ライフサイエンスコーパス: blastomere

1 mic fashion unique for each protein and each blastomere.

2 ers and low in the daughters of the anterior blastomere.

3 e, rafts were also apically enriched in each blastomere.

4 orm anterior pharyngeal muscles from the ABa blastomere.

5 d hypodermis, tissues normally made by the C blastomere.

6 ntrated in the late-dividing, two-cell-stage blastomere.

7 s a result of G2 cell cycle arrest of the P4 blastomere.

8 e) determines the developmental fate of each blastomere.

9 in misorientation of the spindle of the ABar blastomere.

10 d, ANE regulatory state to the most anterior blastomeres.

11 MEX-3, enabling ZIF-1 expression in somatic blastomeres.

12 or proper nuclear division in large dividing blastomeres.

13 distributed equally to somatic and germline blastomeres.

14 ely repressing transcription in all germline blastomeres.

15 ly in oocytes and segregated to all germline blastomeres.

16 pendent upon the unequal division of vegetal blastomeres.

17 es high PIE-1 levels in oocytes and germline blastomeres.

18 Similar results are seen with isolated blastomeres.

19 lar programs of the embryo and of individual blastomeres.

20 regulated by the unequal division of vegetal blastomeres.

21 throughout the presumptive endoderm and B7.5 blastomeres.

22 etric distribution of Cdx2 mRNA in polarized blastomeres.

23 at the blastula stage from the most marginal blastomeres.

24 e whole and/or partial chromosomes lost from blastomeres.

25 become the retina by injection into Xenopus blastomeres.

26 scription in both somatic and later germline blastomeres.

27 t mature differently in somatic and germline blastomeres.

28 n that blocks mRNA transcription in germline blastomeres.

29 ad4 homo- and heteromers in isolated Xenopus blastomeres.

30 e structures within the cytoplasm of vegetal blastomeres.

31 erated in the apical membrane of early stage blastomeres.

32 human embryonic stem (hES) cells from single blastomeres.

33 tive pathway both in NSM cells and in animal blastomeres.

34 furrow, and remains enriched in animal pole blastomeres.

35 essential to set the identities of the early blastomeres.

36 ture, including the identity of the columnar blastomeres.

37 ng that hES cells can be derived from single blastomeres.

38 ributed in the clear apical cytoplasm of the blastomeres.

39 dantly to assemble germ granules in germline blastomeres.

40 is seen with features of totipotent two-cell blastomeres.

41 activation of HIPPO signaling in the outside blastomeres.

42 y to suppress HIPPO signaling in the outside blastomeres.

43 olarity proteins to contact-free surfaces of blastomeres.

44 ent of 471 individual samples comprising 254 blastomeres, 42 polar bodies, and 175 cellular fragments

45 egans pharynx is produced from the embryonic blastomeres ABa and MS.

46 otes PtdIns(3,4,5)P(3) synthesis at sites of blastomere adhesion at all cleavage stages.

47 To determine whether blastomere allocation to the two earliest lineages is ra

48 as expected, but surprisingly three somatic blastomeres also remain poor in H3K4me3.

49 indicate that in vivo, PDCD2 is critical for blastomere and ESC maintenance by contributing to the re

50 l inhibition of Xbves activity within the A1 blastomere and its derivatives completely randomizes mov

51 In this study, we first quantify blastomere and nuclear sizes in X. laevis embryos, demon

52 vision orientation of the endomesoderm (EMS) blastomere and the endoderm fate of the posterior EMS da

53 these mechanisms between different notochord blastomeres and also between different rounds of cell di

54 s destabilized/degraded in animal hemisphere blastomeres and became localized to the nuclei of the fo

55 of a small number of interphasic and mitotic blastomeres and displaying an invariant division pattern

56 lar localization of selected Par proteins in blastomeres and epithelial cells during the embryogenesi

57 itosis during the rapid cleavage division of blastomeres and in somatic cells.

58 ciprocal subchromosomal losses/gains between blastomeres and large segmental errors primarily detecte

59 Canonical Wnt signaling in vegetal blastomeres and Nodal signaling in presumptive oral ecto

60 Characteristics of arrested prophase blastomeres and oocytes are the alignment of condensed c

61 EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all

62 pecific transcriptional program in the inner blastomeres and prevents segregation of the TE and ICM l

63 rofile clearly differentiated left and right blastomeres and provides strong support for models in wh

64 150 nonredundant protein groups between all blastomeres and replicate measurements, we found signifi

65 gion of the embryo but is inactive in animal blastomeres and show that the inability of LvDsh to func

66 ginine methyltransferase CARM1 in individual blastomeres and show that this directs their progeny to

67 data suggest heterogeneity among early-stage blastomeres and that the UCSFB lines have unique propert

68 Thirteen uniquely identifiable individual blastomeres and two double cell combination deletions we

69 an be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic

70 adherin, the major cell-adhesion molecule of blastomeres, and present evidence that this modification

71 yos, nos-2 translation is repressed in early blastomeres, and this inhibition depends on a second reg

72 e exchange of cytoplasm and membrane between blastomeres; and (b) they are active in caveolar endocyt

73 the MOEP19 domain was re-established at the blastomere apices.

74 e and fragmentation parameters of individual blastomeres are diagnostic of ploidy, amenable to automa

75 In C. elegans, germline blastomeres are initially kept transcriptionally quiesce

76 Blastomeres are removed from morula (eight-cell)-stage e

77 Thus, the B7.5 blastomeres are the only cells to express sustained leve

78 found in two-cell (2C) embryos in which the blastomeres are totipotent.

79 When CD divides, the larger D and smaller C blastomeres arise invariantly on the left and right side

80 Three-dimensional visualization of blastomere arrangement and diagenetic cement in cleavage

81 , H3K4me3 deposition is poor in the germline blastomere, as expected, but surprisingly three somatic

82 The unequal division of the CD blastomere at second cleavage is critical in establishin

83 on of maternal proteins to the left or right blastomere at the first cell division.

84 d annelids), it has long been known that one blastomere at the four-cell stage, the D cell, and its d

85 ces of progeny cells of edited and nonedited blastomeres at embryonic day 14.5 showed that off-target

86 m specification: appropriate polarisation of blastomeres at the 8- and 16-cell stage and then the mai

87 n of different histone modifications between blastomeres at the morula stage and cell sub-populations

88 sed the experimental potential of individual blastomeres based on their level of Cdx2-eGFP expression

89 human embryos to address the hypothesis that blastomere behaviour may reflect ploidy during the first

90 ation further elevated overall blastomere-to-blastomere biases during the two- to 16-cell embryo stag

91 , we revealed that the initial blastomere-to-blastomere biases emerge as early as the first embryonic

92 rs, showed ever-increasing asymmetry between blastomeres (bistable pattern), supposedly controlled by

93 The C. elegans MS blastomere, born at the 7-cell stage of embryogenesis, g

94 is switch occurs asynchronously in different blastomeres but is independent of clonal cell heritage a

95 epresses transcription in the later germline blastomeres but not in the earlier germline blastomeres

96 in was found at the apex of outer, polarized blastomeres but was undetectable in blastomeres of the i

97 minally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (

98 o block serine 2 phosphorylation in germline blastomeres, but unexpectedly is not essential for trans

99 nos-2 RNA is also degraded in somatic blastomeres by a process that is independent of translat

100 canonical Wnt pathway to the dorsal marginal blastomeres by defining the domain where the Wnt8a activ

101 potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathway

102 ZIF-1 continues to be repressed in germline blastomeres by POS-1, a germline blastomere-specific pro

103 ttern of symmetric/asymmetric divisions of a blastomere can be influenced by its origin in relation t

104 also show that the genetic lineage of early blastomeres can be traced by DNA methylation analysis.

105 g CTNNB1 undergo fission and these separated blastomeres can become small trophoblastic vesicles, whi

106 f all species, yet how neighboring embryonic blastomeres can contribute to different germ layers has

107 ndogenous VegT and/or Vg1 in ventral vegetal blastomeres can induce a neural fate, but only allows ex

108 ntly, injection of Eg5 into TPX2-CT-arrested blastomeres causes resumption of cleavage.

109 Addressing the question of blastomere cell fate may be of practical importance, bec

110 n from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo.

111 nic trophectoderm are established when eight blastomeres compact to form polarized morulae in preimpl

112 m human embryos at the blastomere, polyploid blastomere, compaction, morula and blastocyst-like stage

113 e remarkable genomic heterogeneity among the blastomeres comprising a single embryo during human prei

114 opy number of parental genomes in 116 single blastomeres comprising entire preimplantation bovine emb

115 opposing lineage specifiers within an early blastomere constantly compete with each other based on t

116 een blastomere progeny so that the loss of a blastomere could be compensated for during development.

117 hen the mass of yolk-free proteins in single blastomeres decreased from approximately 0.8 mug (16-cel

118 ge is not exactly symmetric, the content per blastomere decreases roughly by half with each cell divi

119 On the other hand, blastomere deletion experiments indicate that the D quad

120 ding proteins, with expression in a specific blastomere dependent upon the precise combination of the

121 Expression in the blastomeres depends upon a conserved Nodal response elem

122 These blastomere-derived cell aggregates are plated into micro

123 The resulting blastomere-derived outgrowths are cultured in the same m

124 e-cell gene expression analysis reveals that blastomeres develop cell autonomously, with some cells a

125 altered subcellular localization of TEAD4 in blastomeres dictates first mammalian cell fate specifica

126 tomeres, we report highly reproducible inter-blastomere differences among 10 2-cell and five 4-cell m

127 Inter-blastomere differences created coexpression gene network

128 se data substantiate the hypothesis of inter-blastomere differences in 2- and 4-cell mouse embryos, a

129 ing of early embryogenesis, perturbations of blastomere dimensions, and reconstitution in vitro.

130 t progeny of mesoderm and ectoderm producing blastomeres display intra-germ layer compensation.

131 It requires that some blastomeres divide asymmetrically to direct cells to the

132 D-modeling framework that iteratively infers blastomere division positions and orientations, and cons

133 tribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with n

134 ntain long-term contacts between nonadjacent blastomeres during expansion of the interstitial space i

135 modification expression was detected between blastomeres earlier in human embryos at the four- to eig

136 arge cells following zygote formation, early blastomeres employ modified cell divisions.

137 In C. elegans the 4-cell stage blastomere EMS is an endomesodermal precursor.

138 Isolated cleavage blastomeres exhibit polarized actin-dependent fluid phas

139 f either factor in dorsal-animal retinogenic blastomeres expands expression of neural/retinal genes a

140 n response to stabilized betacatenin, dorsal blastomeres express the closely related transcriptional

141 A single mouse blastomere from an embryo until the 8-cell stage can gen

142 By transplanting blastomeres from a wild-type (WT) zebrafish into a lakri

143 Unbiased clustering analyses of blastomeres from such embryos revealed that this relatio

144 tation genetic diagnosis requires removal of blastomeres from the early human embryo.

145 cal and growth-rate heterogeneity, and mouse blastomeres from the same embryo have stochastic differe

146 Controversy exists as to whether individual blastomeres from two-cell-stage mouse embryos have ident

147 Single blastomeres from Xenopus laevis embryos at the 50-cell s

148 Inter-blastomere gene expression differences dominated between

149 ocyst complementation with wild-type porcine blastomeres generated viable chimeric embryos whose hema

150 the 50-cell stage (~200 ng yolk free protein/blastomere) generated 20 943 unique peptides and 2597 pr

151 pindle into the apicobasal axis of polarised blastomeres generates inner and outer cells with differe

152 al to this process, the relationship between blastomere geometry (i.e. shape and position) and the Hi

153 d several hydrogel-based approaches to alter blastomere geometry in cultured embryos.

154 d how in the majority of embryos, individual blastomeres give rise to distinct blastocyst regions.

155 ell division failure in early Xenopus embryo blastomeres has been attributed to a role of maskin in r

156 er, the relationship between PGCCs and giant blastomeres has never been studied.

157 s of DNA synthesis and mitosis, and arrested blastomeres have abnormal spindles, clustered centrosome

158 stomeres, we observed that the blastomere-to-blastomere heterogeneity in 8-, 16-, 32-, and 50-cell em

159 ement to the center of a cell, even in large blastomeres hundreds of microns in diameter.

160 otch signal that specifies embryonic ABa/ABp blastomere identities at the four-cell stage.

161 diversification of anterior-posterior (A-P) blastomere identities.

162 gh we have identified isolated examples of a blastomere imparting a statistically significant bias, w

163 3K4me3 are descendants of the first germline blastomere, implying an activity that impedes on H3K4me3

164 ated in oocytes and localize to one or a few blastomeres in a spatially and temporally dynamic fashio

165 Here we investigate how embryonic blastomeres in C. elegans develop into foregut (pharynx)

166 NPP-16 and CDK-1 function to arrest prophase blastomeres in C. elegans embryos.

167 maging with karyotypic reconstruction of all blastomeres in four-cell human embryos to address the hy

168 hat the orientation of division of polarised blastomeres in the 8- and 16-cell stage embryo determine

169 endoderm (ME) cells are the two most vegetal blastomeres in the early developing embryo of the marine

170 Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contri

171 Removal of any other blastomeres, including PNM progenitors, did not interfer

172 ation dynamics specifically in the posterior blastomere, independently of regulators previously impli

173 dle polarization during division of the ABar blastomere, indicating that these cell surface proteins

174 Using targeted blastomere injection, morpholino-based loss of function

175 fined to half of the embryo via 2-cell stage blastomere injections, the latter does not produce the o

176 noise, and were sufficient to cluster sister blastomeres into distinct groups.

177 ar whether pathways governing segregation of blastomeres into the PSE lineage are conserved.

178 ther study it resulted in differentiation of blastomeres into trophoblast giant cells (TGCs), suggest

179 known if this lineage pattern means a given blastomere is committed to its specific fate, indicative

180 t of clonal cell heritage and of whether the blastomere is within the inner cell mass or the trophoec

181 we performed bottom-up proteomics on single blastomeres isolated by microdissection from 2-, 4-, 8-,

182 0 protein groups were quantified across four blastomeres isolated from a 16-cell embryo.

183 prehensive quantitative proteomics on single blastomeres isolated from these early stage embryos can

184 We propose that single cells (blastomeres) isolated from early stage invertebrate, amp

185 Recently, we derived stem cells with blastomere-like features from mouse cleavage-stage embry

186 hen taken together, our results suggest that blastomere lineage does not impart a widespread bias for

187 We propose that as embryonic blastomeres lose their developmental plasticity, hermaph

188 s, human zygotes and perhaps human embryonic blastomeres may be useful supplements to human oocytes f

189 s able to influence positional allocation of blastomeres, mediating preferential localization to the

190 rmined until the time of formation of the 4d blastomere (mesentoblast).

191 he extent of asymmetry was minimized between blastomeres (monostable pattern), whereas other genes, i

192 The 8-cell stage blastomere MS is primarily a mesodermal precursor, givin

193 the late-dividing but not the first-dividing blastomere of two-cell embryos and, by lineage tracing a

194 o detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR

195 o detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR

196 ricted to a cortical cytoplasmic crescent in blastomeres of 2-, 4- and 8-cell embryos.

197 When blastomeres of 4- to 8-cell stages were dissociated, the

198 tenin revealed differential expression among blastomeres of 8- to 10-cell human embryos.

199 ell lines (designated UCSFB1-10) from single blastomeres of four 8-cell embryos and one 12-cell embry

200 In rapidly dividing blastomeres of medaka (Oryzias latipes) and in somatic c

201 geometry and YAP localisation of individual blastomeres of mouse and human embryos.

202 are fertile even when micromeres, the parent blastomeres of small micromeres, are deleted.

203 ing highly heterogeneously expressed between blastomeres of the 4-cell embryo, with Sox21 showing one

204 fusion proteins are overexpressed in single blastomeres of the 4-cell stage embryo, the progeny of t

205 We found that Vasa protein is present in all blastomeres of the early embryo and that its abundance o

206 of a differentiated oocyte into pluripotent blastomeres of the embryo.

207 olarized blastomeres but was undetectable in blastomeres of the inner cell mass.

208 ymmetry is reversible upon disaggregation of blastomeres of the two- and four-cell embryo.

209 ion of TPX2 or its C terminus (TPX2-CT) into blastomeres of two-cell embryos led to potent cleavage a

210 lso causes CSF arrest in egg extracts and in blastomeres of two-cell embryos.

211 tation techniques to investigate whether the blastomeres of two-cell-stage mouse embryos can reprogra

212 express Pax7, but overexpression of Pax7 in blastomeres of whole embryos that populate the myogenic

213 nerates Ca(2+) transients in the superficial blastomeres of zebrafish blastulae when the nuclear accu

214 onstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos using electroporati

215 inclined 'lineage strength' that pushes the blastomere onto a predisposed, yet flexible, lineage tra

216 egans aggregates are observed in large cells/blastomeres (oocytes, embryos) and in smaller, further d

217 normal-appearing larvae if the prospective D blastomere or 3D macromere is removed.

218 expressed at least two isoforms in the same blastomere or oocyte, which unambiguously demonstrated t

219 by reducing the contractility of interphasic blastomeres or disrupting the establishment of asynchron

220 establish EPSCs from single eight-cell-stage blastomeres or whole eight-cell pre-implantation mouse e

221 By growing the single blastomere overnight, the resulting cells could be used

222 In germline blastomeres, P-bodies are maintained as core granules la

223 In somatic blastomeres, P-bodies recruit the decapping activators L

224 blastomeres but not in the earlier germline blastomeres P0 and P1.

225 ucing a transcriptionally repressed germline blastomere (P1-P4).

226 enerate transcriptionally repressed germline blastomeres (P1-P4) and somatic sisters that become tran

227 We found that the germline precursor blastomere, P4 , fails cytokinesis, leaving a stable cyt

228 In addition, single blastomere PCR analysis revealed mosaicism in CCR5 editi

229 angle of division, such as the position of a blastomere, play a major role in the specification of TE

230 indistinguishable from human embryos at the blastomere, polyploid blastomere, compaction, morula and

231 Interestingly, the three somatic blastomeres poor in H3K4me3 are descendants of the first

232 ion and premature division in early germline blastomeres, processes that are independent of PIE-1 fun

233 The MS blastomere produces one-third of the body wall muscles (

234 elopment, or if regulation can occur between blastomere progeny so that the loss of a blastomere coul

235 Increasing Cdx2 levels in individual blastomeres promotes symmetric divisions, thereby alloca

236 Organizer formation occurs in dorsal blastomeres receiving both maternal Wnt and zygotic Noda

237 ME cells and PNM cells in gastrulation using blastomere recombinations and confocal microscopy.

238 ryos, transcriptional repression in germline blastomeres requires PIE-1 protein.

239 tion, while overnight culture of dissociated blastomeres resulted in formation of re-aggregated embry

240 We find that loss of blastomeres results in compensation.

241 These results demonstrate that blastomeres retain reprogramming activities and support

242 lating early embryonic fate specification by blastomere separations, exposure to lithium, and dominan

243 ing from the major ectodermal progenitor (AB blastomere) several cell divisions later, thereby preven

244 ngth-dependent microtubule forces that probe blastomere shape and yolk gradients, biased by cortical

245 All the other somatic blastomeres show robust deposition of H3K4me3.

246 we show that CCFs and nondividing aneuploid blastomeres showing extensive DNA damage are prevented f

247 Time-lapse imaging of labeled blastomeres shows that the animal cap tissue moves into

248 Germline blastomere-specific localization of PIE-1 depends, in pa

249 in germline blastomeres by POS-1, a germline blastomere-specific protein.

250 o embryonic genome activation, and others to blastomere-specific RNA depletion.

251 gle-cell transcriptome reveal enrichment for blastomere-specific signature and a dynamic DNA methylom

252 failed mitosis/cytokinesis is common in the blastomere stage of the preimplantation embryo.

253 no oligonucleotides (MOs) microinjected into blastomeres suppressed hnRNP K expression from neural pl

254 a asymmetric divisions of eight- and 16-cell blastomeres that allocate cells to inner and outer posit

255 Divisions of polarised blastomeres that allocate polar cells to outer and apola

256 These lobes normally fuse with the blastomeres that give rise to the D quadrant at the two-

257 Altered spindle orientations occurred in blastomeres that had direct contact with one of the ME c

258 <0.2% of the total protein content in single blastomeres that were isolated from the 16-cell frog (Xe

259 c arginine residues are maximal in four-cell blastomeres that will contribute to the inner cell mass

260 bryo is composed of superficially equivalent blastomeres that will generate both the embryonic inner

261 We also find that, as in early somatic blastomeres, the degradation of PIE-1 in Z2/Z3 is facili

262 ) is detected in wild-type-arrested prophase blastomeres, the inactive state is not detected in the a

263 that PGCCs represent somatic equivalents of blastomeres, the most primitive cancer stem cells report

264 TE-specific transcriptional program in inner blastomeres, thereby allowing their maturation toward th

265 In C. elegans, Wnt signaling specifies the E blastomere to become the endoderm precursor.

266 s unsettled whether the contribution of each blastomere to these two lineages can be accounted for by

267 levels of H3 arginine methylation predispose blastomeres to contribute to the pluripotent cells of th

268 , to attenuate Nodal responsiveness and bias blastomeres to ectoderm and mesoderm fates.

269 ess the ability of dorsal-animal retinogenic blastomeres to form retina, converting the lineage from

270 in the vegetal region repress the ability of blastomeres to form retina.

271 es, might modulate the response of embryonic blastomeres to growth factors and other signals that gov

272 NC-120/SRF and HND-1/HAND, can convert naive blastomeres to muscle when overproduced ectopically in t

273 tion, and selection against highly aneuploid blastomeres to overcome chromosome instability during pr

274 ion of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells.

275 pulling forces from neighboring interphasic blastomeres to polarize their shape and thus division or

276 ighlighted by the ability of separated early blastomeres to produce a whole organism.

277 We propose that restriction of early blastomeres to the pharyngeal fate depends on both repre

278 To investigate the contribution of early blastomeres to the veliger larva, we used intracellular

279 iptional activation further elevated overall blastomere-to-blastomere biases during the two- to 16-ce

280 tation embryos, we revealed that the initial blastomere-to-blastomere biases emerge as early as the f

281 different blastomeres, we observed that the blastomere-to-blastomere heterogeneity in 8-, 16-, 32-,

282 Here, by analyzing single-blastomere transcriptome data from mouse and human pre-i

283 Experiments in which blastomere transplantation is combined with foxa MASO tr

284 Clonal loss-of-function, inflicted by blastomere transplantation or gene-transfer assays, high

285 Through blastomere transplantation, we find that tal1 plays a ce

286 We found that blastomeres undergo MR by locally relaxing cortical tens

287 iptome analysis in a single mouse oocyte and blastomere using a deep-sequencing approach.

288 asynchronous cell division, with diminishing blastomere volume as cleavage proceeded.

289 however, by creating embryos with over-sized blastomeres we present evidence of a developmental progr

290 our mRNA-Seq assay with only a single mouse blastomere, we detected the expression of 75% (5,270) mo

291 aring the protein expression among different blastomeres, we observed that the blastomere-to-blastome

292 g deep single-cell RNA-seq of matched sister blastomeres, we report highly reproducible inter-blastom

293 When chromosomes from one of the two blastomeres were replaced with the chromosomes of an emb

294 l lineage specification of MS and its sister blastomere, whereas the inductive interaction promotes t

295 ed reproducible bimodal expression in sister blastomeres, which cannot be explained by random fluctua

296 are required for Mesp expression in the B7.5 blastomeres, which constitute the heart field.

297 single pair of endomesoderm cells, the A6.3 blastomeres, which form part of the anterior endoderm, h

298 ntirely from a single progenitor cell, the E blastomere, whose identity is specified by GATA type tra

299 The relative risk for shrinkage/fusion of blastomeres with subsequent lysis was 1.71 times higher

300 mixture of chromosomally normal and abnormal blastomeres with uniparental or biparental origins.