rise from defects in centrosome duplication, bipolar spindle formation, kinetochore-microtubule attac

214 et of FTIs affecting centrosome position and bipolar spindle formation, likely explaining some of the

215 to allow stable K-MT attachments only after bipolar spindle formation, thus preventing attachment er

216 f Plk1 including (from low to high activity) bipolar spindle formation, timely mitotic entry, and for

217 ation site (S719), which positively enhances bipolar spindle formation.

218 and mitotic arrest with a profound defect in bipolar spindle formation.

219 ATPase activity and plays essential roles in bipolar spindle formation.

220 Plx1 functions on the centrosome to promote bipolar spindle formation.

221 lp14 and Dis1 share an essential function in bipolar spindle formation.

222 ed assembly of microtubules is essential for bipolar spindle formation.

223 lyses have revealed that sub is required for bipolar spindle formation.

224 and Cls1 activity are sufficient for initial bipolar spindle formation.

225 lp14 mutant fails to progress towards normal bipolar spindle formation.

226 ears to be defective in nuclear migration or bipolar spindle formation.

227 es centrosome separation and, in some cases, bipolar spindle formation.

228 t not cin8-3 cells, were greatly impaired in bipolar spindle forming ability.

229 The bipolar spindle forms without centrosomes naturally in f

230 at metaphase I, unlike the typical fusiform bipolar spindle found in the wild-type metaphase I cells

231 cell-cycle regulation that is independent of bipolar spindle function.

232 Bipolar spindle fusion was blocked when cytoplasmic dyne

233 ssential role in mitosis by establishing the bipolar spindle, has proven to be an interesting drug ta

234 ions have to be met to robustly assemble the bipolar spindle in a multicentrosomal cell: 1) the stren

235 Establishing the bipolar spindle in mammalian oocytes after their prolong

236 erlies its role in supporting formation of a bipolar spindle in mitosis.

237 om cytoplasmic transport to formation of the bipolar spindle in mitosis.

238 spindle pole separation and the assembly of bipolar spindle in the absence of molecular motors.

239 cross-linkers, fission yeast can assemble a bipolar spindle in the absence of motor proteins.

240 Collapse of bipolar spindles in MCAK-deficient cells is driven by po

241 aughter cells could not assemble functional, bipolar spindles in the ensuing mitosis.

242 phatase resulted in the rapid disassembly of bipolar spindles into large asters.

243 Chromosome alignment in the middle of the bipolar spindle is a hallmark of metazoan cell divisions

244 Like other cellular organelles, the bipolar spindle is a structure of well-defined size and

245 tic spindle, it remains unclear how a stable bipolar spindle is established.

246 taphase/anaphase transition to ensure that a bipolar spindle is formed and that all the chromosomes a

247 As a bipolar spindle is formed, the median MT length increase

248 nesis of the microtubule cytoskeleton into a bipolar spindle is required for the faithful transmissio

249 In the mipAD123/klpA1 strain, formation of bipolar spindles is more strongly inhibited than in the

250 ly focused, diamond-shaped appearance of the bipolar spindle, K fibers need to be interconnected with

251 ntrast, and differing from previous reports, bipolar spindle length is relatively insensitive to incr

252 py-number plasmid R1 involves formation of a bipolar spindle made of left-handed double-helical actin

253 hout Eg5, whereas stabilizing K-MTs improves bipolar spindle maintenance without Eg5.

254 hed kinetochore-MTs (K-MTs) is important for bipolar spindle maintenance without Eg5.

255 Bipolar spindles must separate chromosomes by the approp

256 implicated in the formation and function of bipolar spindles on the basis of their respective locali

257 5 activity is dispensable for maintenance of bipolar spindles once they are formed [3, 4], suggesting

258 at the nuclear envelope before NEBD, and the bipolar spindle only emerged clearly after NEBD.

259 bl-deficient testes exhibit abnormalities in bipolar spindle organization, chromosome segregation, an

260 of p53 is not activated by abnormalities in bipolar spindle organization, chromosome segregation, ce

261 ding modules are required to rescue focused, bipolar spindle organization.

262 As the two centrosomes split to assemble the bipolar spindle, predominantly the old centrosome migrat

263 We propose that the bipolar spindle propagates its own architecture by stimu

264 Under bipolar spindle pulling, the distorted centromeres are p

265 aberrant mitosis despite normal assembly of bipolar spindles, resulting in either apoptosis or forma

266 ation favors the establishment of an initial bipolar spindle scaffold, facilitating chromosome captur

267 von Economo neurons (VENs) are bipolar, spindle-shaped neurons restricted to layer 5 of

268 ring cytokinesis in cells with monopolar and bipolar spindles shows that a subpopulation of stable mi

269 nical centrosomes but are still able to form bipolar spindles, starting from an initial ball that sel

270 shows that when sliding is inhibited, short bipolar spindles still form, and if clustering is enhanc

271 upporting the formation of half-spindles and bipolar spindle structures around unreplicated chromosom

272 mouse oocytes, formation of the acentrosomal bipolar spindle takes 3-4 h, and stabilization of K-MT a

273 rotubule (MT) cytoskeleton rearranges into a bipolar spindle that drives chromosome segregation.

274 at mitosis depends on a correctly assembled bipolar spindle that exerts balanced forces on each sist

275 protein capable of promoting the assembly of bipolar spindles that do not include centrosomes or chro

276 minority of CDH1-m11 cells arrest with short bipolar spindles that fail to progress to anaphase; this

277 clustering of extra centrosomes into pseudo-bipolar spindles, thereby ensuring viable cell division.

278 tubules emanating from opposing sides of the bipolar spindle through large protein complexes called k

279 form the poles that direct the assembly of a bipolar spindle, thus ensuring the accurate segregation

280 simple two-motor model could create stable, bipolar spindles under a wide range of physical paramete

281 le (MT) plus ends from opposite poles of the bipolar spindle via kinetochores.

282 te chromosomes and simultaneously assemble a bipolar spindle, we developed a computational model of f

283 cient cells are unique in that they assemble bipolar spindles when the pole focusing activities of Nu

284 m egg extracts yields weakened and elongated bipolar spindles which fail to align chromosomes.

285 ganize their microtubule cytoskeleton into a bipolar spindle, which moves one set of sister chromatid

286 task of the SPB is to ensure assembly of the bipolar spindle, which requires a proper balancing of fo

287 cle-treated control cells exhibited a normal bipolar spindle with chromosomes aligned along the metap

288 ipolarity but resulted in larger-than-normal bipolar spindles with a misalignment of chromosomes.

289 king S360 phosphorylation (S360D) results in bipolar spindles with a normal number of microtubules bu

290 Moderately affected embryos contained bipolar spindles with dense and long astral microtubule

291 such as multipolar spindles and asymmetric, bipolar spindles with lagging chromosomes.

292 , whereas metaphase cells exhibited aberrant bipolar spindles with Mad2 localization at kinetochores

293 Multipolar spindles and bipolar spindles with misaligned chromatin are also indu

294 Analysis of experimentally generated bipolar spindles with only one centrosome, as well as of

295 However, short bipolar spindles with organized poles formed after pertu

296 e slide-and-cluster mechanism robustly forms bipolar spindles with sharp poles and a stable steady-st

297 motors pulling on astral microtubules align bipolar spindles with the interphase long cell axis, wit

298 ear how multiple chromosomes form one shared bipolar spindle without centrosomes.

299 One is how to organize a bipolar spindle without microtubule organizing centers a

300 Cells that efficiently maintain bipolar spindles without Eg5 have more stable K-MTs than