ライフサイエンスコーパス: blastema

1 or to formation of a regenerative outgrowth (blastema).

2 pidermis as well as endothelial cells of the blastema.

3 nitor cell recruitment into the regenerative blastema.

4 riction when passed through the regeneration blastema.

5 rom the same mother cell in the regeneration blastema.

6 imal blastema than in a contralateral distal blastema.

7 involves the formation of a mammalian digit blastema.

8 ce of a mass of progenitor tissue called the blastema.

9 al for the formation of wound epithelium and blastema.

10 reased proliferation within the regeneration blastema.

11 nd maintenance of progenitor tissue called a blastema.

12 on at the wound site, forming a regeneration blastema.

13 ng, reducing or positioning the regeneration blastema.

14 ocked fgf-8 but not fgf-10 expression in the blastema.

15 nteractions between the ureteric bud and the blastema.

16 tation at the wound margin, and later in the blastema.

17 stema is equivalent to an amputation-induced blastema.

18 g progenitor cells known as the regeneration blastema.

19 proliferation of the progenitor cells of the blastema.

20 ation and differentiation of the metanephric blastema.

21 l regeneration epithelium, and does not form blastema.

22 s in uninduced metanephrogenic mesenchyme or blastema.

23 y a key role in the redifferentiation of the blastema.

24 m), failed to form a functional regeneration blastema.

25 rmation of digit cartilage from the skeletal blastema.

26 population of type II fibers in regenerating blastema.

27 wth of the ureteric bud into the metanephric blastema.

28 al formation of a mesenchymal growth zone or blastema.

29 -restricted domains and is not a homogeneous blastema.

30 a mass of lineage-restricted cells called a blastema.

31 mulation of proliferating cells known as the blastema.

32 tissue organization within the regeneration blastema.

33 hages failing to infiltrate the regeneration blastema.

34 he post-injury proliferative mass called the blastema.

35 (PD) positional information and assemble the blastema.

36 ail organ and is mediated by a proliferative blastema.

37 ters were equivalent in contralateral distal blastemas.

38 same for both ectopic and amputation-induced blastemas.

39 with fused heads forming in single anterior blastemas.

40 CT formation in lizard (Anolis carolinensis) blastemas.

41 for Wnt-mediated specification of posterior blastemas.

42 amanders capable of limb regeneration form a blastema (a mass of lineage-restricted progenitor cells)

43 ges of regeneration these amphibians form a "blastema", a group of mesenchymal progenitor cells that

44 fically induced in the proximal regeneration blastema, a group of cells that normally proliferate int

45 eration proceeds by the local formation of a blastema, a growth zone of mesenchymal stem cells on the

46 Central to this regenerative process is the blastema, a heterogeneous population of lineage-restrict

47 The blastema, a mass of cells that uniquely forms following

48 rate injured fins through the formation of a blastema, a mass of proliferative cells that can grow an

49 eneration proceeds by local formation of the blastema, a mesenchymal growth zone which normally only

50 osure in the absence of macrophages promotes blastema accumulation, it does not rescue cell different

51 s typically restricted from the regeneration blastema after an amputation injury.

52 umed that limb regeneration derived from the blastema, an undifferentiated pluripotent cell populatio

53 ation, with C3 being expressed mainly in the blastema and C5 exclusively in the wound epithelium.

54 d with reduced proliferation in the proximal blastema and expansion of the nonproliferative distal bl

55 pressed in the dedifferentiated regeneration blastema and in the redifferentiated limb tissues in the

56 ased the oxygen consumption rate of the aged blastema and increased WNT signaling, leading to enhance

57 sufficient for establishing the metanephric blastema and inducing the ureteric bud formation but not

58 A dedicated epidermis overlays the blastema and is required for its proliferation and patte

59 oration is sufficient to rescue a denervated blastema and regenerate the distal structures.

60 is way and found to be expressed in the limb blastema and regulated by retinoic acid.

61 beta-catenin mutations are observed in both blastema and stromal components of WT, current models as

62 n of specialized bone-secreting cells in the blastema and suggest shh expression may be controlled by

63 ing myofibers contribute to the regeneration blastema and that the local extracellular environment pr

64 ings together the positional identity of the blastema and the classical nerve dependence of limb rege

65 , the cell lineage(s) that contribute to the blastema and their ultimate contribution(s) to the regen

66 ng multipotent progenitors that populate the blastema and then give rise to multiple cell types of th

67 Cells at the site of amputation form a blastema and then proliferate and differentiate to regro

68 cell lineages, to generate fate maps of the blastema and to identify the progenitors of regenerated

69 ve determined that formation of both ectopic blastemas and amputation-induced blastemas is regulated

70 d stimuli and generate progeny that can form blastemas and differentiate; yet, they are unable to sel

71 tructs were electroporated into axolotl limb blastemas and the wild type promoter was more active in

72 ng of over 38,000 cells from mouse digit tip blastemas and unamputated control digit tips and generat

73 including within newly generated tissue (the blastema), and along pre-existing intestinal branches un

74 rowth and differentiation of the metanephric blastema, and constitutively activated STATs facilitate

75 -regulation is required for formation of the blastema, and its up-regulation is necessary for the red

76 racteristics, formation of an msx-expressing blastema, and neurogenesis.

77 The early regenerated lizard tail forms a blastema, and the regenerated skeleton consists of a car

78 ogenitor cell population to the regeneration blastema, and these progenitor cells subsequently restor

79 induce formation of both ectopic and normal blastemas, and the diversity of positional information p

80 ify beta1-integrin as a crucial regulator of blastema architecture.

81 ons for many different cell types within the blastema are present at all stages of limb regeneration,

82 on, and proliferation indicates that ectopic blastemas are equivalent to blastemas that form in respo

83 sly1 is upregulated in the newly formed blastema as well as during regenerative outgrowth.

84 tion proceeds with or without formation of a blastema, as observed for the limb and skin, respectivel

85 y little is known about how the regeneration blastema assembles differentiating cells into well-struc

86 analysis revealed a metabolic shift in aged blastema associated with an increased bioenergetic requi

87 ion occurs following decapitation, forming a blastema at the oral pole within 24 hr.

88 rom wild-type and PDGFR-beta -/- metanephric blastemas at 11.5 days post-conception.

89 involved low-level expression of markers of blastema-based regeneration, focused in distal structure

90 e nervous system in pattern formation during blastema-based regeneration.

91 quantify how incorporation into an appendage blastema broadens the progeny contributions of a cellula

92 aled that they are expressed in regenerating blastemas but lost upon denervation.

93 rative response to amputation and form small blastemas but then undergo tissue regression and lysis.

94 2) mutant promoter in contralateral proximal blastemas, but the promoters were equivalent in contrala

95 The blastema can regenerate autonomously as a self-organizin

96 al nerve-derived factor must be found in the blastema, capable of rescuing regeneration in denervated

97 phila Dll, has been isolated from an axolotl blastema cDNA library, and its expression in developing

98 nstrated by hybridization experiments that a blastema cell line of myogenic origin expresses C3.

99 We show that blastema cell migration is stimulated by the cytokine, S

100 onstrate that BMP-2 alone does not influence blastema cell migration, suggesting a requirement of ano

101 mals is not followed by regeneration because blastema cells (BCs) and expression of regenerative gene

102 at tissue stem cells rather than pluripotent blastema cells are an evolutionarily conserved cellular

103 Other studies propose that blastema cells are non-uniform populations that remain r

104 Blastema cells are not derived from stem cells but behav

105 ersity of positional information provided by blastema cells derived from opposite sides of the limb i

106 ish tailfins is encoded within proliferating blastema cells during a critical period of regeneration.

107 roteinase-1 (Mmp1) expressed specifically in blastema cells during disc regeneration.

108 molecular mechanisms, and that both types of blastema cells exhibit the same functions in controlling

109 Blastema cells express both SDF-1alpha receptors, CXCR4

110 beta-Gal is expressed at high levels in blastema cells for about a week and in differentiated ce

111 rom a nerve and a wound epithelium to induce blastema cells from fibroblasts within the wound environ

112 Ihh signaling, whereas distal CTs form from blastema cells in response to Shh signals from regenerat

113 down Piwi1, Vasa, Pl10 or Ncol1 expressed by blastema cells inhibited regeneration but not blastema f

114 In addition, ectopic blastema cells interact coordinately with amputation-ind

115 Most blastema cells maintain their disc-specific identity dur

116 In this study, we focus on how blastema cells respond to BMP signaling during neonatal

117 First, we show that blastema cells retain regenerative properties after expa

118 y signaling from limb amputation to generate blastema cells that can be grafted to the wound, as well

119 sification but by the direct ossification of blastema cells that form the rudiment of the digit tip.

120 nteract coordinately with amputation-induced blastema cells to form a regenerated limb.

121 Conversely, FITC-labeled blastema cells were restricted to distal CT regions.

122 expression is increased during formation of blastema cells, and dysfunction leads to mitochondrial d

123 regeneration is mediated by the creation of blastema cells.

124 on requires the formation and maintenance of blastema cells.

125 R4 signaling by endothelial cells to recruit blastema cells.

126 lasts to produce specified rather than naive blastema cells.

127 icate that NHFs and MWFs separately activate blastema-characteristic genes as well as those genes-rel

128 ericytic phagocyte-conditioned media rescues blastema chondrogenesis and cartilage formation in amput

129 eneration, where stem/progenitor cells (the "blastema") completely regenerate the digit tip after dis

130 These results indicate that the proximal blastema comprises an essential subpopulation of the fin

131 In these triple mutants, the metanephric blastema condenses, and expression of early patterning g

132 mal, as well as specifically in regeneration blastemas, consistent with a role in long-range signalin

133 Here, we show that the blastema consists of two components with markedly distin

134 Blastemas containing vertebrae with intact spinal cords

135 Myotubes in the periphery of the blastema continue to differentiate as muscle; those in t

136 comitantly with nerve deviation, the ectopic blastema continues to grow and forms an ectopic limb.

137 ted that resident cell sources producing the blastema contribute lineage-restricted progeny to regene

138 Surprisingly, no blastema developed at the aboral pole after stolon remov

139 These ectopic anterior blastemas differentiate new brains that establish perman

140 erns in mouse digit regeneration and axolotl blastema differentiation reveals common gene groups for

141 Here, we characterize the devoid of blastema (dob) mutant that fails fin regeneration during

142 nd re-epithelialization is inhibited and the blastema does not form.

143 und healing, but was highly expressed in the blastema during epimorphic fin regeneration after amputa

144 ctivity of stem cells in the mesenchyme (the blastema) during this process.

145 cells contribute to this dermis, but not the blastema, during digit tip regeneration.

146 These blastemas each have an organized structure of lineage re

147 the block to differentiation was at the late-blastema/early-chondroblast stage.

148 g mature tissues of the stump from which the blastema emerges.

149 esembles the embryonic kidney, consisting of blastema, epithelial and stromal components, suggesting

150 induced in basal keratinocytes of the apical blastema epithelium in a pattern that is comparable to i

151 ed to form a cell population in regeneration blastemas expressing Six1/2-2, POU2/3, Eya, Sall and Osr

152 s, our analysis reveals 67 genes enriched in blastema fibroblasts including a novel regeneration-spec

153 ent regions restores regenerative abilities: blastemas form and new heads regenerate in tissues that

154 Certain commonalities, such as blastema formation and basement membrane breakdown at th

155 mputated limbs to beryllium nitrate disrupts blastema formation and causes severe patterning defects

156 ty, and bone development are enriched during blastema formation and development.

157 hogenesis are transiently upregulated during blastema formation and differentiation, including distal

158 cells underlying the wound epidermis during blastema formation and in distal blastemal tissue during

159 ignaling reduced cell proliferation, blocked blastema formation and induced aberrant collagen deposit

160 aling from exogenous glucocorticoids impairs blastema formation and limits regenerative capacity thro

161 eat-shock protein 60 (hsp60) is required for blastema formation and maintenance.

162 transcription factors play key roles during blastema formation and patterning.

163 Our findings indicate that zebrafish fin blastema formation and regenerative outgrowth require Fg

164 ve compounds from NHFs and MWFs could induce blastema formation and remodeling, respectively, and pre

165 n initial wound healing response followed by blastema formation and the regeneration of the digit tip

166 eneration, temporally concomitant with early blastema formation and the secretion of a flexible sac c

167 regeneration model reveals that NHFs enhance blastema formation and vasculogenesis, while MWFs inhibi

168 s localized to the wound epithelium prior to blastema formation and was later strongly expressed in p

169 ately before limb amputation or during early blastema formation blocked limb regeneration but did not

170 ht to establish in vitro conditions to mimic blastema formation by generating different three-dimensi

171 at fibroblast growth factors (Fgfs) initiate blastema formation from fin mesenchyme.

172 Although blastema formation has been well described, the transcri

173 genetic analyses reveal that injury induces blastema formation in Acomys cahirinus.

174 , a Dkk1b-enriched wound epidermis forms and blastema formation is disrupted, compromising regenerati

175 em cells showed that the cellular source for blastema formation is migration of stem cells from a rem

176 The molecular basis of blastema formation is not understood.

177 These data support the idea that blastema formation results from dedifferentiation of int

178 Blastema formation, a hallmark of limb regeneration, req

179 ages of wound healing, dedifferentiation and blastema formation, and have discovered that the express

180 impairs early regenerative events, including blastema formation, but does not affect morphogenesis of

181 Depletion of phagocytes inhibits blastema formation, but treatment with pericytic phagocy

182 fficient signals and conditions that control blastema formation, growth, and pattern formation during

183 y expressed among proliferating cells during blastema formation, its expression becomes restricted to

184 During early blastema formation, proliferating cells are evenly distr

185 sly expressed low levels of Wg also leads to blastema formation, regeneration and transdetermination.

186 ating limb and may play an essential role in blastema formation, thus providing insight into the long

187 amputation, various cell types contribute to blastema formation, where each cell type retains fate re

188 the induction of mesenchymal stem cells and blastema formation, whereas mps1 is required at a later

189 immediately following fin amputation blocks blastema formation, without obvious effects on wound hea

190 ration, but is not absolutely required after blastema formation.

191 upregulated after fin amputation and before blastema formation.

192 ly if activation occurs during wound healing/blastema formation.

193 opriate time and place to play a role during blastema formation.

194 initiating fin regeneration, and controlling blastema formation.

195 regeneration failed in nbl due to defective blastema formation.

196 lastema cells inhibited regeneration but not blastema formation.

197 In the early stages of regeneration, a blastema forms.

198 m a mound of undifferentiated cells called a blastema, found just below the site of amputation.

199 Building a blastema from the stump is a key step of salamander limb

200 n of mature cells to a condensed mesenchymal blastema, from which replacement tissues develop.

201 r attracting nerves that promote mesenchymal blastema growth, leading to the regeneration of the digi

202 By genetic lineage analyses, the digit tip blastema has been defined as a population of heterogeneo

203 es, however, do not comprehensively evaluate blastema heterogeneity or address lineage restriction of

204 In addition to the modifications of blastema homeostasis, mutations in the three genes alter

205 associated with the transient formation of a blastema, however the formation of a regeneration blaste

206 re heat-shocked following the formation of a blastema, however, they retained the ability to regenera

207 ema, however the formation of a regeneration blastema in mammals is poorly understood.

208 tous expression of wg induces a regeneration blastema in the dorsal aspect of the leg disc.

209 These data imply that the blastema in WTs has progressed to the committed stage in

210 ically induces ectopic anterior regeneration blastemas in posterior and lateral wounds.

211 Ectopic expression of shh or bmp2 in the blastema-induced excess bone deposition and altered patt

212 6B1 inhibition molecularly reprograms distal blastemas into a more proximal identity, phenocopying th

213 dele limb or teleost fin, the formation of a blastema is a crucial step in facilitating subsequent re

214 igit tip, supporting the hypothesis that the blastema is a heterogeneous pool of progenitor cells.

215 tion led to the notion that the regeneration blastema is a homogeneous population of proliferating ce

216 The blastema is a mass of progenitor cells responsible for r

217 The blastema is a mass of progenitor cells that constitute a

218 The blastema is a mass of progenitor cells that enables rege

219 The digit blastema is comprised of proliferating cells that expres

220 development, the formation of a regeneration blastema is controlled by early events that are unique t

221 nerve dependency indicating that an ectopic blastema is equivalent to an amputation-induced blastema

222 First a blastema is formed, comprised of progenitor cells that e

223 healing occurs, ectopic wg is induced and a blastema is formed.

224 skin wound on the side of a limb, an ectopic blastema is induced.

225 regeneration, and this activity of RA on the blastema is observed in two contexts.

226 ion of cells or lineages in the regeneration blastema is often postulated.

227 oth ectopic blastemas and amputation-induced blastemas is regulated by the same molecular mechanisms,

228 roliferation and increased cell death in the blastema leading to a significant retardation of regener

229 ion between the ureteral bud and metanephric blastema leads to renal hypodysplasia, vesicoureteral re

230 Hence, this study aimed to create blastema-like cells (BlCs) by overexpressing Msx1 and Ms

231 ressed node and primitive streak by a caudal blastema-like mass of mesenchyme known as the tail bud.

232 It is accompanied by the formation of a blastema-like structure and the re-growth of multiple ti

233 he transient expression of established digit blastema marker genes.

234 nchymal boundary and later overlaps with the blastema marker msxb.

235 Together, our data unite blastema-mediated regeneration in spiny mice with regene

236 acterize the signaling pathways that control blastema morphogenesis and limb regeneration.

237 We further demonstrate that the regeneration blastema moves as a sweep of proliferation, in which cel

238 a chemical mutagenesis screen to identify no blastema (nbl), a zebrafish mutant with an early fin reg

239 egation and formation of the prechondrogenic blastema occurred normally, and that the block to differ

240 ation is characterized by the formation of a blastema of proliferating cells that appear undifferenti

241 sfection to express ectopically genes in the blastema of regenerating fins.

242 comparative RNA-seq analysis of regenerating blastemas of axolotl and Polypterus reveals the activati

243 The possibility of inducing a blastema on a mammalian limb cannot be discounted, altho

244 Blastemas only generate structures distal to their origi

245 ation can be localized (e.g., a regeneration blastema) or diffuse (compensatory growth).

246 Taken together, our data show that normal blastema organogenesis cannot occur without timely infil

247 ess, we performed deep RNA sequencing of the blastema over a time course in the axolotl, a species wh

248 However, directing Myc expression to the blastema overcomes repression of multiple genes, includi

249 How blastema pattern is generated and integrated with pre-ex

250 Subsequently, blastema pattern is organized around the anterior pole.

251 erior-posterior (AP) and medial-lateral (ML) blastema patterning [2-4].

252 range signaling relevant to specification of blastema positional identity.

253 Since nerve fibers grow into the blastema preceding the appearance of any differentiated

254 the nephric duct adjacent to the metanephric blastema prior to the outgrowth of the ureteric bud.

255 ge response mechanism that is evident during blastema proliferation (early- to late-bud) and studied

256 erning, in part through modulating levels of blastema proliferation.

257 ated with different cell densities along the blastema proximal-distal axis, which correlate with alte

258 Posterior-facing blastemas regenerate a head instead of a tail in Smed-be

259 After bisection, one blastema regenerates a head, while the other forms a tai

260 xolotl, they show vividly which cells of the blastema remember their fate and position of origin.

261 The blastema replaces structures appropriate to its proximod

262 ughout the undifferentiated limb bud and the blastema, respectively, and (4) it is expressed only in

263 plications, the fibroblast stem cells of the blastema responded showing that they are capable of tran

264 ion between the ureteral bud and metanephric blastema resulting in CAKUT.

265 First, exposure to RA proximalizes a distal blastema resulting in duplication of structures proximal

266 generation initiates with the formation of a blastema similar to that observed in regenerating amphib

267 ng tetrapod limbs and regenerating amphibian blastema, Sonic hedgehog is expressed in the posterior m

268 ver, Lgr6-expressing cells contribute to the blastema, suggesting a potential direct role for Lgr6-ex

269 type promoter was more active in a proximal blastema than in a contralateral distal blastema.

270 e in uninduced metanephrogenic mesenchyme or blastema than in their differentiated structures.

271 ial creation by an embryonic primordium, the blastema that emerges at the injury site fashions a clos

272 by a proliferation boost in the mesenchymal blastema that is controlled precisely in time and space.

273 h the recruitment of progenitor cells into a blastema that rebuilds the lost tissue.

274 induces a local source of Ec within the wing blastema that sustains a transcriptional signature neces

275 tes that ectopic blastemas are equivalent to blastemas that form in response to limb amputation.

276 Blastemas that have been injected with vaccinia at diffe

277 h has no effect on the amount of new tissue (blastema) that is regenerated yet produces regenerates w

278 luster at the anterior tip of planarian head blastemas (the anterior pole) is required for anterior-p

279 Digit tip regeneration is mediated by the blastema, the same structure invoked during limb regener

280 Within the newly formed blastema, the spatial coordinates of connective tissue p

281 In 2- and 3-week regenerating blastema, three fiber types were clearly discerned.

282 ent from wild type in contralateral proximal blastemas, thus contrasting with the site 1 results in A

283 onsistent with RNA sequencing data) or early blastema tissue.

284 Second, after transplantation of a distal blastema to a proximal stump, the transplanted cells nor

285 ata and the respecification of the posterior blastema to an anteriorized fate by GJC loss-of-function

286 arison of RNA-seq data from early Polypterus blastema to single-cell RNA-seq data from axolotl limb b

287 (RNA-seq) analysis of Polypterus and axolotl blastemas to provide support for a common origin of pair

288 A popular notion is that cells of the blastema transdifferentiate to different fates during li

289 The blastema ultimately gives rise to all of the tissues in

290 regenerating fin consists of many repeating blastema "units" dedicated to each fin ray.

291 situ, we found undifferentiated regenerative blastemas were less stiff than differentiated stump musc

292 n many organisms involves the formation of a blastema, which differentiates and organizes into the ap

293 te from a precursor structure arising in the blastema, which undergoes extensive branching morphogene

294 Neoblast progeny generate new cells of blastemas, which are the regenerative outgrowths at woun

295 lized hubs presage the site of the manubrium blastema, whose growth is Wnt/beta-catenin dependent and

296 In some, but not all fragments, the blastema will replace missing structures and a few cells

297 ta1-integrin(RNAi) animals formed small head blastemas with severe tissue disorganization, including

298 nstrate that Eya 1 specifies the metanephric blastema within the intermediate mesoderm at the caudal

299 ied to specify the metanephric mesenchyme or blastema within the intermediate mesoderm, the earliest

300 formation of the proliferative regeneration blastema, yet modified the lengths and widths of regener