ライフサイエンスコーパス: blastocyst

1 ays were inhibited (from 3.1 to 1.6 pmol/100 blastocysts).

2 n accurately organizing the developing mouse blastocyst.

3 ining cellular plasticity and organizing the blastocyst.

4 ithin the inner cell mass (ICM) of the mouse blastocyst.

5 lates the formation of the mouse pluripotent blastocyst.

6 rimitive endoderm upon reintroduction to the blastocyst.

7 on in the inner cell mass (ICM) of the mouse blastocyst.

8 ion of cell lineages that comprise the mouse blastocyst.

9 regulates lineage specification of the mouse blastocyst.

10 ed in the inner cell mass (ICM) of the mouse blastocyst.

11 cells and 20% in the inner cell mass of the blastocyst.

12 d to facilitate receptivity to an implanting blastocyst.

13 idual nuclei from the 4-cell stage up to the blastocyst.

14 ntil the 8-cell stage can generate an entire blastocyst.

15 the skewed lineage specification of Klf5 KO blastocysts.

16 les to determine their receptive function to blastocysts.

17 gnificantly decreased the apoptosis index of blastocysts.

18 n and methylation patterns closer to in vivo blastocysts.

19 ted arrested embryos and excluded cells from blastocysts.

20 ssed in the trophectoderm of human and mouse blastocysts.

21 reduced to <2% in the majority (79%) of PNT blastocysts.

22 generated by injecting these mESCs into host blastocysts.

23 iated trophoblast stem cells derived from KO blastocysts.

24 ric contribution of these cells to non-human blastocysts.

25 onic stem cells (FX-hESCs), derived from FXS blastocysts.

26 nges in up to 33% (ZFNs) and 46% (TALENs) of blastocysts.

27 PSCs and the inner cell mass (ICM) of human blastocysts.

28 yonic stem cell lines generated from Emb-LPD blastocysts.

29 nserved and better resemble their respective blastocysts.

30 ared transcriptional similarity with natural blastocysts.

31 cysts, and of epiblast and hypoblast in late blastocysts.

32 damage are prevented from incorporation into blastocysts.

33 emerge as a naive founder population in the blastocyst, acquire capacity for germline and soma forma

34 ty with a robust outcome: the formation of a blastocyst, an organised multi-layered structure ready f

35 cy factors maintain uncommitted cells of the blastocyst and embryonic stem cells in the pluripotent s

36 one of the earliest hormones produced by the blastocyst and has potent immune modulatory effects, esp

37 We apply SCALE to mouse blastocyst and human fibroblast cells and find that cis

38 rresponding to the epiblast of the diapaused blastocyst and indicate that mTOR regulates developmenta

39 e derive from the polar trophectoderm of the blastocyst and persist through early gestation (to E8.5)

40 and internal primitive endoderm (PE) in the blastocyst and subsequently give rise to chorio-allantoi

41 govern the interaction between the competent blastocyst and the receptive uterus.

42 17(+) endoderm progenitors to integrate into blastocysts and contribute to chimeric embryos.

43 he reconstructed SCNT embryos developed into blastocysts and ES cells capable of contributing to trad

44 tal failure in implanted beta1 integrin-null blastocysts and found that primitive endoderm cells are

45 he fragmentation rate and apoptotic index of blastocysts and increased their total cell number.

46 ent of both embryonic stem cells (ESCs) from blastocysts and iPSCs from somatic cells [10, 12].

47 typic and molecular outcomes in Rab11a(null) blastocysts and mouse embryonic fibroblasts.

48 ain a gene expression signature of diapaused blastocysts and remain pluripotent.

49 ergence of epiblast cells in preimplantation blastocysts, and ceasing during human embryonic stem cel

50 f inner cell mass and trophectoderm in early blastocysts, and of epiblast and hypoblast in late blast

51 Indeed, c-Myc is depleted in diapaused blastocysts, and the differential expression signatures

52 rief phase of endometrial receptivity to the blastocyst--and were released into the endometrial fluid

53 as when injected into pre-implantation-stage blastocysts, apparently because the injected cells under

54 echanisms involved in Cdx2 expression in the blastocyst are also utilized in the postimplantation emb

55 The three cell types of the mammalian blastocyst are generated in precise proportions over a s

56 However, male and female blastocysts are similarly hypomethylated, indicating tha

57 d that TE-deprived ICMs derived from 32-cell blastocysts are still able to reconstruct TE during in v

58 that sub-chromosomal abnormalities in human blastocysts arise from mitotic errors in around 70% of c

59 Here we use the mouse blastocyst as a model to unravel a key role of fluid-fil

60 cells (ESCs) derived from Chd7 mutant mouse blastocysts as a tool to investigate roles of CHD7 in ne

61 re to attain uterine receptivity will impede blastocyst attachment and result in a compromised pregna

62 evaginations, defective crypt formation, and blastocyst attachment, leading to severely compromised p

63 1A), a BMP type 1 receptor, is necessary for blastocyst attachment.

64 Upon injection into blastocyst, BCL2-expressing EpiSCs contributed to all bo

65 l) embryos were able to properly form normal blastocysts but died at peri-implantation stages.

66 XY(d-1) females produced substantially more blastocysts but fewer than XO controls.

67 F chromatin remodeling complex in individual blastocysts but not in cultured cells.

68 raft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implan

69 CHD4 can form a morphologically normal early blastocyst, but are unable to successfully complete the

70 lation and second as a major methyl donor in blastocysts, but its origin is unknown.

71 e derived from primed hESCs or directly from blastocysts, but their X chromosome state has remained u

72 evels of H3K9me2 and H3K9me3 in SCNT-derived blastocysts, but this was unable to improve their preimp

73 yos as they developed from morula to hatched blastocysts by a progressive increase in the lactate/oxy

74 nstrates that signalling insights from human blastocysts can be used to define culture conditions tha

75 ealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional simi

76 The total blastocyst cell numbers were counted, and the fragmentat

77 distinct, with 2i being the most similar to blastocyst cells and including a subpopulation resemblin

78 ry, which leads to trophectoderm rupture and blastocyst collapse.

79 Blastocysts collected from Emb-LPD mothers within standa

80 nctional lungs in mice through a conditional blastocyst complementation (CBC) approach that vacates a

81 mouse embryonic stem cells (ESCs) via neural blastocyst complementation (NBC).

82 ues, resulting in whole-organ generation via blastocyst complementation across wide evolutionary dist

83 provide proof-of-principle for interspecific blastocyst complementation as a viable approach for kidn

84 Previously, we demonstrated by interspecific blastocyst complementation between mouse and rats, gener

85 Interspecies blastocyst complementation enables organ-specific enrich

86 Interspecies organ generation via blastocyst complementation has succeeded in rodents, but

87 potential of PSC-derived islets generated by blastocyst complementation in a xenogeneic host.

88 bryogenesis, and human disease, interspecies blastocyst complementation might allow human organ gener

89 Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9

90 ditional cell ablation approach with a novel blastocyst complementation strategy, we generated murine

91 Blastocyst complementation with wild-type porcine blasto

92 ion, 3-dimensional printing and interspecies blastocyst complementation, promise organoids manufactur

93 vels of organ chimerism can be generated via blastocyst complementation.

94 rowing human organs through the technique of blastocyst complementation.

95 em cells (PSCs) in mice through interspecies blastocyst complementation.

96 erived iPS cells to reform hematopoiesis via blastocyst complementation.

97 Mammalian blastocysts comprise three distinct cell lineages essent

98 se development leads to the formation of the blastocyst consisting of the inner cell mass (ICM) and t

99 be propagated in vitro and, when placed into blastocysts, contribute to all tissues of the embryo and

100 inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives

101 Here, blastocysts could be recovered from progesterone-induced

102 N + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medi

103 Total SAM levels in mouse blastocysts decreased significantly only when both pathw

104 been transferred into Mstn(tm1Sjl/+) dams as blastocysts demonstrated that the effects of maternal my

105 labeled wild-type embryonic stem cells into blastocysts derived from lipodystrophic A-ZIP transgenic

106 Blastocyst-derived embryonic stem cells (ESCs) and gonad

107 TSCs derived from naive hPSCs are similar to blastocyst-derived hTSCs and acquire features of post-im

108 d embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic

109 s transcriptional profiles highly similar to blastocyst-derived TSCs, with comparable methylation and

110 profiles, and DNA methylation patterns with blastocyst-derived TSCs.

111 h significant decline in oocyte cleavage and blastocyst development after in-vitro fertilization.

112 growth, induces reversible pausing of mouse blastocyst development and allows their prolonged cultur

113 uggest that Rab11a critically controls mouse blastocyst development and soluble matrix metalloprotein

114 gated the parameters affecting efficiency of blastocyst development and stem-cell derivation.

115 tochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro,

116 5F1) in diploid human zygotes and found that blastocyst development was compromised.

117 ned the expression of orthologous genes, and blastocyst development was established, but maintenance

118 uced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard n

119 N at 50% of standard concentrations restored blastocyst development, hatching, and cell number.

120 twofold increase in lumenal pressure during blastocyst development, which translates into a concomit

121 commit to either fate asynchronously during blastocyst development.

122 elf-renewal, somatic cell reprogramming, and blastocyst development.

123 ockdown of ATXN2L led to a 22.9% increase in blastocyst development.

124 the morula stage and cell sub-populations in blastocysts, differential histone modification expressio

125 ls of the inner cell mass (ICM) of the mouse blastocyst differentiate into the pluripotent epiblast o

126 the absence of a functional TE, Chd4 mutant blastocysts do not implant and are hence not viable.

127 here it blocks the transition from morula to blastocyst during embryonic development.

128 h all somatic H1 subtypes are present in the blastocyst, each stage of preimplantation development is

129 Following Myc inhibition, pre-implantation blastocysts enter biosynthetic dormancy but can progress

130 lly diapaused blastocysts in vivo and paused blastocysts ex vivo display pronounced reductions in mTO

131 Fgf4 heterozygous blastocysts exhibited increased numbers of NANOG-positiv

132 Blastocysts exhibited transcriptome profiles more closel

133 ine-rich splicing factor 3) is essential for blastocyst formation and for proper liver development an

134 Here, we further explore the role of Oct4 in blastocyst formation and specification of epiblast versu

135 Blastocyst formation from human SCNT embryos occurs at a

136 t zygote viscoelastic properties can predict blastocyst formation in humans and mice within hours aft

137 The process of blastocyst formation is one of the best-known examples o

138 pression of KDM4A significantly improves the blastocyst formation rate in human SCNT embryos by facil

139 es of success for zygote cleavage and embryo blastocyst formation.

140 stabilizes earlier than the ICM and prior to blastocyst formation.

141 their embryos are less likely to survive to blastocyst formation.

142 inger proteins (KZNFs) recruit KAP1 prior to blastocyst formation.

143 eneration, and glycolysis is dispensable for blastocyst formation.

144 Mouse embryonic stem cells (ESCs), like the blastocyst from which they are derived, contain precurso

145 me profiles more closely resembling those of blastocysts from developmentally competent oocytes.

146 metabolic disorders that were identified in blastocysts from mitochondrial DNA deficient oocytes.

147 differentiation but blocked hatching of the blastocysts from the zona pellucida.

148 the naive pluripotent epiblast of the mouse blastocyst generates a rosette, undergoes lumenogenesis

149 The inner cell mass of the mouse blastocyst gives rise to the pluripotent epiblast (EPI),

150 RNA sequencing of cells from cultured human blastocysts has enabled us to define the transcriptomic

151 as well as generation of forces required for blastocyst hatching.

152 son to inner cell masses of marmoset primate blastocysts identifies a similar complement of pluripote

153 ce, their secretions play important roles in blastocyst implantation and stromal cell decidualization

154 e completely infertile because of defects in blastocyst implantation and stromal cell decidualization

155 defects in pregnancy are evident as reduced blastocyst implantation and subsequent defects in stroma

156 uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnan

157 triguingly, i.p. injections of LIF initiated blastocyst implantation in the uteri of both gland-conta

158 Blastocyst implantation is a complex process requiring c

159 In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by d

160 is no confirmatory non-invasive biomarker of blastocyst implantation or pregnancy.

161 ed genes that influence uterine receptivity, blastocyst implantation, and stromal cell decidualizatio

162 tances that regulate uterine receptivity for blastocyst implantation.

163 in vivo, lasting 2-3 days around the time of blastocyst implantation.

164 mouse uterine tissue sections preparing for blastocyst implantation.

165 ls into a cup-shaped epithelium as the mouse blastocyst implants is a poorly understood and yet key d

166 beyond cleavage and morula stages to form a blastocyst in vitro.

167 EPS-blastoids resembled blastocysts in morphology and cell-lineage allocation an

168 We show that both naturally diapaused blastocysts in vivo and paused blastocysts ex vivo displ

169 ral-like particles and Gag proteins in human blastocysts, indicating that early human development pro

170 d a functional assay utilizing interspecific blastocyst injection and in vitro culture (interspecies

171 mbryonic and placental tissues, as seen in a blastocyst injection assay.

172 Blastocyst injection showed that STAP cells efficiently

173 mmed to pluripotency or near-pluripotency by blastocyst injection, by somatic cell nuclear transfer a

174 romosome and contribute to embryos following blastocyst injection, generating germline-competent chim

175 ages, and chimerize host placentas following blastocyst injection.

176 matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells w

177 During embryo implantation, the blastocyst interacts with and regulates the endometrium,

178 The implanting mouse blastocyst invades the uterine stroma and undergoes a dr

179 Implantation of the blastocyst is a developmental milestone in mammalian emb

180 from the inner cell mass (ICM) in the mouse blastocyst is determined by position-dependent Hippo sig

181 mass (ICM) and the trophoblast layer of the blastocyst is known to occur, but its functional consequ

182 luripotency factor in the inner cell mass of blastocysts, is also expressed in unipotent primordial g

183 le such cells are readily derived from mouse blastocysts it has not been possible to isolate human eq

184 that interspecies complementation of rodent blastocysts lacking a developmental regulatory gene can

185 While PBNT zygotes developed to blastocysts less frequently (42%) than controls (75%), g

186 polyploid blastomere, compaction, morula and blastocyst-like stages by light, scanning electron or th

187 iation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through linea

188 riptome profiling analyses reveal that these blastocyst-like structures show distinct embryonic-abemb

189 trophoblast stem cells to self-organize into blastocyst-like structures with all three embryonic and

190 ryonic stem cells (ESCs) into wild-type (WT) blastocysts (mdx/WT chimera).

191 Moreover, blastocyst microinjection showed that the iMuSCs contrib

192 ] and one inner cell mass [ICM] analyzed per blastocyst; n = 390), we determine the origin of the ane

193 We demonstrate that transfer of blastocysts naturally conceived without hormone stimulat

194 ICMs isolated from more advanced blastocysts no longer retain totipotency, failing to for

195 to trophoblast stem cells derived from human blastocysts or first-trimester placentas(7).

196 ng human embryonic stem cell derivation from blastocyst outgrowths.

197 tal cell number in the resulting morulae and blastocysts positively correlated with the zinc spark am

198 In addition, blastocysts produced in RN + PL contained more ICM cells

199 during mammalian embryogenesis arise in the blastocyst, producing the inner cell mass and the trophe

200 ides a twofold increase in the efficiency of blastocyst production after in vitro fertilization.

201 ocyte microinjection reduced lysis, improved blastocyst rate, increased the number of targeted bi-all

202 p < 0.05) resulting in significantly reduced blastocyst rates (p < 0.001).

203 Sperm motility, viability, fertilization and blastocyst rates were lower in Prdx6 (-/-) spermatozoa t

204 parthenotes and zygotes that developed into blastocysts released more zinc than those that failed to

205 Paused blastocysts remain pluripotent and competent-able to giv

206 Their development into blastocysts requires a specific stiffness window.

207 re reproducibly down-regulated in Tet1/3 DKO blastocysts, resulting in a characteristic phenotype of

208 at injecting MVs isolated from ES cells into blastocysts results in an increase in their implantation

209 Single blastocyst RNA-seq reveals key transcriptional changes u

210 Klf5 Furthermore, Klf5 KO and overexpressing blastocysts showed skewed lineage specification phenotyp

211 ell embryos and individual embryonic day 3.5 blastocysts showed unexpectedly variable gene expression

212 ome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in v

213 he oocyte, these aneuploidies persist at the blastocyst stage and the reasons for the high incidence

214 is increased in 16-cell embryos, and by the blastocyst stage cells fail to properly adopt a TE gene

215 In conclusion, DKK1 can act on the morula-to-blastocyst stage embryo to modify subsequent trophoblast

216 of embryonic and extra-embryonic lineages in blastocyst stage embryos, the formation of the three ger

217 isation, and pre-implantation development to blastocyst stage in response to physiological demands in

218 Following IVF embryos were cultured to the blastocyst stage in vitro or transferred to diabetic and

219 e development of the mouse embryo beyond the blastocyst stage in vitro.

220 tion of Cdx2 develop normally until the late blastocyst stage leading to the conclusion that Cdx2 is

221 oid cells is progressively depleted from the blastocyst stage onwards.

222 This promotes efficient development to the blastocyst stage with no detectable effect on aneuploidy

223 g day 5 to 7.5 of development (the morula to blastocyst stage) to promote embryonic elongation on day

224 mpetent to become fertilized, advance to the blastocyst stage, and give rise to live young than their

225 nt of mouse embryos from the morula to early blastocyst stage, based on 4D confocal image volumes.

226 inactive promoters after major ZGA until the blastocyst stage, coinciding with the loss of promoter e

227 ks such as H3K27me3 is achieved by the early blastocyst stage.

228 the ability to regenerate TE up to the early blastocyst stage.

229 n living mouse oocytes and embryos up to the blastocyst stage.

230 loped slower and largely failed to reach the blastocyst stage.

231 early human embryonic development beyond the blastocyst stage.

232 d the percentage of embryos that reached the blastocyst stage.

233 o development and reduces progression to the blastocyst stage.

234 but only if the host embryos are at the pre-blastocyst stage.

235 NANOG and reduced expression of CDX2 at the blastocyst stage.

236 ylase inhibitors, promote development to the blastocyst stage.

237 es to be activated from the four-cell to the blastocyst stage.

238 tic null embryos that develop until the late blastocyst stage.

239 ge, and becomes spatially restricted by late blastocyst stage.

240 ts in defects in TE specification before the blastocyst stage.

241 and severe degeneration before reaching the blastocyst stage.

242 It is critical that epiblast cells within blastocyst-stage embryos receive the necessary regulator

243 toderm and primitive endoderm cells of human blastocyst-stage embryos.

244 med on three 5- to 10-cell biopsies from two blastocyst-stage embryos.

245 fit our model to aneuploidy data from 11,157 blastocyst-stage embryos.

246 ploidies may arrest between the cleavage and blastocyst stages of embryogenesis(5,6), a high number o

247 rom 74 human embryos, spanning the morula to blastocyst stages.

248 s at the four-cell, eight-cell, 16-cell, and blastocyst stages.

249 and CHOP at the 8-cell, 16-cell, morula, and blastocyst stages.

250 y of cis-regulatory elements employed in the blastocyst, stem cell populations and the postimplantati

251 r shed EVs, create an environment within the blastocyst that prevents their premature differentiation

252 njection of ESCs into genetically programmed blastocysts that are subsequently transferred into pseud

253 s after 5 d, and the second starts with late blastocysts that upon dissection of the mural trophectod

254 protocol: the first starts with intact early blastocysts that upon zona removal can attach to the sub

255 Supplementation increased development to blastocyst, the final stage of preimplantation developme

256 Like cells of the blastocyst, the resulting naive cells contained two acti

257 cell lineages are present in the developing blastocyst: the trophectoderm (TE), the epiblast (Epi) a

258 mpared with numerous conventional lines from blastocysts, they had unique gene expression and DNA met

259 HHP promotes competence of vitrified bovine blastocysts through modest transcriptional changes.

260 conceptus peri-implantation development from blastocyst to early postimplantation stages by using an

261 gh-resolution imaging of the transition from blastocyst to egg cylinder.

262 ith the timespan of primate development from blastocyst to gastrulation.

263 lls communicate with trophoblasts within the blastocyst to increase their ability to migrate into the

264 1 individual cells from early and late human blastocysts to delineate dynamic gene-expression changes

265 Transfer of such blastocysts to recipient females doubles mean litter siz

266 of singleton livebirth than did fresh single blastocyst transfer (416 [50%] vs 329 [40%]; relative ri

267 to establish whether elective frozen single blastocyst transfer improved singleton livebirth rate co

268 ngleton livebirth rate than did fresh single blastocyst transfer in ovulatory women with good prognos

269 Frozen single blastocyst transfer resulted in a higher singleton liveb

270 Frozen single blastocyst transfer resulted in higher rates of singleto

271 yndrome (four of 825 [0.5%] in frozen single blastocyst transfer vs nine of 825 [1.1%] in fresh singl

272 increased risk of pre-eclampsia after frozen blastocyst transfer warrants further studies.

273 Frozen single blastocyst transfer was associated with a higher risk of

274 preserved and a delayed frozen-thawed single blastocyst transfer was done.

275 For those assigned to frozen blastocyst transfer, all blastocysts were cryopreserved

276 on livebirth rate compared with fresh single blastocyst transfer.

277 ly assigned to either fresh or frozen single blastocyst transfer.

278 ansfer vs nine of 825 [1.1%] in fresh single blastocyst transfer; p=0.16), pregnancy loss (134 of 583

279 bryos to DKK1 during the period of morula to blastocyst transition (between d 5 and 8 of development)

280 nduced deletion of Oct4 during the morula to blastocyst transition disrupts the ability of inner cell

281 of glucose metabolism to drive the morula to blastocyst transition.

282 xpression blocking development at the morula-blastocyst transition.

283 is regulated through Hippo and Notch in the blastocyst trophectoderm, unexpectedly finding that it i

284 establishment of three cell lineages in the blastocyst: trophectoderm, primitive endoderm, and epibl

285 njecting mouse PSCs into Pdx-1-deficient rat blastocysts, we generated rat-sized pancreata composed o

286 n, fertilization and in vitro development to blastocyst were not affected by ZP4 ablation.

287 assigned to frozen blastocyst transfer, all blastocysts were cryopreserved and a delayed frozen-thaw

288 ETV2-null blastocysts were injected with human induced pluripotent

289 Blastocysts were produced in vitro either without (C-IVF

290 mbryos arrested with 2-4 cells and almost no blastocysts were produced; by contrast, XY(d-1) females

291 Blastocysts were transferred into synchronized recipient

292 consistent with the inner cell mass of human blastocysts, where MYC transcriptional activity is highe

293 ing cancer cell lines into early-stage mouse blastocysts, which induces central immune tolerance.

294 Fertilized eggs develop poorly into blastocysts, which results in significantly decreased fe

295 is expressed in all cell populations of the blastocyst, while Fgfr2 expression becomes restricted to

296 lasmy in a stem cell line derived from a PNT blastocyst with 4% mtDNA carryover.

297 e first generated genetic mosaic ovaries and blastocysts with stochastic expression of wild-type or m

298 We treated bovine blastocysts with three different HHP (40, 60 and 80 MPa)

299 controls, and this variability correlated in blastocysts with variably increased 5mC/5hmC in gene bod

300 Downregulation of blastocyst WNT signals drives the transition into rosett